AT5G01260

Starch binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002509
Physical Location & Seq
Forward (+)
105268 .. 107454
2187 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01260.1

Sequence Viewer

Length: 921 bp
ATGATTGGCATTGGAAGAACCCTAACTTCTTCTTCTCCATCTAAGATCATTCTCGAAGATCTTCCTCCTGGAATTTATCGCTATGGAACAGAGATATTCACTTCCGTTCCCAGGAAAACGTTCCATCACATCAAGTTTTTGCGTTTGGATTCTGCTCAGAGCAGCAGGATTCTTAAGCCGGTCCCGCTTCGTTCTTCGTCGATTAAGGATTCGCAGGTAAACGTAGAAGATGAAGAAATCGAAGCTTCTAACAAAACAGTTCGTGTAAGGTTCCAGTTGCGGAAAGAATGTGTCTTTGGGGAACATTTTTTCATTGTTGGTGATGATCCTGTGTTCGGTGGCCTTTGGGATCCAGAAACTGCCTTACCACTGAACTGGTCTGATGGCAATGTTTGGACTGTAGATTTGGATTTGCCTGTTGGAAGATTGGTTGAATTTAAGCTTTTATTGAAGGCACAGACAGGGGAGATTTTGTGGCAACCAGGTCCAAACCGGGCTCTTGAAACCTGGGAAACTAACAAAACTATCAGAATATGTGAAGACTGGGATAATGCTGATCTTCAAATGATGATTGAGGAAGATTTTGTGCCTTACACCAATATTTCGTCGATCGGTTCAGAAGATGAGGATGAAGTGCTTGGTAGTGTACAACAAAACTCTTCAGTAGTAGCTGTAGAGAATGCAGGATACGTGAGTGATGAGTCTGCCCAGAATTCGAGTTTCAGTATCCAAAGTGAGAAAACTATGGAACCTTCGAATGGAGCTTTGACTGCTCGGGAGGTGATCAAAGAAGCCATGTTTACTGAGGAAGAAAGTCCAGTTTTGGTTCCTGGATTGATTCCTCTGTCTGACTTGGATAATGAACAAGTGGAAGTTATTAATGAAGGTAAAGCAGAAACGTTCCCGGAGGTAATAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM_20

Protein Analysis

306

Amino Acids

34.18

Weight (kDa)

4.41

Isoelectric Point (pI)

54.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_20 PF00686 86 - 175 4.8e-17 Starch binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 205
AciI CCGC 2 cut(s) 185, 280
AclI AACGTT 2 cut(s) 119, 899
AclWI GGATC 3 cut(s) 320, 344, 357
AcsI RAATTY 3 cut(s) 72, 434, 712
AcuI CTGAAG 1 cut(s) 645
AfaI GTAC 1 cut(s) 648
AfiI CCNNNNNNNGG 3 cut(s) 111, 335, 758
AflII CTTAAG 1 cut(s) 173
AgsI TTSAA 4 cut(s) 434, 451, 503, 563
AjnI CCWGG 5 cut(s) 67, 110, 481, 506, 829
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AluBI AGCT 4 cut(s) 245, 442, 671, 764
AluI AGCT 4 cut(s) 245, 442, 671, 764
AlwI GGATC 3 cut(s) 320, 344, 357
AlwNI CAGNNNCTG 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 774
AoxI GGCC 1 cut(s) 340
ApeKI GCWGC 1 cut(s) 162
ApoI RAATTY 3 cut(s) 72, 434, 712
ArsI GACNNNNNNTTYG 2 cut(s) 388, 420
AseI ATTAAT 1 cut(s) 879
Asp700I GAANNNNTTC 2 cut(s) 60, 119
AspS9I GGNCC 2 cut(s) 181, 485
AsuC2I CCSGG 2 cut(s) 494, 905
AsuHPI GGTGA 2 cut(s) 332, 793
AsuII TTCGAA 1 cut(s) 755
AvaI CYCGRG 1 cut(s) 774
AvaII GGWCC 2 cut(s) 181, 485
BaeI ACNNNNGTAYC 2 cut(s) 679, 712
BamHI GGATCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 499
BbsI GAAGAC 1 cut(s) 546
BbvI GCAGC 1 cut(s) 174
BccI CCATC 3 cut(s) 46, 132, 377
BciT130I CCWGG 5 cut(s) 69, 112, 483, 508, 831
BciVI GTATCC 2 cut(s) 680, 737
BclI TGATCA 1 cut(s) 783
BcnI CCSGG 2 cut(s) 494, 905
BfmI CTRYAG 2 cut(s) 399, 672
BfrI CTTAAG 1 cut(s) 173
BfuAI ACCTGC 1 cut(s) 205
BfuI GTATCC 2 cut(s) 680, 737
BglII AGATCT 1 cut(s) 58
BisI GCNGC 1 cut(s) 163
BlsI GCNGC 1 cut(s) 164
Bme1390I CCNGG 7 cut(s) 69, 112, 483, 494, 508, 831, 905
Bme18I GGWCC 2 cut(s) 181, 485
BmeT110I CYCGRG 1 cut(s) 774
BmgT120I GGNCC 2 cut(s) 181, 485
BmiI GGNNCC 5 cut(s) 183, 272, 351, 750, 828
BmrFI CCNGG 7 cut(s) 69, 112, 483, 494, 508, 831, 905
BmrI ACTGGG 1 cut(s) 553
BmuI ACTGGG 1 cut(s) 553
BpiI GAAGAC 1 cut(s) 546
Bpu14I TTCGAA 1 cut(s) 755
BpuMI CCSGG 2 cut(s) 494, 905
BsaAI YACGTR 1 cut(s) 691
BsaJI CCNNGG 2 cut(s) 110, 507
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 335, 758
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 4 cut(s) 274, 380, 548, 818
Bse3DI GCAATG 1 cut(s) 394
BseBI CCWGG 5 cut(s) 69, 112, 483, 508, 831
BseDI CCNNGG 2 cut(s) 110, 507
BseGI GGATG 1 cut(s) 634
BseLI CCNNNNNNNGG 3 cut(s) 111, 335, 758
BseMI GCAATG 1 cut(s) 394
BseMII CTCAG 2 cut(s) 170, 795
BseNI ACTGG 4 cut(s) 274, 380, 548, 818
BseXI GCAGC 1 cut(s) 174
Bsh1285I CGRYCG 1 cut(s) 612
BshFI GGCC 1 cut(s) 342
BsiEI CGRYCG 1 cut(s) 612
BsiHKCI CYCGRG 1 cut(s) 774
BsiSI CCGG 3 cut(s) 179, 493, 905
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 3 cut(s) 111, 335, 758
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 1 cut(s) 685
BsnI GGCC 1 cut(s) 342
BsoBI CYCGRG 1 cut(s) 774
Bsp119I TTCGAA 1 cut(s) 755
Bsp1286I GDGCHC 1 cut(s) 499
Bsp1407I TGTACA 1 cut(s) 646
Bsp143I GATC 7 cut(s) 45, 58, 325, 349, 556, 609, 783
BspACI CCGC 2 cut(s) 185, 280
BspANI GGCC 1 cut(s) 342
BspCNI CTCAG 2 cut(s) 169, 796
BspLI GGNNCC 5 cut(s) 183, 272, 351, 750, 828
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 3 cut(s) 320, 344, 357
BspT104I TTCGAA 1 cut(s) 755
BspTI CTTAAG 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 394
BsrFI RCCGGY 1 cut(s) 178
BsrGI TGTACA 1 cut(s) 646
BsrI ACTGG 4 cut(s) 274, 380, 548, 818
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 110, 507
BssMI GATC 7 cut(s) 45, 58, 325, 349, 556, 609, 783
Bst2UI CCWGG 5 cut(s) 69, 112, 483, 508, 831
Bst4CI ACNGT 2 cut(s) 259, 400
Bst6I CTCTTC 1 cut(s) 664
BstAFI CTTAAG 1 cut(s) 173
BstAUI TGTACA 1 cut(s) 646
BstBAI YACGTR 1 cut(s) 691
BstBI TTCGAA 1 cut(s) 755
BstDEI CTNAG 3 cut(s) 42, 156, 804
BstF5I GGATG 1 cut(s) 634
BstKTI GATC 7 cut(s) 48, 61, 328, 352, 559, 612, 786
BstMBI GATC 7 cut(s) 45, 58, 325, 349, 556, 609, 783
BstMCI CGRYCG 1 cut(s) 612
BstMWI GCNNNNNNNGC 2 cut(s) 184, 770
BstNI CCWGG 5 cut(s) 69, 112, 483, 508, 831
BstSCI CCNGG 7 cut(s) 67, 110, 481, 492, 506, 829, 903
BstSFI CTRYAG 2 cut(s) 399, 672
BstV1I GCAGC 1 cut(s) 174
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 2 cut(s) 58, 349
BstXI CCANNNNNNTGG 1 cut(s) 375
BstYI RGATCY 2 cut(s) 58, 349
BsuI GTATCC 2 cut(s) 680, 737
BsuRI GGCC 1 cut(s) 342
BtsCI GGATG 1 cut(s) 634
BtsIMutI CAGTG 1 cut(s) 368
BveI ACCTGC 1 cut(s) 205
CaiI CAGNNNCTG 1 cut(s) 359
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 2 cut(s) 181, 485
CsiI ACCWGGT 1 cut(s) 481
Csp6I GTAC 1 cut(s) 647
CviAII CATG 1 cut(s) 796
CviJI RGCY 8 cut(s) 178, 245, 342, 442, 497, 671, 764, 794
CviKI_1 RGCY 8 cut(s) 178, 245, 342, 442, 497, 671, 764, 794
CviQI GTAC 1 cut(s) 647
DdeI CTNAG 3 cut(s) 42, 156, 804
DpnI GATC 7 cut(s) 47, 60, 327, 351, 558, 611, 785
DpnII GATC 7 cut(s) 45, 58, 325, 349, 556, 609, 783
Eam1104I CTCTTC 1 cut(s) 664
EarI CTCTTC 1 cut(s) 664
Eco24I GRGCYC 1 cut(s) 499
Eco47I GGWCC 2 cut(s) 181, 485
Eco57I CTGAAG 1 cut(s) 645
Eco88I CYCGRG 1 cut(s) 774
EcoRI GAATTC 1 cut(s) 712
EcoRII CCWGG 5 cut(s) 67, 110, 481, 506, 829
EcoT38I GRGCYC 1 cut(s) 499
FaeI CATG 1 cut(s) 799
FaiI YATR 4 cut(s) 84, 535, 746, 797
FaqI GGGAC 1 cut(s) 167
FatI CATG 1 cut(s) 795
FauI CCCGC 1 cut(s) 192
FbaI TGATCA 1 cut(s) 783
Fnu4HI GCNGC 1 cut(s) 163
FokI GGATG 1 cut(s) 641
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 1 cut(s) 163
GluI GCNGC 1 cut(s) 163
HaeIII GGCC 1 cut(s) 342
HapII CCGG 3 cut(s) 179, 493, 905
Hin1II CATG 1 cut(s) 799
HindIII AAGCTT 2 cut(s) 243, 440
HinfI GANTC 5 cut(s) 149, 169, 209, 701, 838
HpaII CCGG 3 cut(s) 179, 493, 905
HphI GGTGA 2 cut(s) 332, 793
Hpy166II GTNNAC 3 cut(s) 220, 647, 801
Hpy188I TCNGA 5 cut(s) 159, 382, 530, 619, 850
Hpy188III TCNNGA 4 cut(s) 53, 353, 500, 776
Hpy8I GTNNAC 3 cut(s) 220, 647, 801
Hpy99I CGWCG 2 cut(s) 202, 610
HpyAV CCTTC 3 cut(s) 445, 762, 878
HpyCH4III ACNGT 2 cut(s) 259, 400
HpyCH4IV ACGT 4 cut(s) 119, 222, 690, 899
HpyCH4V TGCA 1 cut(s) 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 184, 770
HpyF3I CTNAG 3 cut(s) 42, 156, 804
HpySE526I ACGT 4 cut(s) 119, 222, 690, 899
Hsp92II CATG 1 cut(s) 799
Ksp22I TGATCA 1 cut(s) 783
Kzo9I GATC 7 cut(s) 45, 58, 325, 349, 556, 609, 783
LmnI GCTCC 1 cut(s) 761
Lsp1109I GCAGC 1 cut(s) 174
MabI ACCWGGT 1 cut(s) 481
MaeII ACGT 4 cut(s) 119, 222, 690, 899
MalI GATC 7 cut(s) 47, 60, 327, 351, 558, 611, 785
MboI GATC 7 cut(s) 45, 58, 325, 349, 556, 609, 783
MflI RGATCY 2 cut(s) 58, 349
MhlI GDGCHC 1 cut(s) 499
MluCI AATT 3 cut(s) 72, 434, 712
MlyI GAGTC 1 cut(s) 710
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 7 cut(s) 75, 568, 619, 772, 799, 852, 901
MroXI GAANNNNTTC 2 cut(s) 60, 119
MseI TTAA 4 cut(s) 174, 204, 438, 879
MspCI CTTAAG 1 cut(s) 173
MspI CCGG 3 cut(s) 179, 493, 905
MspR9I CCNGG 7 cut(s) 69, 112, 483, 494, 508, 831, 905
Mva1269I GAATGC 1 cut(s) 685
MvaI CCWGG 5 cut(s) 69, 112, 483, 508, 831
MwoI GCNNNNNNNGC 2 cut(s) 184, 770
NciI CCSGG 2 cut(s) 494, 905
NdeII GATC 7 cut(s) 45, 58, 325, 349, 556, 609, 783
NlaIII CATG 1 cut(s) 799
NlaIV GGNNCC 5 cut(s) 183, 272, 351, 750, 828
NspV TTCGAA 1 cut(s) 755
PctI GAATGC 1 cut(s) 685
PdmI GAANNNNTTC 2 cut(s) 60, 119
PfeI GAWTC 4 cut(s) 149, 169, 209, 838
PfoI TCCNGGA 3 cut(s) 67, 829, 903
PkrI GCNGC 1 cut(s) 164
Ple19I CGATCG 1 cut(s) 612
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
Ppu21I YACGTR 1 cut(s) 691
PshBI ATTAAT 1 cut(s) 879
Psp1406I AACGTT 2 cut(s) 119, 899
Psp6I CCWGG 5 cut(s) 67, 110, 481, 506, 829
PspGI CCWGG 5 cut(s) 67, 110, 481, 506, 829
PspN4I GGNNCC 5 cut(s) 183, 272, 351, 750, 828
PspPI GGNCC 2 cut(s) 181, 485
PstNI CAGNNNCTG 1 cut(s) 359
PsuI RGATCY 2 cut(s) 58, 349
PvuI CGATCG 1 cut(s) 612
RsaI GTAC 1 cut(s) 648
RsaNI GTAC 1 cut(s) 647
SaqAI TTAA 4 cut(s) 174, 204, 438, 879
SatI GCNGC 1 cut(s) 163
Sau3AI GATC 7 cut(s) 45, 58, 325, 349, 556, 609, 783
Sau96I GGNCC 2 cut(s) 181, 485
SchI GAGTC 1 cut(s) 710
ScrFI CCNGG 7 cut(s) 69, 112, 483, 494, 508, 831, 905
SduI GDGCHC 1 cut(s) 499
SexAI ACCWGGT 1 cut(s) 481
SfcI CTRYAG 2 cut(s) 399, 672
SfuI TTCGAA 1 cut(s) 755
SinI GGWCC 2 cut(s) 181, 485
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 3 cut(s) 72, 434, 712
SsiI CCGC 2 cut(s) 185, 280
SspI AATATT 1 cut(s) 601
StyD4I CCNGG 7 cut(s) 67, 110, 481, 492, 506, 829, 903
TaaI ACNGT 2 cut(s) 259, 400
TaiI ACGT 4 cut(s) 122, 225, 693, 902
TaqI TCGA 6 cut(s) 54, 200, 240, 608, 716, 755
TasI AATT 3 cut(s) 72, 434, 712
TatI WGTACW 1 cut(s) 646
TfiI GAWTC 4 cut(s) 149, 169, 209, 838
Tru1I TTAA 4 cut(s) 174, 204, 438, 879
Tru9I TTAA 4 cut(s) 174, 204, 438, 879
TscAI CASTG 1 cut(s) 375
TseI GCWGC 1 cut(s) 162
TspDTI ATGAA 5 cut(s) 246, 301, 645, 876, 897
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 1 cut(s) 375
Vha464I CTTAAG 1 cut(s) 173
VpaK11BI GGWCC 2 cut(s) 181, 485
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 3 cut(s) 72, 434, 712
XmnI GAANNNNTTC 2 cut(s) 60, 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.