MD04G1150500.v1.1

Starch binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002509
Physical Location & Seq
Forward (+)
23947969 .. 23951743
3775 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1150500.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGAGGACCCTGTCGAGTCCGAGTTCGAGGGTGGCGGCAGAGAAGAAGTGGGTCGGCGGAGAAAATGTCTTTCTCCACAAGCTTGAACCTTGCTTCCTCAGGCCAAAGAGAAGCAGCGAGAATTTCCGGTTCTTGAATTTGGTGTGTTTTCTGAAACAGAGCAAGCCCATCAGACCAGTCATTTGCCTCTCGTCAAAAACCCAGACAGAATTGGAGGTTGCAGATTCTCAAATGCAGGAAGCATATCATACAAAAACTATCCATGTGAAATTCCAGTTGCAGAAAGAGTGCTCGTTCGGTCAGGAGTTCCTCATTGTGGGGGATGATCCTATGTTCGGTTTGTGGGATCCTGCAAGCGCAATACCGATGAACTGGTCAGATGGGAATGTGTGGACTGTTGAGATGGATATACCAGTTGGGAAATCGATTCAGTTCAAGTTCATACTAAAAGAAAGCACAGGGAATATCTCGTGGCAGCCTGGCCCTGATCGGATTTTCCAGACCTGGGAAACTAAGAATGCGATCACTGTTTGTGAAGACTGGGCAGATGCTGAGCTTCAGAAAATAATAGAGGAAGATCATGTTTCAGATGAAGGCGATGGTTCCAATGTTAATGCAGATATGTTGATTGTGGATGAGAACCTGACACAGCCGAATGATGAACTGGCCTTCAATATAACCAATGAACCTACGATCACCAGTAGTAACACTGATCCGGCAGAGAAGCCATTAGTGGAACCATGGAAGGAACAAATTGGTGCCAGAAATGGTTCTCCTTCACAAGAGAATGTGACCCCTAAATGGCCAAATGCATACGTGGAACCATATGAGGAACAATTTGCTGCAAGAGCTGCAGTGCTTAATGAAGAGAGAGTTATTTTTCCGAGTGAGGATTGTGCAGCCATCTCAAGTAAGGAGTTGTTGGTGGCAGACAACATTTTCGGAAATAATGGTGGAGCTGCAACAGTAATGAACCTTTCAAGCACTCAAATCGGGGGAAGTCTAATTAACTACGAAGAAGGTCCTGTTCTGGTACCTGGGCTGACTCCTTCACCAGCTGTTCCAACTGAAGAAGTGAACAAAGAGGGCATCGACAAACAGATGTCTTTTGATGGTTCGGTTGGAGCCTTTGAAGCTAAGGATCGTAATATGCCAGAGCTAGAAGTGAAGCAAGAACAGTACAGTGACCTACCTCAAGAAGTAACAAATGTAATGATCAACGCTAATGGGGGGAATTTTGACGATGAAATCAAGCAAACGCCTCACCCTGCAAATAAGGAAGAACAGTTCCACTCAGAGCCAGTCAATGATAAGGTCTTGTGCAGTGACCAACCTCAAGTGGAAACAGCTGCCGCAATCAATGATAACGAAGAGAATTCTGCTAAAAAGGAGGAACAGTCCGACTCAGAGCCAATCAACGAGGTCTTTCAGAATGATGTGCAATGGGGTCGTAAGATGCTGCAGAACCTTCTGTCTAACTTCAGATTGCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM_20

Protein Analysis

498

Amino Acids

55.5

Weight (kDa)

4.48

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_20 PF00686 87 - 174 7.1e-21 Starch binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1033
AccB1I GGYRCC 2 cut(s) 758, 1033
AciI CCGC 3 cut(s) 35, 57, 1353
AclWI GGATC 5 cut(s) 320, 341, 354, 707, 1149
AcoI YGGCCR 1 cut(s) 803
AcsI RAATTY 5 cut(s) 121, 136, 269, 1234, 1375
AcuI CTGAAG 3 cut(s) 542, 1089, 1465
AfaI GTAC 2 cut(s) 1035, 1181
AfiI CCNNNNNNNGG 4 cut(s) 316, 335, 505, 801
AgsI TTSAA 6 cut(s) 86, 136, 436, 673, 981, 1133
AjnI CCWGG 3 cut(s) 478, 503, 1036
AloI GAACNNNNNNTCC 4 cut(s) 78, 110, 1271, 1303
AluBI AGCT 8 cut(s) 82, 556, 851, 959, 1058, 1136, 1159, 1349
AluI AGCT 8 cut(s) 82, 556, 851, 959, 1058, 1136, 1159, 1349
Alw21I GWGCWC 1 cut(s) 293
AlwI GGATC 5 cut(s) 320, 341, 354, 707, 1149
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 4 cut(s) 101, 481, 666, 803
ApeKI GCWGC 8 cut(s) 114, 475, 842, 851, 899, 959, 1349, 1459
ApoI RAATTY 5 cut(s) 121, 136, 269, 1234, 1375
ArsI GACNNNNNNTTYG 4 cut(s) 165, 197, 923, 955
Asp700I GAANNNNTTC 1 cut(s) 769
Asp718I GGTACC 1 cut(s) 1033
AspLEI GCGC 1 cut(s) 359
AspS9I GGNCC 3 cut(s) 6, 482, 1022
AsuHPI GGTGA 3 cut(s) 688, 1044, 1256
AvaII GGWCC 2 cut(s) 6, 1022
AxyI CCTNAGG 1 cut(s) 98
BalI TGGCCA 1 cut(s) 805
BamHI GGATCC 1 cut(s) 346
BanI GGYRCC 2 cut(s) 758, 1033
BauI CACGAG 1 cut(s) 469
BbsI GAAGAC 1 cut(s) 543
Bbv12I GWGCWC 1 cut(s) 293
BbvI GCAGC 8 cut(s) 126, 487, 829, 838, 911, 946, 1336, 1446
BccI CCATC 6 cut(s) 176, 374, 397, 593, 911, 1106
BcgI CGANNNNNNTGC 4 cut(s) 973, 1007, 1430, 1464
BciT130I CCWGG 3 cut(s) 480, 505, 1038
BclI TGATCA 1 cut(s) 1215
BfaI CTAG 1 cut(s) 1160
BfmI CTRYAG 3 cut(s) 852, 1460, 1490
BlpI GCTNAGC 1 cut(s) 552
Bme1390I CCNGG 3 cut(s) 480, 505, 1038
Bme18I GGWCC 2 cut(s) 6, 1022
BmgT120I GGNCC 3 cut(s) 6, 482, 1022
BmiI GGNNCC 8 cut(s) 8, 348, 604, 738, 760, 822, 1035, 1126
BmrFI CCNGG 3 cut(s) 480, 505, 1038
BmrI ACTGGG 1 cut(s) 550
BmsI GCATC 3 cut(s) 538, 1098, 1446
BmuI ACTGGG 1 cut(s) 550
BpiI GAAGAC 1 cut(s) 543
Bpu10I CCTNAGC 1 cut(s) 1137
Bpu1102I GCTNAGC 1 cut(s) 552
BpuEI CTTGAG 3 cut(s) 892, 1179, 1320
Bsa29I ATCGAT 1 cut(s) 425
BsaAI YACGTR 1 cut(s) 817
BsaJI CCNNGG 3 cut(s) 504, 740, 1037
BsaWI WCCGGW 1 cut(s) 126
BsaXI ACNNNNNCTCC 4 cut(s) 51, 81, 1382, 1412
Bsc4I CCNNNNNNNGG 4 cut(s) 316, 335, 505, 801
Bse1I ACTGG 8 cut(s) 176, 274, 377, 413, 545, 669, 699, 1301
Bse21I CCTNAGG 1 cut(s) 98
Bse3DI GCAATG 1 cut(s) 1448
BseBI CCWGG 3 cut(s) 480, 505, 1038
BseCI ATCGAT 1 cut(s) 425
BseDI CCNNGG 3 cut(s) 504, 740, 1037
BseGI GGATG 2 cut(s) 328, 640
BseLI CCNNNNNNNGG 4 cut(s) 316, 335, 505, 801
BseMI GCAATG 1 cut(s) 1448
BseMII CTCAG 4 cut(s) 112, 543, 1308, 1419
BseNI ACTGG 8 cut(s) 176, 274, 377, 413, 545, 669, 699, 1301
BseXI GCAGC 8 cut(s) 126, 487, 829, 838, 911, 946, 1336, 1446
BsgI GTGCAG 2 cut(s) 918, 1342
BshFI GGCC 4 cut(s) 103, 483, 668, 805
BshNI GGYRCC 2 cut(s) 758, 1033
BshVI ATCGAT 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 293
BsiSI CCGG 2 cut(s) 127, 716
BslI CCNNNNNNNGG 4 cut(s) 316, 335, 505, 801
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 4 cut(s) 103, 483, 668, 805
Bsp1286I GDGCHC 1 cut(s) 293
Bsp143I GATC 9 cut(s) 325, 346, 487, 522, 577, 693, 712, 1141, 1215
Bsp1720I GCTNAGC 1 cut(s) 552
Bsp19I CCATGG 1 cut(s) 740
BspACI CCGC 3 cut(s) 35, 57, 1353
BspANI GGCC 4 cut(s) 103, 483, 668, 805
BspCNI CTCAG 4 cut(s) 111, 544, 1307, 1418
BspDI ATCGAT 1 cut(s) 425
BspLI GGNNCC 8 cut(s) 8, 348, 604, 738, 760, 822, 1035, 1126
BspMAI CTGCAG 2 cut(s) 856, 1464
BspPI GGATC 5 cut(s) 320, 341, 354, 707, 1149
BspT107I GGYRCC 2 cut(s) 758, 1033
BsrDI GCAATG 1 cut(s) 1448
BsrI ACTGG 8 cut(s) 176, 274, 377, 413, 545, 669, 699, 1301
BssECI CCNNGG 3 cut(s) 504, 740, 1037
BssMI GATC 9 cut(s) 325, 346, 487, 522, 577, 693, 712, 1141, 1215
BssSI CACGAG 1 cut(s) 469
BssT1I CCWWGG 1 cut(s) 740
Bst2BI CACGAG 1 cut(s) 469
Bst2UI CCWGG 3 cut(s) 480, 505, 1038
Bst4CI ACNGT 7 cut(s) 397, 529, 967, 1179, 1184, 1287, 1398
Bst6I CTCTTC 2 cut(s) 861, 1365
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBAI YACGTR 1 cut(s) 817
BstC8I GCNNGC 2 cut(s) 164, 355
BstDEI CTNAG 6 cut(s) 98, 513, 552, 1137, 1294, 1405
BstDSI CCRYGG 1 cut(s) 740
BstF5I GGATG 2 cut(s) 328, 640
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 9 cut(s) 328, 349, 490, 525, 580, 696, 715, 1144, 1218
BstMBI GATC 9 cut(s) 325, 346, 487, 522, 577, 693, 712, 1141, 1215
BstMWI GCNNNNNNNGC 3 cut(s) 848, 851, 1133
BstNI CCWGG 3 cut(s) 480, 505, 1038
BstSCI CCNGG 3 cut(s) 478, 503, 1036
BstSFI CTRYAG 3 cut(s) 852, 1460, 1490
BstV1I GCAGC 8 cut(s) 126, 487, 829, 838, 911, 946, 1336, 1446
BstV2I GAAGAC 1 cut(s) 543
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 425
Bsu36I CCTNAGG 1 cut(s) 98
BsuRI GGCC 4 cut(s) 103, 483, 668, 805
BsuTUI ATCGAT 1 cut(s) 425
BtgI CCRYGG 1 cut(s) 740
BtgZI GCGATG 1 cut(s) 612
BtsCI GGATG 2 cut(s) 328, 640
BtsI GCAGTG 2 cut(s) 861, 1330
BtsIMutI CAGTG 5 cut(s) 525, 708, 861, 1189, 1330
Cac8I GCNNGC 2 cut(s) 164, 355
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 1 cut(s) 359
Cfr13I GGNCC 3 cut(s) 6, 482, 1022
ClaI ATCGAT 1 cut(s) 425
Csp6I GTAC 2 cut(s) 1034, 1180
CviAII CATG 3 cut(s) 263, 581, 741
CviQI GTAC 2 cut(s) 1034, 1180
DdeI CTNAG 6 cut(s) 98, 513, 552, 1137, 1294, 1405
DpnI GATC 9 cut(s) 327, 348, 489, 524, 579, 695, 714, 1143, 1217
DpnII GATC 9 cut(s) 325, 346, 487, 522, 577, 693, 712, 1141, 1215
EaeI YGGCCR 1 cut(s) 803
Eam1104I CTCTTC 2 cut(s) 861, 1365
EarI CTCTTC 2 cut(s) 861, 1365
EciI GGCGGA 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 740
Eco47I GGWCC 2 cut(s) 6, 1022
Eco57I CTGAAG 3 cut(s) 542, 1089, 1465
Eco81I CCTNAGG 1 cut(s) 98
EcoO109I RGGNCCY 2 cut(s) 6, 1022
EcoRI GAATTC 1 cut(s) 1375
EcoRII CCWGG 3 cut(s) 478, 503, 1036
EcoT14I CCWWGG 1 cut(s) 740
EcoT22I ATGCAT 1 cut(s) 814
ErhI CCWWGG 1 cut(s) 740
FaeI CATG 3 cut(s) 266, 584, 744
FatI CATG 3 cut(s) 262, 580, 740
FauNDI CATATG 1 cut(s) 826
FbaI TGATCA 1 cut(s) 1215
FokI GGATG 2 cut(s) 335, 647
FspBI CTAG 1 cut(s) 1160
GlaI GCGC 1 cut(s) 358
HaeIII GGCC 4 cut(s) 103, 483, 668, 805
HapII CCGG 2 cut(s) 127, 716
HhaI GCGC 1 cut(s) 359
Hin1II CATG 3 cut(s) 266, 584, 744
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 5 cut(s) 16, 224, 427, 1045, 1403
HpaII CCGG 2 cut(s) 127, 716
HphI GGTGA 3 cut(s) 688, 1044, 1256
Hpy166II GTNNAC 2 cut(s) 393, 1078
Hpy188III TCNNGA 4 cut(s) 133, 302, 499, 1196
Hpy8I GTNNAC 2 cut(s) 393, 1078
HpyAV CCTTC 7 cut(s) 587, 679, 739, 786, 1013, 1059, 1478
HpyCH4III ACNGT 7 cut(s) 397, 529, 967, 1179, 1184, 1287, 1398
HpyCH4IV ACGT 1 cut(s) 816
HpyF10VI GCNNNNNNNGC 3 cut(s) 848, 851, 1133
HpyF3I CTNAG 6 cut(s) 98, 513, 552, 1137, 1294, 1405
HpySE526I ACGT 1 cut(s) 816
Hsp92II CATG 3 cut(s) 266, 584, 744
HspAI GCGC 1 cut(s) 357
KpnI GGTACC 1 cut(s) 1037
Ksp22I TGATCA 1 cut(s) 1215
Kzo9I GATC 9 cut(s) 325, 346, 487, 522, 577, 693, 712, 1141, 1215
LmnI GCTCC 2 cut(s) 956, 1124
Lsp1109I GCAGC 8 cut(s) 126, 487, 829, 838, 911, 946, 1336, 1446
LweI GCATC 3 cut(s) 538, 1098, 1446
MaeI CTAG 1 cut(s) 1160
MaeII ACGT 1 cut(s) 816
MaeIII GTNAC 5 cut(s) 704, 790, 1184, 1201, 1325
MalI GATC 9 cut(s) 327, 348, 489, 524, 579, 695, 714, 1143, 1217
MboI GATC 9 cut(s) 325, 346, 487, 522, 577, 693, 712, 1141, 1215
MboII GAAGA 8 cut(s) 55, 548, 587, 878, 1028, 1082, 1292, 1382
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 1 cut(s) 293
MlsI TGGCCA 1 cut(s) 805
MluCI AATT 9 cut(s) 121, 136, 209, 269, 753, 836, 1005, 1234, 1375
MluNI TGGCCA 1 cut(s) 805
MlyI GAGTC 3 cut(s) 25, 1039, 1397
MmeI TCCRAC 3 cut(s) 1088, 1102, 1425
Mox20I TGGCCA 1 cut(s) 805
Mph1103I ATGCAT 1 cut(s) 814
MroXI GAANNNNTTC 1 cut(s) 769
MscI TGGCCA 1 cut(s) 805
MseI TTAA 3 cut(s) 612, 861, 1008
Msp20I TGGCCA 1 cut(s) 805
MspA1I CMGCKG 2 cut(s) 1058, 1349
MspI CCGG 2 cut(s) 127, 716
MspR9I CCNGG 3 cut(s) 480, 505, 1038
Mva1269I GAATGC 1 cut(s) 523
MvaI CCWGG 3 cut(s) 480, 505, 1038
MwoI GCNNNNNNNGC 3 cut(s) 848, 851, 1133
NcoI CCATGG 1 cut(s) 740
NdeI CATATG 1 cut(s) 826
NdeII GATC 9 cut(s) 325, 346, 487, 522, 577, 693, 712, 1141, 1215
NlaIII CATG 3 cut(s) 266, 584, 744
NlaIV GGNNCC 8 cut(s) 8, 348, 604, 738, 760, 822, 1035, 1126
NmuCI GTSAC 3 cut(s) 790, 1184, 1325
NsiI ATGCAT 1 cut(s) 814
PctI GAATGC 1 cut(s) 523
PdmI GAANNNNTTC 1 cut(s) 769
PfeI GAWTC 2 cut(s) 224, 427
PflFI GACNNNGTC 1 cut(s) 10
PleI GAGTC 3 cut(s) 24, 1039, 1397
PpsI GAGTC 3 cut(s) 24, 1039, 1397
Ppu21I YACGTR 1 cut(s) 817
PpuMI RGGWCCY 2 cut(s) 6, 1022
Psp5II RGGWCCY 2 cut(s) 6, 1022
Psp6I CCWGG 3 cut(s) 478, 503, 1036
PspGI CCWGG 3 cut(s) 478, 503, 1036
PspN4I GGNNCC 8 cut(s) 8, 348, 604, 738, 760, 822, 1035, 1126
PspPI GGNCC 3 cut(s) 6, 482, 1022
PspPPI RGGWCCY 2 cut(s) 6, 1022
PstI CTGCAG 2 cut(s) 856, 1464
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 1 cut(s) 346
PsyI GACNNNGTC 1 cut(s) 10
PvuII CAGCTG 2 cut(s) 1058, 1349
RsaI GTAC 2 cut(s) 1035, 1181
RsaNI GTAC 2 cut(s) 1034, 1180
SaqAI TTAA 3 cut(s) 612, 861, 1008
Sau3AI GATC 9 cut(s) 325, 346, 487, 522, 577, 693, 712, 1141, 1215
Sau96I GGNCC 3 cut(s) 6, 482, 1022
SchI GAGTC 3 cut(s) 25, 1039, 1397
ScrFI CCNGG 3 cut(s) 480, 505, 1038
SduI GDGCHC 1 cut(s) 293
SfaNI GCATC 3 cut(s) 538, 1098, 1446
SfcI CTRYAG 3 cut(s) 852, 1460, 1490
SinI GGWCC 2 cut(s) 6, 1022
SmlI CTYRAG 3 cut(s) 907, 1194, 1335
SmoI CTYRAG 3 cut(s) 907, 1194, 1335
Sse9I AATT 9 cut(s) 121, 136, 209, 269, 753, 836, 1005, 1234, 1375
SsiI CCGC 3 cut(s) 35, 57, 1353
SspMI CTAG 1 cut(s) 1160
StyD4I CCNGG 3 cut(s) 478, 503, 1036
StyI CCWWGG 1 cut(s) 740
TaaI ACNGT 7 cut(s) 397, 529, 967, 1179, 1184, 1287, 1398
TaiI ACGT 1 cut(s) 819
TaqI TCGA 4 cut(s) 14, 26, 425, 1092
TaqII GACCGA 1 cut(s) 287
TasI AATT 9 cut(s) 121, 136, 209, 269, 753, 836, 1005, 1234, 1375
TatI WGTACW 1 cut(s) 1179
TauI GCSGC 2 cut(s) 38, 1355
TfiI GAWTC 2 cut(s) 224, 427
Tru1I TTAA 3 cut(s) 612, 861, 1008
Tru9I TTAA 3 cut(s) 612, 861, 1008
TscAI CASTG 5 cut(s) 532, 715, 861, 1189, 1330
TseFI GTSAC 3 cut(s) 790, 1184, 1325
TseI GCWGC 8 cut(s) 114, 475, 842, 851, 899, 959, 1349, 1459
Tsp45I GTSAC 3 cut(s) 790, 1184, 1325
TspDTI ATGAA 8 cut(s) 383, 430, 606, 675, 699, 879, 986, 1260
TspRI CASTG 5 cut(s) 532, 715, 861, 1189, 1330
Tth111I GACNNNGTC 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 6, 1022
XapI RAATTY 5 cut(s) 121, 136, 269, 1234, 1375
XmnI GAANNNNTTC 1 cut(s) 769
XspI CTAG 1 cut(s) 1160
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.