FvH4_2g01510

Endo-1,4-beta-xylanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004763
Physical Location & Seq
Forward (+)
1386995 .. 1390155
3161 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01510.t2

Sequence Viewer

Length: 1671 bp
ATGGGAAGCACAATTCTCATCTTTGTTAGGCTGCAACTTCTCATGTTCTTTCTCCTCCTGGGCTCACCCTTTGTTTTCTCCTTTGATGGTCCACTATACGACTCTACTGCTTACACTGAGTGTAAAAGGCAACCGGAGAAACCTCTATATGGTGGAGGGATCATTGTTGATGAGCCTTCAGCTGTTGTGGACAGAGACAAGGGAGGCGTTTATTCACCGGCATTTCTACTCAAAAATCTCACCGGAAATGGCACCATTTACAGCTTCTCTAGTTGGGTGAAGCTAAGGAGTGCGGGTTCAGCTATGATACAGGCAAGTCTAAAGGCAGAAGATGAAACATACAGTTGTATAGGAACTGTTTTGGCCAAGCGAGGATGCTGGTCATTCCTCAAGGGTGGCTTTGTCCTAGATTCACCCTCTAATCTCTCTATACTATTCTTCCAGAATACAGATGGCAGAGATGTCAGCCTAGCAATTGCAAGTGCTTCTTTGCAGCCATTTAGTGTTCAGCAATGGAACACTTATCAGCAGTATATGATCAACACTAAAAGGAAGCGTGCCGTGACGGTCCATGTCTCAAATGAACAAGGAAAGGGGCTGCAAGGAGCTGCCATTACCGTAGAGCAAGTCTCCAAAGATTTCCCATTTGGATCTGCAATTTCAAAGACCATACTAGGAAATTTGCCCTACCAGAATTGGTTTGTGGAGAGATTCAATGCTGCGGTGTTTGAGAACGAACTCAAGTGGTATGCAACAGAACCTCAACAAGGCAATGTCACTTACATTATAGCAGATCAGATGTTACAATTTGTTCGGGCTAACAAAATCACTACTAGAGGGCACAATATATTCTGGGAAGATCCCAAATACACACCAGCTTGGGTTCGAAACCTCACAAGTACTGATCAACTAAAATCAGCTGTCAACTCTCGGATACAAAGCCTAATGACCAAATACAAAGAAGAGTTCATTCATTGGGATGTCAGCAATGAAATGCTTCACTTTGATTTCTATGAGAAACAACTTGGCCCCAATGCCACCTTGGATTTCTTTCAGACGGCACACAAATCCGATCCTTTGGCAACCCTTTTTATGAACGAATTTAATGTGGTGGAGACCTGCACTGATGTGAATTCCACAGTTGATACCTATGTTTCAAGGCTGAGGGAACTTCAACAAGGTGGAGTATCAATGGATGGGATTGGACTAGAGGGTCATTTCACAAGACCAAACCCCCCTCTAATGAGAGCTATTATAGACAAGTTGGCTACTCTAGGCCTTCCTATTTGGCTCACAGAGGTTGACATCAGCAATACTCTAGACAAAGAAACACAGGCCATTTATCTAGAGCAAGTTTTAAGAGAAGGTTTCTCTCATCCATCTGTAAATGGGATAATGCTCTGGACTGCTCTACACCCCTACGGGTGCTATGAGATGTGCCTGACGGATAATAATCTTCAAAATCTACCAGCCGGTGACGTGGTGGACAGGCTCTTGAAAGAATGGCAAACCAGGAAAATAGAAGCTCAGACCGATGAGCATGGATCACATAGCTTTTATGGGTTTTTGGGTGAGTACAGGGTTAGTGTCAAGTACAGTAATAGGACTACAAACTCAACATTCTCACTGTGCCAAGGTGAAGAAACTAGACATGTTAGCATTCTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_10

Protein Analysis

557

Amino Acids

62.72

Weight (kDa)

5.74

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 186 - 449 3e-48 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1212
Acc36I ACCTGC 1 cut(s) 1127
AccB1I GGYRCC 1 cut(s) 251
AciI CCGC 2 cut(s) 293, 722
AclWI GGATC 5 cut(s) 167, 658, 854, 1067, 1552
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 3 cut(s) 679, 1100, 1132
AcuI CTGAAG 1 cut(s) 162
AdeI CACNNNGTG 1 cut(s) 120
AfaI GTAC 3 cut(s) 901, 1577, 1595
AfiI CCNNNNNNNGG 2 cut(s) 149, 767
AflIII ACRYGT 1 cut(s) 1651
AgsI TTSAA 6 cut(s) 663, 715, 1158, 1175, 1460, 1498
AjiI CACGTC 1 cut(s) 1480
AjnI CCWGG 2 cut(s) 57, 1511
AleI CACNNNNGTG 1 cut(s) 1127
AloI GAACNNNNNNTCC 2 cut(s) 280, 312
Alw26I GTCTC 4 cut(s) 189, 580, 634, 1109
AlwI GGATC 5 cut(s) 167, 658, 854, 1067, 1552
AoxI GGCC 4 cut(s) 363, 1027, 1276, 1335
ApeKI GCWGC 5 cut(s) 31, 493, 598, 608, 719
ApoI RAATTY 3 cut(s) 679, 1100, 1132
Asp700I GAANNNNTTC 1 cut(s) 996
AspS9I GGNCC 3 cut(s) 89, 568, 1028
AsuHPI GGTGA 8 cut(s) 57, 207, 232, 289, 405, 1487, 1583, 1649
AsuII TTCGAA 1 cut(s) 886
AvaII GGWCC 2 cut(s) 89, 568
BaeGI GKGCMC 1 cut(s) 843
BaeI ACNNNNGTAYC 2 cut(s) 1137, 1170
BalI TGGCCA 1 cut(s) 365
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 65
BbvCI CCTCAGC 1 cut(s) 1163
BbvI GCAGC 5 cut(s) 18, 505, 585, 595, 706
BccI CCATC 4 cut(s) 80, 446, 1190, 1387
BceAI ACGGC 2 cut(s) 545, 1074
BcgI CGANNNNNNTGC 2 cut(s) 89, 123
BciT130I CCWGG 2 cut(s) 59, 1513
BciVI GTATCC 1 cut(s) 927
BclI TGATCA 2 cut(s) 537, 904
BcoDI GTCTC 4 cut(s) 189, 580, 634, 1109
BfuAI ACCTGC 1 cut(s) 1127
BfuI GTATCC 1 cut(s) 927
BisI GCNGC 5 cut(s) 32, 494, 599, 609, 720
BlsI GCNGC 5 cut(s) 33, 495, 600, 610, 721
BmcAI AGTACT 1 cut(s) 901
Bme1390I CCNGG 2 cut(s) 59, 1513
Bme18I GGWCC 2 cut(s) 89, 568
BmgBI CACGTC 1 cut(s) 1480
BmgT120I GGNCC 3 cut(s) 89, 568, 1028
BmiI GGNNCC 2 cut(s) 253, 1030
BmrFI CCNGG 2 cut(s) 59, 1513
BmsI GCATC 1 cut(s) 365
BplI GAGNNNNNCTC 2 cut(s) 614, 646
Bpu10I CCTNAGC 2 cut(s) 284, 1163
Bpu14I TTCGAA 1 cut(s) 886
BpuEI CTTGAG 2 cut(s) 374, 725
BsaBI GATNNNNATC 1 cut(s) 1452
BsaI GGTCTC 1 cut(s) 1109
BsaJI CCNNGG 3 cut(s) 58, 1041, 1633
BsaWI WCCGGW 2 cut(s) 133, 242
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 767
Bse118I RCCGGY 2 cut(s) 217, 1472
Bse3DI GCAATG 3 cut(s) 518, 778, 994
Bse8I GATNNNNATC 1 cut(s) 1452
BseBI CCWGG 2 cut(s) 59, 1513
BseDI CCNNGG 3 cut(s) 58, 1041, 1633
BseGI GGATG 4 cut(s) 380, 985, 1201, 1375
BseJI GATNNNNATC 1 cut(s) 1452
BseLI CCNNNNNNNGG 2 cut(s) 149, 767
BseMI GCAATG 3 cut(s) 518, 778, 994
BseMII CTCAG 3 cut(s) 108, 1154, 1541
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 843
BseXI GCAGC 5 cut(s) 18, 505, 585, 595, 706
BsgI GTGCAG 1 cut(s) 1105
BshFI GGCC 4 cut(s) 365, 1029, 1278, 1337
BshNI GGYRCC 1 cut(s) 251
BsiSI CCGG 4 cut(s) 134, 218, 243, 1473
BslI CCNNNNNNNGG 2 cut(s) 149, 767
BsmAI GTCTC 4 cut(s) 189, 580, 634, 1109
BsmI GAATGC 1 cut(s) 1659
BsnI GGCC 4 cut(s) 365, 1029, 1278, 1337
Bso31I GGTCTC 1 cut(s) 1109
Bsp119I TTCGAA 1 cut(s) 886
Bsp1286I GDGCHC 2 cut(s) 65, 843
Bsp143I GATC 8 cut(s) 159, 537, 650, 793, 859, 904, 1072, 1544
BspACI CCGC 2 cut(s) 293, 722
BspANI GGCC 4 cut(s) 365, 1029, 1278, 1337
BspCNI CTCAG 3 cut(s) 109, 1155, 1540
BspLI GGNNCC 2 cut(s) 253, 1030
BspMI ACCTGC 1 cut(s) 1127
BspPI GGATC 5 cut(s) 167, 658, 854, 1067, 1552
BspT104I TTCGAA 1 cut(s) 886
BspT107I GGYRCC 1 cut(s) 251
BspTNI GGTCTC 1 cut(s) 1109
BsrDI GCAATG 3 cut(s) 518, 778, 994
BsrFI RCCGGY 2 cut(s) 217, 1472
BssAI RCCGGY 2 cut(s) 217, 1472
BssECI CCNNGG 3 cut(s) 58, 1041, 1633
BssMI GATC 8 cut(s) 159, 537, 650, 793, 859, 904, 1072, 1544
BssT1I CCWWGG 2 cut(s) 1041, 1633
Bst2UI CCWGG 2 cut(s) 59, 1513
Bst4CI ACNGT 7 cut(s) 344, 358, 568, 619, 1141, 1598, 1629
Bst6I CTCTTC 1 cut(s) 957
BstBI TTCGAA 1 cut(s) 886
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 4 cut(s) 117, 284, 1163, 1527
BstENI CCTNNNNNAGG 1 cut(s) 765
BstF5I GGATG 4 cut(s) 380, 985, 1201, 1375
BstKTI GATC 8 cut(s) 162, 540, 653, 796, 862, 907, 1075, 1547
BstMAI GTCTC 4 cut(s) 189, 580, 634, 1109
BstMBI GATC 8 cut(s) 159, 537, 650, 793, 859, 904, 1072, 1544
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstNI CCWGG 2 cut(s) 59, 1513
BstNSI RCATGY 1 cut(s) 1655
BstSCI CCNGG 2 cut(s) 57, 1511
BstSLI GKGCMC 1 cut(s) 843
BstV1I GCAGC 5 cut(s) 18, 505, 585, 595, 706
BstX2I RGATCY 2 cut(s) 650, 859
BstYI RGATCY 2 cut(s) 650, 859
BsuI GTATCC 1 cut(s) 927
BsuRI GGCC 4 cut(s) 365, 1029, 1278, 1337
BtrI CACGTC 1 cut(s) 1480
BtsCI GGATG 4 cut(s) 380, 985, 1201, 1375
BtsIMutI CAGTG 3 cut(s) 114, 1122, 1625
BveI ACCTGC 1 cut(s) 1127
Cac8I GCNNGC 1 cut(s) 558
Cfr10I RCCGGY 2 cut(s) 217, 1472
Cfr13I GGNCC 3 cut(s) 89, 568, 1028
Csp6I GTAC 3 cut(s) 900, 1576, 1594
CviAII CATG 4 cut(s) 43, 572, 1541, 1652
CviQI GTAC 3 cut(s) 900, 1576, 1594
DdeI CTNAG 4 cut(s) 117, 284, 1163, 1527
DpnI GATC 8 cut(s) 161, 539, 652, 795, 861, 906, 1074, 1546
DpnII GATC 8 cut(s) 159, 537, 650, 793, 859, 904, 1072, 1544
DraIII CACNNNGTG 1 cut(s) 120
DrdI GACNNNNNNGTC 1 cut(s) 1212
DseDI GACNNNNNNGTC 1 cut(s) 1212
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 957
EarI CTCTTC 1 cut(s) 957
Eco130I CCWWGG 2 cut(s) 1041, 1633
Eco147I AGGCCT 1 cut(s) 1278
Eco24I GRGCYC 1 cut(s) 65
Eco31I GGTCTC 1 cut(s) 1109
Eco47I GGWCC 2 cut(s) 89, 568
Eco57I CTGAAG 1 cut(s) 162
EcoNI CCTNNNNNAGG 1 cut(s) 765
EcoRI GAATTC 1 cut(s) 1132
EcoRII CCWGG 2 cut(s) 57, 1511
EcoT14I CCWWGG 2 cut(s) 1041, 1633
EcoT38I GRGCYC 1 cut(s) 65
ErhI CCWWGG 2 cut(s) 1041, 1633
FaeI CATG 4 cut(s) 46, 575, 1544, 1655
FalI AAGNNNNNCTT 4 cut(s) 383, 415, 472, 504
FatI CATG 4 cut(s) 42, 571, 1540, 1651
FauI CCCGC 1 cut(s) 286
FbaI TGATCA 2 cut(s) 537, 904
Fnu4HI GCNGC 5 cut(s) 32, 494, 599, 609, 720
FokI GGATG 4 cut(s) 387, 992, 1208, 1362
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 5 cut(s) 32, 494, 599, 609, 720
GluI GCNGC 5 cut(s) 32, 494, 599, 609, 720
HaeIII GGCC 4 cut(s) 365, 1029, 1278, 1337
HapII CCGG 4 cut(s) 134, 218, 243, 1473
Hin1II CATG 4 cut(s) 46, 575, 1544, 1655
HincII GTYRAC 2 cut(s) 925, 1303
HindII GTYRAC 2 cut(s) 925, 1303
HinfI GANTC 3 cut(s) 101, 410, 711
HpaII CCGG 4 cut(s) 134, 218, 243, 1473
HphI GGTGA 8 cut(s) 57, 207, 232, 289, 405, 1487, 1583, 1649
Hpy166II GTNNAC 5 cut(s) 92, 190, 925, 1303, 1486
Hpy188I TCNGA 5 cut(s) 798, 933, 1056, 1072, 1530
Hpy188III TCNNGA 5 cut(s) 442, 1319, 1346, 1402, 1495
Hpy8I GTNNAC 5 cut(s) 92, 190, 925, 1303, 1486
HpyAV CCTTC 3 cut(s) 186, 1289, 1358
HpyCH4III ACNGT 7 cut(s) 344, 358, 568, 619, 1141, 1598, 1629
HpyCH4IV ACGT 1 cut(s) 1479
HpyCH4V TGCA 7 cut(s) 34, 479, 493, 601, 656, 752, 1122
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 4 cut(s) 117, 284, 1163, 1527
HpySE526I ACGT 1 cut(s) 1479
Hsp92II CATG 4 cut(s) 46, 575, 1544, 1655
Ksp22I TGATCA 2 cut(s) 537, 904
Kzo9I GATC 8 cut(s) 159, 537, 650, 793, 859, 904, 1072, 1544
LmnI GCTCC 1 cut(s) 605
Lsp1109I GCAGC 5 cut(s) 18, 505, 585, 595, 706
LweI GCATC 1 cut(s) 365
MaeII ACGT 1 cut(s) 1479
MaeIII GTNAC 4 cut(s) 562, 775, 801, 1475
MalI GATC 8 cut(s) 161, 539, 652, 795, 861, 906, 1074, 1546
MboI GATC 8 cut(s) 159, 537, 650, 793, 859, 904, 1072, 1544
MboII GAAGA 6 cut(s) 341, 430, 869, 974, 1448, 1652
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 2 cut(s) 650, 859
MhlI GDGCHC 2 cut(s) 65, 843
MlsI TGGCCA 1 cut(s) 365
MluCI AATT 8 cut(s) 12, 474, 657, 679, 694, 806, 1100, 1132
MluNI TGGCCA 1 cut(s) 365
MlyI GAGTC 1 cut(s) 95
Mox20I TGGCCA 1 cut(s) 365
MroXI GAANNNNTTC 1 cut(s) 996
MscI TGGCCA 1 cut(s) 365
MseI TTAA 2 cut(s) 1104, 1358
MslI CAYNNNNRTG 2 cut(s) 978, 1127
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 2 cut(s) 182, 920
MspI CCGG 4 cut(s) 134, 218, 243, 1473
MspR9I CCNGG 2 cut(s) 59, 1513
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 1 cut(s) 1659
MvaI CCWGG 2 cut(s) 59, 1513
MwoI GCNNNNNNNGC 1 cut(s) 299
NdeII GATC 8 cut(s) 159, 537, 650, 793, 859, 904, 1072, 1544
NlaIII CATG 4 cut(s) 46, 575, 1544, 1655
NlaIV GGNNCC 2 cut(s) 253, 1030
NmuCI GTSAC 3 cut(s) 562, 775, 1475
NspI RCATGY 1 cut(s) 1655
NspV TTCGAA 1 cut(s) 886
OliI CACNNNNGTG 1 cut(s) 1127
PceI AGGCCT 1 cut(s) 1278
PciI ACATGT 1 cut(s) 1651
PctI GAATGC 1 cut(s) 1659
PdmI GAANNNNTTC 1 cut(s) 996
PfeI GAWTC 2 cut(s) 410, 711
PkrI GCNGC 5 cut(s) 33, 495, 600, 610, 721
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PscI ACATGT 1 cut(s) 1651
Psp6I CCWGG 2 cut(s) 57, 1511
PspGI CCWGG 2 cut(s) 57, 1511
PspN4I GGNNCC 2 cut(s) 253, 1030
PspPI GGNCC 3 cut(s) 89, 568, 1028
PsuI RGATCY 2 cut(s) 650, 859
PvuII CAGCTG 2 cut(s) 182, 920
RsaI GTAC 3 cut(s) 901, 1577, 1595
RsaNI GTAC 3 cut(s) 900, 1576, 1594
RseI CAYNNNNRTG 2 cut(s) 978, 1127
SaqAI TTAA 2 cut(s) 1104, 1358
SatI GCNGC 5 cut(s) 32, 494, 599, 609, 720
Sau3AI GATC 8 cut(s) 159, 537, 650, 793, 859, 904, 1072, 1544
Sau96I GGNCC 3 cut(s) 89, 568, 1028
ScaI AGTACT 1 cut(s) 901
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 2 cut(s) 59, 1513
SduI GDGCHC 2 cut(s) 65, 843
SfaNI GCATC 1 cut(s) 365
SfuI TTCGAA 1 cut(s) 886
SinI GGWCC 2 cut(s) 89, 568
SmiMI CAYNNNNRTG 2 cut(s) 978, 1127
SmlI CTYRAG 2 cut(s) 389, 740
SmoI CTYRAG 2 cut(s) 389, 740
Sse9I AATT 8 cut(s) 12, 474, 657, 679, 694, 806, 1100, 1132
SseBI AGGCCT 1 cut(s) 1278
SsiI CCGC 2 cut(s) 293, 722
StuI AGGCCT 1 cut(s) 1278
StyD4I CCNGG 2 cut(s) 57, 1511
StyI CCWWGG 2 cut(s) 1041, 1633
TaaI ACNGT 7 cut(s) 344, 358, 568, 619, 1141, 1598, 1629
TaiI ACGT 1 cut(s) 1482
TaqI TCGA 1 cut(s) 886
TaqII GACCGA 1 cut(s) 1547
TasI AATT 8 cut(s) 12, 474, 657, 679, 694, 806, 1100, 1132
TatI WGTACW 3 cut(s) 899, 1575, 1593
TfiI GAWTC 2 cut(s) 410, 711
Tru1I TTAA 2 cut(s) 1104, 1358
Tru9I TTAA 2 cut(s) 1104, 1358
TscAI CASTG 3 cut(s) 121, 1129, 1632
TseFI GTSAC 3 cut(s) 562, 775, 1475
TseI GCWGC 5 cut(s) 31, 493, 598, 608, 719
Tsp45I GTSAC 3 cut(s) 562, 775, 1475
TspDTI ATGAA 6 cut(s) 348, 597, 958, 962, 1005, 1109
TspGWI ACGGA 1 cut(s) 1460
TspRI CASTG 3 cut(s) 121, 1129, 1632
VpaK11BI GGWCC 2 cut(s) 89, 568
XagI CCTNNNNNAGG 1 cut(s) 765
XapI RAATTY 3 cut(s) 679, 1100, 1132
XbaI TCTAGA 2 cut(s) 1318, 1345
XceI RCATGY 1 cut(s) 1655
XcmI CCANNNNNNNNNTGG 2 cut(s) 449, 1039
XmnI GAANNNNTTC 1 cut(s) 996
ZrmI AGTACT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.