MD02G1102200.v1.1

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008378
Physical Location & Seq
Forward (+)
8200091 .. 8202587
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1102200.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGGAAGAGAGGTTCCACAGCAACAGCAGCAGCAGCAGGTGGCGCTCGCTGTGGAGCAGCTGCTCGCCGTGAATCCGTACAACCCTGACATCCTTCCCGATCTCGAAAACTACGTCAACGAGCAGGTTTCTTCGCAAACCTACAGTCTCGATGCCAATCTCTGCCTTCTTCGCCTCTATCAGTTTGAGCCGGACCGTATGAGCACCCAAATTGTTGCCCGCATCTTGGTTAAGGCATTGATGGCAATGCCCTCTCCTGATTTCAGCCTCTGCCTCTTCTTAATTCCCGAAAGAGTGCAAATGGAGGAGCAGTTCAAGACGCTAATTGTTCTATCTCACTACTTGGAGACTGGACGATTCAGTCAATTCTGGGATGAAGCATCCAAGAATCGTCACATAGTTGAAGCTGTTCCAGGTTTTGAGCAAGCAATCCAAGACTATGCAGTTCATGTTCTTTCCTTGACATACCAGAGGGTTCCCAGATCTGTGCTTGCTGAGGCTATCAACATCGAAGGTTTAGCCCTGGACAAGTTCCTTGAGCACCATGTAGCTAATAGTGGTTGGATTCTTGAGAAAGGTCAAGGCAGGGGTCAACTCATTGTTTTACCTCGAAATGAATTTAACCATCCTGAGCTGAAGAAAAACACTGACGATGGCATCCCATTAGAGCACGTGGCTCGCATCTTCCCCATTCTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

234

Amino Acids

26.6

Weight (kDa)

5.15

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CSN8_PSD8_EIF3K PF10075 68 - 194 1.4e-17 CSN8/PSMD8/EIF3K family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 28
AasI GACNNNNNNGTC 1 cut(s) 360
Acc36I ACCTGC 2 cut(s) 28, 115
AciI CCGC 1 cut(s) 220
AcsI RAATTY 1 cut(s) 617
AcuI CTGAAG 1 cut(s) 656
AcvI CACGTG 1 cut(s) 673
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 2 cut(s) 226, 695
AgsI TTSAA 2 cut(s) 316, 404
AjnI CCWGG 2 cut(s) 412, 522
AloI GAACNNNNNNTCC 3 cut(s) 29, 296, 328
AluBI AGCT 4 cut(s) 61, 407, 551, 634
AluI AGCT 4 cut(s) 61, 407, 551, 634
Alw21I GWGCWC 3 cut(s) 206, 543, 672
Alw26I GTCTC 2 cut(s) 152, 341
AlwNI CAGNNNCTG 1 cut(s) 270
ApeKI GCWGC 5 cut(s) 28, 31, 34, 58, 61
ApoI RAATTY 1 cut(s) 617
ArsI GACNNNNNNTTYG 4 cut(s) 99, 130, 131, 162
Asp700I GAANNNNTTC 1 cut(s) 408
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 1 cut(s) 193
AvaII GGWCC 1 cut(s) 193
BbrPI CACGTG 1 cut(s) 673
Bbv12I GWGCWC 3 cut(s) 206, 543, 672
BbvCI CCTCAGC 1 cut(s) 495
BbvI GCAGC 5 cut(s) 40, 43, 46, 48, 70
BccI CCATC 3 cut(s) 235, 633, 647
BceAI ACGGC 1 cut(s) 53
BcgI CGANNNNNNTGC 2 cut(s) 659, 693
BciT130I CCWGG 2 cut(s) 414, 524
BcoDI GTCTC 2 cut(s) 152, 341
BfmI CTRYAG 1 cut(s) 142
BfoI RGCGCY 1 cut(s) 47
BfuAI ACCTGC 2 cut(s) 28, 115
BglII AGATCT 1 cut(s) 482
BisI GCNGC 5 cut(s) 29, 32, 35, 59, 62
BlsI GCNGC 5 cut(s) 30, 33, 36, 60, 63
Bme1390I CCNGG 2 cut(s) 414, 524
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 2 cut(s) 15, 477
BmrFI CCNGG 2 cut(s) 414, 524
BmsI GCATC 5 cut(s) 142, 231, 389, 666, 690
Bpu10I CCTNAGC 2 cut(s) 495, 630
BpuEI CTTGAG 2 cut(s) 557, 590
BsaAI YACGTR 1 cut(s) 673
BsaBI GATNNNNATC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 522
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 695
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 252
Bse8I GATNNNNATC 1 cut(s) 156
BseBI CCWGG 2 cut(s) 414, 524
BseDI CCNNGG 1 cut(s) 522
BseGI GGATG 5 cut(s) 90, 379, 380, 625, 657
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 2 cut(s) 226, 695
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 2 cut(s) 486, 621
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 5 cut(s) 40, 43, 46, 48, 70
BsiHKAI GWGCWC 3 cut(s) 206, 543, 672
BsiSI CCGG 1 cut(s) 191
BslI CCNNNNNNNGG 2 cut(s) 226, 695
BsmAI GTCTC 2 cut(s) 152, 341
Bsp1286I GDGCHC 3 cut(s) 206, 543, 672
Bsp143I GATC 2 cut(s) 100, 482
BspACI CCGC 1 cut(s) 220
BspCNI CTCAG 2 cut(s) 487, 622
BspLI GGNNCC 2 cut(s) 15, 477
BspMI ACCTGC 2 cut(s) 28, 115
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 1 cut(s) 522
BssMI GATC 2 cut(s) 100, 482
Bst2UI CCWGG 2 cut(s) 414, 524
Bst4CI ACNGT 2 cut(s) 146, 197
Bst6I CTCTTC 1 cut(s) 281
BstBAI YACGTR 1 cut(s) 673
BstC8I GCNNGC 6 cut(s) 48, 66, 220, 426, 492, 679
BstDEI CTNAG 2 cut(s) 495, 630
BstF5I GGATG 5 cut(s) 90, 379, 380, 625, 657
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 2 cut(s) 103, 485
BstMAI GTCTC 2 cut(s) 152, 341
BstMBI GATC 2 cut(s) 100, 482
BstMWI GCNNNNNNNGC 5 cut(s) 28, 34, 43, 171, 242
BstNI CCWGG 2 cut(s) 414, 524
BstSCI CCNGG 2 cut(s) 412, 522
BstSFI CTRYAG 1 cut(s) 142
BstV1I GCAGC 5 cut(s) 40, 43, 46, 48, 70
BstX2I RGATCY 1 cut(s) 482
BstYI RGATCY 1 cut(s) 482
BtsCI GGATG 5 cut(s) 90, 379, 380, 625, 657
BtsIMutI CAGTG 1 cut(s) 645
BveI ACCTGC 2 cut(s) 28, 115
Cac8I GCNNGC 6 cut(s) 48, 66, 220, 426, 492, 679
CaiI CAGNNNCTG 1 cut(s) 270
CfoI GCGC 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 193
CpoI CGGWCCG 1 cut(s) 193
CseI GACGC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 79
CspI CGGWCCG 1 cut(s) 193
CviAII CATG 2 cut(s) 449, 545
CviJI RGCY 9 cut(s) 61, 190, 267, 407, 500, 521, 551, 634, 677
CviKI_1 RGCY 9 cut(s) 61, 190, 267, 407, 500, 521, 551, 634, 677
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 2 cut(s) 495, 630
DpnI GATC 2 cut(s) 102, 484
DpnII GATC 2 cut(s) 100, 482
DrdI GACNNNNNNGTC 1 cut(s) 360
DseDI GACNNNNNNGTC 1 cut(s) 360
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 656
Eco72I CACGTG 1 cut(s) 673
EcoRII CCWGG 2 cut(s) 412, 522
FaeI CATG 2 cut(s) 452, 548
FaiI YATR 6 cut(s) 200, 398, 441, 450, 466, 546
FatI CATG 2 cut(s) 448, 544
FauI CCCGC 1 cut(s) 227
Fnu4HI GCNGC 5 cut(s) 29, 32, 35, 59, 62
FokI GGATG 5 cut(s) 77, 367, 386, 612, 644
Fsp4HI GCNGC 5 cut(s) 29, 32, 35, 59, 62
GlaI GCGC 1 cut(s) 45
GluI GCNGC 5 cut(s) 29, 32, 35, 59, 62
HaeII RGCGCY 1 cut(s) 47
HapII CCGG 1 cut(s) 191
HgaI GACGC 1 cut(s) 328
HhaI GCGC 1 cut(s) 46
Hin1II CATG 2 cut(s) 452, 548
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HincII GTYRAC 2 cut(s) 118, 593
HindII GTYRAC 2 cut(s) 118, 593
HinfI GANTC 4 cut(s) 73, 357, 388, 565
HpaII CCGG 1 cut(s) 191
Hpy166II GTNNAC 2 cut(s) 118, 593
Hpy188III TCNNGA 8 cut(s) 98, 104, 149, 257, 287, 316, 569, 629
Hpy8I GTNNAC 2 cut(s) 118, 593
HpyAV CCTTC 3 cut(s) 104, 176, 506
HpyCH4III ACNGT 2 cut(s) 146, 197
HpyCH4IV ACGT 2 cut(s) 114, 672
HpyCH4V TGCA 2 cut(s) 298, 443
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 34, 43, 171, 242
HpyF3I CTNAG 2 cut(s) 495, 630
HpySE526I ACGT 2 cut(s) 114, 672
Hsp92II CATG 2 cut(s) 452, 548
HspAI GCGC 1 cut(s) 44
Kzo9I GATC 2 cut(s) 100, 482
LmnI GCTCC 2 cut(s) 55, 307
Lsp1109I GCAGC 5 cut(s) 40, 43, 46, 48, 70
LweI GCATC 5 cut(s) 142, 231, 389, 666, 690
MaeII ACGT 2 cut(s) 114, 672
MaeIII GTNAC 1 cut(s) 392
MalI GATC 2 cut(s) 102, 484
MboI GATC 2 cut(s) 100, 482
MboII GAAGA 6 cut(s) 18, 123, 161, 268, 649, 676
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 3 cut(s) 206, 543, 672
MluCI AATT 5 cut(s) 210, 282, 324, 365, 617
MmeI TCCRAC 1 cut(s) 542
MnlI CCTC 9 cut(s) 4, 185, 262, 278, 284, 298, 465, 490, 618
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 3 cut(s) 231, 281, 621
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 1 cut(s) 191
MspR9I CCNGG 2 cut(s) 414, 524
MvaI CCWGG 2 cut(s) 414, 524
MwoI GCNNNNNNNGC 5 cut(s) 28, 34, 43, 171, 242
NdeII GATC 2 cut(s) 100, 482
NlaIII CATG 2 cut(s) 452, 548
NlaIV GGNNCC 2 cut(s) 15, 477
NmuCI GTSAC 1 cut(s) 392
PaqCI CACCTGC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 4 cut(s) 73, 357, 388, 565
PkrI GCNGC 5 cut(s) 30, 33, 36, 60, 63
PmaCI CACGTG 1 cut(s) 673
PmlI CACGTG 1 cut(s) 673
Ppu21I YACGTR 1 cut(s) 673
Psp6I CCWGG 2 cut(s) 412, 522
PspCI CACGTG 1 cut(s) 673
PspGI CCWGG 2 cut(s) 412, 522
PspN4I GGNNCC 2 cut(s) 15, 477
PspPI GGNCC 1 cut(s) 193
PstNI CAGNNNCTG 1 cut(s) 270
PsuI RGATCY 1 cut(s) 482
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
Rsr2I CGGWCCG 1 cut(s) 193
RsrII CGGWCCG 1 cut(s) 193
SaqAI TTAA 3 cut(s) 231, 281, 621
SatI GCNGC 5 cut(s) 29, 32, 35, 59, 62
Sau3AI GATC 2 cut(s) 100, 482
Sau96I GGNCC 1 cut(s) 193
ScrFI CCNGG 2 cut(s) 414, 524
SduI GDGCHC 3 cut(s) 206, 543, 672
SfaNI GCATC 5 cut(s) 142, 231, 389, 666, 690
SfcI CTRYAG 1 cut(s) 142
SinI GGWCC 1 cut(s) 193
SmlI CTYRAG 2 cut(s) 536, 569
SmoI CTYRAG 2 cut(s) 536, 569
Sse9I AATT 5 cut(s) 210, 282, 324, 365, 617
SsiI CCGC 1 cut(s) 220
StyD4I CCNGG 2 cut(s) 412, 522
TaaI ACNGT 2 cut(s) 146, 197
TaiI ACGT 2 cut(s) 117, 675
TaqI TCGA 4 cut(s) 105, 150, 510, 610
TasI AATT 5 cut(s) 210, 282, 324, 365, 617
TfiI GAWTC 4 cut(s) 73, 357, 388, 565
Tru1I TTAA 3 cut(s) 231, 281, 621
Tru9I TTAA 3 cut(s) 231, 281, 621
TscAI CASTG 1 cut(s) 652
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 5 cut(s) 28, 31, 34, 58, 61
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 3 cut(s) 390, 437, 630
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 1 cut(s) 652
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 1 cut(s) 617
XmnI GAANNNNTTC 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.