Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0003365

Physical Location & Seq

Genomic Coordinates Forward (+)

12414847 .. 12416575

Gene Structure 1729 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG108300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 849 bp

ATGGCGTGGCCAATCATATTTGTTTTTCCACCACCAGCTTCTGTGTTCGTCACAGCCATGTCATTGATCAGCTTCGCAGGGTTAGCTAATGCCGGGTTCTCGGAGGTAAGAGGAAAACACCTCCAGTATTCCAAGTTCCGGATCAATAATAGCAATCAATCCGATTCTCAAAAATCAAAGCAAATTTACATATCTGGTAGAACAGGCATGCAAATTGCTTATACTCCCGCATTTCTTGCTGGTCTCACATCCTTGGTGTTCTTTCCCCATAACGATGATATCAGATATCTATTGCTTTCTTTAGCTCTCACCATTCATTTCCTCAAACGACTTTTGGAGGTTCAATTTGTCCACAAGTACAGTGGAGGGGGGACGCTTGATACTGTGATTCCTATCTCTCTCAGTTACTTCATGTCTACTGTGACTGCGATTTACGCTCAACACCTTACTATAACTCAAGGGCTACCGGAGCCACCAATTGATTTGAAGTATACTGGAATATTATTGTTTCTAATTGGGATATCTGGCAATTTCTACCATCACTACCTACTTTCCCGATTGAGATCATCATCCTCTTCATCTCCGTCAAACTCAAAAAGCAATGACGATAAGGATTATAAGATTCCAAGGGGTGGAATGTTTGAGCTAGTGGTATGCCCTCACTATCTCTTTGAAATCATAGGATTTGTGGGGTTCTCTTTTATTTCTCAGACCTTGTATGCATTCGCCTTCATGGCCGGAACAGCATTCTATTTGAGTGGTAGGAGCTATGCTACTAGGAGATGGTATCTTTCTAAGTTTAAACATTTTCCCCAAAATGTCAAGGCTCTCATTCCATATCTTTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003865 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0006702 GO:0007154 GO:0007267 GO:0007275 GO:0007548 GO:0008150 GO:0008152 GO:0008202 GO:0008207 GO:0008209 GO:0008406 GO:0008584 GO:0008610 GO:0009058 GO:0009917 GO:0009987 GO:0010817 GO:0012505 GO:0016020 GO:0016229 GO:0016491 GO:0016614 GO:0016616 GO:0016627 GO:0017144 GO:0022414 GO:0023052 GO:0030283 GO:0030539 GO:0030540 GO:0031984 GO:0032501 GO:0032502 GO:0033764 GO:0033765 GO:0034754 GO:0042175 GO:0042180 GO:0042181 GO:0042445 GO:0042446 GO:0042448 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045137 GO:0046546 GO:0046660 GO:0046661 GO:0048513 GO:0048608 GO:0048731 GO:0048806 GO:0048856 GO:0055114 GO:0061370 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00062 ko00140 ko01040 ko01110 ko01212 map00062 map00140 map01040 map01110 map01212

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

282

Amino Acids

31.77

Weight (kDa)

9.62

Isoelectric Point (pI)

47.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	162 - 282	4.5e-25	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003365)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16010
fragaria_vesca	FvH4_2g07871 FvH4_2g07871 FvH4_2g07872 FvH4_2g07900
malus_domestica	MD02G1261000.v1.1 MD02G1265600.v1.1 MD07G1060000.v1.1
prunus_persica	Prupe.2G066100_v2.0.a1 Prupe.2G066100_v2.0.a1 Prupe.2G066300_v2.0.a1 Prupe.2G066400_v2.0.a1 Prupe.2G066400_v2.0.a1
pyrus_communis	pycom02g22310 pycom02g22710
rosa_chinensis	RchiOBHm_Chr6g0262341 RchiOBHm_Chr6g0262351
rosa_laevigata	RLG00000014437 RLG00000014438
rosa_multiflora	Rmu_sc0001544.1_g000021 Rmu_sc0001544.1_g000027 Rmu_sc0028284.1_g000001
rosa_roxburghii	Rroxscaffold_7G00207990
rosa_rugosa	Rorug05G0596300 Rorug05G0596400 Rorug05G0596400
rosa_samantha	Rh6AG116700 Rh6AG117000 Rh6BG110800 Rh6CG108300 Rh6CG108400 Rh6CG108600 Rh6DG098800
rosa_wichuraiana	Rw6G010130 Rw6G010150 Rw6G010160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	618
AccB7I	CCANNNNNTGG	1 cut(s)	632
AccI	GTMKAC	2 cut(s)	416, 491
AccIII	TCCGGA	1 cut(s)	138
AciI	CCGC	1 cut(s)	228
AclWI	GGATC	1 cut(s)	149
AcoI	YGGCCR	2 cut(s)	8, 735
AcsI	RAATTY	1 cut(s)	183
AfaI	GTAC	1 cut(s)	359
AfiI	CCNNNNNNNGG	2 cut(s)	138, 632
AgsI	TTSAA	3 cut(s)	344, 487, 674
AluBI	AGCT	6 cut(s)	38, 72, 86, 305, 646, 768
AluI	AGCT	6 cut(s)	38, 72, 86, 305, 646, 768
Alw26I	GTCTC	1 cut(s)	248
AlwI	GGATC	1 cut(s)	149
AlwNI	CAGNNNCTG	1 cut(s)	41
Aor13HI	TCCGGA	1 cut(s)	138
AoxI	GGCC	2 cut(s)	8, 735
ApoI	RAATTY	1 cut(s)	183
AsuC2I	CCSGG	1 cut(s)	94
AsuHPI	GGTGA	1 cut(s)	301
BalI	TGGCCA	1 cut(s)	10
BccI	CCATC	2 cut(s)	546, 777
BclI	TGATCA	1 cut(s)	66
BcnI	CCSGG	1 cut(s)	94
BcoDI	GTCTC	1 cut(s)	248
BfaI	CTAG	2 cut(s)	647, 777
BglI	GCCNNNNNGGC	1 cut(s)	734
Bme1390I	CCNGG	1 cut(s)	94
BmiI	GGNNCC	1 cut(s)	471
BmrFI	CCNGG	1 cut(s)	94
BpmI	CTGGAG	1 cut(s)	107
BpuEI	CTTGAG	1 cut(s)	441
BpuMI	CCSGG	1 cut(s)	94
BsaBI	GATNNNNATC	3 cut(s)	392, 562, 568
BsaI	GGTCTC	1 cut(s)	248
BsaJI	CCNNGG	2 cut(s)	252, 626
BsaWI	WCCGGW	2 cut(s)	138, 466
Bsc4I	CCNNNNNNNGG	2 cut(s)	138, 632
Bse1I	ACTGG	2 cut(s)	124, 499
Bse3DI	GCAATG	1 cut(s)	607
Bse8I	GATNNNNATC	3 cut(s)	392, 562, 568
BseAI	TCCGGA	1 cut(s)	138
BseDI	CCNNGG	2 cut(s)	252, 626
BseGI	GGATG	2 cut(s)	248, 569
BseJI	GATNNNNATC	3 cut(s)	392, 562, 568
BseLI	CCNNNNNNNGG	2 cut(s)	138, 632
BseMI	GCAATG	1 cut(s)	607
BseMII	CTCAG	2 cut(s)	415, 722
BseNI	ACTGG	2 cut(s)	124, 499
BshFI	GGCC	2 cut(s)	10, 737
BsiSI	CCGG	4 cut(s)	93, 139, 467, 738
BslFI	GGGAC	1 cut(s)	385
BslI	CCNNNNNNNGG	2 cut(s)	138, 632
BsmAI	GTCTC	1 cut(s)	248
BsmFI	GGGAC	1 cut(s)	385
BsmI	GAATGC	2 cut(s)	722, 746
BsnI	GGCC	2 cut(s)	10, 737
Bso31I	GGTCTC	1 cut(s)	248
Bsp13I	TCCGGA	1 cut(s)	138
Bsp143I	GATC	3 cut(s)	66, 141, 563
BspACI	CCGC	1 cut(s)	228
BspANI	GGCC	2 cut(s)	10, 737
BspCNI	CTCAG	2 cut(s)	414, 721
BspEI	TCCGGA	1 cut(s)	138
BspLI	GGNNCC	1 cut(s)	471
BspPI	GGATC	1 cut(s)	149
BspTNI	GGTCTC	1 cut(s)	248
BsrDI	GCAATG	1 cut(s)	607
BsrI	ACTGG	2 cut(s)	124, 499
BssECI	CCNNGG	2 cut(s)	252, 626
BssMI	GATC	3 cut(s)	66, 141, 563
BssNAI	GTATAC	1 cut(s)	492
BssT1I	CCWWGG	2 cut(s)	252, 626
Bst1107I	GTATAC	1 cut(s)	492
Bst4CI	ACNGT	3 cut(s)	362, 385, 421
Bst6I	CTCTTC	1 cut(s)	580
BstAPI	GCANNNNNTGC	1 cut(s)	236
BstC8I	GCNNGC	1 cut(s)	209
BstDEI	CTNAG	3 cut(s)	401, 708, 795
BstF5I	GGATG	2 cut(s)	248, 569
BstKTI	GATC	3 cut(s)	69, 144, 566
BstMAI	GTCTC	1 cut(s)	248
BstMBI	GATC	3 cut(s)	66, 141, 563
BstMWI	GCNNNNNNNGC	6 cut(s)	83, 236, 434, 469, 734, 743
BstNSI	RCATGY	1 cut(s)	211
BstSCI	CCNGG	1 cut(s)	92
BstZ17I	GTATAC	1 cut(s)	492
BsuRI	GGCC	2 cut(s)	10, 737
BtsCI	GGATG	2 cut(s)	248, 569
BtsIMutI	CAGTG	1 cut(s)	367
Cac8I	GCNNGC	1 cut(s)	209
CaiI	CAGNNNCTG	1 cut(s)	41
CseI	GACGC	1 cut(s)	382
Csp6I	GTAC	1 cut(s)	358
CspCI	CAANNNNNGTGG	2 cut(s)	343, 378
CviAII	CATG	4 cut(s)	58, 208, 412, 733
CviQI	GTAC	1 cut(s)	358
DdeI	CTNAG	3 cut(s)	401, 708, 795
DpnI	GATC	3 cut(s)	68, 143, 565
DpnII	GATC	3 cut(s)	66, 141, 563
DraI	TTTAAA	1 cut(s)	802
EaeI	YGGCCR	2 cut(s)	8, 735
Eam1104I	CTCTTC	1 cut(s)	580
EarI	CTCTTC	1 cut(s)	580
Eco130I	CCWWGG	2 cut(s)	252, 626
Eco31I	GGTCTC	1 cut(s)	248
Eco32I	GATATC	3 cut(s)	280, 287, 522
EcoRV	GATATC	3 cut(s)	280, 287, 522
EcoT14I	CCWWGG	2 cut(s)	252, 626
EcoT22I	ATGCAT	1 cut(s)	724
ErhI	CCWWGG	2 cut(s)	252, 626
FaeI	CATG	4 cut(s)	61, 211, 415, 736
FaqI	GGGAC	1 cut(s)	385
FatI	CATG	4 cut(s)	57, 207, 411, 732
FauI	CCCGC	1 cut(s)	235
FbaI	TGATCA	1 cut(s)	66
FblI	GTMKAC	2 cut(s)	416, 491
FokI	GGATG	2 cut(s)	235, 556
FspBI	CTAG	2 cut(s)	647, 777
GsuI	CTGGAG	1 cut(s)	107
HaeIII	GGCC	2 cut(s)	10, 737
HapII	CCGG	4 cut(s)	93, 139, 467, 738
HgaI	GACGC	1 cut(s)	382
Hin1II	CATG	4 cut(s)	61, 211, 415, 736
HinfI	GANTC	3 cut(s)	164, 388, 622
HpaII	CCGG	4 cut(s)	93, 139, 467, 738
HphI	GGTGA	1 cut(s)	301
Hpy166II	GTNNAC	3 cut(s)	352, 417, 492
Hpy188I	TCNGA	4 cut(s)	103, 163, 284, 711
Hpy188III	TCNNGA	2 cut(s)	139, 555
Hpy8I	GTNNAC	3 cut(s)	352, 417, 492
HpyAV	CCTTC	1 cut(s)	739
HpyCH4III	ACNGT	3 cut(s)	362, 385, 421
HpyCH4V	TGCA	2 cut(s)	211, 722
HpyF10VI	GCNNNNNNNGC	6 cut(s)	83, 236, 434, 469, 734, 743
HpyF3I	CTNAG	3 cut(s)	401, 708, 795
Hsp92II	CATG	4 cut(s)	61, 211, 415, 736
Kpn2I	TCCGGA	1 cut(s)	138
Ksp22I	TGATCA	1 cut(s)	66
Kzo9I	GATC	3 cut(s)	66, 141, 563
LmnI	GCTCC	2 cut(s)	469, 765
MaeI	CTAG	2 cut(s)	647, 777
MaeIII	GTNAC	3 cut(s)	49, 404, 421
MalI	GATC	3 cut(s)	68, 143, 565
MboI	GATC	3 cut(s)	66, 141, 563
MboII	GAAGA	1 cut(s)	567
MfeI	CAATTG	1 cut(s)	477
MlsI	TGGCCA	1 cut(s)	10
MluCI	AATT	6 cut(s)	183, 213, 344, 477, 513, 529
MluNI	TGGCCA	1 cut(s)	10
MnlI	CCTC	8 cut(s)	97, 104, 131, 331, 332, 359, 583, 669
Mox20I	TGGCCA	1 cut(s)	10
Mph1103I	ATGCAT	1 cut(s)	724
MroI	TCCGGA	1 cut(s)	138
MscI	TGGCCA	1 cut(s)	10
MseI	TTAA	1 cut(s)	801
MslI	CAYNNNNRTG	2 cut(s)	56, 273
Msp20I	TGGCCA	1 cut(s)	10
MspI	CCGG	4 cut(s)	93, 139, 467, 738
MspR9I	CCNGG	1 cut(s)	94
MssI	GTTTAAAC	1 cut(s)	802
MunI	CAATTG	1 cut(s)	477
Mva1269I	GAATGC	2 cut(s)	722, 746
MwoI	GCNNNNNNNGC	6 cut(s)	83, 236, 434, 469, 734, 743
NciI	CCSGG	1 cut(s)	94
NdeII	GATC	3 cut(s)	66, 141, 563
NlaIII	CATG	4 cut(s)	61, 211, 415, 736
NlaIV	GGNNCC	1 cut(s)	471
NmuCI	GTSAC	2 cut(s)	49, 421
NsiI	ATGCAT	1 cut(s)	724
NspI	RCATGY	1 cut(s)	211
PaeI	GCATGC	1 cut(s)	211
PctI	GAATGC	2 cut(s)	722, 746
PfeI	GAWTC	3 cut(s)	164, 388, 622
PflMI	CCANNNNNTGG	1 cut(s)	632
PmeI	GTTTAAAC	1 cut(s)	802
PsiI	TTATAA	1 cut(s)	618
PspN4I	GGNNCC	1 cut(s)	471
PsrI	GAACNNNNNNTAC	2 cut(s)	119, 151
PstNI	CAGNNNCTG	1 cut(s)	41
RsaI	GTAC	1 cut(s)	359
RsaNI	GTAC	1 cut(s)	358
RseI	CAYNNNNRTG	2 cut(s)	56, 273
SaqAI	TTAA	1 cut(s)	801
Sau3AI	GATC	3 cut(s)	66, 141, 563
ScrFI	CCNGG	1 cut(s)	94
SmiMI	CAYNNNNRTG	2 cut(s)	56, 273
SmlI	CTYRAG	1 cut(s)	456
SmoI	CTYRAG	1 cut(s)	456
SphI	GCATGC	1 cut(s)	211
Sse9I	AATT	6 cut(s)	183, 213, 344, 477, 513, 529
SsiI	CCGC	1 cut(s)	228
SspI	AATATT	1 cut(s)	501
SspMI	CTAG	2 cut(s)	647, 777
StyD4I	CCNGG	1 cut(s)	92
StyI	CCWWGG	2 cut(s)	252, 626
TaaI	ACNGT	3 cut(s)	362, 385, 421
TasI	AATT	6 cut(s)	183, 213, 344, 477, 513, 529
TatI	WGTACW	1 cut(s)	357
TfiI	GAWTC	3 cut(s)	164, 388, 622
Tru1I	TTAA	1 cut(s)	801
Tru9I	TTAA	1 cut(s)	801
TscAI	CASTG	1 cut(s)	367
TseFI	GTSAC	2 cut(s)	49, 421
Tsp45I	GTSAC	2 cut(s)	49, 421
TspDTI	ATGAA	4 cut(s)	305, 400, 567, 721
TspGWI	ACGGA	1 cut(s)	573
TspRI	CASTG	1 cut(s)	367
Van91I	CCANNNNNTGG	1 cut(s)	632
XapI	RAATTY	1 cut(s)	183
XceI	RCATGY	1 cut(s)	211
XcmI	CCANNNNNNNNNTGG	1 cut(s)	359
XmiI	GTMKAC	2 cut(s)	416, 491
XspI	CTAG	2 cut(s)	647, 777
Zsp2I	ATGCAT	1 cut(s)	724

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.