Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002129

Physical Location & Seq

Genomic Coordinates Forward (+)

4191735 .. 4201783

Gene Structure 10049 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1037600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGGCGATAGCGGCCCCTGGGCAGCTCAACCTCAACGAGGTCCCTTCATGGGGTTCCAGAAGCGTCGACTGCTTCGAGAAACTGGAACAAATTGGCGAGGGAACCTATGGCCAAGTTTACATGGCTAGAGAAATAAAGACGGGAGAAATTGTCGCTTTGAAGAAAATTCGGATGGACAATGAGAGAGAGGGGTTCCCCATAACTGCCATACGGGAAATTAAGATTCTTAAGAAGCTTCACCATGATAATGTCATCAATTTGAAAGAGATCGTGACTTCCCCAGGTCCAGAGAAGGACGAGCAAGGTAGACCAGATGGTAATAAGTATAAAGGTGGCATCTATATGGTCTTTGAATACATGGACCATGATTTGACAGGTCTAGCTGATCGACCTGGGATGAGATTTTCAGTCGCCCAGATTAAGTGCTACATGAAACAACTTTTGACCGGGCTTCACTATTGTCATGTGAATCAAGTACTTCACCGAGATATTAAAGGCTCTAATCTACTTATAGACAATGAAGGCAATCTGAAGCTTGCAGATTTTGGGCTTGCCCGTTCATTTTCAAATGATCACAATGCCAATCTTACAAATCGTGTTATTACTCTTTGGTACAGACCTCCAGAGTTGCTTCTTGGAGCTACAAAGTATGGTCCAGCTGTAGATATGTGGTCCGTTGGGTGTATCTTTGCTGAGCTTCTACATGGCAAGCCAATTTTTCCCGGGAAAGATGAGCCAGAGCAATTAAATAAGATTTTTGAGCTGTGTGGAGCTCCAGATGAGGTCAACTGGCCTGGAGTTTCGAAGATTCCATGGTATAACAACTTCAAGCCCACAAGGCCACTGAAGAAACGTCTCAGGGATGTTTTCAGACATTTTGACCGTCATGCTTTGGAGTTGTTGGAGAAAATGCTGACACTTGATCCTTCTCAGAGAATATCAGCTAAGGATGCTCTTGATGCCGAGTATTTCTGGACTGATCCATTACCTTGTGATCCTAAGAGTCTACCAAAATATGAATCATCACACGAGTTCCAGACAAAGAAAAAGCGCCAGCAGCAACGGCAACATGAAGAGAATGCAAAACGTCAGAAACTGCAGCACCCTCAGCAGCATACCCGCCTCCCACCGATTCAGCAATCTGGCCAAGCACATTCCCAAATGCGGCCTGGACCTAACCAACCTATCCATGGTTCTCAGCCCCCGGTTGCTACAGGACCTAGCCACCATTATGGTAAGCCACGCGGGCCCTCCGGAGGGCCAAGCAGATACCCACCTGGTGGGAACCCTGGCGGTGGATACAACCATCCAAGTCGGGGTGCTCAAGGTGGCGGAGGTGGGTACAGCAGTGGCCCATATCCTGCACAAGGGCGGGGAGCACCATATGGTTCTAGTGGCATACCTGGTGCAGGTCCTCGGGGTGGAGGCAGTGGTTATGGAGTTGGTGGCCCAAACTATCCTCAGAATGGTCCCTATGGTGGCTCTGCAGCAGGTCGGGGCTCAAACATGATGGGTGGAAACCGCAATCAACAGTATGGTTGGCAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0065007 GO:0071704 GO:0097472 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

517

Amino Acids

57.03

Weight (kDa)

9.29

Isoelectric Point (pI)

37.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	25 - 324	6.6e-74	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	27 - 252	2.2e-29	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002129)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10270 AT5G64960 AT5G64960
fragaria_vesca	FvH4_1g20740 FvH4_1g20740 FvH4_1g20740 FvH4_1g20740 FvH4_4g11370 FvH4_4g11370 FvH4_4g11370
malus_domestica	MD04G1037600.v1.1 MD15G1318300.v1.1
prunus_persica	Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.6G187600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0113361 RchiOBHm_Chr2g0113381 RchiOBHm_Chr4g0407881
rosa_laevigata	RLG00000008692 RLG00000018058
rosa_multiflora	Rmu_sc0002073.1_g000029 Rmu_sc0007621.1_g000001 Rmu_sc0011967.1_g000006 Rmu_sc0029075.1_g000002 Rmu_sc0029867.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129320 Rroxscaffold_2G00129850 Rroxscaffold_5G00352420
rosa_rugosa	Rorug02G0186500 Rorug04G0081800 Rorug04G0081900
rosa_samantha	Rh2AG241100 Rh2BG255300 Rh2CG245400 Rh2DG249100 Rh4AG147000 Rh4BG144500 Rh4CG153800 Rh4DG141000
rosa_wichuraiana	Rw2G018830 Rw4G012070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1400, 1481
AccB7I	CCANNNNNTGG	1 cut(s)	1280
AccI	GTMKAC	3 cut(s)	66, 307, 1006
AccII	CGCG	1 cut(s)	1245
AccIII	TCCGGA	1 cut(s)	1253
AciI	CCGC	8 cut(s)	11, 1120, 1165, 1245, 1293, 1332, 1372, 1522
AclWI	GGATC	3 cut(s)	917, 974, 989
AcoI	YGGCCR	2 cut(s)	109, 1144
AcsI	RAATTY	1 cut(s)	165
AcuI	CTGAAG	2 cut(s)	551, 866
AdeI	CACNNNGTG	1 cut(s)	1280
AfaI	GTAC	3 cut(s)	477, 614, 1343
AflII	CTTAAG	1 cut(s)	227
AgsI	TTSAA	5 cut(s)	160, 262, 353, 567, 829
AjnI	CCWGG	8 cut(s)	16, 280, 391, 793, 1168, 1276, 1288, 1402
Alw21I	GWGCWC	3 cut(s)	775, 1324, 1381
Alw26I	GTCTC	1 cut(s)	860
AlwI	GGATC	3 cut(s)	917, 974, 989
AlwNI	CAGNNNCTG	1 cut(s)	1096
Ama87I	CYCGRG	2 cut(s)	722, 1416
Aor13HI	TCCGGA	1 cut(s)	1253
ApaI	GGGCCC	1 cut(s)	1251
ApeKI	GCWGC	6 cut(s)	22, 1057, 1099, 1111, 1487, 1543
ApoI	RAATTY	1 cut(s)	165
AspLEI	GCGC	1 cut(s)	1053
AsuC2I	CCSGG	4 cut(s)	448, 723, 724, 1205
AsuHPI	GGTGA	2 cut(s)	230, 473
AsuII	TTCGAA	1 cut(s)	803
AvaI	CYCGRG	2 cut(s)	722, 1416
AvaII	GGWCC	9 cut(s)	40, 284, 361, 653, 672, 1172, 1217, 1412, 1469
BaeGI	GKGCMC	1 cut(s)	1251
BaeI	ACNNNNGTAYC	2 cut(s)	1333, 1366
BalI	TGGCCA	2 cut(s)	111, 1146
BanII	GRGCYC	3 cut(s)	775, 1251, 1502
BarI	GAAGNNNNNNTAC	2 cut(s)	809, 841
BauI	CACGAG	1 cut(s)	1028
Bbv12I	GWGCWC	3 cut(s)	775, 1324, 1381
BbvCI	CCTCAGC	1 cut(s)	1107
BbvI	GCAGC	5 cut(s)	34, 1069, 1111, 1123, 1499
BccI	CCATC	4 cut(s)	166, 308, 1314, 1504
BceAI	ACGGC	1 cut(s)	1079
BciT130I	CCWGG	8 cut(s)	18, 282, 393, 795, 1170, 1278, 1290, 1404
BciVI	GTATCC	1 cut(s)	1292
BclI	TGATCA	1 cut(s)	571
BcnI	CCSGG	4 cut(s)	448, 723, 724, 1205
BcoDI	GTCTC	1 cut(s)	860
BfaI	CTAG	4 cut(s)	126, 380, 1221, 1392
BfmI	CTRYAG	4 cut(s)	660, 1097, 1212, 1485
BfoI	RGCGCY	1 cut(s)	1054
BfrI	CTTAAG	1 cut(s)	227
BfuAI	ACCTGC	2 cut(s)	1400, 1481
BfuI	GTATCC	1 cut(s)	1292
BglI	GCCNNNNNGGC	2 cut(s)	838, 1246
BisI	GCNGC	8 cut(s)	12, 23, 1058, 1100, 1112, 1166, 1488, 1544
BlpI	GCTNAGC	1 cut(s)	693
BlsI	GCNGC	8 cut(s)	13, 24, 1059, 1101, 1113, 1167, 1489, 1545
BmcAI	AGTACT	1 cut(s)	477
Bme18I	GGWCC	9 cut(s)	40, 284, 361, 653, 672, 1172, 1217, 1412, 1469
BmeT110I	CYCGRG	2 cut(s)	722, 1416
BmiI	GGNNCC	8 cut(s)	15, 42, 55, 103, 194, 1249, 1286, 1471
BmsI	GCATC	3 cut(s)	345, 940, 949
BpmI	CTGGAG	3 cut(s)	606, 759, 816
Bpu10I	CCTNAGC	2 cut(s)	945, 1107
Bpu1102I	GCTNAGC	1 cut(s)	693
Bpu14I	TTCGAA	1 cut(s)	803
BpuEI	CTTGAG	1 cut(s)	1308
BpuMI	CCSGG	4 cut(s)	448, 723, 724, 1205
BsaWI	WCCGGW	1 cut(s)	1253
BsaXI	ACNNNNNCTCC	2 cut(s)	135, 165
Bse1I	ACTGG	2 cut(s)	87, 794
BseAI	TCCGGA	1 cut(s)	1253
BseBI	CCWGG	8 cut(s)	18, 282, 393, 795, 1170, 1278, 1290, 1404
BseGI	GGATG	5 cut(s)	177, 402, 868, 955, 1306
BseMII	CTCAG	6 cut(s)	684, 871, 944, 1121, 1211, 1475
BseNI	ACTGG	2 cut(s)	87, 794
BseSI	GKGCMC	1 cut(s)	1251
BseXI	GCAGC	5 cut(s)	34, 1069, 1111, 1123, 1499
BsgI	GTGCAG	2 cut(s)	1347, 1428
Bsh1236I	CGCG	1 cut(s)	1245
BsiHKAI	GWGCWC	3 cut(s)	775, 1324, 1381
BsiHKCI	CYCGRG	2 cut(s)	722, 1416
BsiSI	CCGG	4 cut(s)	447, 723, 1205, 1254
BslFI	GGGAC	2 cut(s)	26, 1455
BsmAI	GTCTC	1 cut(s)	860
BsmBI	CGTCTC	1 cut(s)	860
BsmFI	GGGAC	2 cut(s)	26, 1455
BsmI	GAATGC	1 cut(s)	1084
BsoBI	CYCGRG	2 cut(s)	722, 1416
Bsp119I	TTCGAA	1 cut(s)	803
Bsp120I	GGGCCC	1 cut(s)	1247
Bsp1286I	GDGCHC	5 cut(s)	775, 1251, 1324, 1381, 1502
Bsp13I	TCCGGA	1 cut(s)	1253
Bsp143I	GATC	6 cut(s)	267, 385, 571, 922, 979, 994
Bsp1720I	GCTNAGC	1 cut(s)	693
Bsp19I	CCATGG	2 cut(s)	812, 1189
BspACI	CCGC	8 cut(s)	11, 1120, 1165, 1245, 1293, 1332, 1372, 1522
BspCNI	CTCAG	6 cut(s)	685, 870, 943, 1120, 1210, 1474
BspEI	TCCGGA	1 cut(s)	1253
BspFNI	CGCG	1 cut(s)	1245
BspLI	GGNNCC	8 cut(s)	15, 42, 55, 103, 194, 1249, 1286, 1471
BspMAI	CTGCAG	2 cut(s)	1101, 1489
BspMI	ACCTGC	2 cut(s)	1400, 1481
BspPI	GGATC	3 cut(s)	917, 974, 989
BspT104I	TTCGAA	1 cut(s)	803
BspTI	CTTAAG	1 cut(s)	227
BsrI	ACTGG	2 cut(s)	87, 794
BssMI	GATC	6 cut(s)	267, 385, 571, 922, 979, 994
BssSI	CACGAG	1 cut(s)	1028
BssT1I	CCWWGG	2 cut(s)	812, 1189
Bst2BI	CACGAG	1 cut(s)	1028
Bst2UI	CCWGG	8 cut(s)	18, 282, 393, 795, 1170, 1278, 1290, 1404
Bst4CI	ACNGT	2 cut(s)	884, 1533
Bst6I	CTCTTC	1 cut(s)	1068
BstAFI	CTTAAG	1 cut(s)	227
BstBI	TTCGAA	1 cut(s)	803
BstC8I	GCNNGC	5 cut(s)	537, 552, 710, 1055, 1247
BstDEI	CTNAG	8 cut(s)	693, 857, 930, 945, 999, 1107, 1197, 1461
BstDSI	CCRYGG	2 cut(s)	812, 1189
BstENI	CCTNNNNNAGG	3 cut(s)	35, 1365, 1407
BstF5I	GGATG	5 cut(s)	177, 402, 868, 955, 1306
BstFNI	CGCG	1 cut(s)	1245
BstH2I	RGCGCY	1 cut(s)	1054
BstHHI	GCGC	1 cut(s)	1053
BstKTI	GATC	6 cut(s)	270, 388, 574, 925, 982, 997
BstMAI	GTCTC	1 cut(s)	860
BstMBI	GATC	6 cut(s)	267, 385, 571, 922, 979, 994
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 69, 838, 950, 959, 1057, 1063, 1108, 1246
BstNI	CCWGG	8 cut(s)	18, 282, 393, 795, 1170, 1278, 1290, 1404
BstSFI	CTRYAG	4 cut(s)	660, 1097, 1212, 1485
BstSLI	GKGCMC	1 cut(s)	1251
BstUI	CGCG	1 cut(s)	1245
BstV1I	GCAGC	5 cut(s)	34, 1069, 1111, 1123, 1499
BstXI	CCANNNNNNTGG	1 cut(s)	1232
BsuI	GTATCC	1 cut(s)	1292
BtgI	CCRYGG	2 cut(s)	812, 1189
BtsCI	GGATG	5 cut(s)	177, 402, 868, 955, 1306
BtsI	GCAGTG	2 cut(s)	1354, 1435
BtsIMutI	CAGTG	3 cut(s)	842, 1354, 1435
BveI	ACCTGC	2 cut(s)	1400, 1481
Cac8I	GCNNGC	5 cut(s)	537, 552, 710, 1055, 1247
CaiI	CAGNNNCTG	1 cut(s)	1096
CfoI	GCGC	1 cut(s)	1053
Cfr9I	CCCGGG	1 cut(s)	722
CseI	GACGC	1 cut(s)	52
CsiI	ACCWGGT	2 cut(s)	1276, 1402
Csp6I	GTAC	3 cut(s)	476, 613, 1342
CviQI	GTAC	3 cut(s)	476, 613, 1342
DdeI	CTNAG	8 cut(s)	693, 857, 930, 945, 999, 1107, 1197, 1461
DpnI	GATC	6 cut(s)	269, 387, 573, 924, 981, 996
DpnII	GATC	6 cut(s)	267, 385, 571, 922, 979, 994
DraIII	CACNNNGTG	1 cut(s)	1280
EaeI	YGGCCR	2 cut(s)	109, 1144
Eam1104I	CTCTTC	1 cut(s)	1068
EarI	CTCTTC	1 cut(s)	1068
EciI	GGCGGA	1 cut(s)	1347
Ecl136II	GAGCTC	1 cut(s)	773
Eco130I	CCWWGG	2 cut(s)	812, 1189
Eco24I	GRGCYC	3 cut(s)	775, 1251, 1502
Eco47I	GGWCC	9 cut(s)	40, 284, 361, 653, 672, 1172, 1217, 1412, 1469
Eco53kI	GAGCTC	1 cut(s)	773
Eco57I	CTGAAG	2 cut(s)	551, 866
Eco88I	CYCGRG	2 cut(s)	722, 1416
EcoICRI	GAGCTC	1 cut(s)	773
EcoNI	CCTNNNNNAGG	3 cut(s)	35, 1365, 1407
EcoO109I	RGGNCCY	4 cut(s)	40, 1217, 1248, 1412
EcoRII	CCWGG	8 cut(s)	16, 280, 391, 793, 1168, 1276, 1288, 1402
EcoT14I	CCWWGG	2 cut(s)	812, 1189
EcoT38I	GRGCYC	3 cut(s)	775, 1251, 1502
ErhI	CCWWGG	2 cut(s)	812, 1189
Esp3I	CGTCTC	1 cut(s)	860
FaqI	GGGAC	2 cut(s)	26, 1455
FauI	CCCGC	3 cut(s)	1127, 1238, 1365
FauNDI	CATATG	1 cut(s)	1384
FbaI	TGATCA	1 cut(s)	571
FblI	GTMKAC	3 cut(s)	66, 307, 1006
Fnu4HI	GCNGC	8 cut(s)	12, 23, 1058, 1100, 1112, 1166, 1488, 1544
FokI	GGATG	5 cut(s)	184, 409, 875, 962, 1293
FriOI	GRGCYC	3 cut(s)	775, 1251, 1502
Fsp4HI	GCNGC	8 cut(s)	12, 23, 1058, 1100, 1112, 1166, 1488, 1544
FspBI	CTAG	4 cut(s)	126, 380, 1221, 1392
GlaI	GCGC	1 cut(s)	1052
GluI	GCNGC	8 cut(s)	12, 23, 1058, 1100, 1112, 1166, 1488, 1544
GsuI	CTGGAG	3 cut(s)	606, 759, 816
HaeII	RGCGCY	1 cut(s)	1054
HapII	CCGG	4 cut(s)	447, 723, 1205, 1254
HgaI	GACGC	1 cut(s)	52
HhaI	GCGC	1 cut(s)	1053
Hin6I	GCGC	1 cut(s)	1051
HinP1I	GCGC	1 cut(s)	1051
HincII	GTYRAC	2 cut(s)	67, 787
HindII	GTYRAC	2 cut(s)	67, 787
HindIII	AAGCTT	2 cut(s)	233, 533
HinfI	GANTC	6 cut(s)	223, 469, 808, 1003, 1019, 1132
HpaII	CCGG	4 cut(s)	447, 723, 1205, 1254
HphI	GGTGA	2 cut(s)	230, 473
Hpy166II	GTNNAC	5 cut(s)	67, 118, 308, 787, 1007
Hpy188I	TCNGA	6 cut(s)	171, 531, 872, 933, 1092, 1464
Hpy8I	GTNNAC	5 cut(s)	67, 118, 308, 787, 1007
Hpy99I	CGWCG	1 cut(s)	68
HpyAV	CCTTC	4 cut(s)	54, 286, 515, 936
HpyCH4III	ACNGT	2 cut(s)	884, 1533
HpyCH4IV	ACGT	2 cut(s)	853, 1087
HpyCH4V	TGCA	6 cut(s)	539, 1082, 1099, 1364, 1409, 1487
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 69, 838, 950, 959, 1057, 1063, 1108, 1246
HpyF3I	CTNAG	8 cut(s)	693, 857, 930, 945, 999, 1107, 1197, 1461
HpySE526I	ACGT	2 cut(s)	853, 1087
HspAI	GCGC	1 cut(s)	1051
Kpn2I	TCCGGA	1 cut(s)	1253
Ksp22I	TGATCA	1 cut(s)	571
Kzo9I	GATC	6 cut(s)	267, 385, 571, 922, 979, 994
LmnI	GCTCC	4 cut(s)	638, 770, 778, 1376
Lsp1109I	GCAGC	5 cut(s)	34, 1069, 1111, 1123, 1499
LweI	GCATC	3 cut(s)	345, 940, 949
MabI	ACCWGGT	2 cut(s)	1276, 1402
MaeI	CTAG	4 cut(s)	126, 380, 1221, 1392
MaeII	ACGT	2 cut(s)	853, 1087
MaeIII	GTNAC	1 cut(s)	271
MalI	GATC	6 cut(s)	269, 387, 573, 924, 981, 996
MboI	GATC	6 cut(s)	267, 385, 571, 922, 979, 994
MboII	GAAGA	4 cut(s)	172, 817, 859, 1085
MhlI	GDGCHC	5 cut(s)	775, 1251, 1324, 1381, 1502
MlsI	TGGCCA	2 cut(s)	111, 1146
MluCI	AATT	7 cut(s)	90, 147, 165, 216, 256, 714, 743
MluNI	TGGCCA	2 cut(s)	111, 1146
MlyI	GAGTC	1 cut(s)	1012
MmeI	TCCRAC	1 cut(s)	882
Mox20I	TGGCCA	2 cut(s)	111, 1146
MroI	TCCGGA	1 cut(s)	1253
MscI	TGGCCA	2 cut(s)	111, 1146
MseI	TTAA	5 cut(s)	219, 228, 420, 492, 746
MslI	CAYNNNNRTG	3 cut(s)	246, 341, 1230
Msp20I	TGGCCA	2 cut(s)	111, 1146
MspA1I	CMGCKG	1 cut(s)	659
MspCI	CTTAAG	1 cut(s)	227
MspI	CCGG	4 cut(s)	447, 723, 1205, 1254
Mva1269I	GAATGC	1 cut(s)	1084
MvaI	CCWGG	8 cut(s)	18, 282, 393, 795, 1170, 1278, 1290, 1404
MvnI	CGCG	1 cut(s)	1245
MwoI	GCNNNNNNNGC	9 cut(s)	11, 69, 838, 950, 959, 1057, 1063, 1108, 1246
NciI	CCSGG	4 cut(s)	448, 723, 724, 1205
NcoI	CCATGG	2 cut(s)	812, 1189
NdeI	CATATG	1 cut(s)	1384
NdeII	GATC	6 cut(s)	267, 385, 571, 922, 979, 994
NlaIV	GGNNCC	8 cut(s)	15, 42, 55, 103, 194, 1249, 1286, 1471
NmeAIII	GCCGAG	1 cut(s)	988
NmuCI	GTSAC	1 cut(s)	271
NspV	TTCGAA	1 cut(s)	803
PasI	CCCWGGG	1 cut(s)	17
PcsI	WCGNNNNNNNCGW	1 cut(s)	72
PctI	GAATGC	1 cut(s)	1084
PfeI	GAWTC	5 cut(s)	223, 469, 808, 1019, 1132
PflMI	CCANNNNNTGG	1 cut(s)	1280
PkrI	GCNGC	8 cut(s)	13, 24, 1059, 1101, 1113, 1167, 1489, 1545
PleI	GAGTC	1 cut(s)	1011
PpsI	GAGTC	1 cut(s)	1011
PpuMI	RGGWCCY	3 cut(s)	40, 1217, 1412
Psp124BI	GAGCTC	1 cut(s)	775
Psp5II	RGGWCCY	3 cut(s)	40, 1217, 1412
Psp6I	CCWGG	8 cut(s)	16, 280, 391, 793, 1168, 1276, 1288, 1402
PspGI	CCWGG	8 cut(s)	16, 280, 391, 793, 1168, 1276, 1288, 1402
PspN4I	GGNNCC	8 cut(s)	15, 42, 55, 103, 194, 1249, 1286, 1471
PspOMI	GGGCCC	1 cut(s)	1247
PspPPI	RGGWCCY	3 cut(s)	40, 1217, 1412
PstI	CTGCAG	2 cut(s)	1101, 1489
PstNI	CAGNNNCTG	1 cut(s)	1096
PvuII	CAGCTG	1 cut(s)	659
RsaI	GTAC	3 cut(s)	477, 614, 1343
RsaNI	GTAC	3 cut(s)	476, 613, 1342
RseI	CAYNNNNRTG	3 cut(s)	246, 341, 1230
SacI	GAGCTC	1 cut(s)	775
SalI	GTCGAC	1 cut(s)	65
SaqAI	TTAA	5 cut(s)	219, 228, 420, 492, 746
SatI	GCNGC	8 cut(s)	12, 23, 1058, 1100, 1112, 1166, 1488, 1544
Sau3AI	GATC	6 cut(s)	267, 385, 571, 922, 979, 994
ScaI	AGTACT	1 cut(s)	477
SchI	GAGTC	1 cut(s)	1012
SduI	GDGCHC	5 cut(s)	775, 1251, 1324, 1381, 1502
SexAI	ACCWGGT	2 cut(s)	1276, 1402
SfaNI	GCATC	3 cut(s)	345, 940, 949
SfcI	CTRYAG	4 cut(s)	660, 1097, 1212, 1485
SfuI	TTCGAA	1 cut(s)	803
SinI	GGWCC	9 cut(s)	40, 284, 361, 653, 672, 1172, 1217, 1412, 1469
SmaI	CCCGGG	1 cut(s)	724
SmiMI	CAYNNNNRTG	3 cut(s)	246, 341, 1230
SmlI	CTYRAG	2 cut(s)	227, 1323
SmoI	CTYRAG	2 cut(s)	227, 1323
Sse9I	AATT	7 cut(s)	90, 147, 165, 216, 256, 714, 743
SsiI	CCGC	8 cut(s)	11, 1120, 1165, 1245, 1293, 1332, 1372, 1522
SspMI	CTAG	4 cut(s)	126, 380, 1221, 1392
SstI	GAGCTC	1 cut(s)	775
StyI	CCWWGG	2 cut(s)	812, 1189
TaaI	ACNGT	2 cut(s)	884, 1533
TaiI	ACGT	2 cut(s)	856, 1090
TaqI	TCGA	4 cut(s)	66, 75, 388, 803
TasI	AATT	7 cut(s)	90, 147, 165, 216, 256, 714, 743
TatI	WGTACW	1 cut(s)	475
TauI	GCSGC	2 cut(s)	14, 1168
TfiI	GAWTC	5 cut(s)	223, 469, 808, 1019, 1132
Tru1I	TTAA	5 cut(s)	219, 228, 420, 492, 746
Tru9I	TTAA	5 cut(s)	219, 228, 420, 492, 746
TscAI	CASTG	3 cut(s)	849, 1354, 1435
TseFI	GTSAC	1 cut(s)	271
TseI	GCWGC	6 cut(s)	22, 1057, 1099, 1111, 1487, 1543
Tsp45I	GTSAC	1 cut(s)	271
TspDTI	ATGAA	6 cut(s)	36, 446, 534, 549, 1032, 1086
TspGWI	ACGGA	1 cut(s)	664
TspMI	CCCGGG	1 cut(s)	722
TspRI	CASTG	3 cut(s)	849, 1354, 1435
Van91I	CCANNNNNTGG	1 cut(s)	1280
Vha464I	CTTAAG	1 cut(s)	227
VpaK11BI	GGWCC	9 cut(s)	40, 284, 361, 653, 672, 1172, 1217, 1412, 1469
XagI	CCTNNNNNAGG	3 cut(s)	35, 1365, 1407
XapI	RAATTY	1 cut(s)	165
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1166, 1187
XmaI	CCCGGG	1 cut(s)	722
XmiI	GTMKAC	3 cut(s)	66, 307, 1006
XspI	CTAG	4 cut(s)	126, 380, 1221, 1392
ZrmI	AGTACT	1 cut(s)	477

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.