Rw5G042290

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002509
Physical Location & Seq
Forward (+)
74160020 .. 74161606
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042290.1

Sequence Viewer

Length: 996 bp
ATGGAAGCCTTTGCAAGATCTTCCACAACGTTGTTTGCAAAGGCTAATACTAACAAGCGTCCATCGCCTGTAAAAGCTCTTCTGAGTCAATCTAGCATTTGCTTTGTAAGGGTTTGGAATTCAAATGCAAATGTTCATCATCATACTTCTTTGCTTAGGCACAAAGCTATTCGATCGGTTTCCTCATCTGAAACAAGGGTGTTAAGGAACCCAGACTCAGCAGGAATTGGGATCGAACCAGCAGCGCCATCAAAAACCGTTCGTGTCCAATTCCAATTACACAAGAAGTGCCGCTTCGGTGATAATTTTCTCTTAGTAGGGAATGAACCTATCATTGGACAGTGGAACCCTTCAAGTGCTATACCCCTTAACTGGTCAGATGGAAACATTTGGACAGTTGACCTGGACATACCAATTGACAGAGCAATCCAGTTCAAGTTTATACTAAAGAAAAGCACAGGAGATCTGTTGTGGCAACCTGGTCCAGACCGAATTCTTCACACCTGGGATACCAACAGCACTATAACCATAGCCGAAGACTGGAAAAATGCCAAACTTCAGAAATTAACTGAAAAGAAAATTGTCAGTCAAAACGAGACGCTACTTACAGACCAGGCTTATAGCATACCAACTTATTATAGCAACAAGAATGAGTTTGCTATCAAAGAGAGGGCGTTTGGTTCTGAAAAGGGAACTCTGCTTCAGGAAAGCCAAGCAGTTTGCATTGCAGATGACGGTGTTTGCATCATGACAAGACCAGCATCCTTAGGAAGCATGGATGAAGATGAATACTGTGAAGTTGAATATTATGACGATGAATATTACGTTGATGACTGTGAATATGAAGATAGATATGAAGATGAAGACATCATGTTTACTTATGAAGGAGGCCGTGTTCTCGTGCCTGGTTTAGTACAAGGGGTAACTGGAAAAACCATTGATATCAGTGCTGATGGCTCGATTGTGATTGATGCGAGAGGGGAACACAATTCTTAA

Protein Analysis

331

Amino Acids

37.15

Weight (kDa)

5.12

Isoelectric Point (pI)

35.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_20 PF00686 86 - 174 9.8e-20 Starch binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 292
AclI AACGTT 1 cut(s) 29
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 2 cut(s) 118, 492
AcuI CTGAAG 2 cut(s) 542, 686
AfaI GTAC 1 cut(s) 915
AfiI CCNNNNNNNGG 3 cut(s) 335, 372, 540
AgsI TTSAA 4 cut(s) 123, 354, 436, 803
AjnI CCWGG 5 cut(s) 402, 478, 503, 612, 904
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AloI GAACNNNNNNTCC 2 cut(s) 879, 911
AluBI AGCT 2 cut(s) 77, 167
AluI AGCT 2 cut(s) 77, 167
Alw26I GTCTC 1 cut(s) 590
AlwI GGATC 1 cut(s) 239
AoxI GGCC 1 cut(s) 889
ApeKI GCWGC 1 cut(s) 242
ApoI RAATTY 2 cut(s) 118, 492
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 482
AsuHPI GGTGA 1 cut(s) 311
AvaII GGWCC 1 cut(s) 482
AxyI CCTNAGG 1 cut(s) 766
BarI GAAGNNNNNNTAC 2 cut(s) 63, 95
BauI CACGAG 1 cut(s) 899
BbsI GAAGAC 2 cut(s) 543, 870
BbvI GCAGC 1 cut(s) 254
BccI CCATC 4 cut(s) 70, 256, 374, 947
BceAI ACGGC 1 cut(s) 876
BciT130I CCWGG 5 cut(s) 404, 480, 505, 614, 906
BciVI GTATCC 1 cut(s) 502
BcoDI GTCTC 1 cut(s) 590
BfaI CTAG 1 cut(s) 93
BfoI RGCGCY 1 cut(s) 248
BfuI GTATCC 1 cut(s) 502
BglII AGATCT 2 cut(s) 17, 463
BisI GCNGC 2 cut(s) 243, 292
BlsI GCNGC 2 cut(s) 244, 293
Bme1390I CCNGG 5 cut(s) 404, 480, 505, 614, 906
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 1 cut(s) 482
BmiI GGNNCC 2 cut(s) 209, 347
BmrFI CCNGG 5 cut(s) 404, 480, 505, 614, 906
BmsI GCATC 3 cut(s) 753, 770, 961
BpiI GAAGAC 2 cut(s) 543, 870
Bpu10I CCTNAGC 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 504
BsaXI ACNNNNNCTCC 2 cut(s) 879, 909
Bsc4I CCNNNNNNNGG 3 cut(s) 335, 372, 540
Bse1I ACTGG 4 cut(s) 377, 430, 545, 931
Bse21I CCTNAGG 1 cut(s) 766
Bse3DI GCAATG 1 cut(s) 723
BseBI CCWGG 5 cut(s) 404, 480, 505, 614, 906
BseDI CCNNGG 1 cut(s) 504
BseGI GGATG 2 cut(s) 761, 784
BseLI CCNNNNNNNGG 3 cut(s) 335, 372, 540
BseMI GCAATG 1 cut(s) 723
BseMII CTCAG 2 cut(s) 74, 231
BseNI ACTGG 4 cut(s) 377, 430, 545, 931
BseXI GCAGC 1 cut(s) 254
Bsh1285I CGRYCG 1 cut(s) 176
BshFI GGCC 1 cut(s) 891
BsiEI CGRYCG 1 cut(s) 176
BslI CCNNNNNNNGG 3 cut(s) 335, 372, 540
BsmAI GTCTC 1 cut(s) 590
BsmBI CGTCTC 1 cut(s) 590
BsnI GGCC 1 cut(s) 891
Bsp143I GATC 4 cut(s) 17, 173, 231, 463
BspACI CCGC 1 cut(s) 292
BspANI GGCC 1 cut(s) 891
BspCNI CTCAG 2 cut(s) 75, 230
BspHI TCATGA 1 cut(s) 747
BspLI GGNNCC 2 cut(s) 209, 347
BspPI GGATC 1 cut(s) 239
BspQI GCTCTTC 1 cut(s) 84
BsrDI GCAATG 1 cut(s) 723
BsrI ACTGG 4 cut(s) 377, 430, 545, 931
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 4 cut(s) 17, 173, 231, 463
BssSI CACGAG 1 cut(s) 899
Bst2BI CACGAG 1 cut(s) 899
Bst2UI CCWGG 5 cut(s) 404, 480, 505, 614, 906
Bst4CI ACNGT 6 cut(s) 259, 342, 397, 737, 794, 836
Bst6I CTCTTC 1 cut(s) 84
BstDEI CTNAG 5 cut(s) 83, 155, 217, 313, 766
BstF5I GGATG 2 cut(s) 761, 784
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 4 cut(s) 20, 176, 234, 466
BstMAI GTCTC 1 cut(s) 590
BstMBI GATC 4 cut(s) 17, 173, 231, 463
BstMCI CGRYCG 1 cut(s) 176
BstMWI GCNNNNNNNGC 1 cut(s) 64
BstNI CCWGG 5 cut(s) 404, 480, 505, 614, 906
BstSCI CCNGG 5 cut(s) 402, 478, 503, 612, 904
BstV1I GCAGC 1 cut(s) 254
BstV2I GAAGAC 2 cut(s) 543, 870
BstX2I RGATCY 2 cut(s) 17, 463
BstYI RGATCY 2 cut(s) 17, 463
Bsu36I CCTNAGG 1 cut(s) 766
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 1 cut(s) 891
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 2 cut(s) 761, 784
BtsIMutI CAGTG 2 cut(s) 347, 952
CciI TCATGA 1 cut(s) 747
CfoI GCGC 1 cut(s) 247
Cfr13I GGNCC 1 cut(s) 482
CseI GACGC 2 cut(s) 47, 607
CsiI ACCWGGT 1 cut(s) 478
Csp6I GTAC 1 cut(s) 914
CviAII CATG 3 cut(s) 748, 775, 871
CviJI RGCY 9 cut(s) 8, 44, 77, 167, 533, 617, 711, 891, 957
CviKI_1 RGCY 9 cut(s) 8, 44, 77, 167, 533, 617, 711, 891, 957
CviQI GTAC 1 cut(s) 914
DdeI CTNAG 5 cut(s) 83, 155, 217, 313, 766
DpnI GATC 4 cut(s) 19, 175, 233, 465
DpnII GATC 4 cut(s) 17, 173, 231, 463
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco32I GATATC 1 cut(s) 943
Eco47I GGWCC 1 cut(s) 482
Eco57I CTGAAG 2 cut(s) 542, 686
Eco81I CCTNAGG 1 cut(s) 766
EcoRI GAATTC 2 cut(s) 118, 492
EcoRII CCWGG 5 cut(s) 402, 478, 503, 612, 904
EcoRV GATATC 1 cut(s) 943
Esp3I CGTCTC 1 cut(s) 590
FaeI CATG 3 cut(s) 751, 778, 874
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FatI CATG 3 cut(s) 747, 774, 870
Fnu4HI GCNGC 2 cut(s) 243, 292
FokI GGATG 2 cut(s) 748, 791
Fsp4HI GCNGC 2 cut(s) 243, 292
FspBI CTAG 1 cut(s) 93
GlaI GCGC 1 cut(s) 246
GluI GCNGC 2 cut(s) 243, 292
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 1 cut(s) 891
HgaI GACGC 2 cut(s) 47, 607
HhaI GCGC 1 cut(s) 247
Hin1II CATG 3 cut(s) 751, 778, 874
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HinfI GANTC 2 cut(s) 85, 215
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 2 cut(s) 400, 876
Hpy188I TCNGA 5 cut(s) 84, 190, 379, 561, 685
Hpy188III TCNNGA 3 cut(s) 485, 704, 748
Hpy8I GTNNAC 2 cut(s) 400, 876
HpyAV CCTTC 2 cut(s) 360, 878
HpyCH4III ACNGT 6 cut(s) 259, 342, 397, 737, 794, 836
HpyCH4IV ACGT 2 cut(s) 29, 825
HpyCH4V TGCA 6 cut(s) 14, 38, 128, 723, 728, 744
HpyF10VI GCNNNNNNNGC 1 cut(s) 64
HpyF3I CTNAG 5 cut(s) 83, 155, 217, 313, 766
HpySE526I ACGT 2 cut(s) 29, 825
Hsp92II CATG 3 cut(s) 751, 778, 874
HspAI GCGC 1 cut(s) 245
Kzo9I GATC 4 cut(s) 17, 173, 231, 463
LguI GCTCTTC 1 cut(s) 84
Lsp1109I GCAGC 1 cut(s) 254
LweI GCATC 3 cut(s) 753, 770, 961
MabI ACCWGGT 1 cut(s) 478
MaeI CTAG 1 cut(s) 93
MaeII ACGT 2 cut(s) 29, 825
MaeIII GTNAC 1 cut(s) 922
MalI GATC 4 cut(s) 19, 175, 233, 465
MboI GATC 4 cut(s) 17, 173, 231, 463
MboII GAAGA 8 cut(s) 12, 71, 488, 548, 794, 857, 869, 875
MfeI CAATTG 1 cut(s) 414
MflI RGATCY 2 cut(s) 17, 463
MlyI GAGTC 2 cut(s) 94, 209
MnlI CCTC 4 cut(s) 193, 663, 881, 971
MseI TTAA 4 cut(s) 203, 369, 566, 994
MspR9I CCNGG 5 cut(s) 404, 480, 505, 614, 906
MunI CAATTG 1 cut(s) 414
MvaI CCWGG 5 cut(s) 404, 480, 505, 614, 906
MwoI GCNNNNNNNGC 1 cut(s) 64
NdeII GATC 4 cut(s) 17, 173, 231, 463
NlaIII CATG 3 cut(s) 751, 778, 874
NlaIV GGNNCC 2 cut(s) 209, 347
PagI TCATGA 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 84
PkrI GCNGC 2 cut(s) 244, 293
Ple19I CGATCG 1 cut(s) 176
PleI GAGTC 2 cut(s) 93, 209
PpsI GAGTC 2 cut(s) 93, 209
Psp1406I AACGTT 1 cut(s) 29
Psp6I CCWGG 5 cut(s) 402, 478, 503, 612, 904
PspGI CCWGG 5 cut(s) 402, 478, 503, 612, 904
PspN4I GGNNCC 2 cut(s) 209, 347
PspPI GGNCC 1 cut(s) 482
PsuI RGATCY 2 cut(s) 17, 463
PvuI CGATCG 1 cut(s) 176
RsaI GTAC 1 cut(s) 915
RsaNI GTAC 1 cut(s) 914
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 4 cut(s) 203, 369, 566, 994
SatI GCNGC 2 cut(s) 243, 292
Sau3AI GATC 4 cut(s) 17, 173, 231, 463
Sau96I GGNCC 1 cut(s) 482
SchI GAGTC 2 cut(s) 94, 209
ScrFI CCNGG 5 cut(s) 404, 480, 505, 614, 906
SetI ASST 8 cut(s) 32, 79, 169, 331, 405, 481, 506, 828
SexAI ACCWGGT 1 cut(s) 478
SfaNI GCATC 3 cut(s) 753, 770, 961
SinI GGWCC 1 cut(s) 482
SsiI CCGC 1 cut(s) 292
SspI AATATT 2 cut(s) 806, 821
SspMI CTAG 1 cut(s) 93
StyD4I CCNGG 5 cut(s) 402, 478, 503, 612, 904
TaaI ACNGT 6 cut(s) 259, 342, 397, 737, 794, 836
TaiI ACGT 2 cut(s) 32, 828
TaqI TCGA 3 cut(s) 172, 234, 959
TaqII GACCGA 1 cut(s) 504
TatI WGTACW 1 cut(s) 913
TauI GCSGC 1 cut(s) 294
Tru1I TTAA 4 cut(s) 203, 369, 566, 994
Tru9I TTAA 4 cut(s) 203, 369, 566, 994
TscAI CASTG 2 cut(s) 347, 952
TseI GCWGC 1 cut(s) 242
TspDTI ATGAA 9 cut(s) 125, 339, 795, 801, 831, 858, 870, 876, 897
TspRI CASTG 2 cut(s) 347, 952
VpaK11BI GGWCC 1 cut(s) 482
XapI RAATTY 2 cut(s) 118, 492
XspI CTAG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.