Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003365

Physical Location & Seq

Genomic Coordinates Reverse (-)

31472370 .. 31473765

Gene Structure 1396 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1261000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 852 bp

ATGGTGATGAGTTGTATGTCGATAATCTCGACACTTATTTTCCCACCACCTCCTTCTATTTTCATGACAGCAATGTCAGTGATTAGCTTCACGTCATTAGCAAATGCTGGGATTTCAGAGATTAGAGGAAAGCACATGCAGTATTCCAAGTTCTGGAATTTCAATAATCCTCAAAATTTTGCACCAAAACTTGAAAAGCAACAAGAAGTAGTAACATTGTCCGGTAGAGCAGGCATGCTCGTTGCTTATACTCCCGCCTTCGTCGCTGCTTTCACATCCTTGCTGTTTTTTTCCCATCACGATGTCAGATCTCTACTGCTCTCTTCAGCTCTAACCCTTCATTTTCTCAAAAGGATTCTTGAGGTTCAATTTGTTCACAAGTATAGTGGAGGAATGGGCGTTGATACAGCAGTTCCGATCTGTCTGAGTTATTTGTTGTCGACTGGAAGCATGATTTATGGTCAATACCTAAGCAGCCAAGTGCTAGGGTTTCCAGAGCCAGCAATTGATTTGAAGAGCGCCGGCATCGTCTTGTTTGTAATAGGAATTTCTGGCAACTTGTACCATCACTACCTTCTTTCGAAGATGAGGTCGAAGCCAGGTGAAGAGAAGGAGTACAAGATTCCCAAGGGTGGTTTGTTTGAACTTGTTATATGCCCTCACTATCTCTTTGAAATCACAACATTTCTTGGGGTTTCTTTCATTTCTCAGACTCTCTACGCTTTCTCCTTCACCGCCGGAACCGCCCTCTACTTGGCCGGAAGGAGCTATGCTACCCGGAATTGGTATCGTTCCAAGTTTAAAGATCATTTTCCCTGTACTTTTAAGGCTCTCATTCCATATGTTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0006702 GO:0007154 GO:0007267 GO:0007275 GO:0007548 GO:0008150 GO:0008152 GO:0008202 GO:0008207 GO:0008209 GO:0008406 GO:0008584 GO:0008610 GO:0009058 GO:0009917 GO:0009987 GO:0010817 GO:0012505 GO:0016020 GO:0016229 GO:0016491 GO:0016614 GO:0016616 GO:0016627 GO:0017144 GO:0022414 GO:0023052 GO:0030283 GO:0030539 GO:0030540 GO:0031984 GO:0032501 GO:0032502 GO:0033764 GO:0033765 GO:0034754 GO:0042175 GO:0042180 GO:0042181 GO:0042445 GO:0042446 GO:0042448 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045137 GO:0046546 GO:0046660 GO:0046661 GO:0048513 GO:0048608 GO:0048731 GO:0048806 GO:0048856 GO:0055114 GO:0061370 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00062 ko00140 ko01040 ko01110 ko01212 map00062 map00140 map01040 map01110 map01212

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

284

Amino Acids

31.65

Weight (kDa)

9.33

Isoelectric Point (pI)

42.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	152 - 283	2.7e-24	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003365)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16010
fragaria_vesca	FvH4_2g07871 FvH4_2g07871 FvH4_2g07872 FvH4_2g07900
malus_domestica	MD02G1261000.v1.1 MD02G1265600.v1.1 MD07G1060000.v1.1
prunus_persica	Prupe.2G066100_v2.0.a1 Prupe.2G066100_v2.0.a1 Prupe.2G066300_v2.0.a1 Prupe.2G066400_v2.0.a1 Prupe.2G066400_v2.0.a1
pyrus_communis	pycom02g22310 pycom02g22710
rosa_chinensis	RchiOBHm_Chr6g0262341 RchiOBHm_Chr6g0262351
rosa_laevigata	RLG00000014437 RLG00000014438
rosa_multiflora	Rmu_sc0001544.1_g000021 Rmu_sc0001544.1_g000027 Rmu_sc0028284.1_g000001
rosa_roxburghii	Rroxscaffold_7G00207990
rosa_rugosa	Rorug05G0596300 Rorug05G0596400 Rorug05G0596400
rosa_samantha	Rh6AG116700 Rh6AG117000 Rh6BG110800 Rh6CG108300 Rh6CG108400 Rh6CG108600 Rh6DG098800
rosa_wichuraiana	Rw6G010130 Rw6G010150 Rw6G010160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	73
AccB7I	CCANNNNNTGG	1 cut(s)	153
AccI	GTMKAC	1 cut(s)	440
AciI	CCGC	3 cut(s)	255, 735, 744
AcoI	YGGCCR	1 cut(s)	756
AcsI	RAATTY	3 cut(s)	157, 175, 546
AcuI	CTGAAG	1 cut(s)	309
AfaI	GTAC	3 cut(s)	563, 617, 820
AfiI	CCNNNNNNNGG	4 cut(s)	153, 632, 754, 783
AgsI	TTSAA	6 cut(s)	163, 194, 368, 514, 644, 674
AjiI	CACGTC	1 cut(s)	93
AjnI	CCWGG	1 cut(s)	598
AluBI	AGCT	3 cut(s)	87, 329, 768
AluI	AGCT	3 cut(s)	87, 329, 768
AoxI	GGCC	1 cut(s)	756
ApeKI	GCWGC	2 cut(s)	266, 474
ApoI	RAATTY	3 cut(s)	157, 175, 546
ArsI	GACNNNNNNTTYG	2 cut(s)	575, 607
AspLEI	GCGC	1 cut(s)	521
AsuC2I	CCSGG	1 cut(s)	778
AsuHPI	GGTGA	3 cut(s)	16, 614, 724
AsuII	TTCGAA	1 cut(s)	581
BaeI	ACNNNNGTAYC	2 cut(s)	396, 429
BbvI	GCAGC	2 cut(s)	253, 486
BccI	CCATC	2 cut(s)	303, 573
BciT130I	CCWGG	1 cut(s)	600
BcnI	CCSGG	1 cut(s)	778
BfaI	CTAG	1 cut(s)	485
BfoI	RGCGCY	1 cut(s)	522
BglII	AGATCT	1 cut(s)	308
BisI	GCNGC	2 cut(s)	267, 475
BlsI	GCNGC	2 cut(s)	268, 476
Bme1390I	CCNGG	2 cut(s)	600, 778
BmgBI	CACGTC	1 cut(s)	93
BmiI	GGNNCC	1 cut(s)	742
BmrFI	CCNGG	2 cut(s)	600, 778
BmsI	GCATC	1 cut(s)	534
Bpu10I	CCTNAGC	1 cut(s)	470
Bpu14I	TTCGAA	1 cut(s)	581
BpuEI	CTTGAG	1 cut(s)	380
BpuMI	CCSGG	1 cut(s)	778
BsaJI	CCNNGG	1 cut(s)	627
BsaWI	WCCGGW	1 cut(s)	221
BsaXI	ACNNNNNCTCC	2 cut(s)	710, 740
Bsc4I	CCNNNNNNNGG	4 cut(s)	153, 632, 754, 783
Bse118I	RCCGGY	1 cut(s)	521
Bse1I	ACTGG	1 cut(s)	448
Bse3DI	GCAATG	1 cut(s)	78
BseBI	CCWGG	1 cut(s)	600
BseDI	CCNNGG	1 cut(s)	627
BseGI	GGATG	1 cut(s)	275
BseLI	CCNNNNNNNGG	4 cut(s)	153, 632, 754, 783
BseMI	GCAATG	1 cut(s)	78
BseMII	CTCAG	2 cut(s)	416, 722
BseNI	ACTGG	1 cut(s)	448
BseXI	GCAGC	2 cut(s)	253, 486
BseYI	CCCAGC	1 cut(s)	107
BshFI	GGCC	1 cut(s)	758
BsiSI	CCGG	5 cut(s)	222, 522, 738, 759, 778
BslI	CCNNNNNNNGG	4 cut(s)	153, 632, 754, 783
BsnI	GGCC	1 cut(s)	758
Bsp119I	TTCGAA	1 cut(s)	581
Bsp143I	GATC	3 cut(s)	308, 417, 805
BspACI	CCGC	3 cut(s)	255, 735, 744
BspANI	GGCC	1 cut(s)	758
BspCNI	CTCAG	2 cut(s)	417, 721
BspHI	TCATGA	1 cut(s)	63
BspLI	GGNNCC	1 cut(s)	742
BspQI	GCTCTTC	1 cut(s)	509
BspT104I	TTCGAA	1 cut(s)	581
BsrDI	GCAATG	1 cut(s)	78
BsrFI	RCCGGY	1 cut(s)	521
BsrI	ACTGG	1 cut(s)	448
BssAI	RCCGGY	1 cut(s)	521
BssECI	CCNNGG	1 cut(s)	627
BssMI	GATC	3 cut(s)	308, 417, 805
BssT1I	CCWWGG	1 cut(s)	627
Bst2UI	CCWGG	1 cut(s)	600
Bst6I	CTCTTC	3 cut(s)	328, 509, 600
BstBI	TTCGAA	1 cut(s)	581
BstC8I	GCNNGC	4 cut(s)	232, 236, 501, 523
BstDEI	CTNAG	3 cut(s)	425, 470, 708
BstF5I	GGATG	1 cut(s)	275
BstH2I	RGCGCY	1 cut(s)	522
BstHHI	GCGC	1 cut(s)	521
BstKTI	GATC	3 cut(s)	311, 420, 808
BstMBI	GATC	3 cut(s)	308, 417, 805
BstMWI	GCNNNNNNNGC	2 cut(s)	263, 743
BstNI	CCWGG	1 cut(s)	600
BstNSI	RCATGY	2 cut(s)	139, 238
BstSCI	CCNGG	2 cut(s)	598, 776
BstV1I	GCAGC	2 cut(s)	253, 486
BstX2I	RGATCY	1 cut(s)	308
BstYI	RGATCY	1 cut(s)	308
BsuRI	GGCC	1 cut(s)	758
BtrI	CACGTC	1 cut(s)	93
BtsCI	GGATG	1 cut(s)	275
BtsIMutI	CAGTG	1 cut(s)	84
Cac8I	GCNNGC	4 cut(s)	232, 236, 501, 523
CciI	TCATGA	1 cut(s)	63
CfoI	GCGC	1 cut(s)	521
Cfr10I	RCCGGY	1 cut(s)	521
Csp6I	GTAC	3 cut(s)	562, 616, 819
CspCI	CAANNNNNGTGG	2 cut(s)	367, 402
CviAII	CATG	4 cut(s)	64, 136, 235, 451
CviJI	RGCY	8 cut(s)	87, 329, 477, 499, 598, 758, 768, 830
CviKI_1	RGCY	8 cut(s)	87, 329, 477, 499, 598, 758, 768, 830
CviQI	GTAC	3 cut(s)	562, 616, 819
DdeI	CTNAG	3 cut(s)	425, 470, 708
DpnI	GATC	3 cut(s)	310, 419, 807
DpnII	GATC	3 cut(s)	308, 417, 805
DraI	TTTAAA	1 cut(s)	802
DrdI	GACNNNNNNGTC	1 cut(s)	73
DseDI	GACNNNNNNGTC	1 cut(s)	73
EaeI	YGGCCR	1 cut(s)	756
Eam1104I	CTCTTC	3 cut(s)	328, 509, 600
EarI	CTCTTC	3 cut(s)	328, 509, 600
Eco130I	CCWWGG	1 cut(s)	627
Eco57I	CTGAAG	1 cut(s)	309
EcoRII	CCWGG	1 cut(s)	598
EcoT14I	CCWWGG	1 cut(s)	627
ErhI	CCWWGG	1 cut(s)	627
FaeI	CATG	4 cut(s)	67, 139, 238, 454
FatI	CATG	4 cut(s)	63, 135, 234, 450
FauI	CCCGC	1 cut(s)	262
FauNDI	CATATG	1 cut(s)	841
FblI	GTMKAC	1 cut(s)	440
Fnu4HI	GCNGC	2 cut(s)	267, 475
FokI	GGATG	1 cut(s)	262
Fsp4HI	GCNGC	2 cut(s)	267, 475
FspBI	CTAG	1 cut(s)	485
GlaI	GCGC	1 cut(s)	520
GluI	GCNGC	2 cut(s)	267, 475
GsaI	CCCAGC	1 cut(s)	111
HaeII	RGCGCY	1 cut(s)	522
HaeIII	GGCC	1 cut(s)	758
HapII	CCGG	5 cut(s)	222, 522, 738, 759, 778
HhaI	GCGC	1 cut(s)	521
Hin1II	CATG	4 cut(s)	67, 139, 238, 454
Hin6I	GCGC	1 cut(s)	519
HinP1I	GCGC	1 cut(s)	519
HincII	GTYRAC	1 cut(s)	441
HindII	GTYRAC	1 cut(s)	441
HinfI	GANTC	3 cut(s)	355, 622, 712
HpaII	CCGG	5 cut(s)	222, 522, 738, 759, 778
HphI	GGTGA	3 cut(s)	16, 614, 724
Hpy166II	GTNNAC	2 cut(s)	376, 441
Hpy188I	TCNGA	5 cut(s)	118, 308, 417, 426, 711
Hpy188III	TCNNGA	6 cut(s)	28, 64, 154, 299, 359, 494
Hpy8I	GTNNAC	2 cut(s)	376, 441
Hpy99I	CGWCG	1 cut(s)	266
HpyAV	CCTTC	7 cut(s)	63, 268, 347, 584, 604, 739, 756
HpyCH4IV	ACGT	1 cut(s)	92
HpyCH4V	TGCA	2 cut(s)	139, 182
HpyF10VI	GCNNNNNNNGC	2 cut(s)	263, 743
HpyF3I	CTNAG	3 cut(s)	425, 470, 708
HpySE526I	ACGT	1 cut(s)	92
Hsp92II	CATG	4 cut(s)	67, 139, 238, 454
HspAI	GCGC	1 cut(s)	519
KroI	GCCGGC	1 cut(s)	521
KroNI	GCCGGC	1 cut(s)	523
Kzo9I	GATC	3 cut(s)	308, 417, 805
LguI	GCTCTTC	1 cut(s)	509
LmnI	GCTCC	1 cut(s)	765
Lsp1109I	GCAGC	2 cut(s)	253, 486
LweI	GCATC	1 cut(s)	534
MaeI	CTAG	1 cut(s)	485
MaeII	ACGT	1 cut(s)	92
MaeIII	GTNAC	1 cut(s)	211
MalI	GATC	3 cut(s)	310, 419, 807
MboI	GATC	3 cut(s)	308, 417, 805
MboII	GAAGA	4 cut(s)	315, 526, 595, 617
MfeI	CAATTG	1 cut(s)	504
MflI	RGATCY	1 cut(s)	308
MluCI	AATT	6 cut(s)	157, 175, 368, 504, 546, 781
MlyI	GAGTC	1 cut(s)	706
MnlI	CCTC	8 cut(s)	60, 119, 180, 355, 383, 582, 669, 758
MroNI	GCCGGC	1 cut(s)	521
MseI	TTAA	2 cut(s)	801, 825
MslI	CAYNNNNRTG	1 cut(s)	300
MspI	CCGG	5 cut(s)	222, 522, 738, 759, 778
MspR9I	CCNGG	2 cut(s)	600, 778
MunI	CAATTG	1 cut(s)	504
MvaI	CCWGG	1 cut(s)	600
MwoI	GCNNNNNNNGC	2 cut(s)	263, 743
NaeI	GCCGGC	1 cut(s)	523
NciI	CCSGG	1 cut(s)	778
NdeI	CATATG	1 cut(s)	841
NdeII	GATC	3 cut(s)	308, 417, 805
NgoMIV	GCCGGC	1 cut(s)	521
NlaIII	CATG	4 cut(s)	67, 139, 238, 454
NlaIV	GGNNCC	1 cut(s)	742
NspI	RCATGY	2 cut(s)	139, 238
NspV	TTCGAA	1 cut(s)	581
PaeI	GCATGC	1 cut(s)	238
PagI	TCATGA	1 cut(s)	63
PciSI	GCTCTTC	1 cut(s)	509
PcsI	WCGNNNNNNNCGW	1 cut(s)	26
PdiI	GCCGGC	1 cut(s)	523
PfeI	GAWTC	2 cut(s)	355, 622
PflMI	CCANNNNNTGG	1 cut(s)	153
PkrI	GCNGC	2 cut(s)	268, 476
PleI	GAGTC	1 cut(s)	706
PpsI	GAGTC	1 cut(s)	706
Psp6I	CCWGG	1 cut(s)	598
PspFI	CCCAGC	1 cut(s)	107
PspGI	CCWGG	1 cut(s)	598
PspN4I	GGNNCC	1 cut(s)	742
PsrI	GAACNNNNNNTAC	2 cut(s)	134, 166
PsuI	RGATCY	1 cut(s)	308
RsaI	GTAC	3 cut(s)	563, 617, 820
RsaNI	GTAC	3 cut(s)	562, 616, 819
RseI	CAYNNNNRTG	1 cut(s)	300
SalI	GTCGAC	1 cut(s)	439
SapI	GCTCTTC	1 cut(s)	509
SaqAI	TTAA	2 cut(s)	801, 825
SatI	GCNGC	2 cut(s)	267, 475
Sau3AI	GATC	3 cut(s)	308, 417, 805
SchI	GAGTC	1 cut(s)	706
ScrFI	CCNGG	2 cut(s)	600, 778
SfaNI	GCATC	1 cut(s)	534
SfuI	TTCGAA	1 cut(s)	581
SmiMI	CAYNNNNRTG	1 cut(s)	300
SmlI	CTYRAG	1 cut(s)	359
SmoI	CTYRAG	1 cut(s)	359
SphI	GCATGC	1 cut(s)	238
Sse9I	AATT	6 cut(s)	157, 175, 368, 504, 546, 781
SsiI	CCGC	3 cut(s)	255, 735, 744
SspMI	CTAG	1 cut(s)	485
StyD4I	CCNGG	2 cut(s)	598, 776
StyI	CCWWGG	1 cut(s)	627
TaiI	ACGT	1 cut(s)	95
TaqI	TCGA	5 cut(s)	20, 29, 440, 581, 593
TasI	AATT	6 cut(s)	157, 175, 368, 504, 546, 781
TatI	WGTACW	2 cut(s)	615, 818
TfiI	GAWTC	2 cut(s)	355, 622
Tru1I	TTAA	2 cut(s)	801, 825
Tru9I	TTAA	2 cut(s)	801, 825
TscAI	CASTG	1 cut(s)	84
TseI	GCWGC	2 cut(s)	266, 474
TspDTI	ATGAA	3 cut(s)	52, 329, 691
TspRI	CASTG	1 cut(s)	84
Van91I	CCANNNNNTGG	1 cut(s)	153
XapI	RAATTY	3 cut(s)	157, 175, 546
XceI	RCATGY	2 cut(s)	139, 238
XmiI	GTMKAC	1 cut(s)	440
XspI	CTAG	1 cut(s)	485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.