MD15G1223700.v1.1

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008378
Physical Location & Seq
Forward (+)
18177910 .. 18180275
2366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1223700.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGGAAGAGAGGTTCTGCAGCAACAGCAGCAGCAGCAGGTGGCGTCCGCGGTGGAGGAGCTGCTCGCCGTGAATCCGTACAACCCCGACATCCTTCCCGATCTCGAAAACTACGTCAACGAGCAGGTTTCTTCACAAACCTACAGTCTAGATGCCAATCTCTGCCTTCTTCGCCTCTATCAGTTTGAGCCAAATCGTATGAGCACCCAAATTGTGTCCCGCATCTTGGTCAAGGCACTGATGGCAATGCCGTCTCCTGATTTCAGCCTTTGTCTCTTCTTAATTCCAGAAAGAGTGCAAATGGAGGAGCAGTTCAAGACACTAATTGTTCTATCTCACTACTTGGAGACTGGACGATTCAGTCAATTCTGGGATGAAGCATCCAAGAATCGTCACATAGTTGAAGCTGTTCCAGGTTTTGAGCAAGCAATCCAAGCCTATGCAATTCATGTTCTTTCCTTGACTTATCAAAGGGTACCCAAATCTGTGCTTGCTGAGGCTATCAACATCGAAGGTTTAGCCCTGGACAAGTTCCTTGAGCACCATGTAGCTAATAGCGGTTGGATTCTTGAGAAAGTTCAAGGCAGGGGTCAACTCATTGTCCTACCTCGAAGTGAATTTAACCATCCTGAGCTGAAGAAAAACACTGACGATGGCATTCCATTAGATCACGTGGCTCGCATCTTCCCCATTCTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

234

Amino Acids

26.55

Weight (kDa)

5.25

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CSN8_PSD8_EIF3K PF10075 68 - 192 9e-18 CSN8/PSMD8/EIF3K family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 28
AasI GACNNNNNNGTC 1 cut(s) 360
Acc36I ACCTGC 2 cut(s) 28, 115
Acc65I GGTACC 1 cut(s) 475
AccB1I GGYRCC 1 cut(s) 475
AccII CGCG 1 cut(s) 50
AciI CCGC 4 cut(s) 48, 50, 220, 558
AcsI RAATTY 1 cut(s) 617
AcuI CTGAAG 1 cut(s) 656
AcvI CACGTG 1 cut(s) 673
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 2 cut(s) 80, 477
AfiI CCNNNNNNNGG 2 cut(s) 226, 695
AgsI TTSAA 3 cut(s) 316, 404, 581
AjnI CCWGG 2 cut(s) 412, 522
AloI GAACNNNNNNTCC 3 cut(s) 29, 296, 328
AluBI AGCT 4 cut(s) 61, 407, 551, 634
AluI AGCT 4 cut(s) 61, 407, 551, 634
Alw21I GWGCWC 2 cut(s) 206, 543
Alw26I GTCTC 3 cut(s) 258, 278, 341
ApeKI GCWGC 5 cut(s) 19, 28, 31, 34, 61
ApoI RAATTY 1 cut(s) 617
ArsI GACNNNNNNTTYG 4 cut(s) 99, 130, 131, 162
Asp700I GAANNNNTTC 1 cut(s) 408
Asp718I GGTACC 1 cut(s) 475
BanI GGYRCC 1 cut(s) 475
BbrPI CACGTG 1 cut(s) 673
Bbv12I GWGCWC 2 cut(s) 206, 543
BbvCI CCTCAGC 1 cut(s) 495
BbvI GCAGC 5 cut(s) 31, 40, 43, 46, 48
BccI CCATC 3 cut(s) 235, 633, 647
BceAI ACGGC 2 cut(s) 53, 235
BciT130I CCWGG 2 cut(s) 414, 524
BcoDI GTCTC 3 cut(s) 258, 278, 341
BfaI CTAG 1 cut(s) 149
BfmI CTRYAG 2 cut(s) 17, 142
BfuAI ACCTGC 2 cut(s) 28, 115
BisI GCNGC 5 cut(s) 20, 29, 32, 35, 62
BlsI GCNGC 5 cut(s) 21, 30, 33, 36, 63
Bme1390I CCNGG 2 cut(s) 414, 524
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 2 cut(s) 414, 524
BmsI GCATC 4 cut(s) 142, 231, 389, 690
Bpu10I CCTNAGC 2 cut(s) 495, 630
BpuEI CTTGAG 2 cut(s) 557, 590
BsaAI YACGTR 1 cut(s) 673
BsaBI GATNNNNATC 1 cut(s) 156
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 48, 522
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 695
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 252
Bse8I GATNNNNATC 1 cut(s) 156
BseBI CCWGG 2 cut(s) 414, 524
BseDI CCNNGG 2 cut(s) 48, 522
BseGI GGATG 4 cut(s) 90, 379, 380, 625
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 2 cut(s) 226, 695
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 2 cut(s) 486, 621
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 2 cut(s) 71, 320
BseXI GCAGC 5 cut(s) 31, 40, 43, 46, 48
Bsh1236I CGCG 1 cut(s) 50
BshNI GGYRCC 1 cut(s) 475
BsiHKAI GWGCWC 2 cut(s) 206, 543
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 2 cut(s) 226, 695
BsmAI GTCTC 3 cut(s) 258, 278, 341
BsmBI CGTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 657
Bsp1286I GDGCHC 2 cut(s) 206, 543
Bsp143I GATC 2 cut(s) 100, 667
BspACI CCGC 4 cut(s) 48, 50, 220, 558
BspCNI CTCAG 2 cut(s) 487, 622
BspFNI CGCG 1 cut(s) 50
BspLI GGNNCC 1 cut(s) 477
BspMAI CTGCAG 1 cut(s) 21
BspMI ACCTGC 2 cut(s) 28, 115
BspT107I GGYRCC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 2 cut(s) 48, 522
BssMI GATC 2 cut(s) 100, 667
BssNI GRCGYC 1 cut(s) 44
Bst2UI CCWGG 2 cut(s) 414, 524
Bst4CI ACNGT 1 cut(s) 146
Bst6I CTCTTC 1 cut(s) 281
BstACI GRCGYC 1 cut(s) 44
BstBAI YACGTR 1 cut(s) 673
BstC8I GCNNGC 4 cut(s) 66, 426, 492, 679
BstDEI CTNAG 2 cut(s) 495, 630
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 4 cut(s) 90, 379, 380, 625
BstFNI CGCG 1 cut(s) 50
BstKTI GATC 2 cut(s) 103, 670
BstMAI GTCTC 3 cut(s) 258, 278, 341
BstMBI GATC 2 cut(s) 100, 667
BstMWI GCNNNNNNNGC 6 cut(s) 25, 28, 34, 171, 242, 434
BstNI CCWGG 2 cut(s) 414, 524
BstSCI CCNGG 2 cut(s) 412, 522
BstSFI CTRYAG 2 cut(s) 17, 142
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 5 cut(s) 31, 40, 43, 46, 48
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 4 cut(s) 90, 379, 380, 625
BtsIMutI CAGTG 2 cut(s) 236, 645
BveI ACCTGC 2 cut(s) 28, 115
Cac8I GCNNGC 4 cut(s) 66, 426, 492, 679
Cfr42I CCGCGG 1 cut(s) 51
CseI GACGC 1 cut(s) 33
Csp6I GTAC 2 cut(s) 79, 476
CviAII CATG 2 cut(s) 449, 545
CviQI GTAC 2 cut(s) 79, 476
DdeI CTNAG 2 cut(s) 495, 630
DpnI GATC 2 cut(s) 102, 669
DpnII GATC 2 cut(s) 100, 667
DrdI GACNNNNNNGTC 1 cut(s) 360
DseDI GACNNNNNNGTC 1 cut(s) 360
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 656
Eco72I CACGTG 1 cut(s) 673
EcoRII CCWGG 2 cut(s) 412, 522
Esp3I CGTCTC 1 cut(s) 258
FaeI CATG 2 cut(s) 452, 548
FaiI YATR 5 cut(s) 200, 398, 441, 450, 546
FaqI GGGAC 1 cut(s) 202
FatI CATG 2 cut(s) 448, 544
FauI CCCGC 1 cut(s) 227
Fnu4HI GCNGC 5 cut(s) 20, 29, 32, 35, 62
FokI GGATG 4 cut(s) 77, 367, 386, 612
Fsp4HI GCNGC 5 cut(s) 20, 29, 32, 35, 62
FspBI CTAG 1 cut(s) 149
GluI GCNGC 5 cut(s) 20, 29, 32, 35, 62
HgaI GACGC 1 cut(s) 33
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 2 cut(s) 452, 548
HincII GTYRAC 2 cut(s) 118, 593
HindII GTYRAC 2 cut(s) 118, 593
HinfI GANTC 4 cut(s) 73, 357, 388, 565
Hpy166II GTNNAC 2 cut(s) 118, 593
Hpy188III TCNNGA 8 cut(s) 98, 104, 149, 257, 287, 316, 569, 629
Hpy8I GTNNAC 2 cut(s) 118, 593
HpyAV CCTTC 3 cut(s) 104, 176, 506
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4IV ACGT 2 cut(s) 114, 672
HpyCH4V TGCA 3 cut(s) 19, 298, 443
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 28, 34, 171, 242, 434
HpyF3I CTNAG 2 cut(s) 495, 630
HpySE526I ACGT 2 cut(s) 114, 672
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 2 cut(s) 452, 548
KpnI GGTACC 1 cut(s) 479
KspI CCGCGG 1 cut(s) 51
Kzo9I GATC 2 cut(s) 100, 667
LmnI GCTCC 2 cut(s) 58, 307
Lsp1109I GCAGC 5 cut(s) 31, 40, 43, 46, 48
LweI GCATC 4 cut(s) 142, 231, 389, 690
MaeI CTAG 1 cut(s) 149
MaeII ACGT 2 cut(s) 114, 672
MaeIII GTNAC 1 cut(s) 392
MalI GATC 2 cut(s) 102, 669
MboI GATC 2 cut(s) 100, 667
MboII GAAGA 6 cut(s) 18, 123, 161, 268, 649, 676
MhlI GDGCHC 2 cut(s) 206, 543
MluCI AATT 6 cut(s) 210, 282, 324, 365, 444, 617
MmeI TCCRAC 1 cut(s) 542
MnlI CCTC 6 cut(s) 4, 49, 185, 298, 490, 618
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 2 cut(s) 281, 621
MspA1I CMGCKG 1 cut(s) 50
MspR9I CCNGG 2 cut(s) 414, 524
Mva1269I GAATGC 1 cut(s) 657
MvaI CCWGG 2 cut(s) 414, 524
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 6 cut(s) 25, 28, 34, 171, 242, 434
NdeII GATC 2 cut(s) 100, 667
NlaIII CATG 2 cut(s) 452, 548
NlaIV GGNNCC 1 cut(s) 477
NmuCI GTSAC 1 cut(s) 392
PaqCI CACCTGC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 1 cut(s) 657
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 4 cut(s) 73, 357, 388, 565
PkrI GCNGC 5 cut(s) 21, 30, 33, 36, 63
PmaCI CACGTG 1 cut(s) 673
PmlI CACGTG 1 cut(s) 673
Ppu21I YACGTR 1 cut(s) 673
Psp6I CCWGG 2 cut(s) 412, 522
PspCI CACGTG 1 cut(s) 673
PspGI CCWGG 2 cut(s) 412, 522
PspN4I GGNNCC 1 cut(s) 477
PstI CTGCAG 1 cut(s) 21
RsaI GTAC 2 cut(s) 80, 477
RsaNI GTAC 2 cut(s) 79, 476
SacII CCGCGG 1 cut(s) 51
SaqAI TTAA 2 cut(s) 281, 621
SatI GCNGC 5 cut(s) 20, 29, 32, 35, 62
Sau3AI GATC 2 cut(s) 100, 667
ScrFI CCNGG 2 cut(s) 414, 524
SduI GDGCHC 2 cut(s) 206, 543
SfaNI GCATC 4 cut(s) 142, 231, 389, 690
SfcI CTRYAG 2 cut(s) 17, 142
Sfr303I CCGCGG 1 cut(s) 51
SgrBI CCGCGG 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 536, 569
SmoI CTYRAG 2 cut(s) 536, 569
Sse9I AATT 6 cut(s) 210, 282, 324, 365, 444, 617
SsiI CCGC 4 cut(s) 48, 50, 220, 558
SspMI CTAG 1 cut(s) 149
StyD4I CCNGG 2 cut(s) 412, 522
TaaI ACNGT 1 cut(s) 146
TaiI ACGT 2 cut(s) 117, 675
TaqI TCGA 3 cut(s) 105, 510, 610
TasI AATT 6 cut(s) 210, 282, 324, 365, 444, 617
TfiI GAWTC 4 cut(s) 73, 357, 388, 565
Tru1I TTAA 2 cut(s) 281, 621
Tru9I TTAA 2 cut(s) 281, 621
TscAI CASTG 2 cut(s) 243, 652
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 5 cut(s) 19, 28, 31, 34, 61
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 2 cut(s) 390, 437
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 2 cut(s) 243, 652
XapI RAATTY 1 cut(s) 617
XbaI TCTAGA 1 cut(s) 148
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.