Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003365

Physical Location & Seq

Genomic Coordinates Forward (+)

5227747 .. 5229117

Gene Structure 1371 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G16010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 807 bp

ATGGAAATGGTGACAAGTTTTGTATATCCACCACCTCCATCAATATTGCTCAATTGTATGAGCGTCGTTGGTGTAGCTGCTCTGGCCAATATCGGTTGGTCTGAAATCAGAGGAAATCATCTCAAATACTCCAAATTCGGCGTATCATCATCATCACCACAACCACAGAAGGAGCGATTCGGCAGCATCTCAAGCCGAAATGGAATGCTTTTGCTATACACACCTGCGTTTCTAGCAGCTGCGTCTTCTTTCTTCGTCGTACCTTCTGATGATCTCAGGTTTCTTCTTCTCAAATCCGCACTTGCCCTCCATTTCTTCAAGAGGGTATTCGAGGTTCTGTTCATACATAAATACAGTGGAGGGATGGCTATAGACTCAGCTCTCACCATAAGTAGCAGCTATTTCTCATCCACAGCATTGATGTTATACAGTCAAAATCTCACCTTGGGACTTACCGAGCCGAGTTTCGATATGAAACTCGCCGGAGTTGTGATGTTTGTGGTGGGAATTGTTGGGAATCTGTATCACCATGTTTTGCTGGCTAAGCTAAGGAAAGAAGATGGGAAGAAAGAGTACAAGATACCAAAAGGAGGTCTCTTTGATATAATCATATGCCCTCACTATCTATTTGAGATCCTTGTGTTTTGGAGCTTCTTTCTCATCTCTCAGACCATTTATTCATTTTCTTTCGCTATGGGAACAATGCTCTATCTCATTGGTCGGAGTTATGCTACAAGAACTTGGTATCTTTCCAAGTTTGATGACTTCCCCAAGCATATCAAGGCTCTCATTCCCTTTGTATTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0006702 GO:0007154 GO:0007267 GO:0007275 GO:0007548 GO:0008150 GO:0008152 GO:0008202 GO:0008207 GO:0008209 GO:0008406 GO:0008584 GO:0008610 GO:0009058 GO:0009917 GO:0009987 GO:0010817 GO:0012505 GO:0016020 GO:0016229 GO:0016491 GO:0016614 GO:0016616 GO:0016627 GO:0017144 GO:0022414 GO:0023052 GO:0030283 GO:0030539 GO:0030540 GO:0031984 GO:0032501 GO:0032502 GO:0033764 GO:0033765 GO:0034754 GO:0042175 GO:0042180 GO:0042181 GO:0042445 GO:0042446 GO:0042448 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045137 GO:0046546 GO:0046660 GO:0046661 GO:0048513 GO:0048608 GO:0048731 GO:0048806 GO:0048856 GO:0055114 GO:0061370 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00062 ko00140 ko01040 ko01110 ko01212 map00062 map00140 map01040 map01110 map01212

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

268

Amino Acids

30.13

Weight (kDa)

9.38

Isoelectric Point (pI)

45.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	158 - 268	1.3e-27	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003365)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16010
fragaria_vesca	FvH4_2g07871 FvH4_2g07871 FvH4_2g07872 FvH4_2g07900
malus_domestica	MD02G1261000.v1.1 MD02G1265600.v1.1 MD07G1060000.v1.1
prunus_persica	Prupe.2G066100_v2.0.a1 Prupe.2G066100_v2.0.a1 Prupe.2G066300_v2.0.a1 Prupe.2G066400_v2.0.a1 Prupe.2G066400_v2.0.a1
pyrus_communis	pycom02g22310 pycom02g22710
rosa_chinensis	RchiOBHm_Chr6g0262341 RchiOBHm_Chr6g0262351
rosa_laevigata	RLG00000014437 RLG00000014438
rosa_multiflora	Rmu_sc0001544.1_g000021 Rmu_sc0001544.1_g000027 Rmu_sc0028284.1_g000001
rosa_roxburghii	Rroxscaffold_7G00207990
rosa_rugosa	Rorug05G0596300 Rorug05G0596400 Rorug05G0596400
rosa_samantha	Rh6AG116700 Rh6AG117000 Rh6BG110800 Rh6CG108300 Rh6CG108400 Rh6CG108600 Rh6DG098800
rosa_wichuraiana	Rw6G010130 Rw6G010150 Rw6G010160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	232
Acc36I	ACCTGC	1 cut(s)	232
AciI	CCGC	1 cut(s)	297
AclWI	GGATC	1 cut(s)	628
AcoI	YGGCCR	1 cut(s)	84
AcsI	RAATTY	1 cut(s)	134
AfaI	GTAC	2 cut(s)	261, 575
AfiI	CCNNNNNNNGG	1 cut(s)	590
AgsI	TTSAA	1 cut(s)	319
AluBI	AGCT	6 cut(s)	77, 239, 380, 399, 547, 651
AluI	AGCT	6 cut(s)	77, 239, 380, 399, 547, 651
Alw26I	GTCTC	1 cut(s)	599
AlwI	GGATC	1 cut(s)	628
AoxI	GGCC	1 cut(s)	84
ApeKI	GCWGC	5 cut(s)	77, 183, 236, 239, 396
ApoI	RAATTY	1 cut(s)	134
AsuHPI	GGTGA	5 cut(s)	22, 147, 376, 433, 518
BalI	TGGCCA	1 cut(s)	86
BarI	GAAGNNNNNNTAC	2 cut(s)	557, 589
BbsI	GAAGAC	1 cut(s)	237
BbvI	GCAGC	5 cut(s)	64, 195, 226, 248, 408
BccI	CCATC	3 cut(s)	46, 358, 554
BcoDI	GTCTC	1 cut(s)	599
BfaI	CTAG	2 cut(s)	233, 805
BfmI	CTRYAG	1 cut(s)	369
BfuAI	ACCTGC	1 cut(s)	232
BisI	GCNGC	5 cut(s)	78, 184, 237, 240, 397
BlpI	GCTNAGC	1 cut(s)	543
BlsI	GCNGC	5 cut(s)	79, 185, 238, 241, 398
BmsI	GCATC	1 cut(s)	195
BpiI	GAAGAC	1 cut(s)	237
Bpu10I	CCTNAGC	1 cut(s)	548
Bpu1102I	GCTNAGC	1 cut(s)	543
BpuEI	CTTGAG	1 cut(s)	175
BsaI	GGTCTC	1 cut(s)	599
BsaJI	CCNNGG	1 cut(s)	444
BsaXI	ACNNNNNCTCC	2 cut(s)	291, 321
Bsc4I	CCNNNNNNNGG	1 cut(s)	590
BseDI	CCNNGG	1 cut(s)	444
BseGI	GGATG	2 cut(s)	369, 407
BseLI	CCNNNNNNNGG	1 cut(s)	590
BseMII	CTCAG	3 cut(s)	289, 390, 680
BseXI	GCAGC	5 cut(s)	64, 195, 226, 248, 408
BshFI	GGCC	1 cut(s)	86
BsiSI	CCGG	1 cut(s)	483
BslFI	GGGAC	1 cut(s)	462
BslI	CCNNNNNNNGG	1 cut(s)	590
BsmAI	GTCTC	1 cut(s)	599
BsmFI	GGGAC	1 cut(s)	462
BsmI	GAATGC	1 cut(s)	210
BsnI	GGCC	1 cut(s)	86
Bso31I	GGTCTC	1 cut(s)	599
Bsp143I	GATC	2 cut(s)	271, 633
Bsp1720I	GCTNAGC	1 cut(s)	543
BspACI	CCGC	1 cut(s)	297
BspANI	GGCC	1 cut(s)	86
BspCNI	CTCAG	3 cut(s)	288, 389, 679
BspMI	ACCTGC	1 cut(s)	232
BspPI	GGATC	1 cut(s)	628
BspTNI	GGTCTC	1 cut(s)	599
BssECI	CCNNGG	1 cut(s)	444
BssMI	GATC	2 cut(s)	271, 633
BssT1I	CCWWGG	1 cut(s)	444
Bst4CI	ACNGT	2 cut(s)	356, 431
BstC8I	GCNNGC	1 cut(s)	540
BstDEI	CTNAG	5 cut(s)	275, 376, 543, 548, 666
BstF5I	GGATG	2 cut(s)	369, 407
BstKTI	GATC	2 cut(s)	274, 636
BstMAI	GTCTC	1 cut(s)	599
BstMBI	GATC	2 cut(s)	271, 633
BstMWI	GCNNNNNNNGC	4 cut(s)	83, 192, 233, 544
BstSFI	CTRYAG	1 cut(s)	369
BstV1I	GCAGC	5 cut(s)	64, 195, 226, 248, 408
BstV2I	GAAGAC	1 cut(s)	237
BstX2I	RGATCY	1 cut(s)	633
BstYI	RGATCY	1 cut(s)	633
BsuRI	GGCC	1 cut(s)	86
BtsCI	GGATG	2 cut(s)	369, 407
BtsIMutI	CAGTG	1 cut(s)	361
BveI	ACCTGC	1 cut(s)	232
Cac8I	GCNNGC	1 cut(s)	540
CseI	GACGC	2 cut(s)	52, 231
Csp6I	GTAC	2 cut(s)	260, 574
CviAII	CATG	1 cut(s)	530
CviQI	GTAC	2 cut(s)	260, 574
DdeI	CTNAG	5 cut(s)	275, 376, 543, 548, 666
DpnI	GATC	2 cut(s)	273, 635
DpnII	GATC	2 cut(s)	271, 633
EaeI	YGGCCR	1 cut(s)	84
Eco130I	CCWWGG	1 cut(s)	444
Eco31I	GGTCTC	1 cut(s)	599
EcoT14I	CCWWGG	1 cut(s)	444
ErhI	CCWWGG	1 cut(s)	444
FaeI	CATG	1 cut(s)	533
FaqI	GGGAC	1 cut(s)	462
FatI	CATG	1 cut(s)	529
FauNDI	CATATG	1 cut(s)	611
Fnu4HI	GCNGC	5 cut(s)	78, 184, 237, 240, 397
FokI	GGATG	2 cut(s)	376, 394
Fsp4HI	GCNGC	5 cut(s)	78, 184, 237, 240, 397
FspBI	CTAG	2 cut(s)	233, 805
GluI	GCNGC	5 cut(s)	78, 184, 237, 240, 397
HaeIII	GGCC	1 cut(s)	86
HapII	CCGG	1 cut(s)	483
HgaI	GACGC	2 cut(s)	52, 231
Hin1II	CATG	1 cut(s)	533
HinfI	GANTC	3 cut(s)	177, 374, 517
HpaII	CCGG	1 cut(s)	483
HphI	GGTGA	5 cut(s)	22, 147, 376, 433, 518
Hpy188I	TCNGA	5 cut(s)	103, 110, 268, 669, 723
Hpy188III	TCNNGA	1 cut(s)	319
Hpy99I	CGWCG	2 cut(s)	68, 260
HpyAV	CCTTC	2 cut(s)	163, 273
HpyCH4III	ACNGT	2 cut(s)	356, 431
HpyF10VI	GCNNNNNNNGC	4 cut(s)	83, 192, 233, 544
HpyF3I	CTNAG	5 cut(s)	275, 376, 543, 548, 666
Hsp92II	CATG	1 cut(s)	533
Kzo9I	GATC	2 cut(s)	271, 633
LmnI	GCTCC	2 cut(s)	172, 648
LpnPI	CCDG	5 cut(s)	68, 237, 262, 496, 524
Lsp1109I	GCAGC	5 cut(s)	64, 195, 226, 248, 408
LweI	GCATC	1 cut(s)	195
MaeI	CTAG	2 cut(s)	233, 805
MaeIII	GTNAC	1 cut(s)	10
MalI	GATC	2 cut(s)	273, 635
MboI	GATC	2 cut(s)	271, 633
MboII	GAAGA	7 cut(s)	237, 244, 275, 278, 307, 569, 577
MfeI	CAATTG	1 cut(s)	52
MflI	RGATCY	1 cut(s)	633
MlsI	TGGCCA	1 cut(s)	86
MluCI	AATT	3 cut(s)	52, 134, 507
MluNI	TGGCCA	1 cut(s)	86
MlyI	GAGTC	1 cut(s)	368
MmeI	TCCRAC	1 cut(s)	701
MnlI	CCTC	8 cut(s)	45, 104, 315, 317, 325, 353, 584, 627
Mox20I	TGGCCA	1 cut(s)	86
MscI	TGGCCA	1 cut(s)	86
Msp20I	TGGCCA	1 cut(s)	86
MspA1I	CMGCKG	1 cut(s)	239
MspI	CCGG	1 cut(s)	483
MunI	CAATTG	1 cut(s)	52
Mva1269I	GAATGC	1 cut(s)	210
MwoI	GCNNNNNNNGC	4 cut(s)	83, 192, 233, 544
NdeI	CATATG	1 cut(s)	611
NdeII	GATC	2 cut(s)	271, 633
NlaIII	CATG	1 cut(s)	533
NmeAIII	GCCGAG	1 cut(s)	486
NmuCI	GTSAC	1 cut(s)	10
PaqCI	CACCTGC	1 cut(s)	232
PctI	GAATGC	1 cut(s)	210
PfeI	GAWTC	2 cut(s)	177, 517
PkrI	GCNGC	5 cut(s)	79, 185, 238, 241, 398
PleI	GAGTC	1 cut(s)	368
PpsI	GAGTC	1 cut(s)	368
PsrI	GAACNNNNNNTAC	2 cut(s)	318, 350
PsuI	RGATCY	1 cut(s)	633
PvuII	CAGCTG	1 cut(s)	239
RsaI	GTAC	2 cut(s)	261, 575
RsaNI	GTAC	2 cut(s)	260, 574
SatI	GCNGC	5 cut(s)	78, 184, 237, 240, 397
Sau3AI	GATC	2 cut(s)	271, 633
SchI	GAGTC	1 cut(s)	368
SfaNI	GCATC	1 cut(s)	195
SfcI	CTRYAG	1 cut(s)	369
SmlI	CTYRAG	1 cut(s)	190
SmoI	CTYRAG	1 cut(s)	190
Sse9I	AATT	3 cut(s)	52, 134, 507
SsiI	CCGC	1 cut(s)	297
SspI	AATATT	1 cut(s)	45
SspMI	CTAG	2 cut(s)	233, 805
StyI	CCWWGG	1 cut(s)	444
TaaI	ACNGT	2 cut(s)	356, 431
TaqI	TCGA	2 cut(s)	330, 468
TasI	AATT	3 cut(s)	52, 134, 507
TatI	WGTACW	1 cut(s)	573
TfiI	GAWTC	2 cut(s)	177, 517
TscAI	CASTG	1 cut(s)	361
TseFI	GTSAC	1 cut(s)	10
TseI	GCWGC	5 cut(s)	77, 183, 236, 239, 396
Tsp45I	GTSAC	1 cut(s)	10
TspDTI	ATGAA	3 cut(s)	331, 488, 669
TspRI	CASTG	1 cut(s)	361
XapI	RAATTY	1 cut(s)	134
XspI	CTAG	2 cut(s)	233, 805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.