AT5G18640

lipase class 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002948
Physical Location & Seq
Forward (+)
6212773 .. 6215863
3091 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18640.5

Sequence Viewer

Length: 1074 bp
ATGGGACAAAAGAGATGGTTCTTCTTGTTGGCAATTTTTGTTTGTCTTGTTTCATCTTCATGTGGTAGAGTTCTCAAGTTTAAGAGCGATGATGGTCGACCTGTGTACAATCATACGCTTGCCATCACACTTGTGGAGTATACTTCTGCGGTCTATATGTCTGATTTGTCAGAACTCTTCACTTGGACATGCGAAAGATGCAATGGTTTGACAAAGGGTTTTGAGGTAATAGAGATAATAGTTGACGTTGAGCACTGCTTACAGGCATATGTTGGGGTGGCTAAGGATTTGAATGCTATCATTATTGCATTTCGGGGAACTCAAGAACACAGCATACAAAATTGGGTCTCAGACTTGTTCTGGAAACAGCTCGATCTAAATTACCCTGATATGCCAGATGCAATGGTGCACCATGGCTTTTATAGTGCTTATCACAATACAACTGTACGGCCTGCAGTTTTGGATGCAGTAAAACGAGCAAAGGAATCCTATGGGGCCAACCTCAACATCATGGTGACTGGTCATTCAATGGGAGGAGCCATGGCTTCCTTTTGTGCGCTAGACCTAGTTGTTAATGAAGGAGAAGAAAACGTACAAGTTATGACATTTGGGCAACCTCGTGTTGGGAATGCTGCTTTTGCATCTTATTTCAATTTGCTTGTGCCTAATACCTTCCGGATCATACATGACCGTGACATAGTTCCTCATCTGCCTCCTTACTATCATCTTTTCCCTCAAAAAACATATCACCACTTCCCCACAGAGGTGTGGTTGACAGAACTAAGTATTTTGAATATTGTTATCCGTGGTGTGGAAAAAGTTTGTGACAACACCGGTGAAGATCCTACATGCAGCAGATCGGTAATGGGAAATAGCATATCTGACCATTTAACGTACTTTGGAGTGGAGTTGATGGGTGAGACTTGGAGGCAATGTAACATAGTGATGGGCCATGAGATGGAGAGTTACAGTAGGAAAGATTCAAAGGGTAACATATTCCTTTCGCGGACAGTTCCTTCCACGGAGGTAATCAAAACAAAAAGTATATCCAAAACCGGAATCTCAAGTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

357

Amino Acids

40.23

Weight (kDa)

6.05

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 101 - 240 3.2e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 958
AccI GTMKAC 2 cut(s) 97, 140
AccII CGCG 1 cut(s) 1006
AccIII TCCGGA 1 cut(s) 675
AciI CCGC 2 cut(s) 149, 1006
AclWI GGATC 2 cut(s) 686, 836
AfaI GTAC 4 cut(s) 107, 447, 594, 896
AfiI CCNNNNNNNGG 4 cut(s) 623, 763, 811, 958
AgeI ACCGGT 1 cut(s) 833
AgsI TTSAA 5 cut(s) 292, 528, 652, 793, 984
AleI CACNNNNGTG 2 cut(s) 131, 764
AluBI AGCT 1 cut(s) 370
AluI AGCT 1 cut(s) 370
Alw21I GWGCWC 2 cut(s) 255, 411
Alw26I GTCTC 2 cut(s) 352, 914
Alw44I GTGCAC 1 cut(s) 407
AlwI GGATC 2 cut(s) 686, 836
Aor13HI TCCGGA 1 cut(s) 675
AoxI GGCC 3 cut(s) 449, 495, 949
ApaLI GTGCAC 1 cut(s) 407
ApeKI GCWGC 2 cut(s) 632, 852
AsiGI ACCGGT 1 cut(s) 833
AspLEI GCGC 1 cut(s) 559
AspS9I GGNCC 2 cut(s) 495, 949
AsuHPI GGTGA 4 cut(s) 526, 740, 848, 929
BaeGI GKGCMC 1 cut(s) 411
BarI GAAGNNNNNNTAC 2 cut(s) 576, 608
BauI CACGAG 1 cut(s) 618
Bbv12I GWGCWC 2 cut(s) 255, 411
BbvI GCAGC 2 cut(s) 619, 864
BccI CCATC 6 cut(s) 9, 86, 131, 907, 940, 952
BceAI ACGGC 1 cut(s) 464
BcoDI GTCTC 2 cut(s) 352, 914
BfaI CTAG 2 cut(s) 560, 566
BfmI CTRYAG 2 cut(s) 453, 1070
BisI GCNGC 2 cut(s) 633, 853
BlsI GCNGC 2 cut(s) 634, 854
BmgT120I GGNCC 2 cut(s) 495, 949
BmiI GGNNCC 2 cut(s) 496, 538
BmsI GCATC 4 cut(s) 188, 388, 454, 650
Bpu10I CCTNAGC 1 cut(s) 282
BpuEI CTTGAG 3 cut(s) 59, 306, 1048
BsaI GGTCTC 1 cut(s) 352
BsaJI CCNNGG 4 cut(s) 412, 540, 805, 1020
BsaWI WCCGGW 3 cut(s) 675, 833, 1055
BsaXI ACNNNNNCTCC 2 cut(s) 919, 949
Bsc4I CCNNNNNNNGG 4 cut(s) 623, 763, 811, 958
Bse118I RCCGGY 1 cut(s) 833
Bse1I ACTGG 1 cut(s) 523
Bse3DI GCAATG 3 cut(s) 208, 408, 938
BseAI TCCGGA 1 cut(s) 675
BseDI CCNNGG 4 cut(s) 412, 540, 805, 1020
BseGI GGATG 1 cut(s) 469
BseLI CCNNNNNNNGG 4 cut(s) 623, 763, 811, 958
BseMI GCAATG 3 cut(s) 208, 408, 938
BseMII CTCAG 1 cut(s) 363
BseNI ACTGG 1 cut(s) 523
BseRI GAGGAG 1 cut(s) 549
BseSI GKGCMC 1 cut(s) 411
BseXI GCAGC 2 cut(s) 619, 864
Bsh1236I CGCG 1 cut(s) 1006
BshFI GGCC 3 cut(s) 451, 497, 951
BshTI ACCGGT 1 cut(s) 833
BsiHKAI GWGCWC 2 cut(s) 255, 411
BsiSI CCGG 3 cut(s) 676, 834, 1056
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 4 cut(s) 623, 763, 811, 958
BsmAI GTCTC 2 cut(s) 352, 914
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 2 cut(s) 298, 634
BsnI GGCC 3 cut(s) 451, 497, 951
Bso31I GGTCTC 1 cut(s) 352
Bsp1286I GDGCHC 2 cut(s) 255, 411
Bsp13I TCCGGA 1 cut(s) 675
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 4 cut(s) 373, 678, 841, 857
Bsp19I CCATGG 2 cut(s) 412, 540
BspACI CCGC 2 cut(s) 149, 1006
BspANI GGCC 3 cut(s) 451, 497, 951
BspCNI CTCAG 1 cut(s) 362
BspEI TCCGGA 1 cut(s) 675
BspFNI CGCG 1 cut(s) 1006
BspLI GGNNCC 2 cut(s) 496, 538
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 2 cut(s) 686, 836
BspTNI GGTCTC 1 cut(s) 352
BsrDI GCAATG 3 cut(s) 208, 408, 938
BsrFI RCCGGY 1 cut(s) 833
BsrGI TGTACA 1 cut(s) 105
BsrI ACTGG 1 cut(s) 523
BssAI RCCGGY 1 cut(s) 833
BssECI CCNNGG 4 cut(s) 412, 540, 805, 1020
BssMI GATC 4 cut(s) 373, 678, 841, 857
BssNAI GTATAC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 618
BssT1I CCWWGG 2 cut(s) 412, 540
Bst1107I GTATAC 1 cut(s) 141
Bst2BI CACGAG 1 cut(s) 618
Bst4CI ACNGT 4 cut(s) 445, 692, 971, 1012
Bst6I CTCTTC 1 cut(s) 182
BstAUI TGTACA 1 cut(s) 105
BstC8I GCNNGC 2 cut(s) 120, 453
BstDEI CTNAG 3 cut(s) 282, 349, 782
BstDSI CCRYGG 4 cut(s) 412, 540, 805, 1020
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 1 cut(s) 1006
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 4 cut(s) 376, 681, 844, 860
BstMAI GTCTC 2 cut(s) 352, 914
BstMBI GATC 4 cut(s) 373, 678, 841, 857
BstMWI GCNNNNNNNGC 2 cut(s) 198, 638
BstNSI RCATGY 2 cut(s) 192, 852
BstSFI CTRYAG 2 cut(s) 453, 1070
BstSLI GKGCMC 1 cut(s) 411
BstUI CGCG 1 cut(s) 1006
BstV1I GCAGC 2 cut(s) 619, 864
BstX2I RGATCY 1 cut(s) 841
BstYI RGATCY 1 cut(s) 841
BstZ17I GTATAC 1 cut(s) 141
BsuRI GGCC 3 cut(s) 451, 497, 951
BtgI CCRYGG 4 cut(s) 412, 540, 805, 1020
BtgZI GCGATG 1 cut(s) 102
BtsCI GGATG 1 cut(s) 469
BtsI GCAGTG 1 cut(s) 253
BtsIMutI CAGTG 1 cut(s) 253
Cac8I GCNNGC 2 cut(s) 120, 453
CfoI GCGC 1 cut(s) 559
Cfr10I RCCGGY 1 cut(s) 833
Cfr13I GGNCC 2 cut(s) 495, 949
Csp6I GTAC 4 cut(s) 106, 446, 593, 895
CspAI ACCGGT 1 cut(s) 833
CviAII CATG 8 cut(s) 60, 189, 413, 511, 541, 686, 849, 953
CviJI RGCY 8 cut(s) 281, 370, 417, 451, 497, 539, 545, 951
CviKI_1 RGCY 8 cut(s) 281, 370, 417, 451, 497, 539, 545, 951
CviQI GTAC 4 cut(s) 106, 446, 593, 895
DdeI CTNAG 3 cut(s) 282, 349, 782
DpnI GATC 4 cut(s) 375, 680, 843, 859
DpnII GATC 4 cut(s) 373, 678, 841, 857
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Eco130I CCWWGG 2 cut(s) 412, 540
Eco31I GGTCTC 1 cut(s) 352
EcoT14I CCWWGG 2 cut(s) 412, 540
ErhI CCWWGG 2 cut(s) 412, 540
FaeI CATG 8 cut(s) 63, 192, 416, 514, 544, 689, 852, 956
FaqI GGGAC 1 cut(s) 18
FatI CATG 8 cut(s) 59, 188, 412, 510, 540, 685, 848, 952
FauNDI CATATG 1 cut(s) 268
FblI GTMKAC 2 cut(s) 97, 140
Fnu4HI GCNGC 2 cut(s) 633, 853
FokI GGATG 1 cut(s) 476
Fsp4HI GCNGC 2 cut(s) 633, 853
FspBI CTAG 2 cut(s) 560, 566
GlaI GCGC 1 cut(s) 558
GluI GCNGC 2 cut(s) 633, 853
HaeIII GGCC 3 cut(s) 451, 497, 951
HapII CCGG 3 cut(s) 676, 834, 1056
HhaI GCGC 1 cut(s) 559
Hin1II CATG 8 cut(s) 63, 192, 416, 514, 544, 689, 852, 956
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HincII GTYRAC 3 cut(s) 98, 244, 774
HindII GTYRAC 3 cut(s) 98, 244, 774
HinfI GANTC 3 cut(s) 485, 980, 1059
HpaII CCGG 3 cut(s) 676, 834, 1056
HphI GGTGA 4 cut(s) 526, 740, 848, 929
Hpy166II GTNNAC 6 cut(s) 98, 106, 141, 244, 409, 774
Hpy188I TCNGA 4 cut(s) 163, 172, 352, 883
Hpy188III TCNNGA 3 cut(s) 323, 361, 676
Hpy8I GTNNAC 6 cut(s) 98, 106, 141, 244, 409, 774
HpyAV CCTTC 3 cut(s) 572, 682, 1026
HpyCH4III ACNGT 4 cut(s) 445, 692, 971, 1012
HpyCH4IV ACGT 3 cut(s) 246, 591, 893
HpyCH4V TGCA 8 cut(s) 201, 308, 401, 409, 455, 467, 641, 852
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 638
HpyF3I CTNAG 3 cut(s) 282, 349, 782
HpySE526I ACGT 3 cut(s) 246, 591, 893
Hsp92II CATG 8 cut(s) 63, 192, 416, 514, 544, 689, 852, 956
HspAI GCGC 1 cut(s) 557
Kpn2I TCCGGA 1 cut(s) 675
Kzo9I GATC 4 cut(s) 373, 678, 841, 857
LmnI GCTCC 1 cut(s) 536
Lsp1109I GCAGC 2 cut(s) 619, 864
LweI GCATC 4 cut(s) 188, 388, 454, 650
MaeI CTAG 2 cut(s) 560, 566
MaeII ACGT 3 cut(s) 246, 591, 893
MaeIII GTNAC 6 cut(s) 514, 692, 824, 935, 965, 989
MalI GATC 4 cut(s) 375, 680, 843, 859
MboI GATC 4 cut(s) 373, 678, 841, 857
MboII GAAGA 5 cut(s) 13, 48, 169, 596, 851
MflI RGATCY 1 cut(s) 841
MhlI GDGCHC 2 cut(s) 255, 411
MluCI AATT 4 cut(s) 33, 340, 379, 652
MroI TCCGGA 1 cut(s) 675
MseI TTAA 3 cut(s) 81, 573, 890
MslI CAYNNNNRTG 6 cut(s) 58, 131, 512, 690, 764, 944
MspI CCGG 3 cut(s) 676, 834, 1056
Mva1269I GAATGC 2 cut(s) 298, 634
MvnI CGCG 1 cut(s) 1006
MwoI GCNNNNNNNGC 2 cut(s) 198, 638
NcoI CCATGG 2 cut(s) 412, 540
NdeI CATATG 1 cut(s) 268
NdeII GATC 4 cut(s) 373, 678, 841, 857
NlaIII CATG 8 cut(s) 63, 192, 416, 514, 544, 689, 852, 956
NlaIV GGNNCC 2 cut(s) 496, 538
NmuCI GTSAC 3 cut(s) 514, 692, 824
NspI RCATGY 2 cut(s) 192, 852
OliI CACNNNNGTG 2 cut(s) 131, 764
PctI GAATGC 2 cut(s) 298, 634
PfeI GAWTC 3 cut(s) 485, 980, 1059
PflMI CCANNNNNTGG 1 cut(s) 958
PinAI ACCGGT 1 cut(s) 833
PkrI GCNGC 2 cut(s) 634, 854
PspN4I GGNNCC 2 cut(s) 496, 538
PspPI GGNCC 2 cut(s) 495, 949
PsrI GAACNNNNNNTAC 2 cut(s) 318, 350
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 841
RsaI GTAC 4 cut(s) 107, 447, 594, 896
RsaNI GTAC 4 cut(s) 106, 446, 593, 895
RseI CAYNNNNRTG 6 cut(s) 58, 131, 512, 690, 764, 944
SalI GTCGAC 1 cut(s) 96
SaqAI TTAA 3 cut(s) 81, 573, 890
SatI GCNGC 2 cut(s) 633, 853
Sau3AI GATC 4 cut(s) 373, 678, 841, 857
Sau96I GGNCC 2 cut(s) 495, 949
SduI GDGCHC 2 cut(s) 255, 411
SfaNI GCATC 4 cut(s) 188, 388, 454, 650
SfcI CTRYAG 2 cut(s) 453, 1070
SgrAI CRCCGGYG 1 cut(s) 833
SmiMI CAYNNNNRTG 6 cut(s) 58, 131, 512, 690, 764, 944
SmlI CTYRAG 3 cut(s) 74, 321, 1063
SmoI CTYRAG 3 cut(s) 74, 321, 1063
Sse9I AATT 4 cut(s) 33, 340, 379, 652
SsiI CCGC 2 cut(s) 149, 1006
SspI AATATT 1 cut(s) 796
SspMI CTAG 2 cut(s) 560, 566
StyI CCWWGG 2 cut(s) 412, 540
TaaI ACNGT 4 cut(s) 445, 692, 971, 1012
TaiI ACGT 3 cut(s) 249, 594, 896
TaqI TCGA 2 cut(s) 97, 372
TasI AATT 4 cut(s) 33, 340, 379, 652
TatI WGTACW 1 cut(s) 105
TfiI GAWTC 3 cut(s) 485, 980, 1059
Tru1I TTAA 3 cut(s) 81, 573, 890
Tru9I TTAA 3 cut(s) 81, 573, 890
TscAI CASTG 1 cut(s) 260
TseFI GTSAC 3 cut(s) 514, 692, 824
TseI GCWGC 2 cut(s) 632, 852
Tsp45I GTSAC 3 cut(s) 514, 692, 824
TspDTI ATGAA 3 cut(s) 42, 48, 591
TspGWI ACGGA 2 cut(s) 794, 1037
TspRI CASTG 1 cut(s) 260
Van91I CCANNNNNTGG 1 cut(s) 958
VneI GTGCAC 1 cut(s) 407
XceI RCATGY 2 cut(s) 192, 852
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XmiI GTMKAC 2 cut(s) 97, 140
XspI CTAG 2 cut(s) 560, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.