Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003365

Physical Location & Seq

Genomic Coordinates Forward (+)

31980146 .. 31983378

Gene Structure 3233 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1265600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 795 bp

ATGTCAACGACATACATTTTCCCACAACTTTCTTCTCTTTTCGTCACATCAATGTCAGTGATTAGCTTCACAATATTAGCCGTGTATGGGTTCTTGGAGCTAAGAGAAAAGCACTTACACTATTCCAAGTTCTGGAATTCCGGTTCTAAAACCTTTGCTGTAAAGGAAATCAAACTATCTAGTAGAACAGGCATGCTGATAACTTATACTCCGGCATTTCTTGCAGCTGTGGCATCCTTTGTGATTTATCCTCAAGAGGATATCAGAATGCTGTTGTTGTCTTCGGCTCTGACAATTCACTTTTTGAAAAGGATTTTAGAGGTTCTTTTTGTTCACAAGTACAGCGGAGGAATGATCCTTGATAGTGCTGTTATCATCTCTCTTAGTTATGGTGTGTGTACTGCAAGCGTGACTTATGCTCAATCCTTAACGAAAGGGCTTCCAGAGCCATCAATCGATTTGAAGTACCTTGGCTGTCTGCTGTTTATACTGGGGATTTCTGGCAATTTCTACCATCACTGCCTTCTTTCGAGAATGAGGAGGTTACAGGGTGACAAGGAATATAAGATTCCCAAGGGTGGTTTGTTTGGAATCATTATATGCCCTCACTATCTCTTTGAAATCATAGGATTTGTAGGGCTTGCTCTTATTTCTCAAACTCTCTATGGATTCTCCTTCACCACAGGTTCAGCGTTATACTTGATCGCAAGGAGCTACGTGACGAGAAGATGGTATGTGTCCAAGTTTGAAGATTTTCCCAAAAATGTTAAGGCTCTCATTCCTTATGTTCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0006702 GO:0007154 GO:0007267 GO:0007275 GO:0007548 GO:0008150 GO:0008152 GO:0008202 GO:0008207 GO:0008209 GO:0008406 GO:0008584 GO:0008610 GO:0009058 GO:0009917 GO:0009987 GO:0010817 GO:0012505 GO:0016020 GO:0016229 GO:0016491 GO:0016614 GO:0016616 GO:0016627 GO:0017144 GO:0022414 GO:0023052 GO:0030283 GO:0030539 GO:0030540 GO:0031984 GO:0032501 GO:0032502 GO:0033764 GO:0033765 GO:0034754 GO:0042175 GO:0042180 GO:0042181 GO:0042445 GO:0042446 GO:0042448 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045137 GO:0046546 GO:0046660 GO:0046661 GO:0048513 GO:0048608 GO:0048731 GO:0048806 GO:0048856 GO:0055114 GO:0061370 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00062 ko00140 ko01040 ko01110 ko01212 map00062 map00140 map01040 map01110 map01212

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

265

Amino Acids

29.77

Weight (kDa)

9.45

Isoelectric Point (pI)

48.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	155 - 264	8.2e-25	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003365)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16010
fragaria_vesca	FvH4_2g07871 FvH4_2g07871 FvH4_2g07872 FvH4_2g07900
malus_domestica	MD02G1261000.v1.1 MD02G1265600.v1.1 MD07G1060000.v1.1
prunus_persica	Prupe.2G066100_v2.0.a1 Prupe.2G066100_v2.0.a1 Prupe.2G066300_v2.0.a1 Prupe.2G066400_v2.0.a1 Prupe.2G066400_v2.0.a1
pyrus_communis	pycom02g22310 pycom02g22710
rosa_chinensis	RchiOBHm_Chr6g0262341 RchiOBHm_Chr6g0262351
rosa_laevigata	RLG00000014437 RLG00000014438
rosa_multiflora	Rmu_sc0001544.1_g000021 Rmu_sc0001544.1_g000027 Rmu_sc0028284.1_g000001
rosa_roxburghii	Rroxscaffold_7G00207990
rosa_rugosa	Rorug05G0596300 Rorug05G0596400 Rorug05G0596400
rosa_samantha	Rh6AG116700 Rh6AG117000 Rh6BG110800 Rh6CG108300 Rh6CG108400 Rh6CG108600 Rh6DG098800
rosa_wichuraiana	Rw6G010130 Rw6G010150 Rw6G010160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	132
AciI	CCGC	1 cut(s)	345
AclWI	GGATC	1 cut(s)	349
AcsI	RAATTY	1 cut(s)	136
AfaI	GTAC	3 cut(s)	341, 400, 467
AfiI	CCNNNNNNNGG	3 cut(s)	87, 132, 578
AgsI	TTSAA	4 cut(s)	307, 463, 620, 749
AloI	GAACNNNNNNTCC	2 cut(s)	127, 159
AluBI	AGCT	4 cut(s)	66, 100, 227, 714
AluI	AGCT	4 cut(s)	66, 100, 227, 714
AlwI	GGATC	1 cut(s)	349
ApeKI	GCWGC	1 cut(s)	224
ApoI	RAATTY	1 cut(s)	136
Asp700I	GAANNNNTTC	1 cut(s)	753
AsuHPI	GGTGA	2 cut(s)	563, 670
BbsI	GAAGAC	1 cut(s)	273
BbvI	GCAGC	1 cut(s)	236
BccI	CCATC	3 cut(s)	457, 522, 723
BceAI	ACGGC	1 cut(s)	65
BfaI	CTAG	1 cut(s)	180
BfmI	CTRYAG	1 cut(s)	791
BisI	GCNGC	1 cut(s)	225
BlsI	GCNGC	1 cut(s)	226
BmrI	ACTGGG	1 cut(s)	500
BmsI	GCATC	1 cut(s)	242
BmuI	ACTGGG	1 cut(s)	500
BpiI	GAAGAC	1 cut(s)	273
BpuEI	CTTGAG	1 cut(s)	237
Bsa29I	ATCGAT	1 cut(s)	456
BsaAI	YACGTR	1 cut(s)	718
BsaJI	CCNNGG	2 cut(s)	469, 573
BsaWI	WCCGGW	1 cut(s)	140
BsaXI	ACNNNNNCTCC	2 cut(s)	193, 223
Bsc4I	CCNNNNNNNGG	3 cut(s)	87, 132, 578
Bse1I	ACTGG	1 cut(s)	495
BseCI	ATCGAT	1 cut(s)	456
BseDI	CCNNGG	2 cut(s)	469, 573
BseGI	GGATG	1 cut(s)	233
BseLI	CCNNNNNNNGG	3 cut(s)	87, 132, 578
BseNI	ACTGG	1 cut(s)	495
BseRI	GAGGAG	1 cut(s)	553
BseXI	GCAGC	1 cut(s)	236
BshVI	ATCGAT	1 cut(s)	456
BsiSI	CCGG	2 cut(s)	141, 212
BslI	CCNNNNNNNGG	3 cut(s)	87, 132, 578
BsmI	GAATGC	1 cut(s)	273
Bsp143I	GATC	2 cut(s)	354, 702
BspACI	CCGC	1 cut(s)	345
BspDI	ATCGAT	1 cut(s)	456
BspPI	GGATC	1 cut(s)	349
BsrI	ACTGG	1 cut(s)	495
BssECI	CCNNGG	2 cut(s)	469, 573
BssMI	GATC	2 cut(s)	354, 702
BssT1I	CCWWGG	2 cut(s)	469, 573
BstAPI	GCANNNNNTGC	1 cut(s)	221
BstBAI	YACGTR	1 cut(s)	718
BstC8I	GCNNGC	3 cut(s)	194, 406, 642
BstDEI	CTNAG	2 cut(s)	101, 383
BstF5I	GGATG	1 cut(s)	233
BstKTI	GATC	2 cut(s)	357, 705
BstMBI	GATC	2 cut(s)	354, 702
BstMWI	GCNNNNNNNGC	3 cut(s)	221, 230, 445
BstNSI	RCATGY	1 cut(s)	196
BstSFI	CTRYAG	1 cut(s)	791
BstV1I	GCAGC	1 cut(s)	236
BstV2I	GAAGAC	1 cut(s)	273
Bsu15I	ATCGAT	1 cut(s)	456
BsuTUI	ATCGAT	1 cut(s)	456
BtsCI	GGATG	1 cut(s)	233
BtsI	GCAGTG	1 cut(s)	517
BtsIMutI	CAGTG	2 cut(s)	63, 517
Cac8I	GCNNGC	3 cut(s)	194, 406, 642
ClaI	ATCGAT	1 cut(s)	456
Csp6I	GTAC	3 cut(s)	340, 399, 466
CviAII	CATG	1 cut(s)	193
CviQI	GTAC	3 cut(s)	340, 399, 466
DdeI	CTNAG	2 cut(s)	101, 383
DpnI	GATC	2 cut(s)	356, 704
DpnII	GATC	2 cut(s)	354, 702
Eco130I	CCWWGG	2 cut(s)	469, 573
Eco32I	GATATC	1 cut(s)	262
EcoRI	GAATTC	1 cut(s)	136
EcoRV	GATATC	1 cut(s)	262
EcoT14I	CCWWGG	2 cut(s)	469, 573
ErhI	CCWWGG	2 cut(s)	469, 573
FaeI	CATG	1 cut(s)	196
FalI	AAGNNNNNCTT	2 cut(s)	397, 429
FatI	CATG	1 cut(s)	192
Fnu4HI	GCNGC	1 cut(s)	225
FokI	GGATG	1 cut(s)	220
Fsp4HI	GCNGC	1 cut(s)	225
FspBI	CTAG	1 cut(s)	180
GluI	GCNGC	1 cut(s)	225
HapII	CCGG	2 cut(s)	141, 212
Hin1II	CATG	1 cut(s)	196
HincII	GTYRAC	1 cut(s)	6
HindII	GTYRAC	1 cut(s)	6
HinfI	GANTC	3 cut(s)	568, 591, 669
HpaII	CCGG	2 cut(s)	141, 212
HphI	GGTGA	2 cut(s)	563, 670
Hpy166II	GTNNAC	3 cut(s)	6, 334, 399
Hpy188I	TCNGA	2 cut(s)	266, 291
Hpy188III	TCNNGA	4 cut(s)	133, 254, 443, 531
Hpy8I	GTNNAC	3 cut(s)	6, 334, 399
HpyAV	CCTTC	2 cut(s)	533, 685
HpyCH4IV	ACGT	1 cut(s)	717
HpyCH4V	TGCA	2 cut(s)	224, 404
HpyF10VI	GCNNNNNNNGC	3 cut(s)	221, 230, 445
HpyF3I	CTNAG	2 cut(s)	101, 383
HpySE526I	ACGT	1 cut(s)	717
Hsp92II	CATG	1 cut(s)	196
Kzo9I	GATC	2 cut(s)	354, 702
LmnI	GCTCC	2 cut(s)	97, 711
LpnPI	CCDG	9 cut(s)	118, 154, 174, 225, 456, 476, 486, 533, 669
Lsp1109I	GCAGC	1 cut(s)	236
LweI	GCATC	1 cut(s)	242
MaeI	CTAG	1 cut(s)	180
MaeII	ACGT	1 cut(s)	717
MaeIII	GTNAC	5 cut(s)	43, 409, 543, 551, 718
MalI	GATC	2 cut(s)	356, 704
MboI	GATC	2 cut(s)	354, 702
MboII	GAAGA	4 cut(s)	24, 273, 738, 761
MluCI	AATT	3 cut(s)	136, 294, 505
MnlI	CCTC	7 cut(s)	250, 261, 313, 341, 531, 534, 615
MroXI	GAANNNNTTC	1 cut(s)	753
MseI	TTAA	2 cut(s)	428, 768
MslI	CAYNNNNRTG	1 cut(s)	50
MspA1I	CMGCKG	2 cut(s)	227, 345
MspI	CCGG	2 cut(s)	141, 212
Mva1269I	GAATGC	1 cut(s)	273
MwoI	GCNNNNNNNGC	3 cut(s)	221, 230, 445
NdeII	GATC	2 cut(s)	354, 702
NlaIII	CATG	1 cut(s)	196
NmuCI	GTSAC	4 cut(s)	43, 409, 551, 718
NspI	RCATGY	1 cut(s)	196
PaeI	GCATGC	1 cut(s)	196
PctI	GAATGC	1 cut(s)	273
PdmI	GAANNNNTTC	1 cut(s)	753
PfeI	GAWTC	3 cut(s)	568, 591, 669
PflMI	CCANNNNNTGG	1 cut(s)	132
PkrI	GCNGC	1 cut(s)	226
Ppu21I	YACGTR	1 cut(s)	718
PvuII	CAGCTG	1 cut(s)	227
RsaI	GTAC	3 cut(s)	341, 400, 467
RsaNI	GTAC	3 cut(s)	340, 399, 466
RseI	CAYNNNNRTG	1 cut(s)	50
SaqAI	TTAA	2 cut(s)	428, 768
SatI	GCNGC	1 cut(s)	225
Sau3AI	GATC	2 cut(s)	354, 702
SfaNI	GCATC	1 cut(s)	242
SfcI	CTRYAG	1 cut(s)	791
SmiMI	CAYNNNNRTG	1 cut(s)	50
SmlI	CTYRAG	1 cut(s)	252
SmoI	CTYRAG	1 cut(s)	252
SphI	GCATGC	1 cut(s)	196
Sse9I	AATT	3 cut(s)	136, 294, 505
SsiI	CCGC	1 cut(s)	345
SspI	AATATT	1 cut(s)	75
SspMI	CTAG	1 cut(s)	180
StyI	CCWWGG	2 cut(s)	469, 573
TaiI	ACGT	1 cut(s)	720
TaqI	TCGA	2 cut(s)	456, 530
TasI	AATT	3 cut(s)	136, 294, 505
TatI	WGTACW	2 cut(s)	339, 398
TfiI	GAWTC	3 cut(s)	568, 591, 669
Tru1I	TTAA	2 cut(s)	428, 768
Tru9I	TTAA	2 cut(s)	428, 768
TscAI	CASTG	2 cut(s)	63, 524
TseFI	GTSAC	4 cut(s)	43, 409, 551, 718
TseI	GCWGC	1 cut(s)	224
Tsp45I	GTSAC	4 cut(s)	43, 409, 551, 718
TspRI	CASTG	2 cut(s)	63, 524
Van91I	CCANNNNNTGG	1 cut(s)	132
XapI	RAATTY	1 cut(s)	136
XceI	RCATGY	1 cut(s)	196
XmnI	GAANNNNTTC	1 cut(s)	753
XspI	CTAG	1 cut(s)	180

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.