RLG00000026721

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005396
Physical Location & Seq
Reverse (-)
5292346 .. 5292921
576 bp
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UTR
Exon/CDS
Intron
RLM00000026721

Sequence Viewer

Length: 576 bp
ATGGGAGCTCCCACATTTCTCTCCACTCTTCTCCTATTCATCCTCTTCATCAACACGACCACGGCGACTTCTTCTTCGAGCCAAACCTACAAAACCTACGTCAAAACAGCCTGCAACTCTACCACGTACCCAGAAATCTGCTACAAGGCTCTCTCCTCCTACGCCACATCCGTCAAATCAAACCCTCAGAAGCTCTGCAACTATGCCCTCTCCGTAACCCTAAAAGCCGCCAAAAAAGCCTCTTCCACGGTGTCGAAGCTGTCCAAGAAGAAAGGATTGACGAAAGCAGAGGCCGGGGTGGTGGAGGATTGCATAGAGAACATCGAGGACTCCGTCGACGAGCTCAAACAGGCGGTGACTGCCATGAAAAATTTGGGAGATGCGGATGTCCAACTTCATTTGGATGACATAAAGACTTGGGTTAGTGCTTCGCTGACGGATGAGGACACCTGTACGGATGGATTCGATGAGCAGAAGGTAAGTGCGGCGGTGAAGAACACCATTAATAAGAGCATTCTCACTGTTGCTAGATTGGCTAGCAATGCTTTGTCTCTCATCAACAACCTCAGTTACTAG

Protein Analysis

192

Amino Acids

20.55

Weight (kDa)

7.54

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 185 7.3e-38 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 336
AciI CCGC 5 cut(s) 228, 353, 383, 485, 488
AcsI RAATTY 1 cut(s) 370
AfaI GTAC 2 cut(s) 128, 454
AluBI AGCT 4 cut(s) 8, 193, 259, 343
AluI AGCT 4 cut(s) 8, 193, 259, 343
Alw21I GWGCWC 2 cut(s) 10, 345
Alw26I GTCTC 1 cut(s) 555
AoxI GGCC 1 cut(s) 291
ApoI RAATTY 1 cut(s) 370
ArsI GACNNNNNNTTYG 4 cut(s) 58, 84, 90, 116
AseI ATTAAT 1 cut(s) 504
AsuC2I CCSGG 1 cut(s) 295
AsuHPI GGTGA 2 cut(s) 367, 502
AsuNHI GCTAGC 1 cut(s) 536
BanII GRGCYC 2 cut(s) 10, 345
Bbv12I GWGCWC 2 cut(s) 10, 345
BccI CCATC 1 cut(s) 452
BceAI ACGGC 1 cut(s) 78
BcnI CCSGG 1 cut(s) 295
BcoDI GTCTC 1 cut(s) 555
BfaI CTAG 3 cut(s) 528, 537, 574
BisI GCNGC 2 cut(s) 228, 486
BlsI GCNGC 2 cut(s) 229, 487
Bme1390I CCNGG 1 cut(s) 295
BmrFI CCNGG 1 cut(s) 295
BmsI GCATC 1 cut(s) 370
BmtI GCTAGC 1 cut(s) 540
BpuMI CCSGG 1 cut(s) 295
BsaAI YACGTR 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 60, 246, 294
Bse3DI GCAATG 1 cut(s) 547
BseDI CCNNGG 3 cut(s) 60, 246, 294
BseGI GGATG 6 cut(s) 39, 167, 391, 409, 445, 463
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 145
BshFI GGCC 1 cut(s) 293
BsiHKAI GWGCWC 2 cut(s) 10, 345
BsiSI CCGG 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 555
BsmI GAATGC 1 cut(s) 513
BsnI GGCC 1 cut(s) 293
Bsp1286I GDGCHC 2 cut(s) 10, 345
BspACI CCGC 5 cut(s) 228, 353, 383, 485, 488
BspANI GGCC 1 cut(s) 293
BspCNI CTCAG 1 cut(s) 199
BspOI GCTAGC 1 cut(s) 540
BsrDI GCAATG 1 cut(s) 547
BssECI CCNNGG 3 cut(s) 60, 246, 294
Bst4CI ACNGT 2 cut(s) 250, 523
Bst6I CTCTTC 3 cut(s) 33, 50, 247
BstBAI YACGTR 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 112, 538
BstDEI CTNAG 2 cut(s) 186, 566
BstDSI CCRYGG 2 cut(s) 60, 246
BstF5I GGATG 6 cut(s) 39, 167, 391, 409, 445, 463
BstMAI GTCTC 1 cut(s) 555
BstMWI GCNNNNNNNGC 4 cut(s) 236, 359, 533, 542
BstSCI CCNGG 1 cut(s) 293
BsuRI GGCC 1 cut(s) 293
BtgI CCRYGG 2 cut(s) 60, 246
BtsCI GGATG 6 cut(s) 39, 167, 391, 409, 445, 463
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 2 cut(s) 112, 538
Csp6I GTAC 2 cut(s) 127, 453
CviAII CATG 1 cut(s) 364
CviQI GTAC 2 cut(s) 127, 453
DdeI CTNAG 2 cut(s) 186, 566
Eam1104I CTCTTC 3 cut(s) 33, 50, 247
EarI CTCTTC 3 cut(s) 33, 50, 247
Ecl136II GAGCTC 2 cut(s) 8, 343
Eco24I GRGCYC 2 cut(s) 10, 345
Eco53kI GAGCTC 2 cut(s) 8, 343
EcoICRI GAGCTC 2 cut(s) 8, 343
EcoT38I GRGCYC 2 cut(s) 10, 345
FaeI CATG 1 cut(s) 367
FaiI YATR 4 cut(s) 204, 314, 365, 410
FatI CATG 1 cut(s) 363
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 2 cut(s) 228, 486
FokI GGATG 6 cut(s) 26, 154, 398, 416, 452, 470
FriOI GRGCYC 2 cut(s) 10, 345
Fsp4HI GCNGC 2 cut(s) 228, 486
FspBI CTAG 3 cut(s) 528, 537, 574
GluI GCNGC 2 cut(s) 228, 486
HaeIII GGCC 1 cut(s) 293
HapII CCGG 1 cut(s) 294
Hin1II CATG 1 cut(s) 367
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 2 cut(s) 329, 462
HpaII CCGG 1 cut(s) 294
HphI GGTGA 2 cut(s) 367, 502
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 1 cut(s) 189
Hpy8I GTNNAC 1 cut(s) 337
Hpy99I CGWCG 2 cut(s) 338, 341
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 2 cut(s) 250, 523
HpyCH4IV ACGT 2 cut(s) 99, 125
HpyCH4V TGCA 3 cut(s) 114, 198, 312
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 359, 533, 542
HpyF3I CTNAG 2 cut(s) 186, 566
HpySE526I ACGT 2 cut(s) 99, 125
Hsp92II CATG 1 cut(s) 367
LmnI GCTCC 2 cut(s) 5, 13
LpnPI CCDG 5 cut(s) 124, 144, 307, 335, 463
LweI GCATC 1 cut(s) 370
MaeI CTAG 3 cut(s) 528, 537, 574
MaeII ACGT 2 cut(s) 99, 125
MaeIII GTNAC 3 cut(s) 214, 355, 569
MboII GAAGA 7 cut(s) 20, 37, 63, 66, 234, 280, 505
MhlI GDGCHC 2 cut(s) 10, 345
MluCI AATT 1 cut(s) 370
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 1 cut(s) 415
MseI TTAA 1 cut(s) 504
MslI CAYNNNNRTG 1 cut(s) 402
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 295
Mva1269I GAATGC 1 cut(s) 513
MwoI GCNNNNNNNGC 4 cut(s) 236, 359, 533, 542
NciI CCSGG 1 cut(s) 295
NheI GCTAGC 1 cut(s) 536
NlaIII CATG 1 cut(s) 367
NmuCI GTSAC 1 cut(s) 355
PcsI WCGNNNNNNNCGW 3 cut(s) 62, 168, 330
PctI GAATGC 1 cut(s) 513
PfeI GAWTC 1 cut(s) 462
PflFI GACNNNGTC 1 cut(s) 332
PkrI GCNGC 2 cut(s) 229, 487
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
Ppu21I YACGTR 1 cut(s) 126
PshBI ATTAAT 1 cut(s) 504
Psp124BI GAGCTC 2 cut(s) 10, 345
PsyI GACNNNGTC 1 cut(s) 332
RsaI GTAC 2 cut(s) 128, 454
RsaNI GTAC 2 cut(s) 127, 453
RseI CAYNNNNRTG 1 cut(s) 402
SacI GAGCTC 2 cut(s) 10, 345
SalI GTCGAC 1 cut(s) 335
SaqAI TTAA 1 cut(s) 504
SatI GCNGC 2 cut(s) 228, 486
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 1 cut(s) 295
SduI GDGCHC 2 cut(s) 10, 345
SfaNI GCATC 1 cut(s) 370
SgrDI CGTCGACG 1 cut(s) 335
SmiMI CAYNNNNRTG 1 cut(s) 402
Sse9I AATT 1 cut(s) 370
SsiI CCGC 5 cut(s) 228, 353, 383, 485, 488
SspMI CTAG 3 cut(s) 528, 537, 574
SstI GAGCTC 2 cut(s) 10, 345
StyD4I CCNGG 1 cut(s) 293
TaaI ACNGT 2 cut(s) 250, 523
TaiI ACGT 2 cut(s) 102, 128
TaqI TCGA 5 cut(s) 77, 254, 324, 336, 465
TasI AATT 1 cut(s) 370
TauI GCSGC 2 cut(s) 230, 488
TfiI GAWTC 1 cut(s) 462
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 1 cut(s) 355
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 4 cut(s) 28, 37, 380, 386
TspGWI ACGGA 5 cut(s) 160, 202, 322, 452, 470
TspRI CASTG 1 cut(s) 526
Tth111I GACNNNGTC 1 cut(s) 332
VspI ATTAAT 1 cut(s) 504
XapI RAATTY 1 cut(s) 370
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 336
XspI CTAG 3 cut(s) 528, 537, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.