RchiOBHm_Chr1g0372711

RING finger and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003923
Physical Location & Seq
Forward (+)
61279466 .. 61284846
5381 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59668

Sequence Viewer

Length: 1398 bp
ATGGAGGCATCGGCCGGCAATGGGAGCAGTGCGAGCAGTGGAGGGAACAGTAGTGGTGGTGGTGGTGGTGAGGATCGTGCGACGTCGTTTGGCTCTATGCAATTCCCGGCGCTGCGATTTTTCCAGTCGCCGCTTTCTTTTCTGTTAGATTACTCCGGCATACTTTCGCCTACCTCGTCGTCCTCGCGCCACGAGAGCGACGCTCCGGTGGTCAACGCGGGCGTCGTCGCCGATTCGCGGCCGCATTCCCCTGCGGCCTGCACCAGCGGCTCTAGCAACGCGGGGGAGGTTTCGATTCGGATTATCGGCGCCGGGGAGCAGGAGGACCGCGGGAATAGTAACGAAACGACGGCGTTGCAAGGGCAGCGTGGAGAGGAGAATGGAGGGGTGGAAGGGGTGGATAATCAGGCCGGGATGGATGAGAGAGTCCCCTTGGTGGCTCCGTCGTCGCCGGAACATGAGGCCGGGGAGGCTGGGAATGGCGTGGAAGAGAATTCTAGGGATTCGGCTTACCAGAGATATGATATTCAACAGGTAGCCAGGTGGATTGAGCAGGTTCTTCCGTTTTCGCTGCTCTTACTCGTTGTGTTCATCCGTCAGCATTTGCAAGGTTTCTTTGTCACTATATGGATATCAGCTGTCATGTATAGGTCAAATGACATTGTTAGGAAGCAGACGGCTCTAAAGGGAGACAGGAAAATCTCTTTTCTTGTGGGCATTGCGGTTGTTTTTATGCTTCATGTGGTTGGTGTTTACTGGTGGTATCGAAATGACGATCTTTTATACCCGCTGGCAATGCTTCCTCCAAAAGCCATACCACCATTCTGGCATGCAATATTCATCATTTTGGTAAATGATACATTGGTGAGACAGGCAACGATGGCTTTCAAGTGTTTGTTGCTTGTATACTACAAGAATGGAAGAGGGCATAACTATCGTCGACAGGGTCAAATGTTAACCGTAGTTGAGTATTCGCTGCTGCTGTACCGTGCCTTATTACCAGCACCTGTTTGGTACAGATTCTTCCTGAACAAAGAATATGGAAGCCTCTTCTCATCACTTATTACAGGATTATACTTGACTTTTAAGCTAACATCTGTTGTTGAGAAGGTCCAATCCTTTTTTGCTTCTATAAGGGCATTATCACGAAAAGATATCCATTATGGGTCCCATGCAACATTGGAACAGGTAAATGCAGCAGGAGACCTTTGTGCCATTTGCCAGGAGAAGATGCATGCTCCTATCCTGTTGCGCTGCAAACACATCTTCTGCGAGGACTGTGTTTCCGAATGGTTTGAGAGAGAAAGAACTTGCCCTCTATGCCGGGCAGTGGTAAAACCTGCAGATCTGCGAACGTTTGGGGATGGATCAACAAGTTTATTTTTCCAGTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

465

Amino Acids

51.42

Weight (kDa)

6.43

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 401 - 442 5.9e-06 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 402 - 445 4.7e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 403 - 442 1.9e-10 Ring finger domain
zf-C3HC4 PF00097 404 - 441 3.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 404 - 441 6.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 404 - 439 7.8e-08 RING-type zinc-finger
zf-RING_5 PF14634 404 - 442 2.7e-07 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 86
Acc36I ACCTGC 2 cut(s) 544, 1348
AccB1I GGYRCC 1 cut(s) 308
AccI GTMKAC 2 cut(s) 906, 940
AccII CGCG 5 cut(s) 187, 218, 238, 281, 330
AclI AACGTT 1 cut(s) 1355
AclWI GGATC 2 cut(s) 81, 1375
AcoI YGGCCR 2 cut(s) 12, 239
AcsI RAATTY 1 cut(s) 493
AcyI GRCGYC 3 cut(s) 83, 222, 309
AfaI GTAC 2 cut(s) 986, 1016
AfiI CCNNNNNNNGG 4 cut(s) 21, 237, 436, 1330
AgsI TTSAA 2 cut(s) 530, 889
AjnI CCWGG 2 cut(s) 539, 1221
AluBI AGCT 2 cut(s) 638, 1090
AluI AGCT 2 cut(s) 638, 1090
Alw26I GTCTC 3 cut(s) 684, 862, 1197
AlwI GGATC 2 cut(s) 81, 1375
AlwNI CAGNNNCTG 1 cut(s) 1007
AoxI GGCC 5 cut(s) 12, 239, 255, 408, 462
ApeKI GCWGC 7 cut(s) 112, 364, 571, 976, 979, 1196, 1254
ApoI RAATTY 1 cut(s) 493
AspLEI GCGC 4 cut(s) 112, 189, 311, 1254
AspS9I GGNCC 3 cut(s) 325, 1111, 1167
AsuC2I CCSGG 5 cut(s) 107, 313, 412, 466, 1325
AsuHPI GGTGA 2 cut(s) 80, 877
AvaII GGWCC 3 cut(s) 325, 1111, 1167
BaeI ACNNNNGTAYC 2 cut(s) 849, 882
BanI GGYRCC 1 cut(s) 308
BarI GAAGNNNNNNTAC 2 cut(s) 1007, 1039
BauI CACGAG 1 cut(s) 191
BbvI GCAGC 7 cut(s) 99, 376, 558, 963, 966, 1208, 1241
BccI CCATC 3 cut(s) 409, 874, 1358
BceAI ACGGC 2 cut(s) 366, 693
BcgI CGANNNNNNTGC 4 cut(s) 337, 371, 917, 951
BciT130I CCWGG 2 cut(s) 541, 1223
BcnI CCSGG 5 cut(s) 107, 313, 412, 466, 1325
BcoDI GTCTC 3 cut(s) 684, 862, 1197
BfaI CTAG 2 cut(s) 273, 498
BfmI CTRYAG 1 cut(s) 1341
BfoI RGCGCY 2 cut(s) 113, 312
BfuAI ACCTGC 2 cut(s) 544, 1348
BglI GCCNNNNNGGC 1 cut(s) 470
BglII AGATCT 1 cut(s) 1345
Bme1390I CCNGG 7 cut(s) 107, 313, 412, 466, 541, 1223, 1325
Bme18I GGWCC 3 cut(s) 325, 1111, 1167
BmgT120I GGNCC 3 cut(s) 325, 1111, 1167
BmiI GGNNCC 4 cut(s) 310, 441, 1168, 1169
BmrFI CCNGG 7 cut(s) 107, 313, 412, 466, 541, 1223, 1325
BmsI GCATC 2 cut(s) 17, 1221
BplI GAGNNNNNCTC 2 cut(s) 187, 219
BpuMI CCSGG 5 cut(s) 107, 313, 412, 466, 1325
BsaHI GRCGYC 3 cut(s) 83, 222, 309
BsaI GGTCTC 1 cut(s) 1197
BsaJI CCNNGG 4 cut(s) 312, 328, 432, 465
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 237, 436, 1330
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 3 cut(s) 124, 761, 1387
Bse3DI GCAATG 3 cut(s) 25, 717, 801
BseBI CCWGG 2 cut(s) 541, 1223
BseDI CCNNGG 4 cut(s) 312, 328, 432, 465
BseGI GGATG 4 cut(s) 420, 424, 591, 1369
BseLI CCNNNNNNNGG 4 cut(s) 21, 237, 436, 1330
BseMI GCAATG 3 cut(s) 25, 717, 801
BseNI ACTGG 3 cut(s) 124, 761, 1387
BseRI GAGGAG 1 cut(s) 389
BseX3I CGGCCG 2 cut(s) 12, 239
BseXI GCAGC 7 cut(s) 99, 376, 558, 963, 966, 1208, 1241
BseYI CCCAGC 1 cut(s) 473
BsgI GTGCAG 1 cut(s) 244
Bsh1236I CGCG 5 cut(s) 187, 218, 238, 281, 330
Bsh1285I CGRYCG 2 cut(s) 15, 242
BshFI GGCC 5 cut(s) 14, 241, 257, 410, 464
BshNI GGYRCC 1 cut(s) 308
BsiEI CGRYCG 2 cut(s) 15, 242
BsiSI CCGG 9 cut(s) 15, 107, 156, 206, 312, 411, 452, 465, 1324
BslFI GGGAC 2 cut(s) 413, 1153
BslI CCNNNNNNNGG 4 cut(s) 21, 237, 436, 1330
BsmAI GTCTC 3 cut(s) 684, 862, 1197
BsmFI GGGAC 2 cut(s) 413, 1153
BsmI GAATGC 1 cut(s) 244
BsnI GGCC 5 cut(s) 14, 241, 257, 410, 464
Bso31I GGTCTC 1 cut(s) 1197
Bsp143I GATC 4 cut(s) 73, 775, 1345, 1367
BspANI GGCC 5 cut(s) 14, 241, 257, 410, 464
BspFNI CGCG 5 cut(s) 187, 218, 238, 281, 330
BspLI GGNNCC 4 cut(s) 310, 441, 1168, 1169
BspMAI CTGCAG 1 cut(s) 1345
BspMI ACCTGC 2 cut(s) 544, 1348
BspPI GGATC 2 cut(s) 81, 1375
BspT107I GGYRCC 1 cut(s) 308
BspTNI GGTCTC 1 cut(s) 1197
BsrDI GCAATG 3 cut(s) 25, 717, 801
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 3 cut(s) 124, 761, 1387
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 4 cut(s) 312, 328, 432, 465
BssMI GATC 4 cut(s) 73, 775, 1345, 1367
BssNAI GTATAC 1 cut(s) 907
BssNI GRCGYC 3 cut(s) 83, 222, 309
BssSI CACGAG 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 432
Bst1107I GTATAC 1 cut(s) 907
Bst2BI CACGAG 1 cut(s) 191
Bst2UI CCWGG 2 cut(s) 541, 1223
Bst4CI ACNGT 4 cut(s) 50, 961, 989, 1280
Bst6I CTCTTC 3 cut(s) 483, 916, 1055
BstACI GRCGYC 3 cut(s) 83, 222, 309
BstC8I GCNNGC 7 cut(s) 16, 34, 220, 259, 792, 831, 1236
BstDSI CCRYGG 1 cut(s) 328
BstF5I GGATG 4 cut(s) 420, 424, 591, 1369
BstFNI CGCG 5 cut(s) 187, 218, 238, 281, 330
BstH2I RGCGCY 2 cut(s) 113, 312
BstHHI GCGC 4 cut(s) 112, 189, 311, 1254
BstKTI GATC 4 cut(s) 76, 778, 1348, 1370
BstMAI GTCTC 3 cut(s) 684, 862, 1197
BstMBI GATC 4 cut(s) 73, 775, 1345, 1367
BstMCI CGRYCG 2 cut(s) 15, 242
BstNI CCWGG 2 cut(s) 541, 1223
BstNSI RCATGY 2 cut(s) 833, 1238
BstSCI CCNGG 7 cut(s) 105, 311, 410, 464, 539, 1221, 1323
BstSFI CTRYAG 1 cut(s) 1341
BstUI CGCG 5 cut(s) 187, 218, 238, 281, 330
BstV1I GCAGC 7 cut(s) 99, 376, 558, 963, 966, 1208, 1241
BstX2I RGATCY 1 cut(s) 1345
BstXI CCANNNNNNTGG 1 cut(s) 825
BstYI RGATCY 1 cut(s) 1345
BstZ17I GTATAC 1 cut(s) 907
BstZI CGGCCG 2 cut(s) 12, 239
BsuRI GGCC 5 cut(s) 14, 241, 257, 410, 464
BtgI CCRYGG 1 cut(s) 328
BtsCI GGATG 4 cut(s) 420, 424, 591, 1369
BtsI GCAGTG 3 cut(s) 34, 43, 1335
BtsIMutI CAGTG 3 cut(s) 34, 43, 1335
BveI ACCTGC 2 cut(s) 544, 1348
Cac8I GCNNGC 7 cut(s) 16, 34, 220, 259, 792, 831, 1236
CaiI CAGNNNCTG 1 cut(s) 1007
CciNI GCGGCCGC 1 cut(s) 239
CfoI GCGC 4 cut(s) 112, 189, 311, 1254
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 3 cut(s) 325, 1111, 1167
Cfr42I CCGCGG 1 cut(s) 331
CseI GACGC 2 cut(s) 209, 211
Csp6I GTAC 2 cut(s) 985, 1015
CviAII CATG 6 cut(s) 458, 643, 740, 830, 1172, 1235
CviQI GTAC 2 cut(s) 985, 1015
DinI GGCGCC 1 cut(s) 310
DpnI GATC 4 cut(s) 75, 777, 1347, 1369
DpnII GATC 4 cut(s) 73, 775, 1345, 1367
EaeI YGGCCR 2 cut(s) 12, 239
EagI CGGCCG 2 cut(s) 12, 239
Eam1104I CTCTTC 3 cut(s) 483, 916, 1055
EarI CTCTTC 3 cut(s) 483, 916, 1055
EclXI CGGCCG 2 cut(s) 12, 239
Eco130I CCWWGG 1 cut(s) 432
Eco31I GGTCTC 1 cut(s) 1197
Eco32I GATATC 2 cut(s) 633, 1156
Eco47I GGWCC 3 cut(s) 325, 1111, 1167
Eco52I CGGCCG 2 cut(s) 12, 239
EcoO109I RGGNCCY 1 cut(s) 1167
EcoRI GAATTC 1 cut(s) 493
EcoRII CCWGG 2 cut(s) 539, 1221
EcoRV GATATC 2 cut(s) 633, 1156
EcoT14I CCWWGG 1 cut(s) 432
EcoT22I ATGCAT 1 cut(s) 1236
EgeI GGCGCC 1 cut(s) 310
EheI GGCGCC 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 6 cut(s) 461, 646, 743, 833, 1175, 1238
FaqI GGGAC 2 cut(s) 413, 1153
FatI CATG 6 cut(s) 457, 642, 739, 829, 1171, 1234
FauI CCCGC 4 cut(s) 211, 274, 323, 795
FblI GTMKAC 2 cut(s) 906, 940
FokI GGATG 4 cut(s) 427, 431, 578, 1376
FspBI CTAG 2 cut(s) 273, 498
GlaI GCGC 4 cut(s) 111, 188, 310, 1253
GsaI CCCAGC 1 cut(s) 477
HaeII RGCGCY 2 cut(s) 113, 312
HaeIII GGCC 5 cut(s) 14, 241, 257, 410, 464
HapII CCGG 9 cut(s) 15, 107, 156, 206, 312, 411, 452, 465, 1324
HgaI GACGC 2 cut(s) 209, 211
HhaI GCGC 4 cut(s) 112, 189, 311, 1254
Hin1I GRCGYC 3 cut(s) 83, 222, 309
Hin1II CATG 6 cut(s) 461, 646, 743, 833, 1175, 1238
Hin6I GCGC 4 cut(s) 110, 187, 309, 1252
HinP1I GCGC 4 cut(s) 110, 187, 309, 1252
HincII GTYRAC 3 cut(s) 214, 941, 957
HindII GTYRAC 3 cut(s) 214, 941, 957
HinfI GANTC 5 cut(s) 233, 295, 426, 503, 1020
HpaI GTTAAC 1 cut(s) 957
HpaII CCGG 9 cut(s) 15, 107, 156, 206, 312, 411, 452, 465, 1324
HphI GGTGA 2 cut(s) 80, 877
Hpy166II GTNNAC 5 cut(s) 214, 754, 907, 941, 957
Hpy188I TCNGA 3 cut(s) 300, 1288, 1397
Hpy188III TCNNGA 2 cut(s) 1027, 1146
Hpy8I GTNNAC 5 cut(s) 214, 754, 907, 941, 957
HpyAV CCTTC 2 cut(s) 386, 1102
HpyCH4III ACNGT 4 cut(s) 50, 961, 989, 1280
HpyCH4IV ACGT 2 cut(s) 83, 1355
HpySE526I ACGT 2 cut(s) 83, 1355
Hsp92I GRCGYC 3 cut(s) 83, 222, 309
Hsp92II CATG 6 cut(s) 461, 646, 743, 833, 1175, 1238
HspAI GCGC 4 cut(s) 110, 187, 309, 1252
KasI GGCGCC 1 cut(s) 308
KflI GGGWCCC 1 cut(s) 1167
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
KspAI GTTAAC 1 cut(s) 957
KspI CCGCGG 1 cut(s) 331
Kzo9I GATC 4 cut(s) 73, 775, 1345, 1367
LmnI GCTCC 5 cut(s) 24, 208, 316, 445, 1243
Lsp1109I GCAGC 7 cut(s) 99, 376, 558, 963, 966, 1208, 1241
LweI GCATC 2 cut(s) 17, 1221
MaeI CTAG 2 cut(s) 273, 498
MaeII ACGT 2 cut(s) 83, 1355
MaeIII GTNAC 2 cut(s) 338, 619
MalI GATC 4 cut(s) 75, 777, 1347, 1369
MboI GATC 4 cut(s) 73, 775, 1345, 1367
MboII GAAGA 8 cut(s) 500, 551, 933, 1015, 1042, 1240, 1258, 1384
MflI RGATCY 1 cut(s) 1345
MluCI AATT 2 cut(s) 101, 493
Mly113I GGCGCC 1 cut(s) 309
MlyI GAGTC 1 cut(s) 435
Mph1103I ATGCAT 1 cut(s) 1236
MroNI GCCGGC 1 cut(s) 14
MseI TTAA 2 cut(s) 956, 1086
MspA1I CMGCKG 4 cut(s) 267, 330, 638, 790
MspI CCGG 9 cut(s) 15, 107, 156, 206, 312, 411, 452, 465, 1324
MspR9I CCNGG 7 cut(s) 107, 313, 412, 466, 541, 1223, 1325
Mva1269I GAATGC 1 cut(s) 244
MvaI CCWGG 2 cut(s) 541, 1223
MvnI CGCG 5 cut(s) 187, 218, 238, 281, 330
NaeI GCCGGC 1 cut(s) 16
NarI GGCGCC 1 cut(s) 309
NciI CCSGG 5 cut(s) 107, 313, 412, 466, 1325
NdeII GATC 4 cut(s) 73, 775, 1345, 1367
NgoMIV GCCGGC 1 cut(s) 14
NlaIII CATG 6 cut(s) 461, 646, 743, 833, 1175, 1238
NlaIV GGNNCC 4 cut(s) 310, 441, 1168, 1169
NmuCI GTSAC 1 cut(s) 619
NotI GCGGCCGC 1 cut(s) 239
NsiI ATGCAT 1 cut(s) 1236
NspI RCATGY 2 cut(s) 833, 1238
PaeI GCATGC 2 cut(s) 833, 1238
PcsI WCGNNNNNNNCGW 2 cut(s) 173, 222
PctI GAATGC 1 cut(s) 244
PdiI GCCGGC 1 cut(s) 16
PfeI GAWTC 4 cut(s) 233, 295, 503, 1020
PflFI GACNNNGTC 1 cut(s) 945
PleI GAGTC 1 cut(s) 434
PluTI GGCGCC 1 cut(s) 312
PpsI GAGTC 1 cut(s) 434
PpuMI RGGWCCY 1 cut(s) 1167
Psp1406I AACGTT 1 cut(s) 1355
Psp5II RGGWCCY 1 cut(s) 1167
Psp6I CCWGG 2 cut(s) 539, 1221
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 2 cut(s) 539, 1221
PspN4I GGNNCC 4 cut(s) 310, 441, 1168, 1169
PspPI GGNCC 3 cut(s) 325, 1111, 1167
PspPPI RGGWCCY 1 cut(s) 1167
PstI CTGCAG 1 cut(s) 1345
PstNI CAGNNNCTG 1 cut(s) 1007
PsuI RGATCY 1 cut(s) 1345
PsyI GACNNNGTC 1 cut(s) 945
PvuII CAGCTG 1 cut(s) 638
RsaI GTAC 2 cut(s) 986, 1016
RsaNI GTAC 2 cut(s) 985, 1015
SacII CCGCGG 1 cut(s) 331
SalI GTCGAC 1 cut(s) 939
SaqAI TTAA 2 cut(s) 956, 1086
Sau3AI GATC 4 cut(s) 73, 775, 1345, 1367
Sau96I GGNCC 3 cut(s) 325, 1111, 1167
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 7 cut(s) 107, 313, 412, 466, 541, 1223, 1325
SfaNI GCATC 2 cut(s) 17, 1221
SfcI CTRYAG 1 cut(s) 1341
SfoI GGCGCC 1 cut(s) 310
Sfr303I CCGCGG 1 cut(s) 331
SgrBI CCGCGG 1 cut(s) 331
SinI GGWCC 3 cut(s) 325, 1111, 1167
SphI GCATGC 2 cut(s) 833, 1238
Sse9I AATT 2 cut(s) 101, 493
SspDI GGCGCC 1 cut(s) 308
SspI AATATT 1 cut(s) 837
SspMI CTAG 2 cut(s) 273, 498
StyD4I CCNGG 7 cut(s) 105, 311, 410, 464, 539, 1221, 1323
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 4 cut(s) 50, 961, 989, 1280
TaiI ACGT 2 cut(s) 86, 1358
TaqI TCGA 3 cut(s) 293, 766, 940
TasI AATT 2 cut(s) 101, 493
TauI GCSGC 5 cut(s) 133, 241, 244, 257, 270
TfiI GAWTC 4 cut(s) 233, 295, 503, 1020
Tru1I TTAA 2 cut(s) 956, 1086
Tru9I TTAA 2 cut(s) 956, 1086
TscAI CASTG 3 cut(s) 34, 43, 1335
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 7 cut(s) 112, 364, 571, 976, 979, 1196, 1254
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 3 cut(s) 580, 728, 829
TspGWI ACGGA 3 cut(s) 432, 552, 584
TspRI CASTG 3 cut(s) 34, 43, 1335
Tth111I GACNNNGTC 1 cut(s) 945
VpaK11BI GGWCC 3 cut(s) 325, 1111, 1167
XapI RAATTY 1 cut(s) 493
XceI RCATGY 2 cut(s) 833, 1238
XcmI CCANNNNNNNNNTGG 1 cut(s) 1008
XmiI GTMKAC 2 cut(s) 906, 940
XspI CTAG 2 cut(s) 273, 498
ZraI GACGTC 1 cut(s) 84
Zsp2I ATGCAT 1 cut(s) 1236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.