pycom15g19790

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008378
Physical Location & Seq
Forward (+)
14190363 .. 14192323
1961 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g19790.8

Sequence Viewer

Length: 702 bp
ATGGGAAGAGAGGTTCTGCAGCAACAGCAGCAGCAGCAGGTGGCGTCCGCGGTGGAGGAGCTGCTCGCCGTGAATCCGTACAACCCCGACATCCTTCCCGATCTCGAAAACTACGTCAACGAGCAGGTTTCTTCGCAAACCTACAGTCTCGATGCCAATCTCTGCCTTCTTCGCCTCTATCAGTTTGAGCCAAATCGTATGAGCACCCAAATTGTGTCCCACATCTTGGTCAAGGCACTGATGGCAATGCCGTCTCCTGATTTCAGCCTTTGTCTCTTCTTAATTCCAGAAAGAGTGCAAATGGAGGAGCAGTTCAAGACACTAATTGTTCTATCTCACTATTTGGAGACTGGACAATTCAGTCAATTCTGGGATGAAGCATCCAAGAATCGTCACATAGTTGAAGCTGTTCCAGGTTTTGAGCAAGCAATCCAAGCCTATGCAATTCATGTTCTTTCCTTGACTTATCAAAGGGTACCCAAATCTGTGCTTGCTGAGGCTATCAACATTGAAGGTTTAGCCCTGGACAAGTTCCTTGAGCACCATGTAGCTAATAGCGGTTGGATTCTTGAGAAAGTTCAAGGCAGGGGTCAACTCATTGTCCTACCTCGAAGTGAATTTAACCATCCTGAGCTGAAGAAAAACACTGACGATGGCATTCCATTAGAGCACGTGGCTCGCATCTTCCCCATTCTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

234

Amino Acids

26.52

Weight (kDa)

5.14

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 28
AasI GACNNNNNNGTC 1 cut(s) 360
Acc36I ACCTGC 2 cut(s) 28, 115
Acc65I GGTACC 1 cut(s) 475
AccB1I GGYRCC 1 cut(s) 475
AccB7I CCANNNNNTGG 1 cut(s) 226
AccII CGCG 1 cut(s) 50
AciI CCGC 3 cut(s) 48, 50, 558
AcsI RAATTY 1 cut(s) 617
AcuI CTGAAG 1 cut(s) 656
AcvI CACGTG 1 cut(s) 673
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 2 cut(s) 80, 477
AfiI CCNNNNNNNGG 2 cut(s) 226, 695
AgsI TTSAA 4 cut(s) 316, 404, 512, 581
AjnI CCWGG 2 cut(s) 412, 522
AloI GAACNNNNNNTCC 3 cut(s) 29, 296, 328
AluBI AGCT 4 cut(s) 61, 407, 551, 634
AluI AGCT 4 cut(s) 61, 407, 551, 634
Alw21I GWGCWC 3 cut(s) 206, 543, 672
Alw26I GTCTC 4 cut(s) 152, 258, 278, 341
ApeKI GCWGC 5 cut(s) 19, 28, 31, 34, 61
ApoI RAATTY 1 cut(s) 617
ArsI GACNNNNNNTTYG 4 cut(s) 99, 130, 131, 162
Asp700I GAANNNNTTC 1 cut(s) 408
Asp718I GGTACC 1 cut(s) 475
BanI GGYRCC 1 cut(s) 475
BbrPI CACGTG 1 cut(s) 673
Bbv12I GWGCWC 3 cut(s) 206, 543, 672
BbvCI CCTCAGC 1 cut(s) 495
BbvI GCAGC 5 cut(s) 31, 40, 43, 46, 48
BccI CCATC 3 cut(s) 235, 633, 647
BceAI ACGGC 2 cut(s) 53, 235
BcgI CGANNNNNNTGC 2 cut(s) 659, 693
BciT130I CCWGG 2 cut(s) 414, 524
BcoDI GTCTC 4 cut(s) 152, 258, 278, 341
BfmI CTRYAG 2 cut(s) 17, 142
BfuAI ACCTGC 2 cut(s) 28, 115
BisI GCNGC 5 cut(s) 20, 29, 32, 35, 62
BlsI GCNGC 5 cut(s) 21, 30, 33, 36, 63
Bme1390I CCNGG 2 cut(s) 414, 524
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 2 cut(s) 414, 524
BmsI GCATC 3 cut(s) 142, 389, 690
Bpu10I CCTNAGC 2 cut(s) 495, 630
BpuEI CTTGAG 2 cut(s) 557, 590
BsaAI YACGTR 1 cut(s) 673
BsaBI GATNNNNATC 1 cut(s) 156
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 48, 522
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 695
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 252
Bse8I GATNNNNATC 1 cut(s) 156
BseBI CCWGG 2 cut(s) 414, 524
BseDI CCNNGG 2 cut(s) 48, 522
BseGI GGATG 4 cut(s) 90, 379, 380, 625
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 2 cut(s) 226, 695
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 2 cut(s) 486, 621
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 2 cut(s) 71, 320
BseXI GCAGC 5 cut(s) 31, 40, 43, 46, 48
Bsh1236I CGCG 1 cut(s) 50
BshNI GGYRCC 1 cut(s) 475
BsiHKAI GWGCWC 3 cut(s) 206, 543, 672
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 2 cut(s) 226, 695
BsmAI GTCTC 4 cut(s) 152, 258, 278, 341
BsmBI CGTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 657
Bsp1286I GDGCHC 3 cut(s) 206, 543, 672
Bsp143I GATC 1 cut(s) 100
BspACI CCGC 3 cut(s) 48, 50, 558
BspCNI CTCAG 2 cut(s) 487, 622
BspFNI CGCG 1 cut(s) 50
BspLI GGNNCC 1 cut(s) 477
BspMAI CTGCAG 1 cut(s) 21
BspMI ACCTGC 2 cut(s) 28, 115
BspT107I GGYRCC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 2 cut(s) 48, 522
BssMI GATC 1 cut(s) 100
BssNI GRCGYC 1 cut(s) 44
Bst2UI CCWGG 2 cut(s) 414, 524
Bst4CI ACNGT 1 cut(s) 146
Bst6I CTCTTC 1 cut(s) 281
BstACI GRCGYC 1 cut(s) 44
BstBAI YACGTR 1 cut(s) 673
BstC8I GCNNGC 4 cut(s) 66, 426, 492, 679
BstDEI CTNAG 2 cut(s) 495, 630
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 4 cut(s) 90, 379, 380, 625
BstFNI CGCG 1 cut(s) 50
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 4 cut(s) 152, 258, 278, 341
BstMBI GATC 1 cut(s) 100
BstMWI GCNNNNNNNGC 6 cut(s) 25, 28, 34, 171, 242, 434
BstNI CCWGG 2 cut(s) 414, 524
BstSCI CCNGG 2 cut(s) 412, 522
BstSFI CTRYAG 2 cut(s) 17, 142
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 5 cut(s) 31, 40, 43, 46, 48
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 4 cut(s) 90, 379, 380, 625
BtsIMutI CAGTG 2 cut(s) 236, 645
BveI ACCTGC 2 cut(s) 28, 115
Cac8I GCNNGC 4 cut(s) 66, 426, 492, 679
Cfr42I CCGCGG 1 cut(s) 51
CseI GACGC 1 cut(s) 33
Csp6I GTAC 2 cut(s) 79, 476
CviAII CATG 2 cut(s) 449, 545
CviQI GTAC 2 cut(s) 79, 476
DdeI CTNAG 2 cut(s) 495, 630
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
DrdI GACNNNNNNGTC 1 cut(s) 360
DseDI GACNNNNNNGTC 1 cut(s) 360
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 656
Eco72I CACGTG 1 cut(s) 673
EcoRII CCWGG 2 cut(s) 412, 522
Esp3I CGTCTC 1 cut(s) 258
FaeI CATG 2 cut(s) 452, 548
FaiI YATR 5 cut(s) 200, 398, 441, 450, 546
FaqI GGGAC 1 cut(s) 202
FatI CATG 2 cut(s) 448, 544
Fnu4HI GCNGC 5 cut(s) 20, 29, 32, 35, 62
FokI GGATG 4 cut(s) 77, 367, 386, 612
Fsp4HI GCNGC 5 cut(s) 20, 29, 32, 35, 62
GluI GCNGC 5 cut(s) 20, 29, 32, 35, 62
HgaI GACGC 1 cut(s) 33
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 2 cut(s) 452, 548
HincII GTYRAC 2 cut(s) 118, 593
HindII GTYRAC 2 cut(s) 118, 593
HinfI GANTC 3 cut(s) 73, 388, 565
Hpy166II GTNNAC 2 cut(s) 118, 593
Hpy188III TCNNGA 8 cut(s) 98, 104, 149, 257, 287, 316, 569, 629
Hpy8I GTNNAC 2 cut(s) 118, 593
HpyAV CCTTC 3 cut(s) 104, 176, 506
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4IV ACGT 2 cut(s) 114, 672
HpyCH4V TGCA 3 cut(s) 19, 298, 443
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 28, 34, 171, 242, 434
HpyF3I CTNAG 2 cut(s) 495, 630
HpySE526I ACGT 2 cut(s) 114, 672
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 2 cut(s) 452, 548
KpnI GGTACC 1 cut(s) 479
KspI CCGCGG 1 cut(s) 51
Kzo9I GATC 1 cut(s) 100
LmnI GCTCC 2 cut(s) 58, 307
Lsp1109I GCAGC 5 cut(s) 31, 40, 43, 46, 48
LweI GCATC 3 cut(s) 142, 389, 690
MaeII ACGT 2 cut(s) 114, 672
MaeIII GTNAC 1 cut(s) 392
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MboII GAAGA 6 cut(s) 18, 123, 161, 268, 649, 676
MhlI GDGCHC 3 cut(s) 206, 543, 672
MluCI AATT 7 cut(s) 210, 282, 324, 356, 365, 444, 617
MmeI TCCRAC 1 cut(s) 542
MnlI CCTC 6 cut(s) 4, 49, 185, 298, 490, 618
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 2 cut(s) 281, 621
MspA1I CMGCKG 1 cut(s) 50
MspR9I CCNGG 2 cut(s) 414, 524
Mva1269I GAATGC 1 cut(s) 657
MvaI CCWGG 2 cut(s) 414, 524
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 6 cut(s) 25, 28, 34, 171, 242, 434
NdeII GATC 1 cut(s) 100
NlaIII CATG 2 cut(s) 452, 548
NlaIV GGNNCC 1 cut(s) 477
NmuCI GTSAC 1 cut(s) 392
PaqCI CACCTGC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 1 cut(s) 657
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 3 cut(s) 73, 388, 565
PflMI CCANNNNNTGG 1 cut(s) 226
PkrI GCNGC 5 cut(s) 21, 30, 33, 36, 63
PmaCI CACGTG 1 cut(s) 673
PmlI CACGTG 1 cut(s) 673
Ppu21I YACGTR 1 cut(s) 673
Psp6I CCWGG 2 cut(s) 412, 522
PspCI CACGTG 1 cut(s) 673
PspGI CCWGG 2 cut(s) 412, 522
PspN4I GGNNCC 1 cut(s) 477
PstI CTGCAG 1 cut(s) 21
RsaI GTAC 2 cut(s) 80, 477
RsaNI GTAC 2 cut(s) 79, 476
SacII CCGCGG 1 cut(s) 51
SaqAI TTAA 2 cut(s) 281, 621
SatI GCNGC 5 cut(s) 20, 29, 32, 35, 62
Sau3AI GATC 1 cut(s) 100
ScrFI CCNGG 2 cut(s) 414, 524
SduI GDGCHC 3 cut(s) 206, 543, 672
SfaNI GCATC 3 cut(s) 142, 389, 690
SfcI CTRYAG 2 cut(s) 17, 142
Sfr303I CCGCGG 1 cut(s) 51
SgrBI CCGCGG 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 536, 569
SmoI CTYRAG 2 cut(s) 536, 569
Sse9I AATT 7 cut(s) 210, 282, 324, 356, 365, 444, 617
SsiI CCGC 3 cut(s) 48, 50, 558
StyD4I CCNGG 2 cut(s) 412, 522
TaaI ACNGT 1 cut(s) 146
TaiI ACGT 2 cut(s) 117, 675
TaqI TCGA 3 cut(s) 105, 150, 610
TasI AATT 7 cut(s) 210, 282, 324, 356, 365, 444, 617
TfiI GAWTC 3 cut(s) 73, 388, 565
Tru1I TTAA 2 cut(s) 281, 621
Tru9I TTAA 2 cut(s) 281, 621
TscAI CASTG 2 cut(s) 243, 652
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 5 cut(s) 19, 28, 31, 34, 61
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 2 cut(s) 390, 437
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 2 cut(s) 243, 652
Van91I CCANNNNNTGG 1 cut(s) 226
XapI RAATTY 1 cut(s) 617
XmnI GAANNNNTTC 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.