Rh2CG293100

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002948
Physical Location & Seq
Forward (+)
33238609 .. 33244994
6386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG293100.1

Sequence Viewer

Length: 1077 bp
ATGATCATGGAGAGAAGAAGGAGATGGTTGCTGTTGTTGGCACTACTTACTTGCCTCTTTGCTTCTTCTGCTGGGAGAGAATGGAAGTTCAAGCACAAGGTTCATAGCCAGAGCCCTGTCTACAATCATACGCTTGCCACAATATTGGTTCGCTATGCCTCTACAGTGTATTTGTCAGATTTGACAGAGCTGTTTTCTTGGACATGCGACAGATGTGATGGCTTGATTAAGGATTTTCAAATGATAGAGATAATTGTGGACGTCCAGCATTGCTTACAGGCATTTGTTGGGGTGGCAACAGATCCAAATGCTATTATAATTGCATTTAAAGGGACTCAGGGAAACAGTATACAGAATTGGATCGAAGACTTATTTTGGAAACAACTTGATATAGATTACCCTGGCATGCCTGATGCAATGGTGCACCATGGTTTTTATAATGCTTACCACAACACAACCATACGACCTGGAATTCTAAATGCTGTTGCAAGAGCCAAAGAGTTTTATGGAGATATTGGCATCATAGTGACAGGGCATTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATTTAAGGGTTAATCAAAAGGAGAATAATGTTCAAGTTATGACATTTGGACAACCTCGAATCGGTAATGCAATTTTTGCTACTTACTATAGCGAAATTCTGCCACAGACTATACGAGTAACAAATGGAAATGATGTTGTGCCACATATGCCTCCATACTACACTTATTTTCCTCAGAAATACCACCACTTCCCAACAGAGGTGTGGCTTTATAACATTGGATTGCGAAGTCTTGTTTATCAGGTTGAGAAAATATGTGATGCCTCTGGTGAGGACCCAACCTGCAGCAGGTCAGTGATAGGGGACAGCATTTCAGACCATTTAGTTTATTATGGTGTCAAATTAATGGATGAGACATGGAGACCGTGCAAAATTGTGACGGGTCCTGGTTTACTGGATCATGGAAGATCAGATCTGGAAGGAAACTTTGTTCTGTCCAGAGATGTTGCAACTCCTGTTCTCAAATTGAAAATACAATCAGATGCTGGGGGAAAGCCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

358

Amino Acids

40.43

Weight (kDa)

6.49

Isoelectric Point (pI)

41.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 106 - 244 2.3e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 317, 438, 786
AatII GACGTC 1 cut(s) 264
Acc36I ACCTGC 2 cut(s) 852, 863
AccI GTMKAC 2 cut(s) 120, 349
AclWI GGATC 3 cut(s) 296, 368, 978
AcsI RAATTY 2 cut(s) 471, 669
AcyI GRCGYC 1 cut(s) 261
AfiI CCNNNNNNNGG 3 cut(s) 635, 772, 861
AgsI TTSAA 5 cut(s) 91, 239, 540, 608, 1042
AjnI CCWGG 3 cut(s) 400, 466, 958
AloI GAACNNNNNNTCC 2 cut(s) 987, 1019
AluBI AGCT 1 cut(s) 190
AluI AGCT 1 cut(s) 190
Alw21I GWGCWC 2 cut(s) 426, 571
Alw26I GTCTC 2 cut(s) 920, 928
Alw44I GTGCAC 1 cut(s) 422
AlwI GGATC 3 cut(s) 296, 368, 978
AlwNI CAGNNNCTG 1 cut(s) 1058
ApaLI GTGCAC 1 cut(s) 422
ApeKI GCWGC 1 cut(s) 858
ApoI RAATTY 2 cut(s) 471, 669
AseI ATTAAT 1 cut(s) 917
AspS9I GGNCC 2 cut(s) 847, 956
AsuHPI GGTGA 1 cut(s) 854
AvaII GGWCC 2 cut(s) 847, 956
BaeGI GKGCMC 1 cut(s) 426
BanII GRGCYC 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 372
Bbv12I GWGCWC 2 cut(s) 426, 571
BbvI GCAGC 1 cut(s) 870
BccI CCATC 2 cut(s) 18, 212
BciT130I CCWGG 3 cut(s) 402, 468, 960
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 2 cut(s) 920, 928
BfmI CTRYAG 4 cut(s) 162, 661, 856, 1073
BfuAI ACCTGC 2 cut(s) 852, 863
BglII AGATCT 1 cut(s) 985
BisI GCNGC 1 cut(s) 859
BlsI GCNGC 1 cut(s) 860
Bme1390I CCNGG 3 cut(s) 402, 468, 960
Bme18I GGWCC 2 cut(s) 847, 956
BmgT120I GGNCC 2 cut(s) 847, 956
BmiI GGNNCC 2 cut(s) 849, 957
BmrFI CCNGG 3 cut(s) 402, 468, 960
BmsI GCATC 4 cut(s) 403, 528, 823, 1045
BpiI GAAGAC 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 928
BsaJI CCNNGG 2 cut(s) 400, 427
Bsc4I CCNNNNNNNGG 3 cut(s) 635, 772, 861
Bse1I ACTGG 1 cut(s) 972
Bse3DI GCAATG 3 cut(s) 268, 423, 558
BseBI CCWGG 3 cut(s) 402, 468, 960
BseDI CCNNGG 2 cut(s) 400, 427
BseGI GGATG 1 cut(s) 928
BseLI CCNNNNNNNGG 3 cut(s) 635, 772, 861
BseMI GCAATG 3 cut(s) 268, 423, 558
BseMII CTCAG 2 cut(s) 350, 761
BseNI ACTGG 1 cut(s) 972
BseSI GKGCMC 1 cut(s) 426
BseXI GCAGC 1 cut(s) 870
BseYI CCCAGC 2 cut(s) 71, 1058
BsiHKAI GWGCWC 2 cut(s) 426, 571
BslFI GGGAC 2 cut(s) 346, 890
BslI CCNNNNNNNGG 3 cut(s) 635, 772, 861
BsmAI GTCTC 2 cut(s) 920, 928
BsmFI GGGAC 2 cut(s) 346, 890
BsmI GAATGC 1 cut(s) 535
Bso31I GGTCTC 1 cut(s) 928
Bsp1286I GDGCHC 3 cut(s) 116, 426, 571
Bsp143I GATC 6 cut(s) 3, 301, 360, 970, 980, 985
Bsp19I CCATGG 1 cut(s) 427
BspCNI CTCAG 2 cut(s) 349, 760
BspLI GGNNCC 2 cut(s) 849, 957
BspMAI CTGCAG 1 cut(s) 860
BspMI ACCTGC 2 cut(s) 852, 863
BspPI GGATC 3 cut(s) 296, 368, 978
BspTNI GGTCTC 1 cut(s) 928
BsrDI GCAATG 3 cut(s) 268, 423, 558
BsrI ACTGG 1 cut(s) 972
BssECI CCNNGG 2 cut(s) 400, 427
BssMI GATC 6 cut(s) 3, 301, 360, 970, 980, 985
BssNAI GTATAC 1 cut(s) 350
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 427
Bst1107I GTATAC 1 cut(s) 350
Bst2UI CCWGG 3 cut(s) 402, 468, 960
Bst4CI ACNGT 3 cut(s) 166, 347, 939
BstACI GRCGYC 1 cut(s) 261
BstAPI GCANNNNNTGC 1 cut(s) 650
BstC8I GCNNGC 2 cut(s) 135, 407
BstDEI CTNAG 2 cut(s) 336, 747
BstDSI CCRYGG 1 cut(s) 427
BstENI CCTNNNNNAGG 1 cut(s) 859
BstF5I GGATG 1 cut(s) 928
BstKTI GATC 6 cut(s) 6, 304, 363, 973, 983, 988
BstMAI GTCTC 2 cut(s) 920, 928
BstMBI GATC 6 cut(s) 3, 301, 360, 970, 980, 985
BstMWI GCNNNNNNNGC 3 cut(s) 68, 650, 721
BstNI CCWGG 3 cut(s) 402, 468, 960
BstNSI RCATGY 2 cut(s) 207, 409
BstSCI CCNGG 3 cut(s) 400, 466, 958
BstSFI CTRYAG 4 cut(s) 162, 661, 856, 1073
BstSLI GKGCMC 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 870
BstV2I GAAGAC 1 cut(s) 372
BstX2I RGATCY 2 cut(s) 301, 985
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 2 cut(s) 301, 985
BstZ17I GTATAC 1 cut(s) 350
BtgI CCRYGG 1 cut(s) 427
BtsCI GGATG 1 cut(s) 928
BtsIMutI CAGTG 2 cut(s) 171, 873
BveI ACCTGC 2 cut(s) 852, 863
Cac8I GCNNGC 2 cut(s) 135, 407
CaiI CAGNNNCTG 1 cut(s) 1058
Cfr13I GGNCC 2 cut(s) 847, 956
CviAII CATG 6 cut(s) 7, 204, 406, 428, 930, 974
CviJI RGCY 8 cut(s) 108, 114, 190, 222, 494, 557, 781, 1069
CviKI_1 RGCY 8 cut(s) 108, 114, 190, 222, 494, 557, 781, 1069
DdeI CTNAG 2 cut(s) 336, 747
DpnI GATC 6 cut(s) 5, 303, 362, 972, 982, 987
DpnII GATC 6 cut(s) 3, 301, 360, 970, 980, 985
DraI TTTAAA 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 427
Eco24I GRGCYC 1 cut(s) 116
Eco31I GGTCTC 1 cut(s) 928
Eco47I GGWCC 2 cut(s) 847, 956
EcoNI CCTNNNNNAGG 1 cut(s) 859
EcoO109I RGGNCCY 2 cut(s) 847, 956
EcoRI GAATTC 1 cut(s) 471
EcoRII CCWGG 3 cut(s) 400, 466, 958
EcoT14I CCWWGG 1 cut(s) 427
EcoT38I GRGCYC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 6 cut(s) 10, 207, 409, 431, 933, 977
FaqI GGGAC 2 cut(s) 346, 890
FatI CATG 6 cut(s) 6, 203, 405, 427, 929, 973
FauNDI CATATG 1 cut(s) 720
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 120, 349
Fnu4HI GCNGC 1 cut(s) 859
FokI GGATG 1 cut(s) 935
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 1 cut(s) 859
GluI GCNGC 1 cut(s) 859
GsaI CCCAGC 2 cut(s) 75, 1062
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 6 cut(s) 10, 207, 409, 431, 933, 977
HinfI GANTC 2 cut(s) 334, 633
HphI GGTGA 1 cut(s) 854
Hpy166II GTNNAC 5 cut(s) 121, 259, 350, 424, 965
Hpy188I TCNGA 5 cut(s) 178, 750, 889, 985, 1054
Hpy188III TCNNGA 3 cut(s) 572, 989, 1011
Hpy8I GTNNAC 5 cut(s) 121, 259, 350, 424, 965
HpyAV CCTTC 2 cut(s) 12, 986
HpyCH4III ACNGT 3 cut(s) 166, 347, 939
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 8 cut(s) 323, 416, 424, 488, 644, 858, 942, 1022
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 650, 721
HpyF3I CTNAG 2 cut(s) 336, 747
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 6 cut(s) 10, 207, 409, 431, 933, 977
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 301, 360, 970, 980, 985
Lsp1109I GCAGC 1 cut(s) 870
LweI GCATC 4 cut(s) 403, 528, 823, 1045
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 526, 691, 949
MalI GATC 6 cut(s) 5, 303, 362, 972, 982, 987
MboI GATC 6 cut(s) 3, 301, 360, 970, 980, 985
MboII GAAGA 4 cut(s) 27, 57, 377, 990
MflI RGATCY 2 cut(s) 301, 985
MhlI GDGCHC 3 cut(s) 116, 426, 571
MluCI AATT 9 cut(s) 252, 318, 355, 471, 645, 669, 914, 945, 1037
MlyI GAGTC 1 cut(s) 328
MnlI CCTC 9 cut(s) 65, 169, 539, 639, 735, 756, 766, 838, 847
MseI TTAA 5 cut(s) 228, 327, 578, 585, 917
MslI CAYNNNNRTG 1 cut(s) 524
MspR9I CCNGG 3 cut(s) 402, 468, 960
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 3 cut(s) 402, 468, 960
MwoI GCNNNNNNNGC 3 cut(s) 68, 650, 721
NcoI CCATGG 1 cut(s) 427
NdeI CATATG 1 cut(s) 720
NdeII GATC 6 cut(s) 3, 301, 360, 970, 980, 985
NlaIII CATG 6 cut(s) 10, 207, 409, 431, 933, 977
NlaIV GGNNCC 2 cut(s) 849, 957
NmuCI GTSAC 2 cut(s) 526, 949
NspI RCATGY 2 cut(s) 207, 409
PaeI GCATGC 1 cut(s) 409
PctI GAATGC 1 cut(s) 535
PfeI GAWTC 1 cut(s) 633
PkrI GCNGC 1 cut(s) 860
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
PpuMI RGGWCCY 2 cut(s) 847, 956
PshBI ATTAAT 1 cut(s) 917
PsiI TTATAA 3 cut(s) 317, 438, 786
Psp5II RGGWCCY 2 cut(s) 847, 956
Psp6I CCWGG 3 cut(s) 400, 466, 958
PspFI CCCAGC 2 cut(s) 71, 1058
PspGI CCWGG 3 cut(s) 400, 466, 958
PspN4I GGNNCC 2 cut(s) 849, 957
PspPI GGNCC 2 cut(s) 847, 956
PspPPI RGGWCCY 2 cut(s) 847, 956
PstI CTGCAG 1 cut(s) 860
PstNI CAGNNNCTG 1 cut(s) 1058
PsuI RGATCY 2 cut(s) 301, 985
RseI CAYNNNNRTG 1 cut(s) 524
SaqAI TTAA 5 cut(s) 228, 327, 578, 585, 917
SatI GCNGC 1 cut(s) 859
Sau3AI GATC 6 cut(s) 3, 301, 360, 970, 980, 985
Sau96I GGNCC 2 cut(s) 847, 956
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 3 cut(s) 402, 468, 960
SduI GDGCHC 3 cut(s) 116, 426, 571
SetI ASST 9 cut(s) 102, 192, 264, 469, 631, 777, 819, 857, 866
SfaNI GCATC 4 cut(s) 403, 528, 823, 1045
SfcI CTRYAG 4 cut(s) 162, 661, 856, 1073
SinI GGWCC 2 cut(s) 847, 956
SmiMI CAYNNNNRTG 1 cut(s) 524
SphI GCATGC 1 cut(s) 409
Sse9I AATT 9 cut(s) 252, 318, 355, 471, 645, 669, 914, 945, 1037
SspI AATATT 1 cut(s) 144
StyD4I CCNGG 3 cut(s) 400, 466, 958
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 3 cut(s) 166, 347, 939
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 363, 631
TasI AATT 9 cut(s) 252, 318, 355, 471, 645, 669, 914, 945, 1037
TfiI GAWTC 1 cut(s) 633
Tru1I TTAA 5 cut(s) 228, 327, 578, 585, 917
Tru9I TTAA 5 cut(s) 228, 327, 578, 585, 917
TscAI CASTG 2 cut(s) 171, 873
TseFI GTSAC 2 cut(s) 526, 949
TseI GCWGC 1 cut(s) 858
Tsp45I GTSAC 2 cut(s) 526, 949
TspDTI ATGAA 2 cut(s) 92, 549
TspRI CASTG 2 cut(s) 171, 873
VneI GTGCAC 1 cut(s) 422
VpaK11BI GGWCC 2 cut(s) 847, 956
VspI ATTAAT 1 cut(s) 917
XagI CCTNNNNNAGG 1 cut(s) 859
XapI RAATTY 2 cut(s) 471, 669
XceI RCATGY 2 cut(s) 207, 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 774
XmiI GTMKAC 2 cut(s) 120, 349
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.