Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003365

Physical Location & Seq

Genomic Coordinates Forward (+)

5587728 .. 5589604

Gene Structure 1877 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1060000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 702 bp

ATGATGGTGGTGAGTAGTATGTCGATAATCTCGACGCTGATTTTCCAACCACCTCCTTCTATATTCATGACAGCAATGTCAGTGATTAGCTTCACGTCATTAGCAAATGCTGGGATTTCAGAGATTAGAGGAAAGCACATGCAGTATTCTAAGTTCAGGAATTTCAATAATCCTCAAAAATCTGCACTAAATTCAGAAAAGCAACAACAAGTAGTAACATTGTCCGGTAGAACAGGCATGCTCGTCGCTTATACTCCTGCCTTCGTCGCTGCTCTCACATCCTTGCTGTTTTTTCCCCATCAAGATATCAGATCTATATTGCTCTCTTACGCTCTAACCCTTCATTTTCTCAAAAGGATTCTTGAGGTTCAATTTGTTCACAAGTACAGTGGAGGAATGGGCGTTGATACAGCACATCACTACCTTCTTTCGGAGATGAGGGTGAAGCCAGGTGAAGAGAAGGAATACAAGCTTCCCAAGGGTGGTTTGTTTGAGCTTGTTATATGCCCTCACTATCTCTTTGAAATCACAACATTTCTTGGGATATCTTTCATTTCTCAGACTCTCTACTCCTTTTCCTTTACCGCTGGAACCGCCCTCTACTTGGCCGGAAGGAGCTATGCTACCAGGAATTGGTATCGTTCCAAGTTTAAATATCATTTTCCCAATAATCTCAAGGCTCTCATTCCATATGTTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0006702 GO:0007154 GO:0007267 GO:0007275 GO:0007548 GO:0008150 GO:0008152 GO:0008202 GO:0008207 GO:0008209 GO:0008406 GO:0008584 GO:0008610 GO:0009058 GO:0009917 GO:0009987 GO:0010817 GO:0012505 GO:0016020 GO:0016229 GO:0016491 GO:0016614 GO:0016616 GO:0016627 GO:0017144 GO:0022414 GO:0023052 GO:0030283 GO:0030539 GO:0030540 GO:0031984 GO:0032501 GO:0032502 GO:0033764 GO:0033765 GO:0034754 GO:0042175 GO:0042180 GO:0042181 GO:0042445 GO:0042446 GO:0042448 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045137 GO:0046546 GO:0046660 GO:0046661 GO:0048513 GO:0048608 GO:0048731 GO:0048806 GO:0048856 GO:0055114 GO:0061370 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00062 ko00140 ko01040 ko01110 ko01212 map00062 map00140 map01040 map01110 map01212

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

234

Amino Acids

26.44

Weight (kDa)

9.73

Isoelectric Point (pI)

39.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	126 - 233	1.3e-19	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003365)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16010
fragaria_vesca	FvH4_2g07871 FvH4_2g07871 FvH4_2g07872 FvH4_2g07900
malus_domestica	MD02G1261000.v1.1 MD02G1265600.v1.1 MD07G1060000.v1.1
prunus_persica	Prupe.2G066100_v2.0.a1 Prupe.2G066100_v2.0.a1 Prupe.2G066300_v2.0.a1 Prupe.2G066400_v2.0.a1 Prupe.2G066400_v2.0.a1
pyrus_communis	pycom02g22310 pycom02g22710
rosa_chinensis	RchiOBHm_Chr6g0262341 RchiOBHm_Chr6g0262351
rosa_laevigata	RLG00000014437 RLG00000014438
rosa_multiflora	Rmu_sc0001544.1_g000021 Rmu_sc0001544.1_g000027 Rmu_sc0028284.1_g000001
rosa_roxburghii	Rroxscaffold_7G00207990
rosa_rugosa	Rorug05G0596300 Rorug05G0596400 Rorug05G0596400
rosa_samantha	Rh6AG116700 Rh6AG117000 Rh6BG110800 Rh6CG108300 Rh6CG108400 Rh6CG108600 Rh6DG098800
rosa_wichuraiana	Rw6G010130 Rw6G010150 Rw6G010160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	76
AccB7I	CCANNNNNTGG	1 cut(s)	633
AciI	CCGC	2 cut(s)	585, 594
AcoI	YGGCCR	1 cut(s)	606
AcsI	RAATTY	2 cut(s)	160, 190
AfaI	GTAC	1 cut(s)	386
AfiI	CCNNNNNNNGG	4 cut(s)	430, 482, 604, 633
AgsI	TTSAA	3 cut(s)	166, 371, 524
AjiI	CACGTC	1 cut(s)	96
AjnI	CCWGG	2 cut(s)	448, 626
AluBI	AGCT	4 cut(s)	90, 472, 496, 618
AluI	AGCT	4 cut(s)	90, 472, 496, 618
AoxI	GGCC	1 cut(s)	606
ApeKI	GCWGC	1 cut(s)	269
ApoI	RAATTY	2 cut(s)	160, 190
AsuHPI	GGTGA	3 cut(s)	22, 454, 464
BbvI	GCAGC	1 cut(s)	256
BccI	CCATC	1 cut(s)	306
BcgI	CGANNNNNNTGC	2 cut(s)	226, 260
BciT130I	CCWGG	2 cut(s)	450, 628
BglII	AGATCT	1 cut(s)	311
BisI	GCNGC	1 cut(s)	270
BlsI	GCNGC	1 cut(s)	271
Bme1390I	CCNGG	2 cut(s)	450, 628
BmgBI	CACGTC	1 cut(s)	96
BmiI	GGNNCC	1 cut(s)	592
BmrFI	CCNGG	2 cut(s)	450, 628
BpuEI	CTTGAG	2 cut(s)	383, 659
BsaJI	CCNNGG	1 cut(s)	477
BsaWI	WCCGGW	1 cut(s)	224
Bsc4I	CCNNNNNNNGG	4 cut(s)	430, 482, 604, 633
Bse3DI	GCAATG	1 cut(s)	81
BseBI	CCWGG	2 cut(s)	450, 628
BseDI	CCNNGG	1 cut(s)	477
BseGI	GGATG	1 cut(s)	278
BseLI	CCNNNNNNNGG	4 cut(s)	430, 482, 604, 633
BseMI	GCAATG	1 cut(s)	81
BseMII	CTCAG	1 cut(s)	572
BseXI	GCAGC	1 cut(s)	256
BseYI	CCCAGC	1 cut(s)	110
BsgI	GTGCAG	1 cut(s)	168
BshFI	GGCC	1 cut(s)	608
BsiSI	CCGG	2 cut(s)	225, 609
BslI	CCNNNNNNNGG	4 cut(s)	430, 482, 604, 633
BsnI	GGCC	1 cut(s)	608
Bsp143I	GATC	1 cut(s)	311
BspACI	CCGC	2 cut(s)	585, 594
BspANI	GGCC	1 cut(s)	608
BspCNI	CTCAG	1 cut(s)	571
BspHI	TCATGA	1 cut(s)	66
BspLI	GGNNCC	1 cut(s)	592
BsrDI	GCAATG	1 cut(s)	81
BssECI	CCNNGG	1 cut(s)	477
BssMI	GATC	1 cut(s)	311
BssT1I	CCWWGG	1 cut(s)	477
Bst2UI	CCWGG	2 cut(s)	450, 628
Bst4CI	ACNGT	1 cut(s)	389
Bst6I	CTCTTC	1 cut(s)	450
BstC8I	GCNNGC	1 cut(s)	239
BstDEI	CTNAG	2 cut(s)	150, 558
BstF5I	GGATG	1 cut(s)	278
BstKTI	GATC	1 cut(s)	314
BstMBI	GATC	1 cut(s)	311
BstMWI	GCNNNNNNNGC	2 cut(s)	266, 593
BstNI	CCWGG	2 cut(s)	450, 628
BstNSI	RCATGY	2 cut(s)	142, 241
BstSCI	CCNGG	2 cut(s)	448, 626
BstV1I	GCAGC	1 cut(s)	256
BstX2I	RGATCY	1 cut(s)	311
BstYI	RGATCY	1 cut(s)	311
BsuRI	GGCC	1 cut(s)	608
BtrI	CACGTC	1 cut(s)	96
BtsCI	GGATG	1 cut(s)	278
BtsIMutI	CAGTG	2 cut(s)	87, 394
Cac8I	GCNNGC	1 cut(s)	239
CciI	TCATGA	1 cut(s)	66
CseI	GACGC	1 cut(s)	43
Csp6I	GTAC	1 cut(s)	385
CspCI	CAANNNNNGTGG	2 cut(s)	370, 405
CviAII	CATG	3 cut(s)	67, 139, 238
CviJI	RGCY	7 cut(s)	90, 448, 472, 496, 608, 618, 680
CviKI_1	RGCY	7 cut(s)	90, 448, 472, 496, 608, 618, 680
CviQI	GTAC	1 cut(s)	385
DdeI	CTNAG	2 cut(s)	150, 558
DpnI	GATC	1 cut(s)	313
DpnII	GATC	1 cut(s)	311
DraI	TTTAAA	1 cut(s)	652
DrdI	GACNNNNNNGTC	1 cut(s)	76
DseDI	GACNNNNNNGTC	1 cut(s)	76
EaeI	YGGCCR	1 cut(s)	606
Eam1104I	CTCTTC	1 cut(s)	450
EarI	CTCTTC	1 cut(s)	450
Eco130I	CCWWGG	1 cut(s)	477
Eco32I	GATATC	2 cut(s)	307, 546
EcoRII	CCWGG	2 cut(s)	448, 626
EcoRV	GATATC	2 cut(s)	307, 546
EcoT14I	CCWWGG	1 cut(s)	477
ErhI	CCWWGG	1 cut(s)	477
FaeI	CATG	3 cut(s)	70, 142, 241
FatI	CATG	3 cut(s)	66, 138, 237
FauNDI	CATATG	1 cut(s)	691
Fnu4HI	GCNGC	1 cut(s)	270
FokI	GGATG	1 cut(s)	265
Fsp4HI	GCNGC	1 cut(s)	270
GluI	GCNGC	1 cut(s)	270
GsaI	CCCAGC	1 cut(s)	114
HaeIII	GGCC	1 cut(s)	608
HapII	CCGG	2 cut(s)	225, 609
HgaI	GACGC	1 cut(s)	43
Hin1II	CATG	3 cut(s)	70, 142, 241
HindIII	AAGCTT	1 cut(s)	470
HinfI	GANTC	2 cut(s)	358, 562
HpaII	CCGG	2 cut(s)	225, 609
HphI	GGTGA	3 cut(s)	22, 454, 464
Hpy166II	GTNNAC	1 cut(s)	379
Hpy188I	TCNGA	5 cut(s)	121, 196, 311, 433, 561
Hpy188III	TCNNGA	5 cut(s)	31, 67, 157, 302, 362
Hpy8I	GTNNAC	1 cut(s)	379
Hpy99I	CGWCG	3 cut(s)	37, 248, 269
HpyAV	CCTTC	6 cut(s)	66, 271, 350, 434, 454, 606
HpyCH4III	ACNGT	1 cut(s)	389
HpyCH4IV	ACGT	1 cut(s)	95
HpyCH4V	TGCA	2 cut(s)	142, 185
HpyF10VI	GCNNNNNNNGC	2 cut(s)	266, 593
HpyF3I	CTNAG	2 cut(s)	150, 558
HpySE526I	ACGT	1 cut(s)	95
Hsp92II	CATG	3 cut(s)	70, 142, 241
Kzo9I	GATC	1 cut(s)	311
LmnI	GCTCC	1 cut(s)	615
Lsp1109I	GCAGC	1 cut(s)	256
MaeII	ACGT	1 cut(s)	95
MaeIII	GTNAC	1 cut(s)	214
MalI	GATC	1 cut(s)	313
MboI	GATC	1 cut(s)	311
MboII	GAAGA	1 cut(s)	467
MflI	RGATCY	1 cut(s)	311
MluCI	AATT	4 cut(s)	160, 190, 371, 631
MlyI	GAGTC	1 cut(s)	556
MmeI	TCCRAC	1 cut(s)	70
MnlI	CCTC	8 cut(s)	63, 122, 183, 358, 386, 432, 519, 608
MseI	TTAA	1 cut(s)	651
MspA1I	CMGCKG	1 cut(s)	587
MspI	CCGG	2 cut(s)	225, 609
MspR9I	CCNGG	2 cut(s)	450, 628
MvaI	CCWGG	2 cut(s)	450, 628
MwoI	GCNNNNNNNGC	2 cut(s)	266, 593
NdeI	CATATG	1 cut(s)	691
NdeII	GATC	1 cut(s)	311
NlaIII	CATG	3 cut(s)	70, 142, 241
NlaIV	GGNNCC	1 cut(s)	592
NspI	RCATGY	2 cut(s)	142, 241
PaeI	GCATGC	1 cut(s)	241
PagI	TCATGA	1 cut(s)	66
PcsI	WCGNNNNNNNCGW	1 cut(s)	29
PfeI	GAWTC	1 cut(s)	358
PflMI	CCANNNNNTGG	1 cut(s)	633
PkrI	GCNGC	1 cut(s)	271
PleI	GAGTC	1 cut(s)	556
PpsI	GAGTC	1 cut(s)	556
Psp6I	CCWGG	2 cut(s)	448, 626
PspFI	CCCAGC	1 cut(s)	110
PspGI	CCWGG	2 cut(s)	448, 626
PspN4I	GGNNCC	1 cut(s)	592
PsrI	GAACNNNNNNTAC	2 cut(s)	137, 169
PsuI	RGATCY	1 cut(s)	311
RsaI	GTAC	1 cut(s)	386
RsaNI	GTAC	1 cut(s)	385
SaqAI	TTAA	1 cut(s)	651
SatI	GCNGC	1 cut(s)	270
Sau3AI	GATC	1 cut(s)	311
SchI	GAGTC	1 cut(s)	556
ScrFI	CCNGG	2 cut(s)	450, 628
SetI	ASST	9 cut(s)	55, 92, 98, 369, 426, 454, 474, 498, 620
SmlI	CTYRAG	2 cut(s)	362, 674
SmoI	CTYRAG	2 cut(s)	362, 674
SphI	GCATGC	1 cut(s)	241
Sse9I	AATT	4 cut(s)	160, 190, 371, 631
SsiI	CCGC	2 cut(s)	585, 594
StyD4I	CCNGG	2 cut(s)	448, 626
StyI	CCWWGG	1 cut(s)	477
TaaI	ACNGT	1 cut(s)	389
TaiI	ACGT	1 cut(s)	98
TaqI	TCGA	2 cut(s)	23, 32
TasI	AATT	4 cut(s)	160, 190, 371, 631
TatI	WGTACW	1 cut(s)	384
TfiI	GAWTC	1 cut(s)	358
Tru1I	TTAA	1 cut(s)	651
Tru9I	TTAA	1 cut(s)	651
TscAI	CASTG	2 cut(s)	87, 394
TseI	GCWGC	1 cut(s)	269
TspDTI	ATGAA	3 cut(s)	55, 332, 541
TspRI	CASTG	2 cut(s)	87, 394
Van91I	CCANNNNNTGG	1 cut(s)	633
XapI	RAATTY	2 cut(s)	160, 190
XceI	RCATGY	2 cut(s)	142, 241

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.