FvH4_1g25590

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002948
Physical Location & Seq
Forward (+)
17359291 .. 17367509
8219 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25590.t2

Sequence Viewer

Length: 1074 bp
ATGATGGAGGGAAGGAGATGGTTGCTGTTACTGGCACTAGTGGCTTGCCTGTTTGCTTCTTCTTCTGGGCGAGAATGGAAGTACAAGCACAAGGTTCATAGCCAGAGCCCTGTCTACAATCATACTCTTGCCACAATATTGGTTCGCTATGCCTCCACGGTGTATTTGTCAGATTTGACAGAGCTGTTTTCTTGGACATGTGACAGATGTAATGGCTTAATTAAGGACTTTCAAATGATGGAGCTAATTGTGGACGTCCAGCATTGCTTACAGGCATTTGTTGGAGTGGCAACAGATCCAAATGCTATTATAATTGCATTTAAAGGGACTCAGGGAAACAGTATACAGAATTGGATTGAAGACTTATTTTGGAAACAACTTGATATAGATTACCCGGGCATGCCTGATGCAATGGTGCACCATGGGTTTTATAATGCATACCACAACACAACCATACGACCTGGAATTCTGAATGCTGTTGCAAGAGCCAAAGAGTTCTACGGAGATCTTGGCATCACAGTGACAGGGCACTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATTTAAGGGTTAATCAAAAAGAGGAAAATGTTCAAGTGATGACATTTGGACAACCTCGAATAGGTAATGCGATATTTGCTACTTACTATAGCGAAATTGTGCCGAACACAATCCGAGTTACAAATGGAAATGATGTTGTGCCTCATTTGCCTCCATACTACACTTATTTTCCTCAGAAGACATACCACCACTTCCCAAGAGAGGTGTGGCTTTATGACATTGGATTGCGAAGTCTTGTTTATCAGGTTGAGAAAATCTGTGATGATTCGGGTGAGGACCCAACCTGCAGCAGGTCAGTGATGGGGAACAGCATTTCAGACCATTTAGTTTATTATGGTGTCGAACTAAAGGACGAGACATGGAGACCATGCAAAATTGTGATGCTTCCTGGTTTACTGGATTATGGAAGTTCAGATCTGGAAGGAAATTTTGTTCTGTCCAGAGACCATGCAACTCCTGTTTTCAAATTGAAAATACAGTCAGATGCTGGGGGAAGACCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

358

Amino Acids

40.38

Weight (kDa)

5.99

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 104 - 242 1.1e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 311, 432
AatII GACGTC 1 cut(s) 258
Acc36I ACCTGC 2 cut(s) 849, 860
AccI GTMKAC 2 cut(s) 114, 343
AclWI GGATC 1 cut(s) 290
AcsI RAATTY 2 cut(s) 465, 994
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 3 cut(s) 629, 769, 858
AflIII ACRYGT 1 cut(s) 197
AgsI TTSAA 5 cut(s) 233, 359, 602, 1033, 1039
AhlI ACTAGT 1 cut(s) 37
AjnI CCWGG 2 cut(s) 460, 955
AloI GAACNNNNNNTCC 2 cut(s) 984, 1016
AluBI AGCT 2 cut(s) 184, 244
AluI AGCT 2 cut(s) 184, 244
Alw21I GWGCWC 2 cut(s) 420, 565
Alw26I GTCTC 3 cut(s) 917, 925, 1005
Alw44I GTGCAC 1 cut(s) 416
AlwI GGATC 1 cut(s) 290
AlwNI CAGNNNCTG 1 cut(s) 1055
Ama87I CYCGRG 1 cut(s) 394
ApaLI GTGCAC 1 cut(s) 416
ApeKI GCWGC 1 cut(s) 855
ApoI RAATTY 2 cut(s) 465, 994
Asp700I GAANNNNTTC 1 cut(s) 597
AspS9I GGNCC 1 cut(s) 844
AsuC2I CCSGG 2 cut(s) 395, 396
AsuHPI GGTGA 1 cut(s) 851
AvaI CYCGRG 1 cut(s) 394
AvaII GGWCC 1 cut(s) 844
BaeGI GKGCMC 2 cut(s) 420, 531
BanII GRGCYC 1 cut(s) 110
BbsI GAAGAC 3 cut(s) 366, 752, 1069
Bbv12I GWGCWC 2 cut(s) 420, 565
BbvI GCAGC 1 cut(s) 867
BccI CCATC 3 cut(s) 12, 232, 862
BciT130I CCWGG 2 cut(s) 462, 957
BcnI CCSGG 2 cut(s) 395, 396
BcoDI GTCTC 3 cut(s) 917, 925, 1005
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 3 cut(s) 655, 853, 1070
BfuAI ACCTGC 2 cut(s) 849, 860
BglII AGATCT 2 cut(s) 505, 982
BisI GCNGC 1 cut(s) 856
BlsI GCNGC 1 cut(s) 857
Bme1390I CCNGG 4 cut(s) 395, 396, 462, 957
Bme18I GGWCC 1 cut(s) 844
BmeT110I CYCGRG 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 844
BmiI GGNNCC 1 cut(s) 846
BmrFI CCNGG 4 cut(s) 395, 396, 462, 957
BmsI GCATC 4 cut(s) 397, 522, 939, 1042
BpiI GAAGAC 3 cut(s) 366, 752, 1069
BpuMI CCSGG 2 cut(s) 395, 396
BsaHI GRCGYC 1 cut(s) 255
BsaI GGTCTC 2 cut(s) 925, 1005
BsaJI CCNNGG 3 cut(s) 156, 394, 421
Bsc4I CCNNNNNNNGG 3 cut(s) 629, 769, 858
Bse1I ACTGG 2 cut(s) 36, 969
Bse3DI GCAATG 3 cut(s) 262, 417, 552
BseBI CCWGG 2 cut(s) 462, 957
BseDI CCNNGG 3 cut(s) 156, 394, 421
BseLI CCNNNNNNNGG 3 cut(s) 629, 769, 858
BseMI GCAATG 3 cut(s) 262, 417, 552
BseMII CTCAG 2 cut(s) 344, 755
BseNI ACTGG 2 cut(s) 36, 969
BseSI GKGCMC 2 cut(s) 420, 531
BseXI GCAGC 1 cut(s) 867
BseYI CCCAGC 1 cut(s) 1055
BsiHKAI GWGCWC 2 cut(s) 420, 565
BsiHKCI CYCGRG 1 cut(s) 394
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 3 cut(s) 629, 769, 858
BsmAI GTCTC 3 cut(s) 917, 925, 1005
BsmFI GGGAC 1 cut(s) 340
BsmI GAATGC 1 cut(s) 478
Bso31I GGTCTC 2 cut(s) 925, 1005
BsoBI CYCGRG 1 cut(s) 394
Bsp1286I GDGCHC 4 cut(s) 110, 420, 531, 565
Bsp143I GATC 3 cut(s) 295, 505, 982
Bsp19I CCATGG 1 cut(s) 421
BspCNI CTCAG 2 cut(s) 343, 754
BspLI GGNNCC 1 cut(s) 846
BspMAI CTGCAG 1 cut(s) 857
BspMI ACCTGC 2 cut(s) 849, 860
BspPI GGATC 1 cut(s) 290
BspTNI GGTCTC 2 cut(s) 925, 1005
BsrDI GCAATG 3 cut(s) 262, 417, 552
BsrI ACTGG 2 cut(s) 36, 969
BssECI CCNNGG 3 cut(s) 156, 394, 421
BssMI GATC 3 cut(s) 295, 505, 982
BssNAI GTATAC 1 cut(s) 344
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 1 cut(s) 421
Bst1107I GTATAC 1 cut(s) 344
Bst2UI CCWGG 2 cut(s) 462, 957
Bst4CI ACNGT 4 cut(s) 160, 341, 520, 1047
BstACI GRCGYC 1 cut(s) 255
BstC8I GCNNGC 2 cut(s) 46, 401
BstDEI CTNAG 2 cut(s) 330, 741
BstDSI CCRYGG 2 cut(s) 156, 421
BstENI CCTNNNNNAGG 2 cut(s) 627, 856
BstKTI GATC 3 cut(s) 298, 508, 985
BstMAI GTCTC 3 cut(s) 917, 925, 1005
BstMBI GATC 3 cut(s) 295, 505, 982
BstMWI GCNNNNNNNGC 3 cut(s) 41, 644, 715
BstNI CCWGG 2 cut(s) 462, 957
BstNSI RCATGY 2 cut(s) 201, 403
BstSCI CCNGG 4 cut(s) 393, 394, 460, 955
BstSFI CTRYAG 3 cut(s) 655, 853, 1070
BstSLI GKGCMC 2 cut(s) 420, 531
BstV1I GCAGC 1 cut(s) 867
BstV2I GAAGAC 3 cut(s) 366, 752, 1069
BstX2I RGATCY 3 cut(s) 295, 505, 982
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 3 cut(s) 295, 505, 982
BstZ17I GTATAC 1 cut(s) 344
BtgI CCRYGG 2 cut(s) 156, 421
BtsIMutI CAGTG 2 cut(s) 525, 870
BveI ACCTGC 2 cut(s) 849, 860
Cac8I GCNNGC 2 cut(s) 46, 401
CaiI CAGNNNCTG 1 cut(s) 1055
Cfr13I GGNCC 1 cut(s) 844
Cfr9I CCCGGG 1 cut(s) 394
Csp6I GTAC 1 cut(s) 82
CviAII CATG 6 cut(s) 198, 400, 422, 927, 936, 1016
CviJI RGCY 9 cut(s) 44, 102, 108, 184, 216, 244, 488, 551, 778
CviKI_1 RGCY 9 cut(s) 44, 102, 108, 184, 216, 244, 488, 551, 778
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 2 cut(s) 330, 741
DpnI GATC 3 cut(s) 297, 507, 984
DpnII GATC 3 cut(s) 295, 505, 982
DraI TTTAAA 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 421
Eco24I GRGCYC 1 cut(s) 110
Eco31I GGTCTC 2 cut(s) 925, 1005
Eco47I GGWCC 1 cut(s) 844
Eco88I CYCGRG 1 cut(s) 394
EcoNI CCTNNNNNAGG 2 cut(s) 627, 856
EcoO109I RGGNCCY 1 cut(s) 844
EcoRI GAATTC 1 cut(s) 465
EcoRII CCWGG 2 cut(s) 460, 955
EcoT14I CCWWGG 1 cut(s) 421
EcoT22I ATGCAT 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 110
ErhI CCWWGG 1 cut(s) 421
FaeI CATG 6 cut(s) 201, 403, 425, 930, 939, 1019
FaqI GGGAC 1 cut(s) 340
FatI CATG 6 cut(s) 197, 399, 421, 926, 935, 1015
FblI GTMKAC 2 cut(s) 114, 343
Fnu4HI GCNGC 1 cut(s) 856
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 1 cut(s) 856
FspBI CTAG 1 cut(s) 38
GluI GCNGC 1 cut(s) 856
GsaI CCCAGC 1 cut(s) 1059
HapII CCGG 1 cut(s) 395
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 6 cut(s) 201, 403, 425, 930, 939, 1019
HinfI GANTC 2 cut(s) 328, 833
HpaII CCGG 1 cut(s) 395
HphI GGTGA 1 cut(s) 851
Hpy166II GTNNAC 5 cut(s) 115, 253, 344, 418, 962
Hpy188I TCNGA 7 cut(s) 172, 471, 683, 744, 886, 982, 1051
Hpy188III TCNNGA 3 cut(s) 566, 986, 1008
Hpy8I GTNNAC 5 cut(s) 115, 253, 344, 418, 962
HpyAV CCTTC 2 cut(s) 6, 983
HpyCH4III ACNGT 4 cut(s) 160, 341, 520, 1047
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 8 cut(s) 317, 410, 418, 437, 482, 855, 939, 1019
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 644, 715
HpyF3I CTNAG 2 cut(s) 330, 741
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 6 cut(s) 201, 403, 425, 930, 939, 1019
Kzo9I GATC 3 cut(s) 295, 505, 982
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 1 cut(s) 867
LweI GCATC 4 cut(s) 397, 522, 939, 1042
MaeI CTAG 1 cut(s) 38
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 4 cut(s) 27, 200, 520, 685
MalI GATC 3 cut(s) 297, 507, 984
MboI GATC 3 cut(s) 295, 505, 982
MboII GAAGA 5 cut(s) 51, 54, 371, 757, 1074
MflI RGATCY 3 cut(s) 295, 505, 982
MhlI GDGCHC 4 cut(s) 110, 420, 531, 565
MluCI AATT 9 cut(s) 219, 246, 312, 349, 465, 663, 942, 994, 1034
MlyI GAGTC 1 cut(s) 322
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 9 cut(s) 163, 533, 583, 633, 720, 729, 750, 763, 835
Mph1103I ATGCAT 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 597
MseI TTAA 5 cut(s) 218, 222, 321, 572, 579
MslI CAYNNNNRTG 1 cut(s) 518
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 4 cut(s) 395, 396, 462, 957
Mva1269I GAATGC 1 cut(s) 478
MvaI CCWGG 2 cut(s) 462, 957
MwoI GCNNNNNNNGC 3 cut(s) 41, 644, 715
NciI CCSGG 2 cut(s) 395, 396
NcoI CCATGG 1 cut(s) 421
NdeII GATC 3 cut(s) 295, 505, 982
NlaIII CATG 6 cut(s) 201, 403, 425, 930, 939, 1019
NlaIV GGNNCC 1 cut(s) 846
NmuCI GTSAC 2 cut(s) 200, 520
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 2 cut(s) 201, 403
PacI TTAATTAA 1 cut(s) 222
PaeI GCATGC 1 cut(s) 403
PciI ACATGT 1 cut(s) 197
PctI GAATGC 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 597
PfeI GAWTC 1 cut(s) 833
PkrI GCNGC 1 cut(s) 857
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 844
PscI ACATGT 1 cut(s) 197
PsiI TTATAA 2 cut(s) 311, 432
Psp5II RGGWCCY 1 cut(s) 844
Psp6I CCWGG 2 cut(s) 460, 955
PspFI CCCAGC 1 cut(s) 1055
PspGI CCWGG 2 cut(s) 460, 955
PspN4I GGNNCC 1 cut(s) 846
PspPI GGNCC 1 cut(s) 844
PspPPI RGGWCCY 1 cut(s) 844
PstI CTGCAG 1 cut(s) 857
PstNI CAGNNNCTG 1 cut(s) 1055
PsuI RGATCY 3 cut(s) 295, 505, 982
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
RseI CAYNNNNRTG 1 cut(s) 518
SaqAI TTAA 5 cut(s) 218, 222, 321, 572, 579
SatI GCNGC 1 cut(s) 856
Sau3AI GATC 3 cut(s) 295, 505, 982
Sau96I GGNCC 1 cut(s) 844
SchI GAGTC 1 cut(s) 322
ScrFI CCNGG 4 cut(s) 395, 396, 462, 957
SduI GDGCHC 4 cut(s) 110, 420, 531, 565
SfaNI GCATC 4 cut(s) 397, 522, 939, 1042
SfcI CTRYAG 3 cut(s) 655, 853, 1070
SinI GGWCC 1 cut(s) 844
SmaI CCCGGG 1 cut(s) 396
SmiMI CAYNNNNRTG 1 cut(s) 518
SpeI ACTAGT 1 cut(s) 37
SphI GCATGC 1 cut(s) 403
Sse9I AATT 9 cut(s) 219, 246, 312, 349, 465, 663, 942, 994, 1034
SspI AATATT 1 cut(s) 138
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 4 cut(s) 393, 394, 460, 955
StyI CCWWGG 1 cut(s) 421
TaaI ACNGT 4 cut(s) 160, 341, 520, 1047
TaiI ACGT 1 cut(s) 258
TaqI TCGA 2 cut(s) 625, 909
TasI AATT 9 cut(s) 219, 246, 312, 349, 465, 663, 942, 994, 1034
TatI WGTACW 1 cut(s) 81
TfiI GAWTC 1 cut(s) 833
Tru1I TTAA 5 cut(s) 218, 222, 321, 572, 579
Tru9I TTAA 5 cut(s) 218, 222, 321, 572, 579
TscAI CASTG 2 cut(s) 525, 870
TseFI GTSAC 2 cut(s) 200, 520
TseI GCWGC 1 cut(s) 855
Tsp45I GTSAC 2 cut(s) 200, 520
TspDTI ATGAA 2 cut(s) 86, 543
TspGWI ACGGA 1 cut(s) 516
TspMI CCCGGG 1 cut(s) 394
TspRI CASTG 2 cut(s) 525, 870
VneI GTGCAC 1 cut(s) 416
VpaK11BI GGWCC 1 cut(s) 844
XagI CCTNNNNNAGG 2 cut(s) 627, 856
XapI RAATTY 2 cut(s) 465, 994
XceI RCATGY 2 cut(s) 201, 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 771
XmaI CCCGGG 1 cut(s) 394
XmiI GTMKAC 2 cut(s) 114, 343
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 1 cut(s) 38
ZraI GACGTC 1 cut(s) 256
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.