MD01G1171400.v1.1

RING finger and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003923
Physical Location & Seq
Forward (+)
27408142 .. 27412313
4172 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1171400.v1.1.491

Sequence Viewer

Length: 1407 bp
ATGGAGGTTTCTTCCGGAAGTAGTAGTCCCGCCAGTGGGGGAGCCACTGGGAATAACAGCAACAGTAATAGCAATACGTCGTCGTTCGGGTCTATGCAATTCCCCGCCCTCCGGTTCTTCCAGTCGCCGCTCTCATTCGTATTGGATTACTCCGGAATACTCTCTCCCAGCTCTTCCCGCCGCGATTCCGATGTTCCGGTGGTCAACGCTGGAGTCGTTGCCGATTCTCGGCCACACTCCCCTACGGCCTGCACCTCCGGGTCGAGCAACACCAGGGAGGTTTCAATTCGGATTATCGGCGCCGGCGAGCATGTGGACCGTGGGAACGGGGGTGCGACGGTGCCGGTTCAAGGGGAGGAGAATGGTGGTTCGCTGGGTAGGGTTCAATCGTTGGATAATCAGGTGGGTATGGTCAATGGGGTCGGGTCGGACGAGAGAGTCCCCTTGGTGGCTCCGTCGTCTCCGGAACACGCTGGGGATACTGGGAATGGCGCGGAGGATAGTTCTAGGGATTCTGCACACCAGAGATATGATATTCAGCAGATAGCTAGGTGGATTGAGCAGATTCTTCCGTTTTCGTTGCTGTTGCTCGTCGTCTTCATTCGTCAGCATTTGCAAGGTTTCTTTGTCACTATATGGGTATCAGCTGTCATGTATAGGTCAAATGATGTTGTTAAGAAGCAGACTGCTCTAAAGGGAGATAGAAAAATCTCAGTTCTTGCAAGTATTGCCGGTGCTTTTATGCTTCATGTAGTAGGTGTTTACTGGTGGTATCGAAGTGACGATCTTCTATTCCCATTGGCAATGCTTCCTCCAAAAGCAATACCACCTTTCTGGCATGCCATATTCACCATTTTGGTAAATGATACCATGGTGAGGCAGGCAGGGATGGCTTTCAAGTGTTTGTTGCTTATATACTACAAGAATGGCAGAGGCCATAATTATCGTCGGCAGGGTCAAATGTTAACTGTAGTTGAGTATACACTGCTGCTGTACCGGGCTTTGTTACCAACACCTGTTTGGTACAGATTTTTCCTTAACAAAGACAATGGGAGCCTCTTCTCCTCACTTACAACGGGATTATATTTAACTTTTAAGCTAACATCTGTTGTTGAGAAGGTCCAATCCTTTTTTGCTTCCATAAGAGCATTGTCACGAAAGGATATCCATTATGGGTCTCATGCAACATCAGAACAGGTAAACGCAGCGGGAGACCTTTGTGCCATTTGCCAGGAGAAGATACATGCTCCGATTCTGTTGCGCTGTAAACATATTTTCTGCGAGGATTGTGTCTCTGAATGGTTTGAGAGAGAAAGAACTTGCCCGCTATGCAGGTCAGTAGTAAAACCTGCGGATTTGCGAACATTTGGGGATGGATCAACGAGTTTATTTTTCCAGTTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

469

Amino Acids

51.51

Weight (kDa)

8.51

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 405 - 448 4.5e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 406 - 445 3.3e-10 Ring finger domain
zf-C3HC4 PF00097 407 - 444 4.2e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 407 - 444 3.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 407 - 445 2.2e-07 zinc-RING finger domain
zf-RING_UBOX PF13445 407 - 442 6.6e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 437
Acc36I ACCTGC 2 cut(s) 1323, 1357
AccB1I GGYRCC 2 cut(s) 299, 340
AccBSI CCGCTC 1 cut(s) 130
AccI GTMKAC 1 cut(s) 980
AccII CGCG 2 cut(s) 183, 494
AccIII TCCGGA 3 cut(s) 14, 152, 463
AciI CCGC 9 cut(s) 30, 105, 128, 178, 181, 494, 1208, 1325, 1352
AclWI GGATC 1 cut(s) 1384
AcoI YGGCCR 1 cut(s) 230
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 2 cut(s) 995, 1025
AfiI CCNNNNNNNGG 7 cut(s) 35, 36, 111, 228, 350, 448, 876
AgsI TTSAA 4 cut(s) 285, 350, 386, 898
AjnI CCWGG 2 cut(s) 272, 1230
AluBI AGCT 4 cut(s) 171, 548, 647, 1099
AluI AGCT 4 cut(s) 171, 548, 647, 1099
Alw26I GTCTC 4 cut(s) 465, 1182, 1206, 1297
AlwI GGATC 1 cut(s) 1384
Aor13HI TCCGGA 3 cut(s) 14, 152, 463
AoxI GGCC 3 cut(s) 230, 246, 934
ApeKI GCWGC 2 cut(s) 988, 1205
AspLEI GCGC 3 cut(s) 302, 494, 1263
AspS9I GGNCC 2 cut(s) 316, 1120
AsuC2I CCSGG 2 cut(s) 259, 998
AsuHPI GGTGA 2 cut(s) 841, 886
AvaII GGWCC 2 cut(s) 316, 1120
BaeI ACNNNNGTAYC 2 cut(s) 858, 891
BanI GGYRCC 2 cut(s) 299, 340
BbsI GAAGAC 1 cut(s) 589
BbvI GCAGC 2 cut(s) 975, 1217
BccI CCATC 2 cut(s) 883, 1367
BceAI ACGGC 1 cut(s) 261
BcgI CGANNNNNNTGC 2 cut(s) 1240, 1274
BciT130I CCWGG 2 cut(s) 274, 1232
BciVI GTATCC 1 cut(s) 472
BcnI CCSGG 2 cut(s) 259, 998
BcoDI GTCTC 4 cut(s) 465, 1182, 1206, 1297
BfaI CTAG 2 cut(s) 507, 549
BfmI CTRYAG 1 cut(s) 969
BfoI RGCGCY 1 cut(s) 303
BfuAI ACCTGC 2 cut(s) 1323, 1357
BfuI GTATCC 1 cut(s) 472
BisI GCNGC 4 cut(s) 128, 181, 989, 1206
BlsI GCNGC 4 cut(s) 129, 182, 990, 1207
Bme1390I CCNGG 4 cut(s) 259, 274, 998, 1232
Bme18I GGWCC 2 cut(s) 316, 1120
BmgT120I GGNCC 2 cut(s) 316, 1120
BmiI GGNNCC 5 cut(s) 43, 301, 342, 453, 1055
BmrFI CCNGG 4 cut(s) 259, 274, 998, 1232
BmrI ACTGGG 2 cut(s) 57, 492
BmuI ACTGGG 2 cut(s) 57, 492
BpiI GAAGAC 1 cut(s) 589
BpmI CTGGAG 1 cut(s) 231
BpuMI CCSGG 2 cut(s) 259, 998
BsaHI GRCGYC 1 cut(s) 300
BsaI GGTCTC 2 cut(s) 1182, 1206
BsaJI CCNNGG 4 cut(s) 273, 319, 444, 870
BsaWI WCCGGW 5 cut(s) 14, 111, 152, 196, 463
Bsc4I CCNNNNNNNGG 7 cut(s) 35, 36, 111, 228, 350, 448, 876
Bse118I RCCGGY 3 cut(s) 302, 343, 731
Bse1I ACTGG 6 cut(s) 33, 52, 121, 487, 770, 1396
Bse3DI GCAATG 1 cut(s) 810
BseAI TCCGGA 3 cut(s) 14, 152, 463
BseBI CCWGG 2 cut(s) 274, 1232
BseDI CCNNGG 4 cut(s) 273, 319, 444, 870
BseGI GGATG 2 cut(s) 894, 1378
BseLI CCNNNNNNNGG 7 cut(s) 35, 36, 111, 228, 350, 448, 876
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 1 cut(s) 726
BseNI ACTGG 6 cut(s) 33, 52, 121, 487, 770, 1396
BseRI GAGGAG 2 cut(s) 371, 1054
BseXI GCAGC 2 cut(s) 975, 1217
BseYI CCCAGC 3 cut(s) 167, 373, 473
BsgI GTGCAG 2 cut(s) 235, 501
Bsh1236I CGCG 2 cut(s) 183, 494
BshFI GGCC 3 cut(s) 232, 248, 936
BshNI GGYRCC 2 cut(s) 299, 340
BslFI GGGAC 2 cut(s) 12, 425
BslI CCNNNNNNNGG 7 cut(s) 35, 36, 111, 228, 350, 448, 876
BsmAI GTCTC 4 cut(s) 465, 1182, 1206, 1297
BsmBI CGTCTC 1 cut(s) 465
BsmFI GGGAC 2 cut(s) 12, 425
BsnI GGCC 3 cut(s) 232, 248, 936
Bso31I GGTCTC 2 cut(s) 1182, 1206
Bsp13I TCCGGA 3 cut(s) 14, 152, 463
Bsp143I GATC 2 cut(s) 784, 1376
Bsp19I CCATGG 1 cut(s) 870
BspACI CCGC 9 cut(s) 30, 105, 128, 178, 181, 494, 1208, 1325, 1352
BspANI GGCC 3 cut(s) 232, 248, 936
BspCNI CTCAG 1 cut(s) 725
BspEI TCCGGA 3 cut(s) 14, 152, 463
BspFNI CGCG 2 cut(s) 183, 494
BspLI GGNNCC 5 cut(s) 43, 301, 342, 453, 1055
BspMI ACCTGC 2 cut(s) 1323, 1357
BspPI GGATC 1 cut(s) 1384
BspQI GCTCTTC 1 cut(s) 178
BspT107I GGYRCC 2 cut(s) 299, 340
BspTNI GGTCTC 2 cut(s) 1182, 1206
BsrBI CCGCTC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 810
BsrFI RCCGGY 3 cut(s) 302, 343, 731
BsrI ACTGG 6 cut(s) 33, 52, 121, 487, 770, 1396
BssAI RCCGGY 3 cut(s) 302, 343, 731
BssECI CCNNGG 4 cut(s) 273, 319, 444, 870
BssMI GATC 2 cut(s) 784, 1376
BssNAI GTATAC 1 cut(s) 981
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 2 cut(s) 444, 870
Bst1107I GTATAC 1 cut(s) 981
Bst2UI CCWGG 2 cut(s) 274, 1232
Bst4CI ACNGT 4 cut(s) 65, 320, 340, 970
Bst6I CTCTTC 2 cut(s) 178, 1064
BstACI GRCGYC 1 cut(s) 300
BstAPI GCANNNNNTGC 1 cut(s) 728
BstC8I GCNNGC 6 cut(s) 250, 304, 308, 840, 882, 1325
BstDEI CTNAG 1 cut(s) 712
BstDSI CCRYGG 2 cut(s) 319, 870
BstF5I GGATG 2 cut(s) 894, 1378
BstFNI CGCG 2 cut(s) 183, 494
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 3 cut(s) 302, 494, 1263
BstKTI GATC 2 cut(s) 787, 1379
BstMAI GTCTC 4 cut(s) 465, 1182, 1206, 1297
BstMBI GATC 2 cut(s) 784, 1376
BstMWI GCNNNNNNNGC 4 cut(s) 177, 728, 890, 1329
BstNI CCWGG 2 cut(s) 274, 1232
BstNSI RCATGY 3 cut(s) 314, 842, 1247
BstSCI CCNGG 4 cut(s) 257, 272, 996, 1230
BstSFI CTRYAG 1 cut(s) 969
BstUI CGCG 2 cut(s) 183, 494
BstV1I GCAGC 2 cut(s) 975, 1217
BstV2I GAAGAC 1 cut(s) 589
BstXI CCANNNNNNTGG 1 cut(s) 834
BstZ17I GTATAC 1 cut(s) 981
BsuI GTATCC 1 cut(s) 472
BsuRI GGCC 3 cut(s) 232, 248, 936
BtgI CCRYGG 2 cut(s) 319, 870
BtsCI GGATG 2 cut(s) 894, 1378
BtsI GCAGTG 1 cut(s) 983
BtsIMutI CAGTG 3 cut(s) 40, 45, 983
BveI ACCTGC 2 cut(s) 1323, 1357
Cac8I GCNNGC 6 cut(s) 250, 304, 308, 840, 882, 1325
CfoI GCGC 3 cut(s) 302, 494, 1263
Cfr10I RCCGGY 3 cut(s) 302, 343, 731
Cfr13I GGNCC 2 cut(s) 316, 1120
Csp6I GTAC 2 cut(s) 994, 1024
CviAII CATG 7 cut(s) 311, 652, 749, 839, 871, 1181, 1244
CviQI GTAC 2 cut(s) 994, 1024
DdeI CTNAG 1 cut(s) 712
DinI GGCGCC 1 cut(s) 301
DpnI GATC 2 cut(s) 786, 1378
DpnII GATC 2 cut(s) 784, 1376
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
EaeI YGGCCR 1 cut(s) 230
Eam1104I CTCTTC 2 cut(s) 178, 1064
EarI CTCTTC 2 cut(s) 178, 1064
Eco130I CCWWGG 2 cut(s) 444, 870
Eco31I GGTCTC 2 cut(s) 1182, 1206
Eco32I GATATC 1 cut(s) 1165
Eco47I GGWCC 2 cut(s) 316, 1120
EcoRII CCWGG 2 cut(s) 272, 1230
EcoRV GATATC 1 cut(s) 1165
EcoT14I CCWWGG 2 cut(s) 444, 870
EgeI GGCGCC 1 cut(s) 301
EheI GGCGCC 1 cut(s) 301
ErhI CCWWGG 2 cut(s) 444, 870
Esp3I CGTCTC 1 cut(s) 465
FaeI CATG 7 cut(s) 314, 655, 752, 842, 874, 1184, 1247
FaqI GGGAC 2 cut(s) 12, 425
FatI CATG 7 cut(s) 310, 651, 748, 838, 870, 1180, 1243
FauI CCCGC 5 cut(s) 37, 112, 185, 1201, 1332
FblI GTMKAC 1 cut(s) 980
Fnu4HI GCNGC 4 cut(s) 128, 181, 989, 1206
FokI GGATG 2 cut(s) 901, 1385
Fsp4HI GCNGC 4 cut(s) 128, 181, 989, 1206
FspBI CTAG 2 cut(s) 507, 549
GlaI GCGC 3 cut(s) 301, 493, 1262
GluI GCNGC 4 cut(s) 128, 181, 989, 1206
GsaI CCCAGC 3 cut(s) 171, 377, 477
GsuI CTGGAG 1 cut(s) 231
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 3 cut(s) 232, 248, 936
HhaI GCGC 3 cut(s) 302, 494, 1263
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 7 cut(s) 314, 655, 752, 842, 874, 1184, 1247
Hin6I GCGC 3 cut(s) 300, 492, 1261
HinP1I GCGC 3 cut(s) 300, 492, 1261
HincII GTYRAC 2 cut(s) 205, 966
HindII GTYRAC 2 cut(s) 205, 966
HinfI GANTC 7 cut(s) 185, 213, 224, 438, 512, 565, 1252
HpaI GTTAAC 1 cut(s) 966
HphI GGTGA 2 cut(s) 841, 886
Hpy166II GTNNAC 7 cut(s) 205, 316, 763, 966, 981, 1201, 1268
Hpy188I TCNGA 6 cut(s) 190, 291, 430, 1192, 1251, 1297
Hpy188III TCNNGA 4 cut(s) 15, 153, 464, 1155
Hpy8I GTNNAC 7 cut(s) 205, 316, 763, 966, 981, 1201, 1268
Hpy99I CGWCG 6 cut(s) 82, 85, 340, 460, 596, 951
HpyAV CCTTC 1 cut(s) 1111
HpyCH4III ACNGT 4 cut(s) 65, 320, 340, 970
HpyCH4IV ACGT 1 cut(s) 77
HpyCH4V TGCA 7 cut(s) 97, 252, 518, 616, 722, 1184, 1332
HpyF10VI GCNNNNNNNGC 4 cut(s) 177, 728, 890, 1329
HpyF3I CTNAG 1 cut(s) 712
HpySE526I ACGT 1 cut(s) 77
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 7 cut(s) 314, 655, 752, 842, 874, 1184, 1247
HspAI GCGC 3 cut(s) 300, 492, 1261
KasI GGCGCC 1 cut(s) 299
Kpn2I TCCGGA 3 cut(s) 14, 152, 463
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
KspAI GTTAAC 1 cut(s) 966
Kzo9I GATC 2 cut(s) 784, 1376
LguI GCTCTTC 1 cut(s) 178
LmnI GCTCC 4 cut(s) 41, 457, 1053, 1252
Lsp1109I GCAGC 2 cut(s) 975, 1217
MaeI CTAG 2 cut(s) 507, 549
MaeII ACGT 1 cut(s) 77
MaeIII GTNAC 4 cut(s) 628, 779, 1005, 1152
MalI GATC 2 cut(s) 786, 1378
MbiI CCGCTC 1 cut(s) 130
MboI GATC 2 cut(s) 784, 1376
MboII GAAGA 8 cut(s) 3, 109, 165, 560, 589, 779, 1051, 1249
MluCI AATT 3 cut(s) 98, 285, 940
Mly113I GGCGCC 1 cut(s) 300
MlyI GAGTC 2 cut(s) 222, 447
MmeI TCCRAC 2 cut(s) 372, 408
MreI CGCCGGCG 1 cut(s) 302
MroI TCCGGA 3 cut(s) 14, 152, 463
MroNI GCCGGC 1 cut(s) 302
MseI TTAA 5 cut(s) 675, 965, 1038, 1088, 1095
MspA1I CMGCKG 2 cut(s) 647, 1208
MspR9I CCNGG 4 cut(s) 259, 274, 998, 1232
MvaI CCWGG 2 cut(s) 274, 1232
MvnI CGCG 2 cut(s) 183, 494
MwoI GCNNNNNNNGC 4 cut(s) 177, 728, 890, 1329
NaeI GCCGGC 1 cut(s) 304
NarI GGCGCC 1 cut(s) 300
NciI CCSGG 2 cut(s) 259, 998
NcoI CCATGG 1 cut(s) 870
NdeII GATC 2 cut(s) 784, 1376
NgoMIV GCCGGC 1 cut(s) 302
NlaIII CATG 7 cut(s) 314, 655, 752, 842, 874, 1184, 1247
NlaIV GGNNCC 5 cut(s) 43, 301, 342, 453, 1055
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 3 cut(s) 628, 779, 1152
NspI RCATGY 3 cut(s) 314, 842, 1247
PaeI GCATGC 1 cut(s) 842
PciSI GCTCTTC 1 cut(s) 178
PcsI WCGNNNNNNNCGW 3 cut(s) 213, 303, 429
PdiI GCCGGC 1 cut(s) 304
PfeI GAWTC 5 cut(s) 185, 224, 512, 565, 1252
PkrI GCNGC 4 cut(s) 129, 182, 990, 1207
PleI GAGTC 2 cut(s) 221, 446
PluTI GGCGCC 1 cut(s) 303
PpsI GAGTC 2 cut(s) 221, 446
Psp6I CCWGG 2 cut(s) 272, 1230
PspFI CCCAGC 3 cut(s) 167, 373, 473
PspGI CCWGG 2 cut(s) 272, 1230
PspN4I GGNNCC 5 cut(s) 43, 301, 342, 453, 1055
PspPI GGNCC 2 cut(s) 316, 1120
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 2 cut(s) 995, 1025
RsaNI GTAC 2 cut(s) 994, 1024
SapI GCTCTTC 1 cut(s) 178
SaqAI TTAA 5 cut(s) 675, 965, 1038, 1088, 1095
SatI GCNGC 4 cut(s) 128, 181, 989, 1206
Sau3AI GATC 2 cut(s) 784, 1376
Sau96I GGNCC 2 cut(s) 316, 1120
SchI GAGTC 2 cut(s) 222, 447
ScrFI CCNGG 4 cut(s) 259, 274, 998, 1232
SfcI CTRYAG 1 cut(s) 969
SfoI GGCGCC 1 cut(s) 301
SgrAI CRCCGGYG 1 cut(s) 302
SinI GGWCC 2 cut(s) 316, 1120
SphI GCATGC 1 cut(s) 842
Sse9I AATT 3 cut(s) 98, 285, 940
SsiI CCGC 9 cut(s) 30, 105, 128, 178, 181, 494, 1208, 1325, 1352
SspDI GGCGCC 1 cut(s) 299
SspMI CTAG 2 cut(s) 507, 549
StyD4I CCNGG 4 cut(s) 257, 272, 996, 1230
StyI CCWWGG 2 cut(s) 444, 870
TaaI ACNGT 4 cut(s) 65, 320, 340, 970
TaiI ACGT 1 cut(s) 80
TaqI TCGA 2 cut(s) 263, 775
TasI AATT 3 cut(s) 98, 285, 940
TauI GCSGC 2 cut(s) 130, 183
TfiI GAWTC 5 cut(s) 185, 224, 512, 565, 1252
Tru1I TTAA 5 cut(s) 675, 965, 1038, 1088, 1095
Tru9I TTAA 5 cut(s) 675, 965, 1038, 1088, 1095
TscAI CASTG 3 cut(s) 40, 52, 990
TseFI GTSAC 3 cut(s) 628, 779, 1152
TseI GCWGC 2 cut(s) 988, 1205
Tsp45I GTSAC 3 cut(s) 628, 779, 1152
TspDTI ATGAA 2 cut(s) 589, 737
TspGWI ACGGA 2 cut(s) 444, 561
TspRI CASTG 3 cut(s) 40, 52, 990
VpaK11BI GGWCC 2 cut(s) 316, 1120
XceI RCATGY 3 cut(s) 314, 842, 1247
XcmI CCANNNNNNNNNTGG 1 cut(s) 1017
XmiI GTMKAC 1 cut(s) 980
XspI CTAG 2 cut(s) 507, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.