MD16G1174000.v1.1

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002948
Physical Location & Seq
Forward (+)
14718695 .. 14721824
3130 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1174000.v1.1.491

Sequence Viewer

Length: 729 bp
ATGTTTGTTTTTTCTGCTGGAAGAGAACTGAAGATTAAGCACAAGGATCATGGCCCCATATACAATCACACGCTTGCCACTATACTAGTGCGGTATGCTTCTACGGTTTATGTGTCTGATTTGTCAGAGCTGTTTACTTGGACATGCTCATGCTGTAATGGTTTGATTAAGGGATTTGAAATGATAGAGATTATTGTTGACGTCCAGCATTGCTTACAGGCTTTTGTTGGAGTGGCACAAGACCCGAATGCTATTATTATTGCATTTAGAGGGACTCAGGAACACAGCATACAGAATTGGATTGAAGACTTATTTTGGAAACAACTTGATCTAGATTATCCTGGCATGCCTGATTCAATGGTGCACCATGGGTTTTATAATGCTTACCATAACACGACCATACGCCCTGGAATTCTAAATGCTGTGGAAAGAGCCAGGGAATATTATGGAGATATTGGCATCATAGTTACAGGACATTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATCTAAGGGTCAATCAAAAAGAGAAAGATGTTCAGGTTATGACATTTGGACAACCTCGTATCGGTAATGCAGCTTTTGCATCATACTATAGCACACTTGTACCAAACACTTTACGAGTAACAAATTCACATGACATGGTGCCTCATTTGCCTCCATACTATACTTATTTTCCTCAGAAGACATACCACCACTTTCCACGAGAGGTATTCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

243

Amino Acids

27.56

Weight (kDa)

6.33

Isoelectric Point (pI)

28.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 86 - 224 4.5e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 378
AatII GACGTC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 652
AciI CCGC 1 cut(s) 91
AclWI GGATC 1 cut(s) 54
AcsI RAATTY 2 cut(s) 411, 637
AcuI CTGAAG 1 cut(s) 50
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 1 cut(s) 615
AfiI CCNNNNNNNGG 1 cut(s) 575
AgsI TTSAA 4 cut(s) 179, 305, 357, 480
AhlI ACTAGT 1 cut(s) 85
AjnI CCWGG 3 cut(s) 340, 406, 434
AluBI AGCT 2 cut(s) 130, 587
AluI AGCT 2 cut(s) 130, 587
Alw21I GWGCWC 2 cut(s) 366, 511
Alw44I GTGCAC 1 cut(s) 362
AlwI GGATC 1 cut(s) 54
AoxI GGCC 1 cut(s) 52
ApaLI GTGCAC 1 cut(s) 362
ApeKI GCWGC 1 cut(s) 584
ApoI RAATTY 2 cut(s) 411, 637
AspS9I GGNCC 1 cut(s) 53
BaeGI GKGCMC 1 cut(s) 366
BaeI ACNNNNGTAYC 2 cut(s) 597, 630
BanI GGYRCC 1 cut(s) 652
BauI CACGAG 1 cut(s) 711
BbsI GAAGAC 2 cut(s) 312, 698
Bbv12I GWGCWC 2 cut(s) 366, 511
BbvI GCAGC 1 cut(s) 596
BciT130I CCWGG 3 cut(s) 342, 408, 436
BcuI ACTAGT 1 cut(s) 85
BfaI CTAG 2 cut(s) 86, 332
BfmI CTRYAG 1 cut(s) 601
BisI GCNGC 1 cut(s) 585
BlsI GCNGC 1 cut(s) 586
Bme1390I CCNGG 3 cut(s) 342, 408, 436
BmgT120I GGNCC 1 cut(s) 53
BmiI GGNNCC 2 cut(s) 55, 654
BmrFI CCNGG 3 cut(s) 342, 408, 436
BmsI GCATC 2 cut(s) 468, 602
BpiI GAAGAC 2 cut(s) 312, 698
BplI GAGNNNNNCTC 1 cut(s) 707
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 3 cut(s) 367, 406, 435
Bsc4I CCNNNNNNNGG 1 cut(s) 575
Bse3DI GCAATG 2 cut(s) 208, 498
BseBI CCWGG 3 cut(s) 342, 408, 436
BseDI CCNNGG 3 cut(s) 367, 406, 435
BseLI CCNNNNNNNGG 1 cut(s) 575
BseMI GCAATG 2 cut(s) 208, 498
BseMII CTCAG 2 cut(s) 290, 701
BseSI GKGCMC 1 cut(s) 366
BseXI GCAGC 1 cut(s) 596
BshFI GGCC 1 cut(s) 54
BshNI GGYRCC 1 cut(s) 652
BsiHKAI GWGCWC 2 cut(s) 366, 511
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 1 cut(s) 575
BsmFI GGGAC 1 cut(s) 286
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 1 cut(s) 54
Bsp1286I GDGCHC 2 cut(s) 366, 511
Bsp143I GATC 3 cut(s) 46, 328, 514
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 1 cut(s) 91
BspANI GGCC 1 cut(s) 54
BspCNI CTCAG 2 cut(s) 289, 700
BspLI GGNNCC 2 cut(s) 55, 654
BspPI GGATC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 652
BsrDI GCAATG 2 cut(s) 208, 498
BssECI CCNNGG 3 cut(s) 367, 406, 435
BssMI GATC 3 cut(s) 46, 328, 514
BssNI GRCGYC 1 cut(s) 201
BssSI CACGAG 1 cut(s) 711
BssT1I CCWWGG 1 cut(s) 367
Bst2BI CACGAG 1 cut(s) 711
Bst2UI CCWGG 3 cut(s) 342, 408, 436
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 1 cut(s) 16
BstACI GRCGYC 1 cut(s) 201
BstAPI GCANNNNNTGC 1 cut(s) 590
BstC8I GCNNGC 2 cut(s) 75, 347
BstDEI CTNAG 4 cut(s) 276, 518, 687, 726
BstDSI CCRYGG 1 cut(s) 367
BstKTI GATC 3 cut(s) 49, 331, 517
BstMBI GATC 3 cut(s) 46, 328, 514
BstMWI GCNNNNNNNGC 2 cut(s) 590, 661
BstNI CCWGG 3 cut(s) 342, 408, 436
BstNSI RCATGY 2 cut(s) 147, 349
BstSCI CCNGG 3 cut(s) 340, 406, 434
BstSFI CTRYAG 1 cut(s) 601
BstSLI GKGCMC 1 cut(s) 366
BstV1I GCAGC 1 cut(s) 596
BstV2I GAAGAC 2 cut(s) 312, 698
BsuRI GGCC 1 cut(s) 54
BtgI CCRYGG 1 cut(s) 367
Cac8I GCNNGC 2 cut(s) 75, 347
Cfr13I GGNCC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 614
CviAII CATG 7 cut(s) 50, 144, 150, 346, 368, 644, 649
CviJI RGCY 6 cut(s) 54, 130, 221, 434, 497, 587
CviKI_1 RGCY 6 cut(s) 54, 130, 221, 434, 497, 587
CviQI GTAC 1 cut(s) 614
DdeI CTNAG 4 cut(s) 276, 518, 687, 726
DpnI GATC 3 cut(s) 48, 330, 516
DpnII GATC 3 cut(s) 46, 328, 514
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco130I CCWWGG 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 50
EcoRI GAATTC 1 cut(s) 411
EcoRII CCWGG 3 cut(s) 340, 406, 434
EcoT14I CCWWGG 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 7 cut(s) 53, 147, 153, 349, 371, 647, 652
FaqI GGGAC 1 cut(s) 286
FatI CATG 7 cut(s) 49, 143, 149, 345, 367, 643, 648
Fnu4HI GCNGC 1 cut(s) 585
Fsp4HI GCNGC 1 cut(s) 585
FspBI CTAG 2 cut(s) 86, 332
GluI GCNGC 1 cut(s) 585
HaeIII GGCC 1 cut(s) 54
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 7 cut(s) 53, 147, 153, 349, 371, 647, 652
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 2 cut(s) 274, 353
Hpy166II GTNNAC 3 cut(s) 135, 199, 364
Hpy188I TCNGA 3 cut(s) 118, 127, 690
Hpy188III TCNNGA 3 cut(s) 278, 332, 512
Hpy8I GTNNAC 3 cut(s) 135, 199, 364
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 4 cut(s) 263, 364, 584, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 590, 661
HpyF3I CTNAG 4 cut(s) 276, 518, 687, 726
HpySE526I ACGT 1 cut(s) 201
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 7 cut(s) 53, 147, 153, 349, 371, 647, 652
Kzo9I GATC 3 cut(s) 46, 328, 514
Lsp1109I GCAGC 1 cut(s) 596
LweI GCATC 2 cut(s) 468, 602
MaeI CTAG 2 cut(s) 86, 332
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 2 cut(s) 466, 631
MalI GATC 3 cut(s) 48, 330, 516
MboI GATC 3 cut(s) 46, 328, 514
MboII GAAGA 4 cut(s) 33, 43, 317, 703
MhlI GDGCHC 2 cut(s) 366, 511
MluCI AATT 3 cut(s) 295, 411, 637
MlyI GAGTC 1 cut(s) 268
MmeI TCCRAC 1 cut(s) 208
MnlI CCTC 7 cut(s) 263, 479, 579, 666, 675, 696, 709
MseI TTAA 2 cut(s) 36, 168
MslI CAYNNNNRTG 1 cut(s) 148
MspR9I CCNGG 3 cut(s) 342, 408, 436
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 3 cut(s) 342, 408, 436
MwoI GCNNNNNNNGC 2 cut(s) 590, 661
NcoI CCATGG 1 cut(s) 367
NdeII GATC 3 cut(s) 46, 328, 514
NlaIII CATG 7 cut(s) 53, 147, 153, 349, 371, 647, 652
NlaIV GGNNCC 2 cut(s) 55, 654
NspI RCATGY 2 cut(s) 147, 349
PaeI GCATGC 1 cut(s) 349
PctI GAATGC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 353
PkrI GCNGC 1 cut(s) 586
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PsiI TTATAA 1 cut(s) 378
Psp6I CCWGG 3 cut(s) 340, 406, 434
PspGI CCWGG 3 cut(s) 340, 406, 434
PspN4I GGNNCC 2 cut(s) 55, 654
PspPI GGNCC 1 cut(s) 53
PsrI GAACNNNNNNTAC 2 cut(s) 273, 305
RsaI GTAC 1 cut(s) 615
RsaNI GTAC 1 cut(s) 614
RseI CAYNNNNRTG 1 cut(s) 148
SaqAI TTAA 2 cut(s) 36, 168
SatI GCNGC 1 cut(s) 585
Sau3AI GATC 3 cut(s) 46, 328, 514
Sau96I GGNCC 1 cut(s) 53
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 3 cut(s) 342, 408, 436
SduI GDGCHC 2 cut(s) 366, 511
SetI ASST 6 cut(s) 132, 204, 552, 571, 589, 720
SfaNI GCATC 2 cut(s) 468, 602
SfcI CTRYAG 1 cut(s) 601
SmiMI CAYNNNNRTG 1 cut(s) 148
SpeI ACTAGT 1 cut(s) 85
SphI GCATGC 1 cut(s) 349
Sse9I AATT 3 cut(s) 295, 411, 637
SsiI CCGC 1 cut(s) 91
SspI AATATT 1 cut(s) 443
SspMI CTAG 2 cut(s) 86, 332
StyD4I CCNGG 3 cut(s) 340, 406, 434
StyI CCWWGG 1 cut(s) 367
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 1 cut(s) 204
TasI AATT 3 cut(s) 295, 411, 637
TfiI GAWTC 1 cut(s) 353
Tru1I TTAA 2 cut(s) 36, 168
Tru9I TTAA 2 cut(s) 36, 168
TseI GCWGC 1 cut(s) 584
TspDTI ATGAA 1 cut(s) 489
VneI GTGCAC 1 cut(s) 362
XapI RAATTY 2 cut(s) 411, 637
XbaI TCTAGA 1 cut(s) 331
XceI RCATGY 2 cut(s) 147, 349
XspI CTAG 2 cut(s) 86, 332
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.