Rw2G007850

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008378
Physical Location & Seq
Forward (+)
7693092 .. 7695061
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G007850.1

Sequence Viewer

Length: 684 bp
ATGGGAAGAGAGGCTCCACAGCAAACGCCAACGGTGGAGCAATTGGTCGCCGTCAATCCCTACAACCCCGATATCCTCCCCGATCTCGAAAGCTACGTCAACGAGCAGGTTTCGTCGCAGACCTACAGTCTCGATGCGAATCTCTGCCTTCTTCGTCTCTATCAGTTTGAGCCGGAGCGTATGAGCACTCAAATTGTTGCTCGTATTTTGGTTAAGGCACTGATGGCAATGCCCGCTCCCGATTTCAGCCTCTGTCTCTTCCTAATTCCAGAAAGAGTGCAAATGGAGGAGCAGTTCAAGACTCTAATTGTCCTATCTCACTATCTAGAGACAGGAAGATTCAGTCAATTCTGGGATGATGCATCTAAGAATCGTCACATACTTGAAGCTGTCCCAGGTTTTGAGCAAGCGGTCCAAGCCTATGCAGTTCATGTTCTTTCCTTGACTTACCAAAAGGTTCCCAGATCTGTGCTTGCTGAGGCAATCAACCTTGAAGGTATATCCTTGGACAAATTCCTTGAGCATCATGTGGCCAATAGCGGTTGGGTTCTTGAAAAAGGTCATGGCAAGGGCCAACTCATTGTCTTACCTCGAAATGAATTTAACCATCCTGAGCTGAAGAAAAGCACAGACGATGGAATTCCATTAGACCATGTGGCTCGAATCTTCCCCATCCTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

227

Amino Acids

25.69

Weight (kDa)

5.35

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CSN8_PSD8_EIF3K PF10075 62 - 187 1.7e-16 CSN8/PSMD8/EIF3K family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 97
AccBSI CCGCTC 1 cut(s) 236
AciI CCGC 3 cut(s) 234, 410, 540
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 3 cut(s) 512, 599, 639
AcuI CTGAAG 1 cut(s) 638
AfiI CCNNNNNNNGG 2 cut(s) 676, 677
AgsI TTSAA 4 cut(s) 298, 386, 494, 554
AhdI GACNNNNNGTC 1 cut(s) 126
AjnI CCWGG 2 cut(s) 394, 675
AloI GAACNNNNNNTCC 2 cut(s) 278, 310
AluBI AGCT 3 cut(s) 93, 389, 616
AluI AGCT 3 cut(s) 93, 389, 616
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 4 cut(s) 134, 161, 260, 323
AlwNI CAGNNNCTG 1 cut(s) 252
AoxI GGCC 2 cut(s) 531, 571
ApoI RAATTY 3 cut(s) 512, 599, 639
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AspS9I GGNCC 2 cut(s) 412, 571
AvaII GGWCC 1 cut(s) 412
BalI TGGCCA 1 cut(s) 533
Bbv12I GWGCWC 1 cut(s) 188
BbvCI CCTCAGC 1 cut(s) 477
BccI CCATC 4 cut(s) 217, 615, 629, 680
BceAI ACGGC 1 cut(s) 35
BciT130I CCWGG 2 cut(s) 396, 677
BcoDI GTCTC 4 cut(s) 134, 161, 260, 323
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 1 cut(s) 124
BfuAI ACCTGC 1 cut(s) 97
BglII AGATCT 1 cut(s) 464
Bme1390I CCNGG 2 cut(s) 396, 677
Bme18I GGWCC 1 cut(s) 412
BmeRI GACNNNNNGTC 1 cut(s) 126
BmgT120I GGNCC 2 cut(s) 412, 571
BmiI GGNNCC 2 cut(s) 15, 459
BmrFI CCNGG 2 cut(s) 396, 677
BmsI GCATC 4 cut(s) 124, 349, 371, 532
Bpu10I CCTNAGC 2 cut(s) 477, 612
BpuEI CTTGAG 1 cut(s) 539
BsaBI GATNNNNATC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 394, 504, 676
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 2 cut(s) 676, 677
Bse3DI GCAATG 1 cut(s) 234
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 2 cut(s) 396, 677
BseDI CCNNGG 3 cut(s) 394, 504, 676
BseGI GGATG 3 cut(s) 361, 607, 672
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 2 cut(s) 676, 677
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 2 cut(s) 468, 603
BseRI GAGGAG 1 cut(s) 302
BshFI GGCC 2 cut(s) 533, 573
BsiHKAI GWGCWC 1 cut(s) 188
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 2 cut(s) 676, 677
BsmAI GTCTC 4 cut(s) 134, 161, 260, 323
BsmBI CGTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 2 cut(s) 533, 573
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 2 cut(s) 82, 464
BspACI CCGC 3 cut(s) 234, 410, 540
BspANI GGCC 2 cut(s) 533, 573
BspCNI CTCAG 2 cut(s) 469, 604
BspLI GGNNCC 2 cut(s) 15, 459
BspMI ACCTGC 1 cut(s) 97
BsrBI CCGCTC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 234
BssECI CCNNGG 3 cut(s) 394, 504, 676
BssMI GATC 2 cut(s) 82, 464
BssT1I CCWWGG 1 cut(s) 504
Bst2UI CCWGG 2 cut(s) 396, 677
Bst4CI ACNGT 2 cut(s) 34, 128
Bst6I CTCTTC 1 cut(s) 263
BstC8I GCNNGC 3 cut(s) 234, 408, 474
BstDEI CTNAG 3 cut(s) 366, 477, 612
BstF5I GGATG 3 cut(s) 361, 607, 672
BstKTI GATC 2 cut(s) 85, 467
BstMAI GTCTC 4 cut(s) 134, 161, 260, 323
BstMBI GATC 2 cut(s) 82, 464
BstMWI GCNNNNNNNGC 3 cut(s) 224, 233, 416
BstNI CCWGG 2 cut(s) 396, 677
BstSCI CCNGG 2 cut(s) 394, 675
BstSFI CTRYAG 1 cut(s) 124
BstX2I RGATCY 1 cut(s) 464
BstYI RGATCY 1 cut(s) 464
BsuRI GGCC 2 cut(s) 533, 573
BtsCI GGATG 3 cut(s) 361, 607, 672
BtsIMutI CAGTG 1 cut(s) 218
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 3 cut(s) 234, 408, 474
CaiI CAGNNNCTG 1 cut(s) 252
Cfr13I GGNCC 2 cut(s) 412, 571
CviAII CATG 4 cut(s) 431, 527, 563, 653
DdeI CTNAG 3 cut(s) 366, 477, 612
DpnI GATC 2 cut(s) 84, 466
DpnII GATC 2 cut(s) 82, 464
DriI GACNNNNNGTC 1 cut(s) 126
EaeI YGGCCR 1 cut(s) 531
Eam1104I CTCTTC 1 cut(s) 263
Eam1105I GACNNNNNGTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 263
Eco130I CCWWGG 1 cut(s) 504
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 638
EcoRI GAATTC 1 cut(s) 639
EcoRII CCWGG 2 cut(s) 394, 675
EcoRV GATATC 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 504
EcoT22I ATGCAT 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 161
FaeI CATG 4 cut(s) 434, 530, 566, 656
FaiI YATR 8 cut(s) 182, 380, 423, 432, 500, 528, 564, 654
FaqI GGGAC 1 cut(s) 377
FatI CATG 4 cut(s) 430, 526, 562, 652
FauI CCCGC 1 cut(s) 241
FokI GGATG 3 cut(s) 368, 594, 659
FspBI CTAG 1 cut(s) 326
HaeIII GGCC 2 cut(s) 533, 573
HapII CCGG 1 cut(s) 173
Hin1II CATG 4 cut(s) 434, 530, 566, 656
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 5 cut(s) 139, 301, 339, 370, 663
HpaII CCGG 1 cut(s) 173
Hpy166II GTNNAC 1 cut(s) 100
Hpy188III TCNNGA 8 cut(s) 86, 131, 239, 269, 298, 326, 551, 611
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 1 cut(s) 118
HpyAV CCTTC 2 cut(s) 158, 488
HpyCH4III ACNGT 2 cut(s) 34, 128
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 3 cut(s) 280, 362, 425
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 233, 416
HpyF3I CTNAG 3 cut(s) 366, 477, 612
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 4 cut(s) 434, 530, 566, 656
Kzo9I GATC 2 cut(s) 82, 464
LmnI GCTCC 5 cut(s) 19, 37, 175, 241, 289
LweI GCATC 4 cut(s) 124, 349, 371, 532
MaeI CTAG 1 cut(s) 326
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 1 cut(s) 374
MalI GATC 2 cut(s) 84, 466
MbiI CCGCTC 1 cut(s) 236
MboI GATC 2 cut(s) 82, 464
MboII GAAGA 6 cut(s) 18, 143, 250, 348, 631, 658
MfeI CAATTG 1 cut(s) 41
MflI RGATCY 1 cut(s) 464
MhlI GDGCHC 1 cut(s) 188
MlsI TGGCCA 1 cut(s) 533
MluCI AATT 8 cut(s) 41, 192, 264, 306, 347, 512, 599, 639
MluNI TGGCCA 1 cut(s) 533
MlyI GAGTC 1 cut(s) 295
MnlI CCTC 6 cut(s) 4, 86, 260, 280, 472, 600
Mox20I TGGCCA 1 cut(s) 533
Mph1103I ATGCAT 1 cut(s) 364
MscI TGGCCA 1 cut(s) 533
MseI TTAA 2 cut(s) 213, 603
Msp20I TGGCCA 1 cut(s) 533
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 2 cut(s) 396, 677
MunI CAATTG 1 cut(s) 41
MvaI CCWGG 2 cut(s) 396, 677
MwoI GCNNNNNNNGC 3 cut(s) 224, 233, 416
NdeII GATC 2 cut(s) 82, 464
NlaIII CATG 4 cut(s) 434, 530, 566, 656
NlaIV GGNNCC 2 cut(s) 15, 459
NmuCI GTSAC 1 cut(s) 374
NsiI ATGCAT 1 cut(s) 364
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 4 cut(s) 139, 339, 370, 663
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Psp6I CCWGG 2 cut(s) 394, 675
PspGI CCWGG 2 cut(s) 394, 675
PspN4I GGNNCC 2 cut(s) 15, 459
PspPI GGNCC 2 cut(s) 412, 571
PstNI CAGNNNCTG 1 cut(s) 252
PsuI RGATCY 1 cut(s) 464
SaqAI TTAA 2 cut(s) 213, 603
Sau3AI GATC 2 cut(s) 82, 464
Sau96I GGNCC 2 cut(s) 412, 571
SchI GAGTC 1 cut(s) 295
ScrFI CCNGG 2 cut(s) 396, 677
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 4 cut(s) 124, 349, 371, 532
SfcI CTRYAG 1 cut(s) 124
SinI GGWCC 1 cut(s) 412
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
Sse9I AATT 8 cut(s) 41, 192, 264, 306, 347, 512, 599, 639
SsiI CCGC 3 cut(s) 234, 410, 540
SspMI CTAG 1 cut(s) 326
StyD4I CCNGG 2 cut(s) 394, 675
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 34, 128
TaiI ACGT 1 cut(s) 99
TaqI TCGA 4 cut(s) 87, 132, 592, 661
TasI AATT 8 cut(s) 41, 192, 264, 306, 347, 512, 599, 639
TfiI GAWTC 4 cut(s) 139, 339, 370, 663
Tru1I TTAA 2 cut(s) 213, 603
Tru9I TTAA 2 cut(s) 213, 603
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 1 cut(s) 374
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 2 cut(s) 419, 612
TspRI CASTG 1 cut(s) 225
VpaK11BI GGWCC 1 cut(s) 412
XapI RAATTY 3 cut(s) 512, 599, 639
XbaI TCTAGA 1 cut(s) 325
XspI CTAG 1 cut(s) 326
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.