Rw2G024610

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002948
Physical Location & Seq
Forward (+)
36349616 .. 36353402
3787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024610.1

Sequence Viewer

Length: 1047 bp
ATGGAGAGAAGAAGGAGATGGTTGCTGTTGTTGGCACTACTTACTTGCCTCTTTGCTTCTTCTGCTGGGAGAGAATGGAAGTTCAAGCACAAGGTTCATAGCCAGAGCCCCATCTACAATCATACGCTTGCCACAATATTGGTTCGCTATGCCTCTACGGTGTATTTGTCAGATTTGACAGAGCTGTTTTCTTGGACATGCGACAGATGTGATGGCTTGATTAAGGACTTTCAAATGATAGAGATAATTGTGGACGTCCAGCATTGCTTACAGGCATTTGTTGGGGTGGCAACAGATCCAAATGCTATTATAATTGCATTTAAAGGGACTCAGGGAAACAGTATACAGAATTGGATCGAAGACTTATTTTGGAAACAACTTGATATAGATTACCCTGGCATGCCTGATGCAATGGTGCACCATGGTTTTTATAATGCTTACCACAACACAACCATACGACCTGGAATTCTAAATGCTGTTGCAAGAGCCAAAGAGTTTTATGGAGATATTGGCATCATAGTGACAGGGCATTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATTTAAGGGTTAATCAAAAGGAGAATAATGTTCAAGTTATGACATTTGGACAACCTCGAATCGGTAATGCAATTTTTGCTACTTACTATAGCGAAATTCTGCCACAGACTATACGAGTAACAAATGGAAATGATGTTGTGCCTCATATGCCTCCATACTACACTTATTTTCCTCAGAAGACATACCACCACTTCCCAACGGAGGTGTGGCTTTATAACATTGGATTGCGAAGTCTTGTTTATCAGGTTGAGAAAATATGTGATGCCTCTGGTGAGGACCCAACTTGCAGCAGGTCAGTGATAGGGGACAGCATTTCAGACCATTTAGTTTATTATGGTGTCAAATTAATGGATGAGACATGGAAACCGTGCAAAATTGTGACGGGTCCTGGTTTACTGGATCATGGAAGATCAGATCTGGAAGGAAACTTTGTTCTGTCCAGAGATCTTGCAACTCCTGTTCTCAAATTGAAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

348

Amino Acids

39.43

Weight (kDa)

6.49

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 104 - 242 2.1e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 311, 432, 783
AatII GACGTC 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 849
AccI GTMKAC 1 cut(s) 343
AclWI GGATC 3 cut(s) 290, 362, 975
AcsI RAATTY 2 cut(s) 465, 663
AcyI GRCGYC 1 cut(s) 255
AfiI CCNNNNNNNGG 2 cut(s) 629, 769
AgsI TTSAA 5 cut(s) 85, 233, 534, 602, 1039
AjnI CCWGG 3 cut(s) 394, 460, 955
AloI GAACNNNNNNTCC 2 cut(s) 984, 1016
AluBI AGCT 1 cut(s) 184
AluI AGCT 1 cut(s) 184
Alw21I GWGCWC 2 cut(s) 420, 565
Alw26I GTCTC 1 cut(s) 917
Alw44I GTGCAC 1 cut(s) 416
AlwI GGATC 3 cut(s) 290, 362, 975
ApaLI GTGCAC 1 cut(s) 416
ApeKI GCWGC 1 cut(s) 855
ApoI RAATTY 2 cut(s) 465, 663
AseI ATTAAT 1 cut(s) 914
AspS9I GGNCC 2 cut(s) 844, 953
AsuHPI GGTGA 1 cut(s) 851
AvaII GGWCC 2 cut(s) 844, 953
BaeGI GKGCMC 1 cut(s) 420
BanII GRGCYC 1 cut(s) 110
BbsI GAAGAC 2 cut(s) 366, 752
Bbv12I GWGCWC 2 cut(s) 420, 565
BbvI GCAGC 1 cut(s) 867
BccI CCATC 3 cut(s) 12, 119, 206
BciT130I CCWGG 3 cut(s) 396, 462, 957
BcoDI GTCTC 1 cut(s) 917
BfmI CTRYAG 1 cut(s) 655
BfuAI ACCTGC 1 cut(s) 849
BglII AGATCT 2 cut(s) 982, 1012
BisI GCNGC 1 cut(s) 856
BlsI GCNGC 1 cut(s) 857
Bme1390I CCNGG 3 cut(s) 396, 462, 957
Bme18I GGWCC 2 cut(s) 844, 953
BmgT120I GGNCC 2 cut(s) 844, 953
BmiI GGNNCC 2 cut(s) 846, 954
BmrFI CCNGG 3 cut(s) 396, 462, 957
BmsI GCATC 3 cut(s) 397, 522, 820
BpiI GAAGAC 2 cut(s) 366, 752
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 2 cut(s) 394, 421
Bsc4I CCNNNNNNNGG 2 cut(s) 629, 769
Bse1I ACTGG 1 cut(s) 969
Bse3DI GCAATG 3 cut(s) 262, 417, 552
BseBI CCWGG 3 cut(s) 396, 462, 957
BseDI CCNNGG 2 cut(s) 394, 421
BseGI GGATG 1 cut(s) 925
BseLI CCNNNNNNNGG 2 cut(s) 629, 769
BseMI GCAATG 3 cut(s) 262, 417, 552
BseMII CTCAG 2 cut(s) 344, 755
BseNI ACTGG 1 cut(s) 969
BseSI GKGCMC 1 cut(s) 420
BseXI GCAGC 1 cut(s) 867
BseYI CCCAGC 1 cut(s) 65
BsiHKAI GWGCWC 2 cut(s) 420, 565
BslFI GGGAC 2 cut(s) 340, 887
BslI CCNNNNNNNGG 2 cut(s) 629, 769
BsmAI GTCTC 1 cut(s) 917
BsmFI GGGAC 2 cut(s) 340, 887
BsmI GAATGC 1 cut(s) 529
Bsp1286I GDGCHC 3 cut(s) 110, 420, 565
Bsp143I GATC 6 cut(s) 295, 354, 967, 977, 982, 1012
Bsp19I CCATGG 1 cut(s) 421
BspCNI CTCAG 2 cut(s) 343, 754
BspLI GGNNCC 2 cut(s) 846, 954
BspMI ACCTGC 1 cut(s) 849
BspPI GGATC 3 cut(s) 290, 362, 975
BsrDI GCAATG 3 cut(s) 262, 417, 552
BsrI ACTGG 1 cut(s) 969
BssECI CCNNGG 2 cut(s) 394, 421
BssMI GATC 6 cut(s) 295, 354, 967, 977, 982, 1012
BssNAI GTATAC 1 cut(s) 344
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 1 cut(s) 421
Bst1107I GTATAC 1 cut(s) 344
Bst2UI CCWGG 3 cut(s) 396, 462, 957
Bst4CI ACNGT 3 cut(s) 160, 341, 936
BstACI GRCGYC 1 cut(s) 255
BstAPI GCANNNNNTGC 1 cut(s) 644
BstC8I GCNNGC 2 cut(s) 129, 401
BstDEI CTNAG 2 cut(s) 330, 741
BstDSI CCRYGG 1 cut(s) 421
BstF5I GGATG 1 cut(s) 925
BstKTI GATC 6 cut(s) 298, 357, 970, 980, 985, 1015
BstMAI GTCTC 1 cut(s) 917
BstMBI GATC 6 cut(s) 295, 354, 967, 977, 982, 1012
BstMWI GCNNNNNNNGC 3 cut(s) 62, 644, 715
BstNI CCWGG 3 cut(s) 396, 462, 957
BstNSI RCATGY 2 cut(s) 201, 403
BstSCI CCNGG 3 cut(s) 394, 460, 955
BstSFI CTRYAG 1 cut(s) 655
BstSLI GKGCMC 1 cut(s) 420
BstV1I GCAGC 1 cut(s) 867
BstV2I GAAGAC 2 cut(s) 366, 752
BstX2I RGATCY 3 cut(s) 295, 982, 1012
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 3 cut(s) 295, 982, 1012
BstZ17I GTATAC 1 cut(s) 344
BtgI CCRYGG 1 cut(s) 421
BtsCI GGATG 1 cut(s) 925
BtsIMutI CAGTG 1 cut(s) 870
BveI ACCTGC 1 cut(s) 849
Cac8I GCNNGC 2 cut(s) 129, 401
Cfr13I GGNCC 2 cut(s) 844, 953
CviAII CATG 5 cut(s) 198, 400, 422, 927, 971
CviJI RGCY 7 cut(s) 102, 108, 184, 216, 488, 551, 778
CviKI_1 RGCY 7 cut(s) 102, 108, 184, 216, 488, 551, 778
DdeI CTNAG 2 cut(s) 330, 741
DpnI GATC 6 cut(s) 297, 356, 969, 979, 984, 1014
DpnII GATC 6 cut(s) 295, 354, 967, 977, 982, 1012
DraI TTTAAA 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 421
Eco24I GRGCYC 1 cut(s) 110
Eco47I GGWCC 2 cut(s) 844, 953
EcoO109I RGGNCCY 2 cut(s) 844, 953
EcoRI GAATTC 1 cut(s) 465
EcoRII CCWGG 3 cut(s) 394, 460, 955
EcoT14I CCWWGG 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 110
ErhI CCWWGG 1 cut(s) 421
FaeI CATG 5 cut(s) 201, 403, 425, 930, 974
FaqI GGGAC 2 cut(s) 340, 887
FatI CATG 5 cut(s) 197, 399, 421, 926, 970
FauNDI CATATG 1 cut(s) 714
FblI GTMKAC 1 cut(s) 343
Fnu4HI GCNGC 1 cut(s) 856
FokI GGATG 1 cut(s) 932
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 1 cut(s) 856
GluI GCNGC 1 cut(s) 856
GsaI CCCAGC 1 cut(s) 69
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 5 cut(s) 201, 403, 425, 930, 974
HinfI GANTC 2 cut(s) 328, 627
HphI GGTGA 1 cut(s) 851
Hpy166II GTNNAC 4 cut(s) 253, 344, 418, 962
Hpy188I TCNGA 4 cut(s) 172, 744, 886, 982
Hpy188III TCNNGA 3 cut(s) 566, 986, 1008
Hpy8I GTNNAC 4 cut(s) 253, 344, 418, 962
HpyAV CCTTC 2 cut(s) 6, 983
HpyCH4III ACNGT 3 cut(s) 160, 341, 936
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 8 cut(s) 317, 410, 418, 482, 638, 855, 939, 1019
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 644, 715
HpyF3I CTNAG 2 cut(s) 330, 741
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 5 cut(s) 201, 403, 425, 930, 974
Kzo9I GATC 6 cut(s) 295, 354, 967, 977, 982, 1012
Lsp1109I GCAGC 1 cut(s) 867
LweI GCATC 3 cut(s) 397, 522, 820
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 520, 685, 946
MalI GATC 6 cut(s) 297, 356, 969, 979, 984, 1014
MboI GATC 6 cut(s) 295, 354, 967, 977, 982, 1012
MboII GAAGA 5 cut(s) 21, 51, 371, 757, 987
MflI RGATCY 3 cut(s) 295, 982, 1012
MhlI GDGCHC 3 cut(s) 110, 420, 565
MluCI AATT 9 cut(s) 246, 312, 349, 465, 639, 663, 911, 942, 1034
MlyI GAGTC 1 cut(s) 322
MseI TTAA 5 cut(s) 222, 321, 572, 579, 914
MslI CAYNNNNRTG 1 cut(s) 518
MspR9I CCNGG 3 cut(s) 396, 462, 957
Mva1269I GAATGC 1 cut(s) 529
MvaI CCWGG 3 cut(s) 396, 462, 957
MwoI GCNNNNNNNGC 3 cut(s) 62, 644, 715
NcoI CCATGG 1 cut(s) 421
NdeI CATATG 1 cut(s) 714
NdeII GATC 6 cut(s) 295, 354, 967, 977, 982, 1012
NlaIII CATG 5 cut(s) 201, 403, 425, 930, 974
NlaIV GGNNCC 2 cut(s) 846, 954
NmuCI GTSAC 2 cut(s) 520, 946
NspI RCATGY 2 cut(s) 201, 403
PaeI GCATGC 1 cut(s) 403
PctI GAATGC 1 cut(s) 529
PfeI GAWTC 1 cut(s) 627
PkrI GCNGC 1 cut(s) 857
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PpuMI RGGWCCY 2 cut(s) 844, 953
PshBI ATTAAT 1 cut(s) 914
PsiI TTATAA 3 cut(s) 311, 432, 783
Psp5II RGGWCCY 2 cut(s) 844, 953
Psp6I CCWGG 3 cut(s) 394, 460, 955
PspFI CCCAGC 1 cut(s) 65
PspGI CCWGG 3 cut(s) 394, 460, 955
PspN4I GGNNCC 2 cut(s) 846, 954
PspPI GGNCC 2 cut(s) 844, 953
PspPPI RGGWCCY 2 cut(s) 844, 953
PsuI RGATCY 3 cut(s) 295, 982, 1012
RseI CAYNNNNRTG 1 cut(s) 518
SaqAI TTAA 5 cut(s) 222, 321, 572, 579, 914
SatI GCNGC 1 cut(s) 856
Sau3AI GATC 6 cut(s) 295, 354, 967, 977, 982, 1012
Sau96I GGNCC 2 cut(s) 844, 953
SchI GAGTC 1 cut(s) 322
ScrFI CCNGG 3 cut(s) 396, 462, 957
SduI GDGCHC 3 cut(s) 110, 420, 565
SetI ASST 8 cut(s) 96, 186, 258, 463, 625, 774, 816, 863
SfaNI GCATC 3 cut(s) 397, 522, 820
SfcI CTRYAG 1 cut(s) 655
SinI GGWCC 2 cut(s) 844, 953
SmiMI CAYNNNNRTG 1 cut(s) 518
SphI GCATGC 1 cut(s) 403
Sse9I AATT 9 cut(s) 246, 312, 349, 465, 639, 663, 911, 942, 1034
SspI AATATT 1 cut(s) 138
StyD4I CCNGG 3 cut(s) 394, 460, 955
StyI CCWWGG 1 cut(s) 421
TaaI ACNGT 3 cut(s) 160, 341, 936
TaiI ACGT 1 cut(s) 258
TaqI TCGA 2 cut(s) 357, 625
TasI AATT 9 cut(s) 246, 312, 349, 465, 639, 663, 911, 942, 1034
TfiI GAWTC 1 cut(s) 627
Tru1I TTAA 5 cut(s) 222, 321, 572, 579, 914
Tru9I TTAA 5 cut(s) 222, 321, 572, 579, 914
TscAI CASTG 1 cut(s) 870
TseFI GTSAC 2 cut(s) 520, 946
TseI GCWGC 1 cut(s) 855
Tsp45I GTSAC 2 cut(s) 520, 946
TspDTI ATGAA 2 cut(s) 86, 543
TspGWI ACGGA 1 cut(s) 782
TspRI CASTG 1 cut(s) 870
VneI GTGCAC 1 cut(s) 416
VpaK11BI GGWCC 2 cut(s) 844, 953
VspI ATTAAT 1 cut(s) 914
XapI RAATTY 2 cut(s) 465, 663
XceI RCATGY 2 cut(s) 201, 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 771
XmiI GTMKAC 1 cut(s) 343
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.