MD02G1101200.v1.1

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008378
Physical Location & Seq
Forward (+)
8103064 .. 8110685
7622 bp
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UTR
Exon/CDS
Intron
MD02G1101200.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGGAAGAGAGGTTCCACATCAACAGGCAGCAGTAGCAGGTGGCGCTCGCCGTGAATCCGTCAAAATCGAGGGTTTTGACCTTACCTTTTCTCTATGCCAAAAACCCGGTTCCGACGACGAACTCCGGCAAGTTCGTAGGTGTGTGTGTGTACAAGTGTGTGTGTGTCCGTGTGTGTGTATGCGAACTGTGGGTGTGTGTGAGCATGCTGTGCATGCCGTGTATACTGTGCGTGTTTCTTCGCAAACCTACAGTCTCGATGCCAATCTCTGCCTTCTTCGCCACTATCAGTGCTTTATTGATGTGCTGGCACTGATGGCAATGCCCTCTCCTGATTTCAGCCTTTGCCTCTTCTTAATTCCTGAAAGAGTGCAAATGGAGGAGCAGTTCAAGACACTAATTGTTCTGTCTCACTACTTGGAGACTGGACGATTCAGTCAATTCTGGGATGAAGCATCCAAGAATCGTCACATAGTTGAAGCTGTACCAGAAATTAAGAAGAAGAGAGAAGGAACTTGCGCATTCAACAAATTGTTTTCCAACTTTGCCACCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

186

Amino Acids

20.94

Weight (kDa)

7.51

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CSN8_PSD8_EIF3K PF10075 103 - 161 6.2e-06 CSN8/PSMD8/EIF3K family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 29
AasI GACNNNNNNGTC 1 cut(s) 435
Acc16I TGCGCA 1 cut(s) 520
Acc36I ACCTGC 1 cut(s) 29
AccI GTMKAC 1 cut(s) 224
AfaI GTAC 2 cut(s) 153, 486
AgsI TTSAA 3 cut(s) 391, 479, 526
AloI GAACNNNNNNTCC 3 cut(s) 29, 371, 403
AluBI AGCT 1 cut(s) 482
AluI AGCT 1 cut(s) 482
Alw26I GTCTC 3 cut(s) 260, 414, 416
ApeKI GCWGC 1 cut(s) 29
ArsI GACNNNNNNTTYG 2 cut(s) 238, 270
AspLEI GCGC 2 cut(s) 47, 521
AsuC2I CCSGG 1 cut(s) 108
BbvI GCAGC 1 cut(s) 41
BccI CCATC 1 cut(s) 310
BceAI ACGGC 2 cut(s) 36, 203
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 3 cut(s) 260, 414, 416
BfmI CTRYAG 1 cut(s) 250
BfoI RGCGCY 1 cut(s) 48
BfuAI ACCTGC 1 cut(s) 29
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 1 cut(s) 108
BmiI GGNNCC 2 cut(s) 15, 112
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 2 cut(s) 250, 464
BpuMI CCSGG 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 264
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bse1I ACTGG 1 cut(s) 430
Bse3DI GCAATG 1 cut(s) 327
Bse8I GATNNNNATC 1 cut(s) 264
BseGI GGATG 2 cut(s) 454, 455
BseJI GATNNNNATC 1 cut(s) 264
BseMI GCAATG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 430
BseRI GAGGAG 1 cut(s) 395
BseXI GCAGC 1 cut(s) 41
BsiSI CCGG 2 cut(s) 108, 127
BsmAI GTCTC 3 cut(s) 260, 414, 416
BsmI GAATGC 1 cut(s) 521
Bsp1407I TGTACA 1 cut(s) 151
BspLI GGNNCC 2 cut(s) 15, 112
BspMI ACCTGC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 327
BsrGI TGTACA 1 cut(s) 151
BsrI ACTGG 1 cut(s) 430
BssNAI GTATAC 1 cut(s) 225
Bst1107I GTATAC 1 cut(s) 225
Bst4CI ACNGT 3 cut(s) 190, 229, 254
Bst6I CTCTTC 2 cut(s) 356, 497
BstAPI GCANNNNNTGC 1 cut(s) 211
BstAUI TGTACA 1 cut(s) 151
BstC8I GCNNGC 4 cut(s) 49, 207, 216, 309
BstF5I GGATG 2 cut(s) 454, 455
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 2 cut(s) 47, 521
BstMAI GTCTC 3 cut(s) 260, 414, 416
BstMWI GCNNNNNNNGC 6 cut(s) 35, 44, 211, 215, 279, 317
BstNSI RCATGY 2 cut(s) 209, 218
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 41
BstZ17I GTATAC 1 cut(s) 225
BtsCI GGATG 2 cut(s) 454, 455
BtsIMutI CAGTG 2 cut(s) 296, 311
BveI ACCTGC 1 cut(s) 29
Cac8I GCNNGC 4 cut(s) 49, 207, 216, 309
CfoI GCGC 2 cut(s) 47, 521
Csp6I GTAC 2 cut(s) 152, 485
CviAII CATG 2 cut(s) 206, 215
CviJI RGCY 2 cut(s) 342, 482
CviKI_1 RGCY 2 cut(s) 342, 482
CviQI GTAC 2 cut(s) 152, 485
DrdI GACNNNNNNGTC 1 cut(s) 435
DseDI GACNNNNNNGTC 1 cut(s) 435
Eam1104I CTCTTC 2 cut(s) 356, 497
EarI CTCTTC 2 cut(s) 356, 497
FaeI CATG 2 cut(s) 209, 218
FaiI YATR 7 cut(s) 97, 182, 207, 216, 225, 473, 556
FatI CATG 2 cut(s) 205, 214
FblI GTMKAC 1 cut(s) 224
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 2 cut(s) 442, 461
Fsp4HI GCNGC 1 cut(s) 30
FspI TGCGCA 1 cut(s) 520
GlaI GCGC 2 cut(s) 46, 520
GluI GCNGC 1 cut(s) 30
HaeII RGCGCY 1 cut(s) 48
HapII CCGG 2 cut(s) 108, 127
HhaI GCGC 2 cut(s) 47, 521
Hin1II CATG 2 cut(s) 209, 218
Hin6I GCGC 2 cut(s) 45, 519
HinP1I GCGC 2 cut(s) 45, 519
HinfI GANTC 3 cut(s) 56, 432, 463
HpaII CCGG 2 cut(s) 108, 127
Hpy166II GTNNAC 2 cut(s) 152, 225
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 4 cut(s) 257, 332, 362, 391
Hpy8I GTNNAC 2 cut(s) 152, 225
Hpy99I CGWCG 2 cut(s) 119, 122
HpyAV CCTTC 2 cut(s) 284, 503
HpyCH4III ACNGT 3 cut(s) 190, 229, 254
HpyCH4V TGCA 2 cut(s) 214, 373
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 44, 211, 215, 279, 317
Hsp92II CATG 2 cut(s) 209, 218
HspAI GCGC 2 cut(s) 45, 519
LmnI GCTCC 1 cut(s) 382
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 2 cut(s) 250, 464
MaeIII GTNAC 1 cut(s) 467
MboII GAAGA 6 cut(s) 18, 231, 269, 343, 511, 514
MluCI AATT 5 cut(s) 357, 399, 440, 492, 530
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 5 cut(s) 4, 64, 337, 359, 373
MseI TTAA 2 cut(s) 356, 495
MspI CCGG 2 cut(s) 108, 127
MspR9I CCNGG 1 cut(s) 108
Mva1269I GAATGC 1 cut(s) 521
MwoI GCNNNNNNNGC 6 cut(s) 35, 44, 211, 215, 279, 317
NciI CCSGG 1 cut(s) 108
NlaIII CATG 2 cut(s) 209, 218
NlaIV GGNNCC 2 cut(s) 15, 112
NmuCI GTSAC 1 cut(s) 467
NsbI TGCGCA 1 cut(s) 520
NspI RCATGY 2 cut(s) 209, 218
PaeI GCATGC 2 cut(s) 209, 218
PaqCI CACCTGC 1 cut(s) 29
PctI GAATGC 1 cut(s) 521
PfeI GAWTC 3 cut(s) 56, 432, 463
PkrI GCNGC 1 cut(s) 31
PspN4I GGNNCC 2 cut(s) 15, 112
RsaI GTAC 2 cut(s) 153, 486
RsaNI GTAC 2 cut(s) 152, 485
SaqAI TTAA 2 cut(s) 356, 495
SatI GCNGC 1 cut(s) 30
ScrFI CCNGG 1 cut(s) 108
SetI ASST 8 cut(s) 15, 43, 84, 89, 143, 251, 484, 554
SfaNI GCATC 2 cut(s) 250, 464
SfcI CTRYAG 1 cut(s) 250
SphI GCATGC 2 cut(s) 209, 218
Sse9I AATT 5 cut(s) 357, 399, 440, 492, 530
StyD4I CCNGG 1 cut(s) 106
TaaI ACNGT 3 cut(s) 190, 229, 254
TaqI TCGA 2 cut(s) 69, 258
TasI AATT 5 cut(s) 357, 399, 440, 492, 530
TatI WGTACW 1 cut(s) 151
TfiI GAWTC 3 cut(s) 56, 432, 463
Tru1I TTAA 2 cut(s) 356, 495
Tru9I TTAA 2 cut(s) 356, 495
TscAI CASTG 2 cut(s) 296, 318
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 1 cut(s) 465
TspGWI ACGGA 2 cut(s) 49, 159
TspRI CASTG 2 cut(s) 296, 318
XceI RCATGY 2 cut(s) 209, 218
XmiI GTMKAC 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.