RLG00000009224

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008378
Physical Location & Seq
Forward (+)
49339231 .. 49340934
1704 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009224

Sequence Viewer

Length: 456 bp
ATGATAAAAGTCACCTGTTGTTTATGCTTGTTAGCCTACGTTTGTTTTGTATGTGCTCTTGAACATCGCATTATATTGACAGCTGGTGGATCTTACTTTCAGGGCTTAACAATTGTTACTCATGTAACTAAAGAAGGTGGTAAATTTATTGCGAAGATATTCCGTGGAAAAGATACTAGTCTTCTTTATTGTCAGCTGAAAGTATTTTTCCCCATTGTGACTCTTGCAAAACCAAAAAGCAGCCGCAATGCCAGCATAGATTCATCTTGCTTCATTACCTGGACATTAGTACTACTTCAACTCATGACGAGCGTCTCTCTCCACCCAAAATTAAGTGAATGTTGGTATCCTGACTCTGGGGCTCTAAGGTCAAGAATCCCACAGCTCACATTGAACACGAAGCCAGTACCTCCAAAATTGGTCAAGACCAGCGTTACTTTCAGCATAGGGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

152

Amino Acids

16.7

Weight (kDa)

9.41

Isoelectric Point (pI)

28.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 38 - 85 2e-06 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 244
AclWI GGATC 1 cut(s) 97
AcsI RAATTY 1 cut(s) 143
AfaI GTAC 2 cut(s) 291, 408
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 3 cut(s) 62, 299, 394
AhlI ACTAGT 1 cut(s) 176
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 3 cut(s) 83, 196, 385
AluI AGCT 3 cut(s) 83, 196, 385
Alw21I GWGCWC 1 cut(s) 58
Alw26I GTCTC 1 cut(s) 319
AlwI GGATC 1 cut(s) 97
ApeKI GCWGC 1 cut(s) 240
ApoI RAATTY 1 cut(s) 143
Asp700I GAANNNNTTC 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 4
BanII GRGCYC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 58
BbvI GCAGC 1 cut(s) 252
BciT130I CCWGG 1 cut(s) 280
BciVI GTATCC 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 319
BcuI ACTAGT 1 cut(s) 176
BfaI CTAG 1 cut(s) 177
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 2 cut(s) 241, 244
BlsI GCNGC 2 cut(s) 242, 245
BmcAI AGTACT 1 cut(s) 291
Bme1390I CCNGG 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 280
BoxI GACNNNNGTC 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 173
BplI GAGNNNNNCTC 2 cut(s) 301, 333
BsaJI CCNNGG 1 cut(s) 163
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse1I ACTGG 1 cut(s) 404
Bse3DI GCAATG 1 cut(s) 253
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 1 cut(s) 163
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMI GCAATG 1 cut(s) 253
BseNI ACTGG 1 cut(s) 404
BseXI GCAGC 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 58
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 319
BsmBI CGTCTC 1 cut(s) 319
Bsp1286I GDGCHC 2 cut(s) 58, 364
Bsp143I GATC 1 cut(s) 89
BspACI CCGC 1 cut(s) 244
BspHI TCATGA 1 cut(s) 303
BspPI GGATC 1 cut(s) 97
BsrDI GCAATG 1 cut(s) 253
BsrI ACTGG 1 cut(s) 404
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 1 cut(s) 365
BstDSI CCRYGG 1 cut(s) 163
BstKTI GATC 1 cut(s) 92
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 1 cut(s) 89
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstNI CCWGG 1 cut(s) 280
BstPAI GACNNNNGTC 1 cut(s) 311
BstSCI CCNGG 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 1 cut(s) 89
BstYI RGATCY 1 cut(s) 89
BsuI GTATCC 1 cut(s) 357
BtgI CCRYGG 1 cut(s) 163
BtgZI GCGATG 1 cut(s) 50
Cac8I GCNNGC 1 cut(s) 253
CciI TCATGA 1 cut(s) 303
CseI GACGC 1 cut(s) 301
Csp6I GTAC 2 cut(s) 290, 407
CviAII CATG 2 cut(s) 122, 304
CviJI RGCY 8 cut(s) 35, 83, 105, 196, 243, 362, 385, 403
CviKI_1 RGCY 8 cut(s) 35, 83, 105, 196, 243, 362, 385, 403
CviQI GTAC 2 cut(s) 290, 407
DdeI CTNAG 1 cut(s) 365
DpnI GATC 1 cut(s) 91
DpnII GATC 1 cut(s) 89
Eco24I GRGCYC 1 cut(s) 364
EcoRII CCWGG 1 cut(s) 278
EcoT38I GRGCYC 1 cut(s) 364
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 2 cut(s) 125, 307
FaiI YATR 7 cut(s) 25, 52, 74, 123, 257, 305, 446
FatI CATG 2 cut(s) 121, 303
Fnu4HI GCNGC 2 cut(s) 241, 244
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 2 cut(s) 241, 244
FspBI CTAG 1 cut(s) 177
GluI GCNGC 2 cut(s) 241, 244
HgaI GACGC 1 cut(s) 301
Hin1II CATG 2 cut(s) 125, 307
HinfI GANTC 4 cut(s) 220, 260, 353, 375
HphI GGTGA 1 cut(s) 4
Hpy188III TCNNGA 5 cut(s) 59, 304, 350, 372, 424
HpyAV CCTTC 1 cut(s) 128
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 1 cut(s) 227
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
HpyF3I CTNAG 1 cut(s) 365
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 2 cut(s) 125, 307
Kzo9I GATC 1 cut(s) 89
Lsp1109I GCAGC 1 cut(s) 252
MaeI CTAG 1 cut(s) 177
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 5 cut(s) 10, 115, 124, 217, 433
MalI GATC 1 cut(s) 91
MboI GATC 1 cut(s) 89
MboII GAAGA 2 cut(s) 166, 173
MfeI CAATTG 1 cut(s) 111
MflI RGATCY 1 cut(s) 89
MhlI GDGCHC 2 cut(s) 58, 364
MluCI AATT 4 cut(s) 111, 143, 329, 416
MlyI GAGTC 2 cut(s) 214, 347
MnlI CCTC 1 cut(s) 420
MroXI GAANNNNTTC 1 cut(s) 158
MseI TTAA 2 cut(s) 107, 332
MspA1I CMGCKG 2 cut(s) 83, 196
MspR9I CCNGG 1 cut(s) 280
MunI CAATTG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 280
MwoI GCNNNNNNNGC 1 cut(s) 252
NdeII GATC 1 cut(s) 89
NlaIII CATG 2 cut(s) 125, 307
NmuCI GTSAC 2 cut(s) 10, 217
PagI TCATGA 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 2 cut(s) 260, 375
PkrI GCNGC 2 cut(s) 242, 245
PleI GAGTC 2 cut(s) 214, 347
PpsI GAGTC 2 cut(s) 214, 347
PshAI GACNNNNGTC 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 89
PvuII CAGCTG 2 cut(s) 83, 196
RsaI GTAC 2 cut(s) 291, 408
RsaNI GTAC 2 cut(s) 290, 407
SaqAI TTAA 2 cut(s) 107, 332
SatI GCNGC 2 cut(s) 241, 244
Sau3AI GATC 1 cut(s) 89
ScaI AGTACT 1 cut(s) 291
SchI GAGTC 2 cut(s) 214, 347
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 2 cut(s) 58, 364
SetI ASST 9 cut(s) 17, 42, 85, 139, 198, 281, 371, 387, 412
SpeI ACTAGT 1 cut(s) 176
Sse9I AATT 4 cut(s) 111, 143, 329, 416
SsiI CCGC 1 cut(s) 244
SspMI CTAG 1 cut(s) 177
StyD4I CCNGG 1 cut(s) 278
TaiI ACGT 1 cut(s) 42
TasI AATT 4 cut(s) 111, 143, 329, 416
TatI WGTACW 1 cut(s) 289
TauI GCSGC 1 cut(s) 246
TfiI GAWTC 2 cut(s) 260, 375
Tru1I TTAA 2 cut(s) 107, 332
Tru9I TTAA 2 cut(s) 107, 332
TseFI GTSAC 2 cut(s) 10, 217
TseI GCWGC 1 cut(s) 240
Tsp45I GTSAC 2 cut(s) 10, 217
TspDTI ATGAA 2 cut(s) 252, 262
TspGWI ACGGA 1 cut(s) 152
XapI RAATTY 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 1 cut(s) 177
ZrmI AGTACT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.