Rroxscaffold_4G00284730

RING finger and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003923
Physical Location & Seq
Reverse (-)
6293484 .. 6298300
4817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284730.1

Sequence Viewer

Length: 1314 bp
ATGGAGGAATCGGCCGGCAATGGGAGCAGTGCGAGCAGTGGAGGGAACAGTAGTGGTGGTGGTGGTGAGGATCGTGCGACGTCGTTTGGCTCTATGCAATTCCCGGCGCTGCGATTCTTCCAGTCGCCGCTTTCTTTTCTGTTGGATTACTCCGGCATACTCTCGCCTACCTCGTCGTCCTCGCGCCACGAGAGCGACGCTCCGGTGGTCAACGCGGGCGTCGTCGCCGATTCGCGGCCGCATTCCCCTGCGGCCTGCACCAGCGGCTCTAGCAACGCCGGAGAGGTTTCGATTCGGATTATCGGCGCCGGGGAGCAGGAGGACCGCGGGAATAGTAACGAAACGACGGCGTTGCAAGGGCAGCGTGGAGAGGAGAATGGAGGGGTGGAAGGGGTGGATAATCAGGCCGGGATGGATGAGAGAGTCCCTTGGTGGCTCCGTCGTCGCCGGAGCATGAGGCCGGGGAGGCTGGGAATGGCGTGGAAGAGAATTCTAGGGATTCGGCTTACCAGAGATATGATATTCAACAGGCATGCGAATGCAAGAGGCTGTTTCTTTGTCACTATATGGATATCAGCTGTCATGTATAGGTCAAATGACATTGTTAGGAAGCAGACGGCTCTAAAGGGAGACAGGAAAATCTCTTGTCTTGTGGGCATTGCTGTTGTTTTTGTGCTTCATGTGGTTGGTGTTTACTGGTGGTATCGAAATGATGATCTTTTATACCCGCTAGCAATGCTTCCTCCAAAAGCCATACCACCATTCTGGCATGCAATATTCATCATTTTGGCAACGATGGCTTTCAAGTGTTTGTTGCTTGTATACTACAAGAATGGAAGAGGGCATAACTATCGTCGACAGGGTCAAATGTTAACCGTAGTTGAGTATTCGCTGCTGCTGTACCGTGCCTTATTACCAGCACCTGTTTGGTACAGATTCTTCCTGAACAAAGAATATGGAAGCCTCTTCTCCTCACTTATTACAGGATTATACTTGACTTTTAAGCTAACATCTGTTGTTGAGAAGGTCCAATCCTTTTTTGCTTCTATAAGGGCATTATCACGAAAAGATATCCATTATGGGTCCCACGCAACATTGGAACAGGTAAATGCAGCAGGAGACCTTTGTGCCATTTGCCAGGAGAAGATGCATGCTCCTATCCTGTTGCGCTGCAAACACATCTTCTGCGAGGACTGTGTTTCCGAATGGTTTGAGAGAGAAAGAACTTGCCCTCTATGCCGGGCAGTGGTAAAACCTGCAGATCTGCGAACGTTTGGGGATGGATCAACAAGTTTATTTTTCCAGTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

437

Amino Acids

48.72

Weight (kDa)

9.15

Isoelectric Point (pI)

49.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 373 - 414 5.3e-06 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 374 - 417 4.3e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 375 - 414 1.7e-10 Ring finger domain
zf-C3HC4 PF00097 376 - 413 2.9e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 376 - 411 7.2e-08 RING-type zinc-finger
zf-C3HC4_2 PF13923 376 - 413 5.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 376 - 414 2.5e-07 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 83
Acc36I ACCTGC 1 cut(s) 1264
AccB1I GGYRCC 1 cut(s) 305
AccI GTMKAC 2 cut(s) 822, 856
AccII CGCG 4 cut(s) 184, 215, 235, 327
AciI CCGC 9 cut(s) 128, 215, 235, 239, 251, 264, 325, 327, 728
AclI AACGTT 1 cut(s) 1271
AclWI GGATC 2 cut(s) 78, 1291
AcoI YGGCCR 2 cut(s) 12, 236
AcsI RAATTY 1 cut(s) 489
AcyI GRCGYC 3 cut(s) 80, 219, 306
AfaI GTAC 2 cut(s) 902, 932
AfiI CCNNNNNNNGG 3 cut(s) 21, 234, 1246
AgsI TTSAA 2 cut(s) 526, 805
AjnI CCWGG 1 cut(s) 1137
AluBI AGCT 2 cut(s) 578, 1006
AluI AGCT 2 cut(s) 578, 1006
Alw26I GTCTC 2 cut(s) 624, 1113
AlwI GGATC 2 cut(s) 78, 1291
AlwNI CAGNNNCTG 1 cut(s) 923
AoxI GGCC 5 cut(s) 12, 236, 252, 405, 458
ApeKI GCWGC 6 cut(s) 109, 361, 892, 895, 1112, 1170
ApoI RAATTY 1 cut(s) 489
AspLEI GCGC 4 cut(s) 109, 186, 308, 1170
AspS9I GGNCC 3 cut(s) 322, 1027, 1083
AsuC2I CCSGG 5 cut(s) 104, 310, 409, 462, 1241
AsuHPI GGTGA 1 cut(s) 77
AsuNHI GCTAGC 1 cut(s) 730
AvaII GGWCC 3 cut(s) 322, 1027, 1083
BanI GGYRCC 1 cut(s) 305
BarI GAAGNNNNNNTAC 2 cut(s) 923, 955
BauI CACGAG 1 cut(s) 188
BbvI GCAGC 6 cut(s) 96, 373, 879, 882, 1124, 1157
BccI CCATC 3 cut(s) 406, 790, 1274
BceAI ACGGC 2 cut(s) 363, 633
BcgI CGANNNNNNTGC 4 cut(s) 334, 368, 833, 867
BciT130I CCWGG 1 cut(s) 1139
BcnI CCSGG 5 cut(s) 104, 310, 409, 462, 1241
BcoDI GTCTC 2 cut(s) 624, 1113
BfaI CTAG 3 cut(s) 270, 494, 731
BfmI CTRYAG 1 cut(s) 1257
BfoI RGCGCY 2 cut(s) 110, 309
BfuAI ACCTGC 1 cut(s) 1264
BglI GCCNNNNNGGC 1 cut(s) 466
BglII AGATCT 1 cut(s) 1261
Bme1390I CCNGG 6 cut(s) 104, 310, 409, 462, 1139, 1241
Bme18I GGWCC 3 cut(s) 322, 1027, 1083
BmgT120I GGNCC 3 cut(s) 322, 1027, 1083
BmiI GGNNCC 4 cut(s) 307, 437, 1084, 1085
BmrFI CCNGG 6 cut(s) 104, 310, 409, 462, 1139, 1241
BmsI GCATC 1 cut(s) 1137
BmtI GCTAGC 1 cut(s) 734
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BpuMI CCSGG 5 cut(s) 104, 310, 409, 462, 1241
BsaHI GRCGYC 3 cut(s) 80, 219, 306
BsaI GGTCTC 1 cut(s) 1113
BsaJI CCNNGG 4 cut(s) 309, 325, 428, 461
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 234, 1246
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 3 cut(s) 121, 701, 1303
Bse3DI GCAATG 3 cut(s) 25, 657, 741
BseBI CCWGG 1 cut(s) 1139
BseDI CCNNGG 4 cut(s) 309, 325, 428, 461
BseGI GGATG 3 cut(s) 417, 421, 1285
BseLI CCNNNNNNNGG 3 cut(s) 21, 234, 1246
BseMI GCAATG 3 cut(s) 25, 657, 741
BseNI ACTGG 3 cut(s) 121, 701, 1303
BseRI GAGGAG 2 cut(s) 386, 961
BseX3I CGGCCG 2 cut(s) 12, 236
BseXI GCAGC 6 cut(s) 96, 373, 879, 882, 1124, 1157
BseYI CCCAGC 1 cut(s) 469
BsgI GTGCAG 1 cut(s) 241
Bsh1236I CGCG 4 cut(s) 184, 215, 235, 327
Bsh1285I CGRYCG 2 cut(s) 15, 239
BshFI GGCC 5 cut(s) 14, 238, 254, 407, 460
BshNI GGYRCC 1 cut(s) 305
BsiEI CGRYCG 2 cut(s) 15, 239
BslFI GGGAC 2 cut(s) 410, 1069
BslI CCNNNNNNNGG 3 cut(s) 21, 234, 1246
BsmAI GTCTC 2 cut(s) 624, 1113
BsmFI GGGAC 2 cut(s) 410, 1069
BsmI GAATGC 2 cut(s) 241, 544
BsnI GGCC 5 cut(s) 14, 238, 254, 407, 460
Bso31I GGTCTC 1 cut(s) 1113
Bsp143I GATC 4 cut(s) 70, 715, 1261, 1283
BspACI CCGC 9 cut(s) 128, 215, 235, 239, 251, 264, 325, 327, 728
BspANI GGCC 5 cut(s) 14, 238, 254, 407, 460
BspFNI CGCG 4 cut(s) 184, 215, 235, 327
BspLI GGNNCC 4 cut(s) 307, 437, 1084, 1085
BspMAI CTGCAG 1 cut(s) 1261
BspMI ACCTGC 1 cut(s) 1264
BspOI GCTAGC 1 cut(s) 734
BspPI GGATC 2 cut(s) 78, 1291
BspT107I GGYRCC 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 1113
BsrDI GCAATG 3 cut(s) 25, 657, 741
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 3 cut(s) 121, 701, 1303
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 4 cut(s) 309, 325, 428, 461
BssMI GATC 4 cut(s) 70, 715, 1261, 1283
BssNAI GTATAC 1 cut(s) 823
BssNI GRCGYC 3 cut(s) 80, 219, 306
BssSI CACGAG 1 cut(s) 188
BssT1I CCWWGG 1 cut(s) 428
Bst1107I GTATAC 1 cut(s) 823
Bst2BI CACGAG 1 cut(s) 188
Bst2UI CCWGG 1 cut(s) 1139
Bst4CI ACNGT 4 cut(s) 50, 877, 905, 1196
Bst6I CTCTTC 3 cut(s) 479, 832, 971
BstACI GRCGYC 3 cut(s) 80, 219, 306
BstC8I GCNNGC 8 cut(s) 16, 34, 217, 256, 534, 732, 771, 1152
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 3 cut(s) 417, 421, 1285
BstFNI CGCG 4 cut(s) 184, 215, 235, 327
BstH2I RGCGCY 2 cut(s) 110, 309
BstHHI GCGC 4 cut(s) 109, 186, 308, 1170
BstKTI GATC 4 cut(s) 73, 718, 1264, 1286
BstMAI GTCTC 2 cut(s) 624, 1113
BstMBI GATC 4 cut(s) 70, 715, 1261, 1283
BstMCI CGRYCG 2 cut(s) 15, 239
BstNI CCWGG 1 cut(s) 1139
BstNSI RCATGY 3 cut(s) 536, 773, 1154
BstSCI CCNGG 6 cut(s) 102, 308, 407, 460, 1137, 1239
BstSFI CTRYAG 1 cut(s) 1257
BstUI CGCG 4 cut(s) 184, 215, 235, 327
BstV1I GCAGC 6 cut(s) 96, 373, 879, 882, 1124, 1157
BstX2I RGATCY 1 cut(s) 1261
BstXI CCANNNNNNTGG 1 cut(s) 765
BstYI RGATCY 1 cut(s) 1261
BstZ17I GTATAC 1 cut(s) 823
BstZI CGGCCG 2 cut(s) 12, 236
BsuRI GGCC 5 cut(s) 14, 238, 254, 407, 460
BtgI CCRYGG 1 cut(s) 325
BtsCI GGATG 3 cut(s) 417, 421, 1285
BtsI GCAGTG 3 cut(s) 34, 43, 1251
BtsIMutI CAGTG 3 cut(s) 34, 43, 1251
BveI ACCTGC 1 cut(s) 1264
Cac8I GCNNGC 8 cut(s) 16, 34, 217, 256, 534, 732, 771, 1152
CaiI CAGNNNCTG 1 cut(s) 923
CciNI GCGGCCGC 1 cut(s) 236
CfoI GCGC 4 cut(s) 109, 186, 308, 1170
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 3 cut(s) 322, 1027, 1083
Cfr42I CCGCGG 1 cut(s) 328
CseI GACGC 2 cut(s) 206, 208
Csp6I GTAC 2 cut(s) 901, 931
CviAII CATG 6 cut(s) 454, 533, 583, 680, 770, 1151
CviQI GTAC 2 cut(s) 901, 931
DinI GGCGCC 1 cut(s) 307
DpnI GATC 4 cut(s) 72, 717, 1263, 1285
DpnII GATC 4 cut(s) 70, 715, 1261, 1283
EaeI YGGCCR 2 cut(s) 12, 236
EagI CGGCCG 2 cut(s) 12, 236
Eam1104I CTCTTC 3 cut(s) 479, 832, 971
EarI CTCTTC 3 cut(s) 479, 832, 971
EclXI CGGCCG 2 cut(s) 12, 236
Eco130I CCWWGG 1 cut(s) 428
Eco31I GGTCTC 1 cut(s) 1113
Eco32I GATATC 2 cut(s) 573, 1072
Eco47I GGWCC 3 cut(s) 322, 1027, 1083
Eco52I CGGCCG 2 cut(s) 12, 236
EcoO109I RGGNCCY 1 cut(s) 1083
EcoRI GAATTC 1 cut(s) 489
EcoRII CCWGG 1 cut(s) 1137
EcoRV GATATC 2 cut(s) 573, 1072
EcoT14I CCWWGG 1 cut(s) 428
EcoT22I ATGCAT 1 cut(s) 1152
EgeI GGCGCC 1 cut(s) 307
EheI GGCGCC 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 428
FaeI CATG 6 cut(s) 457, 536, 586, 683, 773, 1154
FaqI GGGAC 2 cut(s) 410, 1069
FatI CATG 6 cut(s) 453, 532, 582, 679, 769, 1150
FauI CCCGC 3 cut(s) 208, 320, 735
FblI GTMKAC 2 cut(s) 822, 856
FokI GGATG 3 cut(s) 424, 428, 1292
FspBI CTAG 3 cut(s) 270, 494, 731
GlaI GCGC 4 cut(s) 108, 185, 307, 1169
GsaI CCCAGC 1 cut(s) 473
HaeII RGCGCY 2 cut(s) 110, 309
HaeIII GGCC 5 cut(s) 14, 238, 254, 407, 460
HgaI GACGC 2 cut(s) 206, 208
HhaI GCGC 4 cut(s) 109, 186, 308, 1170
Hin1I GRCGYC 3 cut(s) 80, 219, 306
Hin1II CATG 6 cut(s) 457, 536, 586, 683, 773, 1154
Hin6I GCGC 4 cut(s) 107, 184, 306, 1168
HinP1I GCGC 4 cut(s) 107, 184, 306, 1168
HincII GTYRAC 3 cut(s) 211, 857, 873
HindII GTYRAC 3 cut(s) 211, 857, 873
HinfI GANTC 7 cut(s) 8, 114, 230, 292, 423, 499, 936
HpaI GTTAAC 1 cut(s) 873
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 5 cut(s) 211, 694, 823, 857, 873
Hpy188I TCNGA 3 cut(s) 297, 1204, 1313
Hpy188III TCNNGA 2 cut(s) 943, 1062
Hpy8I GTNNAC 5 cut(s) 211, 694, 823, 857, 873
HpyAV CCTTC 2 cut(s) 383, 1018
HpyCH4III ACNGT 4 cut(s) 50, 877, 905, 1196
HpyCH4IV ACGT 2 cut(s) 80, 1271
HpyCH4V TGCA 9 cut(s) 97, 258, 355, 542, 773, 1112, 1150, 1173, 1259
HpySE526I ACGT 2 cut(s) 80, 1271
Hsp92I GRCGYC 3 cut(s) 80, 219, 306
Hsp92II CATG 6 cut(s) 457, 536, 586, 683, 773, 1154
HspAI GCGC 4 cut(s) 107, 184, 306, 1168
KasI GGCGCC 1 cut(s) 305
KflI GGGWCCC 1 cut(s) 1083
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
KspAI GTTAAC 1 cut(s) 873
KspI CCGCGG 1 cut(s) 328
Kzo9I GATC 4 cut(s) 70, 715, 1261, 1283
LmnI GCTCC 6 cut(s) 24, 205, 313, 441, 450, 1159
Lsp1109I GCAGC 6 cut(s) 96, 373, 879, 882, 1124, 1157
LweI GCATC 1 cut(s) 1137
MaeI CTAG 3 cut(s) 270, 494, 731
MaeII ACGT 2 cut(s) 80, 1271
MaeIII GTNAC 2 cut(s) 335, 559
MalI GATC 4 cut(s) 72, 717, 1263, 1285
MboI GATC 4 cut(s) 70, 715, 1261, 1283
MboII GAAGA 8 cut(s) 109, 496, 849, 931, 958, 1156, 1174, 1300
MflI RGATCY 1 cut(s) 1261
MluCI AATT 2 cut(s) 98, 489
Mly113I GGCGCC 1 cut(s) 306
MlyI GAGTC 1 cut(s) 432
MmeI TCCRAC 1 cut(s) 123
Mph1103I ATGCAT 1 cut(s) 1152
MroNI GCCGGC 1 cut(s) 14
MseI TTAA 2 cut(s) 872, 1002
MslI CAYNNNNRTG 1 cut(s) 537
MspA1I CMGCKG 3 cut(s) 264, 327, 578
MspR9I CCNGG 6 cut(s) 104, 310, 409, 462, 1139, 1241
Mva1269I GAATGC 2 cut(s) 241, 544
MvaI CCWGG 1 cut(s) 1139
MvnI CGCG 4 cut(s) 184, 215, 235, 327
NaeI GCCGGC 1 cut(s) 16
NarI GGCGCC 1 cut(s) 306
NciI CCSGG 5 cut(s) 104, 310, 409, 462, 1241
NdeII GATC 4 cut(s) 70, 715, 1261, 1283
NgoMIV GCCGGC 1 cut(s) 14
NheI GCTAGC 1 cut(s) 730
NlaIII CATG 6 cut(s) 457, 536, 586, 683, 773, 1154
NlaIV GGNNCC 4 cut(s) 307, 437, 1084, 1085
NmuCI GTSAC 1 cut(s) 559
NotI GCGGCCGC 1 cut(s) 236
NsiI ATGCAT 1 cut(s) 1152
NspI RCATGY 3 cut(s) 536, 773, 1154
PaeI GCATGC 3 cut(s) 536, 773, 1154
PcsI WCGNNNNNNNCGW 2 cut(s) 170, 219
PctI GAATGC 2 cut(s) 241, 544
PdiI GCCGGC 1 cut(s) 16
PfeI GAWTC 6 cut(s) 8, 114, 230, 292, 499, 936
PflFI GACNNNGTC 1 cut(s) 861
PleI GAGTC 1 cut(s) 431
PluTI GGCGCC 1 cut(s) 309
PpsI GAGTC 1 cut(s) 431
PpuMI RGGWCCY 1 cut(s) 1083
Psp1406I AACGTT 1 cut(s) 1271
Psp5II RGGWCCY 1 cut(s) 1083
Psp6I CCWGG 1 cut(s) 1137
PspFI CCCAGC 1 cut(s) 469
PspGI CCWGG 1 cut(s) 1137
PspN4I GGNNCC 4 cut(s) 307, 437, 1084, 1085
PspPI GGNCC 3 cut(s) 322, 1027, 1083
PspPPI RGGWCCY 1 cut(s) 1083
PstI CTGCAG 1 cut(s) 1261
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 1 cut(s) 1261
PsyI GACNNNGTC 1 cut(s) 861
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 2 cut(s) 902, 932
RsaNI GTAC 2 cut(s) 901, 931
RseI CAYNNNNRTG 1 cut(s) 537
SacII CCGCGG 1 cut(s) 328
SalI GTCGAC 1 cut(s) 855
SaqAI TTAA 2 cut(s) 872, 1002
Sau3AI GATC 4 cut(s) 70, 715, 1261, 1283
Sau96I GGNCC 3 cut(s) 322, 1027, 1083
SchI GAGTC 1 cut(s) 432
ScrFI CCNGG 6 cut(s) 104, 310, 409, 462, 1139, 1241
SfaNI GCATC 1 cut(s) 1137
SfcI CTRYAG 1 cut(s) 1257
SfoI GGCGCC 1 cut(s) 307
Sfr303I CCGCGG 1 cut(s) 328
SgrBI CCGCGG 1 cut(s) 328
SinI GGWCC 3 cut(s) 322, 1027, 1083
SmiMI CAYNNNNRTG 1 cut(s) 537
SphI GCATGC 3 cut(s) 536, 773, 1154
Sse9I AATT 2 cut(s) 98, 489
SsiI CCGC 9 cut(s) 128, 215, 235, 239, 251, 264, 325, 327, 728
SspDI GGCGCC 1 cut(s) 305
SspI AATATT 1 cut(s) 777
SspMI CTAG 3 cut(s) 270, 494, 731
StyD4I CCNGG 6 cut(s) 102, 308, 407, 460, 1137, 1239
StyI CCWWGG 1 cut(s) 428
TaaI ACNGT 4 cut(s) 50, 877, 905, 1196
TaiI ACGT 2 cut(s) 83, 1274
TaqI TCGA 3 cut(s) 290, 706, 856
TasI AATT 2 cut(s) 98, 489
TauI GCSGC 5 cut(s) 130, 238, 241, 254, 267
TfiI GAWTC 6 cut(s) 8, 114, 230, 292, 499, 936
Tru1I TTAA 2 cut(s) 872, 1002
Tru9I TTAA 2 cut(s) 872, 1002
TscAI CASTG 3 cut(s) 34, 43, 1251
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 6 cut(s) 109, 361, 892, 895, 1112, 1170
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 2 cut(s) 668, 769
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 3 cut(s) 34, 43, 1251
Tth111I GACNNNGTC 1 cut(s) 861
VpaK11BI GGWCC 3 cut(s) 322, 1027, 1083
XapI RAATTY 1 cut(s) 489
XceI RCATGY 3 cut(s) 536, 773, 1154
XcmI CCANNNNNNNNNTGG 1 cut(s) 924
XmiI GTMKAC 2 cut(s) 822, 856
XspI CTAG 3 cut(s) 270, 494, 731
ZraI GACGTC 1 cut(s) 81
Zsp2I ATGCAT 1 cut(s) 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.