Rh5BG471900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002509
Physical Location & Seq
Forward (+)
75106090 .. 75107762
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG471900.1

Sequence Viewer

Length: 1107 bp
ATGTCAGATCCGTACCCTTCTCATTTCTCATGCCTATATGCCTGTTATACATATAGCTTTGTTCATTTTATATCATTAGGACTCTCTCCCTTTCTGATACAACGGAGCCAAATGGAAGCCTTTGCAAGATCTTCCCCAACGTTGTTTGCAAAGGCTAATACTAACAAGCGTCCATCGCCTGTAAAAGCTCTTCTGAGTCAATCTAGCATTTGCTTTGTAAGGGTTTGGAATTCAAATGCAAATGTTCATCATCATACTTCTTTGCTTAGGCACAAAGCTATTCGATCGGTTTCCTCATCTGAAACAAGGGTGTTAAGGAACCCAGACTCAGCAGGAATTGGGATCGAACCAGCAGCGCCATCAAAAACCGTTCATGTCCAATTCCAATTACACAAGAAGTGCCGCTTCGGTGATAATTTTCTCTTAGTAGGGAATGAACCTATCATTGGACAGTGGAACCCTTCAAGTGCTATACCCCTTAACTGGTCAGATGGAAACATTTGGACAGTTGACCTGGACATACCAATTGACAGAGCAATCCAGTTCAAGTTTATACTAAAGAAAAGAACAGGAGATCTGTTGTGGCAACCTGGTCCAGACCGAATTCTTCACACCTGGGATACCAACAGCACTATAACCATAGCCGAAGACTGGAAAAATGCCAAACTTCAGAAATTAACTGAAAAGAAAATTGTCAGTCAAAACGAGACGCTACTTACAAACCAGGCTTATAGCATACCAACTTATTATAGCAACAAGAATGAGTTCGCTTTCAAAGAGAGGGCGTTTGGTTCTGAAAAGGGAACTCTGCTTCAGGAAAGCCAAGCAGTTTGCATTGCAGATGACGGTGTTTGCATCATGACAAGACCAGCATCCTTAGGAAGCATGGATGAAGATGAATACTGTGAAGTTGAATATTATGACGATGAATATTACGATGATGACTGTGAATATGAAGATAGATATGAAGACGAAGACATCATGTTTACTTATGAAGGAGGCCGTGTTCTAGTGCCTGGTTTAGTACAAGGTGTAACTGGAAAAACCATTGATATCAGTGCTGATGGCTCGATTGTGATTGATGCGAGAGGGGAACACAATTCTTAA

Protein Analysis

368

Amino Acids

41.54

Weight (kDa)

5.33

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_20 PF00686 123 - 211 3.1e-19 Starch binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 403
AclI AACGTT 1 cut(s) 140
AclWI GGATC 2 cut(s) 2, 350
AcsI RAATTY 2 cut(s) 229, 603
AcuI CTGAAG 2 cut(s) 653, 797
AfaI GTAC 2 cut(s) 14, 1026
AfiI CCNNNNNNNGG 3 cut(s) 446, 483, 651
AgsI TTSAA 5 cut(s) 234, 465, 547, 775, 914
AjnI CCWGG 5 cut(s) 513, 589, 614, 723, 1015
AjuI GAANNNNNNNTTGG 2 cut(s) 429, 461
AloI GAACNNNNNNTCC 2 cut(s) 990, 1022
AluBI AGCT 3 cut(s) 57, 188, 278
AluI AGCT 3 cut(s) 57, 188, 278
Alw26I GTCTC 1 cut(s) 701
AlwI GGATC 2 cut(s) 2, 350
AoxI GGCC 1 cut(s) 1000
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 2 cut(s) 229, 603
Asp700I GAANNNNTTC 1 cut(s) 764
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 593
AsuHPI GGTGA 1 cut(s) 422
AvaII GGWCC 1 cut(s) 593
AxyI CCTNAGG 1 cut(s) 877
BarI GAAGNNNNNNTAC 2 cut(s) 174, 206
BbsI GAAGAC 3 cut(s) 654, 975, 981
BbvI GCAGC 1 cut(s) 365
BccI CCATC 4 cut(s) 181, 367, 485, 1058
BceAI ACGGC 1 cut(s) 987
BciT130I CCWGG 5 cut(s) 515, 591, 616, 725, 1017
BciVI GTATCC 1 cut(s) 613
BcoDI GTCTC 1 cut(s) 701
BfaI CTAG 2 cut(s) 204, 1010
BfoI RGCGCY 1 cut(s) 359
BfuI GTATCC 1 cut(s) 613
BglII AGATCT 2 cut(s) 128, 574
BisI GCNGC 2 cut(s) 354, 403
BlsI GCNGC 2 cut(s) 355, 404
Bme1390I CCNGG 5 cut(s) 515, 591, 616, 725, 1017
Bme18I GGWCC 1 cut(s) 593
BmgT120I GGNCC 1 cut(s) 593
BmiI GGNNCC 3 cut(s) 107, 320, 458
BmrFI CCNGG 5 cut(s) 515, 591, 616, 725, 1017
BmsI GCATC 3 cut(s) 864, 881, 1072
BpiI GAAGAC 3 cut(s) 654, 975, 981
Bpu10I CCTNAGC 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 615
BsaXI ACNNNNNCTCC 2 cut(s) 990, 1020
Bsc4I CCNNNNNNNGG 3 cut(s) 446, 483, 651
Bse1I ACTGG 4 cut(s) 488, 541, 656, 1042
Bse21I CCTNAGG 1 cut(s) 877
Bse3DI GCAATG 1 cut(s) 834
BseBI CCWGG 5 cut(s) 515, 591, 616, 725, 1017
BseDI CCNNGG 1 cut(s) 615
BseGI GGATG 2 cut(s) 872, 895
BseLI CCNNNNNNNGG 3 cut(s) 446, 483, 651
BseMI GCAATG 1 cut(s) 834
BseMII CTCAG 2 cut(s) 185, 342
BseNI ACTGG 4 cut(s) 488, 541, 656, 1042
BseXI GCAGC 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 287
BshFI GGCC 1 cut(s) 1002
BsiEI CGRYCG 1 cut(s) 287
BslI CCNNNNNNNGG 3 cut(s) 446, 483, 651
BsmAI GTCTC 1 cut(s) 701
BsmBI CGTCTC 1 cut(s) 701
BsnI GGCC 1 cut(s) 1002
Bsp143I GATC 5 cut(s) 7, 128, 284, 342, 574
BspACI CCGC 1 cut(s) 403
BspANI GGCC 1 cut(s) 1002
BspCNI CTCAG 2 cut(s) 186, 341
BspHI TCATGA 1 cut(s) 858
BspLI GGNNCC 3 cut(s) 107, 320, 458
BspPI GGATC 2 cut(s) 2, 350
BspQI GCTCTTC 1 cut(s) 195
BsrDI GCAATG 1 cut(s) 834
BsrI ACTGG 4 cut(s) 488, 541, 656, 1042
BssECI CCNNGG 1 cut(s) 615
BssMI GATC 5 cut(s) 7, 128, 284, 342, 574
Bst2UI CCWGG 5 cut(s) 515, 591, 616, 725, 1017
Bst4CI ACNGT 6 cut(s) 370, 453, 508, 848, 905, 947
Bst6I CTCTTC 1 cut(s) 195
BstDEI CTNAG 5 cut(s) 194, 266, 328, 424, 877
BstF5I GGATG 2 cut(s) 872, 895
BstH2I RGCGCY 1 cut(s) 359
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 5 cut(s) 10, 131, 287, 345, 577
BstMAI GTCTC 1 cut(s) 701
BstMBI GATC 5 cut(s) 7, 128, 284, 342, 574
BstMCI CGRYCG 1 cut(s) 287
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 5 cut(s) 515, 591, 616, 725, 1017
BstSCI CCNGG 5 cut(s) 513, 589, 614, 723, 1015
BstV1I GCAGC 1 cut(s) 365
BstV2I GAAGAC 3 cut(s) 654, 975, 981
BstX2I RGATCY 3 cut(s) 7, 128, 574
BstYI RGATCY 3 cut(s) 7, 128, 574
Bsu36I CCTNAGG 1 cut(s) 877
BsuI GTATCC 1 cut(s) 613
BsuRI GGCC 1 cut(s) 1002
BtgZI GCGATG 1 cut(s) 159
BtsCI GGATG 2 cut(s) 872, 895
BtsIMutI CAGTG 2 cut(s) 458, 1063
CciI TCATGA 1 cut(s) 858
CfoI GCGC 1 cut(s) 358
Cfr13I GGNCC 1 cut(s) 593
CseI GACGC 2 cut(s) 158, 718
CsiI ACCWGGT 1 cut(s) 589
Csp6I GTAC 2 cut(s) 13, 1025
CviAII CATG 5 cut(s) 30, 374, 859, 886, 982
CviQI GTAC 2 cut(s) 13, 1025
DdeI CTNAG 5 cut(s) 194, 266, 328, 424, 877
DpnI GATC 5 cut(s) 9, 130, 286, 344, 576
DpnII GATC 5 cut(s) 7, 128, 284, 342, 574
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco32I GATATC 1 cut(s) 1054
Eco47I GGWCC 1 cut(s) 593
Eco57I CTGAAG 2 cut(s) 653, 797
Eco81I CCTNAGG 1 cut(s) 877
EcoRI GAATTC 2 cut(s) 229, 603
EcoRII CCWGG 5 cut(s) 513, 589, 614, 723, 1015
EcoRV GATATC 1 cut(s) 1054
Esp3I CGTCTC 1 cut(s) 701
FaeI CATG 5 cut(s) 33, 377, 862, 889, 985
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FatI CATG 5 cut(s) 29, 373, 858, 885, 981
Fnu4HI GCNGC 2 cut(s) 354, 403
FokI GGATG 2 cut(s) 859, 902
Fsp4HI GCNGC 2 cut(s) 354, 403
FspBI CTAG 2 cut(s) 204, 1010
GlaI GCGC 1 cut(s) 357
GluI GCNGC 2 cut(s) 354, 403
HaeII RGCGCY 1 cut(s) 359
HaeIII GGCC 1 cut(s) 1002
HgaI GACGC 2 cut(s) 158, 718
HhaI GCGC 1 cut(s) 358
Hin1II CATG 5 cut(s) 33, 377, 862, 889, 985
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HinfI GANTC 3 cut(s) 81, 196, 326
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 2 cut(s) 511, 987
Hpy188I TCNGA 7 cut(s) 7, 96, 195, 301, 490, 672, 796
Hpy188III TCNNGA 3 cut(s) 596, 815, 859
Hpy8I GTNNAC 2 cut(s) 511, 987
HpyAV CCTTC 3 cut(s) 27, 471, 989
HpyCH4III ACNGT 6 cut(s) 370, 453, 508, 848, 905, 947
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 6 cut(s) 125, 149, 239, 834, 839, 855
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 5 cut(s) 194, 266, 328, 424, 877
HpySE526I ACGT 1 cut(s) 140
Hsp92II CATG 5 cut(s) 33, 377, 862, 889, 985
HspAI GCGC 1 cut(s) 356
Kzo9I GATC 5 cut(s) 7, 128, 284, 342, 574
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 1 cut(s) 105
Lsp1109I GCAGC 1 cut(s) 365
LweI GCATC 3 cut(s) 864, 881, 1072
MabI ACCWGGT 1 cut(s) 589
MaeI CTAG 2 cut(s) 204, 1010
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 1 cut(s) 1033
MalI GATC 5 cut(s) 9, 130, 286, 344, 576
MboI GATC 5 cut(s) 7, 128, 284, 342, 574
MboII GAAGA 8 cut(s) 123, 182, 599, 659, 905, 968, 980, 986
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 3 cut(s) 7, 128, 574
MlyI GAGTC 3 cut(s) 75, 205, 320
MnlI CCTC 4 cut(s) 304, 774, 992, 1082
MroXI GAANNNNTTC 1 cut(s) 764
MseI TTAA 4 cut(s) 314, 480, 677, 1105
MspR9I CCNGG 5 cut(s) 515, 591, 616, 725, 1017
MunI CAATTG 1 cut(s) 525
MvaI CCWGG 5 cut(s) 515, 591, 616, 725, 1017
MwoI GCNNNNNNNGC 1 cut(s) 175
NdeII GATC 5 cut(s) 7, 128, 284, 342, 574
NlaIII CATG 5 cut(s) 33, 377, 862, 889, 985
NlaIV GGNNCC 3 cut(s) 107, 320, 458
PagI TCATGA 1 cut(s) 858
PciSI GCTCTTC 1 cut(s) 195
PdmI GAANNNNTTC 1 cut(s) 764
PkrI GCNGC 2 cut(s) 355, 404
Ple19I CGATCG 1 cut(s) 287
PleI GAGTC 3 cut(s) 75, 204, 320
PpsI GAGTC 3 cut(s) 75, 204, 320
Psp1406I AACGTT 1 cut(s) 140
Psp6I CCWGG 5 cut(s) 513, 589, 614, 723, 1015
PspGI CCWGG 5 cut(s) 513, 589, 614, 723, 1015
PspN4I GGNNCC 3 cut(s) 107, 320, 458
PspPI GGNCC 1 cut(s) 593
PsuI RGATCY 3 cut(s) 7, 128, 574
PvuI CGATCG 1 cut(s) 287
RsaI GTAC 2 cut(s) 14, 1026
RsaNI GTAC 2 cut(s) 13, 1025
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 4 cut(s) 314, 480, 677, 1105
SatI GCNGC 2 cut(s) 354, 403
Sau3AI GATC 5 cut(s) 7, 128, 284, 342, 574
Sau96I GGNCC 1 cut(s) 593
SchI GAGTC 3 cut(s) 75, 205, 320
ScrFI CCNGG 5 cut(s) 515, 591, 616, 725, 1017
SetI ASST 9 cut(s) 59, 143, 190, 280, 442, 516, 592, 617, 1033
SexAI ACCWGGT 1 cut(s) 589
SfaNI GCATC 3 cut(s) 864, 881, 1072
SinI GGWCC 1 cut(s) 593
SsiI CCGC 1 cut(s) 403
SspI AATATT 2 cut(s) 917, 932
SspMI CTAG 2 cut(s) 204, 1010
StyD4I CCNGG 5 cut(s) 513, 589, 614, 723, 1015
TaaI ACNGT 6 cut(s) 370, 453, 508, 848, 905, 947
TaiI ACGT 1 cut(s) 143
TaqI TCGA 3 cut(s) 283, 345, 1070
TaqII GACCGA 1 cut(s) 615
TatI WGTACW 1 cut(s) 1024
TauI GCSGC 1 cut(s) 405
Tru1I TTAA 4 cut(s) 314, 480, 677, 1105
Tru9I TTAA 4 cut(s) 314, 480, 677, 1105
TscAI CASTG 2 cut(s) 458, 1063
TseI GCWGC 1 cut(s) 353
TspGWI ACGGA 1 cut(s) 118
TspRI CASTG 2 cut(s) 458, 1063
VpaK11BI GGWCC 1 cut(s) 593
XapI RAATTY 2 cut(s) 229, 603
XmnI GAANNNNTTC 1 cut(s) 764
XspI CTAG 2 cut(s) 204, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.