Rh2BG315200

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002948
Physical Location & Seq
Forward (+)
37108780 .. 37113717
4938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG315200.1

Sequence Viewer

Length: 870 bp
ATGATCATGGAGAGAAGAAGGAGATGGTTGCTGTTGTTGGCACTACTTACTTGCCTCTTTGCTTCTTCTGCTGGGAGAGAATGGAAGTTCAAGCACAAGGTTCATAGCCAGAGCCCTGTCTACAATCATACGCTTGCCACAATATTGGTTCGCTATGCCTCTACAGTGTATTTGTCAGATTTGACAGAGCTGTTTTCTTGGACATGCGACAGATGTGATGGCTTGATTAAGGATTTTCAAATGATAGAGATAATTGTGGACGTCCAGCATTGCTTACAGGCATTTGTTGGGGTGGCAACAGATCCAAATGCTATTATAATTGCATTTAAAGGGACTCAGGGAAACAGTATACAGAATTGGATCGAAGACTTATTTTGGAAACAACTTGATATAGATTACCCTGGCATGCCTGATGCAATGGTGCACCATGGTTTTTATAATGCTTACCACAACACAACCATACGACCTGGAATTCTAAATGCTGTTGCAAGAGCCAAAGAGTTTTATGGAGATATTGGCATCATAGTGACAGGGCATTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATTTAAGGGTTAATCAAAAGGAGAATAATGTTCAAGTTATGACATTTGGACAACCTCGAATCGGTAATGCAATTTTTGCTACTTACTATAGCGAAATTCTGCCACAGACTATACGAGTAACAAATGGAAATGATGTTGTGCCTCATATGCCTCCATACTACACTTATTTTCCTCAGAAGACATACCACCACTTCCCAACAGAGGTGTGGCTTTATAACATTGGATTGCGAAGTCTTGTTTATCAGGTTGAGAAAATATGTGATGCCTCTGGTGAGGACCCAACCTGCAGCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

289

Amino Acids

32.97

Weight (kDa)

6.58

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 106 - 244 1.4e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 317, 438, 789
AatII GACGTC 1 cut(s) 264
Acc36I ACCTGC 2 cut(s) 855, 866
AccI GTMKAC 2 cut(s) 120, 349
AclWI GGATC 2 cut(s) 296, 368
AcsI RAATTY 2 cut(s) 471, 669
AcyI GRCGYC 1 cut(s) 261
AfiI CCNNNNNNNGG 3 cut(s) 635, 775, 864
AgsI TTSAA 4 cut(s) 91, 239, 540, 608
AjnI CCWGG 2 cut(s) 400, 466
AluBI AGCT 1 cut(s) 190
AluI AGCT 1 cut(s) 190
Alw21I GWGCWC 2 cut(s) 426, 571
Alw44I GTGCAC 1 cut(s) 422
AlwI GGATC 2 cut(s) 296, 368
ApaLI GTGCAC 1 cut(s) 422
ApeKI GCWGC 1 cut(s) 861
ApoI RAATTY 2 cut(s) 471, 669
AspS9I GGNCC 1 cut(s) 850
AsuHPI GGTGA 1 cut(s) 857
AvaII GGWCC 1 cut(s) 850
BaeGI GKGCMC 1 cut(s) 426
BanII GRGCYC 1 cut(s) 116
BbsI GAAGAC 2 cut(s) 372, 758
Bbv12I GWGCWC 2 cut(s) 426, 571
BccI CCATC 2 cut(s) 18, 212
BciT130I CCWGG 2 cut(s) 402, 468
BclI TGATCA 1 cut(s) 3
BfmI CTRYAG 3 cut(s) 162, 661, 859
BfuAI ACCTGC 2 cut(s) 855, 866
BisI GCNGC 1 cut(s) 862
BlsI GCNGC 1 cut(s) 863
Bme1390I CCNGG 2 cut(s) 402, 468
Bme18I GGWCC 1 cut(s) 850
BmgT120I GGNCC 1 cut(s) 850
BmiI GGNNCC 1 cut(s) 852
BmrFI CCNGG 2 cut(s) 402, 468
BmsI GCATC 3 cut(s) 403, 528, 826
BpiI GAAGAC 2 cut(s) 372, 758
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 2 cut(s) 400, 427
Bsc4I CCNNNNNNNGG 3 cut(s) 635, 775, 864
Bse3DI GCAATG 3 cut(s) 268, 423, 558
BseBI CCWGG 2 cut(s) 402, 468
BseDI CCNNGG 2 cut(s) 400, 427
BseLI CCNNNNNNNGG 3 cut(s) 635, 775, 864
BseMI GCAATG 3 cut(s) 268, 423, 558
BseMII CTCAG 2 cut(s) 350, 761
BseSI GKGCMC 1 cut(s) 426
BseYI CCCAGC 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 426, 571
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 3 cut(s) 635, 775, 864
BsmFI GGGAC 1 cut(s) 346
BsmI GAATGC 1 cut(s) 535
Bsp1286I GDGCHC 3 cut(s) 116, 426, 571
Bsp143I GATC 3 cut(s) 3, 301, 360
Bsp19I CCATGG 1 cut(s) 427
BspCNI CTCAG 2 cut(s) 349, 760
BspLI GGNNCC 1 cut(s) 852
BspMAI CTGCAG 1 cut(s) 863
BspMI ACCTGC 2 cut(s) 855, 866
BspPI GGATC 2 cut(s) 296, 368
BsrDI GCAATG 3 cut(s) 268, 423, 558
BssECI CCNNGG 2 cut(s) 400, 427
BssMI GATC 3 cut(s) 3, 301, 360
BssNAI GTATAC 1 cut(s) 350
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 427
Bst1107I GTATAC 1 cut(s) 350
Bst2UI CCWGG 2 cut(s) 402, 468
Bst4CI ACNGT 2 cut(s) 166, 347
BstACI GRCGYC 1 cut(s) 261
BstAPI GCANNNNNTGC 1 cut(s) 650
BstC8I GCNNGC 2 cut(s) 135, 407
BstDEI CTNAG 2 cut(s) 336, 747
BstDSI CCRYGG 1 cut(s) 427
BstENI CCTNNNNNAGG 1 cut(s) 862
BstKTI GATC 3 cut(s) 6, 304, 363
BstMBI GATC 3 cut(s) 3, 301, 360
BstMWI GCNNNNNNNGC 3 cut(s) 68, 650, 721
BstNI CCWGG 2 cut(s) 402, 468
BstNSI RCATGY 2 cut(s) 207, 409
BstSCI CCNGG 2 cut(s) 400, 466
BstSFI CTRYAG 3 cut(s) 162, 661, 859
BstSLI GKGCMC 1 cut(s) 426
BstV2I GAAGAC 2 cut(s) 372, 758
BstX2I RGATCY 1 cut(s) 301
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 1 cut(s) 301
BstZ17I GTATAC 1 cut(s) 350
BtgI CCRYGG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 171
BveI ACCTGC 2 cut(s) 855, 866
Cac8I GCNNGC 2 cut(s) 135, 407
Cfr13I GGNCC 1 cut(s) 850
CviAII CATG 4 cut(s) 7, 204, 406, 428
CviJI RGCY 7 cut(s) 108, 114, 190, 222, 494, 557, 784
CviKI_1 RGCY 7 cut(s) 108, 114, 190, 222, 494, 557, 784
DdeI CTNAG 2 cut(s) 336, 747
DpnI GATC 3 cut(s) 5, 303, 362
DpnII GATC 3 cut(s) 3, 301, 360
DraI TTTAAA 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 427
Eco24I GRGCYC 1 cut(s) 116
Eco47I GGWCC 1 cut(s) 850
EcoNI CCTNNNNNAGG 1 cut(s) 862
EcoO109I RGGNCCY 1 cut(s) 850
EcoRI GAATTC 1 cut(s) 471
EcoRII CCWGG 2 cut(s) 400, 466
EcoT14I CCWWGG 1 cut(s) 427
EcoT38I GRGCYC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 4 cut(s) 10, 207, 409, 431
FaqI GGGAC 1 cut(s) 346
FatI CATG 4 cut(s) 6, 203, 405, 427
FauNDI CATATG 1 cut(s) 720
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 120, 349
Fnu4HI GCNGC 1 cut(s) 862
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 1 cut(s) 862
GluI GCNGC 1 cut(s) 862
GsaI CCCAGC 1 cut(s) 75
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 4 cut(s) 10, 207, 409, 431
HinfI GANTC 2 cut(s) 334, 633
HphI GGTGA 1 cut(s) 857
Hpy166II GTNNAC 4 cut(s) 121, 259, 350, 424
Hpy188I TCNGA 2 cut(s) 178, 750
Hpy188III TCNNGA 1 cut(s) 572
Hpy8I GTNNAC 4 cut(s) 121, 259, 350, 424
HpyAV CCTTC 1 cut(s) 12
HpyCH4III ACNGT 2 cut(s) 166, 347
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 6 cut(s) 323, 416, 424, 488, 644, 861
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 650, 721
HpyF3I CTNAG 2 cut(s) 336, 747
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 4 cut(s) 10, 207, 409, 431
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 301, 360
LweI GCATC 3 cut(s) 403, 528, 826
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 526, 691
MalI GATC 3 cut(s) 5, 303, 362
MboI GATC 3 cut(s) 3, 301, 360
MboII GAAGA 4 cut(s) 27, 57, 377, 763
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 3 cut(s) 116, 426, 571
MluCI AATT 6 cut(s) 252, 318, 355, 471, 645, 669
MlyI GAGTC 1 cut(s) 328
MseI TTAA 4 cut(s) 228, 327, 578, 585
MslI CAYNNNNRTG 1 cut(s) 524
MspR9I CCNGG 2 cut(s) 402, 468
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 2 cut(s) 402, 468
MwoI GCNNNNNNNGC 3 cut(s) 68, 650, 721
NcoI CCATGG 1 cut(s) 427
NdeI CATATG 1 cut(s) 720
NdeII GATC 3 cut(s) 3, 301, 360
NlaIII CATG 4 cut(s) 10, 207, 409, 431
NlaIV GGNNCC 1 cut(s) 852
NmuCI GTSAC 1 cut(s) 526
NspI RCATGY 2 cut(s) 207, 409
PaeI GCATGC 1 cut(s) 409
PctI GAATGC 1 cut(s) 535
PfeI GAWTC 1 cut(s) 633
PkrI GCNGC 1 cut(s) 863
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
PpuMI RGGWCCY 1 cut(s) 850
PsiI TTATAA 3 cut(s) 317, 438, 789
Psp5II RGGWCCY 1 cut(s) 850
Psp6I CCWGG 2 cut(s) 400, 466
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 2 cut(s) 400, 466
PspN4I GGNNCC 1 cut(s) 852
PspPI GGNCC 1 cut(s) 850
PspPPI RGGWCCY 1 cut(s) 850
PstI CTGCAG 1 cut(s) 863
PsuI RGATCY 1 cut(s) 301
RseI CAYNNNNRTG 1 cut(s) 524
SaqAI TTAA 4 cut(s) 228, 327, 578, 585
SatI GCNGC 1 cut(s) 862
Sau3AI GATC 3 cut(s) 3, 301, 360
Sau96I GGNCC 1 cut(s) 850
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 2 cut(s) 402, 468
SduI GDGCHC 3 cut(s) 116, 426, 571
SetI ASST 9 cut(s) 102, 192, 264, 469, 631, 780, 822, 860, 869
SfaNI GCATC 3 cut(s) 403, 528, 826
SfcI CTRYAG 3 cut(s) 162, 661, 859
SinI GGWCC 1 cut(s) 850
SmiMI CAYNNNNRTG 1 cut(s) 524
SphI GCATGC 1 cut(s) 409
Sse9I AATT 6 cut(s) 252, 318, 355, 471, 645, 669
SspI AATATT 1 cut(s) 144
StyD4I CCNGG 2 cut(s) 400, 466
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 2 cut(s) 166, 347
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 363, 631
TasI AATT 6 cut(s) 252, 318, 355, 471, 645, 669
TfiI GAWTC 1 cut(s) 633
Tru1I TTAA 4 cut(s) 228, 327, 578, 585
Tru9I TTAA 4 cut(s) 228, 327, 578, 585
TscAI CASTG 1 cut(s) 171
TseFI GTSAC 1 cut(s) 526
TseI GCWGC 1 cut(s) 861
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 2 cut(s) 92, 549
TspRI CASTG 1 cut(s) 171
VneI GTGCAC 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 850
XagI CCTNNNNNAGG 1 cut(s) 862
XapI RAATTY 2 cut(s) 471, 669
XceI RCATGY 2 cut(s) 207, 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 777
XmiI GTMKAC 2 cut(s) 120, 349
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.