FvH4_6g11650

Starch binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002509
Physical Location & Seq
Reverse (-)
6976490 .. 6980047
3558 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g11650.t3

Sequence Viewer

Length: 1503 bp
ATGAAAACGCTGGCGAGTCCGAGTTCGAGGGTAGTGTTGGAAATTTACAGGGACAGAGAGGCTGGCTTGGTGAACAGGGTTGCAGAAGCTTCTTGCTTTTTCAGGCCTCACAAGAAAAATGGAGACTTTCGGTTCTTGAATTTGGTGGCGTTTCTGCAACAGAGGAAGCCCATCAAGCCTCTTGTTTGTCTCTCATCGAAAACCCAGGCAGAATTGGAGACTCCAGATGCTCAAATTCAAGATTCATATCCAATAAAAACTATGCATGTCAAGTTCCAGTTGCAGAAAGAGTGCTCCTTTGGTCAGCAGTTCCTCATAGTAGGGGATGATCCTATTTTGGGTTCATGGGACCCTGAGAGGGCGATACCAATGGACTGGTCTGATGGGAATGTGTGGTCTGTTGAGCTAGATATACCAGTTGGAAAATCAATCCAGTTCAAGTTCATACTAACAGGAGACGCAGGGAATTTTTTGTGGCAACCTGGCCCGGATCGAATTTTCCAAACCTGGGAAACTGAAAACACAATCACTGTTTTTGAAGATTGGGGAGATGCTGAACTTCAGAAGATAACAGAGGAAGCGCAAAACTATCACCAGGTTGAGGATTCTAATATCAACTCAGATGTATTGATTCCAGAGGCTGACAACTTGACATTTCCAGAAGAGGAGCCAGTGTTCAACATAAACTTGGAGTCGGCCAATGTTGATAGTTACACTGATCCAGCACAGATCCCGTTAGTGGAATCATACGAGGAACAAACTGTTACTGAGTCTGTCTCACAAGAGAAGCACAAAACTGTTGTTGCTGAGAACATAACACCAAAATGGCCAAAGAAAATGATGGAACCATATGAGCAACATATGGACGCAAGAACTAATGCCCCAAGTGAGGATTCTGCAGCCATCCCAAACGAGATGATGGTGGCACAGAACATTTATGGAAATAATGGTAGAGATGCAAAAGCAAGGAACCTGGCAATCTCAAACATTGAGGGAAGCCTAATGAACTATGAGGAAGAAGGACCTGTTCTGGTACCTGGCTTGACTCGTGCGCCAGCTGTTCAAGCTGAAGAACCAAACACAGAGGAAGTTGAGAAACCAATGACCTTTGATGGCTTGGTTGGAGATTATGAAGCTGCAGAACTGAATATGCCAGAGCTAGAAGAAAAGCAAGAACCCTACAGTGAATCATCTCAAGCAGAAACAATGGACATGCTCAATGGTAATGTAGAGAAGTTTGACGAAGAACTCAAGCAAAAGCCTCAACCAGCTGAAAGGGAAAATCAGTCCAAGCCTGACCTGCCCAATGGCTCACCTCAAGCAGAAACCAATGACATGTTCAACAATGATGAAGTGAGTTTGGATGATGAACTTGAGCAAAAGGCTCAAACTGCTGAAACTGCAGAACAGTTTGCCTCAGAGAAAGTCCATGATGTCTGGCACAATGACATGCAATGGGGTCGTAAACTACTGCAGAAACTGATAAATTTCAGATTGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM_20

Protein Analysis

501

Amino Acids

56.75

Weight (kDa)

4.41

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_20 PF00686 2 - 89 9.1e-21 Starch binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1308
Acc65I GGTACC 1 cut(s) 1033
AccB1I GGYRCC 1 cut(s) 1033
AclWI GGATC 4 cut(s) 323, 498, 713, 724
AcoI YGGCCR 2 cut(s) 696, 827
AcsI RAATTY 6 cut(s) 42, 139, 234, 466, 495, 1486
AcuI CTGAAG 2 cut(s) 545, 1089
AfaI GTAC 1 cut(s) 1035
AfiI CCNNNNNNNGG 6 cut(s) 338, 358, 508, 601, 739, 889
AflIII ACRYGT 1 cut(s) 1335
AgsI TTSAA 7 cut(s) 139, 239, 439, 539, 679, 1064, 1342
AjnI CCWGG 6 cut(s) 204, 481, 506, 594, 972, 1036
AjuI GAANNNNNNNTTGG 4 cut(s) 877, 909, 1322, 1354
AloI GAACNNNNNNTCC 2 cut(s) 659, 691
AluBI AGCT 7 cut(s) 89, 406, 1058, 1067, 1136, 1159, 1271
AluI AGCT 7 cut(s) 89, 406, 1058, 1067, 1136, 1159, 1271
Alw21I GWGCWC 1 cut(s) 296
Alw26I GTCTC 5 cut(s) 117, 194, 212, 450, 781
AlwI GGATC 4 cut(s) 323, 498, 713, 724
AlwNI CAGNNNCTG 2 cut(s) 641, 1480
AoxI GGCC 4 cut(s) 104, 484, 696, 827
ApeKI GCWGC 2 cut(s) 899, 1136
ApoI RAATTY 6 cut(s) 42, 139, 234, 466, 495, 1486
Asp718I GGTACC 1 cut(s) 1033
AspLEI GCGC 2 cut(s) 583, 1054
AspS9I GGNCC 3 cut(s) 349, 485, 1022
AsuC2I CCSGG 1 cut(s) 488
AsuHPI GGTGA 3 cut(s) 82, 584, 1305
AvaII GGWCC 2 cut(s) 349, 1022
BalI TGGCCA 1 cut(s) 829
BanI GGYRCC 1 cut(s) 1033
BauI CACGAG 1 cut(s) 1047
Bbv12I GWGCWC 1 cut(s) 296
BbvI GCAGC 2 cut(s) 911, 1123
BccI CCATC 6 cut(s) 179, 377, 835, 911, 913, 1106
BcgI CGANNNNNNTGC 2 cut(s) 1442, 1476
BciT130I CCWGG 6 cut(s) 206, 483, 508, 596, 974, 1038
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 5 cut(s) 117, 194, 212, 450, 781
BfaI CTAG 2 cut(s) 407, 1160
BfmI CTRYAG 5 cut(s) 897, 1137, 1180, 1401, 1472
BfuAI ACCTGC 1 cut(s) 1308
BisI GCNGC 2 cut(s) 900, 1137
BlsI GCNGC 2 cut(s) 901, 1138
Bme1390I CCNGG 7 cut(s) 206, 483, 488, 508, 596, 974, 1038
Bme18I GGWCC 2 cut(s) 349, 1022
BmgT120I GGNCC 3 cut(s) 349, 485, 1022
BmiI GGNNCC 6 cut(s) 350, 351, 669, 846, 971, 1035
BmrFI CCNGG 7 cut(s) 206, 483, 488, 508, 596, 974, 1038
BmsI GCATC 3 cut(s) 217, 541, 946
BplI GAGNNNNNCTC 2 cut(s) 761, 793
BpmI CTGGAG 1 cut(s) 207
BpuEI CTTGAG 4 cut(s) 1179, 1235, 1302, 1394
BpuMI CCSGG 1 cut(s) 488
BsaBI GATNNNNATC 1 cut(s) 246
BsaJI CCNNGG 2 cut(s) 204, 507
BsaXI ACNNNNNCTCC 2 cut(s) 659, 689
Bsc4I CCNNNNNNNGG 6 cut(s) 338, 358, 508, 601, 739, 889
Bse1I ACTGG 5 cut(s) 277, 380, 416, 433, 671
Bse3DI GCAATG 1 cut(s) 1460
Bse8I GATNNNNATC 1 cut(s) 246
BseBI CCWGG 6 cut(s) 206, 483, 508, 596, 974, 1038
BseDI CCNNGG 2 cut(s) 204, 507
BseGI GGATG 3 cut(s) 331, 903, 1369
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 6 cut(s) 338, 358, 508, 601, 739, 889
BseMI GCAATG 1 cut(s) 1460
BseMII CTCAG 5 cut(s) 345, 633, 759, 798, 1431
BseNI ACTGG 5 cut(s) 277, 380, 416, 433, 671
BseRI GAGGAG 1 cut(s) 680
BseXI GCAGC 2 cut(s) 911, 1123
BshFI GGCC 4 cut(s) 106, 486, 698, 829
BshNI GGYRCC 1 cut(s) 1033
BsiHKAI GWGCWC 1 cut(s) 296
BsiSI CCGG 1 cut(s) 488
BslFI GGGAC 2 cut(s) 65, 362
BslI CCNNNNNNNGG 6 cut(s) 338, 358, 508, 601, 739, 889
BsmAI GTCTC 5 cut(s) 117, 194, 212, 450, 781
BsmBI CGTCTC 1 cut(s) 450
BsmFI GGGAC 2 cut(s) 65, 362
BsnI GGCC 4 cut(s) 106, 486, 698, 829
Bsp1286I GDGCHC 1 cut(s) 296
Bsp143I GATC 4 cut(s) 328, 490, 718, 729
BspANI GGCC 4 cut(s) 106, 486, 698, 829
BspCNI CTCAG 5 cut(s) 346, 632, 760, 799, 1430
BspLI GGNNCC 6 cut(s) 350, 351, 669, 846, 971, 1035
BspMAI CTGCAG 4 cut(s) 901, 1141, 1405, 1476
BspMI ACCTGC 1 cut(s) 1308
BspPI GGATC 4 cut(s) 323, 498, 713, 724
BspT107I GGYRCC 1 cut(s) 1033
BsrDI GCAATG 1 cut(s) 1460
BsrI ACTGG 5 cut(s) 277, 380, 416, 433, 671
BssECI CCNNGG 2 cut(s) 204, 507
BssMI GATC 4 cut(s) 328, 490, 718, 729
BssSI CACGAG 1 cut(s) 1047
Bst2BI CACGAG 1 cut(s) 1047
Bst2UI CCWGG 6 cut(s) 206, 483, 508, 596, 974, 1038
Bst4CI ACNGT 5 cut(s) 532, 763, 799, 1184, 1410
Bst6I CTCTTC 1 cut(s) 657
BstC8I GCNNGC 3 cut(s) 12, 64, 1056
BstDEI CTNAG 6 cut(s) 354, 619, 768, 807, 1417, 1500
BstF5I GGATG 3 cut(s) 331, 903, 1369
BstHHI GCGC 2 cut(s) 583, 1054
BstKTI GATC 4 cut(s) 331, 493, 721, 732
BstMAI GTCTC 5 cut(s) 117, 194, 212, 450, 781
BstMBI GATC 4 cut(s) 328, 490, 718, 729
BstMWI GCNNNNNNNGC 5 cut(s) 175, 1064, 1300, 1391, 1400
BstNI CCWGG 6 cut(s) 206, 483, 508, 596, 974, 1038
BstNSI RCATGY 4 cut(s) 269, 1216, 1339, 1453
BstSCI CCNGG 7 cut(s) 204, 481, 486, 506, 594, 972, 1036
BstSFI CTRYAG 5 cut(s) 897, 1137, 1180, 1401, 1472
BstV1I GCAGC 2 cut(s) 911, 1123
BstX2I RGATCY 1 cut(s) 729
BstXI CCANNNNNNTGG 1 cut(s) 375
BstYI RGATCY 1 cut(s) 729
BsuRI GGCC 4 cut(s) 106, 486, 698, 829
BtsCI GGATG 3 cut(s) 331, 903, 1369
BtsIMutI CAGTG 4 cut(s) 528, 678, 714, 1189
BveI ACCTGC 1 cut(s) 1308
Cac8I GCNNGC 3 cut(s) 12, 64, 1056
CaiI CAGNNNCTG 2 cut(s) 641, 1480
CfoI GCGC 2 cut(s) 583, 1054
Cfr13I GGNCC 3 cut(s) 349, 485, 1022
CseI GACGC 2 cut(s) 467, 875
CsiI ACCWGGT 1 cut(s) 594
Csp6I GTAC 1 cut(s) 1034
CviAII CATG 6 cut(s) 266, 345, 1213, 1336, 1430, 1450
CviQI GTAC 1 cut(s) 1034
DdeI CTNAG 6 cut(s) 354, 619, 768, 807, 1417, 1500
DpnI GATC 4 cut(s) 330, 492, 720, 731
DpnII GATC 4 cut(s) 328, 490, 718, 729
EaeI YGGCCR 2 cut(s) 696, 827
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
Eco147I AGGCCT 1 cut(s) 106
Eco47I GGWCC 2 cut(s) 349, 1022
Eco57I CTGAAG 2 cut(s) 545, 1089
EcoO109I RGGNCCY 2 cut(s) 349, 1022
EcoRII CCWGG 6 cut(s) 204, 481, 506, 594, 972, 1036
EcoT22I ATGCAT 1 cut(s) 267
Esp3I CGTCTC 1 cut(s) 450
FaeI CATG 6 cut(s) 269, 348, 1216, 1339, 1433, 1453
FaqI GGGAC 2 cut(s) 65, 362
FatI CATG 6 cut(s) 265, 344, 1212, 1335, 1429, 1449
FauNDI CATATG 2 cut(s) 850, 861
Fnu4HI GCNGC 2 cut(s) 900, 1137
FokI GGATG 3 cut(s) 338, 890, 1376
Fsp4HI GCNGC 2 cut(s) 900, 1137
FspBI CTAG 2 cut(s) 407, 1160
GlaI GCGC 2 cut(s) 582, 1053
GluI GCNGC 2 cut(s) 900, 1137
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 4 cut(s) 106, 486, 698, 829
HapII CCGG 1 cut(s) 488
HgaI GACGC 2 cut(s) 467, 875
HhaI GCGC 2 cut(s) 583, 1054
Hin1II CATG 6 cut(s) 269, 348, 1216, 1339, 1433, 1453
Hin6I GCGC 2 cut(s) 581, 1052
HinP1I GCGC 2 cut(s) 581, 1052
HindIII AAGCTT 1 cut(s) 87
HpaII CCGG 1 cut(s) 488
HphI GGTGA 3 cut(s) 82, 584, 1305
Hpy166II GTNNAC 2 cut(s) 73, 1466
Hpy188I TCNGA 6 cut(s) 21, 382, 564, 622, 1420, 1493
Hpy188III TCNNGA 5 cut(s) 136, 224, 239, 635, 659
Hpy8I GTNNAC 2 cut(s) 73, 1466
HpyAV CCTTC 1 cut(s) 1013
HpyCH4III ACNGT 5 cut(s) 532, 763, 799, 1184, 1410
HpyF10VI GCNNNNNNNGC 5 cut(s) 175, 1064, 1300, 1391, 1400
HpyF3I CTNAG 6 cut(s) 354, 619, 768, 807, 1417, 1500
Hsp92II CATG 6 cut(s) 269, 348, 1216, 1339, 1433, 1453
HspAI GCGC 2 cut(s) 581, 1052
KflI GGGWCCC 1 cut(s) 349
KpnI GGTACC 1 cut(s) 1037
Kzo9I GATC 4 cut(s) 328, 490, 718, 729
LmnI GCTCC 2 cut(s) 299, 667
Lsp1109I GCAGC 2 cut(s) 911, 1123
LweI GCATC 3 cut(s) 217, 541, 946
MabI ACCWGGT 1 cut(s) 594
MaeI CTAG 2 cut(s) 407, 1160
MaeIII GTNAC 2 cut(s) 710, 763
MalI GATC 4 cut(s) 330, 492, 720, 731
MboI GATC 4 cut(s) 328, 490, 718, 729
MboII GAAGA 7 cut(s) 551, 577, 674, 1028, 1082, 1175, 1256
MflI RGATCY 1 cut(s) 729
MhlI GDGCHC 1 cut(s) 296
MlsI TGGCCA 1 cut(s) 829
MluCI AATT 7 cut(s) 42, 139, 212, 234, 466, 495, 1486
MluNI TGGCCA 1 cut(s) 829
MlyI GAGTC 5 cut(s) 25, 214, 701, 779, 1039
MmeI TCCRAC 3 cut(s) 18, 400, 1102
Mox20I TGGCCA 1 cut(s) 829
Mph1103I ATGCAT 1 cut(s) 267
MscI TGGCCA 1 cut(s) 829
MslI CAYNNNNRTG 1 cut(s) 823
Msp20I TGGCCA 1 cut(s) 829
MspA1I CMGCKG 2 cut(s) 1058, 1271
MspI CCGG 1 cut(s) 488
MspR9I CCNGG 7 cut(s) 206, 483, 488, 508, 596, 974, 1038
MvaI CCWGG 6 cut(s) 206, 483, 508, 596, 974, 1038
MwoI GCNNNNNNNGC 5 cut(s) 175, 1064, 1300, 1391, 1400
NciI CCSGG 1 cut(s) 488
NdeI CATATG 2 cut(s) 850, 861
NdeII GATC 4 cut(s) 328, 490, 718, 729
NlaIII CATG 6 cut(s) 269, 348, 1216, 1339, 1433, 1453
NlaIV GGNNCC 6 cut(s) 350, 351, 669, 846, 971, 1035
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 4 cut(s) 269, 1216, 1339, 1453
PceI AGGCCT 1 cut(s) 106
PciI ACATGT 1 cut(s) 1335
PfeI GAWTC 6 cut(s) 242, 605, 631, 743, 893, 1187
PkrI GCNGC 2 cut(s) 901, 1138
PleI GAGTC 5 cut(s) 24, 214, 700, 778, 1039
PpsI GAGTC 5 cut(s) 24, 214, 700, 778, 1039
PpuMI RGGWCCY 2 cut(s) 349, 1022
PscI ACATGT 1 cut(s) 1335
Psp5II RGGWCCY 2 cut(s) 349, 1022
Psp6I CCWGG 6 cut(s) 204, 481, 506, 594, 972, 1036
PspGI CCWGG 6 cut(s) 204, 481, 506, 594, 972, 1036
PspN4I GGNNCC 6 cut(s) 350, 351, 669, 846, 971, 1035
PspPI GGNCC 3 cut(s) 349, 485, 1022
PspPPI RGGWCCY 2 cut(s) 349, 1022
PstI CTGCAG 4 cut(s) 901, 1141, 1405, 1476
PstNI CAGNNNCTG 2 cut(s) 641, 1480
PsuI RGATCY 1 cut(s) 729
PvuII CAGCTG 2 cut(s) 1058, 1271
RsaI GTAC 1 cut(s) 1035
RsaNI GTAC 1 cut(s) 1034
RseI CAYNNNNRTG 1 cut(s) 823
SatI GCNGC 2 cut(s) 900, 1137
Sau3AI GATC 4 cut(s) 328, 490, 718, 729
Sau96I GGNCC 3 cut(s) 349, 485, 1022
SchI GAGTC 5 cut(s) 25, 214, 701, 779, 1039
ScrFI CCNGG 7 cut(s) 206, 483, 488, 508, 596, 974, 1038
SduI GDGCHC 1 cut(s) 296
SexAI ACCWGGT 1 cut(s) 594
SfaNI GCATC 3 cut(s) 217, 541, 946
SfcI CTRYAG 5 cut(s) 897, 1137, 1180, 1401, 1472
SinI GGWCC 2 cut(s) 349, 1022
SmiMI CAYNNNNRTG 1 cut(s) 823
SmlI CTYRAG 4 cut(s) 1194, 1250, 1317, 1373
SmoI CTYRAG 4 cut(s) 1194, 1250, 1317, 1373
Sse9I AATT 7 cut(s) 42, 139, 212, 234, 466, 495, 1486
SseBI AGGCCT 1 cut(s) 106
SspMI CTAG 2 cut(s) 407, 1160
StuI AGGCCT 1 cut(s) 106
StyD4I CCNGG 7 cut(s) 204, 481, 486, 506, 594, 972, 1036
TaaI ACNGT 5 cut(s) 532, 763, 799, 1184, 1410
TaqI TCGA 3 cut(s) 26, 197, 493
TasI AATT 7 cut(s) 42, 139, 212, 234, 466, 495, 1486
TfiI GAWTC 6 cut(s) 242, 605, 631, 743, 893, 1187
TscAI CASTG 4 cut(s) 535, 678, 721, 1189
TseI GCWGC 2 cut(s) 899, 1136
TspDTI ATGAA 8 cut(s) 17, 234, 333, 433, 1019, 1146, 1365, 1383
TspRI CASTG 4 cut(s) 535, 678, 721, 1189
VpaK11BI GGWCC 2 cut(s) 349, 1022
XapI RAATTY 6 cut(s) 42, 139, 234, 466, 495, 1486
XceI RCATGY 4 cut(s) 269, 1216, 1339, 1453
XspI CTAG 2 cut(s) 407, 1160
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.