Rroxscaffold_2G00120370

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002948
Physical Location & Seq
Reverse (-)
52578192 .. 52582426
4235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00120370.1

Sequence Viewer

Length: 870 bp
ATGGTTGCTGTTGTTGGCACTACTTACTTTGCCCCTTTGCTTCTTCTGCTGGGAGAGGTGTATTTGTCAGATTTGACGGAGCTGTTTTCTTGGACATGCGACAGATGTGATGGCTTGATTAAGGCATTTGTTGGGGTGGCAACAGATCCAAATGCTATTATAATTGCATTTAAAGGGACTCAGGGAAACAGCATACAGAATTGGATCGAAGACTTATTTTGGAAACAACTTGATATAGATTACCCTGGCATGCCTGATGCAATGGTGCACCATGGTTTTTATAATGCTTACCACAACACAACCATACGACCTGGAATTCTCAATGCTGTTGCAAGAGCCAAAGAGTTTTATGGAGATATTGGCATCATAGTGACAGGGCATTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATTTAAGGGTAAATCAAAAGGAGAACAATGTTCAAGTTATGACATTTGGACAACCTCGAATTGGTAATGCAATATTTGCTACTTACTATAGCAAAATTCTGCCACAGACTATACGAGTAACAAATGGAAATGATGTTGTGCCTCATTTGCCTCCATACTACACTTATTTTCCTCAGAAGACATACCACCACTTCCCAACAGAGTTTCGATGTTACAAGTTGTTGAGACGTCTGTGCAGGTCAGTGATAGGGGACAACATTTTAGACCATTTAGCTTATTATGGTGTCGAATTAAAGGACGAGACATGGAGACCGTGCAAAATTGTGATGGGTCCTGGTTTACTGGATCATGGAAGATCAGATATGGAAGGAAATTTTGTTCTGTCCAGAGATCTTGCAACTCCTGTCCTCAAATTGAAAATACAATCTGACGCTGGGGGAAAGCCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

289

Amino Acids

32.42

Weight (kDa)

6.74

Isoelectric Point (pI)

29.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 54 - 192 1.6e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 161, 282
AatII GACGTC 1 cut(s) 649
Acc36I ACCTGC 1 cut(s) 645
AclWI GGATC 3 cut(s) 140, 212, 771
AcsI RAATTY 3 cut(s) 315, 513, 790
AcyI GRCGYC 1 cut(s) 646
AfiI CCNNNNNNNGG 1 cut(s) 479
AgsI TTSAA 3 cut(s) 384, 452, 835
AjnI CCWGG 3 cut(s) 244, 310, 751
AloI GAACNNNNNNTCC 2 cut(s) 780, 812
AluBI AGCT 2 cut(s) 82, 692
AluI AGCT 2 cut(s) 82, 692
Alw21I GWGCWC 2 cut(s) 270, 415
Alw26I GTCTC 3 cut(s) 637, 713, 721
Alw44I GTGCAC 1 cut(s) 266
AlwI GGATC 3 cut(s) 140, 212, 771
ApaLI GTGCAC 1 cut(s) 266
ApoI RAATTY 3 cut(s) 315, 513, 790
AspS9I GGNCC 1 cut(s) 749
AvaII GGWCC 1 cut(s) 749
BaeGI GKGCMC 1 cut(s) 270
BbsI GAAGAC 2 cut(s) 216, 602
Bbv12I GWGCWC 2 cut(s) 270, 415
BccI CCATC 2 cut(s) 104, 739
BciT130I CCWGG 3 cut(s) 246, 312, 753
BcoDI GTCTC 3 cut(s) 637, 713, 721
BfmI CTRYAG 1 cut(s) 505
BfuAI ACCTGC 1 cut(s) 645
BglII AGATCT 1 cut(s) 808
Bme1390I CCNGG 3 cut(s) 246, 312, 753
Bme18I GGWCC 1 cut(s) 749
BmgT120I GGNCC 1 cut(s) 749
BmiI GGNNCC 1 cut(s) 750
BmrFI CCNGG 3 cut(s) 246, 312, 753
BmsI GCATC 2 cut(s) 247, 372
BpiI GAAGAC 2 cut(s) 216, 602
BsaHI GRCGYC 1 cut(s) 646
BsaI GGTCTC 1 cut(s) 721
BsaJI CCNNGG 2 cut(s) 244, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse1I ACTGG 1 cut(s) 765
Bse3DI GCAATG 2 cut(s) 267, 402
BseBI CCWGG 3 cut(s) 246, 312, 753
BseDI CCNNGG 2 cut(s) 244, 271
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMI GCAATG 2 cut(s) 267, 402
BseMII CTCAG 2 cut(s) 194, 605
BseNI ACTGG 1 cut(s) 765
BseSI GKGCMC 1 cut(s) 270
BseYI CCCAGC 2 cut(s) 49, 851
BsgI GTGCAG 1 cut(s) 673
BsiHKAI GWGCWC 2 cut(s) 270, 415
BslFI GGGAC 2 cut(s) 190, 683
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 3 cut(s) 637, 713, 721
BsmBI CGTCTC 1 cut(s) 637
BsmFI GGGAC 2 cut(s) 190, 683
BsmI GAATGC 1 cut(s) 379
Bso31I GGTCTC 1 cut(s) 721
Bsp1286I GDGCHC 2 cut(s) 270, 415
Bsp143I GATC 5 cut(s) 145, 204, 763, 773, 808
Bsp19I CCATGG 1 cut(s) 271
BspCNI CTCAG 2 cut(s) 193, 604
BspLI GGNNCC 1 cut(s) 750
BspMI ACCTGC 1 cut(s) 645
BspPI GGATC 3 cut(s) 140, 212, 771
BspTNI GGTCTC 1 cut(s) 721
BsrDI GCAATG 2 cut(s) 267, 402
BsrI ACTGG 1 cut(s) 765
BssECI CCNNGG 2 cut(s) 244, 271
BssMI GATC 5 cut(s) 145, 204, 763, 773, 808
BssNI GRCGYC 1 cut(s) 646
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 3 cut(s) 246, 312, 753
Bst4CI ACNGT 1 cut(s) 732
BstACI GRCGYC 1 cut(s) 646
BstAPI GCANNNNNTGC 1 cut(s) 494
BstC8I GCNNGC 1 cut(s) 251
BstDEI CTNAG 2 cut(s) 180, 591
BstDSI CCRYGG 1 cut(s) 271
BstKTI GATC 5 cut(s) 148, 207, 766, 776, 811
BstMAI GTCTC 3 cut(s) 637, 713, 721
BstMBI GATC 5 cut(s) 145, 204, 763, 773, 808
BstMWI GCNNNNNNNGC 3 cut(s) 46, 494, 565
BstNI CCWGG 3 cut(s) 246, 312, 753
BstNSI RCATGY 2 cut(s) 99, 253
BstSCI CCNGG 3 cut(s) 244, 310, 751
BstSFI CTRYAG 1 cut(s) 505
BstSLI GKGCMC 1 cut(s) 270
BstV2I GAAGAC 2 cut(s) 216, 602
BstX2I RGATCY 2 cut(s) 145, 808
BstYI RGATCY 2 cut(s) 145, 808
BtgI CCRYGG 1 cut(s) 271
BtsIMutI CAGTG 1 cut(s) 666
BveI ACCTGC 1 cut(s) 645
Cac8I GCNNGC 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 749
CseI GACGC 1 cut(s) 857
CviAII CATG 5 cut(s) 96, 250, 272, 723, 767
CviJI RGCY 6 cut(s) 82, 114, 338, 401, 692, 862
CviKI_1 RGCY 6 cut(s) 82, 114, 338, 401, 692, 862
DdeI CTNAG 2 cut(s) 180, 591
DpnI GATC 5 cut(s) 147, 206, 765, 775, 810
DpnII GATC 5 cut(s) 145, 204, 763, 773, 808
DraI TTTAAA 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 271
Eco31I GGTCTC 1 cut(s) 721
Eco47I GGWCC 1 cut(s) 749
EcoO109I RGGNCCY 1 cut(s) 749
EcoRI GAATTC 1 cut(s) 315
EcoRII CCWGG 3 cut(s) 244, 310, 751
EcoT14I CCWWGG 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 637
FaeI CATG 5 cut(s) 99, 253, 275, 726, 770
FaqI GGGAC 2 cut(s) 190, 683
FatI CATG 5 cut(s) 95, 249, 271, 722, 766
GsaI CCCAGC 2 cut(s) 53, 855
HgaI GACGC 1 cut(s) 857
Hin1I GRCGYC 1 cut(s) 646
Hin1II CATG 5 cut(s) 99, 253, 275, 726, 770
HinfI GANTC 1 cut(s) 178
Hpy166II GTNNAC 2 cut(s) 268, 758
Hpy188I TCNGA 4 cut(s) 70, 594, 778, 847
Hpy188III TCNNGA 2 cut(s) 416, 804
Hpy8I GTNNAC 2 cut(s) 268, 758
HpyAV CCTTC 1 cut(s) 779
HpyCH4III ACNGT 1 cut(s) 732
HpyCH4IV ACGT 1 cut(s) 646
HpyCH4V TGCA 8 cut(s) 167, 260, 268, 332, 488, 654, 735, 815
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 494, 565
HpyF3I CTNAG 2 cut(s) 180, 591
HpySE526I ACGT 1 cut(s) 646
Hsp92I GRCGYC 1 cut(s) 646
Hsp92II CATG 5 cut(s) 99, 253, 275, 726, 770
Kzo9I GATC 5 cut(s) 145, 204, 763, 773, 808
LmnI GCTCC 1 cut(s) 79
LweI GCATC 2 cut(s) 247, 372
MaeII ACGT 1 cut(s) 646
MaeIII GTNAC 3 cut(s) 370, 535, 629
MalI GATC 5 cut(s) 147, 206, 765, 775, 810
MboI GATC 5 cut(s) 145, 204, 763, 773, 808
MboII GAAGA 4 cut(s) 35, 221, 607, 783
MflI RGATCY 2 cut(s) 145, 808
MhlI GDGCHC 2 cut(s) 270, 415
MluCI AATT 9 cut(s) 162, 199, 315, 477, 513, 707, 738, 790, 830
MlyI GAGTC 1 cut(s) 172
MnlI CCTC 7 cut(s) 49, 383, 483, 570, 579, 600, 836
MseI TTAA 4 cut(s) 120, 171, 422, 710
MslI CAYNNNNRTG 1 cut(s) 368
MspR9I CCNGG 3 cut(s) 246, 312, 753
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 3 cut(s) 246, 312, 753
MwoI GCNNNNNNNGC 3 cut(s) 46, 494, 565
NcoI CCATGG 1 cut(s) 271
NdeII GATC 5 cut(s) 145, 204, 763, 773, 808
NlaIII CATG 5 cut(s) 99, 253, 275, 726, 770
NlaIV GGNNCC 1 cut(s) 750
NmuCI GTSAC 1 cut(s) 370
NspI RCATGY 2 cut(s) 99, 253
PaeI GCATGC 1 cut(s) 253
PctI GAATGC 1 cut(s) 379
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PpuMI RGGWCCY 1 cut(s) 749
PsiI TTATAA 2 cut(s) 161, 282
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 3 cut(s) 244, 310, 751
PspFI CCCAGC 2 cut(s) 49, 851
PspGI CCWGG 3 cut(s) 244, 310, 751
PspN4I GGNNCC 1 cut(s) 750
PspPI GGNCC 1 cut(s) 749
PspPPI RGGWCCY 1 cut(s) 749
PsuI RGATCY 2 cut(s) 145, 808
RseI CAYNNNNRTG 1 cut(s) 368
SaqAI TTAA 4 cut(s) 120, 171, 422, 710
Sau3AI GATC 5 cut(s) 145, 204, 763, 773, 808
Sau96I GGNCC 1 cut(s) 749
SchI GAGTC 1 cut(s) 172
ScrFI CCNGG 3 cut(s) 246, 312, 753
SduI GDGCHC 2 cut(s) 270, 415
SetI ASST 7 cut(s) 60, 84, 313, 475, 649, 659, 694
SfaNI GCATC 2 cut(s) 247, 372
SfcI CTRYAG 1 cut(s) 505
SinI GGWCC 1 cut(s) 749
SmiMI CAYNNNNRTG 1 cut(s) 368
SphI GCATGC 1 cut(s) 253
Sse9I AATT 9 cut(s) 162, 199, 315, 477, 513, 707, 738, 790, 830
SspI AATATT 1 cut(s) 492
StyD4I CCNGG 3 cut(s) 244, 310, 751
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 1 cut(s) 732
TaiI ACGT 1 cut(s) 649
TaqI TCGA 4 cut(s) 207, 475, 625, 705
TasI AATT 9 cut(s) 162, 199, 315, 477, 513, 707, 738, 790, 830
Tru1I TTAA 4 cut(s) 120, 171, 422, 710
Tru9I TTAA 4 cut(s) 120, 171, 422, 710
TscAI CASTG 1 cut(s) 666
TseFI GTSAC 1 cut(s) 370
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 1 cut(s) 393
TspGWI ACGGA 1 cut(s) 92
TspRI CASTG 1 cut(s) 666
VneI GTGCAC 1 cut(s) 266
VpaK11BI GGWCC 1 cut(s) 749
XapI RAATTY 3 cut(s) 315, 513, 790
XceI RCATGY 2 cut(s) 99, 253
ZraI GACGTC 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.