Rorug04G0169100

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002948
Physical Location & Seq
Forward (+)
29824403 .. 29828351
3949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0169100.1

Sequence Viewer

Length: 636 bp
ATGGAGGATGCTATTTTGAATGAAGATGAAAAGACTGTATCCGAAATTGAGGCAAGGATAGAAGTTGCAAAGGAAGCCTCTAATATGGTATTAGCAGATTACAATTTTAGCACTATAGTTTCTGTTGTTGGAGAAGGCATATCTATTTACAACAACATGAAAGCTTTTATCAGGTATATATGCAGTTTTTCCATGAGTTTCACCAGCGTCCATGGTAAGAGTCCATCCACGGAATATGCAAAGTTGAGGAAGGATTCACTAGAAAGTGAATTTGGACATGCTCTTGGAGCTAATCCCTCCAGAATTTTTTCTGCTGCCTACTGTTTTGGTCCATTTTTGGCTTTGTGTAGACCAGCAATTATTTTGTTTCATGTGTTGAAGCTAACAATCTGGCAATTCTTTGTTCAAGACATAAGGAAACGTGCCATCAAGCTAACAATCTGGCAATTCTTTGTTCAAGACATAAGGAAACGTGCCATCAAGCAATGTGAATATTCAAATGTTAAAGCTACTGAACTGATTCTCCCACAGTTTGTTAACCAATATTACATCATAGTGGTGGCAGAAGCAGAAGAAAAAAAAGAGGAGCAGCAGGAGCAGGCATTAGTAGTAGCCGAAGTGGGCAAGCAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

211

Amino Acids

24.06

Weight (kDa)

6.0

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 349
AcsI RAATTY 2 cut(s) 269, 303
AgsI TTSAA 5 cut(s) 19, 379, 407, 458, 498
AjuI GAANNNNNNNTTGG 2 cut(s) 255, 287
AloI GAACNNNNNNTCC 2 cut(s) 507, 539
AluBI AGCT 5 cut(s) 164, 290, 382, 433, 509
AluI AGCT 5 cut(s) 164, 290, 382, 433, 509
ApeKI GCWGC 2 cut(s) 314, 589
ApoI RAATTY 2 cut(s) 269, 303
Asp700I GAANNNNTTC 2 cut(s) 307, 519
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 1 cut(s) 193
AvaII GGWCC 1 cut(s) 329
BbvI GCAGC 2 cut(s) 301, 601
BccI CCATC 3 cut(s) 232, 434, 485
BciVI GTATCC 1 cut(s) 49
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 114
BfuI GTATCC 1 cut(s) 49
BisI GCNGC 2 cut(s) 315, 590
BlsI GCNGC 2 cut(s) 316, 591
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BpmI CTGGAG 1 cut(s) 283
BsaJI CCNNGG 2 cut(s) 211, 228
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bse3DI GCAATG 1 cut(s) 491
BseDI CCNNGG 2 cut(s) 211, 228
BseGI GGATG 2 cut(s) 13, 224
BseMI GCAATG 1 cut(s) 491
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 2 cut(s) 301, 601
Bsp19I CCATGG 1 cut(s) 211
BsrDI GCAATG 1 cut(s) 491
BssECI CCNNGG 2 cut(s) 211, 228
BssT1I CCWWGG 1 cut(s) 211
Bst4CI ACNGT 3 cut(s) 37, 323, 531
BstC8I GCNNGC 2 cut(s) 600, 626
BstDSI CCRYGG 2 cut(s) 211, 228
BstF5I GGATG 2 cut(s) 13, 224
BstMWI GCNNNNNNNGC 3 cut(s) 74, 287, 595
BstNSI RCATGY 1 cut(s) 281
BstSFI CTRYAG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 301, 601
BsuI GTATCC 1 cut(s) 49
BtgI CCRYGG 2 cut(s) 211, 228
BtsCI GGATG 2 cut(s) 13, 224
Cac8I GCNNGC 2 cut(s) 600, 626
Cfr13I GGNCC 1 cut(s) 329
CseI GACGC 1 cut(s) 196
CviAII CATG 5 cut(s) 157, 193, 212, 278, 371
CviJI RGCY 8 cut(s) 77, 164, 290, 341, 382, 433, 509, 614
CviKI_1 RGCY 8 cut(s) 77, 164, 290, 341, 382, 433, 509, 614
Eco130I CCWWGG 1 cut(s) 211
Eco47I GGWCC 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 211
FaeI CATG 5 cut(s) 160, 196, 215, 281, 374
FatI CATG 5 cut(s) 156, 192, 211, 277, 370
FblI GTMKAC 1 cut(s) 349
Fnu4HI GCNGC 2 cut(s) 315, 590
FokI GGATG 2 cut(s) 20, 211
Fsp4HI GCNGC 2 cut(s) 315, 590
FspBI CTAG 1 cut(s) 260
GluI GCNGC 2 cut(s) 315, 590
GsuI CTGGAG 1 cut(s) 283
HgaI GACGC 1 cut(s) 196
Hin1II CATG 5 cut(s) 160, 196, 215, 281, 374
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HindIII AAGCTT 1 cut(s) 162
HinfI GANTC 3 cut(s) 220, 254, 520
HpaI GTTAAC 1 cut(s) 538
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 2 cut(s) 350, 538
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 3 cut(s) 300, 407, 458
Hpy8I GTNNAC 2 cut(s) 350, 538
HpyAV CCTTC 2 cut(s) 128, 244
HpyCH4III ACNGT 3 cut(s) 37, 323, 531
HpyCH4IV ACGT 2 cut(s) 421, 472
HpyCH4V TGCA 3 cut(s) 68, 183, 239
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 287, 595
HpySE526I ACGT 2 cut(s) 421, 472
Hsp92II CATG 5 cut(s) 160, 196, 215, 281, 374
KspAI GTTAAC 1 cut(s) 538
LmnI GCTCC 3 cut(s) 287, 586, 595
LpnPI CCDG 8 cut(s) 157, 217, 313, 366, 376, 427, 578, 584
Lsp1109I GCAGC 2 cut(s) 301, 601
MaeI CTAG 1 cut(s) 260
MaeII ACGT 2 cut(s) 421, 472
MboII GAAGA 2 cut(s) 35, 584
MluCI AATT 7 cut(s) 45, 103, 269, 303, 357, 395, 446
MlyI GAGTC 1 cut(s) 229
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 5 cut(s) 43, 88, 240, 307, 577
MroXI GAANNNNTTC 2 cut(s) 307, 519
MseI TTAA 2 cut(s) 504, 537
MslI CAYNNNNRTG 2 cut(s) 554, 557
MwoI GCNNNNNNNGC 3 cut(s) 74, 287, 595
NcoI CCATGG 1 cut(s) 211
NlaIII CATG 5 cut(s) 160, 196, 215, 281, 374
NspI RCATGY 1 cut(s) 281
PdmI GAANNNNTTC 2 cut(s) 307, 519
PfeI GAWTC 2 cut(s) 254, 520
PkrI GCNGC 2 cut(s) 316, 591
PleI GAGTC 1 cut(s) 228
PpsI GAGTC 1 cut(s) 228
PspPI GGNCC 1 cut(s) 329
RseI CAYNNNNRTG 2 cut(s) 554, 557
SaqAI TTAA 2 cut(s) 504, 537
SatI GCNGC 2 cut(s) 315, 590
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 1 cut(s) 229
SetI ASST 8 cut(s) 166, 176, 292, 384, 424, 435, 475, 511
SfcI CTRYAG 1 cut(s) 114
SinI GGWCC 1 cut(s) 329
SmiMI CAYNNNNRTG 2 cut(s) 554, 557
Sse9I AATT 7 cut(s) 45, 103, 269, 303, 357, 395, 446
SspI AATATT 2 cut(s) 494, 545
SspMI CTAG 1 cut(s) 260
StyI CCWWGG 1 cut(s) 211
TaaI ACNGT 3 cut(s) 37, 323, 531
TaiI ACGT 2 cut(s) 424, 475
TasI AATT 7 cut(s) 45, 103, 269, 303, 357, 395, 446
TfiI GAWTC 2 cut(s) 254, 520
Tru1I TTAA 2 cut(s) 504, 537
Tru9I TTAA 2 cut(s) 504, 537
TseI GCWGC 2 cut(s) 314, 589
TspDTI ATGAA 4 cut(s) 36, 42, 173, 359
TspGWI ACGGA 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 329
XapI RAATTY 2 cut(s) 269, 303
XceI RCATGY 1 cut(s) 281
XmiI GTMKAC 1 cut(s) 349
XmnI GAANNNNTTC 2 cut(s) 307, 519
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.