Rh6BG017800

Endo-1,4-beta-xylanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004763
Physical Location & Seq
Forward (+)
2546795 .. 2549767
2973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG017800.1

Sequence Viewer

Length: 1668 bp
ATGGGAAACACTACTCTCACCTTCGTCAGGCTGCAGCTTCTTATGTTCATTCTCCTCATGGGCTCACCCTTTGTTTTTTCCTTTGATGGTCCACTATATGACTCTACTGCTTACACTGAGTGTAAAAGGCAGCCAGAGAAGCCTCTATATGGTGGAGGGATCATTGTTGATGAGCCTTCAGCTGTTGTAGACATAGACAATGGGGACGTTTATTCACCGGCATTCCTACTCCAAAATCTCACCGGCAACGGCACCATTTACAGCTTCTCCAGTTGGGTGAAGATAGGGGGTGCAGGTTCAGTACTGATACAGGCAAGTCTAAAGGCAGAAGATGAATCATACAATTGTATAGGAACTGTTTTGGCCAAGCGGGGATGCTGGTCATTCCTGAAGGGTGGCTTTGTCCTAAATTCACCACCAAATTTCTCTATACTATTCTTCCAGAATACGGATGGCAGAGATGTCGGCCTAGCAATTGCAAGTGCTTCTTTGCAGCCATTTAGTGTTCAGCAATGGAATACATATCACCAATATATGATCAACACTAAAAGGAAGCGTGCCGTGACGGTCCATGTCTCAAATGGACAAGGAAAGGGGCTGCAAGGAGCTGCAATTACCGTAGAGCAAGTCTCCAAAGATTTCCCATTTGGATCTGCAATTTCAAAGACCATTCTAGGCAATTTACCCTACCAGAATTGGTTTGTTGAGAGATTCAATGCTGCGGTGTTCGAGAATGAACTCAAGTGGTATGCAACAGAACCTAATCAAGGCAATGTCACGTACACTATAGCAGATCAAATGTTGCAATTTGTTCGAGCTCACCAAATCACTGCTAGAGGGCACAATATATTCTGGGAAGATCCCAAATACACACCCCCTTGGGTTCGATACCTCACAGCTGATCAGCTACAATCAGCTGTCAACTCTCGGATACAAAGCCTAATGAGCAAATACAAAGAAGAGTTCATTCATTGGGATGTCAGCAATGAAATGCTTCACTTTGATTTCTATGAGAAACAACTTGGCCCCAATGCCACCTTGGATTTCTTTCAGACGGCACACAAATCTGATCCTTTGGCAACCCTTTTTATGAATGACTTTAATGTGGTGGAGACTTGCACTGATGTAAATTCCACAGTTGATACCTATGTTTCAAGGCTGAGAGAACTTCAACAAGGTGGAGTATCAATGGATGGGATTGGACTAGAGGGTCATTTTACAATACCAAACCCCCCTCTAATGAGAGCTATTATAGACAAGTTGGCTACCCTAGGCCTTCCTATTTGGCTCACAGAGGTTGATATCAGCAATACTCTAGACAAAGAAACACAGGCCATTTATCTAGAGCAAGTATTAAGAGAAGGTTTCTCACATCCATCTGTAAATGGGATAATGCTCTGGAGTGCTCTACATCCTAACGGGTGCTACGAGATGTGCCTGACGGATAATAACCTTAAAAATCTACCAGCTGGTGATGTGGTGGACAAGCTCTTGAAAGAATGGCAAACCGGGAAGATAGAGGCTCAGATCGATGAGCATGGATCACATAGCTTTTTCGGGTTCTTGGGTGAGTACAGGGTTAGTGTGAAGTATGGAAATAGGACCGCAAACTCAACATTCTCACTGTGTGAAGGGGAAGAAACTAGACATGTTAGCATTCAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_10

Protein Analysis

555

Amino Acids

62.17

Weight (kDa)

5.3

Isoelectric Point (pI)

31.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 210 - 469 1e-47 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1209
Acc36I ACCTGC 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 189
AciI CCGC 3 cut(s) 370, 722, 1605
AclWI GGATC 5 cut(s) 167, 658, 854, 1064, 1549
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 3 cut(s) 409, 421, 1129
AcuI CTGAAG 2 cut(s) 162, 410
AdeI CACNNNGTG 2 cut(s) 120, 1628
AfaI GTAC 3 cut(s) 303, 782, 1574
AfiI CCNNNNNNNGG 4 cut(s) 27, 149, 448, 767
AflIII ACRYGT 1 cut(s) 1648
AgsI TTSAA 6 cut(s) 663, 715, 1155, 1172, 1495, 1661
Alw21I GWGCWC 2 cut(s) 820, 1408
Alw26I GTCTC 3 cut(s) 580, 634, 1106
AlwI GGATC 5 cut(s) 167, 658, 854, 1064, 1549
AoxI GGCC 5 cut(s) 363, 466, 1024, 1273, 1332
ApeKI GCWGC 7 cut(s) 31, 34, 130, 493, 598, 608, 719
ApoI RAATTY 3 cut(s) 409, 421, 1129
Asp700I GAANNNNTTC 1 cut(s) 993
AspA2I CCTAGG 1 cut(s) 1270
AspS9I GGNCC 4 cut(s) 89, 568, 1025, 1602
AsuC2I CCSGG 1 cut(s) 1510
AvaII GGWCC 3 cut(s) 89, 568, 1602
AvrII CCTAGG 1 cut(s) 1270
BaeGI GKGCMC 1 cut(s) 843
BaeI ACNNNNGTAYC 2 cut(s) 1134, 1167
BalI TGGCCA 1 cut(s) 365
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 2 cut(s) 65, 820
Bbv12I GWGCWC 2 cut(s) 820, 1408
BbvI GCAGC 7 cut(s) 18, 46, 142, 505, 585, 595, 706
BccI CCATC 4 cut(s) 80, 446, 1187, 1384
BceAI ACGGC 3 cut(s) 265, 545, 1071
BcgI CGANNNNNNTGC 4 cut(s) 445, 479, 794, 828
BciVI GTATCC 1 cut(s) 924
BclI TGATCA 2 cut(s) 537, 901
BcnI CCSGG 1 cut(s) 1510
BcoDI GTCTC 3 cut(s) 580, 634, 1106
BfaI CTAG 8 cut(s) 470, 674, 834, 1205, 1271, 1316, 1343, 1644
BfmI CTRYAG 2 cut(s) 32, 786
BfuAI ACCTGC 1 cut(s) 284
BfuI GTATCC 1 cut(s) 924
BisI GCNGC 7 cut(s) 32, 35, 131, 494, 599, 609, 720
BlnI CCTAGG 1 cut(s) 1270
BlsI GCNGC 7 cut(s) 33, 36, 132, 495, 600, 610, 721
BmcAI AGTACT 1 cut(s) 303
Bme1390I CCNGG 1 cut(s) 1510
Bme18I GGWCC 3 cut(s) 89, 568, 1602
BmgT120I GGNCC 4 cut(s) 89, 568, 1025, 1602
BmiI GGNNCC 2 cut(s) 253, 1027
BmrFI CCNGG 1 cut(s) 1510
BmsI GCATC 1 cut(s) 365
BplI GAGNNNNNCTC 2 cut(s) 614, 646
BpmI CTGGAG 2 cut(s) 253, 1420
BpuEI CTTGAG 1 cut(s) 725
BpuMI CCSGG 1 cut(s) 1510
Bsa29I ATCGAT 1 cut(s) 1530
BsaAI YACGTR 1 cut(s) 780
BsaJI CCNNGG 3 cut(s) 878, 1038, 1270
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 149, 448, 767
Bse118I RCCGGY 2 cut(s) 217, 242
Bse1I ACTGG 1 cut(s) 270
Bse3DI GCAATG 3 cut(s) 518, 778, 991
BseCI ATCGAT 1 cut(s) 1530
BseDI CCNNGG 3 cut(s) 878, 1038, 1270
BseGI GGATG 6 cut(s) 380, 457, 982, 1198, 1372, 1411
BseLI CCNNNNNNNGG 4 cut(s) 27, 149, 448, 767
BseMI GCAATG 3 cut(s) 518, 778, 991
BseMII CTCAG 3 cut(s) 108, 1151, 1538
BseNI ACTGG 1 cut(s) 270
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 843
BseXI GCAGC 7 cut(s) 18, 46, 142, 505, 585, 595, 706
BsgI GTGCAG 1 cut(s) 312
BshFI GGCC 5 cut(s) 365, 468, 1026, 1275, 1334
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 1530
BsiHKAI GWGCWC 2 cut(s) 820, 1408
BsiSI CCGG 3 cut(s) 218, 243, 1509
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 4 cut(s) 27, 149, 448, 767
BsmAI GTCTC 3 cut(s) 580, 634, 1106
BsmFI GGGAC 1 cut(s) 218
BsmI GAATGC 2 cut(s) 221, 1656
BsnI GGCC 5 cut(s) 365, 468, 1026, 1275, 1334
Bsp1286I GDGCHC 4 cut(s) 65, 820, 843, 1408
Bsp143I GATC 9 cut(s) 159, 537, 650, 793, 859, 901, 1069, 1527, 1541
BspACI CCGC 3 cut(s) 370, 722, 1605
BspANI GGCC 5 cut(s) 365, 468, 1026, 1275, 1334
BspCNI CTCAG 3 cut(s) 109, 1152, 1537
BspDI ATCGAT 1 cut(s) 1530
BspLI GGNNCC 2 cut(s) 253, 1027
BspMAI CTGCAG 1 cut(s) 36
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 5 cut(s) 167, 658, 854, 1064, 1549
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 3 cut(s) 518, 778, 991
BsrFI RCCGGY 2 cut(s) 217, 242
BsrI ACTGG 1 cut(s) 270
BssAI RCCGGY 2 cut(s) 217, 242
BssECI CCNNGG 3 cut(s) 878, 1038, 1270
BssMI GATC 9 cut(s) 159, 537, 650, 793, 859, 901, 1069, 1527, 1541
BssT1I CCWWGG 3 cut(s) 878, 1038, 1270
Bst4CI ACNGT 6 cut(s) 358, 568, 619, 1138, 1626, 1665
Bst6I CTCTTC 1 cut(s) 954
BstBAI YACGTR 1 cut(s) 780
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 3 cut(s) 117, 1160, 1524
BstENI CCTNNNNNAGG 2 cut(s) 25, 765
BstF5I GGATG 6 cut(s) 380, 457, 982, 1198, 1372, 1411
BstKTI GATC 9 cut(s) 162, 540, 653, 796, 862, 904, 1072, 1530, 1544
BstMAI GTCTC 3 cut(s) 580, 634, 1106
BstMBI GATC 9 cut(s) 159, 537, 650, 793, 859, 901, 1069, 1527, 1541
BstMWI GCNNNNNNNGC 2 cut(s) 139, 945
BstNSI RCATGY 1 cut(s) 1652
BstSCI CCNGG 1 cut(s) 1508
BstSFI CTRYAG 2 cut(s) 32, 786
BstSLI GKGCMC 1 cut(s) 843
BstV1I GCAGC 7 cut(s) 18, 46, 142, 505, 585, 595, 706
BstX2I RGATCY 2 cut(s) 650, 859
BstYI RGATCY 2 cut(s) 650, 859
Bsu15I ATCGAT 1 cut(s) 1530
BsuI GTATCC 1 cut(s) 924
BsuRI GGCC 5 cut(s) 365, 468, 1026, 1275, 1334
BsuTUI ATCGAT 1 cut(s) 1530
BtsCI GGATG 6 cut(s) 380, 457, 982, 1198, 1372, 1411
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 4 cut(s) 114, 828, 1119, 1622
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 558
Cfr10I RCCGGY 2 cut(s) 217, 242
Cfr13I GGNCC 4 cut(s) 89, 568, 1025, 1602
ClaI ATCGAT 1 cut(s) 1530
Csp6I GTAC 3 cut(s) 302, 781, 1573
CviAII CATG 4 cut(s) 58, 572, 1538, 1649
CviQI GTAC 3 cut(s) 302, 781, 1573
DdeI CTNAG 3 cut(s) 117, 1160, 1524
DpnI GATC 9 cut(s) 161, 539, 652, 795, 861, 903, 1071, 1529, 1543
DpnII GATC 9 cut(s) 159, 537, 650, 793, 859, 901, 1069, 1527, 1541
DraIII CACNNNGTG 2 cut(s) 120, 1628
DrdI GACNNNNNNGTC 1 cut(s) 1209
DseDI GACNNNNNNGTC 1 cut(s) 1209
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 954
EarI CTCTTC 1 cut(s) 954
Ecl136II GAGCTC 1 cut(s) 818
Eco130I CCWWGG 3 cut(s) 878, 1038, 1270
Eco147I AGGCCT 1 cut(s) 1275
Eco24I GRGCYC 2 cut(s) 65, 820
Eco32I GATATC 1 cut(s) 1303
Eco47I GGWCC 3 cut(s) 89, 568, 1602
Eco53kI GAGCTC 1 cut(s) 818
Eco57I CTGAAG 2 cut(s) 162, 410
EcoICRI GAGCTC 1 cut(s) 818
EcoNI CCTNNNNNAGG 2 cut(s) 25, 765
EcoRV GATATC 1 cut(s) 1303
EcoT14I CCWWGG 3 cut(s) 878, 1038, 1270
EcoT38I GRGCYC 2 cut(s) 65, 820
ErhI CCWWGG 3 cut(s) 878, 1038, 1270
FaeI CATG 4 cut(s) 61, 575, 1541, 1652
FalI AAGNNNNNCTT 4 cut(s) 383, 415, 472, 504
FaqI GGGAC 1 cut(s) 218
FatI CATG 4 cut(s) 57, 571, 1537, 1648
FauI CCCGC 1 cut(s) 363
FbaI TGATCA 2 cut(s) 537, 901
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 7 cut(s) 32, 35, 131, 494, 599, 609, 720
FokI GGATG 6 cut(s) 387, 464, 989, 1205, 1359, 1398
FriOI GRGCYC 2 cut(s) 65, 820
Fsp4HI GCNGC 7 cut(s) 32, 35, 131, 494, 599, 609, 720
FspBI CTAG 8 cut(s) 470, 674, 834, 1205, 1271, 1316, 1343, 1644
GluI GCNGC 7 cut(s) 32, 35, 131, 494, 599, 609, 720
GsuI CTGGAG 2 cut(s) 253, 1420
HaeIII GGCC 5 cut(s) 365, 468, 1026, 1275, 1334
HapII CCGG 3 cut(s) 218, 243, 1509
Hin1II CATG 4 cut(s) 61, 575, 1541, 1652
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HinfI GANTC 3 cut(s) 101, 335, 711
HpaII CCGG 3 cut(s) 218, 243, 1509
Hpy166II GTNNAC 5 cut(s) 92, 190, 783, 922, 1483
Hpy188I TCNGA 4 cut(s) 930, 1053, 1069, 1527
Hpy188III TCNNGA 7 cut(s) 388, 442, 730, 1316, 1343, 1399, 1492
Hpy8I GTNNAC 5 cut(s) 92, 190, 783, 922, 1483
HpyAV CCTTC 6 cut(s) 31, 186, 385, 1286, 1355, 1625
HpyCH4III ACNGT 6 cut(s) 358, 568, 619, 1138, 1626, 1665
HpyCH4IV ACGT 2 cut(s) 207, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 945
HpyF3I CTNAG 3 cut(s) 117, 1160, 1524
HpySE526I ACGT 2 cut(s) 207, 779
Hsp92II CATG 4 cut(s) 61, 575, 1541, 1652
Ksp22I TGATCA 2 cut(s) 537, 901
Kzo9I GATC 9 cut(s) 159, 537, 650, 793, 859, 901, 1069, 1527, 1541
LmnI GCTCC 1 cut(s) 605
Lsp1109I GCAGC 7 cut(s) 18, 46, 142, 505, 585, 595, 706
LweI GCATC 1 cut(s) 365
MaeI CTAG 8 cut(s) 470, 674, 834, 1205, 1271, 1316, 1343, 1644
MaeII ACGT 2 cut(s) 207, 779
MaeIII GTNAC 2 cut(s) 562, 775
MalI GATC 9 cut(s) 161, 539, 652, 795, 861, 903, 1071, 1529, 1543
MboI GATC 9 cut(s) 159, 537, 650, 793, 859, 901, 1069, 1527, 1541
MboII GAAGA 7 cut(s) 292, 341, 430, 869, 971, 1525, 1649
MfeI CAATTG 2 cut(s) 343, 474
MflI RGATCY 2 cut(s) 650, 859
MhlI GDGCHC 4 cut(s) 65, 820, 843, 1408
MlsI TGGCCA 1 cut(s) 365
MluNI TGGCCA 1 cut(s) 365
MlyI GAGTC 1 cut(s) 95
MnlI CCTC 9 cut(s) 65, 149, 153, 830, 902, 1201, 1245, 1288, 1513
Mox20I TGGCCA 1 cut(s) 365
MroXI GAANNNNTTC 1 cut(s) 993
MscI TGGCCA 1 cut(s) 365
MseI TTAA 3 cut(s) 1101, 1355, 1455
MslI CAYNNNNRTG 1 cut(s) 975
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 4 cut(s) 182, 899, 917, 1469
MspI CCGG 3 cut(s) 218, 243, 1509
MspR9I CCNGG 1 cut(s) 1510
MunI CAATTG 2 cut(s) 343, 474
Mva1269I GAATGC 2 cut(s) 221, 1656
MwoI GCNNNNNNNGC 2 cut(s) 139, 945
NciI CCSGG 1 cut(s) 1510
NdeII GATC 9 cut(s) 159, 537, 650, 793, 859, 901, 1069, 1527, 1541
NlaIII CATG 4 cut(s) 61, 575, 1541, 1652
NlaIV GGNNCC 2 cut(s) 253, 1027
NmuCI GTSAC 2 cut(s) 562, 775
NspI RCATGY 1 cut(s) 1652
PceI AGGCCT 1 cut(s) 1275
PciI ACATGT 1 cut(s) 1648
PcsI WCGNNNNNNNCGW 1 cut(s) 1425
PctI GAATGC 2 cut(s) 221, 1656
PdmI GAANNNNTTC 1 cut(s) 993
PfeI GAWTC 2 cut(s) 335, 711
PkrI GCNGC 7 cut(s) 33, 36, 132, 495, 600, 610, 721
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Ppu21I YACGTR 1 cut(s) 780
PscI ACATGT 1 cut(s) 1648
Psp124BI GAGCTC 1 cut(s) 820
PspN4I GGNNCC 2 cut(s) 253, 1027
PspPI GGNCC 4 cut(s) 89, 568, 1025, 1602
PstI CTGCAG 1 cut(s) 36
PsuI RGATCY 2 cut(s) 650, 859
PvuII CAGCTG 4 cut(s) 182, 899, 917, 1469
RsaI GTAC 3 cut(s) 303, 782, 1574
RsaNI GTAC 3 cut(s) 302, 781, 1573
RseI CAYNNNNRTG 1 cut(s) 975
SacI GAGCTC 1 cut(s) 820
SaqAI TTAA 3 cut(s) 1101, 1355, 1455
SatI GCNGC 7 cut(s) 32, 35, 131, 494, 599, 609, 720
Sau3AI GATC 9 cut(s) 159, 537, 650, 793, 859, 901, 1069, 1527, 1541
Sau96I GGNCC 4 cut(s) 89, 568, 1025, 1602
ScaI AGTACT 1 cut(s) 303
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 1 cut(s) 1510
SduI GDGCHC 4 cut(s) 65, 820, 843, 1408
SfaNI GCATC 1 cut(s) 365
SfcI CTRYAG 2 cut(s) 32, 786
SinI GGWCC 3 cut(s) 89, 568, 1602
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
SseBI AGGCCT 1 cut(s) 1275
SsiI CCGC 3 cut(s) 370, 722, 1605
SspMI CTAG 8 cut(s) 470, 674, 834, 1205, 1271, 1316, 1343, 1644
SstI GAGCTC 1 cut(s) 820
StuI AGGCCT 1 cut(s) 1275
StyD4I CCNGG 1 cut(s) 1508
StyI CCWWGG 3 cut(s) 878, 1038, 1270
TaaI ACNGT 6 cut(s) 358, 568, 619, 1138, 1626, 1665
TaiI ACGT 2 cut(s) 210, 782
TaqI TCGA 4 cut(s) 729, 814, 886, 1530
TatI WGTACW 2 cut(s) 301, 1572
TfiI GAWTC 2 cut(s) 335, 711
Tru1I TTAA 3 cut(s) 1101, 1355, 1455
Tru9I TTAA 3 cut(s) 1101, 1355, 1455
TscAI CASTG 4 cut(s) 121, 835, 1126, 1629
TseFI GTSAC 2 cut(s) 562, 775
TseI GCWGC 7 cut(s) 31, 34, 130, 493, 598, 608, 719
Tsp45I GTSAC 2 cut(s) 562, 775
TspDTI ATGAA 7 cut(s) 37, 348, 750, 955, 959, 1002, 1106
TspGWI ACGGA 2 cut(s) 464, 1457
TspRI CASTG 4 cut(s) 121, 835, 1126, 1629
VpaK11BI GGWCC 3 cut(s) 89, 568, 1602
XagI CCTNNNNNAGG 2 cut(s) 25, 765
XapI RAATTY 3 cut(s) 409, 421, 1129
XbaI TCTAGA 2 cut(s) 1315, 1342
XceI RCATGY 1 cut(s) 1652
XcmI CCANNNNNNNNNTGG 3 cut(s) 449, 578, 1036
XmaJI CCTAGG 1 cut(s) 1270
XmiI GTMKAC 1 cut(s) 189
XmnI GAANNNNTTC 1 cut(s) 993
XspI CTAG 8 cut(s) 470, 674, 834, 1205, 1271, 1316, 1343, 1644
ZrmI AGTACT 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.