AT5G18630

lipase class 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002948
Physical Location & Seq
Forward (+)
6202536 .. 6205737
3202 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18630.3

Sequence Viewer

Length: 1077 bp
ATGGGACAAAAGAGATTGTTGTTCTTGCTGGCAGTTTTTACTTTTCTTGTTTCATCTTCATATGGTAGAGGTGTTCTCAAGTTGAAGAGCGATGATGATCGACCTGTTTACAACCATACGCTTGCCGTCACGCTTGTGGAGTATGCCTCTGCGGTCTATGAGTCTGATTTGACAAAACTCTTCACTTGGACGTGCGAAAGATGCAATGGTTTGACAAAGGATTTCGAAGTAATAGAGGTCATATTTGACGTTGAACACTGCTTACAGGCGTATGTTGGGGTGGCTAAGGATTTGAATGCTATCATTATTGCATTTCGGGGAACTCAAGAACACAGCATACAAAATTGGGTCTCAGACTTGTTCTGGAAACAGCTCGATCTGAATTACCCTGACATGCCAGATGCAATGGTGCACCATGGCTTTTACAGTGCTTATCACAATACAACTTTACGGCCTGCGGTTTTGGATGCAATAACACGAGTGAAAAAAGTCTACGGGGCGAACATCAACATCATAGTGACCGGTCATTCAATGGGAGGAGCCATGGCTTCCTTTTGTGGTCTAGACCTAGTTGTAAATGAAGGTGAAGAAAACGTACAGGTTATGACATTTGGGCAACCTCGTGTTGGGAATGCCGCTTTTGCATCTTATTACAGTTTGCTTGTGCCCAATACCTTCCGGATCACGCATGACCGTGATATGGTTCCTCATCTGCCTCCTTACTATTACCATTTCCCTCAAAAAACATACCACCACTTCCCAACAGAGGTGTGGGTGAAAGATTTCAGTTTTTCGAATTTTGTTCTTTTTGGTTTGGAGAAAGTTTGTGACAACACTGGTGAAGATCCTACTTGCAGCAGATCGGTAAGGGGAAATAGCATTTCTGACCATTTAAGGTACTTTGGGGTAGAGTTGAAGTGTGAGAGTTGGAGACAGTGCAGCATAGTGATGAATCAAGATATGGAGCGTTACAGCAGGAAAGATTCAAGGGGTAACTTATTCCTCTCGCGGACAGTTCCTCCTAAGGACGTAGTCAAAACAAGTTCTCTATCGAAAACCGGAATCTCTAGTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

358

Amino Acids

40.67

Weight (kDa)

6.49

Isoelectric Point (pI)

28.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 101 - 240 1.6e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 492
AccII CGCG 1 cut(s) 1009
AccIII TCCGGA 1 cut(s) 678
AciI CCGC 4 cut(s) 152, 458, 636, 1009
AclWI GGATC 2 cut(s) 689, 839
AcsI RAATTY 1 cut(s) 796
AfaI GTAC 2 cut(s) 597, 899
AfiI CCNNNNNNNGG 3 cut(s) 626, 700, 766
AgeI ACCGGT 1 cut(s) 521
AgsI TTSAA 6 cut(s) 85, 254, 295, 531, 916, 987
AjiI CACGTC 1 cut(s) 192
AleI CACNNNNGTG 1 cut(s) 134
AluBI AGCT 1 cut(s) 373
AluI AGCT 1 cut(s) 373
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 2 cut(s) 355, 925
Alw44I GTGCAC 1 cut(s) 410
AlwI GGATC 2 cut(s) 689, 839
Aor13HI TCCGGA 1 cut(s) 678
AoxI GGCC 1 cut(s) 452
ApaLI GTGCAC 1 cut(s) 410
ApeKI GCWGC 2 cut(s) 855, 939
ApoI RAATTY 1 cut(s) 796
AsiGI ACCGGT 1 cut(s) 521
Asp700I GAANNNNTTC 1 cut(s) 782
AsuHPI GGTGA 3 cut(s) 596, 787, 851
AsuII TTCGAA 2 cut(s) 225, 794
AxyI CCTNAGG 1 cut(s) 1023
BaeGI GKGCMC 2 cut(s) 414, 669
BarI GAAGNNNNNNTAC 2 cut(s) 579, 611
BauI CACGAG 2 cut(s) 477, 621
Bbv12I GWGCWC 1 cut(s) 414
BbvI GCAGC 2 cut(s) 867, 951
BceAI ACGGC 2 cut(s) 110, 467
BcoDI GTCTC 2 cut(s) 355, 925
BfaI CTAG 3 cut(s) 563, 569, 1068
BfmI CTRYAG 1 cut(s) 1073
BisI GCNGC 3 cut(s) 636, 856, 940
BlsI GCNGC 3 cut(s) 637, 857, 941
BmgBI CACGTC 1 cut(s) 192
BmiI GGNNCC 2 cut(s) 541, 705
BmsI GCATC 4 cut(s) 191, 391, 457, 653
BplI GAGNNNNNCTC 4 cut(s) 60, 92, 131, 163
Bpu10I CCTNAGC 1 cut(s) 285
Bpu14I TTCGAA 2 cut(s) 225, 794
BpuEI CTTGAG 2 cut(s) 62, 309
BsaBI GATNNNNATC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 355
BsaJI CCNNGG 2 cut(s) 415, 543
BsaWI WCCGGW 3 cut(s) 521, 678, 1058
BsaXI ACNNNNNCTCC 2 cut(s) 1003, 1033
Bsc4I CCNNNNNNNGG 3 cut(s) 626, 700, 766
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 1 cut(s) 841
Bse21I CCTNAGG 1 cut(s) 1023
Bse3DI GCAATG 2 cut(s) 211, 411
Bse8I GATNNNNATC 1 cut(s) 96
BseAI TCCGGA 1 cut(s) 678
BseDI CCNNGG 2 cut(s) 415, 543
BseGI GGATG 1 cut(s) 472
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 3 cut(s) 626, 700, 766
BseMI GCAATG 2 cut(s) 211, 411
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 841
BseRI GAGGAG 1 cut(s) 552
BseSI GKGCMC 2 cut(s) 414, 669
BseXI GCAGC 2 cut(s) 867, 951
BsgI GTGCAG 1 cut(s) 958
Bsh1236I CGCG 1 cut(s) 1009
BshFI GGCC 1 cut(s) 454
BshTI ACCGGT 1 cut(s) 521
BsiHKAI GWGCWC 1 cut(s) 414
BsiSI CCGG 3 cut(s) 522, 679, 1059
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 3 cut(s) 626, 700, 766
BsmAI GTCTC 2 cut(s) 355, 925
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 2 cut(s) 301, 637
BsnI GGCC 1 cut(s) 454
Bso31I GGTCTC 1 cut(s) 355
Bsp119I TTCGAA 2 cut(s) 225, 794
Bsp1286I GDGCHC 2 cut(s) 414, 669
Bsp13I TCCGGA 1 cut(s) 678
Bsp143I GATC 5 cut(s) 97, 376, 681, 844, 860
Bsp19I CCATGG 2 cut(s) 415, 543
BspACI CCGC 4 cut(s) 152, 458, 636, 1009
BspANI GGCC 1 cut(s) 454
BspCNI CTCAG 1 cut(s) 365
BspEI TCCGGA 1 cut(s) 678
BspFNI CGCG 1 cut(s) 1009
BspLI GGNNCC 2 cut(s) 541, 705
BspPI GGATC 2 cut(s) 689, 839
BspQI GCTCTTC 1 cut(s) 80
BspT104I TTCGAA 2 cut(s) 225, 794
BspTNI GGTCTC 1 cut(s) 355
BsrDI GCAATG 2 cut(s) 211, 411
BsrFI RCCGGY 1 cut(s) 521
BsrI ACTGG 1 cut(s) 841
BssAI RCCGGY 1 cut(s) 521
BssECI CCNNGG 2 cut(s) 415, 543
BssMI GATC 5 cut(s) 97, 376, 681, 844, 860
BssSI CACGAG 2 cut(s) 477, 621
BssT1I CCWWGG 2 cut(s) 415, 543
Bst2BI CACGAG 2 cut(s) 477, 621
Bst4CI ACNGT 5 cut(s) 428, 656, 695, 936, 1015
Bst6I CTCTTC 2 cut(s) 80, 185
BstBI TTCGAA 2 cut(s) 225, 794
BstC8I GCNNGC 3 cut(s) 30, 123, 456
BstDEI CTNAG 3 cut(s) 285, 352, 1023
BstDSI CCRYGG 2 cut(s) 415, 543
BstF5I GGATG 1 cut(s) 472
BstFNI CGCG 1 cut(s) 1009
BstKTI GATC 5 cut(s) 100, 379, 684, 847, 863
BstMAI GTCTC 2 cut(s) 355, 925
BstMBI GATC 5 cut(s) 97, 376, 681, 844, 860
BstMWI GCNNNNNNNGC 2 cut(s) 201, 641
BstNSI RCATGY 1 cut(s) 397
BstSFI CTRYAG 1 cut(s) 1073
BstSLI GKGCMC 2 cut(s) 414, 669
BstUI CGCG 1 cut(s) 1009
BstV1I GCAGC 2 cut(s) 867, 951
BstX2I RGATCY 1 cut(s) 844
BstYI RGATCY 1 cut(s) 844
Bsu36I CCTNAGG 1 cut(s) 1023
BsuRI GGCC 1 cut(s) 454
BtgI CCRYGG 2 cut(s) 415, 543
BtgZI GCGATG 1 cut(s) 105
BtrI CACGTC 1 cut(s) 192
BtsCI GGATG 1 cut(s) 472
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 4 cut(s) 256, 433, 834, 941
Cac8I GCNNGC 3 cut(s) 30, 123, 456
Cfr10I RCCGGY 1 cut(s) 521
Csp6I GTAC 2 cut(s) 596, 898
CspAI ACCGGT 1 cut(s) 521
CviAII CATG 4 cut(s) 394, 416, 544, 689
CviJI RGCY 6 cut(s) 284, 373, 420, 454, 542, 548
CviKI_1 RGCY 6 cut(s) 284, 373, 420, 454, 542, 548
CviQI GTAC 2 cut(s) 596, 898
DdeI CTNAG 3 cut(s) 285, 352, 1023
DpnI GATC 5 cut(s) 99, 378, 683, 846, 862
DpnII GATC 5 cut(s) 97, 376, 681, 844, 860
Eam1104I CTCTTC 2 cut(s) 80, 185
EarI CTCTTC 2 cut(s) 80, 185
Eco130I CCWWGG 2 cut(s) 415, 543
Eco31I GGTCTC 1 cut(s) 355
Eco81I CCTNAGG 1 cut(s) 1023
EcoT14I CCWWGG 2 cut(s) 415, 543
ErhI CCWWGG 2 cut(s) 415, 543
FaeI CATG 4 cut(s) 397, 419, 547, 692
FaqI GGGAC 1 cut(s) 18
FatI CATG 4 cut(s) 393, 415, 543, 688
FauNDI CATATG 1 cut(s) 61
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 3 cut(s) 636, 856, 940
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 3 cut(s) 636, 856, 940
FspBI CTAG 3 cut(s) 563, 569, 1068
GluI GCNGC 3 cut(s) 636, 856, 940
HaeIII GGCC 1 cut(s) 454
HapII CCGG 3 cut(s) 522, 679, 1059
Hin1II CATG 4 cut(s) 397, 419, 547, 692
HinfI GANTC 4 cut(s) 161, 952, 983, 1062
HpaII CCGG 3 cut(s) 522, 679, 1059
HphI GGTGA 3 cut(s) 596, 787, 851
Hpy166II GTNNAC 3 cut(s) 109, 412, 493
Hpy188I TCNGA 4 cut(s) 166, 355, 381, 886
Hpy188III TCNNGA 5 cut(s) 326, 364, 563, 679, 956
Hpy8I GTNNAC 3 cut(s) 109, 412, 493
HpyAV CCTTC 2 cut(s) 575, 685
HpyCH4III ACNGT 5 cut(s) 428, 656, 695, 936, 1015
HpyCH4IV ACGT 4 cut(s) 191, 249, 594, 1029
HpyCH4V TGCA 8 cut(s) 204, 311, 404, 412, 470, 644, 855, 939
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 641
HpyF3I CTNAG 3 cut(s) 285, 352, 1023
HpySE526I ACGT 4 cut(s) 191, 249, 594, 1029
Hsp92II CATG 4 cut(s) 397, 419, 547, 692
Kpn2I TCCGGA 1 cut(s) 678
Kzo9I GATC 5 cut(s) 97, 376, 681, 844, 860
LguI GCTCTTC 1 cut(s) 80
LmnI GCTCC 2 cut(s) 539, 964
Lsp1109I GCAGC 2 cut(s) 867, 951
LweI GCATC 4 cut(s) 191, 391, 457, 653
MaeI CTAG 3 cut(s) 563, 569, 1068
MaeII ACGT 4 cut(s) 191, 249, 594, 1029
MaeIII GTNAC 5 cut(s) 127, 517, 827, 968, 992
MalI GATC 5 cut(s) 99, 378, 683, 846, 862
MboI GATC 5 cut(s) 97, 376, 681, 844, 860
MboII GAAGA 5 cut(s) 48, 97, 172, 599, 854
MflI RGATCY 1 cut(s) 844
MhlI GDGCHC 2 cut(s) 414, 669
MluCI AATT 3 cut(s) 343, 382, 796
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 908
MroI TCCGGA 1 cut(s) 678
MroXI GAANNNNTTC 1 cut(s) 782
MseI TTAA 1 cut(s) 893
MslI CAYNNNNRTG 4 cut(s) 134, 515, 693, 947
MspI CCGG 3 cut(s) 522, 679, 1059
Mva1269I GAATGC 2 cut(s) 301, 637
MvnI CGCG 1 cut(s) 1009
MwoI GCNNNNNNNGC 2 cut(s) 201, 641
NcoI CCATGG 2 cut(s) 415, 543
NdeI CATATG 1 cut(s) 61
NdeII GATC 5 cut(s) 97, 376, 681, 844, 860
NlaIII CATG 4 cut(s) 397, 419, 547, 692
NlaIV GGNNCC 2 cut(s) 541, 705
NmuCI GTSAC 3 cut(s) 127, 517, 827
NspI RCATGY 1 cut(s) 397
NspV TTCGAA 2 cut(s) 225, 794
OliI CACNNNNGTG 1 cut(s) 134
PciSI GCTCTTC 1 cut(s) 80
PctI GAATGC 2 cut(s) 301, 637
PdmI GAANNNNTTC 1 cut(s) 782
PfeI GAWTC 3 cut(s) 952, 983, 1062
PflFI GACNNNGTC 1 cut(s) 1031
PinAI ACCGGT 1 cut(s) 521
PkrI GCNGC 3 cut(s) 637, 857, 941
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 541, 705
PsrI GAACNNNNNNTAC 4 cut(s) 246, 278, 321, 353
PsuI RGATCY 1 cut(s) 844
PsyI GACNNNGTC 1 cut(s) 1031
RsaI GTAC 2 cut(s) 597, 899
RsaNI GTAC 2 cut(s) 596, 898
RseI CAYNNNNRTG 4 cut(s) 134, 515, 693, 947
SapI GCTCTTC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 893
SatI GCNGC 3 cut(s) 636, 856, 940
Sau3AI GATC 5 cut(s) 97, 376, 681, 844, 860
SchI GAGTC 1 cut(s) 170
SduI GDGCHC 2 cut(s) 414, 669
SfaNI GCATC 4 cut(s) 191, 391, 457, 653
SfcI CTRYAG 1 cut(s) 1073
SfuI TTCGAA 2 cut(s) 225, 794
SmiMI CAYNNNNRTG 4 cut(s) 134, 515, 693, 947
SmlI CTYRAG 2 cut(s) 77, 324
SmoI CTYRAG 2 cut(s) 77, 324
Sse9I AATT 3 cut(s) 343, 382, 796
SsiI CCGC 4 cut(s) 152, 458, 636, 1009
SspMI CTAG 3 cut(s) 563, 569, 1068
StyI CCWWGG 2 cut(s) 415, 543
TaaI ACNGT 5 cut(s) 428, 656, 695, 936, 1015
TaiI ACGT 4 cut(s) 194, 252, 597, 1032
TaqI TCGA 5 cut(s) 100, 225, 375, 794, 1052
TasI AATT 3 cut(s) 343, 382, 796
TauI GCSGC 1 cut(s) 638
TfiI GAWTC 3 cut(s) 952, 983, 1062
Tru1I TTAA 1 cut(s) 893
Tru9I TTAA 1 cut(s) 893
TscAI CASTG 4 cut(s) 263, 433, 841, 941
TseFI GTSAC 3 cut(s) 127, 517, 827
TseI GCWGC 2 cut(s) 855, 939
Tsp45I GTSAC 3 cut(s) 127, 517, 827
TspDTI ATGAA 4 cut(s) 42, 48, 594, 965
TspRI CASTG 4 cut(s) 263, 433, 841, 941
Tth111I GACNNNGTC 1 cut(s) 1031
VneI GTGCAC 1 cut(s) 410
XapI RAATTY 1 cut(s) 796
XbaI TCTAGA 1 cut(s) 562
XceI RCATGY 1 cut(s) 397
XcmI CCANNNNNNNNNTGG 1 cut(s) 768
XmiI GTMKAC 1 cut(s) 492
XmnI GAANNNNTTC 1 cut(s) 782
XspI CTAG 3 cut(s) 563, 569, 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.