MD10G1122000.v1.1

Endo-1,4-beta-xylanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004763
Physical Location & Seq
Reverse (-)
19934685 .. 19937880
3196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1122000.v1.1.491

Sequence Viewer

Length: 1422 bp
ATGTTTATCTTGTCCAATCTATTTAGTTGGGTCAAGATAGAGGGTGCAGGCTCAGCTCTCATAAGGGCAAGCCTAAAGACAGAAAATGAAACAAATAAGTGTGTAGGAACTGTTTTGGCTAAGCATGGATGCTGGTCATTTCTCAAGGGTGGCTTTGTCCTAAACTCACCATCCCAATTATCCACCTTATTCTTTCAGAATGCAGATGACAGAGATGTCAACATTGAAATTACCAGCTCTTCTTTGCAGCCATTTAGTGTTCAACAATGGAGCACGTATCAGCAATATATGATCAACACTAAACGGAAGCGTGCCGTGACGGTTCATGTCTCAGATAAGCAAGGAAAGAGGCTGCAAGGATTGGAAATTGACATAGAGCAAGTCTCCAAAGATTTCCCATTTGGATCTGCAATTGCAAAGACCATTCTAGGCAATTTGCCCTATCAGGATTGGTTCGCAAAACGATTCAATGCAGCGGTGTTTGAGAACGAACTCAAGTGGTACGCGACGGAGCCAGAACAGGGCAAAACCAACTACACCATTGCAGATCAAATGCTGCAATTTGTGCGCGCCAACCAAGTCATTGCTAGAGGTCACAACATATTCTGGGAAGACCCCAAATACACGCCGCAATGGGTTCGAAACCTCACCGGCAGCGACCTGCAATCTGCCGTAAACTCCAGAATCCAAAGCCTAATGAGCAAGTACAGAGAAGAGTTCGTCCATTGGGACGTCAGCAACGAAATGCTTCACTTTGATTTCTACGAGCAGAAGCTTGGCCCCAACGCCACATTGCATTTCTTTGAGACGGCACACAAATCTGACCCTTTGGCAACCCTTTTCATGAATGATTTTAATGTGGTGGAGACTTGCAGTGATGTGAATTCGACCGTTGATTCCTACATTTCAAGGTTGAGAGAGCTTAGGCATGGTGGAGCATTGATGGATGGGATTGGACTGGAGGGTCATTTTACAGTACCAAACCTCCCTCTAATGAGAGCAATCCTTGACAAGTTGGCCACATTAGGCCTTCCCATTTGGCTTACGGAGGTTGATATCAGCAGCACCCTAGACAAAGAAACACAGGCAATTTATCTTGAACAAGTTTTGAGAGAAGGCTTTTCGCATCCATCTGTGAATGGGATAATGCTTTGGACTGCAATGCATCCTAATGGGTGCTACCAAATGTGCCTCACGGACAATAATCTCCAAAACCTACCTGCTGGTGATGTGGTGGACAAGCTCTTGAAAGAATGGCAAACTGGGGAGATAGAGGGGCAGACAGATGAGCATGGATCTTACAGCTTCTATGGTTTCTTGGGTGAATATAAAGTAAGTGTCAAATATGGTAATAGATCTTCAAGTTCAACATTATCATTGTGTCAAGGTAAAGAAACTAGACATGTTAGCATTCAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_10

Protein Analysis

474

Amino Acids

53.65

Weight (kDa)

5.75

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 128 - 393 3.9e-47 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 215, 963
AatII GACGTC 1 cut(s) 735
Acc36I ACCTGC 2 cut(s) 669, 1228
AccII CGCG 2 cut(s) 506, 570
AciI CCGC 2 cut(s) 476, 629
AclWI GGATC 2 cut(s) 412, 1303
AcoI YGGCCR 1 cut(s) 1017
AcsI RAATTY 1 cut(s) 883
AcyI GRCGYC 1 cut(s) 732
AfaI GTAC 3 cut(s) 503, 707, 978
AfiI CCNNNNNNNGG 1 cut(s) 521
AflIII ACRYGT 1 cut(s) 1402
AgsI TTSAA 8 cut(s) 227, 263, 469, 909, 1100, 1249, 1362, 1368
AluBI AGCT 6 cut(s) 56, 237, 775, 922, 1243, 1305
AluI AGCT 6 cut(s) 56, 237, 775, 922, 1243, 1305
Alw21I GWGCWC 1 cut(s) 275
Alw26I GTCTC 4 cut(s) 334, 388, 800, 860
AlwI GGATC 2 cut(s) 412, 1303
AoxI GGCC 3 cut(s) 778, 1017, 1027
ApeKI GCWGC 6 cut(s) 247, 352, 473, 556, 654, 1062
ApoI RAATTY 1 cut(s) 883
Asp700I GAANNNNTTC 1 cut(s) 747
AspLEI GCGC 2 cut(s) 570, 572
AspS9I GGNCC 1 cut(s) 779
AsuHPI GGTGA 4 cut(s) 159, 640, 1238, 1334
AsuII TTCGAA 1 cut(s) 640
BalI TGGCCA 1 cut(s) 1019
BarI GAAGNNNNNNTAC 2 cut(s) 1342, 1374
BbsI GAAGAC 1 cut(s) 618
Bbv12I GWGCWC 1 cut(s) 275
BbvI GCAGC 6 cut(s) 259, 339, 485, 543, 666, 1074
BccI CCATC 4 cut(s) 178, 937, 941, 1138
BceAI ACGGC 3 cut(s) 299, 656, 825
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BclI TGATCA 1 cut(s) 291
BcoDI GTCTC 4 cut(s) 334, 388, 800, 860
BfaI CTAG 4 cut(s) 428, 588, 1070, 1398
BfuAI ACCTGC 2 cut(s) 669, 1228
BglII AGATCT 1 cut(s) 1355
BisI GCNGC 7 cut(s) 248, 353, 474, 557, 629, 655, 1063
BlpI GCTNAGC 2 cut(s) 52, 120
BlsI GCNGC 7 cut(s) 249, 354, 475, 558, 630, 656, 1064
BmgT120I GGNCC 1 cut(s) 779
BmiI GGNNCC 2 cut(s) 513, 781
BmrI ACTGGG 1 cut(s) 1272
BmsI GCATC 3 cut(s) 119, 1135, 1174
BmuI ACTGGG 1 cut(s) 1272
BpiI GAAGAC 1 cut(s) 618
BplI GAGNNNNNCTC 2 cut(s) 368, 400
BpmI CTGGAG 2 cut(s) 664, 980
Bpu10I CCTNAGC 1 cut(s) 923
Bpu1102I GCTNAGC 2 cut(s) 52, 120
Bpu14I TTCGAA 1 cut(s) 640
BpuEI CTTGAG 2 cut(s) 128, 479
BsaAI YACGTR 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 732
BsaXI ACNNNNNCTCC 2 cut(s) 969, 999
Bsc4I CCNNNNNNNGG 1 cut(s) 521
Bse118I RCCGGY 1 cut(s) 650
Bse1I ACTGG 2 cut(s) 963, 1267
Bse3DI GCAATG 5 cut(s) 540, 582, 638, 791, 1167
BseGI GGATG 5 cut(s) 134, 170, 952, 1126, 1165
BseLI CCNNNNNNNGG 1 cut(s) 521
BseMI GCAATG 5 cut(s) 540, 582, 638, 791, 1167
BseMII CTCAG 2 cut(s) 66, 345
BseNI ACTGG 2 cut(s) 963, 1267
BsePI GCGCGC 1 cut(s) 568
BseXI GCAGC 6 cut(s) 259, 339, 485, 543, 666, 1074
BsgI GTGCAG 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 506, 570
Bsh1285I CGRYCG 1 cut(s) 891
BshFI GGCC 3 cut(s) 780, 1019, 1029
BsiEI CGRYCG 1 cut(s) 891
BsiHKAI GWGCWC 1 cut(s) 275
BsiSI CCGG 1 cut(s) 651
BslFI GGGAC 1 cut(s) 743
BslI CCNNNNNNNGG 1 cut(s) 521
BsmAI GTCTC 4 cut(s) 334, 388, 800, 860
BsmBI CGTCTC 1 cut(s) 800
BsmFI GGGAC 1 cut(s) 743
BsmI GAATGC 2 cut(s) 205, 1410
BsnI GGCC 3 cut(s) 780, 1019, 1029
Bsp119I TTCGAA 1 cut(s) 640
Bsp1286I GDGCHC 1 cut(s) 275
Bsp143I GATC 5 cut(s) 291, 404, 547, 1295, 1355
Bsp1720I GCTNAGC 2 cut(s) 52, 120
BspACI CCGC 2 cut(s) 476, 629
BspANI GGCC 3 cut(s) 780, 1019, 1029
BspCNI CTCAG 2 cut(s) 65, 344
BspFNI CGCG 2 cut(s) 506, 570
BspHI TCATGA 1 cut(s) 843
BspLI GGNNCC 2 cut(s) 513, 781
BspMI ACCTGC 2 cut(s) 669, 1228
BspPI GGATC 2 cut(s) 412, 1303
BspQI GCTCTTC 1 cut(s) 244
BspT104I TTCGAA 1 cut(s) 640
BsrDI GCAATG 5 cut(s) 540, 582, 638, 791, 1167
BsrFI RCCGGY 1 cut(s) 650
BsrI ACTGG 2 cut(s) 963, 1267
BssAI RCCGGY 1 cut(s) 650
BssHII GCGCGC 1 cut(s) 568
BssMI GATC 5 cut(s) 291, 404, 547, 1295, 1355
BssNI GRCGYC 1 cut(s) 732
Bst4CI ACNGT 4 cut(s) 112, 322, 892, 976
Bst6I CTCTTC 2 cut(s) 244, 708
BstACI GRCGYC 1 cut(s) 732
BstAPI GCANNNNNTGC 1 cut(s) 565
BstBAI YACGTR 1 cut(s) 276
BstBI TTCGAA 1 cut(s) 640
BstC8I GCNNGC 4 cut(s) 49, 70, 312, 570
BstDEI CTNAG 4 cut(s) 52, 120, 331, 923
BstF5I GGATG 5 cut(s) 134, 170, 952, 1126, 1165
BstFNI CGCG 2 cut(s) 506, 570
BstHHI GCGC 2 cut(s) 570, 572
BstKTI GATC 5 cut(s) 294, 407, 550, 1298, 1358
BstMAI GTCTC 4 cut(s) 334, 388, 800, 860
BstMBI GATC 5 cut(s) 291, 404, 547, 1295, 1355
BstMCI CGRYCG 1 cut(s) 891
BstMWI GCNNNNNNNGC 3 cut(s) 53, 565, 699
BstNSI RCATGY 1 cut(s) 1406
BstUI CGCG 2 cut(s) 506, 570
BstV1I GCAGC 6 cut(s) 259, 339, 485, 543, 666, 1074
BstV2I GAAGAC 1 cut(s) 618
BstX2I RGATCY 3 cut(s) 404, 1295, 1355
BstYI RGATCY 3 cut(s) 404, 1295, 1355
BsuRI GGCC 3 cut(s) 780, 1019, 1029
BtsCI GGATG 5 cut(s) 134, 170, 952, 1126, 1165
BtsI GCAGTG 1 cut(s) 880
BtsIMutI CAGTG 1 cut(s) 880
BveI ACCTGC 2 cut(s) 669, 1228
Cac8I GCNNGC 4 cut(s) 49, 70, 312, 570
CciI TCATGA 1 cut(s) 843
CfoI GCGC 2 cut(s) 570, 572
Cfr10I RCCGGY 1 cut(s) 650
Cfr13I GGNCC 1 cut(s) 779
Csp6I GTAC 3 cut(s) 502, 706, 977
CviAII CATG 6 cut(s) 125, 326, 844, 929, 1292, 1403
CviQI GTAC 3 cut(s) 502, 706, 977
DdeI CTNAG 4 cut(s) 52, 120, 331, 923
DpnI GATC 5 cut(s) 293, 406, 549, 1297, 1357
DpnII GATC 5 cut(s) 291, 404, 547, 1295, 1355
DrdI GACNNNNNNGTC 2 cut(s) 215, 963
DseDI GACNNNNNNGTC 2 cut(s) 215, 963
EaeI YGGCCR 1 cut(s) 1017
Eam1104I CTCTTC 2 cut(s) 244, 708
EarI CTCTTC 2 cut(s) 244, 708
Eco147I AGGCCT 1 cut(s) 1029
Eco32I GATATC 1 cut(s) 1057
EcoRI GAATTC 1 cut(s) 883
EcoRV GATATC 1 cut(s) 1057
EcoT22I ATGCAT 1 cut(s) 1167
Esp3I CGTCTC 1 cut(s) 800
FaeI CATG 6 cut(s) 128, 329, 847, 932, 1295, 1406
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 1 cut(s) 743
FatI CATG 6 cut(s) 124, 325, 843, 928, 1291, 1402
FbaI TGATCA 1 cut(s) 291
Fnu4HI GCNGC 7 cut(s) 248, 353, 474, 557, 629, 655, 1063
FokI GGATG 5 cut(s) 141, 157, 959, 1113, 1152
Fsp4HI GCNGC 7 cut(s) 248, 353, 474, 557, 629, 655, 1063
FspBI CTAG 4 cut(s) 428, 588, 1070, 1398
GlaI GCGC 2 cut(s) 569, 571
GluI GCNGC 7 cut(s) 248, 353, 474, 557, 629, 655, 1063
GsuI CTGGAG 2 cut(s) 664, 980
HaeIII GGCC 3 cut(s) 780, 1019, 1029
HapII CCGG 1 cut(s) 651
HhaI GCGC 2 cut(s) 570, 572
Hin1I GRCGYC 1 cut(s) 732
Hin1II CATG 6 cut(s) 128, 329, 847, 932, 1295, 1406
Hin6I GCGC 2 cut(s) 568, 570
HinP1I GCGC 2 cut(s) 568, 570
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 773
HinfI GANTC 3 cut(s) 465, 684, 896
HpaII CCGG 1 cut(s) 651
HphI GGTGA 4 cut(s) 159, 640, 1238, 1334
Hpy166II GTNNAC 3 cut(s) 220, 676, 1237
Hpy188I TCNGA 4 cut(s) 198, 334, 823, 1416
Hpy188III TCNNGA 6 cut(s) 34, 446, 681, 844, 1097, 1246
Hpy8I GTNNAC 3 cut(s) 220, 676, 1237
Hpy99I CGWCG 1 cut(s) 511
HpyAV CCTTC 2 cut(s) 1040, 1109
HpyCH4III ACNGT 4 cut(s) 112, 322, 892, 976
HpyCH4IV ACGT 2 cut(s) 275, 732
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 565, 699
HpyF3I CTNAG 4 cut(s) 52, 120, 331, 923
HpySE526I ACGT 2 cut(s) 275, 732
Hsp92I GRCGYC 1 cut(s) 732
Hsp92II CATG 6 cut(s) 128, 329, 847, 932, 1295, 1406
HspAI GCGC 2 cut(s) 568, 570
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 5 cut(s) 291, 404, 547, 1295, 1355
LguI GCTCTTC 1 cut(s) 244
LmnI GCTCC 3 cut(s) 270, 511, 935
Lsp1109I GCAGC 6 cut(s) 259, 339, 485, 543, 666, 1074
LweI GCATC 3 cut(s) 119, 1135, 1174
MaeI CTAG 4 cut(s) 428, 588, 1070, 1398
MaeII ACGT 2 cut(s) 275, 732
MaeIII GTNAC 2 cut(s) 316, 593
MalI GATC 5 cut(s) 293, 406, 549, 1297, 1357
MboI GATC 5 cut(s) 291, 404, 547, 1295, 1355
MboII GAAGA 4 cut(s) 231, 623, 725, 1350
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 3 cut(s) 404, 1295, 1355
MhlI GDGCHC 1 cut(s) 275
MlsI TGGCCA 1 cut(s) 1019
MluCI AATT 8 cut(s) 176, 228, 366, 411, 433, 560, 883, 1089
MluNI TGGCCA 1 cut(s) 1019
Mox20I TGGCCA 1 cut(s) 1019
Mph1103I ATGCAT 1 cut(s) 1167
MroXI GAANNNNTTC 1 cut(s) 747
MscI TGGCCA 1 cut(s) 1019
MseI TTAA 1 cut(s) 855
MslI CAYNNNNRTG 2 cut(s) 1170, 1415
Msp20I TGGCCA 1 cut(s) 1019
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 1 cut(s) 651
MunI CAATTG 1 cut(s) 411
Mva1269I GAATGC 2 cut(s) 205, 1410
MvnI CGCG 2 cut(s) 506, 570
MwoI GCNNNNNNNGC 3 cut(s) 53, 565, 699
NdeII GATC 5 cut(s) 291, 404, 547, 1295, 1355
NlaIII CATG 6 cut(s) 128, 329, 847, 932, 1295, 1406
NlaIV GGNNCC 2 cut(s) 513, 781
NmuCI GTSAC 2 cut(s) 316, 593
NsiI ATGCAT 1 cut(s) 1167
NspI RCATGY 1 cut(s) 1406
NspV TTCGAA 1 cut(s) 640
PagI TCATGA 1 cut(s) 843
PauI GCGCGC 1 cut(s) 568
PceI AGGCCT 1 cut(s) 1029
PciI ACATGT 1 cut(s) 1402
PciSI GCTCTTC 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 738
PctI GAATGC 2 cut(s) 205, 1410
PdmI GAANNNNTTC 1 cut(s) 747
PfeI GAWTC 3 cut(s) 465, 684, 896
PkrI GCNGC 7 cut(s) 249, 354, 475, 558, 630, 656, 1064
Ppu21I YACGTR 1 cut(s) 276
PscI ACATGT 1 cut(s) 1402
PspN4I GGNNCC 2 cut(s) 513, 781
PspPI GGNCC 1 cut(s) 779
PsuI RGATCY 3 cut(s) 404, 1295, 1355
PteI GCGCGC 1 cut(s) 568
RsaI GTAC 3 cut(s) 503, 707, 978
RsaNI GTAC 3 cut(s) 502, 706, 977
RseI CAYNNNNRTG 2 cut(s) 1170, 1415
SapI GCTCTTC 1 cut(s) 244
SaqAI TTAA 1 cut(s) 855
SatI GCNGC 7 cut(s) 248, 353, 474, 557, 629, 655, 1063
Sau3AI GATC 5 cut(s) 291, 404, 547, 1295, 1355
Sau96I GGNCC 1 cut(s) 779
SduI GDGCHC 1 cut(s) 275
SfaNI GCATC 3 cut(s) 119, 1135, 1174
SfuI TTCGAA 1 cut(s) 640
SmiMI CAYNNNNRTG 2 cut(s) 1170, 1415
SmlI CTYRAG 2 cut(s) 143, 494
SmoI CTYRAG 2 cut(s) 143, 494
Sse9I AATT 8 cut(s) 176, 228, 366, 411, 433, 560, 883, 1089
SseBI AGGCCT 1 cut(s) 1029
SsiI CCGC 2 cut(s) 476, 629
SspMI CTAG 4 cut(s) 428, 588, 1070, 1398
StuI AGGCCT 1 cut(s) 1029
TaaI ACNGT 4 cut(s) 112, 322, 892, 976
TaiI ACGT 2 cut(s) 278, 735
TaqI TCGA 2 cut(s) 640, 887
TasI AATT 8 cut(s) 176, 228, 366, 411, 433, 560, 883, 1089
TatI WGTACW 1 cut(s) 705
TauI GCSGC 1 cut(s) 631
TfiI GAWTC 3 cut(s) 465, 684, 896
Tru1I TTAA 1 cut(s) 855
Tru9I TTAA 1 cut(s) 855
TscAI CASTG 1 cut(s) 880
TseFI GTSAC 2 cut(s) 316, 593
TseI GCWGC 6 cut(s) 247, 352, 473, 556, 654, 1062
Tsp45I GTSAC 2 cut(s) 316, 593
TspDTI ATGAA 4 cut(s) 102, 314, 832, 860
TspGWI ACGGA 4 cut(s) 319, 524, 1061, 1211
TspRI CASTG 1 cut(s) 880
XapI RAATTY 1 cut(s) 883
XceI RCATGY 1 cut(s) 1406
XmnI GAANNNNTTC 1 cut(s) 747
XspI CTAG 4 cut(s) 428, 588, 1070, 1398
ZraI GACGTC 1 cut(s) 733
Zsp2I ATGCAT 1 cut(s) 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.