Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002129

Physical Location & Seq

Genomic Coordinates Reverse (-)

32851375 .. 32856297

Gene Structure 4923 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1318300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGGCTGCAGCAGCACCTGGGCAGATAAACACCGAACCGTTAATTTCGTTGGGTTCTCGAAGCGTCGACTGCTTTAAGAAACTCGAGCACATTGGCGAAGGCACTTATGGTCAAGTGTTCACGGCCAGAGAAACCAGAACTGGGGAAATTGTTGCGTTGAAAAAAATACGCATGGATAATGAAAAAGAAGGGTTCCCTATTACAGCAATCCGGGAAATTAAAATTCTGAAGAAGCTTCATCATGAAAATGTTGTTAAGCTGAAGGAGATTGTAACTTCTGCAGGTCCCGAAAGGGATGAGCTGAGGAATCAGGGTCGTGAAAGGGATGAGCAAGAGAATCAAGGTCCTGAAAGGGATAAGCAAGAGAATCAAGATACTAGTAAGTCCAAGGGTGGCATCTATATGGTTTTTGAGTACATGGATCATGATCTGACAGGCCTTACTGTTCGTTCTGGACAGACATTTACTGTTCCCCAAGTAAAGTGTTATATGAAGCAGTTATTGACTGGGCTCCATTACTGTCATGTTAATAAAGTACTTCACCGTGACATCAAAGGTTCTAATCTTTTGATAGATAATGAGGGGAAGTTGAAGCTAGCAGACTTTGGGCTTGCAAAGTATTATTGTAGTAACCACGAGGGAAATCTTACAAATCGTGTCATTACTTTGTGGTATAGGCCCCCGGAGTTGCTGCTTGGAGCCACAAAGTATGGTCCAGCTGTTGATATGTGGTCTGTTGGTTGCATCTTTGCTGAACTCCTGCATGGAAAGCCAATTTTACAAGGAAGAAATGAACCTGACCAACTGAACAAGATATTCGAGCTTTGTGGATCACCTGATGAGATCAACTGGCCAGATGTTTCAAAACTTCCTTGGTATAATAATCTCAAGCCAACAAAAACCATGAAACGATGTGTTGGGGAAATTTTCGGACATTTTGACTTTCATGCTTTGGATTTACTGGACCACTTATTAACTCTTGATCCAAATCAGAGATGGACTGCGAAGCAAGCGCTGAATGCAGATTATTTCTGGGCTGACCCTTTACCCTGTGATCCAAAGAGCCTGCCTAAGTATGAATCGTCACATGAATTCCAAACAAAGAAAAGGAAGCAGCAGCAGCAGCATCAAACTGAAGACGTATCAAAGAGATCAAAGTCACAGCATTCTCATAAGAATGATTGCCCGCCTCCCATTCAACATACCGTGCAAGCTCCTCCACAATTGCAGGGGTCCCATCATCCGATGACTGATAACCAGCCCCCATTATCTGCTGGACCTAGCCACCACCACTATGGAAAGCCGCACGGTCTTCCTGGAGGCCCAAGCAGGTATCCTCCCAGTGGAAACCAGAGTGGGCCTTACCATATGAATCGGGGAGATCAAGCAGGAGGTTACAGTGGCAGACCATATCCACCCCAAGGACGTGCTCCACCATATGCTGGGAGTGGACCAAGTGCTCCTAGCACAGGAGGCGCCCCTGCTGGTTATGGAATTGGCCCTCCAAACTATTCCCAAAACAGTCAATATGGAGTTCCTGCTGCTGATAGAGGTATGAGTACACGAAATGAACAGTATGGTCGGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0065007 GO:0071704 GO:0097472 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

530

Amino Acids

59.1

Weight (kDa)

8.72

Isoelectric Point (pI)

40.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	25 - 344	5.3e-70	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	27 - 95	1.5e-07	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	130 - 269	5.5e-18	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002129)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10270 AT5G64960 AT5G64960
fragaria_vesca	FvH4_1g20740 FvH4_1g20740 FvH4_1g20740 FvH4_1g20740 FvH4_4g11370 FvH4_4g11370 FvH4_4g11370
malus_domestica	MD04G1037600.v1.1 MD15G1318300.v1.1
prunus_persica	Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.1G186900_v2.0.a1 Prupe.6G187600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0113361 RchiOBHm_Chr2g0113381 RchiOBHm_Chr4g0407881
rosa_laevigata	RLG00000008692 RLG00000018058
rosa_multiflora	Rmu_sc0002073.1_g000029 Rmu_sc0007621.1_g000001 Rmu_sc0011967.1_g000006 Rmu_sc0029075.1_g000002 Rmu_sc0029867.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129320 Rroxscaffold_2G00129850 Rroxscaffold_5G00352420
rosa_rugosa	Rorug02G0186500 Rorug04G0081800 Rorug04G0081900
rosa_samantha	Rh2AG241100 Rh2BG255300 Rh2CG245400 Rh2DG249100 Rh4AG147000 Rh4BG144500 Rh4CG153800 Rh4DG141000
rosa_wichuraiana	Rw2G018830 Rw4G012070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	272, 1320
AccB1I	GGYRCC	1 cut(s)	1475
AccB7I	CCANNNNNTGG	1 cut(s)	1442
AccI	GTMKAC	1 cut(s)	66
AciI	CCGC	2 cut(s)	1187, 1304
AclWI	GGATC	4 cut(s)	429, 838, 977, 1049
AcoI	YGGCCR	2 cut(s)	123, 851
AcsI	RAATTY	3 cut(s)	222, 924, 1091
AcuI	CTGAAG	3 cut(s)	248, 281, 1155
AcyI	GRCGYC	1 cut(s)	1476
AfaI	GTAC	3 cut(s)	416, 537, 1561
AfeI	AGCGCT	1 cut(s)	1014
AfiI	CCNNNNNNNGG	5 cut(s)	141, 1343, 1421, 1442, 1469
AgsI	TTSAA	4 cut(s)	160, 592, 864, 1199
AhlI	ACTAGT	1 cut(s)	377
AjiI	CACGTC	1 cut(s)	1427
AjnI	CCWGG	2 cut(s)	16, 1315
AluBI	AGCT	7 cut(s)	235, 259, 301, 595, 719, 823, 1214
AluI	AGCT	7 cut(s)	235, 259, 301, 595, 719, 823, 1214
Alw21I	GWGCWC	3 cut(s)	90, 1432, 1462
AlwI	GGATC	4 cut(s)	429, 838, 977, 1049
AlwNI	CAGNNNCTG	1 cut(s)	17
Ama87I	CYCGRG	1 cut(s)	83
Aor51HI	AGCGCT	1 cut(s)	1014
AoxI	GGCC	7 cut(s)	123, 436, 677, 851, 1321, 1358, 1498
ApeKI	GCWGC	9 cut(s)	5, 8, 11, 691, 1114, 1117, 1120, 1123, 1541
ApoI	RAATTY	3 cut(s)	222, 924, 1091
AspLEI	GCGC	2 cut(s)	1015, 1478
AsuC2I	CCSGG	2 cut(s)	212, 683
AsuHPI	GGTGA	2 cut(s)	533, 825
AsuNHI	GCTAGC	1 cut(s)	595
AvaI	CYCGRG	1 cut(s)	83
AvaII	GGWCC	7 cut(s)	284, 344, 713, 964, 1233, 1277, 1451
BalI	TGGCCA	1 cut(s)	853
BanI	GGYRCC	1 cut(s)	1475
BanII	GRGCYC	1 cut(s)	513
BauI	CACGAG	1 cut(s)	635
BbsI	GAAGAC	2 cut(s)	1143, 1304
Bbv12I	GWGCWC	3 cut(s)	90, 1432, 1462
BbvCI	CCTCAGC	1 cut(s)	302
BbvI	GCAGC	8 cut(s)	20, 23, 678, 1126, 1129, 1132, 1135, 1528
BccI	CCATC	2 cut(s)	990, 1245
BceAI	ACGGC	1 cut(s)	138
BciT130I	CCWGG	2 cut(s)	18, 1317
BciVI	GTATCC	1 cut(s)	1344
BcnI	CCSGG	2 cut(s)	212, 683
BcuI	ACTAGT	1 cut(s)	377
BfaI	CTAG	4 cut(s)	378, 596, 1281, 1464
BfmI	CTRYAG	2 cut(s)	6, 279
BfoI	RGCGCY	2 cut(s)	1016, 1479
BfuAI	ACCTGC	2 cut(s)	272, 1320
BfuI	GTATCC	1 cut(s)	1344
BmcAI	AGTACT	1 cut(s)	537
Bme1390I	CCNGG	4 cut(s)	18, 212, 683, 1317
Bme18I	GGWCC	7 cut(s)	284, 344, 713, 964, 1233, 1277, 1451
BmeT110I	CYCGRG	1 cut(s)	83
BmgBI	CACGTC	1 cut(s)	1427
BmiI	GGNNCC	8 cut(s)	194, 286, 512, 680, 700, 1234, 1235, 1477
BmrFI	CCNGG	4 cut(s)	18, 212, 683, 1317
BmrI	ACTGGG	3 cut(s)	150, 516, 1335
BmsI	GCATC	3 cut(s)	405, 753, 1135
BmtI	GCTAGC	1 cut(s)	599
BmuI	ACTGGG	3 cut(s)	150, 516, 1335
BpiI	GAAGAC	2 cut(s)	1143, 1304
BpmI	CTGGAG	1 cut(s)	1338
Bpu10I	CCTNAGC	1 cut(s)	302
BpuEI	CTTGAG	1 cut(s)	872
BpuMI	CCSGG	2 cut(s)	212, 683
BsaBI	GATNNNNATC	2 cut(s)	426, 987
BsaHI	GRCGYC	1 cut(s)	1476
BsaJI	CCNNGG	5 cut(s)	17, 387, 681, 872, 1420
Bsc4I	CCNNNNNNNGG	5 cut(s)	141, 1343, 1421, 1442, 1469
Bse1I	ACTGG	5 cut(s)	145, 511, 854, 966, 1341
Bse8I	GATNNNNATC	2 cut(s)	426, 987
BseBI	CCWGG	2 cut(s)	18, 1317
BseDI	CCNNGG	5 cut(s)	17, 387, 681, 872, 1420
BseGI	GGATG	3 cut(s)	301, 331, 1240
BseJI	GATNNNNATC	2 cut(s)	426, 987
BseLI	CCNNNNNNNGG	5 cut(s)	141, 1343, 1421, 1442, 1469
BseMII	CTCAG	1 cut(s)	293
BseNI	ACTGG	5 cut(s)	145, 511, 854, 966, 1341
BseRI	GAGGAG	1 cut(s)	1206
BseXI	GCAGC	8 cut(s)	20, 23, 678, 1126, 1129, 1132, 1135, 1528
BseYI	CCCAGC	1 cut(s)	1442
BshFI	GGCC	7 cut(s)	125, 438, 679, 853, 1323, 1360, 1500
BshNI	GGYRCC	1 cut(s)	1475
BsiHKAI	GWGCWC	3 cut(s)	90, 1432, 1462
BsiHKCI	CYCGRG	1 cut(s)	83
BsiSI	CCGG	2 cut(s)	211, 683
BslFI	GGGAC	2 cut(s)	270, 1219
BslI	CCNNNNNNNGG	5 cut(s)	141, 1343, 1421, 1442, 1469
BsmFI	GGGAC	2 cut(s)	270, 1219
BsmI	GAATGC	2 cut(s)	1024, 1165
BsnI	GGCC	7 cut(s)	125, 438, 679, 853, 1323, 1360, 1500
BsoBI	CYCGRG	1 cut(s)	83
Bsp1286I	GDGCHC	4 cut(s)	90, 513, 1432, 1462
Bsp143I	GATC	8 cut(s)	421, 427, 830, 843, 982, 1054, 1151, 1381
BspACI	CCGC	2 cut(s)	1187, 1304
BspANI	GGCC	7 cut(s)	125, 438, 679, 853, 1323, 1360, 1500
BspCNI	CTCAG	1 cut(s)	294
BspHI	TCATGA	2 cut(s)	241, 424
BspLI	GGNNCC	8 cut(s)	194, 286, 512, 680, 700, 1234, 1235, 1477
BspMAI	CTGCAG	2 cut(s)	10, 283
BspMI	ACCTGC	2 cut(s)	272, 1320
BspOI	GCTAGC	1 cut(s)	599
BspPI	GGATC	4 cut(s)	429, 838, 977, 1049
BspT107I	GGYRCC	1 cut(s)	1475
BsrI	ACTGG	5 cut(s)	145, 511, 854, 966, 1341
BssECI	CCNNGG	5 cut(s)	17, 387, 681, 872, 1420
BssMI	GATC	8 cut(s)	421, 427, 830, 843, 982, 1054, 1151, 1381
BssNI	GRCGYC	1 cut(s)	1476
BssSI	CACGAG	1 cut(s)	635
BssT1I	CCWWGG	3 cut(s)	387, 872, 1420
Bst2BI	CACGAG	1 cut(s)	635
Bst2UI	CCWGG	2 cut(s)	18, 1317
BstACI	GRCGYC	1 cut(s)	1476
BstC8I	GCNNGC	6 cut(s)	597, 612, 1011, 1067, 1187, 1212
BstDEI	CTNAG	2 cut(s)	302, 1071
BstENI	CCTNNNNNAGG	1 cut(s)	1467
BstF5I	GGATG	3 cut(s)	301, 331, 1240
BstH2I	RGCGCY	2 cut(s)	1016, 1479
BstHHI	GCGC	2 cut(s)	1015, 1478
BstKTI	GATC	8 cut(s)	424, 430, 833, 846, 985, 1057, 1154, 1384
BstMBI	GATC	8 cut(s)	421, 427, 830, 843, 982, 1054, 1151, 1381
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 69, 769, 1010, 1019, 1120, 1123, 1473
BstNI	CCWGG	2 cut(s)	18, 1317
BstSCI	CCNGG	4 cut(s)	16, 210, 681, 1315
BstSFI	CTRYAG	2 cut(s)	6, 279
BstV1I	GCAGC	8 cut(s)	20, 23, 678, 1126, 1129, 1132, 1135, 1528
BstV2I	GAAGAC	2 cut(s)	1143, 1304
BstXI	CCANNNNNNTGG	1 cut(s)	1295
BsuI	GTATCC	1 cut(s)	1344
BsuRI	GGCC	7 cut(s)	125, 438, 679, 853, 1323, 1360, 1500
BtrI	CACGTC	1 cut(s)	1427
BtsCI	GGATG	3 cut(s)	301, 331, 1240
BtsIMutI	CAGTG	2 cut(s)	1348, 1405
BveI	ACCTGC	2 cut(s)	272, 1320
Cac8I	GCNNGC	6 cut(s)	597, 612, 1011, 1067, 1187, 1212
CaiI	CAGNNNCTG	1 cut(s)	17
CciI	TCATGA	2 cut(s)	241, 424
CfoI	GCGC	2 cut(s)	1015, 1478
CseI	GACGC	1 cut(s)	52
Csp6I	GTAC	3 cut(s)	415, 536, 1560
CviAII	CATG	9 cut(s)	172, 242, 418, 425, 524, 764, 904, 947, 1088
CviQI	GTAC	3 cut(s)	415, 536, 1560
DdeI	CTNAG	2 cut(s)	302, 1071
DinI	GGCGCC	1 cut(s)	1477
DpnI	GATC	8 cut(s)	423, 429, 832, 845, 984, 1056, 1153, 1383
DpnII	GATC	8 cut(s)	421, 427, 830, 843, 982, 1054, 1151, 1381
EaeI	YGGCCR	2 cut(s)	123, 851
Eco130I	CCWWGG	3 cut(s)	387, 872, 1420
Eco147I	AGGCCT	1 cut(s)	438
Eco24I	GRGCYC	1 cut(s)	513
Eco47I	GGWCC	7 cut(s)	284, 344, 713, 964, 1233, 1277, 1451
Eco47III	AGCGCT	1 cut(s)	1014
Eco57I	CTGAAG	3 cut(s)	248, 281, 1155
Eco88I	CYCGRG	1 cut(s)	83
EcoNI	CCTNNNNNAGG	1 cut(s)	1467
EcoO109I	RGGNCCY	4 cut(s)	284, 344, 678, 1233
EcoRI	GAATTC	1 cut(s)	1091
EcoRII	CCWGG	2 cut(s)	16, 1315
EcoT14I	CCWWGG	3 cut(s)	387, 872, 1420
EcoT38I	GRGCYC	1 cut(s)	513
EgeI	GGCGCC	1 cut(s)	1477
EheI	GGCGCC	1 cut(s)	1477
ErhI	CCWWGG	3 cut(s)	387, 872, 1420
FaeI	CATG	9 cut(s)	175, 245, 421, 428, 527, 767, 907, 950, 1091
FaqI	GGGAC	2 cut(s)	270, 1219
FatI	CATG	9 cut(s)	171, 241, 417, 424, 523, 763, 903, 946, 1087
FauI	CCCGC	1 cut(s)	1194
FauNDI	CATATG	2 cut(s)	1368, 1438
FblI	GTMKAC	1 cut(s)	66
FokI	GGATG	3 cut(s)	308, 338, 1227
FriOI	GRGCYC	1 cut(s)	513
FspBI	CTAG	4 cut(s)	378, 596, 1281, 1464
GlaI	GCGC	2 cut(s)	1014, 1477
GsaI	CCCAGC	1 cut(s)	1446
GsuI	CTGGAG	1 cut(s)	1338
HaeII	RGCGCY	2 cut(s)	1016, 1479
HaeIII	GGCC	7 cut(s)	125, 438, 679, 853, 1323, 1360, 1500
HapII	CCGG	2 cut(s)	211, 683
HgaI	GACGC	1 cut(s)	52
HhaI	GCGC	2 cut(s)	1015, 1478
Hin1I	GRCGYC	1 cut(s)	1476
Hin1II	CATG	9 cut(s)	175, 245, 421, 428, 527, 767, 907, 950, 1091
Hin6I	GCGC	2 cut(s)	1013, 1476
HinP1I	GCGC	2 cut(s)	1013, 1476
HincII	GTYRAC	1 cut(s)	67
HindII	GTYRAC	1 cut(s)	67
HindIII	AAGCTT	1 cut(s)	233
HinfI	GANTC	5 cut(s)	307, 337, 367, 1079, 1372
HpaII	CCGG	2 cut(s)	211, 683
HphI	GGTGA	2 cut(s)	533, 825
Hpy166II	GTNNAC	4 cut(s)	67, 120, 1451, 1562
Hpy188I	TCNGA	6 cut(s)	228, 432, 932, 993, 1245, 1584
Hpy188III	TCNNGA	9 cut(s)	57, 242, 287, 317, 347, 371, 425, 453, 980
Hpy8I	GTNNAC	4 cut(s)	67, 120, 1451, 1562
Hpy99I	CGWCG	1 cut(s)	68
HpyAV	CCTTC	3 cut(s)	92, 182, 256
HpyCH4IV	ACGT	2 cut(s)	1140, 1426
HpyCH4V	TGCA	8 cut(s)	8, 281, 614, 744, 763, 1022, 1210, 1228
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 69, 769, 1010, 1019, 1120, 1123, 1473
HpyF3I	CTNAG	2 cut(s)	302, 1071
HpySE526I	ACGT	2 cut(s)	1140, 1426
Hsp92I	GRCGYC	1 cut(s)	1476
Hsp92II	CATG	9 cut(s)	175, 245, 421, 428, 527, 767, 907, 950, 1091
HspAI	GCGC	2 cut(s)	1013, 1476
KasI	GGCGCC	1 cut(s)	1475
KflI	GGGWCCC	1 cut(s)	1233
Kzo9I	GATC	8 cut(s)	421, 427, 830, 843, 982, 1054, 1151, 1381
LmnI	GCTCC	5 cut(s)	516, 698, 1219, 1435, 1465
Lsp1109I	GCAGC	8 cut(s)	20, 23, 678, 1126, 1129, 1132, 1135, 1528
LweI	GCATC	3 cut(s)	405, 753, 1135
MaeI	CTAG	4 cut(s)	378, 596, 1281, 1464
MaeII	ACGT	2 cut(s)	1140, 1426
MaeIII	GTNAC	6 cut(s)	271, 545, 629, 1083, 1158, 1394
MalI	GATC	8 cut(s)	423, 429, 832, 845, 984, 1056, 1153, 1383
MboI	GATC	8 cut(s)	421, 427, 830, 843, 982, 1054, 1151, 1381
MboII	GAAGA	4 cut(s)	241, 798, 1148, 1304
MfeI	CAATTG	1 cut(s)	1223
MhlI	GDGCHC	4 cut(s)	90, 513, 1432, 1462
MlsI	TGGCCA	1 cut(s)	853
MluCI	AATT	9 cut(s)	42, 147, 216, 222, 774, 924, 1091, 1223, 1494
MluNI	TGGCCA	1 cut(s)	853
Mly113I	GGCGCC	1 cut(s)	1476
MmeI	TCCRAC	1 cut(s)	1562
Mox20I	TGGCCA	1 cut(s)	853
MscI	TGGCCA	1 cut(s)	853
MseI	TTAA	6 cut(s)	41, 75, 219, 255, 528, 974
MslI	CAYNNNNRTG	4 cut(s)	246, 401, 1176, 1293
Msp20I	TGGCCA	1 cut(s)	853
MspA1I	CMGCKG	1 cut(s)	719
MspI	CCGG	2 cut(s)	211, 683
MspR9I	CCNGG	4 cut(s)	18, 212, 683, 1317
MunI	CAATTG	1 cut(s)	1223
Mva1269I	GAATGC	2 cut(s)	1024, 1165
MvaI	CCWGG	2 cut(s)	18, 1317
MwoI	GCNNNNNNNGC	8 cut(s)	11, 69, 769, 1010, 1019, 1120, 1123, 1473
NarI	GGCGCC	1 cut(s)	1476
NciI	CCSGG	2 cut(s)	212, 683
NdeI	CATATG	2 cut(s)	1368, 1438
NdeII	GATC	8 cut(s)	421, 427, 830, 843, 982, 1054, 1151, 1381
NheI	GCTAGC	1 cut(s)	595
NlaIII	CATG	9 cut(s)	175, 245, 421, 428, 527, 767, 907, 950, 1091
NlaIV	GGNNCC	8 cut(s)	194, 286, 512, 680, 700, 1234, 1235, 1477
NmuCI	GTSAC	3 cut(s)	545, 1083, 1158
PaeR7I	CTCGAG	1 cut(s)	83
PagI	TCATGA	2 cut(s)	241, 424
PceI	AGGCCT	1 cut(s)	438
PctI	GAATGC	2 cut(s)	1024, 1165
PfeI	GAWTC	5 cut(s)	307, 337, 367, 1079, 1372
PflMI	CCANNNNNTGG	1 cut(s)	1442
PfoI	TCCNGGA	2 cut(s)	210, 1315
PluTI	GGCGCC	1 cut(s)	1479
PpuMI	RGGWCCY	3 cut(s)	284, 344, 1233
Psp5II	RGGWCCY	3 cut(s)	284, 344, 1233
Psp6I	CCWGG	2 cut(s)	16, 1315
PspFI	CCCAGC	1 cut(s)	1442
PspGI	CCWGG	2 cut(s)	16, 1315
PspN4I	GGNNCC	8 cut(s)	194, 286, 512, 680, 700, 1234, 1235, 1477
PspPPI	RGGWCCY	3 cut(s)	284, 344, 1233
PspXI	VCTCGAGB	1 cut(s)	83
PstI	CTGCAG	2 cut(s)	10, 283
PstNI	CAGNNNCTG	1 cut(s)	17
PvuII	CAGCTG	1 cut(s)	719
RsaI	GTAC	3 cut(s)	416, 537, 1561
RsaNI	GTAC	3 cut(s)	415, 536, 1560
RseI	CAYNNNNRTG	4 cut(s)	246, 401, 1176, 1293
SalI	GTCGAC	1 cut(s)	65
SaqAI	TTAA	6 cut(s)	41, 75, 219, 255, 528, 974
Sau3AI	GATC	8 cut(s)	421, 427, 830, 843, 982, 1054, 1151, 1381
ScaI	AGTACT	1 cut(s)	537
ScrFI	CCNGG	4 cut(s)	18, 212, 683, 1317
SduI	GDGCHC	4 cut(s)	90, 513, 1432, 1462
SfaNI	GCATC	3 cut(s)	405, 753, 1135
SfcI	CTRYAG	2 cut(s)	6, 279
SfoI	GGCGCC	1 cut(s)	1477
Sfr274I	CTCGAG	1 cut(s)	83
SinI	GGWCC	7 cut(s)	284, 344, 713, 964, 1233, 1277, 1451
SlaI	CTCGAG	1 cut(s)	83
SmiMI	CAYNNNNRTG	4 cut(s)	246, 401, 1176, 1293
SmlI	CTYRAG	2 cut(s)	83, 887
SmoI	CTYRAG	2 cut(s)	83, 887
SpeI	ACTAGT	1 cut(s)	377
Sse9I	AATT	9 cut(s)	42, 147, 216, 222, 774, 924, 1091, 1223, 1494
SseBI	AGGCCT	1 cut(s)	438
SsiI	CCGC	2 cut(s)	1187, 1304
SspDI	GGCGCC	1 cut(s)	1475
SspMI	CTAG	4 cut(s)	378, 596, 1281, 1464
StuI	AGGCCT	1 cut(s)	438
StyD4I	CCNGG	4 cut(s)	16, 210, 681, 1315
StyI	CCWWGG	3 cut(s)	387, 872, 1420
TaiI	ACGT	2 cut(s)	1143, 1429
TaqI	TCGA	4 cut(s)	58, 66, 84, 819
TasI	AATT	9 cut(s)	42, 147, 216, 222, 774, 924, 1091, 1223, 1494
TatI	WGTACW	3 cut(s)	414, 535, 1559
TauI	GCSGC	1 cut(s)	1306
TfiI	GAWTC	5 cut(s)	307, 337, 367, 1079, 1372
Tru1I	TTAA	6 cut(s)	41, 75, 219, 255, 528, 974
Tru9I	TTAA	6 cut(s)	41, 75, 219, 255, 528, 974
TscAI	CASTG	2 cut(s)	1348, 1405
TseFI	GTSAC	3 cut(s)	545, 1083, 1158
TseI	GCWGC	9 cut(s)	5, 8, 11, 691, 1114, 1117, 1120, 1123, 1541
Tsp45I	GTSAC	3 cut(s)	545, 1083, 1158
TspRI	CASTG	2 cut(s)	1348, 1405
Van91I	CCANNNNNTGG	1 cut(s)	1442
VpaK11BI	GGWCC	7 cut(s)	284, 344, 713, 964, 1233, 1277, 1451
XagI	CCTNNNNNAGG	1 cut(s)	1467
XapI	RAATTY	3 cut(s)	222, 924, 1091
XcmI	CCANNNNNNNNNTGG	2 cut(s)	993, 1292
XhoI	CTCGAG	1 cut(s)	83
XmiI	GTMKAC	1 cut(s)	66
XspI	CTAG	4 cut(s)	378, 596, 1281, 1464
ZrmI	AGTACT	1 cut(s)	537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.