AT5G51520

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005396
Physical Location & Seq
Forward (+)
20925852 .. 20926783
932 bp
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UTR
Exon/CDS
Intron
AT5G51520.1

Sequence Viewer

Length: 615 bp
ATGTCGCAAGTCCTCTACTCTCTAACGATCGTCTTTGTTTTCTTCGCTTCGACAAACATCCAGAAAACATCAGGCAGTGCATCATCCTACTCCCAAAACCACAAAACGTTCGTGAAAACGGCTTGCAATTCGACAACATACCCCGACAAATGCTACAAGTCCTTATCCTCCTACTCATCGAATATCAAATCCGACCCGATCAAACTATGCACCACCGCGCTTAACCTCAACGTGAAATCCGCAAAGGAAGCAACGTCCGTTGTCTCTAAGCTTCTCAAAATGTCTCAGAAATCAACCGCCGGACGAAAGGGTAAAATGTTACCAGAGGCTCTTATACTCAAGGACTGTCTCGAGGAGATGAAGGACACAATTATTGAGCTCAAGCAAGCGATCACGGAGATGAAGAATCTACAAGATGGTGGCTCTATGGCTGAGCATATAACGAACGTTAGGACGTGGGTGAGCTCGGCGTTGACCGACGAAGGGACTTGTACGGACGGGTTCGAGGAGGTGAAAGTGAATAAAGAAACAAAGAAGAAGGTAAATAAGGTTGTTGAGGAACTTGCAACGACGACTAGTAATACTTTGGCGCTTATTACGAATCTACGTTACTAG

Protein Analysis

204

Amino Acids

22.46

Weight (kDa)

9.09

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 198 2.6e-35 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 218
AciI CCGC 3 cut(s) 216, 240, 297
AclI AACGTT 2 cut(s) 107, 447
AfaI GTAC 1 cut(s) 493
AfiI CCNNNNNNNGG 1 cut(s) 483
AhlI ACTAGT 1 cut(s) 575
AjiI CACGTC 1 cut(s) 456
AluBI AGCT 3 cut(s) 271, 379, 465
AluI AGCT 3 cut(s) 271, 379, 465
Alw21I GWGCWC 2 cut(s) 381, 467
Alw26I GTCTC 3 cut(s) 268, 288, 353
Ama87I CYCGRG 1 cut(s) 350
AspLEI GCGC 2 cut(s) 220, 592
AsuHPI GGTGA 2 cut(s) 472, 523
AvaI CYCGRG 1 cut(s) 350
BanII GRGCYC 2 cut(s) 381, 467
Bbv12I GWGCWC 2 cut(s) 381, 467
BccI CCATC 1 cut(s) 410
BceAI ACGGC 1 cut(s) 135
BcoDI GTCTC 3 cut(s) 268, 288, 353
BcuI ACTAGT 1 cut(s) 575
BfaI CTAG 2 cut(s) 576, 613
BfoI RGCGCY 1 cut(s) 593
BlpI GCTNAGC 1 cut(s) 432
BmeT110I CYCGRG 1 cut(s) 350
BmgBI CACGTC 1 cut(s) 456
BmsI GCATC 1 cut(s) 89
Bpu1102I GCTNAGC 1 cut(s) 432
BpuEI CTTGAG 2 cut(s) 323, 365
Bsc4I CCNNNNNNNGG 1 cut(s) 483
BseGI GGATG 2 cut(s) 57, 83
BseLI CCNNNNNNNGG 1 cut(s) 483
BseMII CTCAG 2 cut(s) 299, 423
BseRI GAGGAG 2 cut(s) 368, 521
Bsh1236I CGCG 1 cut(s) 218
Bsh1285I CGRYCG 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 2 cut(s) 381, 467
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 1 cut(s) 300
BslFI GGGAC 1 cut(s) 499
BslI CCNNNNNNNGG 1 cut(s) 483
BsmAI GTCTC 3 cut(s) 268, 288, 353
BsmFI GGGAC 1 cut(s) 499
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 2 cut(s) 381, 467
Bsp143I GATC 3 cut(s) 27, 198, 390
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 3 cut(s) 216, 240, 297
BspCNI CTCAG 2 cut(s) 298, 424
BspFNI CGCG 1 cut(s) 218
BssMI GATC 3 cut(s) 27, 198, 390
Bst4CI ACNGT 1 cut(s) 347
BstC8I GCNNGC 2 cut(s) 124, 387
BstDEI CTNAG 3 cut(s) 267, 285, 432
BstF5I GGATG 2 cut(s) 57, 83
BstFNI CGCG 1 cut(s) 218
BstH2I RGCGCY 1 cut(s) 593
BstHHI GCGC 2 cut(s) 220, 592
BstKTI GATC 3 cut(s) 30, 201, 393
BstMAI GTCTC 3 cut(s) 268, 288, 353
BstMBI GATC 3 cut(s) 27, 198, 390
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstUI CGCG 1 cut(s) 218
BtrI CACGTC 1 cut(s) 456
BtsCI GGATG 2 cut(s) 57, 83
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 82
Cac8I GCNNGC 2 cut(s) 124, 387
CfoI GCGC 2 cut(s) 220, 592
Csp6I GTAC 1 cut(s) 492
CviJI RGCY 7 cut(s) 122, 271, 329, 379, 423, 431, 465
CviKI_1 RGCY 7 cut(s) 122, 271, 329, 379, 423, 431, 465
CviQI GTAC 1 cut(s) 492
DdeI CTNAG 3 cut(s) 267, 285, 432
DpnI GATC 3 cut(s) 29, 200, 392
DpnII GATC 3 cut(s) 27, 198, 390
Ecl136II GAGCTC 2 cut(s) 379, 465
Eco24I GRGCYC 2 cut(s) 381, 467
Eco53kI GAGCTC 2 cut(s) 379, 465
Eco88I CYCGRG 1 cut(s) 350
EcoICRI GAGCTC 2 cut(s) 379, 465
EcoT38I GRGCYC 2 cut(s) 381, 467
FaiI YATR 6 cut(s) 139, 208, 335, 428, 438, 440
FaqI GGGAC 1 cut(s) 499
FokI GGATG 2 cut(s) 44, 70
FriOI GRGCYC 2 cut(s) 381, 467
FspBI CTAG 2 cut(s) 576, 613
GlaI GCGC 2 cut(s) 219, 591
HaeII RGCGCY 1 cut(s) 593
HapII CCGG 1 cut(s) 300
HhaI GCGC 2 cut(s) 220, 592
Hin6I GCGC 2 cut(s) 218, 590
HinP1I GCGC 2 cut(s) 218, 590
HincII GTYRAC 1 cut(s) 474
HindII GTYRAC 1 cut(s) 474
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 2 cut(s) 406, 601
HpaII CCGG 1 cut(s) 300
HphI GGTGA 2 cut(s) 472, 523
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 2 cut(s) 193, 288
Hpy188III TCNNGA 3 cut(s) 61, 112, 350
Hpy8I GTNNAC 1 cut(s) 474
Hpy99I CGWCG 2 cut(s) 482, 574
HpyAV CCTTC 3 cut(s) 355, 476, 532
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4IV ACGT 6 cut(s) 107, 231, 254, 447, 455, 607
HpyCH4V TGCA 4 cut(s) 80, 126, 210, 566
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 3 cut(s) 267, 285, 432
HpySE526I ACGT 6 cut(s) 107, 231, 254, 447, 455, 607
HspAI GCGC 2 cut(s) 218, 590
Kzo9I GATC 3 cut(s) 27, 198, 390
LpnPI CCDG 4 cut(s) 57, 74, 313, 336
LweI GCATC 1 cut(s) 89
MaeI CTAG 2 cut(s) 576, 613
MaeII ACGT 6 cut(s) 107, 231, 254, 447, 455, 607
MaeIII GTNAC 2 cut(s) 318, 608
MalI GATC 3 cut(s) 29, 200, 392
MboI GATC 3 cut(s) 27, 198, 390
MboII GAAGA 3 cut(s) 34, 415, 547
MhlI GDGCHC 2 cut(s) 381, 467
MluCI AATT 2 cut(s) 127, 369
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 8 cut(s) 23, 178, 236, 319, 346, 499, 502, 550
MseI TTAA 1 cut(s) 222
MslI CAYNNNNRTG 1 cut(s) 398
MspI CCGG 1 cut(s) 300
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 248
NdeII GATC 3 cut(s) 27, 198, 390
NmeAIII GCCGAG 1 cut(s) 446
PaeR7I CTCGAG 1 cut(s) 350
PfeI GAWTC 2 cut(s) 406, 601
Ple19I CGATCG 1 cut(s) 30
Psp124BI GAGCTC 2 cut(s) 381, 467
Psp1406I AACGTT 2 cut(s) 107, 447
PvuI CGATCG 1 cut(s) 30
RsaI GTAC 1 cut(s) 493
RsaNI GTAC 1 cut(s) 492
RseI CAYNNNNRTG 1 cut(s) 398
SacI GAGCTC 2 cut(s) 381, 467
SaqAI TTAA 1 cut(s) 222
Sau3AI GATC 3 cut(s) 27, 198, 390
SduI GDGCHC 2 cut(s) 381, 467
SfaNI GCATC 1 cut(s) 89
Sfr274I CTCGAG 1 cut(s) 350
SlaI CTCGAG 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 398
SmlI CTYRAG 3 cut(s) 338, 350, 380
SmoI CTYRAG 3 cut(s) 338, 350, 380
SpeI ACTAGT 1 cut(s) 575
Sse9I AATT 2 cut(s) 127, 369
SsiI CCGC 3 cut(s) 216, 240, 297
SspMI CTAG 2 cut(s) 576, 613
SstI GAGCTC 2 cut(s) 381, 467
TaaI ACNGT 1 cut(s) 347
TaiI ACGT 6 cut(s) 110, 234, 257, 450, 458, 610
TaqI TCGA 5 cut(s) 50, 131, 179, 351, 504
TaqII GACCGA 1 cut(s) 491
TasI AATT 2 cut(s) 127, 369
TfiI GAWTC 2 cut(s) 406, 601
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 82
TspDTI ATGAA 2 cut(s) 374, 416
TspGWI ACGGA 3 cut(s) 247, 410, 509
TspRI CASTG 1 cut(s) 82
XhoI CTCGAG 1 cut(s) 350
XspI CTAG 2 cut(s) 576, 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.