MD05G1119000.v1.1

Endo-1,4-beta-xylanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004763
Physical Location & Seq
Reverse (-)
24022103 .. 24025546
3444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1119000.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGCTGGTCATTTCTCAAGGGTGGCTTTGTCCTAACCTCGCCATCCCAATTATCCATCTTATTCTTTCAGAAATTGCCAGCTCTTCTTTGCAGCCATTTAGTGTTCAACAATGGAATGCATATCAGGAATATATGATCAACACCAAACGGAAGCGTGCCGTGACGGTTCATGTCTCAGATATGCAGGGAAAGAGGCTGCAAGGAGCGGCAATTGACGTAGAGCAAGTCTCCAAGGATTTCCCATTTGGATCTGCAATTGCAAAGACCATTCTAGGCAATCTGCCCTATCAGAATTGGTTCGCAAAACGATTCAATGCCGCGGTGTTTGAGAACGAACTCAAGTGGTACGCAACGGAACCTGAACAGGGCAAAACCAACTACACCGTTCCAGATCAAATGCTGCAATTTGTGCGTGCCAACCGAGTCATTGCTAGAGGTCACAACATATTCTGGGAAGATCCCAAATACACGCCGCCATGGGTTCGAAACCTCACCGGCAGCGAGCTGCATTTGGCGGTAAACTCCCGAATCCAAAGCCTAATGAGCAAGTACAGAGAAGAGTTCGTCCATTGGGACGTCAGCAATGAAATGCTTCACTTTGATTTCTATGAGCAGAAGCTTGGCCCCAATGCCACATTGCATTTCTTCGAGACGGCACACAAATCTGACCCTTTGGCAACCCTTTTCATGAATGATTTTAATGTGCTGGAGACTTGCAGCGATGTAAATTCGACCGTTGATTCCTACATTTCAAGGTTGAGGGAGCTTAGGCATGGTGGAGCATTGATGGATGGGATTGGACTAGAGGGTCATTTTACAGTACCAAACCTCCCTCTAATGAGAGCAATCCTTGACAAGTTGGCCACATTAGGCCTTCCCATTTGGCTCACAGAGGTTGATATCAGCAGCACTCTAGACAAAGAAACACAGGCAATTTATCTAGAACAAGTTTTACGAGAAGGCTTCTCACATCCATTGGTGAATGGGATAATGCTTTGGACTGCATTGCATCCTAATGGGTGCTACCAAATGTGCCTAACAGACGATAACCTTCAAAACCTACCTGCTGGTGATGTGGTGGACAAACTCTTGAAAGAATGGCAAACTGGGGAGGTAGAGGGCCAGACAGATGAGCATGGATCATACAGCTTCTATGGTTTCTTGGGTGAATATAGAGTAAGTATCAAGTATGGCAATAGGACTTCAAGCTCAACATTCTCATTGTGCCAAGGTGAAGAAACCAGACATGTTAGCATTCAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_10

Protein Analysis

422

Amino Acids

47.95

Weight (kDa)

5.54

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 76 - 336 1.7e-43 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 807
AatII GACGTC 1 cut(s) 579
Acc36I ACCTGC 1 cut(s) 1072
AccBSI CCGCTC 1 cut(s) 206
AccII CGCG 1 cut(s) 320
AciI CCGC 5 cut(s) 206, 318, 320, 473, 515
AclWI GGATC 3 cut(s) 256, 452, 1147
AcoI YGGCCR 1 cut(s) 861
AcsI RAATTY 1 cut(s) 727
AcyI GRCGYC 1 cut(s) 576
AfaI GTAC 3 cut(s) 347, 551, 822
AfiI CCNNNNNNNGG 1 cut(s) 365
AflIII ACRYGT 1 cut(s) 1246
AgsI TTSAA 7 cut(s) 107, 313, 753, 1055, 1093, 1206, 1259
AluBI AGCT 6 cut(s) 81, 505, 619, 766, 1149, 1209
AluI AGCT 6 cut(s) 81, 505, 619, 766, 1149, 1209
Alw26I GTCTC 4 cut(s) 178, 232, 644, 704
AlwI GGATC 3 cut(s) 256, 452, 1147
AoxI GGCC 4 cut(s) 622, 861, 871, 1120
ApeKI GCWGC 7 cut(s) 91, 196, 400, 498, 505, 717, 906
ApoI RAATTY 1 cut(s) 727
Asp700I GAANNNNTTC 2 cut(s) 296, 591
AspS9I GGNCC 2 cut(s) 623, 1120
AsuHPI GGTGA 5 cut(s) 484, 991, 1082, 1178, 1244
AsuII TTCGAA 1 cut(s) 484
BalI TGGCCA 1 cut(s) 863
BbvI GCAGC 7 cut(s) 103, 183, 387, 492, 510, 729, 918
BccI CCATC 4 cut(s) 50, 63, 781, 785
BceAI ACGGC 2 cut(s) 143, 669
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 4 cut(s) 178, 232, 644, 704
BfaI CTAG 5 cut(s) 272, 432, 803, 914, 941
BfuAI ACCTGC 1 cut(s) 1072
BmgT120I GGNCC 2 cut(s) 623, 1120
BmiI GGNNCC 2 cut(s) 357, 625
BmrI ACTGGG 1 cut(s) 1116
BmsI GCATC 1 cut(s) 1018
BmuI ACTGGG 1 cut(s) 1116
BplI GAGNNNNNCTC 2 cut(s) 212, 244
BpmI CTGGAG 1 cut(s) 728
Bpu10I CCTNAGC 1 cut(s) 767
Bpu14I TTCGAA 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 323
BsaHI GRCGYC 1 cut(s) 576
BsaJI CCNNGG 4 cut(s) 231, 318, 476, 1228
BsaXI ACNNNNNCTCC 2 cut(s) 813, 843
Bsc4I CCNNNNNNNGG 1 cut(s) 365
Bse118I RCCGGY 1 cut(s) 494
Bse1I ACTGG 1 cut(s) 1111
Bse3DI GCAATG 4 cut(s) 426, 589, 635, 1004
BseDI CCNNGG 4 cut(s) 231, 318, 476, 1228
BseGI GGATG 4 cut(s) 42, 796, 970, 1009
BseLI CCNNNNNNNGG 1 cut(s) 365
BseMI GCAATG 4 cut(s) 426, 589, 635, 1004
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 1 cut(s) 1111
BseXI GCAGC 7 cut(s) 103, 183, 387, 492, 510, 729, 918
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 735
BshFI GGCC 4 cut(s) 624, 863, 873, 1122
BsiEI CGRYCG 1 cut(s) 735
BsiSI CCGG 1 cut(s) 495
BslFI GGGAC 1 cut(s) 587
BslI CCNNNNNNNGG 1 cut(s) 365
BsmAI GTCTC 4 cut(s) 178, 232, 644, 704
BsmBI CGTCTC 1 cut(s) 644
BsmFI GGGAC 1 cut(s) 587
BsmI GAATGC 2 cut(s) 121, 1254
BsnI GGCC 4 cut(s) 624, 863, 873, 1122
Bsp119I TTCGAA 1 cut(s) 484
Bsp143I GATC 5 cut(s) 135, 248, 391, 457, 1139
Bsp19I CCATGG 1 cut(s) 476
BspACI CCGC 5 cut(s) 206, 318, 320, 473, 515
BspANI GGCC 4 cut(s) 624, 863, 873, 1122
BspCNI CTCAG 1 cut(s) 188
BspFNI CGCG 1 cut(s) 320
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 2 cut(s) 357, 625
BspMI ACCTGC 1 cut(s) 1072
BspPI GGATC 3 cut(s) 256, 452, 1147
BspQI GCTCTTC 1 cut(s) 88
BspT104I TTCGAA 1 cut(s) 484
BsrBI CCGCTC 1 cut(s) 206
BsrDI GCAATG 4 cut(s) 426, 589, 635, 1004
BsrFI RCCGGY 1 cut(s) 494
BsrI ACTGG 1 cut(s) 1111
BssAI RCCGGY 1 cut(s) 494
BssECI CCNNGG 4 cut(s) 231, 318, 476, 1228
BssMI GATC 5 cut(s) 135, 248, 391, 457, 1139
BssNI GRCGYC 1 cut(s) 576
BssT1I CCWWGG 3 cut(s) 231, 476, 1228
Bst4CI ACNGT 4 cut(s) 166, 385, 736, 820
Bst6I CTCTTC 2 cut(s) 88, 552
BstACI GRCGYC 1 cut(s) 576
BstAPI GCANNNNNTGC 1 cut(s) 409
BstBI TTCGAA 1 cut(s) 484
BstC8I GCNNGC 4 cut(s) 79, 156, 414, 503
BstDEI CTNAG 2 cut(s) 175, 767
BstDSI CCRYGG 2 cut(s) 318, 476
BstF5I GGATG 4 cut(s) 42, 796, 970, 1009
BstFNI CGCG 1 cut(s) 320
BstKTI GATC 5 cut(s) 138, 251, 394, 460, 1142
BstMAI GTCTC 4 cut(s) 178, 232, 644, 704
BstMBI GATC 5 cut(s) 135, 248, 391, 457, 1139
BstMCI CGRYCG 1 cut(s) 735
BstMWI GCNNNNNNNGC 2 cut(s) 409, 543
BstNSI RCATGY 1 cut(s) 1250
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 7 cut(s) 103, 183, 387, 492, 510, 729, 918
BstX2I RGATCY 2 cut(s) 248, 457
BstYI RGATCY 2 cut(s) 248, 457
BsuRI GGCC 4 cut(s) 624, 863, 873, 1122
BtgI CCRYGG 2 cut(s) 318, 476
BtgZI GCGATG 1 cut(s) 735
BtsCI GGATG 4 cut(s) 42, 796, 970, 1009
BveI ACCTGC 1 cut(s) 1072
Cac8I GCNNGC 4 cut(s) 79, 156, 414, 503
CciI TCATGA 1 cut(s) 687
Cfr10I RCCGGY 1 cut(s) 494
Cfr13I GGNCC 2 cut(s) 623, 1120
Cfr42I CCGCGG 1 cut(s) 321
Csp6I GTAC 3 cut(s) 346, 550, 821
CviAII CATG 6 cut(s) 170, 477, 688, 773, 1136, 1247
CviQI GTAC 3 cut(s) 346, 550, 821
DdeI CTNAG 2 cut(s) 175, 767
DpnI GATC 5 cut(s) 137, 250, 393, 459, 1141
DpnII GATC 5 cut(s) 135, 248, 391, 457, 1139
DrdI GACNNNNNNGTC 1 cut(s) 807
DseDI GACNNNNNNGTC 1 cut(s) 807
EaeI YGGCCR 1 cut(s) 861
Eam1104I CTCTTC 2 cut(s) 88, 552
EarI CTCTTC 2 cut(s) 88, 552
Eco130I CCWWGG 3 cut(s) 231, 476, 1228
Eco147I AGGCCT 1 cut(s) 873
Eco32I GATATC 1 cut(s) 901
EcoRV GATATC 1 cut(s) 901
EcoT14I CCWWGG 3 cut(s) 231, 476, 1228
EcoT22I ATGCAT 1 cut(s) 121
ErhI CCWWGG 3 cut(s) 231, 476, 1228
Esp3I CGTCTC 1 cut(s) 644
FaeI CATG 6 cut(s) 173, 480, 691, 776, 1139, 1250
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 1 cut(s) 587
FatI CATG 6 cut(s) 169, 476, 687, 772, 1135, 1246
FbaI TGATCA 1 cut(s) 135
FokI GGATG 4 cut(s) 29, 803, 957, 996
FspBI CTAG 5 cut(s) 272, 432, 803, 914, 941
GsuI CTGGAG 1 cut(s) 728
HaeIII GGCC 4 cut(s) 624, 863, 873, 1122
HapII CCGG 1 cut(s) 495
Hin1I GRCGYC 1 cut(s) 576
Hin1II CATG 6 cut(s) 173, 480, 691, 776, 1139, 1250
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 4 cut(s) 309, 423, 528, 740
HpaII CCGG 1 cut(s) 495
HphI GGTGA 5 cut(s) 484, 991, 1082, 1178, 1244
Hpy166II GTNNAC 2 cut(s) 520, 1081
Hpy188I TCNGA 4 cut(s) 70, 178, 291, 667
Hpy188III TCNNGA 8 cut(s) 125, 389, 525, 649, 688, 914, 941, 1090
Hpy8I GTNNAC 2 cut(s) 520, 1081
HpyAV CCTTC 3 cut(s) 884, 953, 1061
HpyCH4III ACNGT 4 cut(s) 166, 385, 736, 820
HpyCH4IV ACGT 2 cut(s) 216, 576
HpyF10VI GCNNNNNNNGC 2 cut(s) 409, 543
HpyF3I CTNAG 2 cut(s) 175, 767
HpySE526I ACGT 2 cut(s) 216, 576
Hsp92I GRCGYC 1 cut(s) 576
Hsp92II CATG 6 cut(s) 173, 480, 691, 776, 1139, 1250
Ksp22I TGATCA 1 cut(s) 135
KspI CCGCGG 1 cut(s) 321
Kzo9I GATC 5 cut(s) 135, 248, 391, 457, 1139
LguI GCTCTTC 1 cut(s) 88
LmnI GCTCC 3 cut(s) 203, 763, 779
Lsp1109I GCAGC 7 cut(s) 103, 183, 387, 492, 510, 729, 918
LweI GCATC 1 cut(s) 1018
MaeI CTAG 5 cut(s) 272, 432, 803, 914, 941
MaeII ACGT 2 cut(s) 216, 576
MaeIII GTNAC 2 cut(s) 160, 437
MalI GATC 5 cut(s) 137, 250, 393, 459, 1141
MbiI CCGCTC 1 cut(s) 206
MboI GATC 5 cut(s) 135, 248, 391, 457, 1139
MboII GAAGA 5 cut(s) 75, 467, 569, 637, 1247
MfeI CAATTG 3 cut(s) 210, 255, 1259
MflI RGATCY 2 cut(s) 248, 457
MlsI TGGCCA 1 cut(s) 863
MluCI AATT 9 cut(s) 48, 72, 210, 255, 292, 404, 727, 933, 1259
MluNI TGGCCA 1 cut(s) 863
MlyI GAGTC 1 cut(s) 432
Mox20I TGGCCA 1 cut(s) 863
Mph1103I ATGCAT 1 cut(s) 121
MroXI GAANNNNTTC 2 cut(s) 296, 591
MscI TGGCCA 1 cut(s) 863
MseI TTAA 1 cut(s) 699
MslI CAYNNNNRTG 1 cut(s) 1014
Msp20I TGGCCA 1 cut(s) 863
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 1 cut(s) 495
MunI CAATTG 3 cut(s) 210, 255, 1259
Mva1269I GAATGC 2 cut(s) 121, 1254
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 2 cut(s) 409, 543
NcoI CCATGG 1 cut(s) 476
NdeII GATC 5 cut(s) 135, 248, 391, 457, 1139
NlaIII CATG 6 cut(s) 173, 480, 691, 776, 1139, 1250
NlaIV GGNNCC 2 cut(s) 357, 625
NmuCI GTSAC 2 cut(s) 160, 437
NsiI ATGCAT 1 cut(s) 121
NspI RCATGY 1 cut(s) 1250
NspV TTCGAA 1 cut(s) 484
PagI TCATGA 1 cut(s) 687
PceI AGGCCT 1 cut(s) 873
PciI ACATGT 1 cut(s) 1246
PciSI GCTCTTC 1 cut(s) 88
PctI GAATGC 2 cut(s) 121, 1254
PdmI GAANNNNTTC 2 cut(s) 296, 591
PfeI GAWTC 3 cut(s) 309, 528, 740
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
PscI ACATGT 1 cut(s) 1246
PspN4I GGNNCC 2 cut(s) 357, 625
PspPI GGNCC 2 cut(s) 623, 1120
PsrI GAACNNNNNNTAC 2 cut(s) 936, 968
PsuI RGATCY 2 cut(s) 248, 457
RsaI GTAC 3 cut(s) 347, 551, 822
RsaNI GTAC 3 cut(s) 346, 550, 821
RseI CAYNNNNRTG 1 cut(s) 1014
SacII CCGCGG 1 cut(s) 321
SapI GCTCTTC 1 cut(s) 88
SaqAI TTAA 1 cut(s) 699
Sau3AI GATC 5 cut(s) 135, 248, 391, 457, 1139
Sau96I GGNCC 2 cut(s) 623, 1120
SchI GAGTC 1 cut(s) 432
SfaNI GCATC 1 cut(s) 1018
Sfr303I CCGCGG 1 cut(s) 321
SfuI TTCGAA 1 cut(s) 484
SgrBI CCGCGG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 1014
SmlI CTYRAG 2 cut(s) 15, 338
SmoI CTYRAG 2 cut(s) 15, 338
Sse9I AATT 9 cut(s) 48, 72, 210, 255, 292, 404, 727, 933, 1259
SseBI AGGCCT 1 cut(s) 873
SsiI CCGC 5 cut(s) 206, 318, 320, 473, 515
SspMI CTAG 5 cut(s) 272, 432, 803, 914, 941
StuI AGGCCT 1 cut(s) 873
StyI CCWWGG 3 cut(s) 231, 476, 1228
TaaI ACNGT 4 cut(s) 166, 385, 736, 820
TaiI ACGT 2 cut(s) 219, 579
TaqI TCGA 3 cut(s) 484, 648, 731
TasI AATT 9 cut(s) 48, 72, 210, 255, 292, 404, 727, 933, 1259
TatI WGTACW 1 cut(s) 549
TauI GCSGC 3 cut(s) 209, 320, 475
TfiI GAWTC 3 cut(s) 309, 528, 740
Tru1I TTAA 1 cut(s) 699
Tru9I TTAA 1 cut(s) 699
TseFI GTSAC 2 cut(s) 160, 437
TseI GCWGC 7 cut(s) 91, 196, 400, 498, 505, 717, 906
Tsp45I GTSAC 2 cut(s) 160, 437
TspDTI ATGAA 4 cut(s) 158, 600, 676, 704
TspGWI ACGGA 2 cut(s) 163, 368
XapI RAATTY 1 cut(s) 727
XbaI TCTAGA 2 cut(s) 913, 940
XceI RCATGY 1 cut(s) 1250
XmnI GAANNNNTTC 2 cut(s) 296, 591
XspI CTAG 5 cut(s) 272, 432, 803, 914, 941
ZraI GACGTC 1 cut(s) 577
Zsp2I ATGCAT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.