AT4G33250

ribosome binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008378
Physical Location & Seq
Reverse (-)
16038651 .. 16040808
2158 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33250.1

Sequence Viewer

Length: 681 bp
ATGGGAGTAGAGATCCAATCGCCGCAGGAACAGAGCTCTTACACCGTTGAGCAGCTCGTCGCCCTTAATCCTTTCAACCCTGAAATCCTGCCTGATCTCGAAAACTACGTTAACGTTACATCGCAAACGTATAGTCTGGAAGTAAACCTATGCTTGCTTCGGCTCTATCAGTTTGAGCCTGAGCGCATGAACACTCATATCGTGGCTCGAATCTTGGTCAAGGCTCTTATGGCTATGCCAACTCCAGACTTCAGCCTTTGCCTCTTCTTGATTCCCGAAAGAGTGCAAATGGAGGAGCAGTTCAAGTCACTAATTGTTCTATCACACTACCTTGAGACTGGGAGGTTCCAACAGTTCTGGGATGAAGCTGCCAAGAACCGTCACATTCTCGAGGCTGTTCCAGGTTTTGAGCAAGCTATTCAAGCATATGCAAGTCACCTTCTTAGCTTAAGCTACCAAAAGGTTCCAAGATCAGTGCTGGCTGAGGCTGTTAACATGGATGGTGCATCTCTAGACAAATTCATCGAGCAGCAAGTGACTAACAGTGGATGGATTGTTGAGAAAGAGGGTGGAAGTATTGTCTTACCACAAAATGAATTTAACCATCCGGAGCTCAAGAAAAATACAGGTGAGAATGTTCCCTTGGAACACATTGCCCGCATCTTCCCCATCCTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

226

Amino Acids

25.73

Weight (kDa)

4.92

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CSN8_PSD8_EIF3K PF10075 64 - 195 1.2e-19 CSN8/PSMD8/EIF3K family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 607
AciI CCGC 2 cut(s) 23, 658
AclI AACGTT 1 cut(s) 114
AclWI GGATC 1 cut(s) 7
AcsI RAATTY 2 cut(s) 518, 596
AcuI CTGAAG 1 cut(s) 235
AfiI CCNNNNNNNGG 1 cut(s) 674
AflII CTTAAG 1 cut(s) 448
AgsI TTSAA 3 cut(s) 76, 304, 422
AjnI CCWGG 1 cut(s) 400
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AloI GAACNNNNNNTCC 2 cut(s) 284, 316
AluBI AGCT 7 cut(s) 36, 55, 368, 416, 447, 453, 613
AluI AGCT 7 cut(s) 36, 55, 368, 416, 447, 453, 613
Alw21I GWGCWC 2 cut(s) 38, 615
Alw26I GTCTC 1 cut(s) 329
AlwI GGATC 1 cut(s) 7
Ama87I CYCGRG 1 cut(s) 389
Aor13HI TCCGGA 1 cut(s) 607
ApeKI GCWGC 3 cut(s) 52, 368, 529
ApoI RAATTY 2 cut(s) 518, 596
ArsI GACNNNNNNTTYG 2 cut(s) 118, 150
AspLEI GCGC 1 cut(s) 186
AsuHPI GGTGA 2 cut(s) 428, 641
AvaI CYCGRG 1 cut(s) 389
BanII GRGCYC 2 cut(s) 38, 615
Bbv12I GWGCWC 2 cut(s) 38, 615
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 3 cut(s) 64, 355, 541
BccI CCATC 4 cut(s) 494, 543, 612, 677
BciT130I CCWGG 1 cut(s) 402
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 1 cut(s) 512
BfrI CTTAAG 1 cut(s) 448
BisI GCNGC 4 cut(s) 23, 53, 369, 530
BlsI GCNGC 4 cut(s) 24, 54, 370, 531
Bme1390I CCNGG 1 cut(s) 402
BmeT110I CYCGRG 1 cut(s) 389
BmiI GGNNCC 2 cut(s) 347, 465
BmrFI CCNGG 1 cut(s) 402
BmrI ACTGGG 1 cut(s) 348
BmsI GCATC 2 cut(s) 515, 669
BmuI ACTGGG 1 cut(s) 348
BpmI CTGGAG 1 cut(s) 228
Bpu10I CCTNAGC 2 cut(s) 180, 483
BpuEI CTTGAG 2 cut(s) 353, 599
BsaJI CCNNGG 2 cut(s) 642, 673
BsaWI WCCGGW 1 cut(s) 607
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 674
Bse1I ACTGG 1 cut(s) 343
Bse3DI GCAATG 1 cut(s) 651
BseAI TCCGGA 1 cut(s) 607
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 642, 673
BseGI GGATG 5 cut(s) 367, 505, 554, 604, 669
BseLI CCNNNNNNNGG 1 cut(s) 674
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 2 cut(s) 171, 474
BseNI ACTGG 1 cut(s) 343
BseRI GAGGAG 1 cut(s) 308
BseXI GCAGC 3 cut(s) 64, 355, 541
BsiHKAI GWGCWC 2 cut(s) 38, 615
BsiHKCI CYCGRG 1 cut(s) 389
BsiSI CCGG 1 cut(s) 608
BslI CCNNNNNNNGG 1 cut(s) 674
BsmAI GTCTC 1 cut(s) 329
BsoBI CYCGRG 1 cut(s) 389
Bsp1286I GDGCHC 2 cut(s) 38, 615
Bsp13I TCCGGA 1 cut(s) 607
Bsp143I GATC 3 cut(s) 12, 94, 470
BspACI CCGC 2 cut(s) 23, 658
BspCNI CTCAG 2 cut(s) 172, 475
BspEI TCCGGA 1 cut(s) 607
BspLI GGNNCC 2 cut(s) 347, 465
BspPI GGATC 1 cut(s) 7
BspTI CTTAAG 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 651
BsrI ACTGG 1 cut(s) 343
BssECI CCNNGG 2 cut(s) 642, 673
BssMI GATC 3 cut(s) 12, 94, 470
BssT1I CCWWGG 2 cut(s) 642, 673
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 4 cut(s) 46, 354, 380, 545
Bst6I CTCTTC 1 cut(s) 269
BstAFI CTTAAG 1 cut(s) 448
BstC8I GCNNGC 4 cut(s) 155, 414, 480, 658
BstDEI CTNAG 3 cut(s) 180, 443, 483
BstF5I GGATG 5 cut(s) 367, 505, 554, 604, 669
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 3 cut(s) 15, 97, 473
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 3 cut(s) 12, 94, 470
BstMWI GCNNNNNNNGC 2 cut(s) 230, 422
BstNI CCWGG 1 cut(s) 402
BstSCI CCNGG 1 cut(s) 400
BstV1I GCAGC 3 cut(s) 64, 355, 541
BstX2I RGATCY 1 cut(s) 12
BstYI RGATCY 1 cut(s) 12
BtgZI GCGATG 1 cut(s) 105
BtsCI GGATG 5 cut(s) 367, 505, 554, 604, 669
BtsIMutI CAGTG 2 cut(s) 480, 550
Cac8I GCNNGC 4 cut(s) 155, 414, 480, 658
CfoI GCGC 1 cut(s) 186
CviAII CATG 2 cut(s) 187, 496
DdeI CTNAG 3 cut(s) 180, 443, 483
DpnI GATC 3 cut(s) 14, 96, 472
DpnII GATC 3 cut(s) 12, 94, 470
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
Ecl136II GAGCTC 2 cut(s) 36, 613
Eco130I CCWWGG 2 cut(s) 642, 673
Eco24I GRGCYC 2 cut(s) 38, 615
Eco53kI GAGCTC 2 cut(s) 36, 613
Eco57I CTGAAG 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 389
EcoICRI GAGCTC 2 cut(s) 36, 613
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 2 cut(s) 642, 673
EcoT38I GRGCYC 2 cut(s) 38, 615
ErhI CCWWGG 2 cut(s) 642, 673
FaeI CATG 2 cut(s) 190, 499
FaiI YATR 9 cut(s) 132, 151, 188, 198, 230, 236, 427, 429, 497
FatI CATG 2 cut(s) 186, 495
FauI CCCGC 1 cut(s) 665
FauNDI CATATG 1 cut(s) 427
Fnu4HI GCNGC 4 cut(s) 23, 53, 369, 530
FokI GGATG 5 cut(s) 374, 512, 561, 591, 656
FriOI GRGCYC 2 cut(s) 38, 615
Fsp4HI GCNGC 4 cut(s) 23, 53, 369, 530
FspBI CTAG 1 cut(s) 512
GlaI GCGC 1 cut(s) 185
GluI GCNGC 4 cut(s) 23, 53, 369, 530
GsuI CTGGAG 1 cut(s) 228
HapII CCGG 1 cut(s) 608
HhaI GCGC 1 cut(s) 186
Hin1II CATG 2 cut(s) 190, 499
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HincII GTYRAC 2 cut(s) 112, 493
HindII GTYRAC 2 cut(s) 112, 493
HinfI GANTC 2 cut(s) 210, 271
HpaI GTTAAC 2 cut(s) 112, 493
HpaII CCGG 1 cut(s) 608
HphI GGTGA 2 cut(s) 428, 641
Hpy166II GTNNAC 3 cut(s) 112, 145, 493
Hpy188III TCNNGA 9 cut(s) 98, 137, 245, 268, 275, 389, 512, 608, 616
Hpy8I GTNNAC 3 cut(s) 112, 145, 493
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 1 cut(s) 449
HpyCH4III ACNGT 4 cut(s) 46, 354, 380, 545
HpyCH4IV ACGT 3 cut(s) 108, 114, 128
HpyCH4V TGCA 3 cut(s) 286, 431, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 422
HpyF3I CTNAG 3 cut(s) 180, 443, 483
HpySE526I ACGT 3 cut(s) 108, 114, 128
Hsp92II CATG 2 cut(s) 190, 499
HspAI GCGC 1 cut(s) 184
Kpn2I TCCGGA 1 cut(s) 607
KspAI GTTAAC 2 cut(s) 112, 493
Kzo9I GATC 3 cut(s) 12, 94, 470
LmnI GCTCC 2 cut(s) 295, 610
Lsp1109I GCAGC 3 cut(s) 64, 355, 541
LweI GCATC 2 cut(s) 515, 669
MaeI CTAG 1 cut(s) 512
MaeII ACGT 3 cut(s) 108, 114, 128
MaeIII GTNAC 5 cut(s) 115, 306, 380, 434, 535
MalI GATC 3 cut(s) 14, 96, 472
MboI GATC 3 cut(s) 12, 94, 470
MboII GAAGA 2 cut(s) 256, 655
MflI RGATCY 1 cut(s) 12
MhlI GDGCHC 2 cut(s) 38, 615
MluCI AATT 3 cut(s) 312, 518, 596
MmeI TCCRAC 1 cut(s) 373
MnlI CCTC 6 cut(s) 272, 286, 336, 385, 478, 559
MroI TCCGGA 1 cut(s) 607
MseI TTAA 5 cut(s) 66, 111, 449, 492, 600
MspCI CTTAAG 1 cut(s) 448
MspI CCGG 1 cut(s) 608
MspR9I CCNGG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 402
MwoI GCNNNNNNNGC 2 cut(s) 230, 422
NdeI CATATG 1 cut(s) 427
NdeII GATC 3 cut(s) 12, 94, 470
NlaIII CATG 2 cut(s) 190, 499
NlaIV GGNNCC 2 cut(s) 347, 465
NmuCI GTSAC 4 cut(s) 306, 380, 434, 535
PaeR7I CTCGAG 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfeI GAWTC 2 cut(s) 210, 271
PkrI GCNGC 4 cut(s) 24, 54, 370, 531
Psp124BI GAGCTC 2 cut(s) 38, 615
Psp1406I AACGTT 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 2 cut(s) 347, 465
PsuI RGATCY 1 cut(s) 12
SacI GAGCTC 2 cut(s) 38, 615
SaqAI TTAA 5 cut(s) 66, 111, 449, 492, 600
SatI GCNGC 4 cut(s) 23, 53, 369, 530
Sau3AI GATC 3 cut(s) 12, 94, 470
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 2 cut(s) 38, 615
SfaNI GCATC 2 cut(s) 515, 669
Sfr274I CTCGAG 1 cut(s) 389
SlaI CTCGAG 1 cut(s) 389
SmlI CTYRAG 4 cut(s) 332, 389, 448, 614
SmoI CTYRAG 4 cut(s) 332, 389, 448, 614
Sse9I AATT 3 cut(s) 312, 518, 596
SsiI CCGC 2 cut(s) 23, 658
SspMI CTAG 1 cut(s) 512
SstI GAGCTC 2 cut(s) 38, 615
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 2 cut(s) 642, 673
TaaI ACNGT 4 cut(s) 46, 354, 380, 545
TaiI ACGT 3 cut(s) 111, 117, 131
TaqI TCGA 4 cut(s) 99, 208, 390, 525
TasI AATT 3 cut(s) 312, 518, 596
TauI GCSGC 1 cut(s) 25
TfiI GAWTC 2 cut(s) 210, 271
Tru1I TTAA 5 cut(s) 66, 111, 449, 492, 600
Tru9I TTAA 5 cut(s) 66, 111, 449, 492, 600
TscAI CASTG 2 cut(s) 480, 550
TseFI GTSAC 4 cut(s) 306, 380, 434, 535
TseI GCWGC 3 cut(s) 52, 368, 529
Tsp45I GTSAC 4 cut(s) 306, 380, 434, 535
TspDTI ATGAA 4 cut(s) 203, 378, 511, 609
TspRI CASTG 2 cut(s) 480, 550
Vha464I CTTAAG 1 cut(s) 448
XapI RAATTY 2 cut(s) 518, 596
XbaI TCTAGA 1 cut(s) 511
XhoI CTCGAG 1 cut(s) 389
XspI CTAG 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.