Rh3CG128100

Starch binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0002509
Physical Location & Seq
Reverse (-)
10428092 .. 10431020
2929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG128100.1

Sequence Viewer

Length: 1512 bp
ATGAAAACGTTGGCGAGTCCGAGTTCGAGGGTGCTCTTGGAAATTTACAGGGACAGAGAGGCTGGCTTGGTGAACAGGGTTGAAGAAGCTTCGTGCTTTTTCAGGCCTCACAAGAAAAATGGAGACTTTCGGTTCTTGAATTTGGTGGCGTTTCTGCAACAGAGAAAGCCCATCAAACCTGTTGTTTGTCTTTCATCGAAAACCCAGGCAGAATTGGAGACTACAGATGCTCAGATTCAAGAAACATATCCAACAAAAACTATGCATGTCAAGTTCCAGTTGCAGAAAGAGTGCTCCTTTGGTCAGCATTTCCTCATAGTAGGGGATGATCCTATGTTGGGTTTATGGGACCCTGAGAGAGCAATACAGATGGACTGGTCTGATGGGAATGTGTGGTCTGTTGAGCTGGATATACCTGTTGGGAAATCAATCCAGTTCAAGTTCATACTAAAAGGAAGCGCAGGGAATATCTTGTGGCAACCTGGCCCAGATCGAATTTTTCAAACCTGGGAAACTGAGAACACAATCACTGTTTCTGAAGATTGGGGAGATGCTGAACTTCAGAAAATAACAGAAGAAGCACAAGTTTATAATCAAATTGAGGATTCTAATATCAACTCAGATATATTGATTCCAGAGGATGACAACTTGACTTTTCCAGAAGAGGAGCCAGTGTTTAATATAAACATGGAGTCGGCCAATGTTGATAGTTACACTGATCCAGCACAGATACCGTTAGTGGAATCATACCAGGAACAAATTGTTGCTGATAACATATCTGTCTCACAAGAGAAGCCCAAAACTTTTGTTGCTGAGAATATAACACCAAAATGGCCAAAGCAAATGATGGAACCATACGAGGAACAAATGGTTGCAAGAACTAATGCCCCAAGTGAGGATTCAGCAGCCATCCCAAACGAGATGCTGGTGGCACAGAACATTTTTGGAAATAATGGCAGAGCTGCAACAGCAGGGAACCTCGCAAGCTCAAACACTGAGGAAAGCCTAATGAACTATGAGGAAGAAGGACCTGTTCTGGTACCTGGCTTGAGTCGTGCACCAGCTGTTCCAACTGAAGAAGCAAACAAAGAGGAAGTTGAGAAACCAATGACCTTTGATGGCTCGGTTGGAGCTTATGAAGCTAAGGAACTGAATATGCCAGAGCTAGAAGAGAAGCAAGAACCATACAGGGAATCACCTCAAGCAGAAACAATTGAGATGTTCAATGATAATATAGAGAAATTTGATGAAGAACTCAAGCAAAAGCCTCAACCAGCTGAAAGGGAAAATCAGTCCAAGCCTGACCTACCCTATGGCTCACCTCAAACAGAAACAACTGACATGTTCAACAATAATGAAGTGAGTTTTGATGGTGGACTCGAGCAAAAGCATCAAACTGCTGAAACTGCAGAACAGTCTGGTTCAGAGCAAATCCATGATGTCTGGCACAGTGACATGCAATGGGGTCGTAAAATACTGCAGAAACTTCTGATTACTTTCAGATTGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM_20

Protein Analysis

503

Amino Acids

57.08

Weight (kDa)

4.44

Isoelectric Point (pI)

59.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_20 PF00686 88 - 176 5e-21 Starch binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
Acc65I GGTACC 1 cut(s) 1039
AccB1I GGYRCC 1 cut(s) 1039
AclI AACGTT 1 cut(s) 8
AclWI GGATC 2 cut(s) 323, 713
AcoI YGGCCR 2 cut(s) 696, 833
AcsI RAATTY 4 cut(s) 42, 139, 495, 1241
AcuI CTGAAG 3 cut(s) 545, 558, 1095
AfaI GTAC 1 cut(s) 1041
AfiI CCNNNNNNNGG 2 cut(s) 338, 895
AflIII ACRYGT 1 cut(s) 1341
AgsI TTSAA 7 cut(s) 83, 139, 239, 439, 503, 1225, 1348
AjnI CCWGG 5 cut(s) 204, 481, 506, 750, 1042
AjuI GAANNNNNNNTTGG 2 cut(s) 883, 915
AluBI AGCT 9 cut(s) 89, 406, 962, 987, 1064, 1133, 1142, 1165, 1277
AluI AGCT 9 cut(s) 89, 406, 962, 987, 1064, 1133, 1142, 1165, 1277
Alw21I GWGCWC 3 cut(s) 36, 296, 1060
Alw26I GTCTC 3 cut(s) 117, 212, 787
Alw44I GTGCAC 1 cut(s) 1056
AlwI GGATC 2 cut(s) 323, 713
Ama87I CYCGRG 1 cut(s) 1379
AoxI GGCC 4 cut(s) 104, 484, 696, 833
ApaLI GTGCAC 1 cut(s) 1056
ApeKI GCWGC 2 cut(s) 905, 962
ApoI RAATTY 4 cut(s) 42, 139, 495, 1241
Asp718I GGTACC 1 cut(s) 1039
AspLEI GCGC 1 cut(s) 461
AspS9I GGNCC 3 cut(s) 349, 485, 1028
AsuHPI GGTGA 3 cut(s) 82, 1188, 1311
AvaI CYCGRG 1 cut(s) 1379
AvaII GGWCC 2 cut(s) 349, 1028
BaeGI GKGCMC 1 cut(s) 1060
BalI TGGCCA 1 cut(s) 835
BanI GGYRCC 1 cut(s) 1039
Bbv12I GWGCWC 3 cut(s) 36, 296, 1060
BbvI GCAGC 2 cut(s) 917, 949
BccI CCATC 7 cut(s) 179, 364, 377, 841, 917, 1112, 1364
BcgI CGANNNNNNTGC 2 cut(s) 1448, 1482
BciT130I CCWGG 5 cut(s) 206, 483, 508, 752, 1044
BcoDI GTCTC 3 cut(s) 117, 212, 787
BfaI CTAG 1 cut(s) 1166
BfmI CTRYAG 3 cut(s) 222, 1407, 1478
BisI GCNGC 2 cut(s) 906, 963
BlsI GCNGC 2 cut(s) 907, 964
Bme1390I CCNGG 5 cut(s) 206, 483, 508, 752, 1044
Bme18I GGWCC 2 cut(s) 349, 1028
BmeT110I CYCGRG 1 cut(s) 1379
BmgT120I GGNCC 3 cut(s) 349, 485, 1028
BmiI GGNNCC 6 cut(s) 350, 351, 669, 852, 977, 1041
BmrFI CCNGG 5 cut(s) 206, 483, 508, 752, 1044
BmsI GCATC 4 cut(s) 217, 541, 912, 1399
Bpu10I CCTNAGC 1 cut(s) 1143
BpuEI CTTGAG 3 cut(s) 1069, 1185, 1241
BsaJI CCNNGG 2 cut(s) 204, 507
BsaXI ACNNNNNCTCC 2 cut(s) 659, 689
Bsc4I CCNNNNNNNGG 2 cut(s) 338, 895
Bse1I ACTGG 4 cut(s) 277, 380, 433, 671
Bse3DI GCAATG 1 cut(s) 1466
BseBI CCWGG 5 cut(s) 206, 483, 508, 752, 1044
BseDI CCNNGG 2 cut(s) 204, 507
BseGI GGATG 3 cut(s) 331, 646, 909
BseLI CCNNNNNNNGG 2 cut(s) 338, 895
BseMI GCAATG 1 cut(s) 1466
BseMII CTCAG 6 cut(s) 245, 345, 507, 633, 804, 987
BseNI ACTGG 4 cut(s) 277, 380, 433, 671
BseRI GAGGAG 1 cut(s) 680
BseSI GKGCMC 1 cut(s) 1060
BseXI GCAGC 2 cut(s) 917, 949
BshFI GGCC 4 cut(s) 106, 486, 698, 835
BshNI GGYRCC 1 cut(s) 1039
BsiHKAI GWGCWC 3 cut(s) 36, 296, 1060
BsiHKCI CYCGRG 1 cut(s) 1379
BslFI GGGAC 2 cut(s) 65, 362
BslI CCNNNNNNNGG 2 cut(s) 338, 895
BsmAI GTCTC 3 cut(s) 117, 212, 787
BsmFI GGGAC 2 cut(s) 65, 362
BsnI GGCC 4 cut(s) 106, 486, 698, 835
BsoBI CYCGRG 1 cut(s) 1379
Bsp1286I GDGCHC 3 cut(s) 36, 296, 1060
Bsp143I GATC 3 cut(s) 328, 490, 718
BspANI GGCC 4 cut(s) 106, 486, 698, 835
BspCNI CTCAG 6 cut(s) 244, 346, 508, 632, 805, 988
BspLI GGNNCC 6 cut(s) 350, 351, 669, 852, 977, 1041
BspMAI CTGCAG 2 cut(s) 1411, 1482
BspPI GGATC 2 cut(s) 323, 713
BspT107I GGYRCC 1 cut(s) 1039
BsrDI GCAATG 1 cut(s) 1466
BsrI ACTGG 4 cut(s) 277, 380, 433, 671
BssECI CCNNGG 2 cut(s) 204, 507
BssMI GATC 3 cut(s) 328, 490, 718
Bst2UI CCWGG 5 cut(s) 206, 483, 508, 752, 1044
Bst4CI ACNGT 4 cut(s) 532, 735, 1416, 1451
Bst6I CTCTTC 2 cut(s) 657, 1164
BstC8I GCNNGC 2 cut(s) 64, 985
BstDEI CTNAG 8 cut(s) 231, 354, 516, 619, 813, 996, 1143, 1509
BstF5I GGATG 3 cut(s) 331, 646, 909
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 3 cut(s) 331, 493, 721
BstMAI GTCTC 3 cut(s) 117, 212, 787
BstMBI GATC 3 cut(s) 328, 490, 718
BstMWI GCNNNNNNNGC 3 cut(s) 968, 1139, 1406
BstNI CCWGG 5 cut(s) 206, 483, 508, 752, 1044
BstNSI RCATGY 3 cut(s) 269, 1345, 1459
BstSCI CCNGG 5 cut(s) 204, 481, 506, 750, 1042
BstSFI CTRYAG 3 cut(s) 222, 1407, 1478
BstSLI GKGCMC 1 cut(s) 1060
BstV1I GCAGC 2 cut(s) 917, 949
BsuRI GGCC 4 cut(s) 106, 486, 698, 835
BtsCI GGATG 3 cut(s) 331, 646, 909
BtsIMutI CAGTG 5 cut(s) 528, 678, 714, 993, 1456
Cac8I GCNNGC 2 cut(s) 64, 985
CfoI GCGC 1 cut(s) 461
Cfr13I GGNCC 3 cut(s) 349, 485, 1028
Csp6I GTAC 1 cut(s) 1040
CviAII CATG 5 cut(s) 266, 688, 1342, 1436, 1456
CviQI GTAC 1 cut(s) 1040
DdeI CTNAG 8 cut(s) 231, 354, 516, 619, 813, 996, 1143, 1509
DpnI GATC 3 cut(s) 330, 492, 720
DpnII GATC 3 cut(s) 328, 490, 718
EaeI YGGCCR 2 cut(s) 696, 833
Eam1104I CTCTTC 2 cut(s) 657, 1164
EarI CTCTTC 2 cut(s) 657, 1164
Eco147I AGGCCT 1 cut(s) 106
Eco47I GGWCC 2 cut(s) 349, 1028
Eco57I CTGAAG 3 cut(s) 545, 558, 1095
Eco88I CYCGRG 1 cut(s) 1379
EcoO109I RGGNCCY 2 cut(s) 349, 1028
EcoRII CCWGG 5 cut(s) 204, 481, 506, 750, 1042
EcoT22I ATGCAT 1 cut(s) 267
FaeI CATG 5 cut(s) 269, 691, 1345, 1439, 1459
FaqI GGGAC 2 cut(s) 65, 362
FatI CATG 5 cut(s) 265, 687, 1341, 1435, 1455
Fnu4HI GCNGC 2 cut(s) 906, 963
FokI GGATG 3 cut(s) 338, 653, 896
Fsp4HI GCNGC 2 cut(s) 906, 963
FspBI CTAG 1 cut(s) 1166
GlaI GCGC 1 cut(s) 460
GluI GCNGC 2 cut(s) 906, 963
HaeIII GGCC 4 cut(s) 106, 486, 698, 835
HhaI GCGC 1 cut(s) 461
Hin1II CATG 5 cut(s) 269, 691, 1345, 1439, 1459
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HindIII AAGCTT 1 cut(s) 87
HphI GGTGA 3 cut(s) 82, 1188, 1311
Hpy166II GTNNAC 3 cut(s) 73, 1058, 1376
Hpy188I TCNGA 9 cut(s) 21, 234, 382, 538, 564, 622, 1426, 1491, 1502
Hpy188III TCNNGA 4 cut(s) 136, 239, 635, 659
Hpy8I GTNNAC 3 cut(s) 73, 1058, 1376
HpyAV CCTTC 1 cut(s) 1019
HpyCH4III ACNGT 4 cut(s) 532, 735, 1416, 1451
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 9 cut(s) 157, 265, 283, 875, 965, 1058, 1409, 1459, 1480
HpyF10VI GCNNNNNNNGC 3 cut(s) 968, 1139, 1406
HpyF3I CTNAG 8 cut(s) 231, 354, 516, 619, 813, 996, 1143, 1509
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 5 cut(s) 269, 691, 1345, 1439, 1459
HspAI GCGC 1 cut(s) 459
KflI GGGWCCC 1 cut(s) 349
KpnI GGTACC 1 cut(s) 1043
Kzo9I GATC 3 cut(s) 328, 490, 718
LmnI GCTCC 3 cut(s) 299, 667, 1130
Lsp1109I GCAGC 2 cut(s) 917, 949
LweI GCATC 4 cut(s) 217, 541, 912, 1399
MaeI CTAG 1 cut(s) 1166
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 2 cut(s) 710, 1451
MalI GATC 3 cut(s) 330, 492, 720
MboI GATC 3 cut(s) 328, 490, 718
MboII GAAGA 8 cut(s) 95, 551, 587, 674, 1034, 1088, 1181, 1262
MfeI CAATTG 1 cut(s) 1212
MhlI GDGCHC 3 cut(s) 36, 296, 1060
MlsI TGGCCA 1 cut(s) 835
MluCI AATT 8 cut(s) 42, 139, 212, 495, 597, 759, 1212, 1241
MluNI TGGCCA 1 cut(s) 835
MlyI GAGTC 4 cut(s) 25, 701, 1060, 1371
MmeI TCCRAC 3 cut(s) 275, 1094, 1108
Mox20I TGGCCA 1 cut(s) 835
Mph1103I ATGCAT 1 cut(s) 267
MscI TGGCCA 1 cut(s) 835
MseI TTAA 1 cut(s) 678
MslI CAYNNNNRTG 1 cut(s) 829
Msp20I TGGCCA 1 cut(s) 835
MspA1I CMGCKG 2 cut(s) 1064, 1277
MspR9I CCNGG 5 cut(s) 206, 483, 508, 752, 1044
MunI CAATTG 1 cut(s) 1212
MvaI CCWGG 5 cut(s) 206, 483, 508, 752, 1044
MwoI GCNNNNNNNGC 3 cut(s) 968, 1139, 1406
NdeII GATC 3 cut(s) 328, 490, 718
NlaIII CATG 5 cut(s) 269, 691, 1345, 1439, 1459
NlaIV GGNNCC 6 cut(s) 350, 351, 669, 852, 977, 1041
NmuCI GTSAC 1 cut(s) 1451
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 3 cut(s) 269, 1345, 1459
PaeR7I CTCGAG 1 cut(s) 1379
PceI AGGCCT 1 cut(s) 106
PciI ACATGT 1 cut(s) 1341
PfeI GAWTC 6 cut(s) 235, 605, 631, 743, 899, 1193
PkrI GCNGC 2 cut(s) 907, 964
PleI GAGTC 4 cut(s) 24, 700, 1059, 1371
PpsI GAGTC 4 cut(s) 24, 700, 1059, 1371
PpuMI RGGWCCY 2 cut(s) 349, 1028
PscI ACATGT 1 cut(s) 1341
PsiI TTATAA 1 cut(s) 591
Psp1406I AACGTT 1 cut(s) 8
Psp5II RGGWCCY 2 cut(s) 349, 1028
Psp6I CCWGG 5 cut(s) 204, 481, 506, 750, 1042
PspGI CCWGG 5 cut(s) 204, 481, 506, 750, 1042
PspN4I GGNNCC 6 cut(s) 350, 351, 669, 852, 977, 1041
PspPI GGNCC 3 cut(s) 349, 485, 1028
PspPPI RGGWCCY 2 cut(s) 349, 1028
PspXI VCTCGAGB 1 cut(s) 1379
PstI CTGCAG 2 cut(s) 1411, 1482
PvuII CAGCTG 2 cut(s) 1064, 1277
RsaI GTAC 1 cut(s) 1041
RsaNI GTAC 1 cut(s) 1040
RseI CAYNNNNRTG 1 cut(s) 829
SaqAI TTAA 1 cut(s) 678
SatI GCNGC 2 cut(s) 906, 963
Sau3AI GATC 3 cut(s) 328, 490, 718
Sau96I GGNCC 3 cut(s) 349, 485, 1028
SchI GAGTC 4 cut(s) 25, 701, 1060, 1371
ScrFI CCNGG 5 cut(s) 206, 483, 508, 752, 1044
SduI GDGCHC 3 cut(s) 36, 296, 1060
SfaNI GCATC 4 cut(s) 217, 541, 912, 1399
SfcI CTRYAG 3 cut(s) 222, 1407, 1478
Sfr274I CTCGAG 1 cut(s) 1379
SinI GGWCC 2 cut(s) 349, 1028
SlaI CTCGAG 1 cut(s) 1379
SmiMI CAYNNNNRTG 1 cut(s) 829
SmlI CTYRAG 4 cut(s) 1048, 1200, 1256, 1379
SmoI CTYRAG 4 cut(s) 1048, 1200, 1256, 1379
Sse9I AATT 8 cut(s) 42, 139, 212, 495, 597, 759, 1212, 1241
SseBI AGGCCT 1 cut(s) 106
SspMI CTAG 1 cut(s) 1166
StuI AGGCCT 1 cut(s) 106
StyD4I CCNGG 5 cut(s) 204, 481, 506, 750, 1042
TaaI ACNGT 4 cut(s) 532, 735, 1416, 1451
TaiI ACGT 1 cut(s) 11
TaqI TCGA 4 cut(s) 26, 197, 493, 1380
TasI AATT 8 cut(s) 42, 139, 212, 495, 597, 759, 1212, 1241
TfiI GAWTC 6 cut(s) 235, 605, 631, 743, 899, 1193
Tru1I TTAA 1 cut(s) 678
Tru9I TTAA 1 cut(s) 678
TscAI CASTG 5 cut(s) 535, 678, 721, 1000, 1456
TseFI GTSAC 1 cut(s) 1451
TseI GCWGC 2 cut(s) 905, 962
Tsp45I GTSAC 1 cut(s) 1451
TspDTI ATGAA 7 cut(s) 17, 183, 433, 1025, 1152, 1263, 1371
TspRI CASTG 5 cut(s) 535, 678, 721, 1000, 1456
VneI GTGCAC 1 cut(s) 1056
VpaK11BI GGWCC 2 cut(s) 349, 1028
XapI RAATTY 4 cut(s) 42, 139, 495, 1241
XceI RCATGY 3 cut(s) 269, 1345, 1459
XhoI CTCGAG 1 cut(s) 1379
XspI CTAG 1 cut(s) 1166
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.