AT5G01960

RING finger and transmembrane domain-containing protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003923
Physical Location & Seq
Forward (+)
370239 .. 372969
2731 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01960.1

Sequence Viewer

Length: 1281 bp
ATGGAAACGCCCGGCGCCAGTCATGATTCATTTAGGGGTTCTCCTCGTAGAAACTCCATCCTCTCTGCTTCTAACATCATCCAGGCCCCTATTTCTACCTTGTTAGAGTATTCGGGTCTCTTTCGAGCTAGGCCAAGTCCTAGTCATGAGGCTGAGACGCTTGTTAGTGATGATTCTTCAGGACTCTCCAATGGGGAGGTTGCCATTAGGATCATTGGCAACACCGAGCAGGATGCTGAAACTGATACCAATGCTCTGAGGGAACCCGGACACTCTGAACTCTTGGGTTCTTCTGCTACTCAGGTTGATCCCATGGGAGGTGCTTCAGAAGGTGCCTCTCAGGCTGCTCCAGGTGATCCTGCTGCTGGAGACGCCGCTTCCCGGGATTCGCCTTACCAAAGATATGATATTCAGCAGGCTGCACGCTGGATTGAGCAAATTCTTCCCTTTTCTTTGCTTCTGTTGGTTGTTTTTATCCGCCAGCATTTGCAGGGGTTCTTTGTCGCCATTTGGATTGCTGCTGTCATGTTCAAGTCAAATGATATTCTCAAGAAGCAAACAGCTTTGAAGGGTGAGAGGCATATCTCGGCATTAATTGGAATCTCTGTAGCATTTACGGCCCATGTTGTTGGTGTTTATTGGTGGTTTCGGAAGGATGACCTCTTGTATCCCTTAATCATGCTGCCTCCAAAGTCCATACCACCTTTCTGGCATGCCATTTTTATCATAGTGGTGAATGATACACTTGTCCGGCAGGCCTCAATGATTTTCAAGTGTTTTTTGTTGATGTACTACAAAAACAGTAGGGGTCGGAATTATCGTAAACAGGGTCAATTGCTAACTTTGGTTGAATATTTCATGCTTCTGTATCGCTCTTTACTGCCTACCCCTGTATGGTACCGTTTCTTTTTGAACAAGGATTATGGAAGCCTCTTCTCCTCTCTTATGACGGGATTGTATCTTACATTCAAGCTCACTTCAGTAGTTGAGAAGGTTCAATCCTTCTTTACGGCATTGAAGGCTTTGTCACGTAAAGAGGTGCACTACGGATCATATGCAACTACAGAGCAGGTAAATGCAGCAGGCGACCTGTGTGCCATCTGCCAGGAGAAGATGCACACCCCAATTCTTCTTCGCTGCAAACACATGTTTTGCGAAGATTGCGTTTCAGAGTGGTTTGAGAGAGAGAGGACGTGTCCTTTGTGCAGGGCCTTGGTCAAACCAGCAGATCTGAAATCATTCGGTGATGGATCCACCAGCCTCTTCTTCCAGATTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3 zf-RING_2

Protein Analysis

426

Amino Acids

47.79

Weight (kDa)

8.05

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 362 - 403 5.8e-06 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 363 - 406 1.2e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 364 - 403 2.9e-10 Ring finger domain
zf-C3HC4 PF00097 365 - 402 1.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 365 - 402 3.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 365 - 400 7.2e-08 RING-type zinc-finger
zf-RING_5 PF14634 365 - 403 1.1e-06 zinc-RING finger domain
Prok-RING_4 PF14447 365 - 408 9.4e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1060
Acc65I GGTACC 1 cut(s) 897
AccB1I GGYRCC 3 cut(s) 14, 332, 897
AciI CCGC 2 cut(s) 375, 478
AclWI GGATC 6 cut(s) 218, 302, 350, 1057, 1245, 1258
AcsI RAATTY 1 cut(s) 438
AcuI CTGAAG 3 cut(s) 162, 309, 963
AcyI GRCGYC 2 cut(s) 15, 372
AfaI GTAC 2 cut(s) 791, 899
AfiI CCNNNNNNNGG 4 cut(s) 317, 365, 381, 894
AflIII ACRYGT 2 cut(s) 1146, 1193
AgsI TTSAA 8 cut(s) 532, 568, 772, 851, 913, 970, 998, 1018
AjiI CACGTC 1 cut(s) 1194
AjnI CCWGG 3 cut(s) 81, 349, 1104
AluBI AGCT 3 cut(s) 128, 563, 973
AluI AGCT 3 cut(s) 128, 563, 973
Alw21I GWGCWC 1 cut(s) 1044
Alw26I GTCTC 3 cut(s) 122, 149, 363
Alw44I GTGCAC 1 cut(s) 1040
AlwI GGATC 6 cut(s) 218, 302, 350, 1057, 1245, 1258
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 5 cut(s) 84, 131, 618, 756, 1209
ApaLI GTGCAC 1 cut(s) 1040
ApeKI GCWGC 7 cut(s) 344, 362, 419, 518, 682, 1079, 1137
ApoI RAATTY 1 cut(s) 438
ArsI GACNNNNNNTTYG 2 cut(s) 1183, 1215
AseI ATTAAT 1 cut(s) 593
Asp700I GAANNNNTTC 1 cut(s) 1238
Asp718I GGTACC 1 cut(s) 897
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 3 cut(s) 85, 619, 1209
AsuC2I CCSGG 4 cut(s) 12, 267, 382, 383
AsuHPI GGTGA 4 cut(s) 365, 584, 745, 1256
AvaI CYCGRG 1 cut(s) 381
BaeGI GKGCMC 1 cut(s) 1044
BaeI ACNNNNGTAYC 2 cut(s) 732, 765
BamHI GGATCC 1 cut(s) 1250
BanI GGYRCC 3 cut(s) 14, 332, 897
Bbv12I GWGCWC 1 cut(s) 1044
BbvI GCAGC 7 cut(s) 331, 349, 406, 505, 669, 1091, 1124
BccI CCATC 3 cut(s) 65, 1106, 1241
BceAI ACGGC 2 cut(s) 633, 1026
BcgI CGANNNNNNTGC 4 cut(s) 215, 249, 1076, 1110
BciT130I CCWGG 3 cut(s) 83, 351, 1106
BciVI GTATCC 1 cut(s) 678
BcnI CCSGG 4 cut(s) 12, 267, 382, 383
BcoDI GTCTC 3 cut(s) 122, 149, 363
BfaI CTAG 2 cut(s) 129, 141
BfmI CTRYAG 2 cut(s) 606, 1062
BfoI RGCGCY 1 cut(s) 18
BfuAI ACCTGC 1 cut(s) 1060
BfuI GTATCC 1 cut(s) 678
BglI GCCNNNNNGGC 1 cut(s) 341
BglII AGATCT 1 cut(s) 1228
BisI GCNGC 8 cut(s) 345, 363, 375, 420, 519, 683, 1080, 1138
BlsI GCNGC 8 cut(s) 346, 364, 376, 421, 520, 684, 1081, 1139
Bme1390I CCNGG 7 cut(s) 12, 83, 267, 351, 382, 383, 1106
BmeT110I CYCGRG 1 cut(s) 381
BmgBI CACGTC 1 cut(s) 1194
BmgT120I GGNCC 3 cut(s) 85, 619, 1209
BmiI GGNNCC 6 cut(s) 16, 87, 264, 334, 899, 1252
BmrFI CCNGG 7 cut(s) 12, 83, 267, 351, 382, 383, 1106
BmsI GCATC 2 cut(s) 223, 1104
BpmI CTGGAG 2 cut(s) 333, 387
BpuEI CTTGAG 1 cut(s) 533
BpuMI CCSGG 4 cut(s) 12, 267, 382, 383
BsaAI YACGTR 1 cut(s) 1031
BsaHI GRCGYC 2 cut(s) 15, 372
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 3 cut(s) 312, 381, 1212
Bsc4I CCNNNNNNNGG 4 cut(s) 317, 365, 381, 894
Bse1I ACTGG 1 cut(s) 18
BseBI CCWGG 3 cut(s) 83, 351, 1106
BseDI CCNNGG 3 cut(s) 312, 381, 1212
BseGI GGATG 4 cut(s) 57, 78, 238, 661
BseLI CCNNNNNNNGG 4 cut(s) 317, 365, 381, 894
BseMII CTCAG 4 cut(s) 144, 248, 314, 353
BseNI ACTGG 1 cut(s) 18
BseRI GAGGAG 2 cut(s) 33, 928
BseSI GKGCMC 1 cut(s) 1044
BseXI GCAGC 7 cut(s) 331, 349, 406, 505, 669, 1091, 1124
BsgI GTGCAG 2 cut(s) 405, 1225
BshFI GGCC 5 cut(s) 86, 133, 620, 758, 1211
BshNI GGYRCC 3 cut(s) 14, 332, 897
BsiHKAI GWGCWC 1 cut(s) 1044
BsiHKCI CYCGRG 1 cut(s) 381
BsiSI CCGG 4 cut(s) 12, 267, 382, 751
BslI CCNNNNNNNGG 4 cut(s) 317, 365, 381, 894
BsmAI GTCTC 3 cut(s) 122, 149, 363
BsmBI CGTCTC 2 cut(s) 149, 363
BsnI GGCC 5 cut(s) 86, 133, 620, 758, 1211
Bso31I GGTCTC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 381
Bsp1286I GDGCHC 1 cut(s) 1044
Bsp143I GATC 6 cut(s) 210, 307, 355, 1049, 1228, 1250
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 2 cut(s) 375, 478
BspANI GGCC 5 cut(s) 86, 133, 620, 758, 1211
BspCNI CTCAG 4 cut(s) 145, 249, 313, 352
BspHI TCATGA 2 cut(s) 22, 145
BspLI GGNNCC 6 cut(s) 16, 87, 264, 334, 899, 1252
BspMI ACCTGC 1 cut(s) 1060
BspPI GGATC 6 cut(s) 218, 302, 350, 1057, 1245, 1258
BspT107I GGYRCC 3 cut(s) 14, 332, 897
BspTNI GGTCTC 1 cut(s) 122
BsrI ACTGG 1 cut(s) 18
BssECI CCNNGG 3 cut(s) 312, 381, 1212
BssMI GATC 6 cut(s) 210, 307, 355, 1049, 1228, 1250
BssNI GRCGYC 2 cut(s) 15, 372
BssT1I CCWWGG 2 cut(s) 312, 1212
Bst2UI CCWGG 3 cut(s) 83, 351, 1106
Bst4CI ACNGT 2 cut(s) 803, 902
Bst6I CTCTTC 2 cut(s) 938, 1268
BstACI GRCGYC 2 cut(s) 15, 372
BstBAI YACGTR 1 cut(s) 1031
BstC8I GCNNGC 6 cut(s) 417, 424, 482, 714, 756, 1084
BstDEI CTNAG 4 cut(s) 153, 257, 300, 339
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 4 cut(s) 57, 78, 238, 661
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 6 cut(s) 213, 310, 358, 1052, 1231, 1253
BstMAI GTCTC 3 cut(s) 122, 149, 363
BstMBI GATC 6 cut(s) 210, 307, 355, 1049, 1228, 1250
BstMWI GCNNNNNNNGC 5 cut(s) 341, 371, 617, 1019, 1161
BstNI CCWGG 3 cut(s) 83, 351, 1106
BstNSI RCATGY 2 cut(s) 716, 1150
BstSCI CCNGG 7 cut(s) 10, 81, 265, 349, 380, 381, 1104
BstSFI CTRYAG 2 cut(s) 606, 1062
BstSLI GKGCMC 1 cut(s) 1044
BstV1I GCAGC 7 cut(s) 331, 349, 406, 505, 669, 1091, 1124
BstX2I RGATCY 2 cut(s) 1228, 1250
BstXI CCANNNNNNTGG 2 cut(s) 629, 708
BstYI RGATCY 2 cut(s) 1228, 1250
BsuI GTATCC 1 cut(s) 678
BsuRI GGCC 5 cut(s) 86, 133, 620, 758, 1211
BtgI CCRYGG 1 cut(s) 312
BtrI CACGTC 1 cut(s) 1194
BtsCI GGATG 4 cut(s) 57, 78, 238, 661
BveI ACCTGC 1 cut(s) 1060
Cac8I GCNNGC 6 cut(s) 417, 424, 482, 714, 756, 1084
CciI TCATGA 2 cut(s) 22, 145
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 3 cut(s) 85, 619, 1209
Cfr9I CCCGGG 1 cut(s) 381
CseI GACGC 2 cut(s) 166, 380
Csp6I GTAC 2 cut(s) 790, 898
CviAII CATG 9 cut(s) 23, 146, 313, 526, 623, 679, 713, 859, 1147
CviQI GTAC 2 cut(s) 790, 898
DdeI CTNAG 4 cut(s) 153, 257, 300, 339
DinI GGCGCC 1 cut(s) 16
DpnI GATC 6 cut(s) 212, 309, 357, 1051, 1230, 1252
DpnII GATC 6 cut(s) 210, 307, 355, 1049, 1228, 1250
Eam1104I CTCTTC 2 cut(s) 938, 1268
EarI CTCTTC 2 cut(s) 938, 1268
EciI GGCGGA 1 cut(s) 467
Eco130I CCWWGG 2 cut(s) 312, 1212
Eco147I AGGCCT 1 cut(s) 758
Eco31I GGTCTC 1 cut(s) 122
Eco57I CTGAAG 3 cut(s) 162, 309, 963
Eco88I CYCGRG 1 cut(s) 381
EcoO109I RGGNCCY 2 cut(s) 85, 1209
EcoRII CCWGG 3 cut(s) 81, 349, 1104
EcoT14I CCWWGG 2 cut(s) 312, 1212
EgeI GGCGCC 1 cut(s) 16
EheI GGCGCC 1 cut(s) 16
ErhI CCWWGG 2 cut(s) 312, 1212
Esp3I CGTCTC 2 cut(s) 149, 363
FaeI CATG 9 cut(s) 26, 149, 316, 529, 626, 682, 716, 862, 1150
FatI CATG 9 cut(s) 22, 145, 312, 525, 622, 678, 712, 858, 1146
FauNDI CATATG 1 cut(s) 1054
Fnu4HI GCNGC 8 cut(s) 345, 363, 375, 420, 519, 683, 1080, 1138
FokI GGATG 4 cut(s) 44, 65, 245, 668
Fsp4HI GCNGC 8 cut(s) 345, 363, 375, 420, 519, 683, 1080, 1138
FspBI CTAG 2 cut(s) 129, 141
GlaI GCGC 1 cut(s) 16
GluI GCNGC 8 cut(s) 345, 363, 375, 420, 519, 683, 1080, 1138
GsuI CTGGAG 2 cut(s) 333, 387
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 5 cut(s) 86, 133, 620, 758, 1211
HapII CCGG 4 cut(s) 12, 267, 382, 751
HgaI GACGC 2 cut(s) 166, 380
HhaI GCGC 1 cut(s) 17
Hin1I GRCGYC 2 cut(s) 15, 372
Hin1II CATG 9 cut(s) 26, 149, 316, 529, 626, 682, 716, 862, 1150
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HinfI GANTC 5 cut(s) 26, 173, 183, 386, 600
HpaII CCGG 4 cut(s) 12, 267, 382, 751
HphI GGTGA 4 cut(s) 365, 584, 745, 1256
Hpy166II GTNNAC 2 cut(s) 824, 1042
Hpy188I TCNGA 8 cut(s) 258, 277, 328, 651, 813, 1171, 1233, 1280
Hpy188III TCNNGA 5 cut(s) 23, 146, 180, 550, 1270
Hpy8I GTNNAC 2 cut(s) 824, 1042
HpyAV CCTTC 6 cut(s) 323, 562, 646, 985, 1012, 1012
HpyCH4III ACNGT 2 cut(s) 803, 902
HpyCH4IV ACGT 2 cut(s) 1030, 1193
HpyCH4V TGCA 8 cut(s) 422, 490, 1042, 1058, 1079, 1117, 1140, 1206
HpyF10VI GCNNNNNNNGC 5 cut(s) 341, 371, 617, 1019, 1161
HpyF3I CTNAG 4 cut(s) 153, 257, 300, 339
HpySE526I ACGT 2 cut(s) 1030, 1193
Hsp92I GRCGYC 2 cut(s) 15, 372
Hsp92II CATG 9 cut(s) 26, 149, 316, 529, 626, 682, 716, 862, 1150
HspAI GCGC 1 cut(s) 15
KasI GGCGCC 1 cut(s) 14
KpnI GGTACC 1 cut(s) 901
Kzo9I GATC 6 cut(s) 210, 307, 355, 1049, 1228, 1250
LmnI GCTCC 1 cut(s) 352
Lsp1109I GCAGC 7 cut(s) 331, 349, 406, 505, 669, 1091, 1124
LweI GCATC 2 cut(s) 223, 1104
MaeI CTAG 2 cut(s) 129, 141
MaeII ACGT 2 cut(s) 1030, 1193
MaeIII GTNAC 1 cut(s) 1026
MalI GATC 6 cut(s) 212, 309, 357, 1051, 1230, 1252
MboI GATC 6 cut(s) 210, 307, 355, 1049, 1228, 1250
MfeI CAATTG 1 cut(s) 833
MflI RGATCY 2 cut(s) 1228, 1250
MhlI GDGCHC 1 cut(s) 1044
MluCI AATT 5 cut(s) 438, 594, 814, 833, 1125
Mly113I GGCGCC 1 cut(s) 15
MlyI GAGTC 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 791
MroXI GAANNNNTTC 1 cut(s) 1238
MseI TTAA 2 cut(s) 593, 674
MslI CAYNNNNRTG 1 cut(s) 731
MspI CCGG 4 cut(s) 12, 267, 382, 751
MspR9I CCNGG 7 cut(s) 12, 83, 267, 351, 382, 383, 1106
MunI CAATTG 1 cut(s) 833
MvaI CCWGG 3 cut(s) 83, 351, 1106
MwoI GCNNNNNNNGC 5 cut(s) 341, 371, 617, 1019, 1161
NarI GGCGCC 1 cut(s) 15
NciI CCSGG 4 cut(s) 12, 267, 382, 383
NcoI CCATGG 1 cut(s) 312
NdeI CATATG 1 cut(s) 1054
NdeII GATC 6 cut(s) 210, 307, 355, 1049, 1228, 1250
NlaIII CATG 9 cut(s) 26, 149, 316, 529, 626, 682, 716, 862, 1150
NlaIV GGNNCC 6 cut(s) 16, 87, 264, 334, 899, 1252
NmeAIII GCCGAG 1 cut(s) 566
NmuCI GTSAC 1 cut(s) 1026
NspI RCATGY 2 cut(s) 716, 1150
PaeI GCATGC 1 cut(s) 716
PagI TCATGA 2 cut(s) 22, 145
PceI AGGCCT 1 cut(s) 758
PciI ACATGT 1 cut(s) 1146
PcsI WCGNNNNNNNCGW 1 cut(s) 817
PdmI GAANNNNTTC 1 cut(s) 1238
PfeI GAWTC 4 cut(s) 26, 173, 386, 600
PkrI GCNGC 8 cut(s) 346, 364, 376, 421, 520, 684, 1081, 1139
PleI GAGTC 1 cut(s) 177
PluTI GGCGCC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 177
Ppu21I YACGTR 1 cut(s) 1031
PscI ACATGT 1 cut(s) 1146
PshBI ATTAAT 1 cut(s) 593
Psp6I CCWGG 3 cut(s) 81, 349, 1104
PspGI CCWGG 3 cut(s) 81, 349, 1104
PspN4I GGNNCC 6 cut(s) 16, 87, 264, 334, 899, 1252
PspPI GGNCC 3 cut(s) 85, 619, 1209
PsuI RGATCY 2 cut(s) 1228, 1250
RsaI GTAC 2 cut(s) 791, 899
RsaNI GTAC 2 cut(s) 790, 898
RseI CAYNNNNRTG 1 cut(s) 731
SaqAI TTAA 2 cut(s) 593, 674
SatI GCNGC 8 cut(s) 345, 363, 375, 420, 519, 683, 1080, 1138
Sau3AI GATC 6 cut(s) 210, 307, 355, 1049, 1228, 1250
Sau96I GGNCC 3 cut(s) 85, 619, 1209
SchI GAGTC 1 cut(s) 177
ScrFI CCNGG 7 cut(s) 12, 83, 267, 351, 382, 383, 1106
SduI GDGCHC 1 cut(s) 1044
SfaNI GCATC 2 cut(s) 223, 1104
SfcI CTRYAG 2 cut(s) 606, 1062
SfoI GGCGCC 1 cut(s) 16
SmaI CCCGGG 1 cut(s) 383
SmiMI CAYNNNNRTG 1 cut(s) 731
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
SphI GCATGC 1 cut(s) 716
Sse9I AATT 5 cut(s) 438, 594, 814, 833, 1125
SseBI AGGCCT 1 cut(s) 758
SsiI CCGC 2 cut(s) 375, 478
SspDI GGCGCC 1 cut(s) 14
SspI AATATT 1 cut(s) 854
SspMI CTAG 2 cut(s) 129, 141
StuI AGGCCT 1 cut(s) 758
StyD4I CCNGG 7 cut(s) 10, 81, 265, 349, 380, 381, 1104
StyI CCWWGG 2 cut(s) 312, 1212
TaaI ACNGT 2 cut(s) 803, 902
TaiI ACGT 2 cut(s) 1033, 1196
TaqI TCGA 1 cut(s) 124
TasI AATT 5 cut(s) 438, 594, 814, 833, 1125
TatI WGTACW 1 cut(s) 789
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 4 cut(s) 26, 173, 386, 600
Tru1I TTAA 2 cut(s) 593, 674
Tru9I TTAA 2 cut(s) 593, 674
TseFI GTSAC 1 cut(s) 1026
TseI GCWGC 7 cut(s) 344, 362, 419, 518, 682, 1079, 1137
Tsp45I GTSAC 1 cut(s) 1026
TspDTI ATGAA 2 cut(s) 18, 847
TspGWI ACGGA 1 cut(s) 1062
TspMI CCCGGG 1 cut(s) 381
VneI GTGCAC 1 cut(s) 1040
VspI ATTAAT 1 cut(s) 593
XapI RAATTY 1 cut(s) 438
XceI RCATGY 2 cut(s) 716, 1150
XmaI CCCGGG 1 cut(s) 381
XmnI GAANNNNTTC 1 cut(s) 1238
XspI CTAG 2 cut(s) 129, 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.