RLG00000025058

RING finger and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003923
Physical Location & Seq
Forward (+)
41643392 .. 41648128
4737 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025058

Sequence Viewer

Length: 1839 bp
ATGGCACCCGAATGTAACTCAAAACTTCAAACCAAGCTAAGCTTTGGGGTATTCCCCCACTTTCTCTTCTCGCGCTCTTCCTCATTGAGCGAGAAAGAGAACCCGCCAAACGCAACCCAAATTACCCAAAACCAATTCTTGAATTCCATTCCCAAATTCCCAAACCTCACACATACCCAACCCCCAATCACCACCACCACCATCATCATCATCATCAACCTCTCCCACATTTCTCTGCAAACCCTAGCCTTCCACTCATCATCGCGCAATTCCATCCGGCAATCGCTGCTCTCCGGCCCTCCCAAGATTCCGTCTTGGCAGCGCCGCCGGCGCGATTTCGTCCGGTCCGCGTTTCTCCGATGCCGTCTCTCCCCTGAATCCCCATCACCACTGCGCTTTCGAAGGGTTTGGGCGTTTAGGCAAATGGAAACGCCAGGTGGCAATCATCTGGATTCGTATAGAGCTCCGGTTTCGAGCAATAGTAGTTCTAGAAGATATGGAATGCTGTCTGCTTCCAATTTTATTCAGGCACCACTGTCTGCCTTGCTGGAGTACTCGGGCCTTCTGCGCGGCCGGTCCAGTCACCAAGAAGCCGAGTCTTTGATTGGGGGACGTAGCGCTGCCGCGTTCAGGGAGCACCATCACGGTCAGGTGGATCAATCGAGCAATGATGGGGAGGTTTCCATTAGGATAATTGGTGCAGGGGAGCAAGAGCACGATAGGGAAGGGGGTGGTTTAGTAGTTGGACAGTTGAGGGAGAGTAGTCTTCTGCAAAATGATGTTTCCGGACAACCAATAGCTAGGGTGGGCTCTGTTACAGTGGTATCGGAGGAGGGTCAGGGAGATGGTAGAACCGATGTTGGTTCTGGAGAAGGCATGTCACAATCGATAAATGGAAGTGCTGATGGAGAAGCTGGTGATGGTGCTGGGGCTAATGGCAGGGATTCTTCTTACCAGAGATATGATATTCAGCAGGCAGCTAGGTGGATTGAGCAGGTCCTGCCCTTTTCCTTGCTTCTATTGGTGGTCTTCATCCGCCAGCATTTGCAAGGTTTCTTTGTTACCATCTGGATTGCTGCTGTTATGTTTAAGTCGAATGATATCCTAAGGAAACAGACAGCTTTGAAGGGAGAGAGGAAAATCTCTGTTCTAGTCGGCATCTCTATTGCATTTGCAGTTCATGTGATTGGTGTATACTGGTGGTATCAGAATGATGATCTTTTGTACCCATTAGTTATGCTTCCTCCCAAAGTTATACCACCTTTCTGGCATGCTGTTTTTATCATCATGGTTAATGATACCTTGGTCCGGCAGGCAGCAATGGTGATCAAGTGTATTCTATTAATGTACTACAAGAACAGCAGAGGACGTAACTATCGTAAGCAGGGTCAAATGCTTACATTGGTTGAGTATCTACTTCTGTTGTACCGGGCCTTGTTACCAACACCAGTGTGGTATCGTTTCTTCTTGAACAAAGAATATGGTAGTCTCTTTTCTTCACTAATGACAGGGTTATATTTGACCTTCAAGCTTACGTCTGTTGTTGAGAAGGTACAATCTTTCTTTGCTGCATTGAAGGCCTTGTCACGTAAAGAGGTTCATTATGGGGCGTATGCAACATCAGAGCAGGTCAGTGCAGCTGGTGATCTATGTGCTATCTGCCAGGAGAAAATGCATGCTCCTATCTTGCTTCGTTGTAAACACATCTTCTGTGAGGACTGTGTATCAGAGTGGTTTGAGAGGGAACGGACCTGCCCGTTGTGCAGGGCTTTGGTAAAACCTGCAGATCTTAGATCGTTTGGCGATGGATCAACAAGCTTGTTCTTCCAGCTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3 zf-RING_2

Protein Analysis

613

Amino Acids

68.54

Weight (kDa)

9.34

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 549 - 592 8.8e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 550 - 589 2.7e-10 Ring finger domain
zf-C3HC4 PF00097 551 - 588 4.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 551 - 588 8.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 551 - 586 1.1e-07 RING-type zinc-finger
zf-RING_5 PF14634 551 - 589 3.8e-07 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 985, 1618, 1760, 1789
AccB1I GGYRCC 2 cut(s) 4, 529
AccI GTMKAC 1 cut(s) 1194
AccII CGCG 6 cut(s) 73, 265, 333, 350, 570, 626
AccIII TCCGGA 1 cut(s) 785
AciI CCGC 6 cut(s) 104, 325, 348, 570, 624, 1036
AclWI GGATC 2 cut(s) 663, 1816
AcoI YGGCCR 1 cut(s) 571
AcsI RAATTY 2 cut(s) 142, 155
AfaI GTAC 5 cut(s) 554, 1226, 1349, 1427, 1554
AfeI AGCGCT 1 cut(s) 619
AfiI CCNNNNNNNGG 2 cut(s) 630, 1308
AgsI TTSAA 6 cut(s) 29, 142, 1126, 1471, 1528, 1576
AjnI CCWGG 2 cut(s) 433, 1662
AleI CACNNNNGTG 1 cut(s) 1450
Alw21I GWGCWC 3 cut(s) 466, 639, 717
Alw26I GTCTC 2 cut(s) 371, 1493
AlwI GGATC 2 cut(s) 663, 1816
AlwNI CAGNNNCTG 1 cut(s) 1000
Ama87I CYCGRG 1 cut(s) 556
Aor13HI TCCGGA 1 cut(s) 785
Aor51HI AGCGCT 1 cut(s) 619
AoxI GGCC 5 cut(s) 295, 559, 571, 1431, 1578
ApeKI GCWGC 8 cut(s) 286, 319, 620, 977, 1076, 1316, 1568, 1637
ApoI RAATTY 2 cut(s) 142, 155
AseI ATTAAT 1 cut(s) 1343
AspLEI GCGC 7 cut(s) 75, 267, 324, 333, 396, 570, 620
AspS9I GGNCC 8 cut(s) 296, 345, 559, 576, 997, 1306, 1431, 1749
AsuC2I CCSGG 1 cut(s) 1430
AsuHPI GGTGA 6 cut(s) 181, 378, 575, 929, 1336, 1655
AsuII TTCGAA 1 cut(s) 400
AvaI CYCGRG 1 cut(s) 556
AvaII GGWCC 5 cut(s) 345, 576, 997, 1306, 1749
AxyI CCTNAGG 1 cut(s) 1106
BaeI ACNNNNGTAYC 4 cut(s) 807, 840, 1290, 1323
BanI GGYRCC 2 cut(s) 4, 529
BanII GRGCYC 2 cut(s) 466, 812
BarI GAAGNNNNNNTAC 2 cut(s) 1448, 1480
BbsI GAAGAC 2 cut(s) 758, 1021
Bbv12I GWGCWC 3 cut(s) 466, 639, 717
BbvI GCAGC 8 cut(s) 273, 331, 607, 989, 1063, 1328, 1555, 1649
BceAI ACGGC 1 cut(s) 348
BciT130I CCWGG 2 cut(s) 435, 1664
BclI TGATCA 1 cut(s) 1326
BcnI CCSGG 1 cut(s) 1430
BcoDI GTCTC 2 cut(s) 371, 1493
BfaI CTAG 5 cut(s) 245, 489, 801, 981, 1151
BfmI CTRYAG 1 cut(s) 1782
BfoI RGCGCY 2 cut(s) 325, 621
BfuAI ACCTGC 4 cut(s) 985, 1618, 1760, 1789
BglII AGATCT 1 cut(s) 1786
BlpI GCTNAGC 1 cut(s) 38
BmcAI AGTACT 1 cut(s) 554
Bme1390I CCNGG 3 cut(s) 435, 1430, 1664
Bme18I GGWCC 5 cut(s) 345, 576, 997, 1306, 1749
BmeT110I CYCGRG 1 cut(s) 556
BmgT120I GGNCC 8 cut(s) 296, 345, 559, 576, 997, 1306, 1431, 1749
BmiI GGNNCC 2 cut(s) 6, 531
BmrFI CCNGG 3 cut(s) 435, 1430, 1664
BmsI GCATC 2 cut(s) 350, 1167
BpiI GAAGAC 2 cut(s) 758, 1021
BpmI CTGGAG 2 cut(s) 569, 888
Bpu1102I GCTNAGC 1 cut(s) 38
Bpu14I TTCGAA 1 cut(s) 400
BpuMI CCSGG 1 cut(s) 1430
Bsa29I ATCGAT 1 cut(s) 887
BsaAI YACGTR 1 cut(s) 1589
BsaJI CCNNGG 1 cut(s) 1302
BsaWI WCCGGW 3 cut(s) 342, 466, 785
Bsc4I CCNNNNNNNGG 2 cut(s) 630, 1308
Bse118I RCCGGY 2 cut(s) 327, 573
Bse1I ACTGG 3 cut(s) 579, 1202, 1448
Bse21I CCTNAGG 1 cut(s) 1106
Bse3DI GCAATG 2 cut(s) 673, 1326
BseAI TCCGGA 1 cut(s) 785
BseBI CCWGG 2 cut(s) 435, 1664
BseCI ATCGAT 1 cut(s) 887
BseDI CCNNGG 1 cut(s) 1302
BseGI GGATG 2 cut(s) 273, 1032
BseLI CCNNNNNNNGG 2 cut(s) 630, 1308
BseMI GCAATG 2 cut(s) 673, 1326
BseNI ACTGG 3 cut(s) 579, 1202, 1448
BseRI GAGGAG 1 cut(s) 845
BseX3I CGGCCG 1 cut(s) 571
BseXI GCAGC 8 cut(s) 273, 331, 607, 989, 1063, 1328, 1555, 1649
BseYI CCCAGC 1 cut(s) 926
BsgI GTGCAG 3 cut(s) 720, 1656, 1783
Bsh1236I CGCG 6 cut(s) 73, 265, 333, 350, 570, 626
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 5 cut(s) 297, 561, 573, 1433, 1580
BshNI GGYRCC 2 cut(s) 4, 529
BshVI ATCGAT 1 cut(s) 887
BsiEI CGRYCG 1 cut(s) 574
BsiHKAI GWGCWC 3 cut(s) 466, 639, 717
BsiHKCI CYCGRG 1 cut(s) 556
BsiSI CCGG 9 cut(s) 277, 294, 328, 343, 467, 574, 786, 1309, 1429
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 2 cut(s) 630, 1308
BsmAI GTCTC 2 cut(s) 371, 1493
BsmBI CGTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 624
BsmI GAATGC 1 cut(s) 507
BsnI GGCC 5 cut(s) 297, 561, 573, 1433, 1580
BsoBI CYCGRG 1 cut(s) 556
Bsp119I TTCGAA 1 cut(s) 400
Bsp1286I GDGCHC 4 cut(s) 466, 639, 717, 812
Bsp13I TCCGGA 1 cut(s) 785
Bsp143I GATC 7 cut(s) 655, 1216, 1326, 1645, 1786, 1793, 1808
Bsp1720I GCTNAGC 1 cut(s) 38
BspACI CCGC 6 cut(s) 104, 325, 348, 570, 624, 1036
BspANI GGCC 5 cut(s) 297, 561, 573, 1433, 1580
BspDI ATCGAT 1 cut(s) 887
BspEI TCCGGA 1 cut(s) 785
BspFNI CGCG 6 cut(s) 73, 265, 333, 350, 570, 626
BspLI GGNNCC 2 cut(s) 6, 531
BspMAI CTGCAG 1 cut(s) 1786
BspMI ACCTGC 4 cut(s) 985, 1618, 1760, 1789
BspPI GGATC 2 cut(s) 663, 1816
BspQI GCTCTTC 1 cut(s) 82
BspT104I TTCGAA 1 cut(s) 400
BspT107I GGYRCC 2 cut(s) 4, 529
BsrDI GCAATG 2 cut(s) 673, 1326
BsrFI RCCGGY 2 cut(s) 327, 573
BsrI ACTGG 3 cut(s) 579, 1202, 1448
BssAI RCCGGY 2 cut(s) 327, 573
BssECI CCNNGG 1 cut(s) 1302
BssMI GATC 7 cut(s) 655, 1216, 1326, 1645, 1786, 1793, 1808
BssNAI GTATAC 1 cut(s) 1195
BssT1I CCWWGG 1 cut(s) 1302
Bst1107I GTATAC 1 cut(s) 1195
Bst2UI CCWGG 2 cut(s) 435, 1664
Bst4CI ACNGT 5 cut(s) 537, 647, 750, 820, 1721
Bst6I CTCTTC 2 cut(s) 71, 82
BstAPI GCANNNNNTGC 2 cut(s) 286, 1000
BstBAI YACGTR 1 cut(s) 1589
BstBI TTCGAA 1 cut(s) 400
BstC8I GCNNGC 6 cut(s) 329, 975, 1040, 1272, 1314, 1677
BstDEI CTNAG 3 cut(s) 38, 1106, 1790
BstF5I GGATG 2 cut(s) 273, 1032
BstFNI CGCG 6 cut(s) 73, 265, 333, 350, 570, 626
BstH2I RGCGCY 2 cut(s) 325, 621
BstHHI GCGC 7 cut(s) 75, 267, 324, 333, 396, 570, 620
BstKTI GATC 7 cut(s) 658, 1219, 1329, 1648, 1789, 1796, 1811
BstMAI GTCTC 2 cut(s) 371, 1493
BstMBI GATC 7 cut(s) 655, 1216, 1326, 1645, 1786, 1793, 1808
BstMCI CGRYCG 1 cut(s) 574
BstMWI GCNNNNNNNGC 7 cut(s) 286, 328, 330, 567, 1000, 1577, 1761
BstNI CCWGG 2 cut(s) 435, 1664
BstNSI RCATGY 3 cut(s) 880, 1274, 1679
BstSCI CCNGG 3 cut(s) 433, 1428, 1662
BstSFI CTRYAG 1 cut(s) 1782
BstUI CGCG 6 cut(s) 73, 265, 333, 350, 570, 626
BstV1I GCAGC 8 cut(s) 273, 331, 607, 989, 1063, 1328, 1555, 1649
BstV2I GAAGAC 2 cut(s) 758, 1021
BstX2I RGATCY 1 cut(s) 1786
BstXI CCANNNNNNTGG 1 cut(s) 1266
BstYI RGATCY 1 cut(s) 1786
BstZ17I GTATAC 1 cut(s) 1195
BstZI CGGCCG 1 cut(s) 571
Bsu15I ATCGAT 1 cut(s) 887
Bsu36I CCTNAGG 1 cut(s) 1106
BsuRI GGCC 5 cut(s) 297, 561, 573, 1433, 1580
BsuTUI ATCGAT 1 cut(s) 887
BtgZI GCGATG 2 cut(s) 246, 1818
BtsCI GGATG 2 cut(s) 273, 1032
BtsI GCAGTG 1 cut(s) 389
BtsIMutI CAGTG 5 cut(s) 389, 533, 825, 1455, 1639
BveI ACCTGC 4 cut(s) 985, 1618, 1760, 1789
Cac8I GCNNGC 6 cut(s) 329, 975, 1040, 1272, 1314, 1677
CaiI CAGNNNCTG 1 cut(s) 1000
CfoI GCGC 7 cut(s) 75, 267, 324, 333, 396, 570, 620
Cfr10I RCCGGY 2 cut(s) 327, 573
Cfr13I GGNCC 8 cut(s) 296, 345, 559, 576, 997, 1306, 1431, 1749
ClaI ATCGAT 1 cut(s) 887
CpoI CGGWCCG 1 cut(s) 345
Csp6I GTAC 5 cut(s) 553, 1225, 1348, 1426, 1553
CspI CGGWCCG 1 cut(s) 345
CviAII CATG 5 cut(s) 877, 1181, 1271, 1288, 1676
CviQI GTAC 5 cut(s) 553, 1225, 1348, 1426, 1553
DdeI CTNAG 3 cut(s) 38, 1106, 1790
DpnI GATC 7 cut(s) 657, 1218, 1328, 1647, 1788, 1795, 1810
DpnII GATC 7 cut(s) 655, 1216, 1326, 1645, 1786, 1793, 1808
EaeI YGGCCR 1 cut(s) 571
EagI CGGCCG 1 cut(s) 571
Eam1104I CTCTTC 2 cut(s) 71, 82
EarI CTCTTC 2 cut(s) 71, 82
EciI GGCGGA 1 cut(s) 1025
Ecl136II GAGCTC 1 cut(s) 464
EclXI CGGCCG 1 cut(s) 571
Eco130I CCWWGG 1 cut(s) 1302
Eco147I AGGCCT 1 cut(s) 1580
Eco24I GRGCYC 2 cut(s) 466, 812
Eco32I GATATC 1 cut(s) 1102
Eco47I GGWCC 5 cut(s) 345, 576, 997, 1306, 1749
Eco47III AGCGCT 1 cut(s) 619
Eco52I CGGCCG 1 cut(s) 571
Eco53kI GAGCTC 1 cut(s) 464
Eco81I CCTNAGG 1 cut(s) 1106
Eco88I CYCGRG 1 cut(s) 556
EcoICRI GAGCTC 1 cut(s) 464
EcoO109I RGGNCCY 1 cut(s) 997
EcoRI GAATTC 1 cut(s) 142
EcoRII CCWGG 2 cut(s) 433, 1662
EcoRV GATATC 1 cut(s) 1102
EcoT14I CCWWGG 1 cut(s) 1302
EcoT22I ATGCAT 1 cut(s) 1677
EcoT38I GRGCYC 2 cut(s) 466, 812
ErhI CCWWGG 1 cut(s) 1302
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 5 cut(s) 880, 1184, 1274, 1291, 1679
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 1 cut(s) 624
FatI CATG 5 cut(s) 876, 1180, 1270, 1287, 1675
FauI CCCGC 1 cut(s) 111
FbaI TGATCA 1 cut(s) 1326
FblI GTMKAC 1 cut(s) 1194
FokI GGATG 2 cut(s) 260, 1019
FriOI GRGCYC 2 cut(s) 466, 812
FspBI CTAG 5 cut(s) 245, 489, 801, 981, 1151
GlaI GCGC 7 cut(s) 74, 266, 323, 332, 395, 569, 619
GsaI CCCAGC 1 cut(s) 930
GsuI CTGGAG 2 cut(s) 569, 888
HaeII RGCGCY 2 cut(s) 325, 621
HaeIII GGCC 5 cut(s) 297, 561, 573, 1433, 1580
HapII CCGG 9 cut(s) 277, 294, 328, 343, 467, 574, 786, 1309, 1429
HhaI GCGC 7 cut(s) 75, 267, 324, 333, 396, 570, 620
Hin1II CATG 5 cut(s) 880, 1184, 1274, 1291, 1679
Hin6I GCGC 7 cut(s) 73, 265, 322, 331, 394, 568, 618
HinP1I GCGC 7 cut(s) 73, 265, 322, 331, 394, 568, 618
HindIII AAGCTT 3 cut(s) 40, 1529, 1816
HinfI GANTC 5 cut(s) 307, 377, 452, 596, 944
HpaII CCGG 9 cut(s) 277, 294, 328, 343, 467, 574, 786, 1309, 1429
HphI GGTGA 6 cut(s) 181, 378, 575, 929, 1336, 1655
Hpy166II GTNNAC 2 cut(s) 1195, 1700
Hpy188I TCNGA 5 cut(s) 359, 829, 1209, 1624, 1729
Hpy188III TCNNGA 7 cut(s) 139, 449, 489, 786, 867, 1069, 1468
Hpy8I GTNNAC 2 cut(s) 1195, 1700
HpyAV CCTTC 9 cut(s) 259, 396, 572, 719, 866, 1120, 1534, 1543, 1570
HpyCH4III ACNGT 5 cut(s) 537, 647, 750, 820, 1721
HpyCH4IV ACGT 4 cut(s) 613, 1369, 1535, 1588
HpyF10VI GCNNNNNNNGC 7 cut(s) 286, 328, 330, 567, 1000, 1577, 1761
HpyF3I CTNAG 3 cut(s) 38, 1106, 1790
HpySE526I ACGT 4 cut(s) 613, 1369, 1535, 1588
Hsp92II CATG 5 cut(s) 880, 1184, 1274, 1291, 1679
HspAI GCGC 7 cut(s) 73, 265, 322, 331, 394, 568, 618
Kpn2I TCCGGA 1 cut(s) 785
KroI GCCGGC 1 cut(s) 327
KroNI GCCGGC 1 cut(s) 329
Ksp22I TGATCA 1 cut(s) 1326
Kzo9I GATC 7 cut(s) 655, 1216, 1326, 1645, 1786, 1793, 1808
LguI GCTCTTC 1 cut(s) 82
LmnI GCTCC 4 cut(s) 469, 634, 706, 1684
Lsp1109I GCAGC 8 cut(s) 273, 331, 607, 989, 1063, 1328, 1555, 1649
LweI GCATC 2 cut(s) 350, 1167
MaeI CTAG 5 cut(s) 245, 489, 801, 981, 1151
MaeII ACGT 4 cut(s) 613, 1369, 1535, 1588
MaeIII GTNAC 8 cut(s) 14, 581, 814, 879, 1060, 1370, 1437, 1584
MalI GATC 7 cut(s) 657, 1218, 1328, 1647, 1788, 1795, 1810
MboI GATC 7 cut(s) 655, 1216, 1326, 1645, 1786, 1793, 1808
MflI RGATCY 1 cut(s) 1786
MhlI GDGCHC 4 cut(s) 466, 639, 717, 812
MluCI AATT 7 cut(s) 120, 134, 142, 155, 268, 517, 693
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 1 cut(s) 724
Mph1103I ATGCAT 1 cut(s) 1677
MreI CGCCGGCG 1 cut(s) 327
MroI TCCGGA 1 cut(s) 785
MroNI GCCGGC 1 cut(s) 327
MseI TTAA 4 cut(s) 1089, 1293, 1343, 1837
MslI CAYNNNNRTG 2 cut(s) 10, 1450
MspA1I CMGCKG 1 cut(s) 1640
MspI CCGG 9 cut(s) 277, 294, 328, 343, 467, 574, 786, 1309, 1429
MspR9I CCNGG 3 cut(s) 435, 1430, 1664
Mva1269I GAATGC 1 cut(s) 507
MvaI CCWGG 2 cut(s) 435, 1664
MvnI CGCG 6 cut(s) 73, 265, 333, 350, 570, 626
MwoI GCNNNNNNNGC 7 cut(s) 286, 328, 330, 567, 1000, 1577, 1761
NaeI GCCGGC 1 cut(s) 329
NciI CCSGG 1 cut(s) 1430
NdeII GATC 7 cut(s) 655, 1216, 1326, 1645, 1786, 1793, 1808
NgoMIV GCCGGC 1 cut(s) 327
NlaIII CATG 5 cut(s) 880, 1184, 1274, 1291, 1679
NlaIV GGNNCC 2 cut(s) 6, 531
NmeAIII GCCGAG 1 cut(s) 619
NmuCI GTSAC 3 cut(s) 581, 879, 1584
NsiI ATGCAT 1 cut(s) 1677
NspI RCATGY 3 cut(s) 880, 1274, 1679
NspV TTCGAA 1 cut(s) 400
OliI CACNNNNGTG 1 cut(s) 1450
PaeI GCATGC 2 cut(s) 1274, 1679
PceI AGGCCT 1 cut(s) 1580
PciSI GCTCTTC 1 cut(s) 82
PcsI WCGNNNNNNNCGW 1 cut(s) 1375
PctI GAATGC 1 cut(s) 507
PdiI GCCGGC 1 cut(s) 329
PfeI GAWTC 4 cut(s) 307, 377, 452, 944
PleI GAGTC 1 cut(s) 604
PpsI GAGTC 1 cut(s) 604
Ppu21I YACGTR 1 cut(s) 1589
PpuMI RGGWCCY 1 cut(s) 997
PshBI ATTAAT 1 cut(s) 1343
Psp124BI GAGCTC 1 cut(s) 466
Psp5II RGGWCCY 1 cut(s) 997
Psp6I CCWGG 2 cut(s) 433, 1662
PspFI CCCAGC 1 cut(s) 926
PspGI CCWGG 2 cut(s) 433, 1662
PspN4I GGNNCC 2 cut(s) 6, 531
PspPI GGNCC 8 cut(s) 296, 345, 559, 576, 997, 1306, 1431, 1749
PspPPI RGGWCCY 1 cut(s) 997
PstI CTGCAG 1 cut(s) 1786
PstNI CAGNNNCTG 1 cut(s) 1000
PsuI RGATCY 1 cut(s) 1786
PvuII CAGCTG 1 cut(s) 1640
RsaI GTAC 5 cut(s) 554, 1226, 1349, 1427, 1554
RsaNI GTAC 5 cut(s) 553, 1225, 1348, 1426, 1553
RseI CAYNNNNRTG 2 cut(s) 10, 1450
Rsr2I CGGWCCG 1 cut(s) 345
RsrII CGGWCCG 1 cut(s) 345
SacI GAGCTC 1 cut(s) 466
SapI GCTCTTC 1 cut(s) 82
SaqAI TTAA 4 cut(s) 1089, 1293, 1343, 1837
Sau3AI GATC 7 cut(s) 655, 1216, 1326, 1645, 1786, 1793, 1808
Sau96I GGNCC 8 cut(s) 296, 345, 559, 576, 997, 1306, 1431, 1749
ScaI AGTACT 1 cut(s) 554
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 3 cut(s) 435, 1430, 1664
SduI GDGCHC 4 cut(s) 466, 639, 717, 812
SfaNI GCATC 2 cut(s) 350, 1167
SfcI CTRYAG 1 cut(s) 1782
SfuI TTCGAA 1 cut(s) 400
SgrAI CRCCGGYG 1 cut(s) 327
SinI GGWCC 5 cut(s) 345, 576, 997, 1306, 1749
SmiMI CAYNNNNRTG 2 cut(s) 10, 1450
SphI GCATGC 2 cut(s) 1274, 1679
Sse9I AATT 7 cut(s) 120, 134, 142, 155, 268, 517, 693
SseBI AGGCCT 1 cut(s) 1580
SsiI CCGC 6 cut(s) 104, 325, 348, 570, 624, 1036
SspMI CTAG 5 cut(s) 245, 489, 801, 981, 1151
SstI GAGCTC 1 cut(s) 466
StuI AGGCCT 1 cut(s) 1580
StyD4I CCNGG 3 cut(s) 433, 1428, 1662
StyI CCWWGG 1 cut(s) 1302
TaaI ACNGT 5 cut(s) 537, 647, 750, 820, 1721
TaiI ACGT 4 cut(s) 616, 1372, 1538, 1591
TaqI TCGA 5 cut(s) 400, 473, 662, 887, 1094
TasI AATT 7 cut(s) 120, 134, 142, 155, 268, 517, 693
TatI WGTACW 2 cut(s) 552, 1347
TauI GCSGC 3 cut(s) 327, 573, 626
TfiI GAWTC 4 cut(s) 307, 377, 452, 944
Tru1I TTAA 4 cut(s) 1089, 1293, 1343, 1837
Tru9I TTAA 4 cut(s) 1089, 1293, 1343, 1837
TscAI CASTG 5 cut(s) 396, 540, 825, 1455, 1639
TseFI GTSAC 3 cut(s) 581, 879, 1584
TseI GCWGC 8 cut(s) 286, 319, 620, 977, 1076, 1316, 1568, 1637
Tsp45I GTSAC 3 cut(s) 581, 879, 1584
TspDTI ATGAA 3 cut(s) 1021, 1169, 1589
TspGWI ACGGA 2 cut(s) 300, 1762
TspRI CASTG 5 cut(s) 396, 540, 825, 1455, 1639
VpaK11BI GGWCC 5 cut(s) 345, 576, 997, 1306, 1749
VspI ATTAAT 1 cut(s) 1343
XapI RAATTY 2 cut(s) 142, 155
XbaI TCTAGA 1 cut(s) 488
XceI RCATGY 3 cut(s) 880, 1274, 1679
XcmI CCANNNNNNNNNTGG 1 cut(s) 1449
XmiI GTMKAC 1 cut(s) 1194
XspI CTAG 5 cut(s) 245, 489, 801, 981, 1151
ZrmI AGTACT 1 cut(s) 554
Zsp2I ATGCAT 1 cut(s) 1677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.