Rh6AG022100

Endo-1,4-beta-xylanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0004763
Physical Location & Seq
Forward (+)
2441921 .. 2445273
3353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG022100.1

Sequence Viewer

Length: 1680 bp
ATGGGTGTTGCAGATGGTCCACTATATGACTCTACTGCTTACACTGAGGTGAAAGAGAACTCATTTTTCCCAGATCAATTTCGTTTGAGTTGCACAGTAACTAATTTTACACTCGTTGATTGTTTTCGACAGTGTAAAAGGCAGCCAGAGAAGCCTCTATATGGTGGAGGGATCATTGTTGATGAGCCTTCAGCTGTTGTAGACATAGACAATGGGGACGTTTATTCACCGGCATTCCTACTCCAAAATCTCACCGGCAACGGCACCATTTACAGCTTCTCCAGTTGGGTGAAGATAGGGGGTGCAGGTTCAGCACTGATACACGCAAGTCTAAAGGCAGAAGATGAATCATACAATTGTATAGGAACTGTTTTGGCCAAGCGGGGATGCTGGTCATTCCTCAAGGGTGGCTTTGTCCTAAATTCACCACCTAATTTCTCTATACTATTCTTCCAGAATACGGATGGCAGAGATGTCGGCCTAGCAATTGCAAGTGCTTCTTTGCAGCCATTTAGTGTTCAGCAATGGAATACATATCACCAATATATGATCAACACTAAAAGGAAGCGTGCCGTGATGGTCCATGTCTCAAATGGACAAGGAAAGGGGCTGCAAGGAGCTGCAATTACCGTAGAGCAAGTCTCCAAAGATTTCCCATTTGGATCTGCAATTTCAAAGACCATTCTAGGCAATTTACCCTACCAGAATTGGTTTGTTGAGAGATTCAATGCTGCGGTGTTCGAGAATGAACTCAAGTGGTATGCAACAGAACCTAATCAAGGCAATGTCACTTACAATATAGCAGATCAAATGTTGCAATTTGTTCGAGCTCACCAAATCACTGCTAGAGGGCACAATATATTCTGGGAAGATCCCAAATACACACCACCTTGGGTTCGATACCTCACAGCTGATCAGCTACAATCAGCTGTCAACTCTCGGATACAAAGCCTAATGAGCAAATACAAAGAAGAGTTCATTCATTGGGATGTCAGCAATGAAATGCTTCACTTTGATTTCTATGAGAAACAGCTTGGCCCCAATGCCACCTTGGATTTCTTTCAGACGGCACACAAATCTGATCCTTTGGCAACCCTTTTTATGAATGACTTTAATGTGGTGGAGACTTGCACTGATGTGAATTCCACAGTTGATACCTATGTTTCAAGGCTGAGAGAACTTCAACAAGGTGGAGTATCAATGGATGGGATTGGACTAGAGGGTCATTTTACAATACCAAACCCCCCTCTAATGAGAGCTATTATAGACAAGTTGGCTACCCTAGGCCTTCCTATTTGGCTCACAGAGGTTGATATCAGCAATACTCTAGACAAAGAAACACAGGCCATTTATCTAGAGCAAGTATTAAGAGAAGGTTTCTCACATCCATCTGTAAATGGGATAATGCTCTGGAGTGCTCTACATCCTAACGGGTGCTACGAGATGTGCCTGACGGATAATAACCTTAAAAATCTACCAGCTGGTGATGTGGTGGACAAGCTCTTGAAAGAATGGCAAACCGGGAAGATAGAGGCTCAGATCGATGAGCATGGATCACATAGCTTTTTCGGGTTCTTGGGTGAGTACAGGGTTAGTGTGAAGTATGGAAATAGGACCGCAAACTCAACATTCTCACTGTGTGAAGGGAAAGAAACTAGACATGTTAGCATTCAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_10

Protein Analysis

559

Amino Acids

62.67

Weight (kDa)

5.43

Isoelectric Point (pI)

29.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 214 - 473 1.1e-47 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1221
Acc36I ACCTGC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 263
AccI GTMKAC 1 cut(s) 201
AciI CCGC 3 cut(s) 382, 734, 1617
AclWI GGATC 5 cut(s) 179, 670, 866, 1076, 1561
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 2 cut(s) 421, 1141
AcuI CTGAAG 1 cut(s) 174
AdeI CACNNNGTG 1 cut(s) 1640
AfaI GTAC 1 cut(s) 1586
AfiI CCNNNNNNNGG 3 cut(s) 161, 460, 779
AflIII ACRYGT 1 cut(s) 1660
AgsI TTSAA 6 cut(s) 675, 727, 1167, 1184, 1507, 1673
AleI CACNNNNGTG 2 cut(s) 47, 1136
Alw21I GWGCWC 2 cut(s) 832, 1420
Alw26I GTCTC 3 cut(s) 592, 646, 1118
AlwI GGATC 5 cut(s) 179, 670, 866, 1076, 1561
AoxI GGCC 5 cut(s) 375, 478, 1036, 1285, 1344
ApeKI GCWGC 5 cut(s) 142, 505, 610, 620, 731
ApoI RAATTY 2 cut(s) 421, 1141
Asp700I GAANNNNTTC 1 cut(s) 1005
AspA2I CCTAGG 1 cut(s) 1282
AspS9I GGNCC 4 cut(s) 17, 580, 1037, 1614
AsuC2I CCSGG 1 cut(s) 1522
AsuHPI GGTGA 9 cut(s) 61, 219, 244, 301, 417, 530, 824, 1496, 1592
AvaII GGWCC 3 cut(s) 17, 580, 1614
AvrII CCTAGG 1 cut(s) 1282
BaeGI GKGCMC 1 cut(s) 855
BaeI ACNNNNGTAYC 2 cut(s) 1146, 1179
BalI TGGCCA 1 cut(s) 377
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 832
Bbv12I GWGCWC 2 cut(s) 832, 1420
BbvI GCAGC 5 cut(s) 154, 517, 597, 607, 718
BccI CCATC 5 cut(s) 8, 458, 571, 1199, 1396
BceAI ACGGC 3 cut(s) 277, 557, 1083
BcgI CGANNNNNNTGC 4 cut(s) 457, 491, 806, 840
BciVI GTATCC 1 cut(s) 936
BclI TGATCA 2 cut(s) 549, 913
BcnI CCSGG 1 cut(s) 1522
BcoDI GTCTC 3 cut(s) 592, 646, 1118
BfaI CTAG 8 cut(s) 482, 686, 846, 1217, 1283, 1328, 1355, 1656
BfuAI ACCTGC 1 cut(s) 296
BfuI GTATCC 1 cut(s) 936
BisI GCNGC 5 cut(s) 143, 506, 611, 621, 732
BlnI CCTAGG 1 cut(s) 1282
BlsI GCNGC 5 cut(s) 144, 507, 612, 622, 733
Bme1390I CCNGG 1 cut(s) 1522
Bme18I GGWCC 3 cut(s) 17, 580, 1614
BmgT120I GGNCC 4 cut(s) 17, 580, 1037, 1614
BmiI GGNNCC 2 cut(s) 265, 1039
BmrFI CCNGG 1 cut(s) 1522
BmsI GCATC 1 cut(s) 377
BplI GAGNNNNNCTC 2 cut(s) 626, 658
BpmI CTGGAG 2 cut(s) 265, 1432
BpuEI CTTGAG 2 cut(s) 386, 737
BpuMI CCSGG 1 cut(s) 1522
Bsa29I ATCGAT 1 cut(s) 1542
BsaJI CCNNGG 3 cut(s) 890, 1050, 1282
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 460, 779
Bse118I RCCGGY 2 cut(s) 229, 254
Bse1I ACTGG 1 cut(s) 282
Bse3DI GCAATG 3 cut(s) 530, 790, 1003
BseCI ATCGAT 1 cut(s) 1542
BseDI CCNNGG 3 cut(s) 890, 1050, 1282
BseGI GGATG 6 cut(s) 392, 469, 994, 1210, 1384, 1423
BseLI CCNNNNNNNGG 3 cut(s) 161, 460, 779
BseMI GCAATG 3 cut(s) 530, 790, 1003
BseMII CTCAG 3 cut(s) 36, 1163, 1550
BseNI ACTGG 1 cut(s) 282
BseSI GKGCMC 1 cut(s) 855
BseXI GCAGC 5 cut(s) 154, 517, 597, 607, 718
BsgI GTGCAG 1 cut(s) 324
BshFI GGCC 5 cut(s) 377, 480, 1038, 1287, 1346
BshNI GGYRCC 1 cut(s) 263
BshVI ATCGAT 1 cut(s) 1542
BsiHKAI GWGCWC 2 cut(s) 832, 1420
BsiSI CCGG 3 cut(s) 230, 255, 1521
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 3 cut(s) 161, 460, 779
BsmAI GTCTC 3 cut(s) 592, 646, 1118
BsmFI GGGAC 1 cut(s) 230
BsmI GAATGC 2 cut(s) 233, 1668
BsnI GGCC 5 cut(s) 377, 480, 1038, 1287, 1346
Bsp1286I GDGCHC 3 cut(s) 832, 855, 1420
BspACI CCGC 3 cut(s) 382, 734, 1617
BspANI GGCC 5 cut(s) 377, 480, 1038, 1287, 1346
BspCNI CTCAG 3 cut(s) 37, 1164, 1549
BspDI ATCGAT 1 cut(s) 1542
BspLI GGNNCC 2 cut(s) 265, 1039
BspMI ACCTGC 1 cut(s) 296
BspPI GGATC 5 cut(s) 179, 670, 866, 1076, 1561
BspT107I GGYRCC 1 cut(s) 263
BsrDI GCAATG 3 cut(s) 530, 790, 1003
BsrFI RCCGGY 2 cut(s) 229, 254
BsrI ACTGG 1 cut(s) 282
BssAI RCCGGY 2 cut(s) 229, 254
BssECI CCNNGG 3 cut(s) 890, 1050, 1282
BssT1I CCWWGG 3 cut(s) 890, 1050, 1282
Bst4CI ACNGT 7 cut(s) 97, 132, 370, 631, 1150, 1638, 1677
Bst6I CTCTTC 1 cut(s) 966
BstC8I GCNNGC 1 cut(s) 570
BstDEI CTNAG 3 cut(s) 45, 1172, 1536
BstENI CCTNNNNNAGG 1 cut(s) 777
BstF5I GGATG 6 cut(s) 392, 469, 994, 1210, 1384, 1423
BstMAI GTCTC 3 cut(s) 592, 646, 1118
BstMWI GCNNNNNNNGC 3 cut(s) 151, 311, 957
BstNSI RCATGY 1 cut(s) 1664
BstSCI CCNGG 1 cut(s) 1520
BstSLI GKGCMC 1 cut(s) 855
BstV1I GCAGC 5 cut(s) 154, 517, 597, 607, 718
BstX2I RGATCY 2 cut(s) 662, 871
BstYI RGATCY 2 cut(s) 662, 871
Bsu15I ATCGAT 1 cut(s) 1542
BsuI GTATCC 1 cut(s) 936
BsuRI GGCC 5 cut(s) 377, 480, 1038, 1287, 1346
BsuTUI ATCGAT 1 cut(s) 1542
BtsCI GGATG 6 cut(s) 392, 469, 994, 1210, 1384, 1423
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 6 cut(s) 42, 137, 314, 840, 1131, 1634
BveI ACCTGC 1 cut(s) 296
Cac8I GCNNGC 1 cut(s) 570
Cfr10I RCCGGY 2 cut(s) 229, 254
Cfr13I GGNCC 4 cut(s) 17, 580, 1037, 1614
ClaI ATCGAT 1 cut(s) 1542
Csp6I GTAC 1 cut(s) 1585
CviAII CATG 3 cut(s) 584, 1550, 1661
CviQI GTAC 1 cut(s) 1585
DdeI CTNAG 3 cut(s) 45, 1172, 1536
DraIII CACNNNGTG 1 cut(s) 1640
DrdI GACNNNNNNGTC 1 cut(s) 1221
DseDI GACNNNNNNGTC 1 cut(s) 1221
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 966
EarI CTCTTC 1 cut(s) 966
Ecl136II GAGCTC 1 cut(s) 830
Eco130I CCWWGG 3 cut(s) 890, 1050, 1282
Eco147I AGGCCT 1 cut(s) 1287
Eco24I GRGCYC 1 cut(s) 832
Eco32I GATATC 1 cut(s) 1315
Eco47I GGWCC 3 cut(s) 17, 580, 1614
Eco53kI GAGCTC 1 cut(s) 830
Eco57I CTGAAG 1 cut(s) 174
EcoICRI GAGCTC 1 cut(s) 830
EcoNI CCTNNNNNAGG 1 cut(s) 777
EcoRI GAATTC 1 cut(s) 1141
EcoRV GATATC 1 cut(s) 1315
EcoT14I CCWWGG 3 cut(s) 890, 1050, 1282
EcoT38I GRGCYC 1 cut(s) 832
ErhI CCWWGG 3 cut(s) 890, 1050, 1282
FaeI CATG 3 cut(s) 587, 1553, 1664
FalI AAGNNNNNCTT 4 cut(s) 395, 427, 484, 516
FaqI GGGAC 1 cut(s) 230
FatI CATG 3 cut(s) 583, 1549, 1660
FauI CCCGC 1 cut(s) 375
FbaI TGATCA 2 cut(s) 549, 913
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 5 cut(s) 143, 506, 611, 621, 732
FokI GGATG 6 cut(s) 399, 476, 1001, 1217, 1371, 1410
FriOI GRGCYC 1 cut(s) 832
Fsp4HI GCNGC 5 cut(s) 143, 506, 611, 621, 732
FspBI CTAG 8 cut(s) 482, 686, 846, 1217, 1283, 1328, 1355, 1656
GluI GCNGC 5 cut(s) 143, 506, 611, 621, 732
GsuI CTGGAG 2 cut(s) 265, 1432
HaeIII GGCC 5 cut(s) 377, 480, 1038, 1287, 1346
HapII CCGG 3 cut(s) 230, 255, 1521
Hin1II CATG 3 cut(s) 587, 1553, 1664
HincII GTYRAC 1 cut(s) 934
HindII GTYRAC 1 cut(s) 934
HinfI GANTC 3 cut(s) 29, 347, 723
HpaII CCGG 3 cut(s) 230, 255, 1521
HphI GGTGA 9 cut(s) 61, 219, 244, 301, 417, 530, 824, 1496, 1592
Hpy166II GTNNAC 4 cut(s) 20, 202, 934, 1495
Hpy188I TCNGA 4 cut(s) 942, 1065, 1081, 1539
Hpy188III TCNNGA 6 cut(s) 454, 742, 1328, 1355, 1411, 1504
Hpy8I GTNNAC 4 cut(s) 20, 202, 934, 1495
HpyAV CCTTC 4 cut(s) 198, 1298, 1367, 1637
HpyCH4III ACNGT 7 cut(s) 97, 132, 370, 631, 1150, 1638, 1677
HpyCH4IV ACGT 1 cut(s) 219
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 311, 957
HpyF3I CTNAG 3 cut(s) 45, 1172, 1536
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 3 cut(s) 587, 1553, 1664
Ksp22I TGATCA 2 cut(s) 549, 913
LmnI GCTCC 1 cut(s) 617
Lsp1109I GCAGC 5 cut(s) 154, 517, 597, 607, 718
LweI GCATC 1 cut(s) 377
MaeI CTAG 8 cut(s) 482, 686, 846, 1217, 1283, 1328, 1355, 1656
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 2 cut(s) 97, 787
MboII GAAGA 6 cut(s) 304, 353, 442, 881, 983, 1537
MfeI CAATTG 2 cut(s) 355, 486
MflI RGATCY 2 cut(s) 662, 871
MhlI GDGCHC 3 cut(s) 832, 855, 1420
MlsI TGGCCA 1 cut(s) 377
MluNI TGGCCA 1 cut(s) 377
MlyI GAGTC 1 cut(s) 23
Mox20I TGGCCA 1 cut(s) 377
MroXI GAANNNNTTC 1 cut(s) 1005
MscI TGGCCA 1 cut(s) 377
MseI TTAA 3 cut(s) 1113, 1367, 1467
MslI CAYNNNNRTG 3 cut(s) 47, 987, 1136
Msp20I TGGCCA 1 cut(s) 377
MspA1I CMGCKG 4 cut(s) 194, 911, 929, 1481
MspI CCGG 3 cut(s) 230, 255, 1521
MspR9I CCNGG 1 cut(s) 1522
MunI CAATTG 2 cut(s) 355, 486
Mva1269I GAATGC 2 cut(s) 233, 1668
MwoI GCNNNNNNNGC 3 cut(s) 151, 311, 957
NciI CCSGG 1 cut(s) 1522
NlaIII CATG 3 cut(s) 587, 1553, 1664
NlaIV GGNNCC 2 cut(s) 265, 1039
NmuCI GTSAC 1 cut(s) 787
NspI RCATGY 1 cut(s) 1664
OliI CACNNNNGTG 2 cut(s) 47, 1136
PceI AGGCCT 1 cut(s) 1287
PciI ACATGT 1 cut(s) 1660
PcsI WCGNNNNNNNCGW 1 cut(s) 1437
PctI GAATGC 2 cut(s) 233, 1668
PdmI GAANNNNTTC 1 cut(s) 1005
PfeI GAWTC 2 cut(s) 347, 723
PkrI GCNGC 5 cut(s) 144, 507, 612, 622, 733
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PscI ACATGT 1 cut(s) 1660
Psp124BI GAGCTC 1 cut(s) 832
PspN4I GGNNCC 2 cut(s) 265, 1039
PspPI GGNCC 4 cut(s) 17, 580, 1037, 1614
PsuI RGATCY 2 cut(s) 662, 871
PvuII CAGCTG 4 cut(s) 194, 911, 929, 1481
RsaI GTAC 1 cut(s) 1586
RsaNI GTAC 1 cut(s) 1585
RseI CAYNNNNRTG 3 cut(s) 47, 987, 1136
SacI GAGCTC 1 cut(s) 832
SaqAI TTAA 3 cut(s) 1113, 1367, 1467
SatI GCNGC 5 cut(s) 143, 506, 611, 621, 732
Sau96I GGNCC 4 cut(s) 17, 580, 1037, 1614
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 1 cut(s) 1522
SduI GDGCHC 3 cut(s) 832, 855, 1420
SfaNI GCATC 1 cut(s) 377
SinI GGWCC 3 cut(s) 17, 580, 1614
SmiMI CAYNNNNRTG 3 cut(s) 47, 987, 1136
SmlI CTYRAG 2 cut(s) 401, 752
SmoI CTYRAG 2 cut(s) 401, 752
SseBI AGGCCT 1 cut(s) 1287
SsiI CCGC 3 cut(s) 382, 734, 1617
SspMI CTAG 8 cut(s) 482, 686, 846, 1217, 1283, 1328, 1355, 1656
SstI GAGCTC 1 cut(s) 832
StuI AGGCCT 1 cut(s) 1287
StyD4I CCNGG 1 cut(s) 1520
StyI CCWWGG 3 cut(s) 890, 1050, 1282
TaaI ACNGT 7 cut(s) 97, 132, 370, 631, 1150, 1638, 1677
TaiI ACGT 1 cut(s) 222
TaqI TCGA 5 cut(s) 127, 741, 826, 898, 1542
TatI WGTACW 1 cut(s) 1584
TfiI GAWTC 2 cut(s) 347, 723
Tru1I TTAA 3 cut(s) 1113, 1367, 1467
Tru9I TTAA 3 cut(s) 1113, 1367, 1467
TscAI CASTG 6 cut(s) 49, 137, 321, 847, 1138, 1641
TseFI GTSAC 1 cut(s) 787
TseI GCWGC 5 cut(s) 142, 505, 610, 620, 731
Tsp45I GTSAC 1 cut(s) 787
TspDTI ATGAA 6 cut(s) 360, 762, 967, 971, 1014, 1118
TspGWI ACGGA 2 cut(s) 476, 1469
TspRI CASTG 6 cut(s) 49, 137, 321, 847, 1138, 1641
VpaK11BI GGWCC 3 cut(s) 17, 580, 1614
XagI CCTNNNNNAGG 1 cut(s) 777
XapI RAATTY 2 cut(s) 421, 1141
XbaI TCTAGA 2 cut(s) 1327, 1354
XceI RCATGY 1 cut(s) 1664
XcmI CCANNNNNNNNNTGG 3 cut(s) 461, 590, 1048
XmaJI CCTAGG 1 cut(s) 1282
XmiI GTMKAC 1 cut(s) 201
XmnI GAANNNNTTC 1 cut(s) 1005
XspI CTAG 8 cut(s) 482, 686, 846, 1217, 1283, 1328, 1355, 1656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.