MD04G1178100.v1.1

RING finger and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003923
Physical Location & Seq
Forward (+)
26911973 .. 26916508
4536 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1178100.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGGCTGGGGCGGCCTCTGTAGCATTGGGAACGGAGGAGGTGGGTCAGACAGATGGCAGAACGGATCGTAGTTCGGGAGAGGGAATTCCCCAATCGTTAAATGGAAGTGCTGCTGGAGAAGGAGGTGATGGAGCTGGGGCTAATGGCAGGGATTCTTCTTACCAGAGATATGACATTCAGCAGGCTGCTAGGGGGATTGAACAAGTCCTGCCCTTTTCCTTGCTTCTATTGGTGGTCTTCATCCGCCAGCATTTGCAAGGTTTCTTTGTTACAATTTGGATTGCTGTCGTTATGTTCAAGTCAAATGATATATTAAAGAAACAGACAGCTTTGAAGGGAGAGAGGAAAATTGCAGTTCTAATAGGCATTTCTCTTGCATTCGTGTTGCATGTGGTTGGTGTTTACTGGTGGTACCAGAATGACAATCTTTTGCATCCATTAATTATGCTTCCTCCTAAAGTTATACCACCCTTCTGGCATGCTGTTTTCATCATCATGGTGAATGACACCTTGGTCCGGCAGGCAGCAATGGTGCTGAAGTGTTTTCTATTAATGTACTACAAAAACAGTAGAGGCCGAAACTATCGTAAGCAGGGTCAAATGCTTACTCTGGTTGAGTATCTACTGCTCTTGTACCGTGCCTTGTTGCCAACACCAGTGTGGTATCGGTTCTTCTTGAACAAAGAATATGGGAGTCTCTTTTCGTCACTAATGACAGGGTTATATTTGACTTTCAAGCTCACATCTGTTGTTGAGAAGGTACAATCCTTCTTTGCTGCATTGAAGGCATTGTCACGTAAGGAGGTGCACTATGGGGCATATGCAACATCAGAACAGGTCAATGCAGCTGGCGATTTATGTGCTATTTGCCAGGAGAAGATGCATGCTCCTATCCTACTGCGATGTAAACACATCTTTTGTGAGGACTGCGTATCAGAGTGGTTTGAGAGGGAGCGGACGTGCCCGTTATGCAGAGCTTTGGTTAAACCCGCAGATATCAGATCATTCGGCGATGGATCAACGAGTTTGTTTTTCCAGCTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3 zf-RING_2

Protein Analysis

349

Amino Acids

39.12

Weight (kDa)

9.02

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 284 - 325 3.9e-06 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 285 - 328 4.1e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 286 - 325 1.3e-10 Ring finger domain
zf-C3HC4 PF00097 287 - 324 2.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 287 - 324 4.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 287 - 322 5.3e-08 RING-type zinc-finger
zf-RING_5 PF14634 287 - 325 1.8e-07 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 512
Acc65I GGTACC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 411
AccBSI CCGCTC 1 cut(s) 955
AciI CCGC 4 cut(s) 11, 244, 955, 990
AclWI GGATC 2 cut(s) 72, 1024
AcsI RAATTY 1 cut(s) 84
AcuI CTGAAG 1 cut(s) 557
AfaI GTAC 4 cut(s) 413, 557, 635, 762
AfiI CCNNNNNNNGG 1 cut(s) 516
AgsI TTSAA 6 cut(s) 200, 298, 334, 679, 736, 784
AjiI CACGTC 1 cut(s) 960
AjnI CCWGG 1 cut(s) 870
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AleI CACNNNNGTG 1 cut(s) 658
AluBI AGCT 6 cut(s) 134, 329, 739, 848, 977, 1039
AluI AGCT 6 cut(s) 134, 329, 739, 848, 977, 1039
Alw21I GWGCWC 1 cut(s) 810
Alw26I GTCTC 1 cut(s) 701
Alw44I GTGCAC 1 cut(s) 806
AlwI GGATC 2 cut(s) 72, 1024
AoxI GGCC 2 cut(s) 12, 574
ApaLI GTGCAC 1 cut(s) 806
ApeKI GCWGC 5 cut(s) 110, 185, 524, 776, 845
ApoI RAATTY 1 cut(s) 84
AseI ATTAAT 2 cut(s) 440, 551
Asp718I GGTACC 1 cut(s) 411
AspS9I GGNCC 1 cut(s) 514
AsuHPI GGTGA 2 cut(s) 137, 511
AvaII GGWCC 1 cut(s) 514
BaeGI GKGCMC 2 cut(s) 810, 965
BanI GGYRCC 1 cut(s) 411
BarI GAAGNNNNNNTAC 2 cut(s) 656, 688
BbsI GAAGAC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 810
BbvI GCAGC 5 cut(s) 97, 172, 536, 763, 857
BccI CCATC 3 cut(s) 47, 122, 1007
BcgI CGANNNNNNTGC 2 cut(s) 842, 876
BciT130I CCWGG 1 cut(s) 872
BcoDI GTCTC 1 cut(s) 701
BfaI CTAG 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 18
BisI GCNGC 6 cut(s) 12, 111, 186, 525, 777, 846
BlsI GCNGC 6 cut(s) 13, 112, 187, 526, 778, 847
Bme1390I CCNGG 1 cut(s) 872
Bme18I GGWCC 1 cut(s) 514
BmgBI CACGTC 1 cut(s) 960
BmgT120I GGNCC 1 cut(s) 514
BmiI GGNNCC 1 cut(s) 413
BmrFI CCNGG 1 cut(s) 872
BmsI GCATC 2 cut(s) 442, 870
BpiI GAAGAC 1 cut(s) 229
BpmI CTGGAG 1 cut(s) 135
BsaAI YACGTR 1 cut(s) 797
BsaJI CCNNGG 1 cut(s) 510
BsaXI ACNNNNNCTCC 2 cut(s) 944, 974
Bsc4I CCNNNNNNNGG 1 cut(s) 516
Bse1I ACTGG 2 cut(s) 410, 656
Bse3DI GCAATG 1 cut(s) 534
BseBI CCWGG 1 cut(s) 872
BseDI CCNNGG 1 cut(s) 510
BseGI GGATG 2 cut(s) 240, 433
BseLI CCNNNNNNNGG 1 cut(s) 516
BseMI GCAATG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 410, 656
BseRI GAGGAG 1 cut(s) 50
BseSI GKGCMC 2 cut(s) 810, 965
BseXI GCAGC 5 cut(s) 97, 172, 536, 763, 857
BseYI CCCAGC 2 cut(s) 5, 134
BshFI GGCC 2 cut(s) 14, 576
BshNI GGYRCC 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 810
BsiSI CCGG 1 cut(s) 517
BslI CCNNNNNNNGG 1 cut(s) 516
BsmAI GTCTC 1 cut(s) 701
BsmI GAATGC 1 cut(s) 377
BsnI GGCC 2 cut(s) 14, 576
Bsp1286I GDGCHC 2 cut(s) 810, 965
Bsp143I GATC 3 cut(s) 64, 1001, 1016
BspACI CCGC 4 cut(s) 11, 244, 955, 990
BspANI GGCC 2 cut(s) 14, 576
BspLI GGNNCC 1 cut(s) 413
BspPI GGATC 2 cut(s) 72, 1024
BspT107I GGYRCC 1 cut(s) 411
BsrBI CCGCTC 1 cut(s) 955
BsrDI GCAATG 1 cut(s) 534
BsrI ACTGG 2 cut(s) 410, 656
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 3 cut(s) 64, 1001, 1016
BssT1I CCWWGG 1 cut(s) 510
Bst2UI CCWGG 1 cut(s) 872
Bst4CI ACNGT 2 cut(s) 569, 638
BstBAI YACGTR 1 cut(s) 797
BstC8I GCNNGC 6 cut(s) 183, 248, 480, 522, 850, 885
BstF5I GGATG 2 cut(s) 240, 433
BstKTI GATC 3 cut(s) 67, 1004, 1019
BstMAI GTCTC 1 cut(s) 701
BstMBI GATC 3 cut(s) 64, 1001, 1016
BstMWI GCNNNNNNNGC 4 cut(s) 11, 20, 785, 969
BstNI CCWGG 1 cut(s) 872
BstNSI RCATGY 3 cut(s) 392, 482, 887
BstSCI CCNGG 1 cut(s) 870
BstSFI CTRYAG 1 cut(s) 18
BstSLI GKGCMC 2 cut(s) 810, 965
BstV1I GCAGC 5 cut(s) 97, 172, 536, 763, 857
BstV2I GAAGAC 1 cut(s) 229
BstXI CCANNNNNNTGG 1 cut(s) 474
BsuRI GGCC 2 cut(s) 14, 576
BtgZI GCGATG 2 cut(s) 916, 1026
BtrI CACGTC 1 cut(s) 960
BtsCI GGATG 2 cut(s) 240, 433
BtsIMutI CAGTG 1 cut(s) 663
Cac8I GCNNGC 6 cut(s) 183, 248, 480, 522, 850, 885
Cfr13I GGNCC 1 cut(s) 514
Csp6I GTAC 4 cut(s) 412, 556, 634, 761
CviAII CATG 4 cut(s) 389, 479, 496, 884
CviQI GTAC 4 cut(s) 412, 556, 634, 761
DpnI GATC 3 cut(s) 66, 1003, 1018
DpnII GATC 3 cut(s) 64, 1001, 1016
DrdI GACNNNNNNGTC 1 cut(s) 512
DseDI GACNNNNNNGTC 1 cut(s) 512
EciI GGCGGA 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 510
Eco32I GATATC 1 cut(s) 997
Eco47I GGWCC 1 cut(s) 514
Eco57I CTGAAG 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 870
EcoRV GATATC 1 cut(s) 997
EcoT14I CCWWGG 1 cut(s) 510
EcoT22I ATGCAT 1 cut(s) 885
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 4 cut(s) 392, 482, 499, 887
FatI CATG 4 cut(s) 388, 478, 495, 883
FauI CCCGC 1 cut(s) 997
FauNDI CATATG 1 cut(s) 820
Fnu4HI GCNGC 6 cut(s) 12, 111, 186, 525, 777, 846
FokI GGATG 2 cut(s) 227, 420
Fsp4HI GCNGC 6 cut(s) 12, 111, 186, 525, 777, 846
FspBI CTAG 1 cut(s) 189
GluI GCNGC 6 cut(s) 12, 111, 186, 525, 777, 846
GsaI CCCAGC 2 cut(s) 9, 138
GsuI CTGGAG 1 cut(s) 135
HaeIII GGCC 2 cut(s) 14, 576
HapII CCGG 1 cut(s) 517
Hin1II CATG 4 cut(s) 392, 482, 499, 887
HinfI GANTC 2 cut(s) 152, 694
HpaII CCGG 1 cut(s) 517
HphI GGTGA 2 cut(s) 137, 511
Hpy166II GTNNAC 3 cut(s) 403, 808, 908
Hpy188I TCNGA 5 cut(s) 48, 832, 937, 1001, 1046
Hpy188III TCNNGA 2 cut(s) 75, 676
Hpy8I GTNNAC 3 cut(s) 403, 808, 908
HpyAV CCTTC 6 cut(s) 113, 328, 481, 751, 778, 778
HpyCH4III ACNGT 2 cut(s) 569, 638
HpyCH4IV ACGT 2 cut(s) 796, 959
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 20, 785, 969
HpySE526I ACGT 2 cut(s) 796, 959
Hsp92II CATG 4 cut(s) 392, 482, 499, 887
KpnI GGTACC 1 cut(s) 415
Kzo9I GATC 3 cut(s) 64, 1001, 1016
LmnI GCTCC 3 cut(s) 131, 892, 952
Lsp1109I GCAGC 5 cut(s) 97, 172, 536, 763, 857
LweI GCATC 2 cut(s) 442, 870
MaeI CTAG 1 cut(s) 189
MaeII ACGT 2 cut(s) 796, 959
MaeIII GTNAC 3 cut(s) 268, 705, 792
MalI GATC 3 cut(s) 66, 1003, 1018
MbiI CCGCTC 1 cut(s) 955
MboI GATC 3 cut(s) 64, 1001, 1016
MboII GAAGA 4 cut(s) 147, 229, 664, 889
MhlI GDGCHC 2 cut(s) 810, 965
MluCI AATT 4 cut(s) 84, 273, 348, 441
MlyI GAGTC 1 cut(s) 703
Mph1103I ATGCAT 1 cut(s) 885
MseI TTAA 5 cut(s) 98, 314, 440, 551, 984
MslI CAYNNNNRTG 3 cut(s) 494, 497, 658
MspA1I CMGCKG 2 cut(s) 848, 1039
MspI CCGG 1 cut(s) 517
MspR9I CCNGG 1 cut(s) 872
Mva1269I GAATGC 1 cut(s) 377
MvaI CCWGG 1 cut(s) 872
MwoI GCNNNNNNNGC 4 cut(s) 11, 20, 785, 969
NdeI CATATG 1 cut(s) 820
NdeII GATC 3 cut(s) 64, 1001, 1016
NlaIII CATG 4 cut(s) 392, 482, 499, 887
NlaIV GGNNCC 1 cut(s) 413
NmuCI GTSAC 2 cut(s) 705, 792
NsiI ATGCAT 1 cut(s) 885
NspI RCATGY 3 cut(s) 392, 482, 887
OliI CACNNNNGTG 1 cut(s) 658
PaeI GCATGC 2 cut(s) 482, 887
PctI GAATGC 1 cut(s) 377
PfeI GAWTC 1 cut(s) 152
PkrI GCNGC 6 cut(s) 13, 112, 187, 526, 778, 847
PleI GAGTC 1 cut(s) 702
PpsI GAGTC 1 cut(s) 702
Ppu21I YACGTR 1 cut(s) 797
PshBI ATTAAT 2 cut(s) 440, 551
Psp6I CCWGG 1 cut(s) 870
PspFI CCCAGC 2 cut(s) 5, 134
PspGI CCWGG 1 cut(s) 870
PspN4I GGNNCC 1 cut(s) 413
PspPI GGNCC 1 cut(s) 514
PvuII CAGCTG 2 cut(s) 848, 1039
RsaI GTAC 4 cut(s) 413, 557, 635, 762
RsaNI GTAC 4 cut(s) 412, 556, 634, 761
RseI CAYNNNNRTG 3 cut(s) 494, 497, 658
SaqAI TTAA 5 cut(s) 98, 314, 440, 551, 984
SatI GCNGC 6 cut(s) 12, 111, 186, 525, 777, 846
Sau3AI GATC 3 cut(s) 64, 1001, 1016
Sau96I GGNCC 1 cut(s) 514
SchI GAGTC 1 cut(s) 703
ScrFI CCNGG 1 cut(s) 872
SduI GDGCHC 2 cut(s) 810, 965
SfaNI GCATC 2 cut(s) 442, 870
SfcI CTRYAG 1 cut(s) 18
SinI GGWCC 1 cut(s) 514
SmiMI CAYNNNNRTG 3 cut(s) 494, 497, 658
SphI GCATGC 2 cut(s) 482, 887
Sse9I AATT 4 cut(s) 84, 273, 348, 441
SsiI CCGC 4 cut(s) 11, 244, 955, 990
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 870
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 2 cut(s) 569, 638
TaiI ACGT 2 cut(s) 799, 962
TasI AATT 4 cut(s) 84, 273, 348, 441
TatI WGTACW 1 cut(s) 555
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 1 cut(s) 152
Tru1I TTAA 5 cut(s) 98, 314, 440, 551, 984
Tru9I TTAA 5 cut(s) 98, 314, 440, 551, 984
TscAI CASTG 1 cut(s) 663
TseFI GTSAC 2 cut(s) 705, 792
TseI GCWGC 5 cut(s) 110, 185, 524, 776, 845
Tsp45I GTSAC 2 cut(s) 705, 792
TspDTI ATGAA 2 cut(s) 229, 478
TspGWI ACGGA 2 cut(s) 47, 77
TspRI CASTG 1 cut(s) 663
VneI GTGCAC 1 cut(s) 806
VpaK11BI GGWCC 1 cut(s) 514
VspI ATTAAT 2 cut(s) 440, 551
XapI RAATTY 1 cut(s) 84
XceI RCATGY 3 cut(s) 392, 482, 887
XcmI CCANNNNNNNNNTGG 2 cut(s) 98, 657
XspI CTAG 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.