Rroxscaffold_7G00216050

Endo-1,4-beta-xylanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004763
Physical Location & Seq
Reverse (-)
66748910 .. 66751926
3017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00216050.1

Sequence Viewer

Length: 1653 bp
ATGGGAAACGCTACTCTCACCTTCGTCAGGCTGCAGCTTCTTATGTTCTTTCTCCTCATGGGCTCACCCTTGGTTTTTTCCTTTGATGGTCCACTATATGACTCTACTGCTTACACTGAGTGTAAAAGGCAGCCAGAGAAGCCTCTATATGGTGGAGGGATCATTGTTGATGAGCCTTCAGCCGTTGTAGACATAGACAATGGGGACGTTTATTCACCGGCATTCCTACTCCAAAATCTCACCGGCAACGGCACCATTTACAGCTTCTCCAGTTGGAAAAAGAACGTTGTTAGATACAGGTGGGAGGGTTGGGTGACGATAGGGGGTGCAGGTTCAGCACTGATACAGGCAAGTCTAAAGGCAGAAGATGAATCATACAATTGTATTGGAACTGTTTTGGCCAAGCGGGGATGCTGGTCATTCCTCAAGGGTGGCTTTGTCCTAAAGTCACCACCTAATTTCTCTATACTATTCTTCCAGAATACGGATGGCAGAGATGTCGGCCTAGCAATTGCAAGTGCTTCTTTGCAGCCATTTAGTGTTCAGCAATGGAATACATATCACCAATATATGATCAACACTAAAAGGAAGCGTGCCGTGACGGTCCATGTCTCAAATGGACAAGGAAAGGGGCTTCAAGGAGCTGCAATTACCGTAGAGCAAGTCTCCATAGATTTCCCATTTGGATCTGCAATTTCAAAGACCATTCTAGGCAATTTACCCTACCAGTTACAATACTTTATGATTGATCTTCACATTCAGAATTGGTTTGTTGAGAGATTCAATGCTGCGGTGTTCGAGAATGAACTCAAGTGGTATGCAACAGAACCTAATCAAGGCAATGTCACTTACACTATAGCAGATCAAATGTTGCAATTTGTTCGAGCTCACCAAATCACTGCTAGAGGGCACAATATATTCTGGGAAGATCCCAAATACACACCACCTTGGGTTCGATACCTCACAGCTGATCAGCTAAAATCAGCTGTCAACTCTCGGATACAAAGCCTAATGAGCAAATACAAAGAAGAGTTCATTCATTGGGATGTCAGCAATGAAATGCTTCACTTTGATTTCTATGAGAAACAACTTGGCCCCAATGCCACCTTGGATTTCTTTCAGACGGCACACAAATCTGATCCTTTGGCAACCCTTTTTATGAATGATTTTAATGTGGTGGAAACTTGCACTGATGTGAATTCCACAGTTGATACCTATGTTTCAAGGCTGAGAGAACTTCAACAAGGTGGAGTATCAATGGATGGGATTGGACTAGAGGAGGTTGATATCAGCAATACTCTAGACAAACAAACACAGGCCATTTATCTAGAGCAAGTATTAAGAGAAGGTTTCTCACATCCATCTGTAAATGGGATAATGCTCTGGAGTGCTCTACATCCTAACGGGTGCTACGAGATGTGCCTGACGGATAATAACCTTAAAAATCTACCAGCTGGTGATGTGGTGGACAAGCTCTTGAAAGAATGGCAAACTGGGAAGATAGAGGCTCAGATCGATGAGCATGGAACACATAGCTTTTTCGGGTTCTTGGGTGAGTACAGGGTTAGTGTGAAGTATGGAAATAGGACCGCAAACTCAACATTCTCACTATGTGAAGGGGAAGAAACTAGACATGTTAGCATTCAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_10

Protein Analysis

550

Amino Acids

62.02

Weight (kDa)

5.44

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 252 - 427 1e-32 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 189
AciI CCGC 3 cut(s) 406, 791, 1590
AclI AACGTT 1 cut(s) 285
AclWI GGATC 4 cut(s) 167, 694, 923, 1133
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 1 cut(s) 1198
AcuI CTGAAG 1 cut(s) 162
AdeI CACNNNGTG 2 cut(s) 120, 1613
AfaI GTAC 1 cut(s) 1559
AfiI CCNNNNNNNGG 4 cut(s) 27, 149, 484, 836
AflIII ACRYGT 1 cut(s) 1633
AgsI TTSAA 7 cut(s) 638, 699, 784, 1224, 1241, 1480, 1646
AleI CACNNNNGTG 1 cut(s) 1193
Alw21I GWGCWC 2 cut(s) 889, 1393
Alw26I GTCTC 2 cut(s) 616, 670
AlwI GGATC 4 cut(s) 167, 694, 923, 1133
AoxI GGCC 4 cut(s) 399, 502, 1093, 1317
ApeKI GCWGC 6 cut(s) 31, 34, 130, 529, 644, 788
ApoI RAATTY 1 cut(s) 1198
Asp700I GAANNNNTTC 1 cut(s) 1062
AspS9I GGNCC 4 cut(s) 89, 604, 1094, 1587
AvaII GGWCC 3 cut(s) 89, 604, 1587
BaeGI GKGCMC 1 cut(s) 912
BaeI ACNNNNGTAYC 2 cut(s) 1203, 1236
BalI TGGCCA 1 cut(s) 401
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 2 cut(s) 65, 889
Bbv12I GWGCWC 2 cut(s) 889, 1393
BbvI GCAGC 6 cut(s) 18, 46, 142, 541, 631, 775
BccI CCATC 4 cut(s) 80, 482, 1256, 1369
BceAI ACGGC 4 cut(s) 167, 265, 581, 1140
BcgI CGANNNNNNTGC 4 cut(s) 481, 515, 863, 897
BciVI GTATCC 1 cut(s) 993
BclI TGATCA 2 cut(s) 573, 970
BcoDI GTCTC 2 cut(s) 616, 670
BfaI CTAG 7 cut(s) 506, 710, 903, 1274, 1301, 1328, 1629
BfmI CTRYAG 2 cut(s) 32, 855
BfuAI ACCTGC 1 cut(s) 320
BfuI GTATCC 1 cut(s) 993
BisI GCNGC 6 cut(s) 32, 35, 131, 530, 645, 789
BlsI GCNGC 6 cut(s) 33, 36, 132, 531, 646, 790
Bme18I GGWCC 3 cut(s) 89, 604, 1587
BmgT120I GGNCC 4 cut(s) 89, 604, 1094, 1587
BmiI GGNNCC 2 cut(s) 253, 1096
BmrI ACTGGG 1 cut(s) 1503
BmsI GCATC 1 cut(s) 401
BmuI ACTGGG 1 cut(s) 1503
BplI GAGNNNNNCTC 2 cut(s) 650, 682
BpmI CTGGAG 2 cut(s) 253, 1405
BpuEI CTTGAG 2 cut(s) 410, 794
Bsa29I ATCGAT 1 cut(s) 1515
BsaJI CCNNGG 3 cut(s) 69, 947, 1107
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 149, 484, 836
Bse118I RCCGGY 2 cut(s) 217, 242
Bse1I ACTGG 3 cut(s) 270, 727, 1498
Bse3DI GCAATG 3 cut(s) 554, 847, 1060
BseCI ATCGAT 1 cut(s) 1515
BseDI CCNNGG 3 cut(s) 69, 947, 1107
BseGI GGATG 6 cut(s) 416, 493, 1051, 1267, 1357, 1396
BseLI CCNNNNNNNGG 4 cut(s) 27, 149, 484, 836
BseMI GCAATG 3 cut(s) 554, 847, 1060
BseMII CTCAG 3 cut(s) 108, 1220, 1523
BseNI ACTGG 3 cut(s) 270, 727, 1498
BseRI GAGGAG 2 cut(s) 44, 1292
BseSI GKGCMC 1 cut(s) 912
BseXI GCAGC 6 cut(s) 18, 46, 142, 541, 631, 775
BsgI GTGCAG 1 cut(s) 348
BshFI GGCC 4 cut(s) 401, 504, 1095, 1319
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 1515
BsiHKAI GWGCWC 2 cut(s) 889, 1393
BsiSI CCGG 2 cut(s) 218, 243
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 4 cut(s) 27, 149, 484, 836
BsmAI GTCTC 2 cut(s) 616, 670
BsmFI GGGAC 1 cut(s) 218
BsmI GAATGC 2 cut(s) 221, 1641
BsnI GGCC 4 cut(s) 401, 504, 1095, 1319
Bsp1286I GDGCHC 4 cut(s) 65, 889, 912, 1393
Bsp143I GATC 9 cut(s) 159, 573, 686, 748, 862, 928, 970, 1138, 1512
BspACI CCGC 3 cut(s) 406, 791, 1590
BspANI GGCC 4 cut(s) 401, 504, 1095, 1319
BspCNI CTCAG 3 cut(s) 109, 1221, 1522
BspDI ATCGAT 1 cut(s) 1515
BspLI GGNNCC 2 cut(s) 253, 1096
BspMAI CTGCAG 1 cut(s) 36
BspMI ACCTGC 1 cut(s) 320
BspPI GGATC 4 cut(s) 167, 694, 923, 1133
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 3 cut(s) 554, 847, 1060
BsrFI RCCGGY 2 cut(s) 217, 242
BsrI ACTGG 3 cut(s) 270, 727, 1498
BssAI RCCGGY 2 cut(s) 217, 242
BssECI CCNNGG 3 cut(s) 69, 947, 1107
BssMI GATC 9 cut(s) 159, 573, 686, 748, 862, 928, 970, 1138, 1512
BssT1I CCWWGG 3 cut(s) 69, 947, 1107
Bst4CI ACNGT 5 cut(s) 394, 604, 655, 1207, 1650
Bst6I CTCTTC 1 cut(s) 1023
BstC8I GCNNGC 1 cut(s) 594
BstDEI CTNAG 3 cut(s) 117, 1229, 1509
BstENI CCTNNNNNAGG 2 cut(s) 25, 834
BstF5I GGATG 6 cut(s) 416, 493, 1051, 1267, 1357, 1396
BstKTI GATC 9 cut(s) 162, 576, 689, 751, 865, 931, 973, 1141, 1515
BstMAI GTCTC 2 cut(s) 616, 670
BstMBI GATC 9 cut(s) 159, 573, 686, 748, 862, 928, 970, 1138, 1512
BstMWI GCNNNNNNNGC 3 cut(s) 139, 335, 1014
BstNSI RCATGY 1 cut(s) 1637
BstSFI CTRYAG 2 cut(s) 32, 855
BstSLI GKGCMC 1 cut(s) 912
BstV1I GCAGC 6 cut(s) 18, 46, 142, 541, 631, 775
BstX2I RGATCY 2 cut(s) 686, 928
BstYI RGATCY 2 cut(s) 686, 928
Bsu15I ATCGAT 1 cut(s) 1515
BsuI GTATCC 1 cut(s) 993
BsuRI GGCC 4 cut(s) 401, 504, 1095, 1319
BsuTUI ATCGAT 1 cut(s) 1515
BtsCI GGATG 6 cut(s) 416, 493, 1051, 1267, 1357, 1396
BtsI GCAGTG 1 cut(s) 897
BtsIMutI CAGTG 4 cut(s) 114, 338, 897, 1188
BveI ACCTGC 1 cut(s) 320
Cac8I GCNNGC 1 cut(s) 594
Cfr10I RCCGGY 2 cut(s) 217, 242
Cfr13I GGNCC 4 cut(s) 89, 604, 1094, 1587
ClaI ATCGAT 1 cut(s) 1515
Csp6I GTAC 1 cut(s) 1558
CviAII CATG 4 cut(s) 58, 608, 1523, 1634
CviQI GTAC 1 cut(s) 1558
DdeI CTNAG 3 cut(s) 117, 1229, 1509
DpnI GATC 9 cut(s) 161, 575, 688, 750, 864, 930, 972, 1140, 1514
DpnII GATC 9 cut(s) 159, 573, 686, 748, 862, 928, 970, 1138, 1512
DraIII CACNNNGTG 2 cut(s) 120, 1613
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 1023
Ecl136II GAGCTC 1 cut(s) 887
Eco130I CCWWGG 3 cut(s) 69, 947, 1107
Eco24I GRGCYC 2 cut(s) 65, 889
Eco32I GATATC 1 cut(s) 1288
Eco47I GGWCC 3 cut(s) 89, 604, 1587
Eco53kI GAGCTC 1 cut(s) 887
Eco57I CTGAAG 1 cut(s) 162
EcoICRI GAGCTC 1 cut(s) 887
EcoNI CCTNNNNNAGG 2 cut(s) 25, 834
EcoRI GAATTC 1 cut(s) 1198
EcoRV GATATC 1 cut(s) 1288
EcoT14I CCWWGG 3 cut(s) 69, 947, 1107
EcoT38I GRGCYC 2 cut(s) 65, 889
ErhI CCWWGG 3 cut(s) 69, 947, 1107
FaeI CATG 4 cut(s) 61, 611, 1526, 1637
FalI AAGNNNNNCTT 4 cut(s) 419, 451, 508, 540
FaqI GGGAC 1 cut(s) 218
FatI CATG 4 cut(s) 57, 607, 1522, 1633
FauI CCCGC 1 cut(s) 399
FbaI TGATCA 2 cut(s) 573, 970
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 6 cut(s) 32, 35, 131, 530, 645, 789
FokI GGATG 6 cut(s) 423, 500, 1058, 1274, 1344, 1383
FriOI GRGCYC 2 cut(s) 65, 889
Fsp4HI GCNGC 6 cut(s) 32, 35, 131, 530, 645, 789
FspBI CTAG 7 cut(s) 506, 710, 903, 1274, 1301, 1328, 1629
GluI GCNGC 6 cut(s) 32, 35, 131, 530, 645, 789
GsuI CTGGAG 2 cut(s) 253, 1405
HaeIII GGCC 4 cut(s) 401, 504, 1095, 1319
HapII CCGG 2 cut(s) 218, 243
Hin1II CATG 4 cut(s) 61, 611, 1526, 1637
HincII GTYRAC 1 cut(s) 991
HindII GTYRAC 1 cut(s) 991
HinfI GANTC 3 cut(s) 101, 371, 780
HpaII CCGG 2 cut(s) 218, 243
Hpy166II GTNNAC 4 cut(s) 92, 190, 991, 1468
Hpy188I TCNGA 5 cut(s) 762, 999, 1122, 1138, 1512
Hpy188III TCNNGA 6 cut(s) 478, 799, 1301, 1328, 1384, 1477
Hpy8I GTNNAC 4 cut(s) 92, 190, 991, 1468
HpyAV CCTTC 4 cut(s) 31, 186, 1340, 1610
HpyCH4III ACNGT 5 cut(s) 394, 604, 655, 1207, 1650
HpyCH4IV ACGT 2 cut(s) 207, 285
HpyCH4V TGCA 9 cut(s) 34, 329, 515, 529, 647, 692, 821, 874, 1188
HpyF10VI GCNNNNNNNGC 3 cut(s) 139, 335, 1014
HpyF3I CTNAG 3 cut(s) 117, 1229, 1509
HpySE526I ACGT 2 cut(s) 207, 285
Hsp92II CATG 4 cut(s) 61, 611, 1526, 1637
Ksp22I TGATCA 2 cut(s) 573, 970
Kzo9I GATC 9 cut(s) 159, 573, 686, 748, 862, 928, 970, 1138, 1512
LmnI GCTCC 1 cut(s) 641
Lsp1109I GCAGC 6 cut(s) 18, 46, 142, 541, 631, 775
LweI GCATC 1 cut(s) 401
MaeI CTAG 7 cut(s) 506, 710, 903, 1274, 1301, 1328, 1629
MaeII ACGT 2 cut(s) 207, 285
MaeIII GTNAC 5 cut(s) 313, 447, 598, 729, 844
MalI GATC 9 cut(s) 161, 575, 688, 750, 864, 930, 972, 1140, 1514
MboI GATC 9 cut(s) 159, 573, 686, 748, 862, 928, 970, 1138, 1512
MboII GAAGA 7 cut(s) 377, 466, 743, 938, 1040, 1510, 1634
MfeI CAATTG 2 cut(s) 379, 510
MflI RGATCY 2 cut(s) 686, 928
MhlI GDGCHC 4 cut(s) 65, 889, 912, 1393
MlsI TGGCCA 1 cut(s) 401
MluCI AATT 9 cut(s) 379, 457, 510, 648, 693, 715, 763, 875, 1198
MluNI TGGCCA 1 cut(s) 401
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 1 cut(s) 254
Mox20I TGGCCA 1 cut(s) 401
MroXI GAANNNNTTC 1 cut(s) 1062
MscI TGGCCA 1 cut(s) 401
MseI TTAA 3 cut(s) 1170, 1340, 1440
MslI CAYNNNNRTG 2 cut(s) 1044, 1193
Msp20I TGGCCA 1 cut(s) 401
MspA1I CMGCKG 3 cut(s) 968, 986, 1454
MspI CCGG 2 cut(s) 218, 243
MunI CAATTG 2 cut(s) 379, 510
Mva1269I GAATGC 2 cut(s) 221, 1641
MwoI GCNNNNNNNGC 3 cut(s) 139, 335, 1014
NdeII GATC 9 cut(s) 159, 573, 686, 748, 862, 928, 970, 1138, 1512
NlaIII CATG 4 cut(s) 61, 611, 1526, 1637
NlaIV GGNNCC 2 cut(s) 253, 1096
NmuCI GTSAC 4 cut(s) 313, 447, 598, 844
NspI RCATGY 1 cut(s) 1637
OliI CACNNNNGTG 1 cut(s) 1193
PciI ACATGT 1 cut(s) 1633
PcsI WCGNNNNNNNCGW 1 cut(s) 1410
PctI GAATGC 2 cut(s) 221, 1641
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 2 cut(s) 371, 780
PkrI GCNGC 6 cut(s) 33, 36, 132, 531, 646, 790
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PscI ACATGT 1 cut(s) 1633
Psp124BI GAGCTC 1 cut(s) 889
Psp1406I AACGTT 1 cut(s) 285
PspN4I GGNNCC 2 cut(s) 253, 1096
PspPI GGNCC 4 cut(s) 89, 604, 1094, 1587
PstI CTGCAG 1 cut(s) 36
PsuI RGATCY 2 cut(s) 686, 928
PvuII CAGCTG 3 cut(s) 968, 986, 1454
RsaI GTAC 1 cut(s) 1559
RsaNI GTAC 1 cut(s) 1558
RseI CAYNNNNRTG 2 cut(s) 1044, 1193
SacI GAGCTC 1 cut(s) 889
SaqAI TTAA 3 cut(s) 1170, 1340, 1440
SatI GCNGC 6 cut(s) 32, 35, 131, 530, 645, 789
Sau3AI GATC 9 cut(s) 159, 573, 686, 748, 862, 928, 970, 1138, 1512
Sau96I GGNCC 4 cut(s) 89, 604, 1094, 1587
SchI GAGTC 1 cut(s) 95
SduI GDGCHC 4 cut(s) 65, 889, 912, 1393
SfaNI GCATC 1 cut(s) 401
SfcI CTRYAG 2 cut(s) 32, 855
SinI GGWCC 3 cut(s) 89, 604, 1587
SmiMI CAYNNNNRTG 2 cut(s) 1044, 1193
SmlI CTYRAG 2 cut(s) 425, 809
SmoI CTYRAG 2 cut(s) 425, 809
Sse9I AATT 9 cut(s) 379, 457, 510, 648, 693, 715, 763, 875, 1198
SsiI CCGC 3 cut(s) 406, 791, 1590
SspMI CTAG 7 cut(s) 506, 710, 903, 1274, 1301, 1328, 1629
SstI GAGCTC 1 cut(s) 889
StyI CCWWGG 3 cut(s) 69, 947, 1107
TaaI ACNGT 5 cut(s) 394, 604, 655, 1207, 1650
TaiI ACGT 2 cut(s) 210, 288
TaqI TCGA 4 cut(s) 798, 883, 955, 1515
TasI AATT 9 cut(s) 379, 457, 510, 648, 693, 715, 763, 875, 1198
TatI WGTACW 1 cut(s) 1557
TfiI GAWTC 2 cut(s) 371, 780
Tru1I TTAA 3 cut(s) 1170, 1340, 1440
Tru9I TTAA 3 cut(s) 1170, 1340, 1440
TscAI CASTG 4 cut(s) 121, 345, 904, 1195
TseFI GTSAC 4 cut(s) 313, 447, 598, 844
TseI GCWGC 6 cut(s) 31, 34, 130, 529, 644, 788
Tsp45I GTSAC 4 cut(s) 313, 447, 598, 844
TspDTI ATGAA 6 cut(s) 384, 819, 1024, 1028, 1071, 1175
TspGWI ACGGA 2 cut(s) 500, 1442
TspRI CASTG 4 cut(s) 121, 345, 904, 1195
VpaK11BI GGWCC 3 cut(s) 89, 604, 1587
XagI CCTNNNNNAGG 2 cut(s) 25, 834
XapI RAATTY 1 cut(s) 1198
XbaI TCTAGA 2 cut(s) 1300, 1327
XceI RCATGY 1 cut(s) 1637
XcmI CCANNNNNNNNNTGG 3 cut(s) 485, 614, 1105
XmiI GTMKAC 1 cut(s) 189
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 7 cut(s) 506, 710, 903, 1274, 1301, 1328, 1629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.