Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002509

Physical Location & Seq

Genomic Coordinates Forward (+)

24596875 .. 24601323

Gene Structure 4449 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1164600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2172 bp

ATGAGGACCCTGTCGAGTCCGAGTTCGAGGGTGGCGGTAGAAAAGAAGTGGGTGGGCAGAGAAAATGTCTTTCCCCGCGAGCTGGAACCTTGTTTCCTCAGGCCAAGGAGAAGTGGGAACCTTCGGTTCTTGAATTTGGTGTGTTTTCTGAAACAGAGCAAGCCCGTGATCAGACCGATCATTTGCCTCTCATCAAAAACTCAGACAGAATTGGAGATTGCAGATTCTCAAATTCAGGAAACACATCATACAAAAGCTATTCATGTGAAATTCCAGTTGCAGAAGGAGTGCTCGTTCGGGCAGGAGTTCCTCATTGTAGGGGATGATCCTATGTTCGGTTTGTGGGATCCTGCAAGCGCAATACCGATGATCTGGTCAGATGGGAATGTGTGGACTGTTGAGCTGGATATACCGGTTGGGAAATCGATTCAGTTCAAGTTCATACTAAAAGAAAGCACAGGGAATATCTCATGGCAGCCTGGCCCTGATCGGATTTTCCAGACCTGGGAAACTAGGAACAGGATCACTGTTTCTGAAGATTGGGCAGATGCTGAGCTTCAGAAAATAATAGAGGAAGATCGGGTTTCAAATCAAAGCGATGGTTCAAATGTTAACGCAGATATGTTGATTATGGATGAGAACCTGACACAGCTGAATGGTGAACTGGCCTTCAATACAACCAATGATCCTAAAATCACCAATAGTAACACTGATTCGGCAGAGAACCCATCAGTAGAACCAAGGAAGGAACAAATTGGTGCTGATAATGGTTCTCCTTCACAAGAGAATATGACCCCTAAATGGCCAAAGGCATACGTGGAACCATACAAGGAACGAGTTGCTGCAAGAACTGCAGTGCTTAATGAAGAGAGAGTTATTTTTCCGAGCAAGGATTCTGCAGCCATCTCAAGTAAGGAGTTGTTGGTGGCAGACAACATTTTCGGAAATAATGGTAGAGCTGCAACAGTAATGAACCTTACGAGCACTCACATCGAGGGAAGCCTAGTTAACCATGAAGAAGATCCTGTTCTGGTACCTGGGCTGACTCCTTCACCAGCTGTTCCAAATGAAGAAGTGAACAAAGAGGGAATCGAGAAACAGATGTCTTTTGATGGCTCGGTTGGAGCCTTTGAAGCTAAGGATCGTAATATGCCAGAGAGCAAGCCCGTGATCAGACCGATCATTTGCCTCTCATCAAAAACCCAGACAGAATTGGAGATTGCAGATTCTCAAATTCAGGAAACATATCATACAAAAACTATTCGTGTGAGATTCCAGTTGCAGAAGGAGTGCTCGTTCGGGCAGGAGTTCCTCATTGTAGGGGATGATCCTATGTTCGGTTTGTGGGATCCTGCAAGCGCAATACCTATGAACTGGTCAGATGGGAATGTATGGACTGTTGAGCTGGATATATCAGTTGGGAAATCGATTCAGTTCAAGTTCATACTACAAGAAAGCACAGGGAATATCTCATGGCAGCCTGGCCCTGATCGGATTTTCCAGACCTGGGAAACTATGAACAGGATCACTGTTTCCGAAGATTGGGCAGATGCTGAGCTTCAGAAAATAATAGAGGAAGATCAGGTTTCAAATCAAAGCGATGGTTCAAATGTTAACGCAGATATGTTGAATATGGATGAGAACCTGACACAGCCGAATGGTGAACTGGCCTTCAATACAACCAATGAACCGAAAATCACCAATAGTAACACTGATTCGGCAGAGAAACCATCAGTAGAACCAAGGAAGGAACAAATTGGTGCTGATAATGGTTCTCCTTCACAAGAGAATGTGAACCCTAAATGGCCAAAGGCATATGTGGAATCATACAAGGAACGAGTTGCCACAAGAACTGCAGTGCTTAATGAAGAGAGAGTTATTTTTCCAAGCGAGGATTCTACAGCCATCTCAAGTAAGGAGTTGTTGGTGGCAGACAACATCTGCGGAAATAATGGTAGAGCTGCAACAGTAATGAACCTTTCAAGCACTCACGTCGAGGGAAGCCTAGTTAACCACGAAGAAGGTCCTGTTCTGGTACCTGGGCTGACTCCTTCACCAGCTGTTCCAAATGAAGAAGTGAACAAAGAGGGAATCGAGAAACAGATGTCTTTTGATGGCTCGGTTGGAGCATTTGAAGCTAAGGATCGTAATATGCCAGAGGTAACTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_20

Protein Analysis

724

Amino Acids

80.47

Weight (kDa)

4.68

Isoelectric Point (pI)

45.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	87 - 174	8.4e-20	Starch binding domain
CBM_20	PF00686	421 - 508	7.2e-20	Starch binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002509)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01260 AT5G01260 AT5G01260
fragaria_vesca	FvH4_3g38430 FvH4_6g11650 FvH4_6g11650 FvH4_6g11650
malus_domestica	MD04G1150500.v1.1 MD12G1164600.v1.1
prunus_persica	Prupe.6G058700_v2.0.a1 Prupe.6G275300_v2.0.a1 Prupe.6G275300_v2.0.a1
pyrus_communis	pycom04g13610 pycom04g13620 pycom11g06380 pycom12g15750
rosa_chinensis	RchiOBHm_Chr3g0463311
rosa_laevigata	RLG00000024791 RLG00000036036
rosa_multiflora	Rmu_co8319585.1_g000001 Rmu_sc0000853.1_g000034 Rmu_sc0000923.1_g000002 Rmu_sc0040359.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011670 Rroxscaffold_6G00416670
rosa_rugosa	Rorug03G0063800 Rorug03G0063900 Rorug03G0064000 Rorug03G0064100 Rorug05G0396200
rosa_samantha	Rh3AG121700 Rh3BG126200 Rh3CG128100 Rh3DG127300 Rh5AG452900 Rh5BG471900 Rh5CG494300 Rh5DG484400
rosa_wichuraiana	Rw0G006070 Rw3G010340 Rw5G042290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	1033, 2035
AccB1I	GGYRCC	2 cut(s)	1033, 2035
AccII	CGCG	1 cut(s)	78
AciI	CCGC	3 cut(s)	35, 76, 1944
AcoI	YGGCCR	2 cut(s)	803, 1805
AcsI	RAATTY	4 cut(s)	133, 231, 269, 1233
AcuI	CTGAAG	3 cut(s)	542, 555, 1544
AfaI	GTAC	2 cut(s)	1035, 2037
AfiI	CCNNNNNNNGG	7 cut(s)	82, 335, 505, 801, 1337, 1507, 1542
AgeI	ACCGGT	1 cut(s)	412
AjiI	CACGTC	1 cut(s)	1993
AjnI	CCWGG	6 cut(s)	478, 503, 1036, 1480, 1505, 2038
AjuI	GAANNNNNNNTTGG	2 cut(s)	1879, 1911
AloI	GAACNNNNNNTCC	6 cut(s)	78, 110, 278, 310, 1280, 1312
Alw21I	GWGCWC	3 cut(s)	293, 986, 1295
AlwNI	CAGNNNCTG	2 cut(s)	551, 1553
AoxI	GGCC	7 cut(s)	101, 481, 666, 803, 1483, 1668, 1805
ApeKI	GCWGC	6 cut(s)	475, 842, 899, 959, 1477, 1961
ApoI	RAATTY	4 cut(s)	133, 231, 269, 1233
ArsI	GACNNNNNNTTYG	6 cut(s)	165, 197, 923, 955, 1167, 1199
AsiGI	ACCGGT	1 cut(s)	412
Asp718I	GGTACC	2 cut(s)	1033, 2035
AspLEI	GCGC	2 cut(s)	359, 1361
AspS9I	GGNCC	4 cut(s)	6, 482, 1484, 2024
AsuHPI	GGTGA	6 cut(s)	671, 688, 1044, 1673, 1690, 2046
AvaII	GGWCC	2 cut(s)	6, 2024
AxyI	CCTNAGG	1 cut(s)	98
BalI	TGGCCA	2 cut(s)	805, 1807
BamHI	GGATCC	2 cut(s)	346, 1348
BanI	GGYRCC	2 cut(s)	1033, 2035
Bbv12I	GWGCWC	3 cut(s)	293, 986, 1295
BbvI	GCAGC	6 cut(s)	487, 829, 911, 946, 1489, 1948
BcgI	CGANNNNNNTGC	4 cut(s)	973, 1007, 1975, 2009
BciT130I	CCWGG	6 cut(s)	480, 505, 1038, 1482, 1507, 2040
BclI	TGATCA	2 cut(s)	168, 1170
BfaI	CTAG	3 cut(s)	513, 1004, 2006
BfmI	CTRYAG	4 cut(s)	852, 897, 1854, 1899
BisI	GCNGC	6 cut(s)	476, 843, 900, 960, 1478, 1962
BlpI	GCTNAGC	2 cut(s)	552, 1554
BlsI	GCNGC	6 cut(s)	477, 844, 901, 961, 1479, 1963
Bme1390I	CCNGG	6 cut(s)	480, 505, 1038, 1482, 1507, 2040
Bme18I	GGWCC	2 cut(s)	6, 2024
BmgBI	CACGTC	1 cut(s)	1993
BmgT120I	GGNCC	4 cut(s)	6, 482, 1484, 2024
BmiI	GGNNCC	9 cut(s)	8, 87, 119, 348, 822, 1035, 1126, 1350, 2037
BmrFI	CCNGG	6 cut(s)	480, 505, 1038, 1482, 1507, 2040
BmsI	GCATC	2 cut(s)	538, 1540
Bpu10I	CCTNAGC	2 cut(s)	1137, 2139
Bpu1102I	GCTNAGC	2 cut(s)	552, 1554
BpuEI	CTTGAG	2 cut(s)	892, 1894
Bsa29I	ATCGAT	2 cut(s)	425, 1427
BsaAI	YACGTR	1 cut(s)	817
BsaJI	CCNNGG	7 cut(s)	104, 504, 740, 1037, 1506, 1742, 2039
BsaWI	WCCGGW	1 cut(s)	412
BsaXI	ACNNNNNCTCC	4 cut(s)	278, 308, 1280, 1310
Bsc4I	CCNNNNNNNGG	7 cut(s)	82, 335, 505, 801, 1337, 1507, 1542
Bse118I	RCCGGY	1 cut(s)	412
Bse1I	ACTGG	5 cut(s)	274, 669, 1276, 1379, 1671
Bse21I	CCTNAGG	1 cut(s)	98
BseBI	CCWGG	6 cut(s)	480, 505, 1038, 1482, 1507, 2040
BseCI	ATCGAT	2 cut(s)	425, 1427
BseDI	CCNNGG	7 cut(s)	104, 504, 740, 1037, 1506, 1742, 2039
BseGI	GGATG	4 cut(s)	328, 640, 1330, 1642
BseLI	CCNNNNNNNGG	7 cut(s)	82, 335, 505, 801, 1337, 1507, 1542
BseMII	CTCAG	4 cut(s)	112, 215, 543, 1545
BseNI	ACTGG	5 cut(s)	274, 669, 1276, 1379, 1671
BseXI	GCAGC	6 cut(s)	487, 829, 911, 946, 1489, 1948
Bsh1236I	CGCG	1 cut(s)	78
BshFI	GGCC	7 cut(s)	103, 483, 668, 805, 1485, 1670, 1807
BshNI	GGYRCC	2 cut(s)	1033, 2035
BshTI	ACCGGT	1 cut(s)	412
BshVI	ATCGAT	2 cut(s)	425, 1427
BsiHKAI	GWGCWC	3 cut(s)	293, 986, 1295
BsiSI	CCGG	1 cut(s)	413
BslI	CCNNNNNNNGG	7 cut(s)	82, 335, 505, 801, 1337, 1507, 1542
BsnI	GGCC	7 cut(s)	103, 483, 668, 805, 1485, 1670, 1807
Bsp1286I	GDGCHC	3 cut(s)	293, 986, 1295
Bsp1720I	GCTNAGC	2 cut(s)	552, 1554
BspACI	CCGC	3 cut(s)	35, 76, 1944
BspANI	GGCC	7 cut(s)	103, 483, 668, 805, 1485, 1670, 1807
BspCNI	CTCAG	4 cut(s)	111, 214, 544, 1546
BspDI	ATCGAT	2 cut(s)	425, 1427
BspFNI	CGCG	1 cut(s)	78
BspLI	GGNNCC	9 cut(s)	8, 87, 119, 348, 822, 1035, 1126, 1350, 2037
BspMAI	CTGCAG	3 cut(s)	856, 901, 1858
BspT107I	GGYRCC	2 cut(s)	1033, 2035
BsrFI	RCCGGY	1 cut(s)	412
BsrI	ACTGG	5 cut(s)	274, 669, 1276, 1379, 1671
BssAI	RCCGGY	1 cut(s)	412
BssECI	CCNNGG	7 cut(s)	104, 504, 740, 1037, 1506, 1742, 2039
BssT1I	CCWWGG	3 cut(s)	104, 740, 1742
Bst2UI	CCWGG	6 cut(s)	480, 505, 1038, 1482, 1507, 2040
Bst4CI	ACNGT	6 cut(s)	397, 529, 967, 1399, 1531, 1969
Bst6I	CTCTTC	2 cut(s)	861, 1863
BstAPI	GCANNNNNTGC	1 cut(s)	851
BstBAI	YACGTR	1 cut(s)	817
BstC8I	GCNNGC	5 cut(s)	80, 161, 355, 1163, 1357
BstDEI	CTNAG	6 cut(s)	98, 201, 552, 1137, 1554, 2139
BstF5I	GGATG	4 cut(s)	328, 640, 1330, 1642
BstFNI	CGCG	1 cut(s)	78
BstHHI	GCGC	2 cut(s)	359, 1361
BstMWI	GCNNNNNNNGC	3 cut(s)	851, 1133, 2135
BstNI	CCWGG	6 cut(s)	480, 505, 1038, 1482, 1507, 2040
BstSCI	CCNGG	6 cut(s)	478, 503, 1036, 1480, 1505, 2038
BstSFI	CTRYAG	4 cut(s)	852, 897, 1854, 1899
BstUI	CGCG	1 cut(s)	78
BstV1I	GCAGC	6 cut(s)	487, 829, 911, 946, 1489, 1948
BstX2I	RGATCY	3 cut(s)	346, 1021, 1348
BstYI	RGATCY	3 cut(s)	346, 1021, 1348
Bsu15I	ATCGAT	2 cut(s)	425, 1427
Bsu36I	CCTNAGG	1 cut(s)	98
BsuRI	GGCC	7 cut(s)	103, 483, 668, 805, 1485, 1670, 1807
BsuTUI	ATCGAT	2 cut(s)	425, 1427
BtgZI	GCGATG	2 cut(s)	612, 1614
BtrI	CACGTC	1 cut(s)	1993
BtsCI	GGATG	4 cut(s)	328, 640, 1330, 1642
BtsI	GCAGTG	2 cut(s)	861, 1863
BtsIMutI	CAGTG	6 cut(s)	525, 708, 861, 1527, 1710, 1863
Cac8I	GCNNGC	5 cut(s)	80, 161, 355, 1163, 1357
CaiI	CAGNNNCTG	2 cut(s)	551, 1553
CfoI	GCGC	2 cut(s)	359, 1361
Cfr10I	RCCGGY	1 cut(s)	412
Cfr13I	GGNCC	4 cut(s)	6, 482, 1484, 2024
ClaI	ATCGAT	2 cut(s)	425, 1427
Csp6I	GTAC	2 cut(s)	1034, 2036
CspAI	ACCGGT	1 cut(s)	412
CviAII	CATG	4 cut(s)	263, 471, 1013, 1473
CviQI	GTAC	2 cut(s)	1034, 2036
DdeI	CTNAG	6 cut(s)	98, 201, 552, 1137, 1554, 2139
EaeI	YGGCCR	2 cut(s)	803, 1805
Eam1104I	CTCTTC	2 cut(s)	861, 1863
EarI	CTCTTC	2 cut(s)	861, 1863
Eco130I	CCWWGG	3 cut(s)	104, 740, 1742
Eco47I	GGWCC	2 cut(s)	6, 2024
Eco57I	CTGAAG	3 cut(s)	542, 555, 1544
Eco81I	CCTNAGG	1 cut(s)	98
EcoO109I	RGGNCCY	2 cut(s)	6, 2024
EcoRII	CCWGG	6 cut(s)	478, 503, 1036, 1480, 1505, 2038
EcoT14I	CCWWGG	3 cut(s)	104, 740, 1742
ErhI	CCWWGG	3 cut(s)	104, 740, 1742
FaeI	CATG	4 cut(s)	266, 474, 1016, 1476
FatI	CATG	4 cut(s)	262, 470, 1012, 1472
FauI	CCCGC	1 cut(s)	83
FauNDI	CATATG	1 cut(s)	1816
FbaI	TGATCA	2 cut(s)	168, 1170
Fnu4HI	GCNGC	6 cut(s)	476, 843, 900, 960, 1478, 1962
FokI	GGATG	4 cut(s)	335, 647, 1337, 1649
Fsp4HI	GCNGC	6 cut(s)	476, 843, 900, 960, 1478, 1962
FspBI	CTAG	3 cut(s)	513, 1004, 2006
GlaI	GCGC	2 cut(s)	358, 1360
GluI	GCNGC	6 cut(s)	476, 843, 900, 960, 1478, 1962
HaeIII	GGCC	7 cut(s)	103, 483, 668, 805, 1485, 1670, 1807
HapII	CCGG	1 cut(s)	413
HhaI	GCGC	2 cut(s)	359, 1361
Hin1II	CATG	4 cut(s)	266, 474, 1016, 1476
Hin6I	GCGC	2 cut(s)	357, 1359
HinP1I	GCGC	2 cut(s)	357, 1359
HincII	GTYRAC	4 cut(s)	613, 1009, 1615, 2011
HindII	GTYRAC	4 cut(s)	613, 1009, 1615, 2011
HpaI	GTTAAC	4 cut(s)	613, 1009, 1615, 2011
HpaII	CCGG	1 cut(s)	413
HphI	GGTGA	6 cut(s)	671, 688, 1044, 1673, 1690, 2046
Hpy188III	TCNNGA	7 cut(s)	130, 236, 499, 1093, 1238, 1501, 2095
Hpy99I	CGWCG	1 cut(s)	1997
HpyCH4III	ACNGT	6 cut(s)	397, 529, 967, 1399, 1531, 1969
HpyCH4IV	ACGT	2 cut(s)	816, 1992
HpyF10VI	GCNNNNNNNGC	3 cut(s)	851, 1133, 2135
HpyF3I	CTNAG	6 cut(s)	98, 201, 552, 1137, 1554, 2139
HpySE526I	ACGT	2 cut(s)	816, 1992
Hsp92II	CATG	4 cut(s)	266, 474, 1016, 1476
HspAI	GCGC	2 cut(s)	357, 1359
KpnI	GGTACC	2 cut(s)	1037, 2039
Ksp22I	TGATCA	2 cut(s)	168, 1170
KspAI	GTTAAC	4 cut(s)	613, 1009, 1615, 2011
LmnI	GCTCC	2 cut(s)	1124, 2126
Lsp1109I	GCAGC	6 cut(s)	487, 829, 911, 946, 1489, 1948
LweI	GCATC	2 cut(s)	538, 1540
MaeI	CTAG	3 cut(s)	513, 1004, 2006
MaeII	ACGT	2 cut(s)	816, 1992
MaeIII	GTNAC	3 cut(s)	704, 1706, 2161
MflI	RGATCY	3 cut(s)	346, 1021, 1348
MhlI	GDGCHC	3 cut(s)	293, 986, 1295
MlsI	TGGCCA	2 cut(s)	805, 1807
MluCI	AATT	8 cut(s)	133, 209, 231, 269, 753, 1211, 1233, 1755
MluNI	TGGCCA	2 cut(s)	805, 1807
MlyI	GAGTC	3 cut(s)	25, 1039, 2041
MmeI	TCCRAC	2 cut(s)	1102, 2104
Mox20I	TGGCCA	2 cut(s)	805, 1807
MscI	TGGCCA	2 cut(s)	805, 1807
MseI	TTAA	6 cut(s)	612, 861, 1008, 1614, 1863, 2010
Msp20I	TGGCCA	2 cut(s)	805, 1807
MspA1I	CMGCKG	3 cut(s)	652, 1058, 2060
MspI	CCGG	1 cut(s)	413
MspR9I	CCNGG	6 cut(s)	480, 505, 1038, 1482, 1507, 2040
MvaI	CCWGG	6 cut(s)	480, 505, 1038, 1482, 1507, 2040
MvnI	CGCG	1 cut(s)	78
MwoI	GCNNNNNNNGC	3 cut(s)	851, 1133, 2135
NdeI	CATATG	1 cut(s)	1816
NlaIII	CATG	4 cut(s)	266, 474, 1016, 1476
NlaIV	GGNNCC	9 cut(s)	8, 87, 119, 348, 822, 1035, 1126, 1350, 2037
PflFI	GACNNNGTC	1 cut(s)	10
PinAI	ACCGGT	1 cut(s)	412
PkrI	GCNGC	6 cut(s)	477, 844, 901, 961, 1479, 1963
PleI	GAGTC	3 cut(s)	24, 1039, 2041
PpsI	GAGTC	3 cut(s)	24, 1039, 2041
Ppu21I	YACGTR	1 cut(s)	817
PpuMI	RGGWCCY	2 cut(s)	6, 2024
Psp5II	RGGWCCY	2 cut(s)	6, 2024
Psp6I	CCWGG	6 cut(s)	478, 503, 1036, 1480, 1505, 2038
PspGI	CCWGG	6 cut(s)	478, 503, 1036, 1480, 1505, 2038
PspN4I	GGNNCC	9 cut(s)	8, 87, 119, 348, 822, 1035, 1126, 1350, 2037
PspPI	GGNCC	4 cut(s)	6, 482, 1484, 2024
PspPPI	RGGWCCY	2 cut(s)	6, 2024
PstI	CTGCAG	3 cut(s)	856, 901, 1858
PstNI	CAGNNNCTG	2 cut(s)	551, 1553
PsuI	RGATCY	3 cut(s)	346, 1021, 1348
PsyI	GACNNNGTC	1 cut(s)	10
PvuII	CAGCTG	3 cut(s)	652, 1058, 2060
RsaI	GTAC	2 cut(s)	1035, 2037
RsaNI	GTAC	2 cut(s)	1034, 2036
SaqAI	TTAA	6 cut(s)	612, 861, 1008, 1614, 1863, 2010
SatI	GCNGC	6 cut(s)	476, 843, 900, 960, 1478, 1962
Sau96I	GGNCC	4 cut(s)	6, 482, 1484, 2024
SchI	GAGTC	3 cut(s)	25, 1039, 2041
ScrFI	CCNGG	6 cut(s)	480, 505, 1038, 1482, 1507, 2040
SduI	GDGCHC	3 cut(s)	293, 986, 1295
SfaNI	GCATC	2 cut(s)	538, 1540
SfcI	CTRYAG	4 cut(s)	852, 897, 1854, 1899
SinI	GGWCC	2 cut(s)	6, 2024
SmlI	CTYRAG	2 cut(s)	907, 1909
SmoI	CTYRAG	2 cut(s)	907, 1909
Sse9I	AATT	8 cut(s)	133, 209, 231, 269, 753, 1211, 1233, 1755
SsiI	CCGC	3 cut(s)	35, 76, 1944
SspMI	CTAG	3 cut(s)	513, 1004, 2006
StyD4I	CCNGG	6 cut(s)	478, 503, 1036, 1480, 1505, 2038
StyI	CCWWGG	3 cut(s)	104, 740, 1742
TaaI	ACNGT	6 cut(s)	397, 529, 967, 1399, 1531, 1969
TaiI	ACGT	2 cut(s)	819, 1995
TaqI	TCGA	8 cut(s)	14, 26, 425, 993, 1092, 1427, 1995, 2094
TaqII	GACCGA	2 cut(s)	190, 1192
TasI	AATT	8 cut(s)	133, 209, 231, 269, 753, 1211, 1233, 1755
Tru1I	TTAA	6 cut(s)	612, 861, 1008, 1614, 1863, 2010
Tru9I	TTAA	6 cut(s)	612, 861, 1008, 1614, 1863, 2010
TscAI	CASTG	6 cut(s)	532, 715, 861, 1534, 1717, 1863
TseI	GCWGC	6 cut(s)	475, 842, 899, 959, 1477, 1961
TspRI	CASTG	6 cut(s)	532, 715, 861, 1534, 1717, 1863
Tth111I	GACNNNGTC	1 cut(s)	10
VpaK11BI	GGWCC	2 cut(s)	6, 2024
XapI	RAATTY	4 cut(s)	133, 231, 269, 1233
XspI	CTAG	3 cut(s)	513, 1004, 2006

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.