Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002129

Physical Location & Seq

Genomic Coordinates Reverse (-)

3221493 .. 3224788

Gene Structure 3296 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G10270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGGCGATGGCATCATTCGGGCAGCTAAATCTCGAGGAACCTCCTCCAATCTGGGGATCTCGCAGCGTTGATTGCTTTGAGAAGCTCGAACAAATTGGTGAAGGCACTTACGGTCAAGTTTACATGGCTAAAGAAATCAAAACTGGTGAAATTGTGGCTCTCAAAAAGATACGTATGGACAATGAAAGAGAAGGGTTTCCTATAACAGCTATTAGAGAGATAAAGATTCTGAAGAAGCTTCATCATGAAAATGTCATTCAGCTGAAAGAGATTGTGACTTCACCAGGTCGGGACAGGGATGACCAAGGAAAGCCAGATAATAACAAATACAAGGGTGGCATCTACATGGTTTTTGAGTACATGGATCATGATTTGACTGGACTAGCAGATCGTCCTGGACTGAGATTTACTGTTCCTCAAATTAAGTGTTACATGAAGCAATTGCTTACCGGGCTTCACTATTGTCATGTGAATCAAGTGCTTCACCGTGATATAAAAGGCTCAAATCTCCTTATCGACAATGAGGGAAATTTAAAGCTGGCTGATTTTGGGCTTGCACGGTCGTATTCTCATGATCATACTGGAAATCTTACAAATCGTGTCATCACATTGTGGTATAGGCCCCCTGAATTACTACTTGGGGCTACAAAATATGGCCCAGCAATTGACATGTGGTCGGTTGGTTGCATATTTGCCGAACTTTTGCATGCAAAACCAATCTTACCTGGGAAAAATGAGCAAGAACAATTGAACAAGATATTTGAGCTTTGTGGATCACCTGATGAAAAACTTTGGCCTGGGGTTTCCAAGATGCCTTGGTTCAACAATTTCAAGCCTGCACGGCCCTTGAAGAGGCGTGTAAGAGAGTTCTTCAGACACTTTGATCGGCATGCTCTTGAATTACTGGAGAAAATGTTGGTGCTTGATCCAGCACAGAGAATATCGGCAAAGGATGCTCTTGATGCCGAGTACTTTTGGACTGATCCGTTGCCATGTGACCCAAAGAGTCTGCCCACATATGAATCATCACATGAGTTCCAGACAAAGAAAAAGCGGCAACAGCAGCGCCAAAACGAGGAAGCAGCAAAAAGACAGAAACTGCAGCATCCACCGCTGCAGCACTCTCGCTTACCCCCATTACAACATGGTGGACAGTCTCATGCTGCTCCACATTGGCCTGCAGGTCCAAACCATCCCACTAACAACGCACCACCACAAGTACCTGCTGGACCCAGCCACAACTTCTATGGGAAGCCGCGTGGTCCACCTGGTCCAAACCGCTACCCTCCTAGCGGAAACCAGAGCGGGGGTTATAATCAAAGCCGAGGAGGTTACAGCAGTGGATCATATCCTCCACAAGGACGTGGAGCTCCTTATGTGGCTGGTCCTAGAGGGCCTAGTGGTGGCCCGTACGGGGTTGGACCTCCTAACTACACACAAGGTGGTCAGTATGGTGGCTCTGGTAGCTCGGGAAGAGGGCAGAATCAGAGAAACCAGCAATACGGATGGCAACAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009791 GO:0009908 GO:0009987 GO:0010035 GO:0010467 GO:0016070 GO:0016071 GO:0016301 GO:0016310 GO:0016604 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0034641 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043900 GO:0043903 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050792 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0061458 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0090567 GO:0097472 GO:0099402 GO:0140096 GO:1901360 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

505

Amino Acids

56.73

Weight (kDa)

9.29

Isoelectric Point (pI)

44.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	26 - 325	3.1e-71	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	28 - 253	1.7e-28	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002129)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10270 AT5G64960 AT5G64960
fragaria_vesca	FvH4_1g20740 FvH4_1g20740 FvH4_1g20740 FvH4_1g20740 FvH4_4g11370 FvH4_4g11370 FvH4_4g11370
malus_domestica	MD04G1037600.v1.1 MD15G1318300.v1.1
prunus_persica	Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.6G187600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0113361 RchiOBHm_Chr2g0113381 RchiOBHm_Chr4g0407881
rosa_laevigata	RLG00000008692 RLG00000018058
rosa_multiflora	Rmu_sc0002073.1_g000029 Rmu_sc0007621.1_g000001 Rmu_sc0011967.1_g000006 Rmu_sc0029075.1_g000002 Rmu_sc0029867.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129320 Rroxscaffold_2G00129850 Rroxscaffold_5G00352420
rosa_rugosa	Rorug02G0186500 Rorug04G0081800 Rorug04G0081900
rosa_samantha	Rh2AG241100 Rh2BG255300 Rh2CG245400 Rh2DG249100 Rh4AG147000 Rh4BG144500 Rh4CG153800 Rh4DG141000
rosa_wichuraiana	Rw2G018830 Rw4G012070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1314
Acc36I	ACCTGC	2 cut(s)	1172, 1231
AccBSI	CCGCTC	1 cut(s)	1305
AccII	CGCG	1 cut(s)	1258
AciI	CCGC	6 cut(s)	1054, 1112, 1256, 1279, 1293, 1305
AclWI	GGATC	6 cut(s)	64, 372, 781, 920, 977, 1351
AcsI	RAATTY	1 cut(s)	529
AcuI	CTGAAG	2 cut(s)	251, 856
AdeI	CACNNNGTG	2 cut(s)	612, 1442
AfaI	GTAC	4 cut(s)	359, 971, 1221, 1412
AfiI	CCNNNNNNNGG	8 cut(s)	53, 852, 1075, 1292, 1306, 1358, 1403, 1414
AflIII	ACRYGT	1 cut(s)	669
AgsI	TTSAA	5 cut(s)	751, 823, 832, 850, 899
AhdI	GACNNNNNGTC	1 cut(s)	673
AjiI	CACGTC	1 cut(s)	1364
AjnI	CCWGG	5 cut(s)	283, 394, 724, 796, 1267
AjuI	GAANNNNNNNTTGG	2 cut(s)	621, 653
AluBI	AGCT	9 cut(s)	25, 85, 209, 238, 262, 538, 766, 1370, 1467
AluI	AGCT	9 cut(s)	25, 85, 209, 238, 262, 538, 766, 1370, 1467
Alw21I	GWGCWC	1 cut(s)	1372
Alw26I	GTCTC	1 cut(s)	1161
AlwI	GGATC	6 cut(s)	64, 372, 781, 920, 977, 1351
AlwNI	CAGNNNCTG	1 cut(s)	1099
Ama87I	CYCGRG	2 cut(s)	32, 1468
AoxI	GGCC	7 cut(s)	620, 655, 794, 842, 1175, 1394, 1405
ApeKI	GCWGC	8 cut(s)	22, 63, 1063, 1082, 1102, 1114, 1117, 1163
ApoI	RAATTY	1 cut(s)	529
Asp700I	GAANNNNTTC	1 cut(s)	195
AspLEI	GCGC	1 cut(s)	1068
AsuC2I	CCSGG	1 cut(s)	451
AsuHPI	GGTGA	5 cut(s)	110, 158, 273, 476, 768
AvaI	CYCGRG	2 cut(s)	32, 1468
AvaII	GGWCC	6 cut(s)	1184, 1229, 1262, 1271, 1385, 1421
BanII	GRGCYC	1 cut(s)	1372
Bbv12I	GWGCWC	1 cut(s)	1372
BbvI	GCAGC	8 cut(s)	34, 75, 1075, 1094, 1101, 1114, 1129, 1150
BccI	CCATC	2 cut(s)	1200, 1500
BceAI	ACGGC	1 cut(s)	857
BcgI	CGANNNNNNTGC	2 cut(s)	1106, 1140
BciT130I	CCWGG	5 cut(s)	285, 396, 726, 798, 1269
BclI	TGATCA	1 cut(s)	574
BcnI	CCSGG	1 cut(s)	451
BcoDI	GTCTC	1 cut(s)	1161
BfaI	CTAG	4 cut(s)	383, 1290, 1389, 1398
BfmI	CTRYAG	3 cut(s)	1100, 1115, 1179
BfoI	RGCGCY	1 cut(s)	1069
BfuAI	ACCTGC	2 cut(s)	1172, 1231
BglI	GCCNNNNNGGC	1 cut(s)	841
BmcAI	AGTACT	1 cut(s)	971
Bme1390I	CCNGG	6 cut(s)	285, 396, 451, 726, 798, 1269
Bme18I	GGWCC	6 cut(s)	1184, 1229, 1262, 1271, 1385, 1421
BmeRI	GACNNNNNGTC	1 cut(s)	673
BmeT110I	CYCGRG	2 cut(s)	32, 1468
BmgBI	CACGTC	1 cut(s)	1364
BmiI	GGNNCC	3 cut(s)	39, 623, 1231
BmrFI	CCNGG	6 cut(s)	285, 396, 451, 726, 798, 1269
BmsI	GCATC	6 cut(s)	20, 348, 801, 943, 952, 1114
BpmI	CTGGAG	1 cut(s)	926
BpuMI	CCSGG	1 cut(s)	451
BsaAI	YACGTR	1 cut(s)	173
BsaJI	CCNNGG	5 cut(s)	304, 725, 797, 815, 1324
BsaXI	ACNNNNNCTCC	2 cut(s)	899, 929
Bsc4I	CCNNNNNNNGG	8 cut(s)	53, 852, 1075, 1292, 1306, 1358, 1403, 1414
Bse1I	ACTGG	4 cut(s)	148, 382, 586, 909
BseBI	CCWGG	5 cut(s)	285, 396, 726, 798, 1269
BseDI	CCNNGG	5 cut(s)	304, 725, 797, 815, 1324
BseGI	GGATG	5 cut(s)	304, 958, 1105, 1192, 1511
BseLI	CCNNNNNNNGG	8 cut(s)	53, 852, 1075, 1292, 1306, 1358, 1403, 1414
BseMII	CTCAG	1 cut(s)	392
BseNI	ACTGG	4 cut(s)	148, 382, 586, 909
BseRI	GAGGAG	2 cut(s)	33, 1341
BseXI	GCAGC	8 cut(s)	34, 75, 1075, 1094, 1101, 1114, 1129, 1150
BseYI	CCCAGC	2 cut(s)	658, 1232
BsgI	GTGCAG	1 cut(s)	822
Bsh1236I	CGCG	1 cut(s)	1258
Bsh1285I	CGRYCG	1 cut(s)	563
BshFI	GGCC	7 cut(s)	622, 657, 796, 844, 1177, 1396, 1407
BsiEI	CGRYCG	1 cut(s)	563
BsiHKAI	GWGCWC	1 cut(s)	1372
BsiHKCI	CYCGRG	2 cut(s)	32, 1468
BsiSI	CCGG	1 cut(s)	450
BsiWI	CGTACG	1 cut(s)	1410
BslFI	GGGAC	1 cut(s)	305
BslI	CCNNNNNNNGG	8 cut(s)	53, 852, 1075, 1292, 1306, 1358, 1403, 1414
BsmAI	GTCTC	1 cut(s)	1161
BsmFI	GGGAC	1 cut(s)	305
BsnI	GGCC	7 cut(s)	622, 657, 796, 844, 1177, 1396, 1407
BsoBI	CYCGRG	2 cut(s)	32, 1468
Bsp1286I	GDGCHC	1 cut(s)	1372
Bsp143I	GATC	9 cut(s)	56, 364, 388, 574, 773, 883, 925, 982, 1343
BspACI	CCGC	6 cut(s)	1054, 1112, 1256, 1279, 1293, 1305
BspANI	GGCC	7 cut(s)	622, 657, 796, 844, 1177, 1396, 1407
BspCNI	CTCAG	1 cut(s)	393
BspFNI	CGCG	1 cut(s)	1258
BspHI	TCATGA	3 cut(s)	244, 367, 571
BspLI	GGNNCC	3 cut(s)	39, 623, 1231
BspMAI	CTGCAG	3 cut(s)	1104, 1119, 1183
BspMI	ACCTGC	2 cut(s)	1172, 1231
BspPI	GGATC	6 cut(s)	64, 372, 781, 920, 977, 1351
BsrBI	CCGCTC	1 cut(s)	1305
BsrI	ACTGG	4 cut(s)	148, 382, 586, 909
BssECI	CCNNGG	5 cut(s)	304, 725, 797, 815, 1324
BssMI	GATC	9 cut(s)	56, 364, 388, 574, 773, 883, 925, 982, 1343
BssT1I	CCWWGG	2 cut(s)	304, 815
Bst2UI	CCWGG	5 cut(s)	285, 396, 726, 798, 1269
Bst4CI	ACNGT	6 cut(s)	113, 412, 488, 561, 1155, 1515
Bst6I	CTCTTC	2 cut(s)	845, 1468
BstAPI	GCANNNNNTGC	1 cut(s)	953
BstBAI	YACGTR	1 cut(s)	173
BstC8I	GCNNGC	6 cut(s)	540, 555, 708, 837, 891, 1179
BstDEI	CTNAG	1 cut(s)	401
BstENI	CCTNNNNNAGG	2 cut(s)	850, 1356
BstF5I	GGATG	5 cut(s)	304, 958, 1105, 1192, 1511
BstFNI	CGCG	1 cut(s)	1258
BstH2I	RGCGCY	1 cut(s)	1069
BstHHI	GCGC	1 cut(s)	1068
BstKTI	GATC	9 cut(s)	59, 367, 391, 577, 776, 886, 928, 985, 1346
BstMAI	GTCTC	1 cut(s)	1161
BstMBI	GATC	9 cut(s)	56, 364, 388, 574, 773, 883, 925, 982, 1343
BstMCI	CGRYCG	1 cut(s)	563
BstMWI	GCNNNNNNNGC	9 cut(s)	72, 451, 841, 953, 962, 1060, 1063, 1111, 1464
BstNI	CCWGG	5 cut(s)	285, 396, 726, 798, 1269
BstNSI	RCATGY	3 cut(s)	673, 710, 893
BstSCI	CCNGG	6 cut(s)	283, 394, 449, 724, 796, 1267
BstSFI	CTRYAG	3 cut(s)	1100, 1115, 1179
BstSNI	TACGTA	1 cut(s)	173
BstUI	CGCG	1 cut(s)	1258
BstV1I	GCAGC	8 cut(s)	34, 75, 1075, 1094, 1101, 1114, 1129, 1150
BstX2I	RGATCY	1 cut(s)	56
BstYI	RGATCY	1 cut(s)	56
BsuRI	GGCC	7 cut(s)	622, 657, 796, 844, 1177, 1396, 1407
BtgZI	GCGATG	1 cut(s)	20
BtrI	CACGTC	1 cut(s)	1364
BtsCI	GGATG	5 cut(s)	304, 958, 1105, 1192, 1511
BtsI	GCAGTG	1 cut(s)	1345
BtsIMutI	CAGTG	1 cut(s)	1345
BveI	ACCTGC	2 cut(s)	1172, 1231
Cac8I	GCNNGC	6 cut(s)	540, 555, 708, 837, 891, 1179
CaiI	CAGNNNCTG	1 cut(s)	1099
CciI	TCATGA	3 cut(s)	244, 367, 571
CfoI	GCGC	1 cut(s)	1068
CsiI	ACCWGGT	2 cut(s)	283, 1267
Csp6I	GTAC	4 cut(s)	358, 970, 1220, 1411
CviQI	GTAC	4 cut(s)	358, 970, 1220, 1411
DdeI	CTNAG	1 cut(s)	401
DpnI	GATC	9 cut(s)	58, 366, 390, 576, 775, 885, 927, 984, 1345
DpnII	GATC	9 cut(s)	56, 364, 388, 574, 773, 883, 925, 982, 1343
DraI	TTTAAA	1 cut(s)	534
DraIII	CACNNNGTG	2 cut(s)	612, 1442
DriI	GACNNNNNGTC	1 cut(s)	673
Eam1104I	CTCTTC	2 cut(s)	845, 1468
Eam1105I	GACNNNNNGTC	1 cut(s)	673
EarI	CTCTTC	2 cut(s)	845, 1468
Ecl136II	GAGCTC	1 cut(s)	1370
Eco105I	TACGTA	1 cut(s)	173
Eco130I	CCWWGG	2 cut(s)	304, 815
Eco24I	GRGCYC	1 cut(s)	1372
Eco47I	GGWCC	6 cut(s)	1184, 1229, 1262, 1271, 1385, 1421
Eco53kI	GAGCTC	1 cut(s)	1370
Eco57I	CTGAAG	2 cut(s)	251, 856
Eco88I	CYCGRG	2 cut(s)	32, 1468
EcoICRI	GAGCTC	1 cut(s)	1370
EcoNI	CCTNNNNNAGG	2 cut(s)	850, 1356
EcoO109I	RGGNCCY	2 cut(s)	621, 1394
EcoRII	CCWGG	5 cut(s)	283, 394, 724, 796, 1267
EcoT14I	CCWWGG	2 cut(s)	304, 815
EcoT38I	GRGCYC	1 cut(s)	1372
ErhI	CCWWGG	2 cut(s)	304, 815
FaqI	GGGAC	1 cut(s)	305
FauI	CCCGC	1 cut(s)	1298
FauNDI	CATATG	1 cut(s)	1018
FbaI	TGATCA	1 cut(s)	574
FokI	GGATG	4 cut(s)	311, 965, 1092, 1179
FriOI	GRGCYC	1 cut(s)	1372
FspBI	CTAG	4 cut(s)	383, 1290, 1389, 1398
GlaI	GCGC	1 cut(s)	1067
GsaI	CCCAGC	2 cut(s)	662, 1236
GsuI	CTGGAG	1 cut(s)	926
HaeII	RGCGCY	1 cut(s)	1069
HaeIII	GGCC	7 cut(s)	622, 657, 796, 844, 1177, 1396, 1407
HapII	CCGG	1 cut(s)	450
HhaI	GCGC	1 cut(s)	1068
Hin6I	GCGC	1 cut(s)	1066
HinP1I	GCGC	1 cut(s)	1066
HindIII	AAGCTT	1 cut(s)	236
HinfI	GANTC	5 cut(s)	226, 472, 1006, 1022, 1483
HpaII	CCGG	1 cut(s)	450
HphI	GGTGA	5 cut(s)	110, 158, 273, 476, 768
Hpy166II	GTNNAC	3 cut(s)	121, 1151, 1265
Hpy188I	TCNGA	3 cut(s)	231, 875, 1488
Hpy188III	TCNNGA	9 cut(s)	32, 245, 290, 368, 572, 896, 959, 1039, 1470
Hpy8I	GTNNAC	3 cut(s)	121, 1151, 1265
HpyAV	CCTTC	2 cut(s)	95, 185
HpyCH4III	ACNGT	6 cut(s)	113, 412, 488, 561, 1155, 1515
HpyCH4IV	ACGT	2 cut(s)	172, 1363
HpyCH4V	TGCA	8 cut(s)	557, 687, 706, 710, 839, 1102, 1117, 1181
HpyF10VI	GCNNNNNNNGC	9 cut(s)	72, 451, 841, 953, 962, 1060, 1063, 1111, 1464
HpyF3I	CTNAG	1 cut(s)	401
HpySE526I	ACGT	2 cut(s)	172, 1363
HspAI	GCGC	1 cut(s)	1066
Ksp22I	TGATCA	1 cut(s)	574
Kzo9I	GATC	9 cut(s)	56, 364, 388, 574, 773, 883, 925, 982, 1343
LmnI	GCTCC	3 cut(s)	1171, 1367, 1375
Lsp1109I	GCAGC	8 cut(s)	34, 75, 1075, 1094, 1101, 1114, 1129, 1150
LweI	GCATC	6 cut(s)	20, 348, 801, 943, 952, 1114
MabI	ACCWGGT	2 cut(s)	283, 1267
MaeI	CTAG	4 cut(s)	383, 1290, 1389, 1398
MaeII	ACGT	2 cut(s)	172, 1363
MaeIII	GTNAC	4 cut(s)	274, 428, 995, 1331
MalI	GATC	9 cut(s)	58, 366, 390, 576, 775, 885, 927, 984, 1345
MbiI	CCGCTC	1 cut(s)	1305
MboI	GATC	9 cut(s)	56, 364, 388, 574, 773, 883, 925, 982, 1343
MboII	GAAGA	4 cut(s)	244, 862, 862, 1485
MfeI	CAATTG	3 cut(s)	440, 663, 746
MflI	RGATCY	1 cut(s)	56
MhlI	GDGCHC	1 cut(s)	1372
MlyI	GAGTC	1 cut(s)	1015
MmeI	TCCRAC	1 cut(s)	1399
MroXI	GAANNNNTTC	1 cut(s)	195
MseI	TTAA	2 cut(s)	423, 533
MslI	CAYNNNNRTG	2 cut(s)	249, 344
MspA1I	CMGCKG	2 cut(s)	262, 1114
MspI	CCGG	1 cut(s)	450
MspR9I	CCNGG	6 cut(s)	285, 396, 451, 726, 798, 1269
MunI	CAATTG	3 cut(s)	440, 663, 746
MvaI	CCWGG	5 cut(s)	285, 396, 726, 798, 1269
MvnI	CGCG	1 cut(s)	1258
MwoI	GCNNNNNNNGC	9 cut(s)	72, 451, 841, 953, 962, 1060, 1063, 1111, 1464
NciI	CCSGG	1 cut(s)	451
NdeI	CATATG	1 cut(s)	1018
NdeII	GATC	9 cut(s)	56, 364, 388, 574, 773, 883, 925, 982, 1343
NlaIV	GGNNCC	3 cut(s)	39, 623, 1231
NmeAIII	GCCGAG	2 cut(s)	991, 1349
NmuCI	GTSAC	2 cut(s)	274, 995
NspI	RCATGY	3 cut(s)	673, 710, 893
PaeI	GCATGC	2 cut(s)	710, 893
PaeR7I	CTCGAG	1 cut(s)	32
PagI	TCATGA	3 cut(s)	244, 367, 571
PciI	ACATGT	1 cut(s)	669
PdmI	GAANNNNTTC	1 cut(s)	195
PfeI	GAWTC	4 cut(s)	226, 472, 1022, 1483
Pfl23II	CGTACG	1 cut(s)	1410
PfoI	TCCNGGA	1 cut(s)	394
PleI	GAGTC	1 cut(s)	1014
PpsI	GAGTC	1 cut(s)	1014
Ppu21I	YACGTR	1 cut(s)	173
PscI	ACATGT	1 cut(s)	669
PsiI	TTATAA	1 cut(s)	1314
Psp124BI	GAGCTC	1 cut(s)	1372
Psp6I	CCWGG	5 cut(s)	283, 394, 724, 796, 1267
PspFI	CCCAGC	2 cut(s)	658, 1232
PspGI	CCWGG	5 cut(s)	283, 394, 724, 796, 1267
PspLI	CGTACG	1 cut(s)	1410
PspN4I	GGNNCC	3 cut(s)	39, 623, 1231
PstI	CTGCAG	3 cut(s)	1104, 1119, 1183
PstNI	CAGNNNCTG	1 cut(s)	1099
PsuI	RGATCY	1 cut(s)	56
PvuII	CAGCTG	1 cut(s)	262
RsaI	GTAC	4 cut(s)	359, 971, 1221, 1412
RsaNI	GTAC	4 cut(s)	358, 970, 1220, 1411
RseI	CAYNNNNRTG	2 cut(s)	249, 344
SacI	GAGCTC	1 cut(s)	1372
SaqAI	TTAA	2 cut(s)	423, 533
Sau3AI	GATC	9 cut(s)	56, 364, 388, 574, 773, 883, 925, 982, 1343
SbfI	CCTGCAGG	1 cut(s)	1183
ScaI	AGTACT	1 cut(s)	971
SchI	GAGTC	1 cut(s)	1015
ScrFI	CCNGG	6 cut(s)	285, 396, 451, 726, 798, 1269
SdaI	CCTGCAGG	1 cut(s)	1183
SduI	GDGCHC	1 cut(s)	1372
SexAI	ACCWGGT	2 cut(s)	283, 1267
SfaNI	GCATC	6 cut(s)	20, 348, 801, 943, 952, 1114
SfcI	CTRYAG	3 cut(s)	1100, 1115, 1179
Sfr274I	CTCGAG	1 cut(s)	32
SinI	GGWCC	6 cut(s)	1184, 1229, 1262, 1271, 1385, 1421
SlaI	CTCGAG	1 cut(s)	32
SmiMI	CAYNNNNRTG	2 cut(s)	249, 344
SmlI	CTYRAG	1 cut(s)	32
SmoI	CTYRAG	1 cut(s)	32
SnaBI	TACGTA	1 cut(s)	173
SphI	GCATGC	2 cut(s)	710, 893
Sse8387I	CCTGCAGG	1 cut(s)	1183
SsiI	CCGC	6 cut(s)	1054, 1112, 1256, 1279, 1293, 1305
SspMI	CTAG	4 cut(s)	383, 1290, 1389, 1398
SstI	GAGCTC	1 cut(s)	1372
StyD4I	CCNGG	6 cut(s)	283, 394, 449, 724, 796, 1267
StyI	CCWWGG	2 cut(s)	304, 815
TaaI	ACNGT	6 cut(s)	113, 412, 488, 561, 1155, 1515
TaiI	ACGT	2 cut(s)	175, 1366
TaqI	TCGA	3 cut(s)	33, 87, 516
TatI	WGTACW	2 cut(s)	357, 969
TauI	GCSGC	2 cut(s)	1057, 1258
TfiI	GAWTC	4 cut(s)	226, 472, 1022, 1483
Tru1I	TTAA	2 cut(s)	423, 533
Tru9I	TTAA	2 cut(s)	423, 533
TscAI	CASTG	1 cut(s)	1345
TseFI	GTSAC	2 cut(s)	274, 995
TseI	GCWGC	8 cut(s)	22, 63, 1063, 1082, 1102, 1114, 1117, 1163
Tsp45I	GTSAC	2 cut(s)	274, 995
TspDTI	ATGAA	6 cut(s)	198, 230, 261, 449, 798, 1035
TspGWI	ACGGA	2 cut(s)	975, 1518
TspRI	CASTG	1 cut(s)	1345
VpaK11BI	GGWCC	6 cut(s)	1184, 1229, 1262, 1271, 1385, 1421
XagI	CCTNNNNNAGG	2 cut(s)	850, 1356
XapI	RAATTY	1 cut(s)	529
XceI	RCATGY	3 cut(s)	673, 710, 893
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1244
XhoI	CTCGAG	1 cut(s)	32
XmnI	GAANNNNTTC	1 cut(s)	195
XspI	CTAG	4 cut(s)	383, 1290, 1389, 1398
ZrmI	AGTACT	1 cut(s)	971

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.