Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0029867.1

Orthogroup ID

OG0002129

Physical Location & Seq

Genomic Coordinates Reverse (-)

1 .. 3062

Gene Structure 3062 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0029867.1_g000001.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1052 bp

atgaagcagttgttaactgggcttcattactgtcatgttaatcaagtacttcatcgcgacatgaaaggttctaatcttttgttagataatgagggaaaattgaagctagcagactttgggctcgcaaaatcctttgctactaaccatgacgggcagcttacaaatcgtgttattaccttgtggtataggcccccagagttgctgcttggagctacaaagtatggaccagctgtcgacatgtggtctgttggttgcatctttgctgaacttctgaatggttcgccaattttgcaaggaagaactgaacctgaccaactgaacaagatatttgagctttgtggatcgcctgatgagaacaactggcctggtgtttcaaaatattatttttataacaatttcaagccaccaaggcccctgaagcgacgagtcagggaacgtttcagacgttttgacagccatgctcttgatttactggaccacttgttaactcttgatccaaatcagagatggtctgcaaagcaagcactggatgcagagtatttctgggttgatcctttaccttgtgatccgaagagcctgcccaagtatgaatcatcgcatgagtttgaaacaaagaaaaggaagcaggaacagcgacaacttgaagaacaaaacaagagaccaagattacaacatggtcgcctgcctccctatcaacatacagagcctcatcaaatgaaagatacacagccatcattttctgctggacctagtcaccacccctatggaaagcatcctgctcctcctggaggtccaagcatgtaccctcctaggaatcagagtggggcttacaacccaagccgtggaggtcatgttggaggatacagtggtgaaccatatccacctcaaggacgggctccacaatataatggcagcagtatgccagcttctggtcctagaggtccccccggtggttacggagttggtcctcctaactattcccaaactagtcagtatggaggtcctgcccctggtagaggtctgaataatcggagtcaacagtatggccggca

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0065007 GO:0071704 GO:0097472 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

350

Amino Acids

39.42

Weight (kDa)

9.43

Isoelectric Point (pI)

49.23

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002129)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10270 AT5G64960 AT5G64960
fragaria_vesca	FvH4_1g20740 FvH4_1g20740 FvH4_1g20740 FvH4_1g20740 FvH4_4g11370 FvH4_4g11370 FvH4_4g11370
malus_domestica	MD04G1037600.v1.1 MD15G1318300.v1.1
prunus_persica	Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.6G187600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0113361 RchiOBHm_Chr2g0113381 RchiOBHm_Chr4g0407881
rosa_laevigata	RLG00000008692 RLG00000018058
rosa_multiflora	Rmu_sc0002073.1_g000029 Rmu_sc0007621.1_g000001 Rmu_sc0011967.1_g000006 Rmu_sc0029075.1_g000002 Rmu_sc0029867.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129320 Rroxscaffold_2G00129850 Rroxscaffold_5G00352420
rosa_rugosa	Rorug02G0186500 Rorug04G0081800 Rorug04G0081900
rosa_samantha	Rh2AG241100 Rh2BG255300 Rh2CG245400 Rh2DG249100 Rh4AG147000 Rh4BG144500 Rh4CG153800 Rh4DG141000
rosa_wichuraiana	Rw2G018830 Rw4G012070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	390
AccB7I	CCANNNNNTGG	2 cut(s)	842, 929
AccI	GTMKAC	1 cut(s)	234
AccII	CGCG	1 cut(s)	57
AclI	AACGTT	1 cut(s)	436
AclWI	GGATC	4 cut(s)	349, 488, 545, 560
AcoI	YGGCCR	1 cut(s)	1045
AcuI	CTGAAG	1 cut(s)	437
AfaI	GTAC	2 cut(s)	48, 803
AfiI	CCNNNNNNNGG	7 cut(s)	788, 842, 887, 929, 950, 1010, 1016
AflIII	ACRYGT	1 cut(s)	237
AgsI	TTSAA	5 cut(s)	103, 375, 400, 608, 644
AhdI	GACNNNNNGTC	2 cut(s)	230, 241
AhlI	ACTAGT	1 cut(s)	986
AjnI	CCWGG	3 cut(s)	364, 784, 1009
AluBI	AGCT	6 cut(s)	106, 157, 212, 230, 334, 926
AluI	AGCT	6 cut(s)	106, 157, 212, 230, 334, 926
Alw26I	GTCTC	1 cut(s)	652
AlwI	GGATC	4 cut(s)	349, 488, 545, 560
AlwNI	CAGNNNCTG	1 cut(s)	929
AoxI	GGCC	4 cut(s)	188, 362, 410, 1045
ApeKI	GCWGC	3 cut(s)	154, 202, 912
AspA2I	CCTAGG	1 cut(s)	809
AsuC2I	CCSGG	1 cut(s)	948
AsuHPI	GGTGA	2 cut(s)	746, 881
AsuNHI	GCTAGC	1 cut(s)	106
AvaII	GGWCC	8 cut(s)	224, 475, 746, 791, 932, 941, 965, 1001
AvrII	CCTAGG	1 cut(s)	809
BaeI	ACNNNNGTAYC	2 cut(s)	853, 886
BanII	GRGCYC	2 cut(s)	123, 898
BarI	GAAGNNNNNNTAC	2 cut(s)	910, 942
BbvI	GCAGC	3 cut(s)	166, 189, 924
BccI	CCATC	2 cut(s)	501, 739
BceAI	ACGGC	1 cut(s)	825
BcgI	CGANNNNNNTGC	2 cut(s)	559, 593
BciT130I	CCWGG	3 cut(s)	366, 786, 1011
BciVI	GTATCC	1 cut(s)	854
BcnI	CCSGG	1 cut(s)	948
BcoDI	GTCTC	1 cut(s)	652
BcuI	ACTAGT	1 cut(s)	986
BfaI	CTAG	5 cut(s)	107, 750, 810, 936, 987
BfuI	GTATCC	1 cut(s)	854
BglI	GCCNNNNNGGC	1 cut(s)	409
BisI	GCNGC	3 cut(s)	155, 203, 913
BlnI	CCTAGG	1 cut(s)	809
BlsI	GCNGC	3 cut(s)	156, 204, 914
BmcAI	AGTACT	1 cut(s)	48
Bme1390I	CCNGG	4 cut(s)	366, 786, 948, 1011
Bme18I	GGWCC	8 cut(s)	224, 475, 746, 791, 932, 941, 965, 1001
BmeRI	GACNNNNNGTC	2 cut(s)	230, 241
BmiI	GGNNCC	4 cut(s)	191, 413, 897, 943
BmrFI	CCNGG	4 cut(s)	366, 786, 948, 1011
BmrI	ACTGGG	1 cut(s)	27
BmsI	GCATC	3 cut(s)	264, 520, 781
BmtI	GCTAGC	1 cut(s)	110
BmuI	ACTGGG	1 cut(s)	27
BpmI	CTGGAG	1 cut(s)	807
BpuEI	CTTGAG	1 cut(s)	870
BpuMI	CCSGG	1 cut(s)	948
BsaBI	GATNNNNATC	1 cut(s)	498
BsaI	GGTCTC	1 cut(s)	652
BsaJI	CCNNGG	5 cut(s)	407, 809, 841, 946, 1009
BsaXI	ACNNNNNCTCC	2 cut(s)	837, 867
Bsc4I	CCNNNNNNNGG	7 cut(s)	788, 842, 887, 929, 950, 1010, 1016
Bse118I	RCCGGY	1 cut(s)	1047
Bse1I	ACTGG	4 cut(s)	22, 365, 477, 531
Bse8I	GATNNNNATC	1 cut(s)	498
BseBI	CCWGG	3 cut(s)	366, 786, 1011
BseDI	CCNNGG	5 cut(s)	407, 809, 841, 946, 1009
BseGI	GGATG	2 cut(s)	535, 772
BseJI	GATNNNNATC	1 cut(s)	498
BseLI	CCNNNNNNNGG	7 cut(s)	788, 842, 887, 929, 950, 1010, 1016
BseNI	ACTGG	4 cut(s)	22, 365, 477, 531
BseRI	GAGGAG	1 cut(s)	771
BseXI	GCAGC	3 cut(s)	166, 189, 924
Bsh1236I	CGCG	1 cut(s)	57
BshFI	GGCC	4 cut(s)	190, 364, 412, 1047
BsiSI	CCGG	2 cut(s)	948, 1048
BslFI	GGGAC	1 cut(s)	927
BslI	CCNNNNNNNGG	7 cut(s)	788, 842, 887, 929, 950, 1010, 1016
BsmAI	GTCTC	1 cut(s)	652
BsmFI	GGGAC	1 cut(s)	927
BsnI	GGCC	4 cut(s)	190, 364, 412, 1047
Bso31I	GGTCTC	1 cut(s)	652
Bsp1286I	GDGCHC	2 cut(s)	123, 898
Bsp143I	GATC	4 cut(s)	341, 493, 550, 565
Bsp68I	TCGCGA	1 cut(s)	57
BspANI	GGCC	4 cut(s)	190, 364, 412, 1047
BspFNI	CGCG	1 cut(s)	57
BspLI	GGNNCC	4 cut(s)	191, 413, 897, 943
BspOI	GCTAGC	1 cut(s)	110
BspPI	GGATC	4 cut(s)	349, 488, 545, 560
BspQI	GCTCTTC	1 cut(s)	566
BspTNI	GGTCTC	1 cut(s)	652
BsrFI	RCCGGY	1 cut(s)	1047
BsrI	ACTGG	4 cut(s)	22, 365, 477, 531
BssAI	RCCGGY	1 cut(s)	1047
BssECI	CCNNGG	5 cut(s)	407, 809, 841, 946, 1009
BssMI	GATC	4 cut(s)	341, 493, 550, 565
BssT1I	CCWWGG	2 cut(s)	407, 809
Bst2UI	CCWGG	3 cut(s)	366, 786, 1011
Bst4CI	ACNGT	3 cut(s)	32, 866, 1041
Bst6I	CTCTTC	1 cut(s)	566
BstAPI	GCANNNNNTGC	1 cut(s)	530
BstC8I	GCNNGC	7 cut(s)	108, 123, 522, 578, 683, 924, 1049
BstDSI	CCRYGG	1 cut(s)	841
BstENI	CCTNNNNNAGG	2 cut(s)	786, 1014
BstF5I	GGATG	2 cut(s)	535, 772
BstFNI	CGCG	1 cut(s)	57
BstKTI	GATC	4 cut(s)	344, 496, 553, 568
BstMAI	GTCTC	1 cut(s)	652
BstMBI	GATC	4 cut(s)	341, 493, 550, 565
BstMWI	GCNNNNNNNGC	6 cut(s)	289, 409, 418, 521, 530, 631
BstNI	CCWGG	3 cut(s)	366, 786, 1011
BstNSI	RCATGY	2 cut(s)	241, 802
BstSCI	CCNGG	4 cut(s)	364, 784, 946, 1009
BstUI	CGCG	1 cut(s)	57
BstV1I	GCAGC	3 cut(s)	166, 189, 924
BstXI	CCANNNNNNTGG	1 cut(s)	764
BsuI	GTATCC	1 cut(s)	854
BsuRI	GGCC	4 cut(s)	190, 364, 412, 1047
BtgI	CCRYGG	1 cut(s)	841
BtgZI	GCGATG	2 cut(s)	38, 579
BtsCI	GGATG	2 cut(s)	535, 772
BtsIMutI	CAGTG	2 cut(s)	524, 871
BtuMI	TCGCGA	1 cut(s)	57
Cac8I	GCNNGC	7 cut(s)	108, 123, 522, 578, 683, 924, 1049
CaiI	CAGNNNCTG	1 cut(s)	929
Cfr10I	RCCGGY	1 cut(s)	1047
Csp6I	GTAC	2 cut(s)	47, 802
CviAII	CATG	9 cut(s)	35, 61, 146, 238, 458, 599, 674, 799, 851
CviQI	GTAC	2 cut(s)	47, 802
DpnI	GATC	4 cut(s)	343, 495, 552, 567
DpnII	GATC	4 cut(s)	341, 493, 550, 565
DriI	GACNNNNNGTC	2 cut(s)	230, 241
EaeI	YGGCCR	1 cut(s)	1045
Eam1104I	CTCTTC	1 cut(s)	566
Eam1105I	GACNNNNNGTC	2 cut(s)	230, 241
EarI	CTCTTC	1 cut(s)	566
Eco130I	CCWWGG	2 cut(s)	407, 809
Eco24I	GRGCYC	2 cut(s)	123, 898
Eco31I	GGTCTC	1 cut(s)	652
Eco47I	GGWCC	8 cut(s)	224, 475, 746, 791, 932, 941, 965, 1001
Eco57I	CTGAAG	1 cut(s)	437
EcoNI	CCTNNNNNAGG	2 cut(s)	786, 1014
EcoO109I	RGGNCCY	4 cut(s)	189, 411, 941, 1001
EcoRII	CCWGG	3 cut(s)	364, 784, 1009
EcoT14I	CCWWGG	2 cut(s)	407, 809
EcoT38I	GRGCYC	2 cut(s)	123, 898
ErhI	CCWWGG	2 cut(s)	407, 809
FaeI	CATG	9 cut(s)	38, 64, 149, 241, 461, 602, 677, 802, 854
FaqI	GGGAC	1 cut(s)	927
FatI	CATG	9 cut(s)	34, 60, 145, 237, 457, 598, 673, 798, 850
FblI	GTMKAC	1 cut(s)	234
Fnu4HI	GCNGC	3 cut(s)	155, 203, 913
FokI	GGATG	2 cut(s)	542, 759
FriOI	GRGCYC	2 cut(s)	123, 898
Fsp4HI	GCNGC	3 cut(s)	155, 203, 913
FspBI	CTAG	5 cut(s)	107, 750, 810, 936, 987
GluI	GCNGC	3 cut(s)	155, 203, 913
GsuI	CTGGAG	1 cut(s)	807
HaeIII	GGCC	4 cut(s)	190, 364, 412, 1047
HapII	CCGG	2 cut(s)	948, 1048
Hin1II	CATG	9 cut(s)	38, 64, 149, 241, 461, 602, 677, 802, 854
HincII	GTYRAC	4 cut(s)	15, 235, 486, 1037
HindII	GTYRAC	4 cut(s)	15, 235, 486, 1037
HinfI	GANTC	4 cut(s)	426, 590, 814, 1033
HpaI	GTTAAC	2 cut(s)	15, 486
HpaII	CCGG	2 cut(s)	948, 1048
HphI	GGTGA	2 cut(s)	746, 881
Hpy166II	GTNNAC	5 cut(s)	15, 235, 486, 872, 1037
Hpy188I	TCNGA	7 cut(s)	273, 443, 504, 570, 819, 1023, 1032
Hpy188III	TCNNGA	3 cut(s)	56, 464, 491
Hpy8I	GTNNAC	5 cut(s)	15, 235, 486, 872, 1037
Hpy99I	CGWCG	1 cut(s)	426
HpyCH4III	ACNGT	3 cut(s)	32, 866, 1041
HpyCH4IV	ACGT	2 cut(s)	436, 445
HpyCH4V	TGCA	4 cut(s)	255, 292, 515, 533
HpyF10VI	GCNNNNNNNGC	6 cut(s)	289, 409, 418, 521, 530, 631
HpySE526I	ACGT	2 cut(s)	436, 445
Hsp92II	CATG	9 cut(s)	38, 64, 149, 241, 461, 602, 677, 802, 854
KroI	GCCGGC	1 cut(s)	1047
KroNI	GCCGGC	1 cut(s)	1049
KspAI	GTTAAC	2 cut(s)	15, 486
Kzo9I	GATC	4 cut(s)	341, 493, 550, 565
LguI	GCTCTTC	1 cut(s)	566
LmnI	GCTCC	3 cut(s)	209, 784, 901
Lsp1109I	GCAGC	3 cut(s)	166, 189, 924
LweI	GCATC	3 cut(s)	264, 520, 781
MaeI	CTAG	5 cut(s)	107, 750, 810, 936, 987
MaeII	ACGT	2 cut(s)	436, 445
MaeIII	GTNAC	2 cut(s)	752, 953
MalI	GATC	4 cut(s)	343, 495, 552, 567
MboI	GATC	4 cut(s)	341, 493, 550, 565
MboII	GAAGA	3 cut(s)	309, 583, 656
MhlI	GDGCHC	2 cut(s)	123, 898
MluCI	AATT	3 cut(s)	98, 285, 394
MlyI	GAGTC	2 cut(s)	435, 1042
MmeI	TCCRAC	1 cut(s)	835
MroNI	GCCGGC	1 cut(s)	1047
MseI	TTAA	3 cut(s)	14, 39, 485
MslI	CAYNNNNRTG	1 cut(s)	762
MspA1I	CMGCKG	1 cut(s)	230
MspI	CCGG	2 cut(s)	948, 1048
MspR9I	CCNGG	4 cut(s)	366, 786, 948, 1011
MvaI	CCWGG	3 cut(s)	366, 786, 1011
MvnI	CGCG	1 cut(s)	57
MwoI	GCNNNNNNNGC	6 cut(s)	289, 409, 418, 521, 530, 631
NaeI	GCCGGC	1 cut(s)	1049
NciI	CCSGG	1 cut(s)	948
NdeII	GATC	4 cut(s)	341, 493, 550, 565
NgoMIV	GCCGGC	1 cut(s)	1047
NheI	GCTAGC	1 cut(s)	106
NlaIII	CATG	9 cut(s)	38, 64, 149, 241, 461, 602, 677, 802, 854
NlaIV	GGNNCC	4 cut(s)	191, 413, 897, 943
NmuCI	GTSAC	1 cut(s)	752
NruI	TCGCGA	1 cut(s)	57
NspI	RCATGY	2 cut(s)	241, 802
PciI	ACATGT	1 cut(s)	237
PciSI	GCTCTTC	1 cut(s)	566
PcsI	WCGNNNNNNNCGW	1 cut(s)	442
PdiI	GCCGGC	1 cut(s)	1049
PfeI	GAWTC	2 cut(s)	590, 814
PflFI	GACNNNGTC	1 cut(s)	750
PflMI	CCANNNNNTGG	2 cut(s)	842, 929
PfoI	TCCNGGA	1 cut(s)	784
PkrI	GCNGC	3 cut(s)	156, 204, 914
PleI	GAGTC	2 cut(s)	434, 1041
PpsI	GAGTC	2 cut(s)	434, 1041
PpuMI	RGGWCCY	2 cut(s)	941, 1001
PscI	ACATGT	1 cut(s)	237
PsiI	TTATAA	1 cut(s)	390
Psp1406I	AACGTT	1 cut(s)	436
Psp5II	RGGWCCY	2 cut(s)	941, 1001
Psp6I	CCWGG	3 cut(s)	364, 784, 1009
PspGI	CCWGG	3 cut(s)	364, 784, 1009
PspN4I	GGNNCC	4 cut(s)	191, 413, 897, 943
PspPPI	RGGWCCY	2 cut(s)	941, 1001
PstNI	CAGNNNCTG	1 cut(s)	929
PsyI	GACNNNGTC	1 cut(s)	750
PvuII	CAGCTG	1 cut(s)	230
RruI	TCGCGA	1 cut(s)	57
RsaI	GTAC	2 cut(s)	48, 803
RsaNI	GTAC	2 cut(s)	47, 802
RseI	CAYNNNNRTG	1 cut(s)	762
SalI	GTCGAC	1 cut(s)	233
SapI	GCTCTTC	1 cut(s)	566
SaqAI	TTAA	3 cut(s)	14, 39, 485
SatI	GCNGC	3 cut(s)	155, 203, 913
Sau3AI	GATC	4 cut(s)	341, 493, 550, 565
ScaI	AGTACT	1 cut(s)	48
SchI	GAGTC	2 cut(s)	435, 1042
ScrFI	CCNGG	4 cut(s)	366, 786, 948, 1011
SduI	GDGCHC	2 cut(s)	123, 898
SfaNI	GCATC	3 cut(s)	264, 520, 781
SinI	GGWCC	8 cut(s)	224, 475, 746, 791, 932, 941, 965, 1001
SmiMI	CAYNNNNRTG	1 cut(s)	762
SmlI	CTYRAG	1 cut(s)	885
SmoI	CTYRAG	1 cut(s)	885
SpeI	ACTAGT	1 cut(s)	986
Sse9I	AATT	3 cut(s)	98, 285, 394
SspI	AATATT	1 cut(s)	380
SspMI	CTAG	5 cut(s)	107, 750, 810, 936, 987
StyD4I	CCNGG	4 cut(s)	364, 784, 946, 1009
StyI	CCWWGG	2 cut(s)	407, 809
TaaI	ACNGT	3 cut(s)	32, 866, 1041
TaiI	ACGT	2 cut(s)	439, 448
TaqI	TCGA	1 cut(s)	234
TasI	AATT	3 cut(s)	98, 285, 394
TatI	WGTACW	1 cut(s)	46
TfiI	GAWTC	2 cut(s)	590, 814
Tru1I	TTAA	3 cut(s)	14, 39, 485
Tru9I	TTAA	3 cut(s)	14, 39, 485
TscAI	CASTG	2 cut(s)	531, 871
TseFI	GTSAC	1 cut(s)	752
TseI	GCWGC	3 cut(s)	154, 202, 912
Tsp45I	GTSAC	1 cut(s)	752
TspDTI	ATGAA	6 cut(s)	14, 17, 41, 77, 603, 731
TspGWI	ACGGA	1 cut(s)	972
TspRI	CASTG	2 cut(s)	531, 871
Tth111I	GACNNNGTC	1 cut(s)	750
Van91I	CCANNNNNTGG	2 cut(s)	842, 929
VpaK11BI	GGWCC	8 cut(s)	224, 475, 746, 791, 932, 941, 965, 1001
XagI	CCTNNNNNAGG	2 cut(s)	786, 1014
XceI	RCATGY	2 cut(s)	241, 802
XcmI	CCANNNNNNNNNTGG	1 cut(s)	504
XmaJI	CCTAGG	1 cut(s)	809
XmiI	GTMKAC	1 cut(s)	234
XspI	CTAG	5 cut(s)	107, 750, 810, 936, 987
ZrmI	AGTACT	1 cut(s)	48

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.