Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0003923

Physical Location & Seq

Genomic Coordinates Forward (+)

23120144 .. 23124400

Gene Structure 4257 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g21390.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGGAGGCATCTTCCGGAAATAGTAGTCCCGCCAGTGGGGGAGCCACTGAGGTTGGCAGCAATACGTCGTCGTTCGGGTCTATGCAGTTCCCCGCCCTCCGGTTCTTCCAGTCGCCGCTCTCATTCGTGTTGGATTACTCCGGAATACTCTCTCCCAGCTCTTCCCGCCGCGATTCCGACGCTCCGGTGGTCAACGCTGTAGTCGTTGCCGATTCTCAGCCCCACTCCCCTACGGCCTGCACCTCCGGGTCGAGCAACACCGGGGAGGTTTCGATTCGGATTATCGGCGCCGGCGAGCAAGAGGACCGCGGGAACGGGGGTGCGACGGGGCCGGTTCAAGGGGAGGAGAATGATGGGTCGCCGGGTAGGGTTCAATCGTTGGATGATCTGGCGGGTACAGTCAATGGGGTTGGGTCGGACGAGAGAGTCCCCTTGGTGGCTTCGTCGTCTCCCACACACGCTGGGGATACTGGGAATGGCGCGGAGGATAGTTCTAGGGATTCTGCTTACCAGAGATATGATATTCAGCAGATAGCTAGGTGGATTGAGCAGATTCTTCCGTTTTCGTTGCTCTTACTCGTTGTGTTCATTCGTCAGCATTTGCAAGGTTTCTTTGTCACTATATGGGTATCAGCTGTCATGTATAGGTCAAATGATGTTGTTAAGAAGCAGACTTCTCTAAAGGGGGATAGGAAAATCTCTGTTCTTGTAAGTATTGCTGGTGCTTTTATGCTTCATGTAGTTGTTGTTTACTGGTGGTATCGAAGTGATGATCTTCTATACCCATTGGCAATGCTTCCTCCAAAAACCATACCACCTTTCTGGCATGCCATATTTATCATTTTGGTAAATGATACCATGGTGAGACAGGCAGCGATGGCTTTCAAGTGTTTGTTGCTTATATACTACAAGAATGGCAGAGGCCATAACTATCGTCGGCAGGGTCAAATGTTAACTGTCATTGAGTATACACTGCTGCTGTACCGTGCCTTGTTACCAGCACCCGTTTGGTACAGATTCTTCCTGAACAAAGACAATGGGAGCCTCTTCTCCTCACTTACAACGGGATTATATTTAACTTTCAAGCTAACATCTGTTGTTGAGAAGGTCCAATCCTTTTTGGCTTCCATAAGGGCATTGTCACGGAAGGATATCCATTATGGGTCTCATGCAACATTGGAACAGGTAAATGCAGCAGGAGACCTTTGTGCCATTTGCCAGGAGAAGATGCATGCTCCAATTCTGTTGCGCTGTAAACATATTTTCTGCGAGGATTGTGTCTCTGAATGGTTTGAGAGAGAAAGAACTTGCCCTCTATGCAGGGCACTAGTAAAACCTGCGGATTTGCGAACATATGGAGACGGATCAACGAGTTTAATTTTCCAGTTGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

465

Amino Acids

51.02

Weight (kDa)

6.79

Isoelectric Point (pI)

50.1

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003923)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01960
fragaria_vesca	FvH4_6g09390 FvH4_7g26260 FvH4_7g26260
malus_domestica	MD01G1171400.v1.1 MD04G1178100.v1.1 MD07G1237600.v1.1 MD12G1193700.v1.1
prunus_persica	Prupe.2G266800_v2.0.a1 Prupe.2G266800_v2.0.a1 Prupe.6G298000_v2.0.a1
pyrus_communis	pycom01g18360 pycom04g15710 pycom07g21390 pycom12g17990
rosa_chinensis	RchiOBHm_Chr1g0372711 RchiOBHm_Chr3g0459771
rosa_laevigata	RLG00000025058 RLG00000026877
rosa_multiflora	Rmu_sc0009717.1_g000009 Rmu_ssc0000185.1_g000002
rosa_roxburghii	Rroxscaffold_4G00284730 Rroxscaffold_6G00419970
rosa_rugosa	Rorug01G0373800 Rorug03G0038100
rosa_samantha	Rh1AG382600 Rh1BG346100 Rh1CG359400 Rh1DG377200 Rh3AG096800 Rh3BG099500 Rh3CG101400 Rh3DG100800
rosa_wichuraiana	Rw1G033660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	425
Acc36I	ACCTGC	1 cut(s)	1345
AccB1I	GGYRCC	1 cut(s)	287
AccBSI	CCGCTC	1 cut(s)	118
AccI	GTMKAC	1 cut(s)	968
AccII	CGCG	3 cut(s)	171, 309, 482
AccIII	TCCGGA	2 cut(s)	14, 140
AclWI	GGATC	1 cut(s)	1372
AcyI	GRCGYC	1 cut(s)	288
AfaI	GTAC	3 cut(s)	397, 983, 1013
AfiI	CCNNNNNNNGG	5 cut(s)	35, 36, 99, 338, 436
AgsI	TTSAA	4 cut(s)	338, 374, 886, 1084
AhlI	ACTAGT	1 cut(s)	1327
AjnI	CCWGG	1 cut(s)	1218
AluBI	AGCT	4 cut(s)	159, 536, 635, 1087
AluI	AGCT	4 cut(s)	159, 536, 635, 1087
Alw26I	GTCTC	6 cut(s)	453, 859, 1170, 1194, 1285, 1353
AlwI	GGATC	1 cut(s)	1372
Aor13HI	TCCGGA	2 cut(s)	14, 140
AoxI	GGCC	3 cut(s)	234, 329, 922
ApeKI	GCWGC	4 cut(s)	57, 872, 976, 1193
AspLEI	GCGC	3 cut(s)	290, 482, 1251
AspS9I	GGNCC	3 cut(s)	304, 329, 1108
AsuC2I	CCSGG	3 cut(s)	247, 262, 363
AsuHPI	GGTGA	1 cut(s)	874
AvaII	GGWCC	2 cut(s)	304, 1108
BaeGI	GKGCMC	1 cut(s)	1327
BaeI	ACNNNNGTAYC	2 cut(s)	846, 879
BanI	GGYRCC	1 cut(s)	287
BarI	GAAGNNNNNNTAC	2 cut(s)	1004, 1036
BbvI	GCAGC	4 cut(s)	69, 884, 963, 1205
BccI	CCATC	2 cut(s)	347, 871
BceAI	ACGGC	1 cut(s)	249
BciT130I	CCWGG	1 cut(s)	1220
BciVI	GTATCC	1 cut(s)	460
BcnI	CCSGG	3 cut(s)	247, 262, 363
BcoDI	GTCTC	6 cut(s)	453, 859, 1170, 1194, 1285, 1353
BcuI	ACTAGT	1 cut(s)	1327
BfaI	CTAG	3 cut(s)	495, 537, 1328
BfmI	CTRYAG	1 cut(s)	198
BfoI	RGCGCY	1 cut(s)	291
BfuAI	ACCTGC	1 cut(s)	1345
BfuI	GTATCC	1 cut(s)	460
BisI	GCNGC	6 cut(s)	58, 116, 169, 873, 977, 1194
BlsI	GCNGC	6 cut(s)	59, 117, 170, 874, 978, 1195
Bme1390I	CCNGG	4 cut(s)	247, 262, 363, 1220
Bme18I	GGWCC	2 cut(s)	304, 1108
BmgT120I	GGNCC	3 cut(s)	304, 329, 1108
BmiI	GGNNCC	4 cut(s)	43, 289, 330, 1043
BmrFI	CCNGG	4 cut(s)	247, 262, 363, 1220
BmrI	ACTGGG	1 cut(s)	480
BmsI	GCATC	2 cut(s)	17, 1218
BmuI	ACTGGG	1 cut(s)	480
BpuMI	CCSGG	3 cut(s)	247, 262, 363
BsaHI	GRCGYC	1 cut(s)	288
BsaI	GGTCTC	2 cut(s)	1170, 1194
BsaJI	CCNNGG	4 cut(s)	261, 307, 432, 858
BsaWI	WCCGGW	4 cut(s)	14, 99, 140, 184
Bsc4I	CCNNNNNNNGG	5 cut(s)	35, 36, 99, 338, 436
Bse118I	RCCGGY	2 cut(s)	290, 331
Bse1I	ACTGG	5 cut(s)	33, 109, 475, 758, 1384
Bse3DI	GCAATG	1 cut(s)	798
BseAI	TCCGGA	2 cut(s)	14, 140
BseBI	CCWGG	1 cut(s)	1220
BseDI	CCNNGG	4 cut(s)	261, 307, 432, 858
BseGI	GGATG	1 cut(s)	388
BseLI	CCNNNNNNNGG	5 cut(s)	35, 36, 99, 338, 436
BseMI	GCAATG	1 cut(s)	798
BseMII	CTCAG	2 cut(s)	39, 230
BseNI	ACTGG	5 cut(s)	33, 109, 475, 758, 1384
BseRI	GAGGAG	2 cut(s)	359, 1042
BseSI	GKGCMC	1 cut(s)	1327
BseXI	GCAGC	4 cut(s)	69, 884, 963, 1205
BseYI	CCCAGC	2 cut(s)	155, 461
BsgI	GTGCAG	1 cut(s)	223
Bsh1236I	CGCG	3 cut(s)	171, 309, 482
BshFI	GGCC	3 cut(s)	236, 331, 924
BshNI	GGYRCC	1 cut(s)	287
BsiSI	CCGG	9 cut(s)	15, 100, 141, 185, 246, 261, 291, 332, 362
BslFI	GGGAC	2 cut(s)	12, 413
BslI	CCNNNNNNNGG	5 cut(s)	35, 36, 99, 338, 436
BsmAI	GTCTC	6 cut(s)	453, 859, 1170, 1194, 1285, 1353
BsmBI	CGTCTC	2 cut(s)	453, 1353
BsmFI	GGGAC	2 cut(s)	12, 413
BsnI	GGCC	3 cut(s)	236, 331, 924
Bso31I	GGTCTC	2 cut(s)	1170, 1194
Bsp1286I	GDGCHC	1 cut(s)	1327
Bsp13I	TCCGGA	2 cut(s)	14, 140
Bsp143I	GATC	3 cut(s)	385, 772, 1364
Bsp19I	CCATGG	1 cut(s)	858
BspANI	GGCC	3 cut(s)	236, 331, 924
BspCNI	CTCAG	2 cut(s)	40, 229
BspEI	TCCGGA	2 cut(s)	14, 140
BspFNI	CGCG	3 cut(s)	171, 309, 482
BspLI	GGNNCC	4 cut(s)	43, 289, 330, 1043
BspMI	ACCTGC	1 cut(s)	1345
BspPI	GGATC	1 cut(s)	1372
BspQI	GCTCTTC	1 cut(s)	166
BspT107I	GGYRCC	1 cut(s)	287
BspTNI	GGTCTC	2 cut(s)	1170, 1194
BsrBI	CCGCTC	1 cut(s)	118
BsrDI	GCAATG	1 cut(s)	798
BsrFI	RCCGGY	2 cut(s)	290, 331
BsrI	ACTGG	5 cut(s)	33, 109, 475, 758, 1384
BssAI	RCCGGY	2 cut(s)	290, 331
BssECI	CCNNGG	4 cut(s)	261, 307, 432, 858
BssMI	GATC	3 cut(s)	385, 772, 1364
BssNAI	GTATAC	1 cut(s)	969
BssNI	GRCGYC	1 cut(s)	288
BssT1I	CCWWGG	2 cut(s)	432, 858
Bst1107I	GTATAC	1 cut(s)	969
Bst2UI	CCWGG	1 cut(s)	1220
Bst4CI	ACNGT	3 cut(s)	400, 958, 986
Bst6I	CTCTTC	2 cut(s)	166, 1052
BstACI	GRCGYC	1 cut(s)	288
BstC8I	GCNNGC	5 cut(s)	238, 292, 296, 828, 1233
BstDEI	CTNAG	2 cut(s)	48, 216
BstDSI	CCRYGG	2 cut(s)	307, 858
BstF5I	GGATG	1 cut(s)	388
BstFNI	CGCG	3 cut(s)	171, 309, 482
BstH2I	RGCGCY	1 cut(s)	291
BstHHI	GCGC	3 cut(s)	290, 482, 1251
BstKTI	GATC	3 cut(s)	388, 775, 1367
BstMAI	GTCTC	6 cut(s)	453, 859, 1170, 1194, 1285, 1353
BstMBI	GATC	3 cut(s)	385, 772, 1364
BstMWI	GCNNNNNNNGC	3 cut(s)	165, 878, 1317
BstNI	CCWGG	1 cut(s)	1220
BstNSI	RCATGY	2 cut(s)	830, 1235
BstSCI	CCNGG	4 cut(s)	245, 260, 361, 1218
BstSFI	CTRYAG	1 cut(s)	198
BstSLI	GKGCMC	1 cut(s)	1327
BstUI	CGCG	3 cut(s)	171, 309, 482
BstV1I	GCAGC	4 cut(s)	69, 884, 963, 1205
BstXI	CCANNNNNNTGG	1 cut(s)	822
BstZ17I	GTATAC	1 cut(s)	969
BsuI	GTATCC	1 cut(s)	460
BsuRI	GGCC	3 cut(s)	236, 331, 924
BtgI	CCRYGG	2 cut(s)	307, 858
BtgZI	GCGATG	1 cut(s)	890
BtsCI	GGATG	1 cut(s)	388
BtsI	GCAGTG	1 cut(s)	971
BtsIMutI	CAGTG	3 cut(s)	40, 45, 971
BveI	ACCTGC	1 cut(s)	1345
Cac8I	GCNNGC	5 cut(s)	238, 292, 296, 828, 1233
CfoI	GCGC	3 cut(s)	290, 482, 1251
Cfr10I	RCCGGY	2 cut(s)	290, 331
Cfr13I	GGNCC	3 cut(s)	304, 329, 1108
Cfr42I	CCGCGG	1 cut(s)	310
CseI	GACGC	1 cut(s)	188
Csp6I	GTAC	3 cut(s)	396, 982, 1012
CspCI	CAANNNNNGTGG	2 cut(s)	34, 69
CviAII	CATG	6 cut(s)	640, 737, 827, 859, 1169, 1232
CviQI	GTAC	3 cut(s)	396, 982, 1012
DdeI	CTNAG	2 cut(s)	48, 216
DinI	GGCGCC	1 cut(s)	289
DpnI	GATC	3 cut(s)	387, 774, 1366
DpnII	GATC	3 cut(s)	385, 772, 1364
DrdI	GACNNNNNNGTC	1 cut(s)	425
DseDI	GACNNNNNNGTC	1 cut(s)	425
Eam1104I	CTCTTC	2 cut(s)	166, 1052
EarI	CTCTTC	2 cut(s)	166, 1052
Eco130I	CCWWGG	2 cut(s)	432, 858
Eco31I	GGTCTC	2 cut(s)	1170, 1194
Eco32I	GATATC	1 cut(s)	1153
Eco47I	GGWCC	2 cut(s)	304, 1108
EcoRII	CCWGG	1 cut(s)	1218
EcoRV	GATATC	1 cut(s)	1153
EcoT14I	CCWWGG	2 cut(s)	432, 858
EcoT22I	ATGCAT	1 cut(s)	1233
EgeI	GGCGCC	1 cut(s)	289
EheI	GGCGCC	1 cut(s)	289
ErhI	CCWWGG	2 cut(s)	432, 858
Esp3I	CGTCTC	2 cut(s)	453, 1353
FaeI	CATG	6 cut(s)	643, 740, 830, 862, 1172, 1235
FaqI	GGGAC	2 cut(s)	12, 413
FatI	CATG	6 cut(s)	639, 736, 826, 858, 1168, 1231
FauI	CCCGC	5 cut(s)	37, 100, 173, 302, 385
FauNDI	CATATG	1 cut(s)	1354
FblI	GTMKAC	1 cut(s)	968
Fnu4HI	GCNGC	6 cut(s)	58, 116, 169, 873, 977, 1194
FokI	GGATG	1 cut(s)	395
Fsp4HI	GCNGC	6 cut(s)	58, 116, 169, 873, 977, 1194
FspBI	CTAG	3 cut(s)	495, 537, 1328
GlaI	GCGC	3 cut(s)	289, 481, 1250
GluI	GCNGC	6 cut(s)	58, 116, 169, 873, 977, 1194
GsaI	CCCAGC	2 cut(s)	159, 465
HaeII	RGCGCY	1 cut(s)	291
HaeIII	GGCC	3 cut(s)	236, 331, 924
HapII	CCGG	9 cut(s)	15, 100, 141, 185, 246, 261, 291, 332, 362
HgaI	GACGC	1 cut(s)	188
HhaI	GCGC	3 cut(s)	290, 482, 1251
Hin1I	GRCGYC	1 cut(s)	288
Hin1II	CATG	6 cut(s)	643, 740, 830, 862, 1172, 1235
Hin6I	GCGC	3 cut(s)	288, 480, 1249
HinP1I	GCGC	3 cut(s)	288, 480, 1249
HincII	GTYRAC	2 cut(s)	193, 954
HindII	GTYRAC	2 cut(s)	193, 954
HinfI	GANTC	7 cut(s)	173, 212, 274, 426, 500, 553, 1017
HpaI	GTTAAC	1 cut(s)	954
HpaII	CCGG	9 cut(s)	15, 100, 141, 185, 246, 261, 291, 332, 362
HphI	GGTGA	1 cut(s)	874
Hpy166II	GTNNAC	5 cut(s)	193, 751, 954, 969, 1256
Hpy188I	TCNGA	5 cut(s)	178, 279, 418, 1285, 1394
Hpy188III	TCNNGA	3 cut(s)	15, 141, 1024
Hpy8I	GTNNAC	5 cut(s)	193, 751, 954, 969, 1256
Hpy99I	CGWCG	6 cut(s)	70, 73, 182, 328, 448, 939
HpyAV	CCTTC	2 cut(s)	1099, 1141
HpyCH4III	ACNGT	3 cut(s)	400, 958, 986
HpyCH4IV	ACGT	1 cut(s)	65
HpyCH4V	TGCA	7 cut(s)	85, 240, 604, 1172, 1193, 1231, 1320
HpyF10VI	GCNNNNNNNGC	3 cut(s)	165, 878, 1317
HpyF3I	CTNAG	2 cut(s)	48, 216
HpySE526I	ACGT	1 cut(s)	65
Hsp92I	GRCGYC	1 cut(s)	288
Hsp92II	CATG	6 cut(s)	643, 740, 830, 862, 1172, 1235
HspAI	GCGC	3 cut(s)	288, 480, 1249
KasI	GGCGCC	1 cut(s)	287
Kpn2I	TCCGGA	2 cut(s)	14, 140
KroI	GCCGGC	1 cut(s)	290
KroNI	GCCGGC	1 cut(s)	292
KspAI	GTTAAC	1 cut(s)	954
KspI	CCGCGG	1 cut(s)	310
Kzo9I	GATC	3 cut(s)	385, 772, 1364
LguI	GCTCTTC	1 cut(s)	166
LmnI	GCTCC	4 cut(s)	41, 187, 1041, 1240
Lsp1109I	GCAGC	4 cut(s)	69, 884, 963, 1205
LweI	GCATC	2 cut(s)	17, 1218
MaeI	CTAG	3 cut(s)	495, 537, 1328
MaeII	ACGT	1 cut(s)	65
MaeIII	GTNAC	3 cut(s)	616, 993, 1140
MalI	GATC	3 cut(s)	387, 774, 1366
MbiI	CCGCTC	1 cut(s)	118
MboI	GATC	3 cut(s)	385, 772, 1364
MboII	GAAGA	8 cut(s)	3, 97, 153, 548, 767, 1012, 1039, 1237
MhlI	GDGCHC	1 cut(s)	1327
MluCI	AATT	2 cut(s)	1239, 1377
Mly113I	GGCGCC	1 cut(s)	288
MlyI	GAGTC	1 cut(s)	435
MmeI	TCCRAC	4 cut(s)	111, 201, 360, 396
Mph1103I	ATGCAT	1 cut(s)	1233
MreI	CGCCGGCG	1 cut(s)	290
MroI	TCCGGA	2 cut(s)	14, 140
MroNI	GCCGGC	1 cut(s)	290
MseI	TTAA	4 cut(s)	663, 953, 1076, 1376
MspA1I	CMGCKG	2 cut(s)	309, 635
MspI	CCGG	9 cut(s)	15, 100, 141, 185, 246, 261, 291, 332, 362
MspR9I	CCNGG	4 cut(s)	247, 262, 363, 1220
MvaI	CCWGG	1 cut(s)	1220
MvnI	CGCG	3 cut(s)	171, 309, 482
MwoI	GCNNNNNNNGC	3 cut(s)	165, 878, 1317
NaeI	GCCGGC	1 cut(s)	292
NarI	GGCGCC	1 cut(s)	288
NciI	CCSGG	3 cut(s)	247, 262, 363
NcoI	CCATGG	1 cut(s)	858
NdeI	CATATG	1 cut(s)	1354
NdeII	GATC	3 cut(s)	385, 772, 1364
NgoMIV	GCCGGC	1 cut(s)	290
NlaIII	CATG	6 cut(s)	643, 740, 830, 862, 1172, 1235
NlaIV	GGNNCC	4 cut(s)	43, 289, 330, 1043
NmuCI	GTSAC	2 cut(s)	616, 1140
NsiI	ATGCAT	1 cut(s)	1233
NspI	RCATGY	2 cut(s)	830, 1235
PaeI	GCATGC	2 cut(s)	830, 1235
PciSI	GCTCTTC	1 cut(s)	166
PcsI	WCGNNNNNNNCGW	2 cut(s)	201, 291
PdiI	GCCGGC	1 cut(s)	292
PfeI	GAWTC	6 cut(s)	173, 212, 274, 500, 553, 1017
PkrI	GCNGC	6 cut(s)	59, 117, 170, 874, 978, 1195
PleI	GAGTC	1 cut(s)	434
PluTI	GGCGCC	1 cut(s)	291
PpsI	GAGTC	1 cut(s)	434
Psp6I	CCWGG	1 cut(s)	1218
PspFI	CCCAGC	2 cut(s)	155, 461
PspGI	CCWGG	1 cut(s)	1218
PspN4I	GGNNCC	4 cut(s)	43, 289, 330, 1043
PspPI	GGNCC	3 cut(s)	304, 329, 1108
PvuII	CAGCTG	1 cut(s)	635
RsaI	GTAC	3 cut(s)	397, 983, 1013
RsaNI	GTAC	3 cut(s)	396, 982, 1012
SacII	CCGCGG	1 cut(s)	310
SapI	GCTCTTC	1 cut(s)	166
SaqAI	TTAA	4 cut(s)	663, 953, 1076, 1376
SatI	GCNGC	6 cut(s)	58, 116, 169, 873, 977, 1194
Sau3AI	GATC	3 cut(s)	385, 772, 1364
Sau96I	GGNCC	3 cut(s)	304, 329, 1108
SchI	GAGTC	1 cut(s)	435
ScrFI	CCNGG	4 cut(s)	247, 262, 363, 1220
SduI	GDGCHC	1 cut(s)	1327
SfaNI	GCATC	2 cut(s)	17, 1218
SfcI	CTRYAG	1 cut(s)	198
SfoI	GGCGCC	1 cut(s)	289
Sfr303I	CCGCGG	1 cut(s)	310
SgrAI	CRCCGGYG	1 cut(s)	290
SgrBI	CCGCGG	1 cut(s)	310
SinI	GGWCC	2 cut(s)	304, 1108
SpeI	ACTAGT	1 cut(s)	1327
SphI	GCATGC	2 cut(s)	830, 1235
Sse9I	AATT	2 cut(s)	1239, 1377
SspDI	GGCGCC	1 cut(s)	287
SspMI	CTAG	3 cut(s)	495, 537, 1328
StyD4I	CCNGG	4 cut(s)	245, 260, 361, 1218
StyI	CCWWGG	2 cut(s)	432, 858
TaaI	ACNGT	3 cut(s)	400, 958, 986
TaiI	ACGT	1 cut(s)	68
TaqI	TCGA	3 cut(s)	251, 272, 763
TasI	AATT	2 cut(s)	1239, 1377
TauI	GCSGC	2 cut(s)	118, 171
TfiI	GAWTC	6 cut(s)	173, 212, 274, 500, 553, 1017
Tru1I	TTAA	4 cut(s)	663, 953, 1076, 1376
Tru9I	TTAA	4 cut(s)	663, 953, 1076, 1376
TscAI	CASTG	3 cut(s)	40, 52, 978
TseFI	GTSAC	2 cut(s)	616, 1140
TseI	GCWGC	4 cut(s)	57, 872, 976, 1193
Tsp45I	GTSAC	2 cut(s)	616, 1140
TspDTI	ATGAA	2 cut(s)	577, 725
TspGWI	ACGGA	3 cut(s)	549, 1159, 1377
TspRI	CASTG	3 cut(s)	40, 52, 978
VpaK11BI	GGWCC	2 cut(s)	304, 1108
XceI	RCATGY	2 cut(s)	830, 1235
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1005
XmiI	GTMKAC	1 cut(s)	968
XspI	CTAG	3 cut(s)	495, 537, 1328
Zsp2I	ATGCAT	1 cut(s)	1233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.