Prupe.7G192100_v2.0.a1

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0008378
Physical Location & Seq
Reverse (-)
18335148 .. 18337157
2010 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G192100.1

Sequence Viewer

Length: 699 bp
ATGGGGAGAGAGGTTCCACAACAACAGCAGCAGCAGGTGGCGTTGGCGGTGGAGCAGCTGCTGGCTGTGAATCCGTACAACCCCGACATGCTTTCCGATCTTGAAAACTACGTTAACGAGCAGGTTTCTTCACAAACTTATAATCTCGATGCCAATCTCTGCCTTCTTCGTATCTATCAGTTTGAGCCGGACCGTATGAGCACTCAAATTGTTGCCCGCATCTTGGTTAAGGCACTGATGGCAATGCCCTCCCCTGATTTCGGCCTTTGTCTATTCTTAATCCCAGAAAGAGTGCAAATGGAGGAGCAGTTCAAGACACTCACTGTCCTATCTCACTATTTGGAGACTGGAAGGTTCAGTCAATTCTGGGATGAAGCATCCAAGAATCGTCACATAGTTGAAGCTGTCCCAGGTTTTGAGCAAGCAATTCAAGCCTATGCAATTCATGTTCTTTCCTTGACGTACCAAAGGGTTCCCCGATCTGTGCTTGCTGAGGCTATCAATATTGAAGGCCTATCCCTGGACAAATTCCTTGAGCACCATGTAGCCAATAACGGTTGGATTCTTGAGAAAGGTCATGGAAGGGGTCAACTCATTGTCCTGCCTCGAAATGAATTTAACCATCCAGAGCTGAAAAAGAGCACAGACGATGGCATTCCATTAGAGCACGTGGCTCGTATCTTCCCCATTCTAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

233

Amino Acids

26.46

Weight (kDa)

5.35

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 141
AarI CACCTGC 1 cut(s) 25
Acc36I ACCTGC 2 cut(s) 25, 112
AciI CCGC 2 cut(s) 47, 217
AcsI RAATTY 2 cut(s) 527, 614
AcvI CACGTG 1 cut(s) 670
AfaI GTAC 2 cut(s) 77, 464
AfiI CCNNNNNNNGG 4 cut(s) 223, 260, 520, 692
AgsI TTSAA 5 cut(s) 104, 313, 401, 431, 509
AjnI CCWGG 2 cut(s) 409, 519
AloI GAACNNNNNNTCC 2 cut(s) 293, 325
AluBI AGCT 3 cut(s) 58, 404, 631
AluI AGCT 3 cut(s) 58, 404, 631
Alw21I GWGCWC 4 cut(s) 203, 540, 644, 669
Alw26I GTCTC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 61
AoxI GGCC 2 cut(s) 262, 511
ApeKI GCWGC 4 cut(s) 28, 31, 55, 58
ApoI RAATTY 2 cut(s) 527, 614
AspS9I GGNCC 1 cut(s) 190
AvaII GGWCC 1 cut(s) 190
BbrPI CACGTG 1 cut(s) 670
Bbv12I GWGCWC 4 cut(s) 203, 540, 644, 669
BbvCI CCTCAGC 1 cut(s) 492
BbvI GCAGC 4 cut(s) 40, 43, 45, 67
BccI CCATC 3 cut(s) 232, 630, 644
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 2 cut(s) 411, 521
BcoDI GTCTC 1 cut(s) 338
BfaI CTAG 1 cut(s) 692
BfuAI ACCTGC 2 cut(s) 25, 112
BisI GCNGC 4 cut(s) 29, 32, 56, 59
BlsI GCNGC 4 cut(s) 30, 33, 57, 60
Bme1390I CCNGG 2 cut(s) 411, 521
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 2 cut(s) 15, 474
BmrFI CCNGG 2 cut(s) 411, 521
BmsI GCATC 3 cut(s) 139, 228, 386
Bpu10I CCTNAGC 1 cut(s) 492
BpuEI CTTGAG 2 cut(s) 554, 587
BsaAI YACGTR 1 cut(s) 670
BsaBI GATNNNNATC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 409, 519
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 260, 520, 692
Bse1I ACTGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 153
BseBI CCWGG 2 cut(s) 411, 521
BseDI CCNNGG 2 cut(s) 409, 519
BseGI GGATG 3 cut(s) 376, 377, 622
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 4 cut(s) 223, 260, 520, 692
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 1 cut(s) 483
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 317
BseXI GCAGC 4 cut(s) 40, 43, 45, 67
BshFI GGCC 2 cut(s) 264, 513
BsiHKAI GWGCWC 4 cut(s) 203, 540, 644, 669
BsiSI CCGG 1 cut(s) 188
BslFI GGGAC 1 cut(s) 392
BslI CCNNNNNNNGG 4 cut(s) 223, 260, 520, 692
BsmAI GTCTC 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 392
BsmI GAATGC 1 cut(s) 654
BsnI GGCC 2 cut(s) 264, 513
Bsp1286I GDGCHC 4 cut(s) 203, 540, 644, 669
Bsp143I GATC 2 cut(s) 97, 479
BspACI CCGC 2 cut(s) 47, 217
BspANI GGCC 2 cut(s) 264, 513
BspCNI CTCAG 1 cut(s) 484
BspLI GGNNCC 2 cut(s) 15, 474
BspMI ACCTGC 2 cut(s) 25, 112
BsrDI GCAATG 1 cut(s) 249
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 409, 519
BssMI GATC 2 cut(s) 97, 479
Bst2UI CCWGG 2 cut(s) 411, 521
Bst4CI ACNGT 3 cut(s) 194, 325, 557
BstBAI YACGTR 1 cut(s) 670
BstC8I GCNNGC 4 cut(s) 63, 217, 423, 489
BstDEI CTNAG 1 cut(s) 492
BstF5I GGATG 3 cut(s) 376, 377, 622
BstKTI GATC 2 cut(s) 100, 482
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 2 cut(s) 97, 479
BstMWI GCNNNNNNNGC 2 cut(s) 239, 431
BstNI CCWGG 2 cut(s) 411, 521
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 2 cut(s) 409, 519
BstV1I GCAGC 4 cut(s) 40, 43, 45, 67
BsuRI GGCC 2 cut(s) 264, 513
BtsCI GGATG 3 cut(s) 376, 377, 622
BtsIMutI CAGTG 2 cut(s) 233, 321
BveI ACCTGC 2 cut(s) 25, 112
Cac8I GCNNGC 4 cut(s) 63, 217, 423, 489
CaiI CAGNNNCTG 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 190
CpoI CGGWCCG 1 cut(s) 190
Csp6I GTAC 2 cut(s) 76, 463
CspI CGGWCCG 1 cut(s) 190
CviAII CATG 4 cut(s) 88, 446, 542, 578
CviQI GTAC 2 cut(s) 76, 463
DdeI CTNAG 1 cut(s) 492
DpnI GATC 2 cut(s) 99, 481
DpnII GATC 2 cut(s) 97, 479
Eco147I AGGCCT 1 cut(s) 513
Eco47I GGWCC 1 cut(s) 190
Eco72I CACGTG 1 cut(s) 670
EcoRII CCWGG 2 cut(s) 409, 519
FaeI CATG 4 cut(s) 91, 449, 545, 581
FaiI YATR 8 cut(s) 89, 141, 197, 395, 438, 447, 543, 579
FaqI GGGAC 1 cut(s) 392
FatI CATG 4 cut(s) 87, 445, 541, 577
FauI CCCGC 1 cut(s) 224
Fnu4HI GCNGC 4 cut(s) 29, 32, 56, 59
FokI GGATG 3 cut(s) 364, 383, 609
Fsp4HI GCNGC 4 cut(s) 29, 32, 56, 59
FspBI CTAG 1 cut(s) 692
GluI GCNGC 4 cut(s) 29, 32, 56, 59
HaeIII GGCC 2 cut(s) 264, 513
HapII CCGG 1 cut(s) 188
Hin1II CATG 4 cut(s) 91, 449, 545, 581
HincII GTYRAC 2 cut(s) 115, 590
HindII GTYRAC 2 cut(s) 115, 590
HinfI GANTC 3 cut(s) 70, 385, 562
HpaI GTTAAC 1 cut(s) 115
HpaII CCGG 1 cut(s) 188
Hpy166II GTNNAC 2 cut(s) 115, 590
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 5 cut(s) 101, 146, 313, 566, 626
Hpy8I GTNNAC 2 cut(s) 115, 590
HpyAV CCTTC 4 cut(s) 173, 345, 503, 576
HpyCH4III ACNGT 3 cut(s) 194, 325, 557
HpyCH4IV ACGT 3 cut(s) 111, 461, 669
HpyCH4V TGCA 2 cut(s) 295, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 431
HpyF3I CTNAG 1 cut(s) 492
HpySE526I ACGT 3 cut(s) 111, 461, 669
Hsp92II CATG 4 cut(s) 91, 449, 545, 581
KspAI GTTAAC 1 cut(s) 115
Kzo9I GATC 2 cut(s) 97, 479
LmnI GCTCC 2 cut(s) 52, 304
Lsp1109I GCAGC 4 cut(s) 40, 43, 45, 67
LweI GCATC 3 cut(s) 139, 228, 386
MaeI CTAG 1 cut(s) 692
MaeII ACGT 3 cut(s) 111, 461, 669
MaeIII GTNAC 1 cut(s) 389
MalI GATC 2 cut(s) 99, 481
MboI GATC 2 cut(s) 97, 479
MboII GAAGA 3 cut(s) 120, 158, 673
MhlI GDGCHC 4 cut(s) 203, 540, 644, 669
MluCI AATT 6 cut(s) 207, 362, 426, 441, 527, 614
MmeI TCCRAC 1 cut(s) 539
MnlI CCTC 5 cut(s) 4, 259, 295, 487, 615
MseI TTAA 4 cut(s) 114, 228, 278, 618
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 1 cut(s) 188
MspR9I CCNGG 2 cut(s) 411, 521
Mva1269I GAATGC 1 cut(s) 654
MvaI CCWGG 2 cut(s) 411, 521
MwoI GCNNNNNNNGC 2 cut(s) 239, 431
NdeII GATC 2 cut(s) 97, 479
NlaIII CATG 4 cut(s) 91, 449, 545, 581
NlaIV GGNNCC 2 cut(s) 15, 474
NmuCI GTSAC 1 cut(s) 389
NspI RCATGY 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 25
PceI AGGCCT 1 cut(s) 513
PctI GAATGC 1 cut(s) 654
PfeI GAWTC 3 cut(s) 70, 385, 562
PkrI GCNGC 4 cut(s) 30, 33, 57, 60
PmaCI CACGTG 1 cut(s) 670
PmlI CACGTG 1 cut(s) 670
Ppu21I YACGTR 1 cut(s) 670
PsiI TTATAA 1 cut(s) 141
Psp6I CCWGG 2 cut(s) 409, 519
PspCI CACGTG 1 cut(s) 670
PspGI CCWGG 2 cut(s) 409, 519
PspN4I GGNNCC 2 cut(s) 15, 474
PspPI GGNCC 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 58
RsaI GTAC 2 cut(s) 77, 464
RsaNI GTAC 2 cut(s) 76, 463
Rsr2I CGGWCCG 1 cut(s) 190
RsrII CGGWCCG 1 cut(s) 190
SaqAI TTAA 4 cut(s) 114, 228, 278, 618
SatI GCNGC 4 cut(s) 29, 32, 56, 59
Sau3AI GATC 2 cut(s) 97, 479
Sau96I GGNCC 1 cut(s) 190
ScrFI CCNGG 2 cut(s) 411, 521
SduI GDGCHC 4 cut(s) 203, 540, 644, 669
SfaNI GCATC 3 cut(s) 139, 228, 386
SinI GGWCC 1 cut(s) 190
SmlI CTYRAG 2 cut(s) 533, 566
SmoI CTYRAG 2 cut(s) 533, 566
Sse9I AATT 6 cut(s) 207, 362, 426, 441, 527, 614
SseBI AGGCCT 1 cut(s) 513
SsiI CCGC 2 cut(s) 47, 217
SspI AATATT 1 cut(s) 505
SspMI CTAG 1 cut(s) 692
StuI AGGCCT 1 cut(s) 513
StyD4I CCNGG 2 cut(s) 409, 519
TaaI ACNGT 3 cut(s) 194, 325, 557
TaiI ACGT 3 cut(s) 114, 464, 672
TaqI TCGA 2 cut(s) 147, 607
TasI AATT 6 cut(s) 207, 362, 426, 441, 527, 614
TfiI GAWTC 3 cut(s) 70, 385, 562
Tru1I TTAA 4 cut(s) 114, 228, 278, 618
Tru9I TTAA 4 cut(s) 114, 228, 278, 618
TscAI CASTG 2 cut(s) 240, 328
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 4 cut(s) 28, 31, 55, 58
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 3 cut(s) 387, 434, 627
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 2 cut(s) 240, 328
VpaK11BI GGWCC 1 cut(s) 190
XapI RAATTY 2 cut(s) 527, 614
XceI RCATGY 1 cut(s) 91
XspI CTAG 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.