FvH4_4g17380

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002948
Physical Location & Seq
Reverse (-)
21296103 .. 21301553
5451 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17380.t1

Sequence Viewer

Length: 1071 bp
ATGGAGAGAAGGAGATGGTTGGTATTGCTGGCAGTACTGGCCTGTTTGGTTGTTTGCTCAGCTGGGAGAGAGCTGAGATTCAAGCACAAGTTTCATGGACATAGCCCCGTCTATAATCATACGCTTGCTACAATATTGGTTCGGTATGCTTCAACGGTGTATGTGTCAGATTTGACAGAGCTGTTTTCTTGGACATGCAAAAATTGTAATGGCTTGATCAAGGATTTTCAAATGATAGAGCTGATTGTTGACATCCAGCATTGCCTACAGGCATTTGTTGGAGTGGCAACAGATCCTAATGCTATTATAATTGCCTTTAGAGGGACTCAGGAACACAGTATACAGAATTGGATTGAAGACTTATTTTGGAAACAACTTGATATAGATTACCCCGGCATGCCTGATTCAAGGGTGCACCATGGGTTTTATAGCGCTTACCACAACACAACCATACGTCCTGGAATTCTAAATGCTGTTGCAAGAGCTAAAGAGTTTTATGGAGATATTGGCATCATAGTGACAGGGCATTCAATGGGAGGGGCGATGGCTTCATTTTGTGCTCTTGATTTAAGGGTCAATCAAAAGGAGAAGAATGTTCAAGTTATGACATTTGGACAACCTCGTATTGGTAATGCAGTCTTTGCTACTTTCTATAGCAAACTTGTGCCAGACACTATACGGATAACAAATGGAAATGATGTTGTCCCTCATTTGCCTCCATACTATACTTACTTCCCTCAGAAGACATACCACCACTTCCCAAGAGAGGTATGGCTTTATAACATTGGAATGGGAAGTCTTGTTTATAAGGTTGAGAAAATCTGTGATTCATCTGGTGAGGACCCAACTTGCAGCAGGTCAGTGAAAGGGAATAGTATTTCAGACCATTTAGTTTATTTTGGTGTGGATTTAATGGCCAAGACATGGAGGCCGTGCAAAATTGTGATGGGTCCTGGTTTACTGGAGCATGGCAGAACAGATCTGGAAGGAAATTTTGTACTGTCCAGAGATCTTTCAACTCCTGGTCTCAAATTGAAAACACGGCCAGATGCCAGAGGAAAGCCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

357

Amino Acids

40.21

Weight (kDa)

8.75

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 103 - 241 1.6e-36 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 308, 780, 807
Acc36I ACCTGC 1 cut(s) 846
AccB7I CCANNNNNTGG 1 cut(s) 924
AccI GTMKAC 1 cut(s) 340
AclWI GGATC 1 cut(s) 287
AcoI YGGCCR 2 cut(s) 915, 1043
AcsI RAATTY 2 cut(s) 462, 991
AfaI GTAC 2 cut(s) 36, 999
AfeI AGCGCT 1 cut(s) 433
AfiI CCNNNNNNNGG 4 cut(s) 321, 626, 766, 924
AgsI TTSAA 9 cut(s) 82, 153, 230, 356, 408, 531, 599, 1017, 1036
AjnI CCWGG 3 cut(s) 457, 952, 1021
AluBI AGCT 5 cut(s) 62, 73, 181, 241, 485
AluI AGCT 5 cut(s) 62, 73, 181, 241, 485
Alw21I GWGCWC 2 cut(s) 417, 562
Alw26I GTCTC 1 cut(s) 1031
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 1 cut(s) 287
Aor51HI AGCGCT 1 cut(s) 433
AoxI GGCC 4 cut(s) 39, 915, 929, 1043
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 1 cut(s) 852
ApoI RAATTY 2 cut(s) 462, 991
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 2 cut(s) 841, 950
AsuC2I CCSGG 1 cut(s) 393
AsuHPI GGTGA 1 cut(s) 848
AvaII GGWCC 2 cut(s) 841, 950
BaeGI GKGCMC 1 cut(s) 417
BalI TGGCCA 1 cut(s) 917
BbsI GAAGAC 2 cut(s) 363, 749
Bbv12I GWGCWC 2 cut(s) 417, 562
BbvI GCAGC 1 cut(s) 864
BccI CCATC 3 cut(s) 9, 538, 940
BceAI ACGGC 2 cut(s) 916, 1058
BciT130I CCWGG 3 cut(s) 459, 954, 1023
BclI TGATCA 1 cut(s) 216
BcnI CCSGG 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 1031
BfmI CTRYAG 3 cut(s) 266, 652, 1067
BfoI RGCGCY 1 cut(s) 435
BfuAI ACCTGC 1 cut(s) 846
BglII AGATCT 2 cut(s) 979, 1009
BisI GCNGC 1 cut(s) 853
BlpI GCTNAGC 1 cut(s) 58
BlsI GCNGC 1 cut(s) 854
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 4 cut(s) 393, 459, 954, 1023
Bme18I GGWCC 2 cut(s) 841, 950
BmgT120I GGNCC 2 cut(s) 841, 950
BmiI GGNNCC 2 cut(s) 843, 951
BmrFI CCNGG 4 cut(s) 393, 459, 954, 1023
BmsI GCATC 2 cut(s) 519, 1039
BpiI GAAGAC 2 cut(s) 363, 749
BpmI CTGGAG 1 cut(s) 983
Bpu1102I GCTNAGC 1 cut(s) 58
BpuMI CCSGG 1 cut(s) 393
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 2 cut(s) 391, 418
Bsc4I CCNNNNNNNGG 4 cut(s) 321, 626, 766, 924
Bse1I ACTGG 2 cut(s) 42, 966
Bse3DI GCAATG 1 cut(s) 259
BseBI CCWGG 3 cut(s) 459, 954, 1023
BseDI CCNNGG 2 cut(s) 391, 418
BseGI GGATG 1 cut(s) 252
BseLI CCNNNNNNNGG 4 cut(s) 321, 626, 766, 924
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 4 cut(s) 65, 72, 341, 752
BseNI ACTGG 2 cut(s) 42, 966
BseSI GKGCMC 1 cut(s) 417
BseXI GCAGC 1 cut(s) 864
BseYI CCCAGC 1 cut(s) 62
BshFI GGCC 4 cut(s) 41, 917, 931, 1045
BsiHKAI GWGCWC 2 cut(s) 417, 562
BsiSI CCGG 1 cut(s) 393
BslFI GGGAC 2 cut(s) 337, 689
BslI CCNNNNNNNGG 4 cut(s) 321, 626, 766, 924
BsmAI GTCTC 1 cut(s) 1031
BsmFI GGGAC 2 cut(s) 337, 689
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 4 cut(s) 41, 917, 931, 1045
Bso31I GGTCTC 1 cut(s) 1031
Bsp1286I GDGCHC 2 cut(s) 417, 562
Bsp143I GATC 4 cut(s) 216, 292, 979, 1009
Bsp1720I GCTNAGC 1 cut(s) 58
Bsp19I CCATGG 1 cut(s) 418
BspANI GGCC 4 cut(s) 41, 917, 931, 1045
BspCNI CTCAG 4 cut(s) 66, 71, 340, 751
BspLI GGNNCC 2 cut(s) 843, 951
BspMI ACCTGC 1 cut(s) 846
BspPI GGATC 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 1031
BsrDI GCAATG 1 cut(s) 259
BsrI ACTGG 2 cut(s) 42, 966
BssECI CCNNGG 2 cut(s) 391, 418
BssMI GATC 4 cut(s) 216, 292, 979, 1009
BssNAI GTATAC 1 cut(s) 341
BssT1I CCWWGG 1 cut(s) 418
Bst1107I GTATAC 1 cut(s) 341
Bst2UI CCWGG 3 cut(s) 459, 954, 1023
Bst4CI ACNGT 3 cut(s) 157, 338, 1002
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 3 cut(s) 30, 126, 398
BstDEI CTNAG 4 cut(s) 58, 74, 327, 738
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 1 cut(s) 252
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 4 cut(s) 219, 295, 982, 1012
BstMAI GTCTC 1 cut(s) 1031
BstMBI GATC 4 cut(s) 216, 292, 979, 1009
BstMWI GCNNNNNNNGC 2 cut(s) 38, 641
BstNI CCWGG 3 cut(s) 459, 954, 1023
BstNSI RCATGY 2 cut(s) 198, 400
BstSCI CCNGG 4 cut(s) 391, 457, 952, 1021
BstSFI CTRYAG 3 cut(s) 266, 652, 1067
BstSLI GKGCMC 1 cut(s) 417
BstV1I GCAGC 1 cut(s) 864
BstV2I GAAGAC 2 cut(s) 363, 749
BstX2I RGATCY 3 cut(s) 292, 979, 1009
BstYI RGATCY 3 cut(s) 292, 979, 1009
BstZ17I GTATAC 1 cut(s) 341
BsuRI GGCC 4 cut(s) 41, 917, 931, 1045
BtgI CCRYGG 1 cut(s) 418
BtgZI GCGATG 1 cut(s) 557
BtsCI GGATG 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 867
BveI ACCTGC 1 cut(s) 846
Cac8I GCNNGC 3 cut(s) 30, 126, 398
CfoI GCGC 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 841, 950
Csp6I GTAC 2 cut(s) 35, 998
CviAII CATG 6 cut(s) 95, 195, 397, 419, 924, 968
CviQI GTAC 2 cut(s) 35, 998
DdeI CTNAG 4 cut(s) 58, 74, 327, 738
DpnI GATC 4 cut(s) 218, 294, 981, 1011
DpnII GATC 4 cut(s) 216, 292, 979, 1009
EaeI YGGCCR 2 cut(s) 915, 1043
Eco130I CCWWGG 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 1031
Eco47I GGWCC 2 cut(s) 841, 950
Eco47III AGCGCT 1 cut(s) 433
EcoO109I RGGNCCY 2 cut(s) 841, 950
EcoRI GAATTC 1 cut(s) 462
EcoRII CCWGG 3 cut(s) 457, 952, 1021
EcoT14I CCWWGG 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 6 cut(s) 98, 198, 400, 422, 927, 971
FaqI GGGAC 2 cut(s) 337, 689
FatI CATG 6 cut(s) 94, 194, 396, 418, 923, 967
FbaI TGATCA 1 cut(s) 216
FblI GTMKAC 1 cut(s) 340
Fnu4HI GCNGC 1 cut(s) 853
FokI GGATG 1 cut(s) 239
Fsp4HI GCNGC 1 cut(s) 853
GlaI GCGC 1 cut(s) 433
GluI GCNGC 1 cut(s) 853
GsaI CCCAGC 1 cut(s) 66
GsuI CTGGAG 1 cut(s) 983
HaeII RGCGCY 1 cut(s) 435
HaeIII GGCC 4 cut(s) 41, 917, 931, 1045
HapII CCGG 1 cut(s) 393
HhaI GCGC 1 cut(s) 434
Hin1II CATG 6 cut(s) 98, 198, 400, 422, 927, 971
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 4 cut(s) 78, 325, 404, 827
HpaII CCGG 1 cut(s) 393
HphI GGTGA 1 cut(s) 848
Hpy166II GTNNAC 4 cut(s) 250, 341, 415, 959
Hpy188I TCNGA 3 cut(s) 169, 741, 883
Hpy188III TCNNGA 4 cut(s) 329, 563, 983, 1005
Hpy8I GTNNAC 4 cut(s) 250, 341, 415, 959
HpyAV CCTTC 2 cut(s) 3, 980
HpyCH4III ACNGT 3 cut(s) 157, 338, 1002
HpyCH4IV ACGT 1 cut(s) 454
HpyCH4V TGCA 6 cut(s) 198, 415, 479, 635, 852, 936
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 641
HpyF3I CTNAG 4 cut(s) 58, 74, 327, 738
HpySE526I ACGT 1 cut(s) 454
Hsp92II CATG 6 cut(s) 98, 198, 400, 422, 927, 971
HspAI GCGC 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 4 cut(s) 216, 292, 979, 1009
LmnI GCTCC 1 cut(s) 964
Lsp1109I GCAGC 1 cut(s) 864
LweI GCATC 2 cut(s) 519, 1039
MaeII ACGT 1 cut(s) 454
MaeIII GTNAC 1 cut(s) 517
MalI GATC 4 cut(s) 218, 294, 981, 1011
MboI GATC 4 cut(s) 216, 292, 979, 1009
MboII GAAGA 3 cut(s) 368, 601, 754
MflI RGATCY 3 cut(s) 292, 979, 1009
MhlI GDGCHC 2 cut(s) 417, 562
MlsI TGGCCA 1 cut(s) 917
MluCI AATT 7 cut(s) 202, 309, 346, 462, 939, 991, 1031
MluNI TGGCCA 1 cut(s) 917
MlyI GAGTC 1 cut(s) 319
MmeI TCCRAC 1 cut(s) 259
Mox20I TGGCCA 1 cut(s) 917
MscI TGGCCA 1 cut(s) 917
MseI TTAA 2 cut(s) 569, 911
MslI CAYNNNNRTG 2 cut(s) 515, 788
Msp20I TGGCCA 1 cut(s) 917
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 1 cut(s) 393
MspR9I CCNGG 4 cut(s) 393, 459, 954, 1023
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 3 cut(s) 459, 954, 1023
MwoI GCNNNNNNNGC 2 cut(s) 38, 641
NciI CCSGG 1 cut(s) 393
NcoI CCATGG 1 cut(s) 418
NdeII GATC 4 cut(s) 216, 292, 979, 1009
NlaIII CATG 6 cut(s) 98, 198, 400, 422, 927, 971
NlaIV GGNNCC 2 cut(s) 843, 951
NmuCI GTSAC 1 cut(s) 517
NspI RCATGY 2 cut(s) 198, 400
PaeI GCATGC 1 cut(s) 400
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 3 cut(s) 78, 404, 827
PflMI CCANNNNNTGG 1 cut(s) 924
PfoI TCCNGGA 1 cut(s) 457
PkrI GCNGC 1 cut(s) 854
PleI GAGTC 1 cut(s) 319
PpsI GAGTC 1 cut(s) 319
PpuMI RGGWCCY 2 cut(s) 841, 950
PsiI TTATAA 3 cut(s) 308, 780, 807
Psp5II RGGWCCY 2 cut(s) 841, 950
Psp6I CCWGG 3 cut(s) 457, 952, 1021
PspFI CCCAGC 1 cut(s) 62
PspGI CCWGG 3 cut(s) 457, 952, 1021
PspN4I GGNNCC 2 cut(s) 843, 951
PspPI GGNCC 2 cut(s) 841, 950
PspPPI RGGWCCY 2 cut(s) 841, 950
PsrI GAACNNNNNNTAC 2 cut(s) 324, 356
PsuI RGATCY 3 cut(s) 292, 979, 1009
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 2 cut(s) 36, 999
RsaNI GTAC 2 cut(s) 35, 998
RseI CAYNNNNRTG 2 cut(s) 515, 788
SaqAI TTAA 2 cut(s) 569, 911
SatI GCNGC 1 cut(s) 853
Sau3AI GATC 4 cut(s) 216, 292, 979, 1009
Sau96I GGNCC 2 cut(s) 841, 950
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 1 cut(s) 319
ScrFI CCNGG 4 cut(s) 393, 459, 954, 1023
SduI GDGCHC 2 cut(s) 417, 562
SfaNI GCATC 2 cut(s) 519, 1039
SfcI CTRYAG 3 cut(s) 266, 652, 1067
SinI GGWCC 2 cut(s) 841, 950
SmiMI CAYNNNNRTG 2 cut(s) 515, 788
SphI GCATGC 1 cut(s) 400
Sse9I AATT 7 cut(s) 202, 309, 346, 462, 939, 991, 1031
SspI AATATT 1 cut(s) 135
StyD4I CCNGG 4 cut(s) 391, 457, 952, 1021
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 3 cut(s) 157, 338, 1002
TaiI ACGT 1 cut(s) 457
TasI AATT 7 cut(s) 202, 309, 346, 462, 939, 991, 1031
TatI WGTACW 2 cut(s) 34, 997
TfiI GAWTC 3 cut(s) 78, 404, 827
Tru1I TTAA 2 cut(s) 569, 911
Tru9I TTAA 2 cut(s) 569, 911
TscAI CASTG 1 cut(s) 867
TseFI GTSAC 1 cut(s) 517
TseI GCWGC 1 cut(s) 852
Tsp45I GTSAC 1 cut(s) 517
TspDTI ATGAA 3 cut(s) 83, 540, 819
TspGWI ACGGA 1 cut(s) 694
TspRI CASTG 1 cut(s) 867
Van91I CCANNNNNTGG 1 cut(s) 924
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 2 cut(s) 841, 950
XapI RAATTY 2 cut(s) 462, 991
XceI RCATGY 2 cut(s) 198, 400
XcmI CCANNNNNNNNNTGG 1 cut(s) 768
XmiI GTMKAC 1 cut(s) 340
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.