AT4G38300

glycosyl hydrolase family 10 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004763
Physical Location & Seq
Reverse (-)
17944556 .. 17945491
936 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G38300.1

Sequence Viewer

Length: 834 bp
ATGTGGTTCCCTCTTAACACTCATTTTCAAGCGAGAAAACGAGCAGTGACAATTCACGTGTCAAAAGAAAACGGAGAGAGCGTTGAAGGAGCAGAGGTGACAGTAGAGCAGATCTCTAAAGACTTCCCCATTGGATCTGCCATCTCCAAAACTATCCTTGGAAACATTCCTTACCAAGAATGGTTCGTCAAGAGATTCGACGCCACGGTATTCGAGAACGAGCTGAAATGGTACGCGACGGAGTCCGATCAAGGCAAACTCAACTACACATTGGCTGATAAGATGATGAATCTCGTCAGAGCTAACAGAATCATCGCTCGCGGCCACAACATATTCTGGGAGGATCCCAAATACAATCCCGACTGGGTTCGTAATCTAACCGGCGAAGATCTCCGGTCGGCGGTTAACCGGCGAATCAAGAGTCTAATGACTCGGTACAGAGGAGAGTTCGTGCATTGGGACGTGAGCAACGAGATGCTTCACTTCGACTTCTACGAGAGCAGACTGGGGAAGAACGTCATTGACTCGTTGGCGACTCTGTTCTTCAATGATTTCAACGTGGTGGAGACTTGCAGCGACGAGAAGTCAACGGTTGACGAATACATCGCCAGGGTGAGGGAACTCCAACGGTACGACGGCATAAGGATGGACGGAATCGGTCTCGAGGGTCACTTCACGACGCCAAACGTAGCGCTGATGAGAGCCATCCTCGATAAACTCGCTACGCTCCAGCTCCCAATCTGGCTCACCGAGATTGATATCAGCAGCAGCCTCGACCACCGCTCTCAGGTTCACAAGTCTCTGTTTTCCGGTTTAGTTCAGTTTGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_10

Protein Analysis

277

Amino Acids

32.1

Weight (kDa)

5.93

Isoelectric Point (pI)

31.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 37 - 261 1.8e-36 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 783
AccII CGCG 2 cut(s) 236, 321
AciI CCGC 3 cut(s) 321, 401, 781
AclWI GGATC 3 cut(s) 142, 338, 351
AcoI YGGCCR 1 cut(s) 322
AcvI CACGTG 1 cut(s) 58
AcyI GRCGYC 2 cut(s) 201, 680
AfaI GTAC 3 cut(s) 233, 437, 632
AfeI AGCGCT 1 cut(s) 693
AfiI CCNNNNNNNGG 3 cut(s) 400, 615, 787
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 4 cut(s) 29, 86, 547, 556
AhdI GACNNNNNGTC 1 cut(s) 583
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 1 cut(s) 608
AluBI AGCT 3 cut(s) 223, 302, 733
AluI AGCT 3 cut(s) 223, 302, 733
Alw26I GTCTC 3 cut(s) 560, 665, 804
AlwI GGATC 3 cut(s) 142, 338, 351
Ama87I CYCGRG 1 cut(s) 662
Aor51HI AGCGCT 1 cut(s) 693
AoxI GGCC 1 cut(s) 322
ApeKI GCWGC 3 cut(s) 573, 765, 768
ArsI GACNNNNNNTTYG 2 cut(s) 406, 438
AspLEI GCGC 1 cut(s) 694
AsuHPI GGTGA 3 cut(s) 109, 625, 739
AvaI CYCGRG 1 cut(s) 662
BamHI GGATCC 1 cut(s) 343
BbrPI CACGTG 1 cut(s) 58
BbvI GCAGC 3 cut(s) 585, 777, 780
BccI CCATC 3 cut(s) 149, 640, 713
BceAI ACGGC 1 cut(s) 652
BcgI CGANNNNNNTGC 2 cut(s) 754, 788
BciT130I CCWGG 1 cut(s) 610
BcoDI GTCTC 3 cut(s) 560, 665, 804
BfoI RGCGCY 1 cut(s) 695
BglII AGATCT 2 cut(s) 111, 388
BisI GCNGC 4 cut(s) 322, 574, 766, 769
BlsI GCNGC 4 cut(s) 323, 575, 767, 770
Bme1390I CCNGG 1 cut(s) 610
BmeRI GACNNNNNGTC 1 cut(s) 583
BmeT110I CYCGRG 1 cut(s) 662
BmgBI CACGTC 1 cut(s) 463
BmiI GGNNCC 2 cut(s) 8, 345
BmrFI CCNGG 1 cut(s) 610
BmrI ACTGGG 2 cut(s) 373, 515
BmsI GCATC 1 cut(s) 465
BmuI ACTGGG 2 cut(s) 373, 515
BplI GAGNNNNNCTC 4 cut(s) 98, 130, 693, 725
BpmI CTGGAG 1 cut(s) 713
BsaAI YACGTR 1 cut(s) 58
BsaBI GATNNNNATC 1 cut(s) 758
BsaHI GRCGYC 2 cut(s) 201, 680
BsaI GGTCTC 1 cut(s) 665
BsaJI CCNNGG 3 cut(s) 157, 204, 609
BsaWI WCCGGW 2 cut(s) 393, 809
BsaXI ACNNNNNCTCC 6 cut(s) 66, 81, 96, 111, 435, 465
Bsc4I CCNNNNNNNGG 3 cut(s) 400, 615, 787
Bse118I RCCGGY 2 cut(s) 380, 408
Bse1I ACTGG 2 cut(s) 368, 510
Bse8I GATNNNNATC 1 cut(s) 758
BseBI CCWGG 1 cut(s) 610
BseDI CCNNGG 3 cut(s) 157, 204, 609
BseGI GGATG 2 cut(s) 651, 705
BseJI GATNNNNATC 1 cut(s) 758
BseLI CCNNNNNNNGG 3 cut(s) 400, 615, 787
BseMII CTCAG 1 cut(s) 800
BseNI ACTGG 2 cut(s) 368, 510
BseRI GAGGAG 1 cut(s) 456
BseXI GCAGC 3 cut(s) 585, 777, 780
Bsh1236I CGCG 2 cut(s) 236, 321
Bsh1285I CGRYCG 1 cut(s) 398
BshFI GGCC 1 cut(s) 324
BsiEI CGRYCG 1 cut(s) 398
BsiHKCI CYCGRG 1 cut(s) 662
BsiSI CCGG 4 cut(s) 381, 394, 409, 810
BslFI GGGAC 1 cut(s) 473
BslI CCNNNNNNNGG 3 cut(s) 400, 615, 787
BsmAI GTCTC 3 cut(s) 560, 665, 804
BsmFI GGGAC 1 cut(s) 473
BsnI GGCC 1 cut(s) 324
Bso31I GGTCTC 1 cut(s) 665
BsoBI CYCGRG 1 cut(s) 662
Bsp143I GATC 5 cut(s) 111, 134, 247, 343, 388
BspACI CCGC 3 cut(s) 321, 401, 781
BspANI GGCC 1 cut(s) 324
BspCNI CTCAG 1 cut(s) 799
BspFNI CGCG 2 cut(s) 236, 321
BspLI GGNNCC 2 cut(s) 8, 345
BspPI GGATC 3 cut(s) 142, 338, 351
BspTNI GGTCTC 1 cut(s) 665
BsrBI CCGCTC 1 cut(s) 783
BsrFI RCCGGY 2 cut(s) 380, 408
BsrI ACTGG 2 cut(s) 368, 510
BssAI RCCGGY 2 cut(s) 380, 408
BssECI CCNNGG 3 cut(s) 157, 204, 609
BssMI GATC 5 cut(s) 111, 134, 247, 343, 388
BssNI GRCGYC 2 cut(s) 201, 680
BssT1I CCWWGG 1 cut(s) 157
Bst2UI CCWGG 1 cut(s) 610
Bst4CI ACNGT 4 cut(s) 103, 208, 592, 630
BstACI GRCGYC 2 cut(s) 201, 680
BstBAI YACGTR 1 cut(s) 58
BstC8I GCNNGC 1 cut(s) 319
BstDEI CTNAG 1 cut(s) 786
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 2 cut(s) 651, 705
BstFNI CGCG 2 cut(s) 236, 321
BstH2I RGCGCY 1 cut(s) 695
BstHHI GCGC 1 cut(s) 694
BstKTI GATC 5 cut(s) 114, 137, 250, 346, 391
BstMAI GTCTC 3 cut(s) 560, 665, 804
BstMBI GATC 5 cut(s) 111, 134, 247, 343, 388
BstMCI CGRYCG 1 cut(s) 398
BstNI CCWGG 1 cut(s) 610
BstSCI CCNGG 1 cut(s) 608
BstUI CGCG 2 cut(s) 236, 321
BstV1I GCAGC 3 cut(s) 585, 777, 780
BstX2I RGATCY 4 cut(s) 111, 134, 343, 388
BstYI RGATCY 4 cut(s) 111, 134, 343, 388
BsuRI GGCC 1 cut(s) 324
BtgI CCRYGG 1 cut(s) 204
BtgZI GCGATG 2 cut(s) 298, 589
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 2 cut(s) 651, 705
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 1 cut(s) 51
Cac8I GCNNGC 1 cut(s) 319
CfoI GCGC 1 cut(s) 694
Cfr10I RCCGGY 2 cut(s) 380, 408
CseI GACGC 2 cut(s) 209, 688
Csp6I GTAC 3 cut(s) 232, 436, 631
CviJI RGCY 8 cut(s) 223, 275, 302, 324, 704, 733, 745, 771
CviKI_1 RGCY 8 cut(s) 223, 275, 302, 324, 704, 733, 745, 771
CviQI GTAC 3 cut(s) 232, 436, 631
DdeI CTNAG 1 cut(s) 786
DpnI GATC 5 cut(s) 113, 136, 249, 345, 390
DpnII GATC 5 cut(s) 111, 134, 247, 343, 388
DriI GACNNNNNGTC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 322
Eam1105I GACNNNNNGTC 1 cut(s) 583
Eco130I CCWWGG 1 cut(s) 157
Eco31I GGTCTC 1 cut(s) 665
Eco32I GATATC 1 cut(s) 760
Eco47III AGCGCT 1 cut(s) 693
Eco72I CACGTG 1 cut(s) 58
Eco88I CYCGRG 1 cut(s) 662
EcoRII CCWGG 1 cut(s) 608
EcoRV GATATC 1 cut(s) 760
EcoT14I CCWWGG 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 157
FaiI YATR 2 cut(s) 332, 641
FaqI GGGAC 1 cut(s) 473
Fnu4HI GCNGC 4 cut(s) 322, 574, 766, 769
FokI GGATG 2 cut(s) 658, 692
Fsp4HI GCNGC 4 cut(s) 322, 574, 766, 769
GlaI GCGC 1 cut(s) 693
GluI GCNGC 4 cut(s) 322, 574, 766, 769
GsuI CTGGAG 1 cut(s) 713
HaeII RGCGCY 1 cut(s) 695
HaeIII GGCC 1 cut(s) 324
HapII CCGG 4 cut(s) 381, 394, 409, 810
HgaI GACGC 2 cut(s) 209, 688
HhaI GCGC 1 cut(s) 694
Hin1I GRCGYC 2 cut(s) 201, 680
Hin6I GCGC 1 cut(s) 692
HinP1I GCGC 1 cut(s) 692
HincII GTYRAC 3 cut(s) 406, 588, 595
HindII GTYRAC 3 cut(s) 406, 588, 595
HpaI GTTAAC 1 cut(s) 406
HpaII CCGG 4 cut(s) 381, 394, 409, 810
HphI GGTGA 3 cut(s) 109, 625, 739
Hpy166II GTNNAC 4 cut(s) 406, 588, 595, 793
Hpy188I TCNGA 2 cut(s) 247, 299
Hpy188III TCNNGA 6 cut(s) 190, 214, 359, 418, 662, 676
Hpy8I GTNNAC 4 cut(s) 406, 588, 595, 793
Hpy99I CGWCG 5 cut(s) 203, 241, 581, 638, 682
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 4 cut(s) 103, 208, 592, 630
HpyCH4IV ACGT 5 cut(s) 57, 462, 516, 558, 687
HpyCH4V TGCA 3 cut(s) 454, 573, 827
HpyF3I CTNAG 1 cut(s) 786
HpySE526I ACGT 5 cut(s) 57, 462, 516, 558, 687
Hsp92I GRCGYC 2 cut(s) 201, 680
HspAI GCGC 1 cut(s) 692
KspAI GTTAAC 1 cut(s) 406
Kzo9I GATC 5 cut(s) 111, 134, 247, 343, 388
LmnI GCTCC 3 cut(s) 89, 732, 738
Lsp1109I GCAGC 3 cut(s) 585, 777, 780
LweI GCATC 1 cut(s) 465
MaeII ACGT 5 cut(s) 57, 462, 516, 558, 687
MaeIII GTNAC 3 cut(s) 46, 97, 668
MalI GATC 5 cut(s) 113, 136, 249, 345, 390
MbiI CCGCTC 1 cut(s) 783
MboI GATC 5 cut(s) 111, 134, 247, 343, 388
MboII GAAGA 3 cut(s) 398, 523, 535
MflI RGATCY 4 cut(s) 111, 134, 343, 388
MluCI AATT 2 cut(s) 51, 828
MlyI GAGTC 5 cut(s) 251, 424, 430, 518, 529
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 8 cut(s) 21, 88, 334, 434, 609, 658, 719, 782
MseI TTAA 2 cut(s) 15, 405
MslI CAYNNNNRTG 1 cut(s) 644
MspI CCGG 4 cut(s) 381, 394, 409, 810
MspR9I CCNGG 1 cut(s) 610
MvaI CCWGG 1 cut(s) 610
MvnI CGCG 2 cut(s) 236, 321
NdeII GATC 5 cut(s) 111, 134, 247, 343, 388
NlaIV GGNNCC 2 cut(s) 8, 345
NmuCI GTSAC 3 cut(s) 46, 97, 668
PaeR7I CTCGAG 1 cut(s) 662
PcsI WCGNNNNNNNCGW 3 cut(s) 78, 468, 492
PfeI GAWTC 5 cut(s) 195, 289, 309, 414, 654
PflFI GACNNNGTC 1 cut(s) 241
PkrI GCNGC 4 cut(s) 323, 575, 767, 770
PleI GAGTC 5 cut(s) 250, 424, 429, 518, 529
PmaCI CACGTG 1 cut(s) 58
PmlI CACGTG 1 cut(s) 58
PpsI GAGTC 5 cut(s) 250, 424, 429, 518, 529
Ppu21I YACGTR 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 608
PspCI CACGTG 1 cut(s) 58
PspGI CCWGG 1 cut(s) 608
PspN4I GGNNCC 2 cut(s) 8, 345
PsuI RGATCY 4 cut(s) 111, 134, 343, 388
PsyI GACNNNGTC 1 cut(s) 241
RsaI GTAC 3 cut(s) 233, 437, 632
RsaNI GTAC 3 cut(s) 232, 436, 631
RseI CAYNNNNRTG 1 cut(s) 644
SaqAI TTAA 2 cut(s) 15, 405
SatI GCNGC 4 cut(s) 322, 574, 766, 769
Sau3AI GATC 5 cut(s) 111, 134, 247, 343, 388
SchI GAGTC 5 cut(s) 251, 424, 430, 518, 529
ScrFI CCNGG 1 cut(s) 610
SfaNI GCATC 1 cut(s) 465
Sfr274I CTCGAG 1 cut(s) 662
SlaI CTCGAG 1 cut(s) 662
SmiMI CAYNNNNRTG 1 cut(s) 644
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
Sse9I AATT 2 cut(s) 51, 828
SsiI CCGC 3 cut(s) 321, 401, 781
StyD4I CCNGG 1 cut(s) 608
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 4 cut(s) 103, 208, 592, 630
TaiI ACGT 5 cut(s) 60, 465, 519, 561, 690
TaqI TCGA 6 cut(s) 198, 213, 486, 663, 711, 774
TaqII GACCGA 1 cut(s) 647
TasI AATT 2 cut(s) 51, 828
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 5 cut(s) 195, 289, 309, 414, 654
Tru1I TTAA 2 cut(s) 15, 405
Tru9I TTAA 2 cut(s) 15, 405
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 3 cut(s) 46, 97, 668
TseI GCWGC 3 cut(s) 573, 765, 768
Tsp45I GTSAC 3 cut(s) 46, 97, 668
TspDTI ATGAA 1 cut(s) 302
TspGWI ACGGA 3 cut(s) 87, 254, 666
TspRI CASTG 1 cut(s) 51
Tth111I GACNNNGTC 1 cut(s) 241
XhoI CTCGAG 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.